TY - JOUR AU - Kovács, Kinga Dóra AU - Szittner, Zoltán AU - Magyaródi, Beatrix AU - Péter, Beatrix AU - Szabó, Bálint AU - Vörös, Alexa AU - Kanyó, Nicolett AU - Székács, Inna AU - Horváth, Róbert TI - Optical sensor reveals the hidden influence of cell dissociation on adhesion measurements JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 10 SN - 2045-2322 DO - 10.1038/s41598-024-61485-6 UR - https://m2.mtmt.hu/api/publication/34878499 ID - 34878499 AB - Cell adhesion experiments are important in tissue engineering and for testing new biologically active surfaces, prostheses, and medical devices. Additionally, the initial state of adhesion (referred to as nascent adhesion) plays a key role and is currently being intensively researched. A critical step in handling all adherent cell types is their dissociation from their substrates for further processing. Various cell dissociation methods and reagents are used in most tissue culture laboratories (here, cell dissociation from the culture surface, cell harvesting, and cell detachment are used interchangeably). Typically, the dissociated cells are re-adhered for specific measurements or applications. However, the impact of the choice of dissociation method on cell adhesion in subsequent measurements, especially when comparing the adhesivity of various surfaces, is not well clarified. In this study, we demonstrate that the application of a label-free optical sensor can precisely quantify the effect of cell dissociation methods on cell adhesivity, both at the single-cell and population levels. The optical measurements allow for high-resolution monitoring of cellular adhesion without interfering with the physiological state of the cells. We found that the choice of reagent significantly alters cell adhesion on various surfaces. Our results clearly demonstrate that biological conclusions about cellular adhesion when comparing various surfaces are highly dependent on the employed dissociation method. Neglecting the choice of cellular dissociation can lead to misleading conclusions when evaluating cell adhesion data from various sources and comparing the adhesivity of two different surfaces (i.e., determining which surface is more or less adhesive). LA - English DB - MTMT ER - TY - JOUR AU - Péter, Beatrix AU - Székács, Inna AU - Horváth, Róbert TI - Label-free biomolecular and cellular methods in small molecule epigallocatechin-gallate research JF - HELIYON J2 - HELIYON VL - 10 PY - 2024 IS - 3 PG - 17 SN - 2405-8440 DO - 10.1016/j.heliyon.2024.e25603 UR - https://m2.mtmt.hu/api/publication/34572948 ID - 34572948 LA - English DB - MTMT ER - TY - JOUR AU - Sallai, Igor TI - Resonant Waveguide Grating Biosensors Based on Label-free Optical Principle JF - PERIODICA POLYTECHNICA-ELECTRICAL ENGINEERING AND COMPUTER SCIENCE J2 - PERIOD POLYTECH ELECTR ENG COMP SCI VL - 68 PY - 2024 IS - 1 SP - 107 EP - 114 PG - 8 SN - 2064-5260 DO - 10.3311/PPee.22878 UR - https://m2.mtmt.hu/api/publication/34186883 ID - 34186883 AB - Label-free optical biosensors are powerful tools for the real-time monitoring of both molecular and cellular-scale interactions. Resonant waveguide grating biosensors are based on the detection of refractive index changes induced by molecular interactions and/or cell mass redistributions. The Epic BenchTop and Epic Cardio are two biosensors with high sensitivity and throughput that offer excellent potential for life science research. Both instruments are suitable for cell-based and biochemical assays. In this paper, I describe the principles of operation and performance of the Epic BenchTop and Epic Cardio label-free waveguide grating biosensors and discuss their applications in various research areas. LA - English DB - MTMT ER - TY - JOUR AU - Kumar, B. AU - Chaujar, R. TI - Fin field-effect-transistor engineered sensor for detection of MDA-MB-231 breast cancer cells: A switching-ratio-based sensitivity analysis JF - PHYSICAL REVIEW E: COVERING STATISTICAL NONLINEAR BIOLOGICAL AND SOFT MATTER PHYSICS (2016-) J2 - PHYSICAL REVIEW E VL - 108 PY - 2023 IS - 3 SN - 2470-0045 DO - 10.1103/PhysRevE.108.034408 UR - https://m2.mtmt.hu/api/publication/34230636 ID - 34230636 N1 - Összes idézések száma a WoS-ban: 0 LA - English DB - MTMT ER - TY - JOUR AU - Péter, Beatrix AU - Kanyó, Nicolett AU - Kovács, Kinga Dóra AU - Kovács, Viktor AU - Székács, Inna AU - Pécz, Béla AU - Molnár, Kinga AU - Nakanishi, Hideyuki AU - Lagzi, István László AU - Horváth, Róbert TI - Glycocalyx Components Detune the Cellular Uptake of Gold Nanoparticles in a Size- and Charge-Dependent Manner JF - ACS APPLIED BIO MATERIALS J2 - ACS APPL BIO MATER VL - 6 PY - 2023 IS - 1 SP - 64 EP - 73 PG - 10 SN - 2576-6422 DO - 10.1021/acsabm.2c00595 UR - https://m2.mtmt.hu/api/publication/33152687 ID - 33152687 LA - English DB - MTMT ER - TY - JOUR AU - Richter, J.R. AU - Sanderson, R.D. TI - The glycocalyx: Pathobiology and repair JF - MATRIX BIOLOGY PLUS J2 - MATRIX BIOL PLUS VL - 17 PY - 2023 SN - 2590-0285 DO - 10.1016/j.mbplus.2023.100128 UR - https://m2.mtmt.hu/api/publication/33734726 ID - 33734726 LA - English DB - MTMT ER - TY - JOUR AU - Szittner, Zoltán AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Székács, Inna AU - Horváth, Róbert TI - Functional blood cell analysis by label-free biosensors and single-cell technologies JF - ADVANCES IN COLLOID AND INTERFACE SCIENCE J2 - ADV COLLOID INTERFAC VL - 308 PY - 2022 PG - 65 SN - 0001-8686 DO - 10.1016/j.cis.2022.102727 UR - https://m2.mtmt.hu/api/publication/33022685 ID - 33022685 LA - English DB - MTMT ER -