TY - JOUR AU - Antunes, B. AU - Zanchi, C. AU - Johnston, P.R. AU - Maron, B. AU - Witzany, C. AU - Regoes, R.R. AU - Hayouka, Z. AU - Rolff, J. TI - The evolution of antimicrobial peptide resistance in Pseudomonas aeruginosa is severely constrained by random peptide mixtures JF - PLOS BIOLOGY J2 - PLOS BIOL VL - 22 PY - 2024 IS - 7 July SN - 1544-9173 DO - 10.1371/journal.pbio.3002692 UR - https://m2.mtmt.hu/api/publication/35297196 ID - 35297196 AB - AU The:prevalence Pleaseconfirmthatallheadinglevelsarerepresentedcorrectly of antibiotic-resistant pathogens has become : a major threat to public health, requiring swift initiatives for discovering new strategies to control bacterial infections. Hence, antibiotic stewardship and rapid diagnostics, but also the development, and prudent use, of novel effective antimicrobial agents are paramount. Ideally, these agents should be less likely to select for resistance in pathogens than currently available conventional antimicrobials. The usage of antimicrobial peptides (AMPs), key components of the innate immune response, and combination therapies, have been proposed as strategies to diminish the emergence of resistance. Herein, we investigated whether newly developed random antimicrobial peptide mixtures (RPMs) can significantly reduce the risk of resistance evolution in vitro to that of single sequence AMPs, using the ESKAPE pathogen Pseudomonas aeruginosa (P. aeruginosa) as a model gram-negative bacterium. Infections of this pathogen are difficult to treat due the inherent resistance to many drug classes, enhanced by the capacity to form biofilms. P. aeruginosa was experimentally evolved in the presence of AMPs or RPMs, subsequentially assessing the extent of resistance evolution and cross-resistance/collateral sensitivity between treatments. Furthermore, the fitness costs of resistance on bacterial growth were studied and whole-genome sequencing used to investigate which mutations could be candidates for causing resistant phenotypes. Lastly, changes in the pharmacodynamics of the evolved bacterial strains were examined. Our findings suggest that using RPMs bears a much lower risk of resistance evolution compared to AMPs and mostly prevents cross-resistance development to other treatments, while maintaining (or even improving) drug sensitivity. This strengthens the case for using random cocktails of AMPs in favour of single AMPs, against which resistance evolved in vitro, providing an alternative to classic antibiotics worth pursuing. Copyright: © 2024 Antunes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. LA - English DB - MTMT ER - TY - JOUR AU - Asif, Fahim AU - Zaman, Sabbir Uz AU - Arnab, Md. Kamrul Hasan AU - Hasan, Moynul AU - Islam, Md. Monirul TI - Antimicrobial peptides as therapeutics: Confronting delivery challenges to optimize efficacy JF - The Microbe J2 - The Microbe VL - 2 PY - 2024 PG - 11 SN - 2950-1946 DO - 10.1016/j.microb.2024.100051 UR - https://m2.mtmt.hu/api/publication/35050295 ID - 35050295 LA - English DB - MTMT ER - TY - JOUR AU - Bao, Ruina AU - Ma, Zhi AU - Stanford, Kim AU - Mcallister, Tim A. AU - Niu, Yan D. TI - Antimicrobial Activities of α-Helix and β-Sheet Peptides against the Major Bovine Respiratory Disease Agent, Mannheimia haemolytica JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 8 SN - 1661-6596 DO - 10.3390/ijms25084164 UR - https://m2.mtmt.hu/api/publication/34837375 ID - 34837375 LA - English DB - MTMT ER - TY - JOUR AU - Barman, S. AU - Kurnaz, L.B. AU - Leighton, R. AU - Hossain, M.W. AU - Decho, A.W. AU - Tang, C. TI - Intrinsic antimicrobial resistance: Molecular biomaterials to combat microbial biofilms and bacterial persisters JF - BIOMATERIALS J2 - BIOMATERIALS VL - 311 PY - 2024 SN - 0142-9612 DO - 10.1016/j.biomaterials.2024.122690 UR - https://m2.mtmt.hu/api/publication/35297188 ID - 35297188 AB - The escalating rise in antimicrobial resistance (AMR) coupled with a declining arsenal of new antibiotics is imposing serious threats to global public health. A pervasive aspect of many acquired AMR infections is that the pathogenic microorganisms exist as biofilms, which are equipped with superior survival strategies. In addition, persistent and recalcitrant infections are seeded with bacterial persister cells at infection sites. Together, conventional antibiotic therapeutics often fail in the complete treatment of infections associated with bacterial persisters and biofilms. Novel therapeutics have been attempted to tackle AMR, biofilms, and persister-associated complex infections. This review focuses on the progress in designing molecular biomaterials and therapeutics to address acquired and intrinsic AMR, and the fundamental microbiology behind biofilms and persisters. Starting with a brief introduction of AMR basics and approaches to tackling acquired AMR, the emphasis is placed on various biomaterial approaches to combating intrinsic AMR, including (1) semi-synthetic antibiotics; (2) macromolecular or polymeric biomaterials mimicking antimicrobial peptides; (3) adjuvant effects in synergy; (4) nano-therapeutics; (5) nitric oxide-releasing antimicrobials; (6) antimicrobial hydrogels; (7) antimicrobial coatings. Particularly, the structure-activity relationship is elucidated in each category of these biomaterials. Finally, illuminating perspectives are provided for the future design of molecular biomaterials to bypass AMR and cure chronic multi-drug resistant (MDR) infections. © 2024 Elsevier Ltd LA - English DB - MTMT ER - TY - JOUR AU - Bhattacharjya, S. AU - Zhang, Z. AU - Ramamoorthy, A. TI - LL-37: Structures, Antimicrobial Activity, and Influence on Amyloid-Related Diseases JF - BIOMOLECULES J2 - BIOMOLECULES VL - 14 PY - 2024 IS - 3 SN - 2218-273X DO - 10.3390/biom14030320 UR - https://m2.mtmt.hu/api/publication/34784271 ID - 34784271 LA - English DB - MTMT ER - TY - JOUR AU - Chen, C. AU - Shi, J. AU - Wang, D. AU - Kong, P. AU - Wang, Z. AU - Liu, Y. TI - Antimicrobial peptides as promising antibiotic adjuvants to combat drug-resistant pathogens JF - CRITICAL REVIEWS IN MICROBIOLOGY J2 - CRIT REV MICROBIOL VL - 50 PY - 2024 IS - 3 SP - 267 EP - 284 PG - 18 SN - 1040-841X DO - 10.1080/1040841X.2023.2186215 UR - https://m2.mtmt.hu/api/publication/34886887 ID - 34886887 N1 - College of Veterinary Medicine, Yangzhou University, Yangzhou, China Institute of Comparative Medicine, Yangzhou University, Yangzhou, China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, China Cited By :5 Export Date: 16 September 2024 CODEN: CRVMA Correspondence Address: Wang, Z.; College of Veterinary Medicine, China; email: zqwang@yzu.edu.cn Correspondence Address: Liu, Y.; College of Veterinary Medicine, China; email: liuyuan2018@yzu.edu.cn Chemicals/CAS: Anti-Bacterial Agents; Antimicrobial Peptides LA - English DB - MTMT ER - TY - JOUR AU - Chen, J. AU - Wang, W. AU - Hu, X. AU - Yue, Y. AU - Lu, X. AU - Wang, C. AU - Wei, B. AU - Zhang, H. AU - Wang, H. TI - Medium-sized peptides from microbial sources with potential for antibacterial drug development JF - NATURAL PRODUCT REPORTS J2 - NAT PROD REP VL - 41 PY - 2024 IS - 8 SP - 1235 EP - 1263 PG - 29 SN - 0265-0568 DO - 10.1039/d4np00002a UR - https://m2.mtmt.hu/api/publication/35232364 ID - 35232364 LA - English DB - MTMT ER - TY - CHAP AU - Cifuentes, J. AU - Ruiz, Puentes P. AU - Arbeláez, P. AU - Muñoz-Camargo, C. AU - Cruz, J.C. AU - Reyes, L.H. TI - Antimicrobial activities of casein T2 - Casein PB - Elsevier SN - 9780443158360 PY - 2024 SP - 331 EP - 353 PG - 23 DO - 10.1016/B978-0-443-15836-0.00001-9 UR - https://m2.mtmt.hu/api/publication/35297207 ID - 35297207 AB - In the past decade, the rise of multidrug-resistant bacterial strains has limited the use of conventional antibiotics in the pharmaceutical and food industries, thereby exposing a significant worldwide health concern. On account of this, antimicrobial peptides (AMPs) are documented as broad-spectrum antimicrobials and have attracted particular interest as prospective alternatives to conventional pharmaceutical formulations and food preservatives. Casein, the most prevalent protein family in milk, has been cited as an indispensable source of bioactive peptides. In consequence, this chapter describes the various in vitro and in silico techniques used to extract and validate casein-derived AMPs. In addition, this chapter provides a detailed analysis of all previously described casein-derived AMPs, hence allowing the identification of the key AMP sources and the corresponding production and release mechanisms and strategies. Finally, solutions based on deep learning are proposed as viable and effective alternatives to the current AMPs-discovery methodologies. © 2024 by Elsevier Inc. All rights reserved, including those for text and data mining, AI training, and similar technologies. LA - English DB - MTMT ER - TY - JOUR AU - Deng, H. AU - Zhang, S. AU - Fu, Y. AU - Dong, N. AU - Bi, C. AU - Shan, A. AU - Shao, C. TI - Advances in the delivery and application of antimicrobial peptide-based nanomaterials JF - CHEMICAL ENGINEERING JOURNAL J2 - CHEM ENG J VL - 496 PY - 2024 SN - 1385-8947 DO - 10.1016/j.cej.2024.154232 UR - https://m2.mtmt.hu/api/publication/35297190 ID - 35297190 AB - As multidrug-resistant bacteria continue to emerge, antimicrobial peptides (AMPs) are being explored as alternatives to antibiotics due to their distinct action mechanism that appears to be devoid of resistance. However, unbound AMPs have a short life span in circulation, are sensitive to proteases, and are unable to withstand alkaline wound environments. In contrast, AMP-based nanomaterials exhibit the ability to overcome these obstacles, resulting in superior therapeutic efficacy compared to free AMPs across disease models. This review focuses on the rational methods used for fabricating peptide-based nanostructures and provides detailed descriptions of their potential in treating microbial infections in different parts of the body, such as the bloodstream, pulmonary system, chronic wounds, gastrointestinal tract, and meningitis. The challenges that impede their oral administration are also discussed. Altogether, this article encapsulates recent advancements and prospects in the formulation and application of AMP-based nanomaterials, so as to provide references for the development of polypeptide biomaterials. © 2024 Elsevier B.V. LA - English DB - MTMT ER - TY - JOUR AU - Fathi, Fariba AU - Alizadeh, Bahareh AU - Tabarzad, Mohammad Vahid AU - Tabarzad, Maryam TI - Important structural features of antimicrobial peptides towards specific activity: Trends in the development of efficient therapeutics JF - BIOORGANIC CHEMISTRY J2 - BIOORG CHEM VL - 149 PY - 2024 PG - 18 SN - 0045-2068 DO - 10.1016/j.bioorg.2024.107524 UR - https://m2.mtmt.hu/api/publication/35202795 ID - 35202795 N1 - Funding Agency and Grant Number: Shahid Beheshti University of Medical Sciences, Tehran, Iran [43003231] Funding text: This manuscript was financially supported by Shahid Beheshti University of Medical Sciences, Tehran, Iran (Grant# 43003231). AB - Proteins and peptides, as polypeptide chains, have usually got unique conformational structures for effective biological activity. Antimicrobial peptides (AMPs) are a group of bioactive peptides, which have been increasingly studied during recent years for their promising antibacterial, antifungal, antiviral and anti-inflammatory activity, as well as, other esteemed bioactivities. Numerous AMPs have been separated from a wide range of natural resources, or produced in vitro through chemical synthesis and recombinant protein expression. Natural AMPs have had limited clinical application due to several drawbacks, such as their short half-life due to protease degradation, lack of activity at physiological salt concentrations, toxicity to mammalian cells, and the absence of suitable methods of delivery for the AMPs that are targeted and sustained. The creation of synthetic analogs of AMPs would both avoid the drawbacks of the natural analogs and maintain or even increase the antimicrobial effectiveness. The structure-activity relationship of discovered AMPs or their derivatives facilitates the development of synthetic AMPs. This review discovered that the relationship between the activity of AMPs and their positive net charge, hydrophobicity, and amino acid sequence and the relationship between AMPs' function and other features like their topology, glycosylation, and halogenation. LA - English DB - MTMT ER - TY - JOUR AU - Gong, Haoning AU - Wang, Xiaonan AU - Hu, Xuzhi AU - Liao, Mingrui AU - Yuan, Chengqian AU - Lu, Jian Ren AU - Gao, Lizeng AU - Yan, Xuehai TI - Effective Treatment of Helicobacter pylori Infection Using Supramolecular Antimicrobial Peptide Hydrogels JF - BIOMACROMOLECULES J2 - BIOMACROMOLECULES VL - 25 PY - 2024 IS - 3 SP - 1602 EP - 1611 PG - 10 SN - 1525-7797 DO - 10.1021/acs.biomac.3c01141 UR - https://m2.mtmt.hu/api/publication/34837379 ID - 34837379 LA - English DB - MTMT ER - TY - JOUR AU - Huo, Xingchen AU - Wang, Pengxu AU - Zhao, Fengxia AU - Liu, Qian AU - Yang, Chunrong AU - Zhang, Yongan AU - Su, Jianguo TI - High-efficiency expression of a novel antimicrobial peptide I20 with superior bactericidal ability and biocompatibility in Pichia pastoris and its efficiency enhancement to aquaculture JF - AQUACULTURE J2 - AQUACULTURE VL - 579 PY - 2024 PG - 15 SN - 0044-8486 DO - 10.1016/j.aquaculture.2023.740149 UR - https://m2.mtmt.hu/api/publication/34312618 ID - 34312618 N1 - Export Date: 28 November 2023; CODEN: AQCLA AB - One of the major constraints on aquaculture is the susceptibility of farmed fish to diseases or slow growing. Antimicrobial peptide I20, as a novel feed additive, is used to cope with these limiting factors. In the current study, the Pichia pastoris expression system was used to produce I20 in large quantities, thereby reducing its application cost. In terms of molecular strategy, the I20 expression codon was optimized according to the preference of P. pastoris codon. Through traditional strategy optimization (expression strain, time, methanol concentration, temperature, and pH), the expression level of I20 in shaking bottles was 21.09 +/- 1.10 mg/L. Surprisingly, the expression level of I20 in a 20 L high-cell-density cultivation was 514.78 +/- 22.07 mg/L after methanol induction of 72 h. Comprehensive analyses using a series of fluorescence and microscopy revealed that I20 killed bacteria by increasing bacterial membrane permeability and binding bacterial DNA. I20 effectively killed a variety of pathogenic bacteria (Aeromonas hydrophila, Aeromonas veronii, Elizabethkingia miricola, Fla-vobacterium columnare, Edwardsiella ictaluri, Vibrio parahaemolyticus, Streptococcus agalactiae, Nocardia seriolae, Escherichia coli, and Staphylococcus aureus). I20 was found to have excellent biocompatibility after both feeding and injection studies. Grass carp were fed I20 diet supplemented with 200 mg/Kg I20 for 8 weeks. I20 can effectively promote intestinal villus length, secretion of digestive enzymes, and expression of growth genes to improve growth performance. I20 significantly promotes immune response to reduce tissue damage and bacterial load, enhancing survival after Aeromonas hydrophila infection. In conclusion, we systematically optimized the expression of antimicrobial peptide I20 in P. pastoris, and achieved a satisfactory efficiency for producing antimicrobial peptide I20 by integrating several strategies. The results herein showed that I20-feed promoted growth performance and disease resistance in fish, suggesting a feed supplement with great potential applica-tions for aquaculture. LA - English DB - MTMT ER - TY - JOUR AU - Kwon, Ryan S. AU - Lee, Gi Young AU - Lee, Sohyoung AU - Song, Jeongmin TI - Antimicrobial properties of tomato juice and peptides against typhoidal Salmonella JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 12 PY - 2024 IS - 3 PG - 14 SN - 2165-0497 DO - 10.1128/spectrum.03102-23 UR - https://m2.mtmt.hu/api/publication/34628118 ID - 34628118 N1 - Export Date: 4 June 2024 Correspondence Address: Song, J.; Department of Microbiology and Immunology, United States; email: jeongmin.song@cornell.edu LA - English DB - MTMT ER - TY - JOUR AU - Laborda, Pablo AU - Gil‐Gil, Teresa AU - Martínez, José Luis AU - Hernando‐Amado, Sara TI - Preserving the efficacy of antibiotics to tackle antibiotic resistance JF - MICROBIAL BIOTECHNOLOGY J2 - MICROB BIOTECHNOL VL - 17 PY - 2024 IS - 7 SN - 1751-7907 DO - 10.1111/1751-7915.14528 UR - https://m2.mtmt.hu/api/publication/35151331 ID - 35151331 AB - Different international agencies recognize that antibiotic resistance is one of the most severe human health problems that humankind is facing. Traditionally, the introduction of new antibiotics solved this problem but various scientific and economic reasons have led to a shortage of novel antibiotics at the pipeline. This situation makes mandatory the implementation of approaches to preserve the efficacy of current antibiotics. The concept is not novel, but the only action taken for such preservation had been the ‘prudent’ use of antibiotics, trying to reduce the selection pressure by reducing the amount of antibiotics. However, even if antibiotics are used only when needed, this will be insufficient because resistance is the inescapable outcome of antibiotics' use. A deeper understanding of the alterations in the bacterial physiology upon acquisition of resistance and during infection will help to design improved strategies to treat bacterial infections. In this article, we discuss the interconnection between antibiotic resistance (and antibiotic activity) and bacterial metabolism, particularly in vivo, when bacteria are causing infection. We discuss as well how understanding evolutionary trade‐offs, as collateral sensitivity, associated with the acquisition of resistance may help to define evolution‐based therapeutic strategies to fight antibiotic resistance and to preserve currently used antibiotics. LA - English DB - MTMT ER - TY - JOUR AU - Liang, Y. AU - Zhang, Y. AU - Huang, Y. AU - Xu, C. AU - Chen, J. AU - Zhang, X. AU - Huang, B. AU - Gan, Z. AU - Dong, X. AU - Huang, S. AU - Li, C. AU - Jia, S. AU - Zhang, P. AU - Yuan, Y. AU - Zhang, H. AU - Wang, Y. AU - Yuan, B. AU - Bao, Y. AU - Xiao, S. AU - Xiong, M. TI - Helicity-directed recognition of bacterial phospholipid via radially amphiphilic antimicrobial peptides JF - SCIENCE ADVANCES J2 - SCI ADV VL - 10 PY - 2024 IS - 35 SN - 2375-2548 DO - 10.1126/sciadv.adn9435 UR - https://m2.mtmt.hu/api/publication/35297193 ID - 35297193 AB - The fundamental differences in phospholipids between bacterial and mammalian cell membranes present remarkable opportunities for antimicrobial design. However, it is challenging to distinguish bacterial anionic phospholipid phosphatidylglycerol (PG) from mammalian anionic phosphatidylserine (PS) with the same net charge. Here, we report a class of radially amphiphilic α helix antimicrobial peptides (RAPs) that can selectively discriminate PG from PS, relying on the helix structure. The representative RAP, L10-MMBen, can direct the rearrangement of PG vesicles into a lamellar structure with its helix axis parallel to the PG membrane surface. The helical structure imparts both the thermodynamic and kinetic advantages of L10-MMBen/PG assembly, and the hiding of hydrophobic regions in RAPs is crucial for PG recognition. L10-MMBen exhibits high selectivity against bacteria depending on PG recognition, showing low in vivo toxicity and significant treatment efficacy in mice infection models. Our study introduces a helicity-direct bacterial phospholipid recognition paradigm for designing highly selective antimicrobial peptides. Copyright © 2024 The Authors, some rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Lim, Jeonghyun AU - Myung, Heejoon AU - Lim, Daejin AU - Song, Miryoung TI - Antimicrobial peptide thanatin fused endolysin PA90 (Tha-PA90) for the control of Acinetobacter baumannii infection in mouse model JF - JOURNAL OF BIOMEDICAL SCIENCE J2 - J BIOMED SCI VL - 31 PY - 2024 IS - 1 SN - 1021-7770 DO - 10.1186/s12929-024-01027-4 UR - https://m2.mtmt.hu/api/publication/34837374 ID - 34837374 LA - English DB - MTMT ER - TY - JOUR AU - Mahdavi, Michael AU - Findlay, Brandon L. TI - Discovery of an adjuvant that resensitizes polymyxin B-resistant bacteria JF - BIOORGANIC & MEDICINAL CHEMISTRY J2 - BIOORGAN MED CHEM VL - 97 PY - 2024 SN - 0968-0896 DO - 10.1016/j.bmc.2023.117541 UR - https://m2.mtmt.hu/api/publication/34524605 ID - 34524605 LA - English DB - MTMT ER - TY - CHAP AU - Mahmoud, G.A.-E. AU - Riad, O.K.M. AU - Badawy, M.S.E.M. ED - Srivastava, A. ED - Department, of Microbiology Banaras Hindu University Varanasi ED - Mishra, V. ED - Amity, Institute of Microbial Technology Amity University Uttar Pradesh, Noida TI - Production and development of novel drug targets through AI T2 - Methods in Microbiology VL - 55 PB - Academic Press Inc. SN - 9780443296246 T3 - Methods in Microbiology, ISSN 0580-9517 ; 55. PY - 2024 SP - 1 EP - 28 PG - 28 DO - 10.1016/bs.mim.2024.05.002 UR - https://m2.mtmt.hu/api/publication/35234273 ID - 35234273 LA - English DB - MTMT ER - TY - JOUR AU - Medvedeva, A. AU - Teimouri, H. AU - Kolomeisky, A.B. TI - Differences in Relevant Physicochemical Properties Correlate with Synergistic Activity of Antimicrobial Peptides JF - JOURNAL OF PHYSICAL CHEMISTRY B J2 - J PHYS CHEM B VL - 128 PY - 2024 IS - 6 SP - 1407 EP - 1417 PG - 11 SN - 1520-6106 DO - 10.1021/acs.jpcb.3c07663 UR - https://m2.mtmt.hu/api/publication/34799284 ID - 34799284 N1 - Export Date: 19 April 2024; CODEN: JPCBF LA - English DB - MTMT ER - TY - JOUR AU - Muñoz-Camargo, C. AU - Cruz, J.C. TI - From inside to outside: exploring extracellular antimicrobial histone-derived peptides as multi-talented molecules JF - JOURNAL OF ANTIBIOTICS J2 - J ANTIBIOT VL - 77 PY - 2024 IS - 9 SP - 553 EP - 568 PG - 16 SN - 0021-8820 DO - 10.1038/s41429-024-00744-0 UR - https://m2.mtmt.hu/api/publication/35297191 ID - 35297191 AB - The emergence of bacterial resistance to antibiotics poses a global health threat, necessitating innovative solutions. The contemporary challenge lies in bacterial resistance, impacting morbidity, mortality, and global economies. Antimicrobial peptides (AMPs) offer a promising avenue for addressing antibiotic resistance. The Antimicrobial Peptide Database catalogs 3569 peptides from various organisms, representing a rich resource for drug development. Histones, traditionally recognized for their role in nucleosome structures, have gained attention for their extracellular functions, including antimicrobial and immunomodulatory properties. This review aims to thoroughly investigate antimicrobial peptides derived from histones in various organisms, elucidating their mechanisms. In addition, it gives us clues about how extracellular histones might be used in drug delivery systems to fight bacterial infections. This comprehensive analysis emphasizes the importance of histone-derived peptides in developing innovative therapeutic strategies for evolving bacterial challenges. © The Author(s) 2024. LA - English DB - MTMT ER - TY - JOUR AU - Rad, P.M. AU - Rahbarnia, L. AU - Safary, A. AU - ShadiDizaji, A. AU - Maani, Z. TI - The Synthetic Antimicrobial Peptide Derived From Melittin Displays Low Toxicity and Anti-infectious Properties JF - PROBIOTICS AND ANTIMICROBIAL PROTEINS J2 - PROBIOTICS ANTIMICROB PROTEINS VL - 16 PY - 2024 IS - 2 SP - 490 EP - 500 PG - 11 SN - 1867-1306 DO - 10.1007/s12602-023-10066-6 UR - https://m2.mtmt.hu/api/publication/34837515 ID - 34837515 LA - English DB - MTMT ER - TY - JOUR AU - Raza, Muhammad Zain AU - Arshad, Hafiz Muhammad Ehsan AU - Maqsood, Musab AU - Faisal, Muhammad Hashim TI - The mechanisms and clinical potential of collateral sensitivity in Mycobacterium tuberculosis: A literature review JF - The Microbe J2 - The Microbe VL - 3 PY - 2024 SN - 2950-1946 DO - 10.1016/j.microb.2024.100086 UR - https://m2.mtmt.hu/api/publication/34897426 ID - 34897426 LA - English DB - MTMT ER - TY - JOUR AU - Shabani, Sadegh AU - Hadjigol, Sara AU - Li, Wenyi AU - Si, Zhangyong AU - Pranantyo, Dicky AU - Chan-Park, Mary B. AU - O’Brien-Simpson, Neil M. AU - Qiao, Greg G. TI - Synthetic peptide branched polymers for antibacterial and biomedical applications JF - Nature Reviews Bioengineering J2 - Nat Rev Bioeng VL - 2 PY - 2024 IS - 4 SP - 343 EP - 361 PG - 19 SN - 2731-6092 DO - 10.1038/s44222-023-00143-4 UR - https://m2.mtmt.hu/api/publication/35050300 ID - 35050300 LA - English DB - MTMT ER - TY - JOUR AU - Shang, Lu AU - Chen, Chan AU - Sun, Rui AU - Guo, Juan AU - Liu, Jing AU - Wang, Mi AU - Zhang, Lifang AU - Fei, Chenzhong AU - Xue, Feiqun AU - Liu, Yingchun AU - Gu, Feng TI - Engineered Peptides Harboring Cation Motifs Against Multidrug-Resistant Bacteria JF - ACS APPLIED MATERIALS & INTERFACES J2 - ACS APPL MATER INTER VL - 16 PY - 2024 IS - 5 SP - 5522 EP - 5535 PG - 14 SN - 1944-8244 DO - 10.1021/acsami.3c15913 UR - https://m2.mtmt.hu/api/publication/34628115 ID - 34628115 N1 - Export Date: 28 February 2024 LA - English DB - MTMT ER - TY - JOUR AU - Shi, Qianyu AU - Chen, Jibing AU - Chen, Junsheng AU - Liu, Yanfeng AU - Wang, Hongze TI - Application of additively manufactured bone scaffold: a systematic review JF - BIOFABRICATION J2 - BIOFABRICATION VL - 16 PY - 2024 IS - 2 SN - 1758-5082 DO - 10.1088/1758-5090/ad35e8 UR - https://m2.mtmt.hu/api/publication/34837376 ID - 34837376 LA - English DB - MTMT ER - TY - JOUR AU - Smith, Brooke L. AU - Fernando, Sandun AU - King, Maria D. TI - Escherichia coli resistance mechanism AcrAB-TolC efflux pump interactions with commonly used antibiotics: a molecular dynamics study JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-024-52536-z UR - https://m2.mtmt.hu/api/publication/34628116 ID - 34628116 N1 - Export Date: 28 February 2024 LA - English DB - MTMT ER - TY - JOUR AU - Stepulane, A. AU - Rajasekharan, A.K. AU - Andersson, M. TI - Antibacterial efficacy of antimicrobial peptide-functionalized hydrogel particles combined with vancomycin and oxacillin antibiotics JF - INTERNATIONAL JOURNAL OF PHARMACEUTICS J2 - INT J PHARM VL - 664 PY - 2024 SN - 0378-5173 DO - 10.1016/j.ijpharm.2024.124630 UR - https://m2.mtmt.hu/api/publication/35297189 ID - 35297189 AB - The rise of antibiotic resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), requires novel approaches to combat infections. Medical devices like implants and wound dressings are frequently used in conjunction with antibiotics, motivating the development of antibacterial biomaterials capable of exhibiting combined antibacterial effects with conventional antibiotics. This study explores the synergistic antibacterial effects of combining antimicrobial peptide (AMP) functionalized hydrogel particles with conventional antibiotics, vancomycin (VCM) and oxacillin (OXA), against Staphylococcus aureus and MRSA. The AMP employed, RRPRPRPRPWWWW-NH2, has previously demonstrated broad-spectrum activity and enhanced stability when attached to hydrogel substrates. Here, checkerboard assays revealed additive and synergistic interactions between the free AMP and both VCM and OXA against Staphylococcus aureus and MRSA. Notably, the AMP-OXA combination displayed a significant synergistic effect against MRSA, with a 512-fold reduction in OXA's minimum inhibitory concentration (MIC) when combined with free AMP. The observed synergism against MRSA was retained upon covalent AMP immobilization onto the hydrogel particles; however, at a lower rate with a 64-fold reduction in OXA MIC. Despite this, the OXA-AMP hydrogel particle combinations retained considerable synergistic potential against MRSA, a strain resistant to OXA, highlighting the potential of AMP-functionalized materials for enhancing antibiotic efficacy. These findings underscore the importance of developing antimicrobial biomaterials for future medical devices to fight biomaterial-associated infections and reverse antimicrobial resistance. © 2024 The Authors LA - English DB - MTMT ER - TY - JOUR AU - Thakur, A. AU - Maroju, P.A. AU - Ganesan, R. AU - Ray, Dutta J. TI - Contrasting responses of motile and non-motile Escherichia coli strains in resuscitation against stable ultrafine gold nanosystems JF - MICRO AND NANO SYSTEMS LETTERS J2 - MICRO NANO SYST LETT VL - 12 PY - 2024 IS - 1 SN - 2213-9621 DO - 10.1186/s40486-024-00206-0 UR - https://m2.mtmt.hu/api/publication/35297186 ID - 35297186 AB - Global public health confronts a pressing challenge in antimicrobial resistance (AMR), necessitating urgent intervention strategies due to the low success rate of new antibiotic development. Bacterial motility, beyond conventional antibiotic usage, significantly influences resistance evolution and ecological dynamics. Our recent study marks a breakthrough, revealing the unexplored ability of ultrafine gold nanosystems (UGNs) to inhibit bacterial resuscitation using a motile Escherichia coli (E.coli) K12 strain. We aim to deepen our comparative understanding of UGNs’ efficacy and resuscitation propensity against a non-motile E. coli K12 strain to assess the role of motility. Through UGN application, we identified heritable resistance in both strains, with motile strains exhibiting notably higher mutation rates. Resuscitation experiments unveiled faster recovery in motile strains, attributable to virulence factors, compared to non-motile strains. Additionally, our investigation into aggregation dynamics highlighted the role of protein-mediated aggregation in resistance development to nano-antimicrobials. Overall, the study reveals that the non-motile strains are more susceptible against UGNs, which shows promise in combating AMR. © The Author(s) 2024. LA - English DB - MTMT ER - TY - JOUR AU - Wang, H. AU - Yang, Y. AU - Wang, S. AU - Badawy, S. AU - Ares, I. AU - Martínez, M. AU - Lopez-Torres, B. AU - Martínez-Larrañaga, M.-R. AU - Wang, X. AU - Anadón, A. AU - Martínez, M.-A. TI - Antimicrobial sensitisers: Gatekeepers to avoid the development of multidrug-resistant bacteria JF - JOURNAL OF CONTROLLED RELEASE J2 - J CONTROL RELEASE VL - 369 PY - 2024 SP - 25 EP - 38 PG - 14 SN - 0168-3659 DO - 10.1016/j.jconrel.2024.03.031 UR - https://m2.mtmt.hu/api/publication/34766195 ID - 34766195 N1 - National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Hubei, Wuhan, 430070, China MAO Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Hubei, Wuhan, 430070, China Pathology Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University, Egypt Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Universidad Complutense de Madrid (UCM), and Research Institute Hospital, 12 de Octubre (i+12), Madrid, 28040, Spain Export Date: 3 April 2024 CODEN: JCREE Correspondence Address: Anadón, A.; Department of Pharmacology and Toxicology, Spain; email: aanadon@ucm.es Correspondence Address: Wang, X.; National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Hubei, China; email: wangxu@mail.hzau.edu.cn AB - The resistance of multidrug-resistant bacteria to existing antibiotics forces the continued development of new antibiotics and antibacterial agents, but the high costs and long timeframe involved in the development of new agents renders the hope that existing antibiotics may again play a part. The “antibiotic adjuvant” is an indirect antibacterial strategy, but its vague concept has, in the past, limited the development speed of related drugs. In this review article, we put forward an accurate concept of a “non-self-antimicrobial sensitisers (NSAS)”, to distinguish it from an “antibiotic adjuvant”, and then discuss several scientific methods to restore bacterial sensitivity to antibiotics, and the sources and action mechanism of existing NSAS, in order to guide the development and further research of NSAS. © 2024 The Author(s) LA - English DB - MTMT ER - TY - JOUR AU - Wu, J. AU - Yan, J. AU - Xu, S. AU - Zou, X. AU - Xu, Y. AU - Jin, X. AU - Lu, X. AU - Gui, S. TI - Novel Nano Drug-Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI JF - ADVANCED HEALTHCARE MATERIALS J2 - ADV HEALTHC MATER PY - 2024 SN - 2192-2640 DO - 10.1002/adhm.202401745 UR - https://m2.mtmt.hu/api/publication/35297208 ID - 35297208 AB - Catheter-associated urinary tract infection (CAUTI) is a prevalent type of hospital-acquired infection, affecting approximately 15% to 25% of patients with urinary catheters. Long-term use of the catheter can lead to colonization of microorganisms and biofilm formation, and may develop into bacterial CAUTI. However, the frequent replacement of catheters in clinical settings can result in tissue damage, inflammation, ulceration, and additional complications, causing discomfort and pain for patients. In light of these challenges, a novel nanodrug-supported hydrogel coating called NP-AM/FK@OMV-P/H has been developed in this study. Through in vitro experiments, it is confirmed that OMV nano-loaded liquid gel coating has an effective reaction against E.coli HAase and releases antibacterial drugs. This coating has also demonstrated strong inhibition of E.coli and has shown the ability to inhibit the formation of bacterial biofilm. These findings highlight the potential of the OMV nanoparticle gel coating in preventing and treating bacterial infections. Notably, NP-AM/FK@OMV-P/H has exhibited greater efficacy against multidrug-resistant E.coli associated with UTIs compared to coatings containing single antimicrobial peptides or antibiotics. Additionally, it has demonstrated good biosecurity. In conclusion, the NP-AM/FK@OMV-P/H coating holds great potential in providing benefits to patients with CAUTI. © 2024 The Author(s). Advanced Healthcare Materials published by Wiley-VCH GmbH. LA - English DB - MTMT ER - TY - JOUR AU - Zhao, Chongyi AU - Yan, Shuo AU - Luo, Ying AU - Song, Yuzhu AU - Xia, Xueshan TI - Analyzing resistome in soil and Human gut: a study on the characterization and risk evaluation of antimicrobial peptide resistance JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 15 PY - 2024 PG - 11 SN - 1664-302X DO - 10.3389/fmicb.2024.1352531 UR - https://m2.mtmt.hu/api/publication/34769325 ID - 34769325 N1 - Export Date: 16 September 2024 Correspondence Address: Song, Y.; Faculty of Life Science and Technology, China; email: yuzhusong@kmust.edu.cn Correspondence Address: Xia, X.; Faculty of Life Science and Technology, China; email: oliverxia2000@aliyun.com Chemicals/CAS: ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; cathelicidin, 170006-50-5; cephalosporin, 11111-12-9; colistin, 1066-17-7, 1264-72-8; iseganan, 257277-05-7, 244015-05-2, 256475-21-5; melittin, 20449-79-0, 37231-28-0, 65742-02-1; polymyxin B, 1404-26-8, 1405-20-5; sodium dihydrogen phosphate, 7558-80-7, 7632-05-5 Tradenames: HiSeq, Illumina; NovaSeq 6000, Illumina; QIAamp, Qiagen Manufacturers: Dangang; SolarbioIllumina; Qiagen Funding details: 2022202AG050013 Funding details: 2022FKZDZK-07 Funding text 1: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by Yunnan Major Scientific and Technological Projects (No. 2022202AG050013); the Key Specialist Project of Gynecology in Yunnan Province (No. 2022FKZDZK-07). LA - English DB - MTMT ER - TY - JOUR AU - Abbasi, Pezhman AU - Moghadam, Ehsan Bahrami TI - Electrochemical degradation of ciprofloxacin from water: Modeling and prediction using ANN and LSSVM JF - PHYSICS AND CHEMISTRY OF THE EARTH (2002-) J2 - PHYS CHEM EARTH (2002-) VL - 132 PY - 2023 PG - 10 SN - 1474-7065 DO - 10.1016/j.pce.2023.103509 UR - https://m2.mtmt.hu/api/publication/34628119 ID - 34628119 N1 - Export Date: 28 February 2024; CODEN: PCEHA LA - English DB - MTMT ER - TY - JOUR AU - Anurag, Anand A. AU - Amod, A. AU - Anwar, S. AU - Sahoo, A.K. AU - Sethi, G. AU - Samanta, S.K. TI - A comprehensive guide on screening and selection of a suitable AMP against biofilm-forming bacteria JF - CRITICAL REVIEWS IN MICROBIOLOGY J2 - CRIT REV MICROBIOL PY - 2023 SN - 1040-841X DO - 10.1080/1040841X.2023.2293019 UR - https://m2.mtmt.hu/api/publication/34523041 ID - 34523041 AB - Lately, antimicrobial resistance (AMR) is increasing at an exponential rate making it important to search alternatives to antibiotics in order to combat multi-drug resistant (MDR) bacterial infections. Out of the several antibacterial and antibiofilm strategies being tested, antimicrobial peptides (AMPs) have shown to give better hopes in terms of a long-lasting solution to the problem. To select a desired AMP, it is important to make right use of available tools and databases that aid in identification, classification, and analysis of the physiochemical properties of AMPs. To identify the targets of these AMPs, it becomes crucial to understand their mode-of-action. AMPs can also be used in combination with other antibacterial and antibiofilm agents so as to achieve enhanced efficacy against bacteria and their biofilms. Due to concerns regarding toxicity, stability, and bioavailability, strategizing drug formulation at an early-stage becomes crucial. Although there are few concerns regarding development of bacterial resistance to AMPs, the evolution of resistance to AMPs occurs extremely slowly. This comprehensive review gives a deep insight into the selection of the right AMP, deciding the right target and combination strategy along with the type of formulation needed, and the possible resistance that bacteria can develop to these AMPs. © 2023 Informa UK Limited, trading as Taylor & Francis Group. LA - English DB - MTMT ER - TY - JOUR AU - Babazadeh, Daryoush AU - Abd, El-Ghany Wafaa TI - Distribution, Infection, Diagnosis, and Control of Avibacterium paragallinarum in Poultry JF - KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI J2 - KAFKAS UNIV VET FAK VL - 29 PY - 2023 IS - 6 SP - 595 EP - 609 PG - 15 SN - 1300-6045 DO - 10.9775/kvfd.2023.30320 UR - https://m2.mtmt.hu/api/publication/34837391 ID - 34837391 LA - English DB - MTMT ER - TY - JOUR AU - Bortolotti, A. AU - Troiano, C. AU - Bobone, S. AU - Konai, M. M. AU - Ghosh, C. AU - Bocchinfuso, G. AU - Acharya, Y. AU - Santucci, V. AU - Bonacorsi, S. AU - Di, Stefano C. AU - Haldar, J. AU - Stella, L. TI - Mechanism of lipid bilayer perturbation by bactericidal membrane-active small molecules JF - BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES J2 - BBA-BIOMEMBRANES VL - 1865 PY - 2023 IS - 1 PG - 11 SN - 0005-2736 DO - 10.1016/j.bbamem.2022.184079 UR - https://m2.mtmt.hu/api/publication/34770447 ID - 34770447 N1 - Funding Agency and Grant Number: Italian Ministry of University and Research [PRIN 2020833Y75]; Indo-French Centrefor the Promotion of Advanced Research (IFCPAR/CEFIPRA) [IFC/62T10-1/2019/764] Funding text: This work was supported by the Italian Ministry of University and Research (grant PRIN 2020833Y75, to LS) and the Indo-French Centrefor the Promotion of Advanced Research (IFCPAR/CEFIPRA, project IFC/62T10-1/2019/764, to JH). GB would like to thank the CINECA consortium for providing high performance computational resources. AB - Membrane-active small molecules (MASMs) are small organic molecules designed to reproduce the fundamental physicochemical properties of natural antimicrobial peptides: their cationic charge and amphiphilic character. This class of compounds has a promising broad range of antimicrobial activity and, at the same time, solves some major limitations of the peptides, such as their high production costs and low in vivo stability. Most cationic antimicrobial peptides act by accumulating on the surface of bacterial membranes and causing the formation of defects when a threshold is reached. Due to the drastically different structures of the two classes of molecules, it is not obvious that small-molecule antimicrobials act in the same way as natural peptides, and very few data are available on this aspect. Here we combined spectroscopic studies and molecular dynamics simulations to characterize the mechanism of action of two different MASMs. Our results show that, notwithstanding their simple structure, these molecules act just like antimicrobial peptides. They bind to the membrane surface, below the head-groups, and insert their apolar moieties in the core of the bilayer. Like many natural peptides, they cause the formation of defects when they reach a high coverage of the membrane surface. In addition, they cause membrane aggregation, and this property could contribute to their antimicrobial activity. LA - English DB - MTMT ER - TY - JOUR AU - Carrozza, D. AU - Malavasi, G. AU - Ferrari, E. TI - Very Large Pores Mesoporous Silica as New Candidate for Delivery of Big Therapeutics Molecules, Such as Pharmaceutical Peptides JF - MATERIALS J2 - MATERIALS VL - 16 PY - 2023 IS - 11 SN - 1996-1944 DO - 10.3390/ma16114151 UR - https://m2.mtmt.hu/api/publication/34025037 ID - 34025037 N1 - Export Date: 20 June 2023 Correspondence Address: Malavasi, G.; Department of Chemical and Geological Sciences, Via G. Campi 103, Italy; email: gianluca.malavasi@unimore.it AB - The synthesis of a scaffold that can accommodate big molecules with a pharmaceutical role is important to shield them and maintain their biological activity. In this field, silica particles with large pores (LPMS) are innovative supports. Large pores allow for the loading of bioactive molecules inside the structure and contemporarily their stabilization and protection. These purposes cannot be achieved using classical mesoporous silica (MS, pore size 2–5 nm), because their pores are not big enough and pore blocking occurs. LPMSs with different porous structures are synthesized starting from an acidic water solution of tetraethyl orthosilicate reacting with pore agents (Pluronic® F127 and mesitylene), performing hydrothermal and microwave-assisted reactions. Time and surfactant optimization were performed. Loading tests were conducted using Nisin as a reference molecule (polycyclic antibacterial peptide, with dimensions of 4–6 nm); UV-Vis analyses on loading solutions were performed. For LPMSs, a significantly higher loading efficiency (LE%) was registered. Other analyses (Elemental Analysis, Thermogravimetric Analysis and UV-Vis) confirmed the presence of Nisin in all the structures and its stability when loaded on them. LPMSs showed a lower decrease in specific surface area if compared to MS; in terms of the difference in LE% between samples, it is explained considering the filling of pores for LPMSs, a phenomenon that is not allowed for MSs. Release studies in simulated body fluid highlight, only for LPMSs, a controlled release, considering the longer time scale of release. Scanning Electron Microscopy images acquired before and after release tests shows the LPMSs’ maintenance of the structure, demonstrating strength and mechanical resistance of structures. In conclusion, LPMSs were synthesized, performing time and surfactant optimization. LPMSs showed better loading and releasing properties with respect to classical MS. All collected data confirm a pore blocking for MS and an in-pore loading for LPMS. © 2023 by the authors. LA - English DB - MTMT ER - TY - JOUR AU - Chacón, L. AU - Kuropka, B. AU - González-Tortuero, E. AU - Schreiber, F. AU - Rojas-Jiménez, K. AU - Rodríguez-Rojas, A. TI - Mechanisms of low susceptibility to the disinfectant benzalkonium chloride in a multidrug-resistant environmental isolate of Aeromonas hydrophila JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 14 PY - 2023 SN - 1664-302X DO - 10.3389/fmicb.2023.1180128 UR - https://m2.mtmt.hu/api/publication/34126802 ID - 34126802 N1 - Evolutionary Biology, Institut für Biologie, Freie Universität Berlin, Berlin, Germany Health Research Institute, University of Costa Rica, San José, Costa Rica Division of Biodeterioration and Reference Organisms (4.1), Department of Materials and the Environment, Federal Institute for Materials Research and Testing (BAM), Berlin, Germany Institute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany School of Science, Engineering, and Environment (SEE), University of Salford, Manchester, United Kingdom Escuela de Biología, University of Costa Rica, San José, Costa Rica Small Animal Internal Medicine, Clinic for Small Animals, University of Veterinary Medicine (Vetmeduni), Vienna, Austria Export Date: 5 September 2023 Correspondence Address: Rodríguez-Rojas, A.; Evolutionary Biology, Germany; email: a.rojas@fu-berlin.de Correspondence Address: Rojas-Jiménez, K.; Escuela de Biología, Costa Rica; email: keilor.rojas@ucr.ac.cr Chemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; benzalkonium chloride, 66331-30-4, 78244-97-0, 81181-32-0; ceftazidime, 72558-82-8, 73547-61-2, 78439-06-2; chloramphenicol, 134-90-7, 2787-09-9, 56-75-7; ciprofloxacin, 85721-33-1, 86393-32-0, 128074-72-6, 128074-76-0, 192934-52-4, 93107-08-5, 86483-48-9, 96186-80-0; colistin, 1066-17-7, 1264-72-8; daptomycin, 103060-53-3; doxycycline, 10592-13-9, 17086-28-1, 564-25-0, 94088-85-4; fosfomycin, 23155-02-4; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; rifampicin, 13292-46-1; streptomycin, 57-92-1; tetracycline, 23843-90-5, 60-54-8, 64-75-5, 8021-86-1; trimethoprim, 738-70-5; vancomycin, 1404-90-6, 1404-93-9 Tradenames: Acclaim PepMap100 C18; DNeasy, Qiagen, United States; NovaSeq, Illumina; Ultimate 3000, Thermo Manufacturers: Greiner, Germany; Illumina; Roth, Germany; Merieux, France; Qiagen, United States; Thermo Funding details: B7-255, SFB973 Funding text 1: Deutscher Akademischer Austauschdienst (DAAD) Short-Term Grants, 2021 and the University o Costa Rica for the financial support of the LC internship in Germany. Vicerrectoría de Investigación of University of Costa Rica for the financial funds for the project B7-255. AR-R was supported by SFB973 from Deutsche Forschungsgemeinschaft ( http://www.sfb973.de/ ), project C5 ( https://www.sfb973.de/members_staff/c5/index.html ). Funding text 2: We thank Jens Rolff from Freie Universität Berlin for his support. For mass spectrometry, we would like to acknowledge the assistance of the Core Facility BioSupraMol, supported by the Deutsche Forschungsgemeinschaft (DFG). Furthermore, we acknowledge support from the Open Access Publication Fund of Freie Universität Berlin. AB - Excessive discharge of quaternary ammonium disinfectants such as benzalkonium chloride (BAC) into aquatic systems can trigger several physiological responses in environmental microorganisms. In this study, we isolated a less-susceptible strain of Aeromonas hydrophila to BAC, designated as INISA09, from a wastewater treatment plant in Costa Rica. We characterized its phenotypic response upon exposure to three different concentrations of BAC and characterized mechanisms related to its resistance using genomic and proteomic approaches. The genome of the strain, mapped against 52 different sequenced A. hydrophila strains, consists of approximately 4.6 Mb with 4,273 genes. We found a massive genome rearrangement and thousands of missense mutations compared to the reference strain A. hydrophila ATCC 7966. We identified 15,762 missense mutations mainly associated with transport, antimicrobial resistance, and outer membrane proteins. In addition, a quantitative proteomic analysis revealed a significant upregulation of several efflux pumps and the downregulation of porins when the strain was exposed to three BAC concentrations. Other genes related to membrane fatty acid metabolism and redox metabolic reactions also showed an altered expression. Our findings indicate that the response of A. hydrophila INISA09 to BAC primarily occurs at the envelop level, which is the primary target of BAC. Our study elucidates the mechanisms of antimicrobial susceptibility in aquatic environments against a widely used disinfectant and will help better understand how bacteria can adapt to biocide pollution. To our knowledge, this is the first study addressing the resistance to BAC in an environmental A. hydrophila isolate. We propose that this bacterial species could also serve as a new model to study antimicrobial pollution in aquatic environments. Copyright © 2023 Chacón, Kuropka, González-Tortuero, Schreiber, Rojas-Jiménez and Rodríguez-Rojas. LA - English DB - MTMT ER - TY - JOUR AU - Chaudhary, S. AU - Ali, Z. AU - Tehseen, M. AU - Haney, E.F. AU - Pantoja-Angles, A. AU - Alshehri, S. AU - Wang, T. AU - Clancy, G.J. AU - Ayach, M. AU - Hauser, C. AU - Hong, P.-Y. AU - Hamdan, S.M. AU - Hancock, R.E.W. AU - Mahfouz, M. TI - Efficient in planta production of amidated antimicrobial peptides that are active against drug-resistant ESKAPE pathogens JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 14 PY - 2023 IS - 1 SN - 2041-1723 DO - 10.1038/s41467-023-37003-z UR - https://m2.mtmt.hu/api/publication/33759558 ID - 33759558 N1 - Export Date: 19 April 2023 AB - Antimicrobial peptides (AMPs) are promising next-generation antibiotics that can be used to combat drug-resistant pathogens. However, the high cost involved in AMP synthesis and their short plasma half-life render their clinical translation a challenge. To address these shortcomings, we report efficient production of bioactive amidated AMPs by transient expression of glycine-extended AMPs in Nicotiana benthamiana line expressing the mammalian enzyme peptidylglycine α-amidating mono-oxygenase (PAM). Cationic AMPs accumulate to substantial levels in PAM transgenic plants compare to nontransgenic N. benthamiana. Moreover, AMPs purified from plants exhibit robust killing activity against six highly virulent and antibiotic resistant ESKAPE pathogens, prevent their biofilm formation, analogous to their synthetic counterparts and synergize with antibiotics. We also perform a base case techno-economic analysis of our platform, demonstrating the potential economic advantages and scalability for industrial use. Taken together, our experimental data and techno-economic analysis demonstrate the potential use of plant chassis for large-scale production of clinical-grade AMPs. © 2023, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Chen, H. AU - Wang, L. AU - Hu, M. TI - In vitro efficacy and combined drug sensitivity of lycosin-I against methicillin-resistant Staphylococcus aureus JF - CHINESE JOURNAL OF LABORATORY MEDICINE J2 - CHIN J LAB MED VL - 46 PY - 2023 IS - 6 SP - 604 EP - 611 PG - 8 SN - 1009-9158 DO - 10.3760/cma.j.cn114452-20230418-00443 UR - https://m2.mtmt.hu/api/publication/34126795 ID - 34126795 N1 - Export Date: 5 September 2023 Correspondence Address: Wang, L.; Department of Laboratory Medicine, China; email: jykwangling@csu.edu.cn Funding details: Natural Science Foundation of Fujian Province Funding details: Natural Science Foundation of Hunan Province, 2019JJ50892 Funding text 1: unan Natural Science Foundation (2019JJ50892) Funding text 2: ratio of MBC/MIC was 1‑2. The bactericidal kinetics test revealed that the number of surviving MRSA clinical isolates and standard strains initially decreased rapidly but then showed a resurgence when the concentration of lycosin‑Ⅰ was 1/2 MIC or MIC. While, the bacterial load gradually reduced until complete elimination when the concentration was at 2 MIC or 4 MIC. The combination of lycosin‑Ⅰ and gentamicin exhibited mainly synergistic effects, while the combination with other antibiotics showed mainly additive effects. Moreover, the combination of lycosin‑Ⅰ and antibacterial drugs can significantly reduce the MIC50 and MIC90 of antibiotics. Conclusion lycosin‑Ⅰ has great antibacterial and bactericidal activity against MRSA in vitro with rapid and thorough sterilization effect and it can play a synergistic or additive role when combined with other antibacterial drugs against MRSA in vitro. 【Key words】 Antimicrobial cationic peptides; Methicillin‑resistant Staphylococcus aureus Fund program: Hunan Natural Science Foundation (2019JJ50892) AB - Objective The aim of this study was to evaluate the in vitro activity of lysosin-Ⅰ against Methicillin-resistant Staphylococcus aureus (MRSA) and its synergistic effect with eight common antibacterial drugs against MRSA. Methods This study was conducted following the design principles of a randomized controlled trials. Ten MRSA isolates, clinically isolated from the Second Xiangya Hospital of Central South University between September and November 2021, were determined the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and bactericidal kinetic test of lycosin-Ⅰ in vitro anti-MRSA by micro-broth dilution method. Additionally, the micro-broth chessboard dilution method was utilized to evaluate the in vitro efficacy of lycosin-Ⅰ in combination with eight common antimicrobial agants, including penicillin, erythromycin, levofloxacin, gentamicin, rifampicin, minocycline, vancomycin, and linezolid. Results The MIC range of lycosin-Ⅰ against MRSA was found to be between 4-8 mg/L with the MIC50 and MIC90 were 4 mg/L and 8 mg/L, respectively. The range of MBC was also between 4-8 mg/L, and the ratio of MBC/MIC was 1-2. The bactericidal kinetics test revealed that the number of surviving MRSA clinical isolates and standard strains initially decreased rapidly but then showed a resurgence when the concentration of lycosin-Ⅰ was 1/2 MIC or MIC. While, the bacterial load gradually reduced until complete elimination when the concentration was at 2 MIC or 4 MIC. The combination of lycosin-Ⅰ and gentamicin exhibited mainly synergistic effects, while the combination with other antibiotics showed mainly additive effects. Moreover, the combination of lycosin-Ⅰ and antibacterial drugs can significantly reduce the MIC50 and MIC90 of antibiotics. Conclusion lycosin-Ⅰ has great antibacterial and bactericidal activity against MRSA in vitro with rapid and thorough sterilization effect and it can play a synergistic or additive role when combined with other antibacterial drugs against MRSA in vitro. © 2023 Authors. All rights reserved. LA - Chinese DB - MTMT ER - TY - JOUR AU - Cho, Timothy H. S. AU - Pick, Kat AU - Raivio, Tracy L. TI - Bacterial envelope stress responses: Essential adaptors and attractive targets JF - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH J2 - BBA-MOL CELL RES VL - 1870 PY - 2023 IS - 2 PG - 25 SN - 0167-4889 DO - 10.1016/j.bbamcr.2022.119387 UR - https://m2.mtmt.hu/api/publication/34770442 ID - 34770442 N1 - Funding Agency and Grant Number: National Sciences and Engineering Research Council; Canadian Institutes of Health Research [NSERC RGPIN-2021-02710]; AMR-One Health Consortium; Major Innovation Fund program of the Ministry of Jobs, Economy and Innovation, Government of Alberta; [CIHR MOP 142347] Funding text: This research was supported by operating grants from The National Sciences and Engineering Research Council (NSERC RGPIN-2021-02710) and The Canadian Institutes of Health Research (CIHR MOP 142347) , and a project grant from the AMR-One Health Consortium, funded by the Major Innovation Fund program of the Ministry of Jobs, Economy and Innovation, Government of Alberta, to T.R. AB - Millions of deaths a year across the globe are linked to antimicrobial resistant infections. The need to develop new treatments and repurpose of existing antibiotics grows more pressing as the growing antimicrobial resistance pandemic advances. In this review article, we propose that envelope stress responses, the signaling pathways bacteria use to recognize and adapt to damage to the most vulnerable outer compartments of the microbial cell, are attractive targets. Envelope stress responses (ESRs) support colonization and infection by responding to a plethora of toxic envelope stresses encountered throughout the body; they have been co-opted into virulence networks where they work like global positioning systems to coordinate adhesion, invasion, microbial warfare, and biofilm formation. We highlight progress in the development of therapeutic strategies that target ESR signaling proteins and adaptive networks and posit that further characterization of the molecular mechanisms governing these essential niche adaptation machineries will be important for sparking new therapeutic ap-proaches aimed at short-circuiting bacterial adaptation. LA - English DB - MTMT ER - TY - THES AU - Chung, Carolina H. TI - Leveraging Systems-level Metabolic Modeling and Machine Learning to Optimize Antibiotic Combination Therapy Design PY - 2023 SP - 180 DO - 10.7302/8201 UR - https://m2.mtmt.hu/api/publication/34524592 ID - 34524592 LA - English DB - MTMT ER - TY - JOUR AU - Coluzzi, Charles AU - Guillemet, Martin AU - Mazzamurro, Fanny AU - Touchon, Marie AU - Godfroid, Maxime AU - Achaz, Guillaume AU - Glaser, Philippe AU - Rocha, Eduardo P. C. TI - Chance Favors the Prepared Genomes: Horizontal Transfer Shapes the Emergence of Antibiotic Resistance Mutations in Core Genes JF - MOLECULAR BIOLOGY AND EVOLUTION J2 - MOL BIOL EVOL VL - 40 PY - 2023 IS - 10 PG - 20 SN - 0737-4038 DO - 10.1093/molbev/msad217 UR - https://m2.mtmt.hu/api/publication/34235446 ID - 34235446 N1 - Funding Agency and Grant Number: We acknowledge Eugen Pfeifer for providing the viral regions data and Manuel Ares-Arroyo for scientific discussions. This project was funded by the INCEPTION project Path2Resistance (PIA/ANR-16-CONV-0005), Equipe FRM (Equipe FRM/EQU201903007835), Laboratoi [PIA/ANR-16-CONV-0005]; INCEPTION project Path2Resistance [PIA/ANR-16-CONV-0005]; Equipe FRM [FRM/EQU201903007835]; Laboratoire d'Excellence IBEID [ANR-10-LABX-62-IBEID]; SEQ2DIAG [ANR-20-PAMR-0010]; Agence Nationale de la Recherche (ANR) [ANR-20-PAMR-0010] Funding Source: Agence Nationale de la Recherche (ANR) Funding text: We acknowledge Eugen Pfeifer for providing the viral regions data and Manuel Ares-Arroyo for scientific discussions. This project was funded by the INCEPTION project Path2Resistance (PIA/ANR-16-CONV-0005), Equipe FRM (Equipe FRM/EQU201903007835), Laboratoire d'Excellence IBEID (ANR-10-LABX-62-IBEID), and SEQ2DIAG (ANR-20-PAMR-0010). This work used the computational and storage services (MAESTRO cluster) provided by the IT department at Institut Pasteur, Paris. We thank Frederic Barras, Jessica El Khoury, and Laurence Van Melderen for help on the interpretation of our results. AB - Bacterial lineages acquire novel traits at diverse rates in part because the genetic background impacts the successful acquisition of novel genes by horizontal transfer. Yet, how horizontal transfer affects the subsequent evolution of core genes remains poorly understood. Here, we studied the evolution of resistance to quinolones in Escherichia coli accounting for population structure. We found 60 groups of genes whose gain or loss induced an increase in the probability of subsequently becoming resistant to quinolones by point mutations in the gyrase and topoisomerase genes. These groups include functions known to be associated with direct mitigation of the effect of quinolones, with metal uptake, cell growth inhibition, biofilm formation, and sugar metabolism. Many of them are encoded in phages or plasmids. Although some of the chronologies may reflect epidemiological trends, many of these groups encoded functions providing latent phenotypes of antibiotic low-level resistance, tolerance, or persistence under quinolone treatment. The mutations providing resistance were frequent and accumulated very quickly. Their emergence was found to increase the rate of acquisition of other antibiotic resistances setting the path for multidrug resistance. Hence, our findings show that horizontal gene transfer shapes the subsequent emergence of adaptive mutations in core genes. In turn, these mutations further affect the subsequent evolution of resistance by horizontal gene transfer. Given the substantial gene flow within bacterial genomes, interactions between horizontal transfer and point mutations in core genes may be a key to the success of adaptation processes. LA - English DB - MTMT ER - TY - JOUR AU - Costa, Beatriz de Aquino Marques da AU - Porto, Ana Lúcia Figueiredo AU - Oliveira, Vagne de Melo AU - Porto, Tatiana Souza TI - Bioactive collagen peptides: bibliometric approach and market trends for aquatic sources JF - Food Science Today J2 - fstoday VL - 2 PY - 2023 IS - 1 PG - 14 SN - 2965-1190 DO - 10.58951/fstoday.2023.17 UR - https://m2.mtmt.hu/api/publication/34075925 ID - 34075925 AB - The development of the collagen peptides industry is associated with new consumption trends among the population, projected to reach a value of 795 million USD by 2025. Given the relevance of the topic, the present work provides an overview of collagen, its sources, applications, as well as the properties and bioactivities of the peptides formed from its hydrolysis. In addition, a bibliometric analysis was performed to highlight a global overview of publication trends, co-authorship, and co-occurrence of keywords. Bibliometric data were obtained from the Web of Science platform using the descriptors “marine collagen peptide,” “marine collagen peptides,” “aquatic collagen peptide,” “aquatic collagen peptides,” “marine collagen hydrolysate,” “marine collagen hydrolysates,” “aquatic collagen hydrolysate” and “aquatic collagen hydrolysates” and the Boolean operator “OR,” to include terms that cover the possible variations used in the search. From the results obtained, articles and reviews published between 2000 and 2021 were selected. From the analysis of the data collected, it became evident the still relatively recent nature of the research on collagen peptides derived from aquatic organisms and the role of countries in Asia and Europe as leaders in the segment. The co-authorship analysis reveals that the collaboration network between authors/institutions is still scarce and strongly restricted to researchers/institutions in the same region. The co-occurrence analysis reveals that most of the works are related to the health area, highlighting its potential in the prevention/treatment of diseases. In view of the results obtained, it is expected that the growth of the production and commercial branch of these peptides will be accompanied by the amplification of collaboration between authors and institutions from different territories, establishing a global network of partnerships. LA - English DB - MTMT ER - TY - THES AU - de Oliveira, WS TI - Análise do perfil de virulência de Acinetobacter baumannii resistente à polimixina B PY - 2023 SP - 89 UR - https://m2.mtmt.hu/api/publication/35061589 ID - 35061589 LA - Portuguese DB - MTMT ER - TY - JOUR AU - Ding, Y. AU - Hao, J. AU - Xiao, W. AU - Ye, C. AU - Xiao, X. AU - Jian, C. AU - Tang, M. AU - Li, G. AU - Liu, J. AU - Zeng, Z. TI - Role of efflux pumps, their inhibitors, and regulators in colistin resistance JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 14 PY - 2023 SN - 1664-302X DO - 10.3389/fmicb.2023.1207441 UR - https://m2.mtmt.hu/api/publication/34126798 ID - 34126798 N1 - Export Date: 5 September 2023 Correspondence Address: Liu, J.; Department of Laboratory Medicine, China; email: liujb7203@swmu.edu.cn Correspondence Address: Zeng, Z.; Department of Laboratory Medicine, China; email: zengzhangrui@swmu.edu.cn Chemicals/CAS: azithromycin, 83905-01-5, 117772-70-0, 121470-24-4; carbonyl cyanide chlorophenylhydrazone, 32389-86-9, 555-60-2; cefepime, 88040-23-7; cephalosporin, 11111-12-9; chloramphenicol, 134-90-7, 2787-09-9, 56-75-7; chlorhexidine, 3697-42-5, 55-56-1; imipenem, 64221-86-9; nalidixic acid, 389-08-2; nitrofurantoin, 54-87-5, 67-20-9; nitroxoline, 4008-48-4; novobiocin, 1476-53-5, 303-81-1, 39301-00-3, 4309-70-0; octenidine, 71251-02-0; phosphoethanolamine, 1071-23-4, 29868-05-1; polymyxin B, 1404-26-8, 1405-20-5; reserpine, 50-55-5, 8001-95-4; rifampicin, 13292-46-1; salicylic acid, 63-36-5, 69-72-7; teicoplanin, 61036-62-2, 61036-64-4; tetracycline, 23843-90-5, 60-54-8, 64-75-5, 8021-86-1; tigecycline, 220620-09-7; trimethoprim, 738-70-5 Funding details: 2019ZQN017 Funding details: Sichuan Province Science and Technology Support Program, 2020YFQ0045, 2021YFS0329 Funding text 1: This work was supported by the Sichuan Science and Technology Program (2020YFQ0045 and 2021YFS0329) and School-level scientific research project of Southwest Medical University (2019ZQN017). AB - Colistin is highly promising against multidrug-resistant and extensively drug-resistant bacteria clinically. Bacteria are resistant to colistin mainly through mcr and chromosome-mediated lipopolysaccharide (LPS) synthesis-related locus variation. However, the current understanding cannot fully explain the resistance mechanism in mcr-negative colistin-resistant strains. Significantly, the contribution of efflux pumps to colistin resistance remains to be clarified. This review aims to discuss the contribution of efflux pumps and their related transcriptional regulators to colistin resistance in various bacteria and the reversal effect of efflux pump inhibitors on colistin resistance. Previous studies suggested a complex regulatory relationship between the efflux pumps and their transcriptional regulators and LPS synthesis, transport, and modification. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), 1-(1-naphthylmethyl)-piperazine (NMP), and Phe-Arg-β-naphthylamide (PAβN) all achieved the reversal of colistin resistance, highlighting the role of efflux pumps in colistin resistance and their potential for adjuvant development. The contribution of the efflux pumps to colistin resistance might also be related to specific genetic backgrounds. They can participate in colistin tolerance and heterogeneous resistance to affect the treatment efficacy of colistin. These findings help understand the development of resistance in mcr-negative colistin-resistant strains. Copyright © 2023 Ding, Hao, Xiao, Ye, Xiao, Jian, Tang, Li, Liu and Zeng. LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Hess, Claudia AU - Ganas, Petra AU - Boros, Zsófia AU - Kiss, János AU - Makrai, László AU - Dublecz, Károly AU - Pál, László AU - Fodor, László AU - Sebestyén, Anna AU - Klein, Michael G. AU - Tarasco, Eustachio AU - Kulkarni, Manjusha M. AU - McGwire, Bradford S. AU - Vellai, Tibor AU - Hess, Michael TI - Antimicrobial Peptides (AMP) in the Cell-Free Culture Media of Xenorhabdus budapestensis and X. szentirmaii Exert Anti-Protist Activity against Eukaryotic Vertebrate Pathogens including Histomonas meleagridis and Leishmania donovani Species JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 12 PY - 2023 IS - 9 PG - 25 SN - 2079-6382 DO - 10.3390/antibiotics12091462 UR - https://m2.mtmt.hu/api/publication/34147172 ID - 34147172 N1 - Department of Genetics, Institute of Biology, Eötvös Loránd University, Pázmány Péter. sétány 1C, Budapest, H-1117, Hungary Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine (Vetmeduni Vienna), Vienna, 1210, Austria Agribiotechnology and Precision Breeding for Food Security National Laboratory, Department of Microbiology and Applied Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Páter Károly utca 1, Gödöllő, H-2100, Hungary Autovakcina Kft, Budapest, H-1171, Hungary Institute of Physiology and Nutrition, Georgikon Campus, Hungarian University of Agriculture and Life Sciences (MATE), Deák Ferenc utca 16, Keszthely, H-8360, Hungary Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, H-1143, Hungary First Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, H-1085, Hungary USDA-ARS, Department of Entomology, The Ohio State University, 13416 Claremont Ave, Cleveland, OH 44130, United States Department of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, Via Amendola 165/A, Bari, 70126, Italy Division of Infectious Diseases, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, United States Export Date: 12 October 2023 Correspondence Address: Fodor, A.; Department of Genetics, Pázmány Péter. sétány 1C, Hungary; email: fodorandras@yahoo.com Correspondence Address: Hess, M.; Clinic for Poultry and Fish Medicine, Austria; email: michael.hess@vetmeduni.ac.at AB - Anti-microbial peptides provide a powerful toolkit for combating multidrug resistance. Combating eukaryotic pathogens is complicated because the intracellular drug targets in the eukaryotic pathogen are frequently homologs of cellular structures of vital importance in the host organism. The entomopathogenic bacteria (EPB), symbionts of entomopathogenic–nematode species, release a series of non-ribosomal templated anti-microbial peptides. Some may be potential drug candidates. The ability of an entomopathogenic–nematode/entomopathogenic bacterium symbiotic complex to survive in a given polyxenic milieu is a coevolutionary product. This explains that those gene complexes that are responsible for the biosynthesis of different non-ribosomal templated anti-microbial protective peptides (including those that are potently capable of inactivating the protist mammalian pathogen Leishmania donovanii and the gallinaceous bird pathogen Histomonas meleagridis) are co-regulated. Our approach is based on comparative anti-microbial bioassays of the culture media of the wild-type and regulatory mutant strains. We concluded that Xenorhabdus budapestensis and X. szentirmaii are excellent sources of non-ribosomal templated anti-microbial peptides that are efficient antagonists of the mentioned pathogens. Data on selective cytotoxicity of different cell-free culture media encourage us to forecast that the recently discovered “easy-PACId” research strategy is suitable for constructing entomopathogenic-bacterium (EPB) strains producing and releasing single, harmless, non-ribosomal templated anti-microbial peptides with considerable drug, (probiotic)-candidate potential. LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Vellai, Tibor AU - Hess, Claudia AU - Makrai, László AU - Dublecz, Károly AU - Pál, László AU - Molnár, Andor AU - Klein, Michael G. AU - Tarasco, Eustachio AU - Józsa, Sándor AU - Ganas, Petra AU - Hess, Michael TI - XENOFOOD—An Autoclaved Feed Supplement Containing Autoclavable Antimicrobial Peptides—Exerts Anticoccidial GI Activity, and Causes Bursa Enlargement, but Has No Detectable Harmful Effects in Broiler Cockerels despite In Vitro Detectable Cytotoxicity on LHM Cells JF - PATHOGENS J2 - PATHOGENS VL - 12 PY - 2023 IS - 3 PG - 20 SN - 2076-0817 DO - 10.3390/pathogens12030458 UR - https://m2.mtmt.hu/api/publication/33704277 ID - 33704277 N1 - Export Date: 19 April 2023 AB - Entomopathogenic bacteria are obligate symbionts of entomopathogenic nematode (EPN) species. These bacteria biosynthesize and release non-ribosomal-templated hybrid peptides (NR-AMPs), with strong, and large-spectral antimicrobial potential, capable of inactivating pathogens belonging to different prokaryote, and eukaryote taxa. The cell-free conditioned culture media (CFCM) of Xenorhabdus budapestensis and X. szentirmaii efficiently inactivate poultry pathogens like Clostridium, Histomonas, and Eimeria. To learn whether a bio-preparation containing antimicrobial peptides of Xenorhabdus origin with accompanying (in vitro detectable) cytotoxic effects could be considered a safely applicable preventive feed supplement, we conducted a 42-day feeding experiment on freshly hatched broiler cockerels. XENOFOOD (containing autoclaved X. budapestensis, and X. szentirmaii cultures developed on chicken food) were consumed by the birds. The XENOFOOD exerted detectable gastrointestinal (GI) activity (reducing the numbers of the colony-forming Clostridium perfringens units in the lower jejunum. No animal was lost in the experiment. Neither the body weight, growth rate, feed-conversion ratio, nor organ-weight data differed between the control (C) and treated (T) groups, indicating that the XENOFOOD diet did not result in any detectable adverse effects. We suppose that the parameters indicating a moderate enlargement of bursas of Fabricius (average weight, size, and individual bursa/spleen weight-ratios) in the XENOFOOD-fed group must be an indirect indication that the bursa-controlled humoral immune system neutralized the cytotoxic ingredients of the XENOFOOD in the blood, not allowing to reach their critical cytotoxic concentration in the sensitive tissues. LA - English DB - MTMT ER - TY - CHAP AU - Francis, F. AU - Chaudhary, N. ED - Ajesh, K ED - Sreejith, K TI - Antimicrobial peptides: features and modes of action T2 - Antimicrobial Peptides PB - Elsevier CY - London SN - 9780323856829 PY - 2023 SP - 33 EP - 65 PG - 33 DO - 10.1016/B978-0-323-85682-9.00016-7 UR - https://m2.mtmt.hu/api/publication/34025065 ID - 34025065 N1 - Export Date: 20 June 2023 AB - Antimicrobial peptides (AMPs) or host defense peptides are short amphipathic, generally cationic, evolutionarily conserved biomolecules that constitute the first line of defense in most living organisms. These peptides display activity against Gram-negative and Gram-positive bacteria, fungi, and some protozoa and viruses. AMPs can be linear or cyclic; the cyclic structure is achieved through end-to-end ligation or through disulfide bridge(s) between the Cys residues near the termini. Structurally, AMPs can be classified as α-helical, β-sheets, αβ, and non-αβ. Another useful classification could be disulfide-containing and disulfide-lacking peptides. Most AMPs fold into conformations that introduce amphipathicity, a feature that enables them to interact with membranes. Understandably, a large majority of AMPs exhibit antimicrobial activity through membrane-destabilization, which causes leakage of cellular content, thereby causing cell death. Although the exact mechanism of action is an enigma and could be peptide-specific, several models have been proposed for their antimicrobial action. These include the barrel-stave model, the carpet model, the toroidal pore model, and the detergent model. Apart from the antimicrobial activity, AMPs could possess anticancer activity, antimycoplasmal activity, spermicidal activity, cause activation of the immune response, and regulation of inflammation. These attributes of AMPs make them promising candidates as an alternative to conventional antibiotics. © 2023 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Gagat, P. AU - Duda-Madej, A. AU - Ostrówka, M. AU - Pietluch, F. AU - Seniuk, A. AU - Mackiewicz, P. AU - Burdukiewicz, M. TI - Testing Antimicrobial Properties of Selected Short Amyloids JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 1 SN - 1661-6596 DO - 10.3390/ijms24010804 UR - https://m2.mtmt.hu/api/publication/33597268 ID - 33597268 N1 - Export Date: 27 January 2023 AB - Amyloids and antimicrobial peptides (AMPs) have many similarities, e.g., both kill microorganisms by destroying their membranes, form aggregates, and modulate the innate immune system. Given these similarities and the fact that the antimicrobial properties of short amyloids have not yet been investigated, we chose a group of potentially antimicrobial short amyloids to verify their impact on bacterial and eukaryotic cells. We used AmpGram, a best-performing AMP classification model, and selected ten amyloids with the highest AMP probability for our experimental research. Our results indicate that four tested amyloids: VQIVCK, VCIVYK, KCWCFT, and GGYLLG, formed aggregates under the conditions routinely used to evaluate peptide antimicrobial properties, but none of the tested amyloids exhibited antimicrobial or cytotoxic properties. Accordingly, they should be included in the negative datasets to train the next-generation AMP prediction models, based on experimentally confirmed AMP and non-AMP sequences. In the article, we also emphasize the importance of reporting non-AMPs, given that only a handful of such sequences have been officially confirmed. © 2023 by the authors. LA - English DB - MTMT ER - TY - JOUR AU - Gong, Haoning AU - Hu, Xuzhi AU - Zhang, Lin AU - Fa, Ke AU - Liao, Mingrui AU - Liu, Huayang AU - Fragneto, Giovanna AU - Campana, Mario AU - Lu, Jian Ren TI - How do antimicrobial peptides disrupt the lipopolysaccharide membrane leaflet of Gram-negative bacteria? JF - JOURNAL OF COLLOID AND INTERFACE SCIENCE J2 - J COLLOID INTERF SCI VL - 637 PY - 2023 SP - 182 EP - 192 PG - 11 SN - 0021-9797 DO - 10.1016/j.jcis.2023.01.051 UR - https://m2.mtmt.hu/api/publication/33617652 ID - 33617652 N1 - CAplus AN 2023:176781; MEDLINE PMID: 36701864 (Journal; Article); AB - It is widely regarded that antimicrobial peptides (AMPs) kill bacteria by phys. disrupting microbial membranes and causing cytoplasmic leakage, but it remains unclear how AMPs disrupt the outer membrane (OM) of Gram-neg. bacteria (GNB) and then compromise the inner membrane. We hypothesise that different AMPs impose different structural disruptions, with direct implications to their antimicrobial efficacies. The antimicrobial activities of three typical AMPs, including the designed short AMP, G3, and two natural AMPs, melittin and LL37, against E. coli and their haemolytic activities were studied. Lipopolysaccharide (LPS) and anionic di-palmitoyl phosphatidyl glycerol (DPPG) monolayer models were constructed to mimic the outer membrane and inner membrane leaflets of Gram-neg. bacteria. The binding and penetration of AMPs to the model lipid monolayers were systematically studied by neutron reflection via multiple H/D contrast variations. G3 has relatively high antimicrobial activity, low cytotoxicity, and high proteolytic stability, while melittin has significant haemolysis and LL37 has weaker antimicrobial activity. G3 could rapidly lyse LPS and DPPG monolayers within 10-20 min. In contrast, melittin was highly active against the LPS membrane, but the dynamic process lasted up to 80 min, with excessive stacking in the OM. LL37 caused rather weak destruction to LPS and DPPG monolayers, leading to massive adsorption on the membrane surface without penetrating the lipid tail region. These findings demonstrate that the rationally designed AMP G3 was well optimized to impose most effective destruction to bacterial membranes, consistent with its highest bactericidal activity. These different interfacial structural features associated with AMP binding shed light on the future development of active and biocompatible AMPs for infection and wound treatments. LA - English DB - MTMT ER - TY - JOUR AU - Grézal, Gábor AU - Spohn, Réka AU - Méhi, Orsolya Katinka AU - Dunai, Anett AU - Lázár, Viktória AU - Bálint, Balázs AU - Nagy, István AU - Pál, Csaba AU - Papp, Balázs TI - Plasticity and stereotypic rewiring of the transcriptome upon bacterial evolution of antibiotic resistance JF - MOLECULAR BIOLOGY AND EVOLUTION J2 - MOL BIOL EVOL VL - 40 PY - 2023 IS - 2 PG - 16 SN - 0737-4038 DO - 10.1093/molbev/msad020 UR - https://m2.mtmt.hu/api/publication/33632100 ID - 33632100 N1 - Funding Agency and Grant Number: Lendulet program of the Hungarian Academy of Sciences [LP-2009-013/2012, LP-2012-32/2018]; ELKH Lenduelet program [LP-2017-2010/2020]; Wellcome Trust WT [098016/Z/11/Z]; European Research Council [648364, 862077]; National Research, Development and Innovation Office; Ministry for Innovation and Technology [KKP KH125616, 126506, RRF-2.3.1-21-2022-00006, GINOP-2.3.2-15-2016-00026, GINOP-2.3.2-15-2 016-00014, GINOP-2.3.2-15-2 016-00020]; National Laboratory of Biotechnology Grant [2022-2.1.1-NL-2022-00008]; European Union [739593]; NKFIH [FK124254]; Janos Bolyai Research Fellowship from the Hungarian Academy of Sciences [BO/608/21] Funding text: This work was supported by the "Lendulet" program of the Hungarian Academy of Sciences LP-2009-013/2012 (B.P.), LP-2012-32/2018 (C.P.), the ELKH Lenduelet program LP-2017-2010/2020 (C.P.), the Wellcome Trust WT 098016/Z/11/Z (B.P.), The European Research Council H2020-ERC-2014-CoG 648364-Resistance Evolution (C.P.), and H2020-ERC-2019-PoC 862077-Aware (C.P.), the National Research, Development and Innovation Office and the Ministry for Innovation and Technology under the "Frontline" program KKP KH125616 and 126506 (B.P. and C.P.), RRF-2.3.1-21-2022-00006 (B.P.), GINOP-2.3.2-15-2016-00026 (iChamber, B.P.), GINOP-2.3.2-15-2 016-00014 (EVOMER, C.P. and B.P.), GINOP-2.3.2-15-2 016-00020 (MolMedEx TUMORDNS, C.P.), National Laboratory of Biotechnology Grant 2022-2.1.1-NL-2022-00008 (C.P. and B.P.), The European Union's Horizon 202 0 research and innovation program under grant agreement No 739593 (B.P.). NKFIH grant FK124254 (O.M.), the Janos Bolyai Research Fellowship from the Hungarian Academy of Sciences BO/608/21 (R.S.). AB - Bacterial evolution of antibiotic resistance frequently has deleterious side effects on microbial growth, virulence, and susceptibility to other antimicrobial agents. However, it is unclear how these trade-offs could be utilized for manipulating antibiotic resistance in the clinic, not least because the underlying molecular mechanisms are poorly understood. Using laboratory evolution, we demonstrate that clinically relevant resistance mutations in Escherichia coli constitutively rewire a large fraction of the transcriptome in a repeatable and stereotypic manner. Strikingly, lineages adapted to functionally distinct antibiotics and having no resistance mutations in common show a wide range of parallel gene expression changes that alter oxidative stress response, iron homeostasis, and the composition of the bacterial outer membrane and cell surface. These common physiological alterations are associated with changes in cell morphology and enhanced sensitivity to antimicrobial peptides. Finally, the constitutive transcriptomic changes induced by resistance mutations are largely distinct from those induced by antibiotic stresses in the wild-type. This indicates a limited role for genetic assimilation of the induced antibiotic stress response during resistance evolution. Our work suggests that diverse resistance mutations converge on similar global transcriptomic states that shape genetic susceptibility to antimicrobial compounds. LA - English DB - MTMT ER - TY - PAT AU - Hammond, K AU - Upton, M AU - Ryadnov, M TI - Antimicrobial peptides CY - Country:10017(13) PY - 2023 UR - https://m2.mtmt.hu/api/publication/34075919 ID - 34075919 LA - English DB - MTMT ER - TY - JOUR AU - He, Q. AU - Yang, Z. AU - Zou, Z. AU - Qian, M. AU - Wang, X. AU - Zhang, X. AU - Yin, Z. AU - Wang, J. AU - Ye, X. AU - Liu, D. AU - Guo, M. TI - Combating Escherichia coli O157:H7 with Functionalized Chickpea-Derived Antimicrobial Peptides JF - ADVANCED SCIENCE J2 - ADV SCI VL - 10 PY - 2023 IS - 6 SN - 2198-3844 DO - 10.1002/advs.202205301 UR - https://m2.mtmt.hu/api/publication/34888503 ID - 34888503 LA - English DB - MTMT ER - TY - JOUR AU - Hernando-Amado, S. AU - Laborda, P. AU - Martínez, J.L. TI - Tackling antibiotic resistance by inducing transient and robust collateral sensitivity JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 14 PY - 2023 IS - 1 SN - 2041-1723 DO - 10.1038/s41467-023-37357-4 UR - https://m2.mtmt.hu/api/publication/33759556 ID - 33759556 N1 - Export Date: 19 April 2023 AB - Collateral sensitivity (CS) is an evolutionary trade-off traditionally linked to the mutational acquisition of antibiotic resistance (AR). However, AR can be temporally induced, and the possibility that this causes transient, non-inherited CS, has not been addressed. Mutational acquisition of ciprofloxacin resistance leads to robust CS to tobramycin in pre-existing antibiotic-resistant mutants of Pseudomonas aeruginosa. Further, the strength of this phenotype is higher when nfxB mutants, over-producing the efflux pump MexCD-OprJ, are selected. Here, we induce transient nfxB-mediated ciprofloxacin resistance by using the antiseptic dequalinium chloride. Notably, non-inherited induction of AR renders transient tobramycin CS in the analyzed antibiotic-resistant mutants and clinical isolates, including tobramycin-resistant isolates. Further, by combining tobramycin with dequalinium chloride we drive these strains to extinction. Our results support that transient CS could allow the design of new evolutionary strategies to tackle antibiotic-resistant infections, avoiding the acquisition of AR mutations on which inherited CS depends. © 2023, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Huang, J. AU - Xu, Y. AU - Xue, Y. AU - Huang, Y. AU - Li, X. AU - Chen, X. AU - Xu, Y. AU - Zhang, D. AU - Zhang, P. AU - Zhao, J. AU - Ji, J. TI - Identification of potent antimicrobial peptides via a machine-learning pipeline that mines the entire space of peptide sequences JF - NATURE BIOMEDICAL ENGINEERING J2 - NAT BIOMED ENG VL - 7 PY - 2023 IS - 6 SP - 797 EP - 810 PG - 14 SN - 2157-846X DO - 10.1038/s41551-022-00991-2 UR - https://m2.mtmt.hu/api/publication/34887943 ID - 34887943 LA - English DB - MTMT ER - TY - THES AU - Huynh, Anh TI - Impacts of Collateral Effects and Spatial Heterogeneity on the Evolution of Resistance in Enterococcus PY - 2023 SP - 113 DO - 10.7302/8398 UR - https://m2.mtmt.hu/api/publication/34524601 ID - 34524601 LA - English DB - MTMT ER - TY - JOUR AU - Jangir, Pramod Kumar AU - Ogunlana, Lois AU - Szili, Petra AU - Czikkely, Márton Simon AU - Shaw, Liam P AU - Stevens, Emily J AU - Yang, Yu AU - Yang, Qiue AU - Wang, Yang AU - Pál, Csaba AU - Walsh, Timothy R AU - MacLean, Craig R TI - The evolution of colistin resistance increases bacterial resistance to host antimicrobial peptides and virulence JF - ELIFE J2 - ELIFE VL - 12 PY - 2023 PG - 22 SN - 2050-084X DO - 10.7554/eLife.84395 UR - https://m2.mtmt.hu/api/publication/33773756 ID - 33773756 N1 - Funding Agency and Grant Number: Wellcome Trust [106918/Z/15Z]; Medical Research Council [MR/S013768/1]; National Natural Science Foundation of China [81861138051]; European Research Council [GINOP-2.3.2-15-2016-00014, GINOP-2.3.2-15-2016-00020]; Hungarian Academy of Sciences Momentum [BB/M011224/1]; National Research, Development and Innovation Office [UNKP-21-4-New]; National Laboratories Program, National Laboratory of Biotechnology Grant [H2020-ERC-2019-PoC 862077]; National Research, Development and Innovation Office, Hungary; Biotechnology and Biological Sciences Research Council [LP -2017-10/2017]; Gazdasagfejlesztesi es Innovacios Operativ Program; Biotechnology and Biological Sciences Research Council; Ministry for Innovation and Technology Funding text: Wellcome Trust 106918/Z/15Z Craig R MacLeanMedical Research Council MR/S013768/1 Craig R MacLean Timothy R WalshNational Natural Science Foundation of China 81861138051 Yang WangEuropean Research Council H2020-ERC-2014-CoG 648364 -Resistance Evolution Csaba PalEuropean Research Council H2020-ERC-2019-PoC 862077-Aware Csaba PalHungarian Academy of Sciences Momentum 'Celzott Lenduelet' Programme LP -2017-10/2017 Csaba Pal National Research, Development and Innovation Office Elvonal' Programme KKP 126506 Csaba PalNational Laboratories Program, National Laboratory of Biotechnology Grant NKFIH-871-3/2020 Csaba Pal Gazdasagfejlesztesi es Innovacios Operativ Program GINOP-2.3.2-15-2016-00014 Csaba PalGazdasagfejlesztesi es Innovacios Operativ Program GINOP-2.3.2-15-2016-00020 (MolMedEx TUMORDNS Csaba PalBiotechnology and Biological Sciences Research Council BB/M011224/1 Liam P ShawMinistry for Innovation and Technology UNKP-21-4-New Petra Szili Ministry for Innovation and Technology FEIF/433-4/2020-ITM_ SZERZ Marton CzikkelyThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. For the purpose of Open Access, the authors have applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. AB - Antimicrobial peptides (AMPs) offer a promising solution to the antibiotic resistance crisis. However, an unresolved serious concern is that the evolution of resistance to therapeutic AMPs may generate cross-resistance to host AMPs, compromising a cornerstone of the innate immune response. We systematically tested this hypothesis using globally disseminated mobile colistin resistance (MCR) that has been selected by the use of colistin in agriculture and medicine. Here, we show that MCR provides a selective advantage to Escherichia coli in the presence of key AMPs from humans and agricultural animals by increasing AMP resistance. Moreover, MCR promotes bacterial growth in human serum and increases virulence in a Galleria mellonella infection model. Our study shows how the anthropogenic use of AMPs can drive the accidental evolution of resistance to the innate immune system of humans and animals. These findings have major implications for the design and use of therapeutic AMPs and suggest that MCR may be difficult to eradicate, even if colistin use is withdrawn. LA - English DB - MTMT ER - TY - JOUR AU - Khan, Z.A. AU - Warden, A.R. AU - Jiang, H. AU - Abdullah, A. AU - Ahmad, M. AU - Jiang, L. AU - Ding, X. TI - Time-lapse proteomics unveil constant high exposure of non-antibiotic drug induces synthetic susceptibility towards regular antibiotics JF - MICROBIOLOGICAL RESEARCH J2 - MICROBIOL RES VL - 269 PY - 2023 SN - 0944-5013 DO - 10.1016/j.micres.2023.127320 UR - https://m2.mtmt.hu/api/publication/33688217 ID - 33688217 N1 - Department of Anesthesiology and Surgical Intensive Care Unit, Xinhua Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, Shanghai Jiao Tong University, Shanghai, China State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, China Export Date: 8 March 2023 CODEN: MCRSE Correspondence Address: Jiang, L.; Department of Anesthesiology and Surgical Intensive Care Unit, China; email: jianglai@xinhuamed.com.cn Correspondence Address: Ding, X.; Department of Anesthesiology and Surgical Intensive Care Unit, China; email: dingxianting@sjtu.edu.cn AB - Antibiotic resistance is a significant threat to the human race, as regular consumption of antibiotics may lead to antibiotic-resistant bacterial strains. Non-antibiotic drugs also have an extensive impact on bacterial strains, where persistent uptake alters the survival mechanisms of bacteria that could lead to cross-resistance towards other antibiotics. Here, we use time-lapse proteomics shift assays to examine Gram-negative (E. coli. O157:H7 and P. aeruginosa) and Gram-positive (E. faecalis and S. aureus) strains of bacteria for short and continuous exposure to the non-antibiotic drug Hydroxychloroquine (HCQ). Proteomic transitions from wild type to HCQ-exposed strains revealed bacterial transitions and their survival adaptabilities, which were different across all strains. In addition to their structural differences, some shared pathways were enriched among Gram-negative and positive strains. We also validated the cross-resistance and sensitivity towards 24 regularly prescribed antibiotics, indicating that long-term exposure to non-antibiotic drugs may induce general proteomics alterations in the bacterial strains, promoting antibiotic resistance. We validated that HCQ exposure renders Gram-negative strains resistant to Β-lactam and susceptible to macrolides and folic acid. In contrast, Gram-positive strains become susceptible to Β-lactam and resistant to aminoglycosides. Exposure to non-antibiotic drugs causes resistance or susceptibility toward other antibiotics, providing clinicians a reason to overcome antibiotic resistance. © 2023 Elsevier GmbH LA - English DB - MTMT ER - TY - JOUR AU - Kherwal, M. AU - Gupta, A. AU - Jacob, M. AU - Chandra, A. AU - Sahu, P.K. AU - Goel, V.K. AU - Kumar, V. TI - Recent advances in self-assembled cyclic peptide-based smart nanos-tructures JF - Applied Chemical Engineering J2 - ACE VL - 6 PY - 2023 IS - 1 SP - 104 EP - 116 PG - 13 SN - 2578-2010 DO - 10.24294/ace.v6i1.1989 UR - https://m2.mtmt.hu/api/publication/34126799 ID - 34126799 N1 - School of Physical Sciences, Jawaharlal Nehru University, Delhi, 110067, India Department of Chemistry, Sri Venkateshwara College, University of Delhi, Delhi, 110021, India Department of Chemistry, Shivaji College, University of Delhi, Delhi, 110027, India Special Centre for Nano Science, Jawaharlal Nehru University, Delhi, 110067, India Export Date: 5 September 2023 Correspondence Address: Goel, V.K.; School of Physical Sciences, India; email: vijaykgoel@mail.jnu.ac.in Correspondence Address: Kumar, V.; Special Centre for Nano Science, India; email: kumarv@mail.jnu.ac.in Funding details: Jawaharlal Nehru University, JNU Funding details: Council of Scientific and Industrial Research, India, CSIR, 09/0263, 15268 Funding details: Saudi Pharmaceutical Society, SPS Funding details: Advanced Instrumentation Research Facility, AIRF Funding text 1: One of the authors Monika thanks Council of Scientific & Industrial Research (File no. 09/0263(15268)/2022-EMR-I) for Junior Research Fellowship. Author Vijay Kumar Goel would like to thank Sanganeria Foundation for financial support. The authors also show gratitude to AIRF, JNU (In-dia), SPS, and SCNS JNU (India) for instrumentation facilities. AB - Peptide chemistry has emerged as one of the growing fields of research. Peptide chemistry has positively impacted various areas, including biochemistry, medicine, hormonal therapy, drug delivery, food and the cosmetic industry, materials science, and nanotechnology, via the development of ways to change and imitate the shape and function of peptide structures. The structural changes of peptides and the employment of innovative synthetic techniques have left an indelible mark on a number of scientific disciplines. Numerous nanostructures based on simple and complicated peptides have been constructed so far; however, cyclic peptides have attracted a great deal of interest from the scientific community due to their wide range of applications and distinctive properties. These properties include self-assembly, morphogenesis, and charge distribution, among others. In addition, nanostructured cyclic peptides offer increased and effective performance due to their high stability, prolonged plasma half-life, membrane permeability, and efficient transport, among other attributes. Recent work indicates the manufacture of nanostructured cyclic peptides by chemical means. In this review, a brief investigation of the morphology of cyclic peptides was conducted. In addition, the therapeutic potential of these nanostructured cyclic peptides and the prognosis for a variety of potential applications are also discussed. © 2023 by author(s). LA - English DB - MTMT ER - TY - JOUR AU - Liao, Chien-Sen AU - Cao, Xuan-Di AU - Lee, Wei-Chen AU - Yang, Chu-Wen TI - The Effects of Preservatives on Antibiotic- and Preservative-Resistant Microbes and Nitrogen/Sulfur Cycle Associated Microbial Communities in Freshwater River Sediments JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 12 PY - 2023 IS - 7 SN - 2079-6382 DO - 10.3390/antibiotics12071082 UR - https://m2.mtmt.hu/api/publication/34136782 ID - 34136782 N1 - Department of Biological Science and Technology, I-Shou University, Kaohsiung, 82445, Taiwan Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, 84001, Taiwan Department of Microbiology, Soochow University, Taipei City, 111002, Taiwan Cited By :1 Export Date: 16 September 2024 Correspondence Address: Yang, C.-W.; Department of Microbiology, Taiwan; email: ycw6861@scu.edu.tw Chemicals/CAS: 4 hydroxybenzoic acid, 456-23-5, 99-96-7; benzoic acid, 532-32-1, 582-25-2, 65-85-0, 766-76-7; dehydroacetic acid, 16807-48-0, 4418-26-2, 45990-85-0, 520-45-6, 771-03-9; methane, 74-82-8; nitrate, 14797-55-8; nitrogen, 7727-37-9; sulfamethoxazole, 723-46-6; tetracycline, 23843-90-5, 60-54-8, 64-75-5, 8021-86-1 Tradenames: MiSeq, Illumina, United States; PowerSoil, Qiagen, Netherlands Manufacturers: Agilent; Mettler Toledo, Switzerland; Chromtech, China; Merck, Germany; Whatman, United Kingdom; Qiagen, Netherlands; Illumina, United States AB - The intensive use of benzoic acid (BA), 4-hydroxybenzoic acid (HB), and dehydroacetate (DHA) as additives and preservatives in cosmetics and foods causes emerging environmental pollutions. Anthropogenic releases of BA, HB and DHA are primarily emissions into water and soil. However, few studies investigate the effects of BA, HB and DHA on microbial communities in freshwater river sediments. The aim of this study is to reveal the effects of BA, HB and DHA on microbial communities in freshwater river sediments. Tetracycline-, sulfamethoxazole- and preservative-resistant microbes were increased in the river sediments treated with BA, HB and DHA. The relative abundances of methanogen- and xenobiotic-degradation-associated microbial communities were also increased in the BA-, HB- and DHA-treated sediments. The relative abundance of four nitrogen cycle associated microbial groups (anammox, nitrogen fixation, denitrification, and dissimilatory nitrate reduction) were increased after the eighth week in the BA-, HB- and DHA-treated sediments. For the sulfur cycle, the relative abundance of thiosulfate oxidation associated microbial communities were increased after the eighth week in the BA-, HB- and DHA-treated sediments. Results of this study provide insight into the effects of BA, HB and DHA on antibiotic resistance, nitrogen cycle, sulfur cycle, drug resistance and methane production in freshwater aquatic environments. LA - English DB - MTMT ER - TY - JOUR AU - Li, Li AU - Wang, Yinglu AU - Huang, Zhengjun AU - Xu, Zuxian AU - Cao, Ruipin AU - Li, Jiaxin AU - Wu, Biyi AU - Lu, Jian Ren AU - Zhu, Hu TI - An additive-free multifunctional β-glucan-peptide hydrogel participates in the whole process of bacterial-infected wound healing JF - JOURNAL OF CONTROLLED RELEASE J2 - J CONTROL RELEASE VL - 362 PY - 2023 SP - 577 EP - 590 PG - 14 SN - 0168-3659 DO - 10.1016/j.jconrel.2023.09.010 UR - https://m2.mtmt.hu/api/publication/34312619 ID - 34312619 N1 - Export Date: 28 November 2023; CODEN: JCREE AB - Bacterial infections and excessive inflammation can impede the healing of wounds. Hydrogels have emerged as a promising approach for dressing bacterial-infected injuries. However, some antibacterial hydrogels are complex, costly, and even require assistance with other instruments such as light, making them unsuitable for routine outdoor injuries. Here, we developed an in-situ generating hydrogel via hybridizing oxidized beta-D-glucan with antimicrobial peptide C(8)G(2) through the Schiff base reaction. This hydrogel is easily accessible and actively contributes to the whole healing process of bacterial-infected wounds, demonstrating remarkable antibacterial activity and biological compatibility. The pH-sensitive reversible imine bond enables the hydrogel to self-heal and sustainably release the antibacterial peptide, thereby improving its bioavailability and reducing toxicity. Meanwhile, the immunoregulating beta-D-glucan inhibits the release of inflammatory factors while promoting the release of anti-inflammatory factors. In methicillin-resistant Staphylococcus aureus (MRSA)-infected full-thickness skin wound models, the hybrid hydrogel showed superior antibacterial and anti-inflammatory activity, enhanced the M2 macrophage polarization, expedited wound closure, and regenerated epidermis tissue. These features make this hydrogel an appealing wound dressing for treating multi-drug-resistant bacteria-infected wounds. LA - English DB - MTMT ER - TY - JOUR AU - Liu, D.Y. AU - Phillips, L. AU - Wilson, D.M. AU - Fulton, K.M. AU - Twine, S.M. AU - Wong, A. AU - Linington, R.G. TI - Collateral sensitivity profiling in drug-resistant Escherichia coli identifies natural products suppressing cephalosporin resistance JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 14 PY - 2023 IS - 1 SN - 2041-1723 DO - 10.1038/s41467-023-37624-4 UR - https://m2.mtmt.hu/api/publication/33781796 ID - 33781796 N1 - Department of Chemistry, Simon Fraser University, 8888 University Dr., V5A 1S6, Burnaby, BC, Canada Department of Biology, Carleton University, 1125 Colonel By Dr., Ottawa, ON K1S 5B6, Canada Human Health Therapeutics Research Center, National Research Council Canada, 100 Sussex Dr., K1N 5A2, Ottawa, ON, Canada Institute for Advancing Health Through Agriculture, Texas A&M AgriLife, 1500 Research Parkway, College Station, TX 77845, United States Export Date: 28 April 2023 Correspondence Address: Linington, R.G.; Department of Chemistry, 8888 University Dr., V5A 1S6, Canada; email: rliningt@sfu.ca AB - The rapid emergence of antimicrobial resistance presents serious health challenges to the management of infectious diseases, a problem that is further exacerbated by slowing rates of antimicrobial drug discovery in recent years. The phenomenon of collateral sensitivity (CS), whereby resistance to one drug is accompanied by increased sensitivity to another, provides new opportunities to address both these challenges. Here, we present a high-throughput screening platform termed Collateral Sensitivity Profiling (CSP) to map the difference in bioactivity of large chemical libraries across 29 drug-resistant strains of E. coli. CSP screening of 80 commercial antimicrobials demonstrated multiple CS interactions. Further screening of a 6195-member natural product library revealed extensive CS relationships in nature. In particular, we report the isolation of known and new analogues of borrelidin A with potent CS activities against cephalosporin-resistant strains. Co-dosing ceftazidime with borrelidin A slows broader cephalosporin resistance with no recognizable resistance to borrelidin A itself. © 2023, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Liu, Y. AU - Gong, H. AU - Wang, Z. AU - Yuan, C. AU - Lu, J. AU - Yan, X. TI - Treatment of Superbug Infection through a Membrane-Disruption and Immune-Regulation Cascade Effect Based on Supramolecular Peptide Hydrogels JF - ADVANCED FUNCTIONAL MATERIALS J2 - ADV FUNCT MATER VL - 33 PY - 2023 IS - 45 SN - 1616-301X DO - 10.1002/adfm.202305726 UR - https://m2.mtmt.hu/api/publication/34904733 ID - 34904733 LA - English DB - MTMT ER - TY - JOUR AU - Li, W. AU - Xiao, X. AU - Qi, Y. AU - Lin, X. AU - Hu, H. AU - Shi, M. AU - Zhou, M. AU - Jiang, W. AU - Liu, L. AU - Chen, K. AU - Wang, K. AU - Liu, R. AU - Zhou, M. TI - Host-Defense-Peptide-Mimicking β-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously JF - RESEARCH J2 - RESEARCH-CHINA VL - 6 PY - 2023 SN - 2096-5168 DO - 10.34133/research.0051 UR - https://m2.mtmt.hu/api/publication/33759567 ID - 33759567 N1 - Export Date: 19 April 2023 AB - Host defense peptides (HDPs) are one of the potentially promising agents for infection diseases due to their broad spectrum and low resistance rate, but their clinical applications are limited by proteolytic instability, high-cost, and complicated synthesis process. Here, we report a host-defense-peptide-mimicking β-peptide polymer that resists proteolysis to have enhanced the activity under physiological conditions, excellent antimicrobial efficiency even at high density of bacteria, and low cost for preparation. The β-peptide polymer demonstrated quorum sensing (QS) interference and bactericidal effect against both bacterial communities and individual bacterium to simultaneously block bacterial communication and disrupt bacterial membranes. The hierarchical QS network was suppressed, and main QS signaling systems showed considerably down-regulated gene expression, resulting in excellent biofilm eradication and virulence reduction effects. The dual-modal antibacterial ability possessed excellent therapeutic effects in Pseudomonas aeruginosa pneumonia, which could inhibit biofilm formation and exhibit better antibacterial and anti-inflammatory efficiency than clinically used antibiotics, levofloxacin. Furthermore, the β-peptide polymer also showed excellent therapeutic effect Escherichia coli pyogenic liver abscess. Together, we believed that the β-peptide polymer had a feasible clinical potential to treat bacterial infection diseases. © 2023 Wanlin Li et al. LA - English DB - MTMT ER - TY - JOUR AU - Li, Wanlin AU - Xiao, Ximian AU - Qi, Yuchen AU - Lin, Xiuhui AU - Hu, Huiqun AU - Shi, Minqi AU - Zhou, Min AU - Jiang, Weinan AU - Liu, Longqiang AU - Chen, Kang AU - Wang, Kai AU - Liu, Runhui AU - Zhou, Min TI - Host-Defense-Peptide-Mimicking beta-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously JF - RESEARCH J2 - RESEARCH-CHINA VL - 2023 PY - 2023 IS - 3 SN - 2096-5168 UR - https://m2.mtmt.hu/api/publication/35297417 ID - 35297417 AB - Host defense peptides (HDPs) are one of the potentially promising agents for infection diseases due to their broad spectrum and low resistance rate,but their clinical applications are limited by proteolytic instability,high-cost,and complicated synthesis process.Here,we report a host-defense-peptidemimicking beta-peptide polymer that resists proteolysis to have enhanced the activity under physiological conditions,excellent antimicrobial efficiency even at high density of bacteria,and low cost for preparation.The beta-peptide polymer demonstrated quorum sensing (QS) interference and bactericidal effect against both bacterial communities and individual bacterium to simultaneously block bacterial communication and disrupt bacterial membranes.The hierarchical QS network was suppressed,and main QS signaling systems showed considerably down-regulated gene expression,resulting in excellent biofilm eradication and virulence reduction effects.The dual-modal antibacterial ability possessed excellent therapeutic effects in Pseudomonas aeruginosa pneumonia,which could inhibit biofilm formation and exhibit better antibacterial and anti-inflammatory efficiency than clinically used antibiotics,levofloxacin.Furthermore,the beta-peptide polymer also showed excellent therapeutic effect Escherichia coli pyogenic liver abscess.Together,we believed that the beta-peptide polymer had a feasible clinical potential to treat bacterial infection diseases. LA - English DB - MTMT ER - TY - JOUR AU - Li, Y. AU - Wang, Q. AU - Qu, X. AU - Tian, J. AU - Zhang, X. TI - Construction of palladium porphyrins and triptycene photo-activated nanomaterial for enhanced colorimetric detection and inactivation of bacteria JF - JOURNAL OF COLLOID AND INTERFACE SCIENCE J2 - J COLLOID INTERF SCI VL - 648 PY - 2023 SP - 220 EP - 230 PG - 11 SN - 0021-9797 DO - 10.1016/j.jcis.2023.05.190 UR - https://m2.mtmt.hu/api/publication/34025035 ID - 34025035 N1 - School of Chemistry and Chemical Engineering, Shandong University, Shandong, Jinan, 250100, China School of Pharmaceutical Sciences, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China Shandong Product Quality Inspection Research Institute, Shandong, Jinan, 250100, China Export Date: 20 June 2023 CODEN: JCISA Correspondence Address: Wang, Q.; School of Pharmaceutical Sciences, China; email: qbwang1@qq.com Funding details: 2019JZZY010804 Funding details: Key Technology Research and Development Program of Shandong Funding details: National Natural Science Foundation of China, NSFC, 21977064, 82100555 Funding text 1: This work was supported by the National Natural Science Foundation of China ( 21977064 and 82100555 ) and the Shandong Provincial Key Research and Development Program (the Major Scientific and Technological Innovation Project) (No. 2019JZZY010804 ). AB - In the face of increasing bacterial resistance, design of high-performing and dual-functional nanomaterials to satisfy the requirements for both detecting and eradicating bacteria is of immense importance, but still remains a great challenge. Herein, a hierarchically three-dimensional (3D) porous organic frameworks (PdPPOPHBTT) was rationally designed and fabricated for the first time to realize ideal simultaneous detection and eradication of bacteria. PdPPOPHBTT covalently integrated palladium 5,10,15,20-tetrakis-(4′-bromophenyl) porphyrin (PdTBrPP, an excellent photosensitizer) with 2,3,6,7,12,13-hexabromotriptycene (HBTT, a 3D building module). The resulting material had outstanding NIR absorption, narrow bad gap and robust singlet oxygen (1O2) production capacity, which is responsible for the sensitive detection and effective removal of bacteria. We successfully realized the colorimetric detection of S. aureus and the efficient removal of S. aureus and E. coli. The first-principles calculations found at the highly activated 1O2 derived from the 3D conjugated periodic structures and ample palladium adsorption site in PdPPOPHBTT. The bacterial infection wound model revealed that PdPPOPHBTT possesses good disinfection ability and negligible side effect to normal tissue in vivo. This finding provides an innovative strategy for designing individual porous organic polymer (POPs) with multi-function and also broaden the applications of POPs as powerful nonantibiotic type of antimicrobials. © 2023 LA - English DB - MTMT ER - TY - JOUR AU - Maasch, Jacqueline R. M. A. AU - Torres, Marcelo D. T. AU - Melo, Marcelo C. R. AU - de la Fuente-Nunez, Cesar TI - Molecular de-extinction of ancient antimicrobial peptides enabled by machine learning JF - CELL HOST & MICROBE J2 - CELL HOST MICROBE VL - 31 PY - 2023 IS - 8 SP - 1260 EP - 1274.e6 PG - 22 SN - 1931-3128 DO - 10.1016/j.chom.2023.07.001 UR - https://m2.mtmt.hu/api/publication/34617070 ID - 34617070 AB - Molecular de-extinction could offer avenues for drug discovery by reintroducing bioactive molecules that are no longer encoded by extant organisms. To prospect for antimicrobial peptides encrypted within extinct and extant human proteins, we introduce the panCleave random forest model for proteome-wide cleavage site prediction. Our model outperformed multiple protease-specific cleavage site classifiers for three modern human caspases, despite its pan-protease design. Antimicrobial activity was observed in vitro for modern and archaic protein fragments identified with panCleave. Lead peptides showed resistance to proteolysis and exhibited variable membrane permeabilization. Additionally, representative modern and archaic protein fragments showed anti-infective efficacy against A. baumannii in both a skin abscess infection model and a pre clinical murine thigh infection model. These results suggest that machine-learning-based encrypted peptide prospection can identify stable, nontoxic peptide antibiotics. Moreover, we establish molecular de-extinction through paleoproteome mining as a framework for antibacterial drug discovery. LA - English DB - MTMT ER - TY - JOUR AU - Miele, L. AU - Evans, R.M.L. AU - Cunniffe, N.J. AU - Torres-Barceló, C. AU - Bevacqua, D. TI - Evolutionary epidemiology consequences of trait-dependent control of heterogeneous parasites JF - AMERICAN NATURALIST J2 - AM NAT VL - 202 PY - 2023 IS - 5 SP - E130 EP - E146 SN - 0003-0147 DO - 10.1086/726062 UR - https://m2.mtmt.hu/api/publication/34523708 ID - 34523708 AB - Disease control can induce both demographic and evolutionary responses in host-parasite systems. Foreseeing the outcome of control therefore requires knowledge of the eco-evolutionary feedback between control and system. Previous work has assumed that control strategies have a homogeneous effect on the parasite population. However, this is not true when control targets those traits that confer to the parasite heterogeneous levels of resistance, which can additionally be related to other key parasite traits through evolutionary trade-offs. In this work, we develop aminimal model coupling epidemiological and evolutionary dynamics to explore possible trait-dependent effects of control strategies. In particular, we consider a parasite expressing continuous levels of a trait-determining resource exploitation and a control treatment that can be either positively or negatively correlated with that trait. We demonstrate the potential of trait-dependent control by considering that the decision maker may want to minimize both the damage caused by the disease and the use of treatment, due to possible environmental or economic costs. We identify efficient strategies showing that the optimal type of treatment depends on the amount applied. Our results pave the way for the study of control strategies based on evolutionary constraints, such as collateral sensitivity and resistance costs, which are receiving increasing attention for both public health and agricultural purposes. © 2023 The University of Chicago. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Montenegro-Ayo, R. AU - Pérez, T. AU - Lanza, M.R.V. AU - Brillas, E. AU - Garcia-Segura, S. AU - dos, Santos A.J. TI - New electrochemical reactor design for emergent pollutants removal by electrochemical oxidation JF - ELECTROCHIMICA ACTA J2 - ELECTROCHIM ACTA VL - 458 PY - 2023 SN - 0013-4686 DO - 10.1016/j.electacta.2023.142551 UR - https://m2.mtmt.hu/api/publication/34025036 ID - 34025036 N1 - Export Date: 20 June 2023 CODEN: ELCAA Correspondence Address: Garcia-Segura, S.; School of Sustainable Engineering and the Built Environment, United States; email: sergio.garcia.segura@asu.edu Funding details: National Science Foundation, NSF, EEC-1449500 Funding details: Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, 2014/50945-4, 2017/10118-0, 2019/20634-0, 2021/08701-4 Funding details: Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, 303759/2014-3, 303943/2021-1 Funding text 1: This work was partially funded by São Paulo research foundation (grant #2014/50945-4, #2017/10118-0, #2019/20634-0 e #2021/08701-4 ) and National Council for Scientific, Technological Development (grant #303759/2014-3 and #303943/2021-1 ) and National Science Foundation ( EEC-1449500 ) Nanosystems Engineering Research Center on Nanotechnology-Enabled Water Treatment. AB - This paper presents the theoretical and experimental confirmation of the performance of a novel pre-pilot reactor design implementing a boron-doped diamond (BDD) anode to destroy emerging pollutants by electrochemical oxidation. Turbulent flow simulation and secondary current distribution modeling with a COMSOL Multiphysics software were used to assess the engineering capabilities of the reactor along with the oxidant BDD(·OH) electrogeneration at the anode. The antibiotic ciprofloxacin (CIP) was chosen as model molecule to assess the oxidation power achieved with the pre-pilot batch plant. In sulfate medium where BDD(·OH) was the main oxidant, faster degradation was determined by increasing current density, CIP content, and pH. The effect of pH was explained by the transformation of the cationic form of CIP in acidic medium into its more easily oxidizable anionic form in alkaline medium. In chloride medium, CIP was more rapidly removed by the faster attack of the generated active chlorine. The degradation was decelerated in carbonate medium by its scavenging effect and in the presence of humic acid by its competitive oxidation with BDD(·OH). The antibiotic abatement also dropped down in tap water and synthetic urine. An almost total mineralization was achieved with a constant energy cost per unit COD mass of 0.6 ± 0.1 kWh (g COD)−1. The initial N of CIP was pre-eminently converted into nitrate, alongside nitrite and ammonia to lesser extent. Recalcitrant acetic, oxalic, and formic acids were detected as final carboxylic acids. © 2023 Elsevier Ltd LA - English DB - MTMT ER - TY - JOUR AU - Pereira, Kenzie E. AU - Deslouches, Jakobi T. AU - Deslouches, Berthony AU - Woodley, Sarah K. TI - In Vitro Investigation of the Antibacterial Activity of Salamander Skin Peptides JF - CURRENT MICROBIOLOGY J2 - CURR MICROBIOL VL - 80 PY - 2023 IS - 7 PG - 11 SN - 0343-8651 DO - 10.1007/s00284-023-03320-1 UR - https://m2.mtmt.hu/api/publication/33922528 ID - 33922528 N1 - Department of Biological Sciences, Duquesne University, Pittsburgh, PA, United States Department of Environmental and Occupational Health, School of Public Health, University of Pittsburgh, Pittsburgh, PA, United States Export Date: 6 June 2023 CODEN: CUMID Correspondence Address: Woodley, S.K.; Department of Biological Sciences, United States; email: woodleys@duq.edu Chemicals/CAS: Anti-Bacterial Agents; Anti-Infective Agents; Peptides Funding details: National Institutes of Health, NIH, RO1 GM125917 Funding details: Pittsburgh Foundation, PF Funding details: Duquesne University, DU Funding text 1: This research was supported through funding by the Samuel and Emma Winters Foundation, the Charles Henry Leach II Fund, the Pittsburgh Foundation, and Duquesne University (Community-Engaged Seed Grant Award) to SKW. It was also funded by the National Institutes of Health RO1 GM125917 to BD. Funding text 2: We thank P. Clemenza, A. Magyan, L. Rajakumar, and T. Ricketts for help in method development. We thank K. Emerson, A. Millikin, L. Muse, B. Porter, and K. Regester for help collecting amphibian skin secretions. We thank J. Klousnitzer for completing the hemolysis assays. We thank R. Yurko and K. Islam for help with the RP-HPLC. AB - Given the current and future costs of antibiotic-resistant bacteria to human health and economic productivity, there is an urgent need to develop new antimicrobial compounds. Antimicrobial peptides are a promising alternative to conventional antibiotics and other antimicrobials. Amphibian skin is a rich source of bioactive compounds, but the antibacterial properties of salamander skin peptides have been neglected. Here, we examined the in vitro ability of skin peptides from 9 species of salamander representing 6 salamander families to inhibit the growth of ESKAPE pathogens, which are bacteria that have developed resistance to conventional antibiotics. We also examined whether the skin peptides caused lysis of human red blood cells. Skin peptides from Amphiuma tridactylum had the greatest antimicrobial properties, completely inhibiting the growth of all bacterial strains except for Enterococcus faecium. Likewise, skin peptides from Cryptobranchus alleganiensis completely inhibited the growth of several of the bacterial strains. In contrast, skin peptide mixtures from Ambystoma maculatum, Desmognathus fuscus, Eurycea bislineata, E. longicauda, Necturus beyeri, N. maculosus, and Siren intermedia did not completely inhibit bacterial growth even at the highest concentrations. Finally, none of the skin peptide mixtures caused lysis of human red blood cells. Together, we demonstrate that salamander skin produces peptides with potent antibacterial properties. It remains to elucidate the peptide sequences and their antibacterial mechanisms. LA - English DB - MTMT ER - TY - JOUR AU - Pipiya, S.O. AU - Kudzhaev, A.M. AU - Mirzoeva, N.Z. AU - Mokrushina, Y.A. AU - Ziganshin, R.H. AU - Komlev, A.S. AU - Petrova, P.E. AU - Smirnov, I.V. AU - Gabibov, A.G. AU - Shamova, O.V. AU - Terekhov, S.S. TI - Bioengineering the Antimicrobial Activity of Yeast by Recombinant Thanatin Production JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 12 PY - 2023 IS - 12 SN - 2079-6382 DO - 10.3390/antibiotics12121719 UR - https://m2.mtmt.hu/api/publication/34523033 ID - 34523033 AB - The global spread of antibiotic resistance marks the end of the era of conventional antibiotics. Mankind desires new molecular tools to fight pathogenic bacteria. In this regard, the development of new antimicrobials based on antimicrobial peptides (AMPs) is again of particular interest. AMPs have various mechanisms of action on bacterial cells. Moreover, AMPs have been reported to be efficient in preclinical studies, demonstrating a low level of resistance formation. Thanatin is a small, beta-hairpin antimicrobial peptide with a bacterial-specific mode of action, predetermining its low cytotoxicity toward eukaryotic cells. This makes thanatin an exceptional candidate for new antibiotic development. Here, a microorganism was bioengineered to produce an antimicrobial agent, providing novel opportunities in antibiotic research through the directed creation of biocontrol agents. The constitutive heterologous production of recombinant thanatin (rThan) in the yeast Pichia pastoris endows the latter with antibacterial properties. Optimized expression and purification conditions enable a high production level, yielding up to 20 mg/L of rThan from the culture medium. rThan shows a wide spectrum of activity against pathogenic bacteria, similarly to its chemically synthesized analogue. The designed approach provides new avenues for AMP engineering and creating live biocontrol agents to fight antibiotic resistance. © 2023 by the authors. LA - English DB - MTMT ER - TY - JOUR AU - Ren, T. AU - Liu, J. AU - Liu, K. AU - Zhang, Z. AU - Ma, Z. AU - Dan, S.F. AU - Lan, Z. AU - Lu, M. AU - Fang, H. AU - Zhang, Y. AU - Zhu, P. AU - Liao, Y. TI - Cloning and expression of two anti-lipopolysaccharide factors in Eriocheir hepuensis under Vibrio alginolyticus-induced stress JF - JOURNAL OF FISH BIOLOGY J2 - J FISH BIOL VL - 102 PY - 2023 IS - 2 SP - 349 EP - 357 PG - 9 SN - 0022-1112 DO - 10.1111/jfb.15261 UR - https://m2.mtmt.hu/api/publication/34837559 ID - 34837559 LA - English DB - MTMT ER - TY - JOUR AU - Sanz-Garcia, Fernando AU - Gil-Gil, Teresa AU - Laborda, Pablo AU - Blanco, Paula AU - Ochoa-Sanchez, Luz-Edith AU - Baquero, Fernando AU - Martinez, Jose Luis AU - Hernando-Amado, Sara TI - Translating eco-evolutionary biology into therapy to tackle antibiotic resistance JF - NATURE REVIEWS MICROBIOLOGY J2 - NAT REV MICROBIOL VL - 21 PY - 2023 IS - 10 SP - 671 EP - 685 PG - 15 SN - 1740-1526 DO - 10.1038/s41579-023-00902-5 UR - https://m2.mtmt.hu/api/publication/33995750 ID - 33995750 N1 - Cited By :3 Export Date: 5 September 2023 CODEN: NRMAC Correspondence Address: Martínez, J.L.; Centro Nacional de Biotecnología, Spain; email: jlmtnez@cnb.csic.es Correspondence Address: Hernando-Amado, S.; Centro Nacional de Biotecnología, Spain; email: shernando@cnb.csic.es Funding details: Ministerio de Economía y Competitividad, MINECO Funding details: Ministerio de Ciencia e Innovación, MICINN Funding details: Agencia Estatal de Investigación, AEI, MCIN/AEI/10.13039/501100011033, PID2020-113521RB-I00 Funding text 1: Work in the authors’ laboratories was supported by Ministerio de Ciencia e Innovación (MCIN), Agencia Estatal de Investigación (AEI) MCIN/AEI/10.13039/501100011033 grant PID2020-113521RB-I00. T.G.-G. is recipient of a Formación de Personal Investigador fellowship from Ministerio de Economía y Competitividad. AB - In this Review, Martinez, Hernando-Amado and colleagues explore how eco-evolutionary-based approaches can aid in the design of successful antibiotic therapies, including the exploitation of evolutionary trade-offs, targeting bacterial metabolism and studying the ancestral functions of antibiotic resistance determinants.Antibiotic resistance is currently one of the most important public health problems. The golden age of antibiotic discovery ended decades ago, and new approaches are urgently needed. Therefore, preserving the efficacy of the antibiotics currently in use and developing compounds and strategies that specifically target antibiotic-resistant pathogens is critical. The identification of robust trends of antibiotic resistance evolution and of its associated trade-offs, such as collateral sensitivity or fitness costs, is invaluable for the design of rational evolution-based, ecology-based treatment approaches. In this Review, we discuss these evolutionary trade-offs and how such knowledge can aid in informing combination or alternating antibiotic therapies against bacterial infections. In addition, we discuss how targeting bacterial metabolism can enhance drug activity and impair antibiotic resistance evolution. Finally, we explore how an improved understanding of the original physiological function of antibiotic resistance determinants, which have evolved to reach clinical resistance after a process of historical contingency, may help to tackle antibiotic resistance. LA - English DB - MTMT ER - TY - JOUR AU - Savitskaya, A. AU - Masso-Silva, J. AU - Haddaoui, I. AU - Enany, S. TI - Exploring the arsenal of antimicrobial peptides: Mechanisms, diversity, and applications JF - BIOCHIMIE J2 - BIOCHIMIE VL - 214 PY - 2023 SP - 216 EP - 227 PG - 12 SN - 0300-9084 DO - 10.1016/j.biochi.2023.07.016 UR - https://m2.mtmt.hu/api/publication/34480007 ID - 34480007 N1 - Institute of Bioorganic Chemistry of Russian Academy of Science, Moscow, Russian Federation Division of Pulmonary, Critical Care, Sleep Medicine and Physiology, University of California San Diego, La Jolla, CA, United States National Research Institute of Rural Engineering, Water and Forestry, University of Carthage, LR Valorization of Unconventional Waters, Ariana, Tunisia Microbiology and Immunology Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt Biomedical Research Department, Armed Force College of Medicine, Cairo, Egypt Export Date: 05 January 2024; Cited By: 0; Correspondence Address: S. Enany; Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt; email: shymaa21@yahoo.com; CODEN: BICMB LA - English DB - MTMT ER - TY - JOUR AU - Sun, Changfa AU - Liu, Wenjie AU - Wang, Lili AU - Meng, Run AU - Deng, Jia AU - Qing, Rui AU - Wang, Bochu AU - Hao, Shilei TI - Photopolymerized keratin-PGLa hydrogels for antibiotic resistance reversal and enhancement of infectious wound healing JF - MATERIALS TODAY BIO J2 - MATER TODAY BIO VL - 23 PY - 2023 SN - 2590-0064 DO - 10.1016/j.mtbio.2023.100807 UR - https://m2.mtmt.hu/api/publication/34524545 ID - 34524545 LA - English DB - MTMT ER - TY - JOUR AU - Tang, S. W. AU - Kwok, S. H. AU - Li, X. AU - Tang, K. H. AU - Kubi, J. A. AU - Brah, A. S. AU - Yeung, K. AU - Dong, M. AU - Lam, Y. M. TI - A new class of antimicrobial therapeutics targeting the envelope stress response of Gram-negative bacteria: abridged publication JF - HONG KONG MEDICAL JOURNAL J2 - HONG KONG MED J VL - 29 PY - 2023 IS - 4 SP - 39 EP - 44 PG - 6 SN - 1024-2708 UR - https://m2.mtmt.hu/api/publication/34963046 ID - 34963046 N1 - Supplement: 4 Összes idézések száma a WoS-ban: 0 LA - English DB - MTMT ER - TY - JOUR AU - Waller, N.J.E. AU - Cheung, C.-Y. AU - Cook, G.M. AU - McNeil, M.B. TI - The evolution of antibiotic resistance is associated with collateral drug phenotypes in Mycobacterium tuberculosis JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 14 PY - 2023 IS - 1 SN - 2041-1723 DO - 10.1038/s41467-023-37184-7 UR - https://m2.mtmt.hu/api/publication/33759560 ID - 33759560 N1 - Export Date: 19 April 2023 AB - The increasing incidence of drug resistance in Mycobacterium tuberculosis has diminished the efficacy of almost all available antibiotics, complicating efforts to combat the spread of this global health burden. Alongside the development of new drugs, optimised drug combinations are needed to improve treatment success and prevent the further spread of antibiotic resistance. Typically, antibiotic resistance leads to reduced sensitivity, yet in some cases the evolution of drug resistance can lead to enhanced sensitivity to unrelated drugs. This phenomenon of collateral sensitivity is largely unexplored in M. tuberculosis but has the potential to identify alternative therapeutic strategies to combat drug-resistant strains that are unresponsive to current treatments. Here, by using drug susceptibility profiling, genomics and evolutionary studies we provide evidence for the existence of collateral drug sensitivities in an isogenic collection M. tuberculosis drug-resistant strains. Furthermore, in proof-of-concept studies, we demonstrate how collateral drug phenotypes can be exploited to select against and prevent the emergence of drug-resistant strains. This study highlights that the evolution of drug resistance in M. tuberculosis leads to collateral drug responses that can be exploited to design improved drug regimens. © 2023, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Wang, Y. AU - Wang, L. AU - Li, C. AU - Pei, Y. AU - Liu, X. AU - Tian, Y. TI - AMP-EBiLSTM: employing novel deep learning strategies for the accurate prediction of antimicrobial peptides JF - FRONTIERS IN GENETICS J2 - FRONT GENET VL - 14 PY - 2023 SN - 1664-8021 DO - 10.3389/fgene.2023.1232117 UR - https://m2.mtmt.hu/api/publication/34126800 ID - 34126800 N1 - School of Modern Post (School of Automation), Beijing University of Posts and Telecommunications, Beijing, China School of Clinical Medicine, Tsinghua University, Beijing, China Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China Vascular Surgery Department, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, China Export Date: 5 September 2023 Correspondence Address: Liu, X.; Laboratory Medicine, China; email: liuxiaoxiao@gdph.org.cn Correspondence Address: Tian, Y.; Vascular Surgery Department, China; email: tianyu21@mails.tsinghua.edu.cn Funding details: National Natural Science Foundation of China, NSFC, 32170160 Funding text 1: This study was funded by Shanxi Province “136 Revitalization Medical Project Construction Funds” and the National Natural Science Foundation of China (32170160). AB - Antimicrobial peptides are present ubiquitously in intra- and extra-biological environments and display considerable antibacterial and antifungal activities. Clinically, it has shown good antibacterial effect in the treatment of diabetic foot and its complications. However, the discovery and screening of antimicrobial peptides primarily rely on wet lab experiments, which are inefficient. This study endeavors to create a precise and efficient method of predicting antimicrobial peptides by incorporating novel machine learning technologies. We proposed a deep learning strategy named AMP-EBiLSTM to accurately predict them, and compared its performance with ensemble learning and baseline models. We utilized Binary Profile Feature (BPF) and Pseudo Amino Acid Composition (PSEAAC) for effective local sequence capture and amino acid information extraction, respectively, in deep learning and ensemble learning. Each model was cross-validated and externally tested independently. The results demonstrate that the Enhanced Bi-directional Long Short-Term Memory (EBiLSTM) deep learning model outperformed others with an accuracy of 92.39% and AUC value of 0.9771 on the test set. On the other hand, the ensemble learning models demonstrated cost-effectiveness in terms of training time on a T4 server equipped with 16 GB of GPU memory and 8 vCPUs, with training durations varying from 0 to 30 s. Therefore, the strategy we propose is expected to predict antimicrobial peptides more accurately in the future. Copyright © 2023 Wang, Wang, Li, Pei, Liu and Tian. LA - English DB - MTMT ER - TY - JOUR AU - Witzany, Christopher AU - Rolff, Jens AU - Regoes, Roland R. AU - Igler, Claudia TI - The pharmacokinetic–pharmacodynamic modelling framework as a tool to predict drug resistance evolution JF - MICROBIOLOGY-SGM J2 - MICROBIOL-SGM VL - 169 PY - 2023 IS - 7 SP - 001368 SN - 1350-0872 DO - 10.1099/mic.0.001368 UR - https://m2.mtmt.hu/api/publication/34124743 ID - 34124743 N1 - Institute of Integrative Biology, ETH Zurich, Zurich, Switzerland Evolutionary Biology, Institute for Biology, Freie Universität Berlin, Berlin, Germany School of Biological Sciences, University of Manchester, Manchester, United Kingdom Export Date: 6 September 2023 CODEN: MROBE Correspondence Address: Witzany, C.; Institute of Integrative Biology, Switzerland; email: chris.witzany@env.ethz.ch Correspondence Address: Igler, C.; Institute of Integrative Biology, Switzerland; email: claudia.igler@manchester.ac.uk AB - Pharmacokinetic–pharmacodynamic (PKPD) models, which describe how drug concentrations change over time and how that affects pathogen growth, have proven highly valuable in designing optimal drug treatments aimed at bacterial eradication. However, the fast rise of antimicrobial resistance calls for increased focus on an additional treatment optimization criterion: avoidance of resistance evolution. We demonstrate here how coupling PKPD and population genetics models can be used to determine treatment regimens that minimize the potential for antimicrobial resistance evolution. Importantly, the resulting modelling framework enables the assessment of resistance evolution in response to dynamic selection pressures, including changes in antimicrobial concentration and the emergence of adaptive phenotypes. Using antibiotics and antimicrobial peptides as an example, we discuss the empirical evidence and intuition behind individual model parameters. We further suggest several extensions of this framework that allow a more comprehensive and realistic prediction of bacterial escape from antimicrobials through various phenotypic and genetic mechanisms. LA - English DB - MTMT ER - TY - JOUR AU - Wu, Y. AU - Deng, S. AU - Wang, X. AU - Thunders, M. AU - Qiu, J. AU - Li, Y. TI - Discovery and Mechanism of Action of a Novel Antimicrobial Peptide from an Earthworm JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 11 PY - 2023 IS - 1 SN - 2165-0497 DO - 10.1128/spectrum.03206-22 UR - https://m2.mtmt.hu/api/publication/33688218 ID - 33688218 N1 - School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Shanghai Yangtze River Delta Eco-Environ. Change and Management Observation and Research Station, Ministry of Science and Technology, Shanghai, China Department of Pathology and Molecular Medicine, University of Otago, Wellington, New Zealand Export Date: 8 March 2023 Correspondence Address: Li, Y.; School of Agriculture and Biology, China; email: yinshengli@sjtu.edu.cn AB - The robust innate immune system of the earthworm provides a potential source of natural antimicrobial peptides (AMPs). However, the cost and high rediscovery rate of direct separation and purification limits their discovery. Genome sequencing of numerous earthworm species facilitates the discovery of new antimicrobial peptides. Through predicting potential antimicrobial peptides in the open reading frames of the Eisenia andrei genome and sequence optimization, a novel antimicrobial peptide, named EWAMP-R (RIWWSGGWRRWRW), was identified. EWAMP-R demonstrated good activity against various bacteria, including drug-resistant strains. The antibacterial mechanisms of EWAMP-R were explored through molecular simulation and wet-laboratory experiments. These experiments demonstrated that the bacterial membrane may be one of the targets of EWAMP-R but that there may be different interactions with Gram-negative and Gram-positive bacterial membranes. EWAMP-R can disrupt bacterial membrane integrity; however, at low concentrations, it appears that EWAMP-R may get through the membrane of Escherichia coli instead of damaging it directly, implying the existence of a secondary response. Gene expression studies identified that in E. coli, only the apoptosislike cell death (ALD) pathway was activated, while in Staphylococcus aureus, the MazEF pathway was also upregulated, limiting the influence of the ALD pathway. The different antimicrobial actions against Gram-positive and -negative bacteria can provide important information on the structure-activity relationship of AMPs and facilitate AMP design with higher specificity. This study identified a new source of antibacterial agents that has the potential to address the increasingly serious issue of antibiotic resistance. IMPORTANCE Drug-resistant bacteria are a great threat to public health and drive the search for new antibacterial agents. The living environment of earthworms necessitates a strong immune system, and therefore, they are potentially a rich resource of novel antibiotics. A novel AMP, EWAMP-R, with high antibacterial activity was found through in silico analysis of the Eisenia andrei genome. Molecular analysis investigating the interactions between EWAMP-R and the cell membrane demonstrated the importance of tryptophan and arginine residues to EWAMP-R activity. Additionally, the different secondary responses found between E. coli and S. aureus were in accordance with a common phenomenon where some antibacterial agents only target specific species of bacteria. These results provided useful molecular information to support further AMP research and design. Our study expands the sources of antimicrobial peptides and also helps to explain the adaptability of earthworms to their environment. © 2023 Wu et al. LA - English DB - MTMT ER - TY - JOUR AU - Xie, Ge AU - Du, Shuo AU - Huang, Qiuyi AU - Hu, Qiao AU - Bi, Duohang AU - Peng, Bolun AU - Tao, Juan AU - Zhang, Lianbin AU - Zhu, Jintao TI - When Iodine Meets Starch: On-Demand Generation of Photothermal Hydrogels for Mild-Temperature Photothermal-Chemo Disinfection JF - ACS APPLIED MATERIALS & INTERFACES J2 - ACS APPL MATER INTER VL - 15 PY - 2023 IS - 1 SP - 1914 EP - 1924 PG - 11 SN - 1944-8244 DO - 10.1021/acsami.2c19667 UR - https://m2.mtmt.hu/api/publication/35171120 ID - 35171120 AB - As an emerging antibacterial strategy, photothermal disinfection attracts increasing attention due to its advantages of high efficacy, wide pertinence, and non-drug resistance. However, the unavoidable shielding of observation by photothermal components and the possible damage to normal tissue caused by hyperthermia restrict its applications. Herein, we propose a composite hydrogel with the ability of on-demand generation of photothermal components and mild-temperature photothermal disinfection by elegantly tuning the binding and release of iodine and starch. The composite hydrogel is obtained by blending iodine-adsorbed pH-responsive ZIF-8 nanoparticles (NPs) with a starch-based hydrogel matrix. Through a convenient pH response, the composite hydrogel leverages the triple functions of iodine, which serves as a disinfectant and reacts with starch to generate a photothermal agent and color indicator, allowing photothermal- chemotherapy combined disinfection on demand. In vitro antibacterial experiments show that the composite hydrogel can respond to the acidification of the microenvironment caused by bacterial metabolism and produce corresponding color changes, realizing naked-eye observation. Meanwhile, under the combined treatment of heating/I2/Zn2+, the composite hydrogel can completely kill Escherichia coli and Staphylococcus aureus at a mild temperature of similar to 41 degrees C. This study represents a breakthrough in on-demand generation of photothermal hydrogels for mild-temperature photothermal disinfection. LA - English DB - MTMT ER - TY - JOUR AU - Yang, Longbing AU - Tian, Zhuqing AU - Zhao, Wenjing AU - Zhang, Jin AU - Tian, Chunren AU - Zhou, Luoxiong AU - Jiao, Zhenlong AU - Peng, Jian AU - Guo, Guo TI - Novel antimicrobial peptide DvAMP serves as a promising antifungal agent against Cryptococcus neoformans JF - BIOORGANIC CHEMISTRY J2 - BIOORG CHEM VL - 138 PY - 2023 SN - 0045-2068 DO - 10.1016/j.bioorg.2023.106679 UR - https://m2.mtmt.hu/api/publication/34075931 ID - 34075931 N1 - The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, China Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, 550025, China Translational Medicine Research Center, Guizhou Medical University, Guiyang, 550025, China School of Public Health, Guizhou Medical University, Guiyang, 550025, China Export Date: 5 September 2023 CODEN: BOCMB Correspondence Address: Guo, G.; Building Wuben, College Town, Gui'an New District, China; email: guoguo@gmc.edu.cn Chemicals/CAS: Antifungal Agents; Antimicrobial Peptides Funding details: 82060381 Funding details: 2022, 345 Funding text 1: This research was funded by the National Natural Science Foundation of China (82060381), Science and Technology Planning Project of Guizhou Province (ZK [2022] general project 345) and Natural Science Foundation of Guizhou Province (ZK [2022] Key Program 039). LA - English DB - MTMT ER - TY - JOUR AU - Yan, Y. AU - Li, Y. AU - Li, H. AU - Ma, X. AU - Tang, Y. AU - Yi, K. AU - Lin, X. AU - Li, J. AU - Liu, Z. TI - Antimicrobial Zeolitic Imidazolate Frameworks with Dual Mechanisms of Action JF - ACS INFECTIOUS DISEASES J2 - ACS INFECT DIS VL - 9 PY - 2023 IS - 3 SP - 507 EP - 517 PG - 11 SN - 2373-8227 DO - 10.1021/acsinfecdis.2c00496 UR - https://m2.mtmt.hu/api/publication/33759561 ID - 33759561 N1 - Export Date: 19 April 2023 AB - The horizontal transfer of drug-resistant genes and the formation of biofilm barriers have threatened the therapeutic efficacy of conventional antibiotic drugs. Development of non-antibiotic agents with high delivery efficiency through bacterial biofilms is urgently required. A pyrithione (PT)-loading zeolitic imidazolate framework (ZIF-8@PT) is synthesized to destroy biofilms and improve the sensitivity of bacteria to PT. ZIF-8@PT can target and destroy the biofilm as well as the cell membrane, promoting the intracellular delivery of PT and possibly its interaction with SmpB, a protein that could regulate the drug resistance of bacteria. ZIF-8@PT effectively suppresses abdominal infections induced by multiresistant Aeromonas veronii C4 in rodent models without systemic toxicity. ZIF-8@PT promises wide applications in treating infections caused by multidrug-resistant bacteria through a dual mechanism of action. © 2023 American Chemical Society. LA - English DB - MTMT ER - TY - GEN AU - Yu, Huajing AU - Guan, Chengjian AU - Qu, Guangzhen AU - Wei, Luyang AU - Liu, Shanshan AU - Jing, Chao AU - Yin, Danyang AU - Lin, Huajun AU - Zhang, Xiaodong AU - Rao, Quan AU - Wu, Hongwei AU - Liu, Kun AU - Wang, Dong AU - Zhang, Zhongtao AU - Guo, Wei TI - Comprehensive analysis of FZD7 in immune infiltration and prognosis of pan-cancer and validation in hepatocellular carcinoma PY - 2023 DO - 10.21203/rs.3.rs-2642508/v1 UR - https://m2.mtmt.hu/api/publication/34135046 ID - 34135046 AB - Abstract Background Frizzled 7, a G protein-coupled receptor, can regulate cell proliferation, migration, and tumorigenesis. The effect of FZD7 on prognosis and the immune microenvironment in pan-cancer remains elusive. Hence, we plan to provide insight into FZD7's role in pan-cancer. Methods FZD7 expression was analyzed by integrating RNA sequencing data from TCGA and GEO databases. Furthermore, we elucidated the correlation between FZD7 and prognosis, mutation landscape, immune infiltration, and biological function by analyzing multiple databases in pan-cancer. A prognostic risk model was constructed based on the sequencing data of HCC in the TCGA database and its validity was verified in the GEO database. In addition, reverse transcription-polymerase chain reaction assays were performed to validate FZD7 expression in HCC tumor tissues. Results Compared with adjacent noncancerous tissues, FZD7 was upregulated in several tumor tissues, especially HCC, and its elevated expression account for tumor progression and poor prognosis. Significant associations were found between FZD7 expression and TMB or MSI in several tumors. Immune infiltration analysis revealed a close link between FZD7 expression and the presence of myeloid-derived suppressor cells, T cell regulatory, as well as the cancer-associated fibroblasts. However, it negatively correlated with CD4 + Th1 cell and CD4 + Th2 infiltration. FZD7-related functional genes were successfully incorporated into the construction and validation of an HCC prognostic risk model. Conclusion Our study indicated that FZD7 is involved in regulating the progression of several tumors and immune infiltration, while it can serve as an effective prognosis biomarker in pan-cancer, especially HCC. This could shed light on tumor therapy. LA - English DB - MTMT ER - TY - JOUR AU - Bhattacharjya, S. AU - Mohid, S.A. AU - Bhunia, A. TI - Atomic-Resolution Structures and Mode of Action of Clinically Relevant Antimicrobial Peptides JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 9 SN - 1661-6596 DO - 10.3390/ijms23094558 UR - https://m2.mtmt.hu/api/publication/32850816 ID - 32850816 N1 - School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore Department of Biophysics, Bose Institute, Unified Academic Campus, Saltlake, Sector V, EN 80, Kolkata, 700091, India Export Date: 30 May 2022 Correspondence Address: Bhattacharjya, S.; School of Biological Sciences, 60 Nanyang Drive, Singapore; email: surajit@ntu.edu.sg AB - Global rise of infections and deaths caused by drug-resistant bacterial pathogens are among the unmet medical needs. In an age of drying pipeline of novel antibiotics to treat bacterial infections, antimicrobial peptides (AMPs) are proven to be valid therapeutics modalities. Direct in vivo applications of many AMPs could be challenging; however, works are demonstrating encouraging results for some of them. In this review article, we discussed 3-D structures of potent AMPs e.g., polymyxin, thanatin, MSI, protegrin, OMPTA in complex with bacterial targets and their mode of actions. Studies on human peptide LL37 and de novo-designed peptides are also discussed. We have focused on AMPs which are effective against drug-resistant Gram-negative bacteria. Since treatment options for the infections caused by super bugs of Gram-negative bacteria are now extremely limited. We also summarize some of the pertinent challenges in the field of clinical trials of AMPs. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Bhaumik, Kaushik Nath AU - Hetényi, Anasztázia AU - Olajos, Gábor AU - Martins, Ana AU - Spohn, Reka AU - Németh, Lukács AU - Jójárt, Balázs AU - Szili, Petra AU - Dunai, Anett AU - Jangir, Pramod K. AU - Daruka, Leila AU - Földesi, Imre AU - Kata, Diána AU - Pal, Csaba AU - Martinek, Tamás TI - Novel foldameric peptide adjuvants counter antimicrobial resistance via promoting membrane hyperpolarization JF - JOURNAL OF PEPTIDE SCIENCE J2 - J PEPT SCI VL - 28 PY - 2022 IS - Suppl. 3 SN - 1075-2617 UR - https://m2.mtmt.hu/api/publication/34136805 ID - 34136805 LA - English DB - MTMT ER - TY - JOUR AU - Nath Bhaumik, Kaushik AU - Hetényi, Anasztázia AU - Olajos, Gábor AU - Martins, Ana AU - Spohn, Réka AU - Németh, Lukács AU - Jójárt, Balázs AU - Szili, Petra AU - Dunai, Anett AU - Jangir, Pramod Kumar AU - Daruka, Lejla AU - Földesi, Imre AU - Kata, Diána AU - Pál, Csaba AU - Martinek, Tamás TI - Rationally designed foldameric adjuvants enhance antibiotic efficacy via promoting membrane hyperpolarization JF - MOLECULAR SYSTEMS DESIGN & ENGINEERING J2 - MOL SYST DES ENG VL - 7 PY - 2022 IS - 1 SP - 21 EP - 33 PG - 13 SN - 2058-9689 DO - 10.1039/D1ME00118C UR - https://m2.mtmt.hu/api/publication/32493048 ID - 32493048 N1 - Funding Agency and Grant Number: European Research CouncilEuropean Research Council (ERC)European Commission [H2020-ERC-2014-CoG 648364, H2020-ERC-2019-PoC 862077]; ELKH Lendulet Programme [LP-2017-10/2020]; National Research, Development and Innovation Office, HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [KKP 126506]; National Laboratory of Biotechnology Grant [NKFIH-871-3/2020, GINOP-2.3.2-15-2016-00014, GINOP-2.3.2-15-2016-00020]; NKFINational Research, Development & Innovation Office (NRDIO) - Hungary [PD 116222, K134754]; Ministry of Human Capacities, Hungary [20391-3/2018/FEKUSTRAT] Funding text: The study was supported by the following research grants: European Research Council H2020-ERC-2014-CoG 648364-Resistance Evolution (CP); European Research Council H2020-ERC-2019-PoC 862077-Aware (CP), ELKH Lendulet Programme LP-2017-10/2020 (CP); 'Elvonal' Programme KKP 126506 of the National Research, Development and Innovation Office, Hungary (CP), National Laboratory of Biotechnology Grant NKFIH-871-3/2020 (CP), GINOP-2.3.2-15-2016-00014 (EVOMER) (CP, TAM), GINOP-2.3.2-15-2016-00020 (MolMedEx TUMORDNS) (CP), and NKFI PD 116222 (AM), NKFI K134754 (TAM), Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT (TAM). The authors thank Dora Bokor, PharmD, for proofreading the manuscript. AB - The negative membrane potential of bacterial cells influences crucial cellular processes. Inspired by the molecular scaffold of the antimicrobial peptide PGLa, we have developed antimicrobial foldamers with a computer-guided design strategy. The novel PGLa analogues induce sustained membrane hyperpolarization. When co-administered as an adjuvant, the resulting compounds - PGLb1 and PGLb2 - have substantially reduced the level of antibiotic resistance of multi-drug resistant Escherichia coli, Klebsiella pneumoniae and Shigella flexneri clinical isolates. The observed antibiotic potentiation was mediated by hyperpolarization of the bacterial membrane caused by the alteration of cellular ion transport. Specifically, PGLb1 and PGLb2 are selective ionophores that enhance the Goldman-Hodgkin-Katz potential across the bacterial membrane. These findings indicate that manipulating bacterial membrane electrophysiology could be a valuable tool to overcome antimicrobial resistance. LA - English DB - MTMT ER - TY - JOUR AU - Cama, J. AU - Al, Nahas K. AU - Fletcher, M. AU - Hammond, K. AU - Ryadnov, M.G. AU - Keyser, U.F. AU - Pagliara, S. TI - An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 12 PY - 2022 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-022-07973-z UR - https://m2.mtmt.hu/api/publication/32850855 ID - 32850855 N1 - Export Date: 30 May 2022 Correspondence Address: Cama, J.; Living Systems Institute, Stocker Road, United Kingdom; email: camajehangir@gmail.com Correspondence Address: Pagliara, S.; Living Systems Institute, Stocker Road, United Kingdom; email: s.pagliara@exeter.ac.uk AB - Antimicrobial resistance challenges the ability of modern medicine to contain infections. Given the dire need for new antimicrobials, polypeptide antibiotics hold particular promise. These agents hit multiple targets in bacteria starting with their most exposed regions—their membranes. However, suitable approaches to quantify the efficacy of polypeptide antibiotics at the membrane and cellular level have been lacking. Here, we employ two complementary microfluidic platforms to probe the structure–activity relationships of two experimental series of polypeptide antibiotics. We reveal strong correlations between each peptide’s physicochemical activity at the membrane level and biological activity at the cellular level. We achieve this knowledge by assaying the membranolytic activities of the compounds on hundreds of individual giant lipid vesicles, and by quantifying phenotypic responses within clonal bacterial populations with single-cell resolution. Our strategy proved capable of detecting differential responses for peptides with single amino acid substitutions between them, and can accelerate the rational design and development of peptide antimicrobials. © 2022, The Author(s). LA - English DB - MTMT ER - TY - CHAP AU - Chen, Haixia AU - Li, Shuqin ED - Panichayupakaranant, Pharkphoom ED - Shirkhedkar, Atul ED - Sindhu, Rakesh K. ED - Singh, Inderbir TI - Herbal Antibiotics for Treating Drug‐Resistant Bacteria T2 - Herbal Drugs for the Management of Infectious Diseases PB - Wiley SN - 9781119818779 PY - 2022 SP - 23 EP - 53 PG - 31 DO - 10.1002/9781119818779.ch2 UR - https://m2.mtmt.hu/api/publication/34075837 ID - 34075837 LA - English DB - MTMT ER - TY - JOUR AU - Chen, X. AU - Han, J. AU - Cai, X. AU - Wang, S. TI - Antimicrobial peptides: Sustainable application informed by evolutionary constraints JF - BIOTECHNOLOGY ADVANCES J2 - BIOTECHNOL ADV VL - 60 PY - 2022 SN - 0734-9750 DO - 10.1016/j.biotechadv.2022.108012 UR - https://m2.mtmt.hu/api/publication/33030708 ID - 33030708 N1 - College of Chemical Engineering, Fuzhou University, Fujian, Fuzhou, 350108, China College of Biological Science and Engineering, Fuzhou University, Fujian, Fuzhou, 350108, China Export Date: 28 July 2022 CODEN: BIADD Correspondence Address: Wang, S.; College of Biological Science and Engineering, Fujian, China; email: shywang@fzu.edu.cn LA - English DB - MTMT ER - TY - JOUR AU - Chen, X. AU - Han, J. AU - Wang, S. TI - Integrated evolutionary analysis reveals the resistance risk to antimicrobial peptides in Staphylococcus aureus JF - FOOD CONTROL J2 - FOOD CONTROL VL - 138 PY - 2022 SN - 0956-7135 DO - 10.1016/j.foodcont.2022.108966 UR - https://m2.mtmt.hu/api/publication/32850813 ID - 32850813 N1 - College of Chemical Engineering, China College of Biological Science and Engineering, Fuzhou University, Fujian, Fuzhou, 350108, China Export Date: 30 May 2022 CODEN: FOOCE Correspondence Address: Wang, S.; College of Biological Science and Engineering, Fujian, China; email: shywang@fzu.edu.cn AB - The safety risks of antimicrobial peptide (AMP) resistance have never been properly understood. Herein, we comprehensively investigated the evolution of resistance to AMPs in Staphylococcus aureus using piscidin1 (PIS-1) and piscidin3 (PIS-3) as archetypes, in which they were both combined with cell membranes via a common structural motif but vary in permeabilizing actions. The results demonstrated that the bacterial strain acquired limited resistance to PIS-3 compared to PIS-1. However, the PIS-3-induced strain developed parlous co-resistance toward PIS-1, ampicillin, ofloxacin, rifampicin, tetracycline, vancomycin, and polymyxin B. Based on the results of the chemiluminescence method, transcriptome sequencing and proteomic analysis, the generation of bacterial co-resistance was affiliated with decreased cell membrane permeability, mainly involving the regulation of the two-component system, ATP-binding cassette transporters and phosphotransferase system. These findings highlight concerns that AMPs could trigger a new crisis of drug resistance in food packaging, feed additives, and the agricultural environment. © 2022 Elsevier Ltd LA - English DB - MTMT ER - TY - THES AU - Dermody, Roslyn TI - Nontuberculous Mycobacteria, Nontraditional Treatment PY - 2022 SN - 9798363511028 UR - https://m2.mtmt.hu/api/publication/34902055 ID - 34902055 LA - English DB - MTMT ER - TY - JOUR AU - Fang, Shanfang AU - Dang, Yuan-Ye AU - Li, Haizhou AU - Li, Hongxia AU - Liu, Jiayong AU - Zhong, Rongcui AU - Chen, Yongzhi AU - Liu, Shouping AU - Lin, Shuimu TI - Membrane-Active Antibacterial Agents Based on Calix[4]arene Derivatives: Synthesis and Biological Evaluation JF - FRONTIERS IN CHEMISTRY J2 - FRONT CHEM VL - 10 PY - 2022 SN - 2296-2646 DO - 10.3389/fchem.2022.816741 UR - https://m2.mtmt.hu/api/publication/32734830 ID - 32734830 N1 - Funding Agency and Grant Number: National Natural Science Foundation of China [21907019]; Talent Fund for High-Level University Construction of Guangzhou [B195002009029, B195002009030]; Educational Commission of Guangdong Province [2019KQNCX118]; Medical Scientific Research Foundation of Guangdong Province [A2020396]; College Student Laboratory Opening Project of Guangzhou Medical University [01-408-2102053]; High-level University Construction Fund of Guangdong Province [06-410-2107207, 06-410-2107286] Funding text: Funding This work was supported by the National Natural Science Foundation of China (21907019), the Talent Fund for High-Level University Construction of Guangzhou (B195002009029 and B195002009030), Educational Commission of Guangdong Province (2019KQNCX118), Medical Scientific Research Foundation of Guangdong Province (A2020396), College Student Laboratory Opening Project of Guangzhou Medical University (01-408-2102053), and High-level University Construction Fund of Guangdong Province (06-410-2107207; 06-410-2107286). LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Méhi, Orsolya Katinka AU - Brivio, Maurizio TI - Editorial: Antimicrobial peptides and mRNA therapy: Clinical, Veterinary, and plant pathology perspectives with special attention to combatting MDR pathogens JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 13 PY - 2022 SN - 1664-302X DO - 10.3389/fmicb.2022.1030874 UR - https://m2.mtmt.hu/api/publication/33135935 ID - 33135935 N1 - Export Date: 27 January 2023 LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Gualtieri, Maxime AU - Zeller, Matthias AU - Tarasco, Eustachio AU - Klein, Michael G. AU - Fodor, Andrea M. AU - Haynes, Leroy AU - Lengyel, Katalin AU - Forst, Steven A. AU - Furgani, Ghazala M. AU - Karaffa, Levente AU - Vellai, Tibor TI - Type Strains of Entomopathogenic Nematode-Symbiotic Bacterium Species, Xenorhabdus szentirmaii (EMC) and X. budapestensis (EMA), Are Exceptional Sources of Non-Ribosomal Templated, Large-Target-Spectral, Thermotolerant-Antimicrobial Peptides (by Both), and Iodinin (by EMC) JF - PATHOGENS J2 - PATHOGENS VL - 11 PY - 2022 IS - 3 PG - 29 SN - 2076-0817 DO - 10.3390/pathogens11030342 UR - https://m2.mtmt.hu/api/publication/32742483 ID - 32742483 N1 - Department of Genetics, Eötvös University, Pázmány Péter Sétány 1/C, Budapest, H-1117, Hungary Department of Genetics, University of Szeged, Középfasor 52, Szeged, H-6726, Hungary Nosopharm, Espace Innovation 2, 110 Allée Charles Babbage, Nîmes, 30000, France Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, United States Department of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, Via Amendola 165/A, Bari, 70126, Italy Institute for Sustainable Plant Protection of CNR, Via Amendola 122/D, Bari, 70126, Italy USDA-ARS & Department of Entomology, The Ohio State University, 13416 Claremont Ave. Cleveland, Columbus, OH 44130, United States Department of Chemistry, The College of Wooster, Wooster, OH 44691, United States National Institute of Pharmacy and Nutrition (NIPN), Zrinyi utca 3, Budapest, H-1051, Hungary Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201, United States Department of Plant Protection, Faculty of Agriculture, University of Tripoli, P.O. Box 13793, Tripoli, Libyan Arab Jamahiriya Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Egyetem Tér 1, Debrecen, H-4032, Hungary Institute of Metagenomics, University of Debrecen, Egyetem tér 1, Debrecen, H-4032, Hungary MTA-ELTE Genetics Research Group, Pázmány Péter Sétány 1/C, Budapest, H-1117, Hungary Export Date: 24 May 2022 Correspondence Address: Fodor, A.; Department of Genetics, Pázmány Péter Sétány 1/C, Hungary; email: fodorandras@yahoo.com AB - Antimicrobial multidrug resistance (MDR) is a global challenge, not only for public health, but also for sustainable agriculture. Antibiotics used in humans should be ruled out for use in veterinary or agricultural settings. Applying antimicrobial peptide (AMP) molecules, produced by soil-born organisms for protecting (soil-born) plants, seems a preferable alternative. The natural role of peptide-antimicrobials, produced by the prokaryotic partner of entomopathogenic-nematode/bacterium (EPN/EPB) symbiotic associations, is to sustain monoxenic conditions for the EPB in the gut of the semi-anabiotic infective dauer juvenile (IJ) EPN. They keep pathobiome conditions balanced for the EPN/EPB complex in polyxenic (soil, vanquished insect cadaver) niches. Xenorhabdus szentirmaii DSM16338(T) (EMC), and X. budapestensis DSM16342(T) (EMA), are the respective natural symbionts of EPN species Steinernema rarum and S. bicornutum. We identified and characterized both of these 15 years ago. The functional annotation of the draft genome of EMC revealed 71 genes encoding non-ribosomal peptide synthases, and polyketide synthases. The large spatial Xenorhabdus AMP (fabclavine), was discovered in EMA, and its biosynthetic pathway in EMC. The AMPs produced by EMA and EMC are promising candidates for controlling MDR prokaryotic and eukaryotic pathogens (bacteria, oomycetes, fungi, protozoa). EMC releases large quantity of iodinin (1,6-dihydroxyphenazine 5,10-dioxide) in a water-soluble form into the media, where it condenses to form spectacular water-insoluble, macroscopic crystals. This review evaluates the scientific impact of international research on EMA and EMC. LA - English DB - MTMT ER - TY - THES AU - Fonseca Fernández, Angie Lorena TI - Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C. PY - 2022 SP - 62 UR - https://m2.mtmt.hu/api/publication/34075909 ID - 34075909 LA - Spanish DB - MTMT ER - TY - JOUR AU - Georgieva, Maria AU - Heinonen, Tytti AU - Hargraves, Simone AU - Pillonel, Trestan AU - Widmann, Christian AU - Jacquier, Nicolas TI - The EnvZ/OmpR Two-Component System Regulates the Antimicrobial Activity of TAT-RasGAP(317-326) and the Collateral Sensitivity to Other Antibacterial Agents JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 10 PY - 2022 IS - 3 PG - 16 SN - 2165-0497 DO - 10.1128/spectrum.02009-21 UR - https://m2.mtmt.hu/api/publication/32868164 ID - 32868164 N1 - Funding Agency and Grant Number: Faculty of Biology and Medicine of the University of Lausanne Funding text: We thank Sebastien Aeby and Jean Daraspe for technical support, the genomic team of the IMUL, led by Claire Bertelli for sequencing services, Valentin Scherz for support in bioinformatics analyses, Patrick Viollier from University of Geneva, Switzerland and Linus Sandegren from Uppsala University, Sweden for providing strains, and Gilbert Greub, Institute of Microbiology, Lausanne for sharing equipment and laboratory space. This study was supported by an interdisciplinary grant of the Faculty of Biology and Medicine of the University of Lausanne. AB - Antimicrobial peptides (AMP) are promising alternatives to classical antibiotics in the fight against antibiotic resistance. Resistance toward antimicrobial peptides can occur, but little is known about the mechanisms driving this phenomenon. The rapid emergence of antibiotic-resistant bacteria poses a serious threat to public health worldwide. Antimicrobial peptides (AMPs) are promising antibiotic alternatives; however, little is known about bacterial mechanisms of AMP resistance and the interplay between AMP resistance and the bacterial response to other antimicrobials. In this study, we identified Escherichia coli mutants resistant to the TAT-RasGAP(317-326) antimicrobial peptide and found that resistant bacteria show collateral sensitivity to other AMPs and antibacterial agents. We determined that resistance to TAT-RasGAP(317-326) peptide arises through mutations in the histidine kinase EnvZ, a member of the EnvZ/OmpR two-component system responsible for osmoregulation in E. coli. In particular, we found that TAT-RasGAP(317-326) binding and entry is compromised in E. coli peptide-resistant mutants. We showed that peptide resistance is associated with transcriptional regulation of a number of pathways and EnvZ-mediated resistance is dependent on the OmpR response regulator but is independent of the OmpC and OmpF outer membrane porins. Our findings provide insight into the bacterial mechanisms of TAT-RasGAP(317-326) resistance and demonstrate that resistance to this AMP is associated with collateral sensitivity to other antibacterial agents. IMPORTANCE Antimicrobial peptides (AMP) are promising alternatives to classical antibiotics in the fight against antibiotic resistance. Resistance toward antimicrobial peptides can occur, but little is known about the mechanisms driving this phenomenon. Moreover, there is limited knowledge on how AMP resistance relates to the bacterial response to other antimicrobial agents. Here, we address these questions in the context of the antimicrobial peptide TAT-RasGAP(317-326). We show that resistant Escherichia coli strains can be selected and do not show resistance to other antimicrobial agents. Resistance is caused by a mutation in a regulatory pathway, which lowers binding and entry of the peptide in E. coli. Our results highlight a mechanism of resistance that is specific to TAT-RasGAP(317-326). Further research is required to characterize these mechanisms and to evaluate the potential of antimicrobial combinations to curb the development of antimicrobial resistance. LA - English DB - MTMT ER - TY - JOUR AU - Ghimire, J. AU - Guha, S. AU - Nelson, B.J. AU - Morici, L.A. AU - Wimley, W.C. TI - The Remarkable Innate Resistance of Burkholderia bacteria to Cationic Antimicrobial Peptides: Insights into the Mechanism of AMP Resistance JF - JOURNAL OF MEMBRANE BIOLOGY J2 - J MEMBRANE BIOL VL - 255 PY - 2022 SP - 503 EP - 511 PG - 9 SN - 0022-2631 DO - 10.1007/s00232-022-00232-2 UR - https://m2.mtmt.hu/api/publication/32850866 ID - 32850866 N1 - Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, LA, New Orleans, 70112, France Department of Microbiology and Immunology, Tulane University School of Medicine, LA, New Orleans, 70112, France Cited By :2 Export Date: 5 September 2023 CODEN: JMBBB Correspondence Address: Wimley, W.C.; Department of Biochemistry and Molecular Biology, LA, France; email: wwimley@tulane.edu Chemicals/CAS: adenosine phosphate, 61-19-8, 8063-98-7; lipid A, 95991-05-2; phosphate, 14066-19-4, 14265-44-2; Adenosine Monophosphate; Anti-Bacterial Agents; Antimicrobial Cationic Peptides; Antimicrobial Peptides; Lipid A; Lipopolysaccharides; Membrane Lipids; Phosphates AB - Gram-negative bacteria belonging to the genus Burkholderia are remarkably resistant to broad-spectrum, cationic, antimicrobial peptides (AMPs). It has been proposed that this innate resistance is related to changes in the outer membrane lipopolysaccharide (OM LPS), including the constitutive, essential modification of outer membrane Lipid A phosphate groups with cationic 4-amino-4-deoxy-arabinose. This modification reduces the overall negative charge on the OM LPS which may change the OM structure and reduce the binding, accumulation, and permeation of cationic AMPs. Similarly, the Gram-negative pathogen Pseudomonas aeruginosa can quickly become resistant to many AMPs by multiple mechanisms, frequently, including activation of the arn operon, which leads, transiently, to the same modification of Lipid A. We recently discovered a set of synthetically evolved AMPs that do not invoke any resistance in P. aeruginosa over multiple passages and thus are apparently not inhibited by aminorabinosylation of Lipid A in P. aeruginosa. Here we test these resistance-avoiding peptides, within a set of 18 potent AMPs, against Burkholderia thailandensis. We find that none of the AMPs tested have measurable activity against B. thailandensis. Some were inactive at concentrations as high as 150 μM, despite all having sterilizing activity at ≤ 10 μM against a panel of common, human bacterial pathogens, including P. aeruginosa. We speculate that the constitutive modification of Lipid A in members of the Burkholderia genus is only part of a broader set of modifications that change the architecture of the OM to provide such remarkable levels of resistance to cationic AMPs. Graphical Abstract: [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. LA - English DB - MTMT ER - TY - JOUR AU - Gorr, S.-U. AU - Brigman, H.V. AU - Anderson, J.C. AU - Hirsch, E.B. TI - The antimicrobial peptide DGL13K is active against drug-resistant gram-negative bacteria and sub-inhibitory concentrations stimulate bacterial growth without causing resistance JF - PLOS ONE J2 - PLOS ONE VL - 17 PY - 2022 IS - 8 August SN - 1932-6203 DO - 10.1371/journal.pone.0273504 UR - https://m2.mtmt.hu/api/publication/33087033 ID - 33087033 N1 - Export Date: 9 September 2022 CODEN: POLNC AB - Antimicrobial peptides may be alternatives to traditional antibiotics with reduced bacterial resistance. The antimicrobial peptide GL13K was derived from the salivary protein BPIFA2. This study determined the relative activity of the L-and D-enantiomers of GL13K to wild-type and drug-resistant strains of three gram-negative species and against Pseudomonas aeruginosa biofilms. DGL13K displayed in vitro activity against extended-spectrum beta-lactamase (ESBL)-producing and Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (MICs 16-32 μg/ml), MDR and XDR P. aeruginosa, and XDR Acinetobacter baumannii carrying metallo-beta-lactamases (MICs 8-32 μg/ml). P. aeruginosa showed low inherent resistance to DGL13K and the increased metabolic activity and growth caused by sub-MIC concentrations of GL13K peptides did not result in acquired bacterial resistance. Daily treatment for approximately two weeks did not increase the MIC of DGL13K or cause cross-resistance between LGL13K and DGL13K. These data suggest that DGL13K is a promising antimicrobial peptide candidate for further development. © 2022 Gorr et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. LA - English DB - MTMT ER - TY - JOUR AU - Guo, M. AU - Liu, D. AU - Chen, X. AU - Wu, Y. AU - Zhang, X. TI - Pathogenicity and innate response to Avibacterium paragallinarum in chickens JF - POULTRY SCIENCE J2 - POULTRY SCI VL - 101 PY - 2022 IS - 1 SN - 0032-5791 DO - 10.1016/j.psj.2021.101523 UR - https://m2.mtmt.hu/api/publication/32598572 ID - 32598572 N1 - Jiangsu Co-Innovation Center for Prevention of Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University (JIRLAAPS), Yangzhou, Jiangsu, China Export Date: 17 January 2022 Correspondence Address: Zhang, X.; Jiangsu Co-Innovation Center for Prevention of Animal Infectious Diseases and Zoonoses, China; email: zxr@yzu.edu.cn AB - Infectious coryza (IC) is an acute infectious upper respiratory disease in chickens. Recently, the prevalence of IC has increased in China. In this study, to clarify the pathogenic mechanism and innate immune response of Avibacterium paragallinarum (A. paragallinarum), an infection experiment with A. paragallinarum was conducted. Our results showed that the whole course of IC was approximately 7 d. The clinical signs score was highest at 3 dpi and decreased from 5 dpi. A large amount of mucus and exudates was found in the infraorbital sinuses and nasal cavity. The A. paragallinarum contents in blood remained the highest, reaching 9.16 × 105 CFU/g at 5 dpi, which indicated that A. paragallinarum could rapidly invade the host, replicate in the blood and cause bacteremia. A. paragallinarum targets the upper respiratory tract. The infiltration of inflammatory cells, macrophages, and heterophilic granulocytes was only observed in the nasal cavity and infraorbital sinus. The Tlr4 and Nod1 pathways were activated and induced proinflammatory responses in chickens after infection with A. paragallinarum. The expression of Il1β and Il6 in the nasal cavity was significantly higher than that in the spleen, and it was consistent with the gross lesions and pathological changes. In particular, the expression of Il6 increased 229.07-fold at 1 dpi in the nasal cavity and increased 3.12-fold in the spleen. The high level of proinflammatory cytokines in the nasal cavity at an early stage of infection may be the main factor related to acute upper respiratory inflammation in chickens. These findings provide a reference for the occurrence and development of diseases mediated by A. paragallinarum. © 2021 The Authors LA - English DB - MTMT ER -