(K128780) Támogató: Nemzeti Kutatás, Fejlesztés és Innovációs Iroda
Szakterületek:
Klinikai orvostan
Genetic alterations are well known to be related to the pathogenesis and prognosis
of papillary thyroid carcinoma (PTC). Some miRNA expression dysregulations have previously
been described in the context of cancer development including thyroid carcinoma. In
our study, we performed original molecular diagnostics on tissue samples related to
our own patients. We aimed to identify all dysregulated miRNAs in potential association
with PTC development via sequencing much higher numbers of control-matched PTC tissue
samples and analyzing a wider variety of miRNA types than previous studies. We analyzed
the expression levels of 2656 different human miRNAs in the context of 236 thyroid
tissue samples (118 tumor and control pairs) related to anonymized PTC cases. Also,
KEGG pathway enrichment analysis and GO framework analysis were used to establish
the links between miRNA dysregulation and certain biological processes, pathways of
signaling, molecular functions, and cellular components. A total of 30 significant
differential miRNA expressions with at least ±1 log2 fold change were found related
to PTC including, e.g., miR-551b, miR-146b, miR-221, miR-222, and miR-375, among others,
being highly upregulated, as well as miR-873 and miR-204 being downregulated. In addition,
we identified miRNA patterns in vast databases (KEGG and GO) closely similar to that
of PTC including, e.g., miRNA patterns of prostate cancer, HTLV infection, HIF-1 signaling,
cellular responses to growth factor stimulus and organic substance, and negative regulation
of gene expression. We also found 352 potential associations between certain miRNA
expressions and states of clinicopathological variables. Our findings—supported by
the largest case number of original matched-control PTC–miRNA relation research—suggest
a distinct miRNA expression profile in PTC that could contribute to a deeper understanding
of the underlying molecular mechanisms promoting the pathogenesis of the disease.
Moreover, significant miRNA expression deviations and their signaling pathways in
PTC presented in our study may serve as potential biomarkers for PTC diagnosis and
prognosis or even therapeutic targets in the future.
