TY  - JOUR
AU  - Pokotylo, O.
AU  - Kramar, S.
AU  - Korda, M.
TI  - IMMUNOHISTOCHEMICAL ASSESSMENT OF THE MOLECULAR HYDROGEN POTENTIAL IN COLORECTAL CANCER
JF  - Clinical Anatomy and Operative Surgery
J2  - Clinical Anatomy and Operative Surgery
VL  - 24
PY  - 2025
IS  - 1
SP  - 80
EP  - 93
PG  - 14
SN  - 1727-0847
DO  - 10.24061/1727-0847.24.1.2025.12
UR  - https://m2.mtmt.hu/api/publication/36116712
ID  - 36116712
AB  - Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality. Currently, new strategies to improve the quality of life for CRC patients are being actively explored. Molecular hydrogen has garnered attention as a biologically active molecule capable of reducing oxidative stress, modulating signaling pathways related to inflammation, proliferation, and immune cell activity, and influencing the tumor microenvironment.Aim. To investigate, using immunohistochemical studies on a rat CRC model, how water enriched with molecular hydrogen affects markers of immune cells, inflammation, proliferation, and the cell cycle.Material and Methods. Animals were divided into three groups: Group 1 – control; Group 2 – rats with CRC induced by 1,2-DMH administration for 30 weeks and given access to regular water; Group 3 – rats with induced CRC that consumed water enriched with molecular hydrogen at a concentration of 0.6 ppm. For immunohistochemical detection of target molecules in the intestinal wall, antibodies against CD4+, CD8+, CD20+, CD68+ cells, TNF-α, Ki-67, and p53 were used.Results. In the colon wall of rats with CRC, the percentage of T-helper cells (CD4+) exceeded the intact value by 11.02 times, T-killer cells (CD8+) by 3.33 times, B-lymphocytes (CD20+) by 11.41 times, and the macrophage population (CD68+) in aberrant foci increased by 11.28 times, while TNF-α expression rose by 16.72 times (p<0.001 in all cases). Under carcinogenesis conditions, the index of proliferative activity (Ki-67 exceeded the norm by 2.38 times (p<0.001)) and theintensity of the cell cycle (p53 levels were 6.85 times higher than the intact value (p<0.001)) also increased.In CRC rats consuming molecular hydrogen-enriched water, compared to uncorrected rats, a slight decrease in CD4+ and CD20+ cells and a minor increase in CD8+ cells were observed in the colon wall. The average expression levels of CD68 and TNF-α in Group 3 were significantly lower than in Group 2 by 1.33 and 1.22 times, respectively. However, no statistically significant differences in Ki-67 and p53 expression in the colon wall were found between Groups 2 and 3.Conclusion. Molecular hydrogen promotes adaptive antitumor immunity, enhances the activity of cytotoxic CD8+ cells capable of destroying tumor cells, effectively reduces inflammation (CD68+, TNF-α), but does not affect proliferation (Ki-67) or p53 accumulation. This suggests its auxiliary role in modulating the microenvironment rather than influencing core oncogenic processes in the rat CRC model.
LA  - Russian
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Song, Xian-qing
AU  - Li, Rong-jiang
AU  - Zhang, Sen
TI  - Prediction of prognosis of patients with hepatocellular carcinoma based on immune-related score
JF  - ASIAN JOURNAL OF SURGERY
J2  - ASIAN J SURG
VL  - 47
PY  - 2024
IS  - 1
SP  - 310
EP  - 319
PG  - 10
SN  - 1015-9584
DO  - 10.1016/j.asjsur.2023.08.175
UR  - https://m2.mtmt.hu/api/publication/34127935
ID  - 34127935
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Zhu, Chang
AU  - Liao, Jing-Yu
AU  - Liu, Yi-Yang
AU  - Chen, Ze-Yu
AU  - Chang, Rui-Zhi
AU  - Chen, Xiao-Ping
AU  - Zhang, Bi-Xiang
AU  - Liang, Jun-Nan
TI  - Immune dynamics shaping pre-metastatic and metastatic niches in liver metastases: from molecular mechanisms to therapeutic strategies
JF  - MOLECULAR CANCER
J2  - MOL CANCER
VL  - 23
PY  - 2024
IS  - 1
SN  - 1476-4598
DO  - 10.1186/s12943-024-02171-z
UR  - https://m2.mtmt.hu/api/publication/35575000
ID  - 35575000
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Iwamuro, Masaya
AU  - Takahashi, Takahide
AU  - Hirabata, Araki
AU  - Tanaka, Takehiro
AU  - Otsuka, Fumio
AU  - Okada, Horoyuki
TI  - Increased CCR4+ and Decreased Central Memory CD4+ T Lymphocytes in the Background Gastric Mucosa of Patients Developing Gastric Cancer After Helicobacter pylori Eradication: An Exploratory Study
JF  - CUREUS
J2  - CUREUS
VL  - 14
PY  - 2022
IS  - 11
SN  - 2168-8184
DO  - 10.7759/cureus.31713
UR  - https://m2.mtmt.hu/api/publication/33260874
ID  - 33260874
LA  - English
DB  - MTMT
ER  -