@article{MTMT:34878499, title = {Optical sensor reveals the hidden influence of cell dissociation on adhesion measurements}, url = {https://m2.mtmt.hu/api/publication/34878499}, author = {Kovács, Kinga Dóra and Szittner, Zoltán and Magyaródi, Beatrix and Péter, Beatrix and Szabó, Bálint and Vörös, Alexa and Kanyó, Nicolett and Székács, Inna and Horváth, Róbert}, doi = {10.1038/s41598-024-61485-6}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34878499}, abstract = {Cell adhesion experiments are important in tissue engineering and for testing new biologically active surfaces, prostheses, and medical devices. Additionally, the initial state of adhesion (referred to as nascent adhesion) plays a key role and is currently being intensively researched. A critical step in handling all adherent cell types is their dissociation from their substrates for further processing. Various cell dissociation methods and reagents are used in most tissue culture laboratories (here, cell dissociation from the culture surface, cell harvesting, and cell detachment are used interchangeably). Typically, the dissociated cells are re-adhered for specific measurements or applications. However, the impact of the choice of dissociation method on cell adhesion in subsequent measurements, especially when comparing the adhesivity of various surfaces, is not well clarified. In this study, we demonstrate that the application of a label-free optical sensor can precisely quantify the effect of cell dissociation methods on cell adhesivity, both at the single-cell and population levels. The optical measurements allow for high-resolution monitoring of cellular adhesion without interfering with the physiological state of the cells. We found that the choice of reagent significantly alters cell adhesion on various surfaces. Our results clearly demonstrate that biological conclusions about cellular adhesion when comparing various surfaces are highly dependent on the employed dissociation method. Neglecting the choice of cellular dissociation can lead to misleading conclusions when evaluating cell adhesion data from various sources and comparing the adhesivity of two different surfaces (i.e., determining which surface is more or less adhesive).}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Szabó, Bálint/0000-0002-9865-6336; Horváth, Róbert/0000-0001-8617-2302} } @article{MTMT:34572948, title = {Label-free biomolecular and cellular methods in small molecule epigallocatechin-gallate research}, url = {https://m2.mtmt.hu/api/publication/34572948}, author = {Péter, Beatrix and Székács, Inna and Horváth, Róbert}, doi = {10.1016/j.heliyon.2024.e25603}, journal-iso = {HELIYON}, journal = {HELIYON}, volume = {10}, unique-id = {34572948}, year = {2024}, eissn = {2405-8440}, orcid-numbers = {Horváth, Róbert/0000-0001-8617-2302} } @article{MTMT:34186883, title = {Resonant Waveguide Grating Biosensors Based on Label-free Optical Principle}, url = {https://m2.mtmt.hu/api/publication/34186883}, author = {Sallai, Igor}, doi = {10.3311/PPee.22878}, journal-iso = {PERIOD POLYTECH ELECTR ENG COMP SCI}, journal = {PERIODICA POLYTECHNICA-ELECTRICAL ENGINEERING AND COMPUTER SCIENCE}, volume = {68}, unique-id = {34186883}, issn = {2064-5260}, abstract = {Label-free optical biosensors are powerful tools for the real-time monitoring of both molecular and cellular-scale interactions. Resonant waveguide grating biosensors are based on the detection of refractive index changes induced by molecular interactions and/or cell mass redistributions. The Epic BenchTop and Epic Cardio are two biosensors with high sensitivity and throughput that offer excellent potential for life science research. Both instruments are suitable for cell-based and biochemical assays. In this paper, I describe the principles of operation and performance of the Epic BenchTop and Epic Cardio label-free waveguide grating biosensors and discuss their applications in various research areas.}, year = {2024}, eissn = {2064-5279}, pages = {107-114} } @article{MTMT:34230636, title = {Fin field-effect-transistor engineered sensor for detection of MDA-MB-231 breast cancer cells: A switching-ratio-based sensitivity analysis}, url = {https://m2.mtmt.hu/api/publication/34230636}, author = {Kumar, B. and Chaujar, R.}, doi = {10.1103/PhysRevE.108.034408}, journal-iso = {PHYSICAL REVIEW E}, journal = {PHYSICAL REVIEW E: COVERING STATISTICAL NONLINEAR BIOLOGICAL AND SOFT MATTER PHYSICS (2016-)}, volume = {108}, unique-id = {34230636}, issn = {2470-0045}, year = {2023}, eissn = {2470-0053} } @article{MTMT:33152687, title = {Glycocalyx Components Detune the Cellular Uptake of Gold Nanoparticles in a Size- and Charge-Dependent Manner}, url = {https://m2.mtmt.hu/api/publication/33152687}, author = {Péter, Beatrix and Kanyó, Nicolett and Kovács, Kinga Dóra and Kovács, Viktor and Székács, Inna and Pécz, Béla and Molnár, Kinga and Nakanishi, Hideyuki and Lagzi, István László and Horváth, Róbert}, doi = {10.1021/acsabm.2c00595}, journal-iso = {ACS APPL BIO MATER}, journal = {ACS APPLIED BIO MATERIALS}, volume = {6}, unique-id = {33152687}, issn = {2576-6422}, year = {2023}, eissn = {2576-6422}, pages = {64-73}, orcid-numbers = {Pécz, Béla/0000-0002-4651-6972; Molnár, Kinga/0000-0002-7196-5331; Horváth, Róbert/0000-0001-8617-2302} } @article{MTMT:33734726, title = {The glycocalyx: Pathobiology and repair}, url = {https://m2.mtmt.hu/api/publication/33734726}, author = {Richter, J.R. and Sanderson, R.D.}, doi = {10.1016/j.mbplus.2023.100128}, journal-iso = {MATRIX BIOL PLUS}, journal = {MATRIX BIOLOGY PLUS}, volume = {17}, unique-id = {33734726}, issn = {2590-0285}, year = {2023} } @article{MTMT:33022685, title = {Functional blood cell analysis by label-free biosensors and single-cell technologies}, url = {https://m2.mtmt.hu/api/publication/33022685}, author = {Szittner, Zoltán and Péter, Beatrix and Kurunczi, Sándor and Székács, Inna and Horváth, Róbert}, doi = {10.1016/j.cis.2022.102727}, journal-iso = {ADV COLLOID INTERFAC}, journal = {ADVANCES IN COLLOID AND INTERFACE SCIENCE}, volume = {308}, unique-id = {33022685}, issn = {0001-8686}, year = {2022}, eissn = {1873-3727}, orcid-numbers = {Kurunczi, Sándor/0000-0002-6567-5231; Horváth, Róbert/0000-0001-8617-2302} }