DisProt (URL: https://disprot.org) is the gold standard database for intrinsically
disordered proteins and regions, providing valuable information about their functions.
The latest version of DisProt brings significant advancements, including a broader
representation of functions and an enhanced curation process. These improvements aim
to increase both the quality of annotations and their coverage at the sequence level.
Higher coverage has been achieved by adopting additional evidence codes. Quality of
annotations has been improved by systematically applying Minimum Information About
Disorder Experiments (MIADE) principles and reporting all the details of the experimental
setup that could potentially influence the structural state of a protein. The DisProt
database now includes new thematic datasets and has expanded the adoption of Gene
Ontology terms, resulting in an extensive functional repertoire which is automatically
propagated to UniProtKB. Finally, we show that DisProt's curated annotations strongly
correlate with disorder predictions inferred from AlphaFold2 pLDDT (predicted Local
Distance Difference Test) confidence scores. This comparison highlights the utility
of DisProt in explaining apparent uncertainty of certain well-defined predicted structures,
which often correspond to folding-upon-binding fragments. Overall, DisProt serves
as a comprehensive resource, combining experimental evidence of disorder information
to enhance our understanding of intrinsically disordered proteins and their functional
implications.Graphical Abstract
