(Higher Education Institutional Excellence Programme of the Ministry of Human Capacities
in Hungary)
Szakterületek:
Kémiai tudományok
Synthesis and multiple STED imaging applications of four, red-emitting (610–670 nm),
tetrazine-functionalized fluorescent probes (CBRD = Chemical
Biology Research group Dye 1–4) with large Stokes-shift is
presented. Present studies revealed the super-resolution microscopy applicability
of the probes as demonstrated through bioorthogonal labeling scheme of cytoskeletal
proteins actin and keratin-19, and mitochondrial protein TOMM20. Furthermore, super-resolved
images of insulin receptors in live-cell bioorthogonal labeling schemes through a
genetically encoded cyclooctynylated non-canonical amino acid are also presented.
The large Stokes-shifts and the wide spectral bands of the probes enabled the use
of two common depletion lasers (660 nm and 775 nm). The probes were also found suitable
for super-resolution microscopy in combination with two-photon excitation (2P-STED)
resulting in improved spatial resolution. One of the dyes was also used together with
two commercial dyes in the three-color STED imaging of intracellular structures.
