A comprehensive study of the Ca2+ ion binding of fluorescently labelled BAPTA analogues

Csomos, Attila [Csomos, Attila (Kémia), szerző]; Kontra, Bence; Jancsó, Attila [Jancsó, Attila (Bioszervetlen kémia), szerző]; Galbács, Gábor [Galbács, Gábor (analitikai kémia), szerző] Szervetlen és Analitikai Kémiai Tanszék (SZTE / TTIK / KI); Deme, Ruth [Deme, Ruth (Szerves-és gyógys...), szerző]; Kele, Zoltán [Kele, Zoltán (Kémiai analitika,...), szerző]; Rózsa, Balázs József [Rózsa J., Balázs (Idegtudomány, fizika), szerző] Információs Technológiai és Bionikai Kar (PPKE); Kovács, Ervin ✉ [Kovács, Ervin (Szerves kémia), szerző] Anyag- és Környezetkémiai Intézet (TTK); Polimer Kémiai Kutatócsoport (TTK / AKI); Mucsi, Zoltán ✉ [Mucsi, Zoltán (szerves kémia), szerző]

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: EUROPEAN JOURNAL OF ORGANIC CHEMISTRY 1434-193X 1099-0690 2021 (37) pp. 5248-5261 2021
  • SJR Scopus - Physical and Theoretical Chemistry: Q1
Azonosítók
Szakterületek:
    Since its development, the ionophore BAPTA (1,2?bis(2?aminophenoxy)-ethane?N,N,N?,N??tetraacetic acid) has been used unchanged in calcium sensing applications. In this work we present a comprehensive experimental and theoretical study of novel alterations in the structure of BAPTA, with a focus on the systematic modification of the chain connecting the two aromatic rings of the molecule (denoted as ?linker?). A bis-(diethylamino)xantene fluorophore was also attached to the structures in a fixed position and the structure-fluorescence response relationship of these molecules was investigated in addition. The effect of the length of the linker, the number of O atoms in this chain and even the removal of one of the rings was tested; these all proved to significantly alter the characteristics of the compounds. For example, it was found that the second aromatic ring of BAPTA is not essential for the turn-on of the fluorescence. We also demonstrated that successful sensing can be realized even by replacing the chain with a single oxygen atom, which suggests the availability of a new calcium binding mode of the chelator. The reliable turn-on characteristic, the steep Ca 2+ fluorescence titration curve and the intense fluorescence emission combine to make this compound a prospective candidate as a calcium sensing molecular probe in diagnostic neurobiological applications.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2021-10-17 15:11