TY - JOUR AU - Alaoui-Sosse, Badr AU - Ozaki, Shinji AU - Barriquand, Lionel AU - De Luca, Daniele AU - Cennamo, Paola AU - Valot, Benoit AU - Alaoui-Sosse, Laurence AU - Bourgeade, Pascale AU - Bousta, Faisl AU - Aleya, Lotfi AU - Pfendler, Stéphane TI - Assessment of microbial communities colonizing the Azé prehistoric cave JF - JOURNAL OF CULTURAL HERITAGE J2 - J CULT HERIT VL - 59 PY - 2023 SP - 1 EP - 9 PG - 9 SN - 1296-2074 DO - 10.1016/j.culher.2022.10.014 UR - https://m2.mtmt.hu/api/publication/33256633 ID - 33256633 N1 - Funding Agency and Grant Number: Ministere de la Culture et de la Communication (France); Laboratoire de Recherche des Monuments Historiques (LRMH, Paris); l'Association Culturelle du Site d'Aze Funding text: First of all, we are grateful to the curators of the Aze Cave, who kindly gave us permission to access the cave and to carry out all our field experiments. We also thank the Ministere de la Culture et de la Communication (France), the Laboratoire de Recherche des Monuments Historiques (LRMH, Paris) and l'Association Culturelle du Site d'Aze for their financial contribution. LA - English DB - MTMT ER - TY - JOUR AU - Lange-Enyedi, Nóra AU - Kériné Borsodi, Andrea AU - Németh, Péter AU - Czuppon, György AU - Kovács, Ivett AU - Leél-Őssy, Szabolcs AU - Dobosy, Péter AU - Felföldi, Tamás AU - Demény, Attila AU - Makk, Judit TI - Habitat-related variability in the morphological and taxonomic diversity of microbial communities in two Hungarian epigenic karst caves JF - FEMS MICROBIOLOGY ECOLOGY J2 - FEMS MICROBIOL ECOL VL - 99 PY - 2023 IS - 12 PG - 17 SN - 0168-6496 DO - 10.1093/femsec/fiad161 UR - https://m2.mtmt.hu/api/publication/34431117 ID - 34431117 N1 - Institute for Geological and Geochemical Research, HUN-REN Research Centre for Astronomy and Earth Sciences, Budaörsi út 45, Budapest, H-1112, Hungary Department of Microbiology, Institute of Biology, Faculty of Science, Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, H-1117, Hungary Institute of Aquatic Ecology, HUN-REN Centre for Ecological Research, Karolina út 29, Budapest, H-1113, Hungary Research Institute of Biomolecular and Chemical Engineering, Nanolab, University of Pannonia, Egyetem út 10, Veszprém, H-8200, Hungary Department of Physical and Applied Geology, Faculty of Science, Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, H-1117, Hungary Export Date: 8 February 2024 CODEN: FMECE Correspondence Address: Makk, J.; Department of Microbiology, Pázmány P. sétány 1/C, Hungary; email: makk.judit@ttk.elte.hu AB - The physical and chemical characteristics of the bedrock, along with the geological and hydrological conditions of karst caves may influence the taxonomic and functional diversity of prokaryotes. Most studies so far have focused on microbial communities of caves including only a few samples and have ignored the chemical heterogeneity of different habitat types such as sampling sites, dripping water, carbonate precipitates, cave walls, cave sediment and surface soils connected to the caves. The aim of the present study was to compare the morphology, the composition and physiology of the microbiota in caves with similar environmental parameters (temperature, host rock, elemental and mineral composition of speleothems) but located in different epigenic karst systems. Csodabogyós Cave and Baradla Cave (Hungary) were selected for the analysis of bacterial and archaeal communities using electron microscopy, amplicon sequencing, X-ray diffraction and mass spectroscopic techniques. The microbial communities belonged to the phyla Pseudomonadota, Acidobacteriota, Nitrospirota and Nitrososphaerota, and they showed site-specific variation in composition and diversity. The results indicate that morphological and physiological adaptations provide survival for microorganisms according to the environment. In epigenic karst caves, prokaryotes are prone to increase their adsorption surface, cooperate in biofilms, and implement chemolithoautotrophic growth with different electron-donors and acceptors available in the microhabitats. LA - English DB - MTMT ER - TY - JOUR AU - Michaelis, Claudia AU - Grohmann, Elisabeth TI - Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 12 PY - 2023 IS - 2 SP - 328 SN - 2079-6382 DO - 10.3390/antibiotics12020328 UR - https://m2.mtmt.hu/api/publication/33628317 ID - 33628317 N1 - Cited By :1 Export Date: 2 May 2023 Correspondence Address: Grohmann, E.; Faculty of Life Sciences and Technology, Germany; email: elisabeth.grohmann@bht-berlin.de AB - Most bacteria attach to biotic or abiotic surfaces and are embedded in a complex matrix which is known as biofilm. Biofilm formation is especially worrisome in clinical settings as it hinders the treatment of infections with antibiotics due to the facilitated acquisition of antibiotic resistance genes (ARGs). Environmental settings are now considered as pivotal for driving biofilm formation, biofilm-mediated antibiotic resistance development and dissemination. Several studies have demonstrated that environmental biofilms can be hotspots for the dissemination of ARGs. These genes can be encoded on mobile genetic elements (MGEs) such as conjugative and mobilizable plasmids or integrative and conjugative elements (ICEs). ARGs can be rapidly transferred through horizontal gene transfer (HGT) which has been shown to occur more frequently in biofilms than in planktonic cultures. Biofilm models are promising tools to mimic natural biofilms to study the dissemination of ARGs via HGT. This review summarizes the state-of-the-art of biofilm studies and the techniques that visualize the three main HGT mechanisms in biofilms: transformation, transduction, and conjugation. LA - English DB - MTMT ER - TY - JOUR AU - Skoog, Emilie J. AU - Fournier, Gregory P. AU - Bosak, Tanja TI - Assessing the Influence of HGT on the Evolution of Stress Responses in Microbial Communities from Shark Bay, Western Australia JF - GENES J2 - GENES-BASEL VL - 14 PY - 2023 IS - 12 SP - 2168 SN - 2073-4425 DO - 10.3390/genes14122168 UR - https://m2.mtmt.hu/api/publication/34421558 ID - 34421558 AB - Pustular microbial mats in Shark Bay, Western Australia, are modern analogs of microbial systems that colonized peritidal environments before the evolution of complex life. To understand how these microbial communities evolved to grow and metabolize in the presence of various environmental stresses, the horizontal gene transfer (HGT) detection tool, MetaCHIP, was used to identify the horizontal transfer of genes related to stress response in 83 metagenome-assembled genomes from a Shark Bay pustular mat. Subsequently, maximum-likelihood phylogenies were constructed using these genes and their most closely related homologs from other environments in order to determine the likelihood of these HGT events occurring within the pustular mat. Phylogenies of several stress-related genes—including those involved in response to osmotic stress, oxidative stress and arsenic toxicity—indicate a potentially long history of HGT events and are consistent with these transfers occurring outside of modern pustular mats. The phylogeny of a particular osmoprotectant transport gene reveals relatively recent adaptations and suggests interactions between Planctomycetota and Myxococcota within these pustular mats. Overall, HGT phylogenies support a potentially broad distribution in the relative timing of the HGT events of stress-related genes and demonstrate ongoing microbial adaptations and evolution in these pustular mat communities. LA - English DB - MTMT ER - TY - JOUR AU - Kériné Borsodi, Andrea AU - Anda, Dóra AU - Szabó, Attila AU - Megyes, Melinda AU - Krett, Gergely TI - Impacts of Different Habitats on the Composition of Bacterial Communities at the Discharging Endpoints of a Hypogene Thermal Karst System JF - GEOMICROBIOLOGY JOURNAL J2 - GEOMICROBIOL J VL - 39 PY - 2022 IS - 2 SP - 155 EP - 165 PG - 11 SN - 0149-0451 DO - 10.1080/01490451.2021.2023709 UR - https://m2.mtmt.hu/api/publication/32606386 ID - 32606386 N1 - Department of Microbiology, ELTE Eötvös Loránd University, Budapest, Hungary Centre for Ecological Research, Institute of Aquatic Ecology, Budapest, Hungary Export Date: 25 January 2022 Correspondence Address: Borsodi, A.K.1/c Pázmány Péter sétány, Hungary; email: borsodi.andrea@ttk.elte.hu LA - English DB - MTMT ER - TY - CHAP AU - Leél-Őssy, Szabolcs AU - Virág, Magdolna ED - Veress, Márton ED - Leél-Őssy, Szabolcs TI - The Caves of the Northeastern Part of the Transdanubian Mountains T2 - Cave and karst systems of Hungary PB - Springer Netherlands CY - Cham SN - 9783030929602 PY - 2022 SP - 361 EP - 413 PG - 53 DO - 10.1007/978-3-030-92960-2_12 UR - https://m2.mtmt.hu/api/publication/33041060 ID - 33041060 LA - English DB - MTMT ER - TY - JOUR AU - Sadiq, Faizan Ahmed AU - Hansen, Mads Frederik AU - Burmolle, Mette AU - Heyndrickx, Marc AU - Flint, Steve AU - Lu, Wenwei AU - Chen, Wei AU - Zhang, Hao TI - Trans-kingdom interactions in mixed biofilm communities JF - FEMS MICROBIOLOGY REVIEWS J2 - FEMS MICROBIOL REV VL - 46 PY - 2022 IS - 5 PG - 20 SN - 0168-6445 DO - 10.1093/femsre/fuac024 UR - https://m2.mtmt.hu/api/publication/33255813 ID - 33255813 N1 - Funding Agency and Grant Number: European Commission; National Natural Science Foundation of China Funding text: We acknowledge the European Commission and the National Natural Science Foundation of China for their financial support. AB - The microbial world represents a phenomenal diversity of microorganisms from different kingdoms of life, which occupy an impressive set of ecological niches. Most, if not all, microorganisms once colonize a surface develop architecturally complex surface-adhered communities, which we refer to as biofilms. They are embedded in polymeric structural scaffolds and serve as a dynamic milieu for intercellular communication through physical and chemical signalling. Deciphering microbial ecology of biofilms in various natural or engineered settings has revealed coexistence of microorganisms from all domains of life, including Bacteria, Archaea, and Eukarya. The coexistence of these dynamic microbes is not arbitrary, as a highly coordinated architectural setup and physiological complexity show ecological interdependence and myriads of underlying interactions. In this review, we describe how species from different kingdoms interact in biofilms and discuss the functional consequences of such interactions. We highlight metabolic advances of collaboration among species from different kingdoms, and advocate that these interactions are of great importance and need to be addressed in future research. Since trans-kingdom biofilms impact diverse contexts, ranging from complicated infections to efficient growth of plants, future knowledge within this field will be beneficial for medical microbiology, biotechnology, and our general understanding of microbial life in nature. LA - English DB - MTMT ER - TY - JOUR AU - Chen, Ying AU - Qiu, Kairui AU - Zhong, Ziyuan AU - Zhou, Tao TI - Influence of Environmental Factors on the Variability of Archaeal Communities in a Karst Wetland JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 12 PY - 2021 PG - 16 SN - 1664-302X DO - 10.3389/fmicb.2021.675665 UR - https://m2.mtmt.hu/api/publication/32350158 ID - 32350158 N1 - Funding Agency and Grant Number: National Natural Science Foundation of China [41662019]; Natural Science Foundation of Guangxi Zhuang Autonomous Region of China [2016GXNSFBA380197] Funding text: Funding. The financial support was received from the National Natural Science Foundation of China (grant no. 41662019) and the Natural Science Foundation of Guangxi Zhuang Autonomous Region of China (grant no. 2016GXNSFBA380197). AB - Archaea are ubiquitous and play an important role in elemental cycles in Earth's biosphere; but little is known about their diversity, distribution, abundance, and impact in karst environments. The present study investigated the effect of environmental factors on the variability of archaeal communities in the sediment of the Huixian karst wetland, the largest karst wetland in South China. Sediment cores were obtained from four sampling sites with different water depths and macrophyte inhabitants in both the winter of 2016 and the summer of 2018. The community analysis was based on PacBio sequencing and quantitative PCR of the archaeal 16S rRNA gene. The results showed that Euryarchaeota (57.4%) and Bathyarchaeota (38.7%) were dominant in all the samples. Methanogenic Methanosarcinales (25.1%) and Methanomicrobiales (13.7%), and methanotrophic archaea ANME-2d (9.0%) were the dominant Euryarchaeota; MCG-11 (16.5%), MCG-6 (9.1%), and MCG-5b (5.5%) were the dominant Bathyarchaeota. The community composition remained stable between summer and winter, and the vertical distributions of the archaeal phyla conformed to two patterns among the four sampling sites. In the winter samples, the archaeal 16S rRNA gene abundance was approximately 1.0E+10 copies/g of wet sediment and the Shannon index was 7.3 +/- 5, which were significantly higher than in the summer samples and in other karst environments. A correlation analysis showed that the moisture content and pH were the factors that mostly affected the archaeal communities. The prevalence of nitrate in the summer may be a key factor causing a significant decrease in archaeal abundance and diversity. Two features specific to karst environments, calcium-richness and weak alkalescence of the water supplies, may benefit the prevalence of bathyarchaeotal subgroups MCG-11, MCG-5b, and MCG-6. These results suggest that in karst wetlands, most of the archaea belong to clades that have significant roles in carbon turnover; their composition remains stable, but their abundance and diversity vary significantly from season to season. LA - English DB - MTMT ER - TY - JOUR AU - Lv, Jiali AU - Niu, Yangdan AU - Yuan, Ruiqiang AU - Wang, Shiqin TI - Different Responses of Bacterial and Archaeal Communities in River Sediments to Water Diversion and Seasonal Changes JF - MICROORGANISMS J2 - MICROORGANISMS VL - 9 PY - 2021 IS - 4 PG - 23 SN - 2076-2607 DO - 10.3390/microorganisms9040782 UR - https://m2.mtmt.hu/api/publication/32113773 ID - 32113773 N1 - Funding Agency and Grant Number: National Natural Science Foundation of China [42071037] Funding text: This research was funded by The National Natural Science Foundation of China, grant number No.42071037. AB - In recent years, different responses of archaea and bacteria to environmental changes have attracted increasing scientific interest. In the mid-latitude region, Fen River receives water transferred from the Yellow River, electrical conductivity (EC), concentrations of Cl- and Na+ in water, total phosphorus (TP), and Olsen phosphorus (OP) in sediments were significantly affected by water transfer. Meanwhile, temperature and oxidation-reduction potential (ORP) of water showed significant seasonal variations. Based on 16S rRNA high-throughput sequencing technology, the composition of bacteria and archaea in sediments was determined in winter and summer, respectively. Results showed that the dominance of bacterial core flora decreased and that of archaeal core flora increased after water diversion. The abundance and diversity of bacterial communities in river sediments were more sensitive to anthropogenic and naturally induced environmental changes than that of archaeal communities. Bacterial communities showed greater resistance than archaeal communities under long-term external disturbances, such as seasonal changes, because of rich species composition and complex community structure. Archaea were more stable than bacteria, especially under short-term drastic environmental disturbances, such as water transfer, due to their insensitivity to environmental changes. These results have important implications for understanding the responses of bacterial and archaeal communities to environmental changes in river ecosystems affected by water diversion. LA - English DB - MTMT ER - TY - JOUR AU - Quemeneur, Marianne AU - Mei, Nan AU - Monnin, Christophe AU - Postec, Anne AU - Wils, Laura AU - Bartoli, Manon AU - Guasco, Sophie AU - Pelletier, Bernard AU - Erauso, Gael TI - Procaryotic Diversity and Hydrogenotrophic Methanogenesis in an Alkaline Spring (La Crouen, New Caledonia) JF - MICROORGANISMS J2 - MICROORGANISMS VL - 9 PY - 2021 IS - 7 PG - 19 SN - 2076-2607 DO - 10.3390/microorganisms9071360 UR - https://m2.mtmt.hu/api/publication/32193288 ID - 32193288 N1 - Funding Agency and Grant Number: French Institute of Research for Development (IRD); INTERVIE CNRS INSU program; H2NAT project - Midi-Pyrenees Observatory (OMP, Toulouse); ANR MICROPRONY [19-CE02-0020-02] Funding text: This research was funded by the French Institute of Research for Development (IRD), the INTERVIE CNRS INSU program, the H2NAT project supported by the Midi-Pyrenees Observatory (OMP, Toulouse) and ANR MICROPRONY, grant number 19-CE02-0020-02. AB - (1) Background: The geothermal spring of La Crouen (New Caledonia) discharges warm (42 degrees C) alkaline water (pH similar to 9) enriched in dissolved nitrogen with traces of methane, but its microbial diversity has not yet been studied. (2) Methods: Cultivation-dependent and -independent methods (e.g., Illumina sequencing and quantitative PCR based on 16S rRNA gene) were used to describe the prokaryotic diversity of this spring. (3) Results: Prokaryotes were mainly represented by Proteobacteria (57% on average), followed by Cyanobacteria, Chlorofexi, and Candidatus Gracilibacteria (GN02/BD1-5) (each > 5%). Both potential aerobes and anaerobes, as well as mesophilic and thermophilic microorganisms, were identified. Some of them had previously been detected in continental hyperalkaline springs found in serpentinizing environments (The Cedars, Samail, Voltri, and Zambales ophiolites). Gammaproteobacteria, Ca. Gracilibacteria and Thermotogae were significantly more abundant in spring water than in sediments. Potential chemolithotrophs mainly included beta- and gammaproteobacterial genera of sulfate-reducers (Ca. Desulfobacillus), methylotrophs (Methyloversatilis), sulfur-oxidizers (Thiofaba, Thiovirga), or hydrogen-oxidizers (Hydrogenophaga). Methanogens (Methanobacteriales and Methanosarcinales) were the dominant Archaea, as found in serpentinization-driven and deep subsurface ecosystems. A novel alkaliphilic hydrogenotrophic methanogen (strain CAN) belonging to the genus Methanobacterium was isolated, suggesting that hydrogenotrophic methanogenesis occurs at La Crouen. LA - English DB - MTMT ER - TY - JOUR AU - Anda, Dóra AU - Szabó, Attila AU - Kovácsné Bodor, Petra AU - Makk, Judit AU - Felföldi, Tamás AU - Ács, Éva AU - Mádlné Szőnyi, Judit AU - Kériné Borsodi, Andrea TI - In situ modelling of biofilm formation in a hydrothermal spring cave JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 10 PY - 2020 IS - 1 PG - 9 SN - 2045-2322 DO - 10.1038/s41598-020-78759-4 UR - https://m2.mtmt.hu/api/publication/31746781 ID - 31746781 N1 - Department of Microbiology, ELTE Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, 1117, Hungary Danube Research Institute, Centre for Ecological Research, Karolina út 29, Budapest, 1113, Hungary Department of Geology, ELTE Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, 1117, Hungary Faculty of Water Sciences, National University of Public Service, Bajcsy-Zsilinszky utca, 12-14, Baja, 6500, Hungary Cited By :3 Export Date: 19 May 2022 Correspondence Address: Anda, D.; Department of Microbiology, Pázmány P. sétány 1/C, Hungary; email: andadora@caesar.elte.hu Correspondence Address: Borsodi, A.K.; Department of Microbiology, Pázmány P. sétány 1/C, Hungary; email: borsodi.andrea@ttk.elte.hu AB - Attachment of microorganisms to natural or artificial surfaces and the development of biofilms are complex processes which can be influenced by several factors. Nevertheless, our knowledge on biofilm formation in karstic environment is quite incomplete. The present study aimed to examine biofilm development for a year under controlled conditions in quasi-stagnant water of a hydrothermal spring cave located in the Buda Thermal Karst System (Hungary). Using a model system, we investigated how the structure of the biofilm is formed from the water and also how the growth rate of biofilm development takes place in this environment. Besides scanning electron microscopy, next-generation DNA sequencing was used to reveal the characteristic taxa and major shifts in the composition of the bacterial communities. Dynamic temporal changes were observed in the structure of bacterial communities. Bacterial richness and diversity increased during the biofilm formation, and 9-12 weeks were needed for the maturation. Increasing EPS production was also observed from the 9-12 weeks. The biofilm was different from the water that filled the cave pool, in terms of the taxonomic composition and metabolic potential of microorganisms. In these karstic environments, the formation of mature biofilm appears to take place relatively quickly, in a few months. LA - English DB - MTMT ER - TY - JOUR AU - Das, Sayak AU - Sherpa, Mingma Thundu AU - Najar, Ishfaq Nabi AU - Thakur, Nagendra TI - Prevalence of methanogens in the uncultured Sikkim hot spring solfataric mud archaeal microbiome JF - ENVIRONMENTAL SUSTAINABILITY J2 - ENVIRON SUSTAIN VL - . PY - 2020 SP - . SN - 2523-8922 DO - 10.1007/s42398-020-00133-x UR - https://m2.mtmt.hu/api/publication/31649323 ID - 31649323 LA - English DB - MTMT ER - TY - JOUR AU - Putri, Ade Lia AU - Sumerta, I Nyoman TI - SELECTIVE ISOLATION OF Dactylosporangium AND Micromonospora FROM THE SOIL OF KARST CAVE OF SIMEULUE ISLAND AND THEIR ANTIBACTERIAL POTENCY JF - BERITA BIOLOGI : JURNAL ILMU-ILMU HAYATI J2 - BERITA BIOLOGI VL - 19 PY - 2020 IS - 3A SP - 257 EP - 268 PG - 12 SN - 0126-1754 DO - 10.14203/beritabiologi.v19i3A.3933 UR - https://m2.mtmt.hu/api/publication/31797381 ID - 31797381 LA - English DB - MTMT ER - TY - JOUR AU - Virág, Magdolna AU - Molnár, Mihály AU - Braun, Mihály AU - Mindszenty, Andrea TI - Investigation of a flowstone-like historical indoor-travertine (Rudas Spa, Budapest, Hungary) using the 14C "bomb-peak" JF - RADIOCARBON J2 - RADIOCARBON VL - 62 PY - 2020 IS - 5 SP - 1419 EP - 1435 PG - 17 SN - 0033-8222 DO - 10.1017/RDC.2020.24 UR - https://m2.mtmt.hu/api/publication/31306981 ID - 31306981 N1 - Institute for Nuclear Research of the Hungarian Academy of Sciences (ATOMKI), Isotope Climatology and Environmental Research Centre, Debrecen, Hungary Department of Physical and Applied Geology, Eötvös Loránd University, Budapest, Hungary Export Date: 20 November 2020 CODEN: RACAA Correspondence Address: Virág, M.; Institute for Nuclear Research of the Hungarian Academy of Sciences (ATOMKI), Isotope Climatology and Environmental Research CentreHungary; email: virag.magdi@gmail.com Funding Agency and Grant Number: European UnionEuropean Union (EU); State of Hungary; European Regional Development FoundEuropean Union (EU) [GINOP-2.3.2-15-2016-00009]; New National Excellence Program of the Ministry of Human Capacities [UNKP-17-3]; OTKAOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [72590K] Funding text: The research was supported by the European Union and the State of Hungary, co-financed by the European Regional Development Found in the project of GINOP-2.3.2-15-2016-00009 `ICER'. This study also supported by the UNKP-17-3 New National Excellence Program of the Ministry of Human Capacities and the OTKA 72590K to A. Mindszenty. Thanks are due to Assoc. Prof. Sz. Leel-Ossy and G. Suranyi for the core-sampling, to the Budapest Thermal Waters and Spas Ltd. particularly to J. Janoshazi and A. Varallyai for the permission of sampling, and to G. Szalontai for discussions on the history of the technical management of the Spa. The authors are grateful to Assoc. Prof. J. Madl-Szonyi for thought-provoking discussions on the hydrogeology of the Buda Thermal Karst. Reviewers Dr. A.J. Timothy Jull's and Irka Hajdas's efforts to improve the manuscript are also greatly appreciated. Institute for Nuclear Research of the Hungarian Academy of Sciences (ATOMKI), Isotope Climatology and Environmental Research Centre, Debrecen, Hungary Department of Physical and Applied Geology, Eötvös Loránd University, Budapest, Hungary Export Date: 3 May 2021 CODEN: RACAA Correspondence Address: Virág, M.; Institute for Nuclear Research of the Hungarian Academy of Sciences (ATOMKI), Hungary; email: virag.magdi@gmail.com Institute for Nuclear Research of the Hungarian Academy of Sciences (ATOMKI), Isotope Climatology and Environmental Research Centre, Debrecen, Hungary Department of Physical and Applied Geology, Eötvös Loránd University, Budapest, Hungary Export Date: 28 May 2021 CODEN: RACAA Correspondence Address: Virág, M.; Institute for Nuclear Research of the Hungarian Academy of Sciences (ATOMKI), Hungary; email: virag.magdi@gmail.com AB - Travertine precipitated during the past ca. 120 years, from thermal waters in one of the historical Spas of the Buda Thermal Karst (Hungary) was investigated using radiocarbon (14C). The age of the deposit is based on the historic date of the structure on which the travertine was deposited. A textural study of the travertine buildup using a ~22-cm-long diamond-core crosscutting was undertaken. The original aim of the study was to improve our understanding of the controls and possibly also the rate of travertine-precipitation. In addition to characteristic, mm-scale, regular laminations, 0.5–1.0 cm dark-colored intervals were also observed in the core. Correlation of these latter textural changes with well-known changes in the water management of the Spa was greatly hindered by the lack of age data from the interior of the core. Therefore, in addition to the two known points (beginning in 1883 AD and ending 2004 AD) at least one age-datum point, somewhere inbetween, was necessary. Since the timespan of the core obviously included the 1960s of the last century, we expected that the 14C anomaly related to the atmospheric nuclear tests of those years could be detected by isotope-geochemistry. This paper gives a brief overview of the textural features of the investigated travertine and presents the dataset proving the incorporation of considerable amounts of atmospheric carbon in the carbonate precipitate, which, indeed, facilitated the indirect dating of the part of the core containing “bomb” 14C, and this helped us to unfold the factors controlling the observed textural changes of the travertine. LA - English DB - MTMT ER - TY - JOUR AU - Lange-Enyedi, Nóra AU - Anda, Dóra AU - Kériné Borsodi, Andrea AU - Szabó, Attila AU - Pál, Sára Eszter AU - Óvári, Mihály AU - Márialigeti, Károly AU - Kovácsné Bodor, Petra AU - Mádlné Szőnyi, Judit AU - Makk, Judit TI - Radioactive environment adapted bacterial communities constituting the biofilms of hydrothermal spring caves (Budapest, Hungary) JF - JOURNAL OF ENVIRONMENTAL RADIOACTIVITY J2 - J ENVIRON RADIOACTIV VL - 203 PY - 2019 SP - 8 EP - 17 PG - 10 SN - 0265-931X DO - 10.1016/j.jenvrad.2019.02.010 UR - https://m2.mtmt.hu/api/publication/30539729 ID - 30539729 N1 - Cited By :3 Export Date: 29 October 2019 AB - The thermal waters of Gellért Hill discharge area of the Buda Thermal Karst System (Hungary) are characterized by high (up to 1000 Bq/L) 222Rn-activity due to the radium-accumulating biogeochemical layers. Samples were taken from these ferruginous and calcareous layers developed on spring cave walls and water surface. Accumulation of potentially toxic metals (e.g. As, Hg, Pb, Sn, Sr, Zn) in the dense extracellular polymeric substance containing bacterial cells and remains was detected by inductively coupled plasma mass spectrometry. The comparison of bacterial phylogenetic diversity of the biofilm samples was performed by high throughput next generation sequencing (NGS). The analysis showed similar sets of mainly unidentified taxa of phyla Chloroflexi, Nitrospirae, Proteobacteria, Planctomycetes; however, large differences were found in their abundance. Cultivation-based method complemented with irradiation assay was performed using 5, 10 and 15 kGy doses of gamma-rays from a 60Co-source to reveal the extreme radiation-resistant bacteria. The phyla Actinobacteria, Firmicutes, Proteobacteria (classes Alpha- Beta- and Gammaproteobacteria), Bacteriodetes and Deinococcus-Thermus were represented among the 452 bacterial strains. The applied irradiation treatments promoted the isolation of 100 different species, involving candidate novel species, as well. The vast majority of the isolates belonged to bacterial taxa previously unknown as radiation-resistant microorganisms. Members of the genera Paracoccus, Marmoricola, Dermacoccus and Kytococcus were identified from the 15 kGy dose irradiated samples. The close relatives of several known radiation-tolerant bacteria were also detected from the biofilm samples, alongside with bacteria capable of detoxification by metal accumulation, adsorption and precipitation in the form of calcium-carbonate which possibly maintain the viability of the habitat. The results suggest the establishment of a unique, extremophilic microbiota in the studied hydrothermal spring caves. LA - English DB - MTMT ER - TY - THES AU - Reeda, Shawkat Harb TI - The inhibitory effect of the methanolic extracts of Origanum syriacum and Rosmarinus officinalis and their major components on the biofilm formation of clinical isolates of Staphylococcus aureus PY - 2019 UR - https://m2.mtmt.hu/api/publication/31324249 ID - 31324249 LA - English DB - MTMT ER - TY - GEN AU - González-Escobar, Jorge Luis AU - González-Purata, Perla Yanet AU - Veana-Hernández, Fabiola TI - ANÁLISIS DEL MICROBIOMA DEL MANANTIAL DE AGUAS TERMALES AZUFRADAS “TANINUL” DE LA HUASTECA POTOSINA PY - 2018 UR - https://m2.mtmt.hu/api/publication/31828993 ID - 31828993 LA - English DB - MTMT ER -