TY  - JOUR
AU  - Baskar, Sugumar
AU  - Bharathiraja, Pradhapsingh
AU  - Rajendra Prasad, N.
TI  - Sensitization of Multidrug Resistant Cancer Cells to Doxorubicin Using Ebselen by Disturbing Cellular Redox Status
JF  - CELL BIOCHEMISTRY AND FUNCTION
J2  - CELL BIOCHEM FUNCT
VL  - 42
PY  - 2024
IS  - 7
SN  - 0263-6484
DO  - 10.1002/cbf.4134
UR  - https://m2.mtmt.hu/api/publication/35481375
ID  - 35481375
AB  - Multidrug resistance (MDR) poses a significant problem in cancer treatment, often causing adverse effects during chemotherapy. Ebselen (Ebs), a synthetic organoselenium compound, affects cellular redox status in cancer cells. In the study, we observed that Ebs disrupted cellular redox balance and sensitized drug‐resistant cells to doxorubicin (DOX) treatment. The combination of Ebs and DOX led to increased intracellular reactive oxygen species (ROS) levels and lipid peroxidation while decreasing the activity of thioredoxin reductase (TrxR) and cellular antioxidants in drug‐resistant cells. Furthermore, this combination treatment demonstrated notable chemosensitizing effects by reducing cell viability and proliferation in MDR cells compared to DOX treatment alone. Additionally, the combination of Ebs and DOX induced DNA fragmentation and exhibited G2/M phase cell cycle arrest. Immunofluorescent analysis revealed that the Ebs and DOX combination upregulated the expression of p53 and p21, which activated the mitochondrial‐dependent apoptotic pathway. The combination treatment also enhanced the upregulation of proapoptotic markers such as Bax, Caspase‐3, ‐9, and cytochrome C, while downregulating the expression of the antiapoptotic marker Bcl‐2. Therefore, the current discoveries suggest that Ebs could be employed as a drug candidate for reversing MDR in cancer cells by regulating cellular redox homeostasis.
LA  - English
DB  - MTMT
ER  - 

TY  - CHAP
AU  - Mehraj, Safiya
AU  - Jan, Shariqa
AU  - Fatima, Kaneez
AU  - Shafi, Adil
AU  - Khan, Sameer Ullah
ED  - Khan, Sameer Ullah
ED  - Malik, Fayaz
TI  - Intracellular Compartments and Drug Resistance
T2  - Drug Resistance in Cancer: Mechanisms and Strategies
PB  - Springer Nature Singapore
CY  - Singapore
SN  - 9789819716661
PY  - 2024
SP  - 269
EP  - 295
PG  - 27
DO  - 10.1007/978-981-97-1666-1_9
UR  - https://m2.mtmt.hu/api/publication/34842264
ID  - 34842264
N1  - Chapter 9
LA  - English
DB  - MTMT
ER  -