TY - JOUR AU - Lipták, Klaudia Margit AU - Lipták, Laura Mária AU - Rózsa, Noémi Katinka AU - Hermann, Péter AU - Tárnoki, Ádám Domonkos AU - Tárnoki, Dávid László AU - Végh, Dániel TI - Oral Health Knowledge and Habits of Hungarian Monozygotic and Dizygotic Twins: A Pilot Study JF - INTERNATIONAL DENTAL JOURNAL J2 - INT DENT J VL - 74 PY - 2024 IS - 1 SP - 66 EP - 70 PG - 5 SN - 0020-6539 DO - 10.1016/j.identj.2023.06.012 UR - https://m2.mtmt.hu/api/publication/34081254 ID - 34081254 AB - ABSTRACT Objective The aim of this research was to collate and analyse the data on the oral health knowledge and the related habits of a Hungarian cohort of monozygotic (MZ) and dizygotic (DZ) twins using the newly developed World Health Organisation Oral Health Questionnaire for Adults (Annex 7). Method A total of 15 sets of MZ twins and 14 sets of DZ twins (58 individuals) aged between 18 and 71 years were enrolled in the study. Each participant had to fill out a web-based questionnaire which comprised 23 questions (Google Forms). The data were collated and the oral health/hygiene habits of MZ and DZ twins were compared. Results No significant differences were detected between MZ and DZ twins with regards to their daily tooth-cleaning habits or the tooth-cleaning products used by the 2 groups. For instance, when asked how often they clean their teeth, 80% of MZ twins and 71% of DZ twins responded similarly. Further, both groups provided similar responses when questioned about the use of fluoride toothpaste, frequency of dental visits, and dental counselling received as well as a number of other parameters such as snacking of sweets and fear of visiting dentists. Conclusions Our pilot analysis of the questionnaire responses from MZ and DZ twins in Hungary did not indicate any significant differences in their oral care habits in general. Further studies with a large cohort are required to confirm or refute our findings. LA - English DB - MTMT ER - TY - JOUR AU - Ouyang, Wenbin AU - Tang, Bei AU - He, Yongmei AU - Wu, Hao AU - Yang, Pingting AU - Yin, Lu AU - Li, Xiaohui AU - Li, Ying AU - Huang, Xin TI - Mediation effect of gut microbiota on the relationship between physical activity and carotid plaque JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 15 PY - 2024 PG - 9 SN - 1664-302X DO - 10.3389/fmicb.2024.1432008 UR - https://m2.mtmt.hu/api/publication/35358121 ID - 35358121 LA - English DB - MTMT ER - TY - JOUR AU - Rashid, Safia AU - Sado, Abdulmaleek Idanesimhe AU - Afzal, Muhammad Sohaib AU - Ahmed, Amna AU - Almaalouli, Bsher AU - Waheed, Tallha AU - Abid, Rabia AU - Majumder, Koushik AU - Kumar, Vikash AU - Tejwaney, Usha AU - Kumar, Sarwan TI - Role of gut microbiota in cardiovascular diseases - a comprehensive review JF - ANNALS OF MEDICINE AND SURGERY J2 - ANN MED SURG VL - 86 PY - 2024 IS - 3 SP - 1483 EP - 1489 PG - 7 SN - 2049-0801 DO - 10.1097/MS9.0000000000001419 UR - https://m2.mtmt.hu/api/publication/34946766 ID - 34946766 LA - English DB - MTMT ER - TY - JOUR AU - Dong, Yu AU - Xu, Rui AU - Chen, Xiaowei AU - Yang, Chuanli AU - Jiang, Fei AU - Shen, Yan AU - Li, Qiong AU - Fang, Fujin AU - Li, Yongjun AU - Shen, Xiaobing TI - Characterization of gut microbiota in adults with coronary atherosclerosis JF - PEERJ J2 - PEERJ VL - 11 PY - 2023 PG - 16 SN - 2167-8359 DO - 10.7717/peerj.15245 UR - https://m2.mtmt.hu/api/publication/34349746 ID - 34349746 N1 - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, China Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing, China Department of Cardiology, Zhongda Hospital, Southeast University, Nanjing, China Cited By :1 Export Date: 12 January 2024 Correspondence Address: Li, Y.; Department of Cardiology, China; email: 2650659538@qq.com Correspondence Address: Shen, X.; Key Laboratory of Environmental Medicine Engineering, China; email: xb.shen@seu.edu.cn Tradenames: Novaseq 6000, Illumina Manufacturers: Illumina; Tiangen, China AB - Background. Cardiovascular disease, which is mainly caused by coronary atheroscle-rosis, is one of the leading causes of death and disability worldwide. Gut microbiota likely play an important role in coronary atherosclerosis. This study aims to investigate the microbiota profile of adults with coronary atherosclerosis to provide a theoretical basis for future research.Methods. Fecal samples were collected from 35 adult patients diagnosed with coronary atherosclerosis and 32 healthy adults in Nanjing, China, and the V3-V4 region of 16S rDNA genes was sequenced using high-throughput sequencing. Differences in alpha diversity, beta diversity, and gut microbiota composition between the two groups were then compared.Results. A beta diversity analysis revealed significant differences between adults with coronary atherosclerosis and controls, but there was no statistical difference in alpha diversity between the two groups. There were also differences in the composition of the gut microbiota between the two groups. The genera, Megamonas, Streptococcus, Veillonella, Ruminococcus_torques_group, Prevotella_2, Tyzzerella_4, were identified as potential biomarkers for coronary atherosclerosis.Conclusion. There are some differences in the gut microbiota of adults with coronary atherosclerosis compared to healthy adults. The insights from this study could be used to explore microbiome-based mechanisms for coronary atherosclerosis. LA - English DB - MTMT ER - TY - JOUR AU - Wang, T. AU - Xu, M. AU - Xu, C. AU - Wu, Y. AU - Dong, X. TI - Comparison of microvascular flow imaging and contrast-enhanced ultrasound for blood flow analysis of cervical lymph node lesions JF - CLINICAL HEMORHEOLOGY AND MICROCIRCULATION J2 - CLIN HEMORHEOL MICRO VL - 85 PY - 2023 IS - 3 SP - 249 EP - 259 PG - 11 SN - 1386-0291 DO - 10.3233/CH-231860 UR - https://m2.mtmt.hu/api/publication/34499729 ID - 34499729 N1 - Department of Ultrasound, The Fourth Affiliated Hospital of Harbin Medical University, Heilongjiang, Harbin, China Department of Medical Ultrasound, The Fourth Affiliated Hospital of Harbin Medical University, Heilongjiang, Harbin, 150000, China Export Date: 12 January 2024 CODEN: CHMIF Chemicals/CAS: sulfur hexafluoride, 2551-62-4; Contrast Media Tradenames: sono vue, Bracco, Italy Manufacturers: Bracco, ItalySamsung AB - OBJECTIVE: To compare the diagnostic value of microvascular flow imaging (MVFI) with that of contrast-enhanced ultrasound (CEUS) for the analysis of blood flow in benign and malignant cervical lymph nodes. MATERIAL AND METHODS: As a prospective study, 95 cervical enlarged lymph nodes (43 benign and 52 malignant) were observed in 95 patients using conventional ultrasonography (including gray and Color Doppler Flow Imaging), CEUS, and MVFI. Two researchers evaluated vascular parameters of MVFI (vascular distribution, internal vascular features, vascular index) and CEUS (enhancement mode, enhancement type) and compared the diagnostic effects of MVFI and CEUS.All results were compared with pathological findings. RESULTS: There were significant differences in the vascular distribution and internal vascular features of benign and malignant lymph nodes on MVFI (P < 0.05). The vascular distribution of benign lymph nodes was mainly of the central and avascular types, the internal blood vessels were mostly normal, the vascular distribution of malignant lymph nodes was mainly mixed, the internal vessels were mainly tortuous and displaced. The optimal cut-off value of the benign and malignant lymph node vascular index (VI) was 15.55%, and the area under the receiver operating characteristic curve (AUC) of the VI was 0.876. There were also significant differences in the enhancement mode and types of benign and malignant lymph nodes in CEUS (P < 0.05). The benign lymph nodes showed centrifugal perfusion, and the enhancement types were mostly type I and type II. Most malignant lymph nodes showed centripetal or mixed perfusion, and the enhancement types were usually type III and type IV. The accuracy, sensitivity, and specificity of CEUS in the diagnosis of lymph node lesions were 84.2%, 84.6% and 83.7%, respectively, and the AUC was 0.845. The accuracy, sensitivity, and specificity of MVFI in the diagnosis of lymph node lesions were 85.3%, 84.6%, and 86.0%, respectively, and the AUC was 0.886. CONCLUSION: Both CEUS and MVFI are valuable in differentiating benign and malignant lesions of lymph nodes and have a similar diagnostic performance; however, MVFI is less invasive and simpler than CEUS. Therefore it is preferred for auxiliary examination of enlarged lymph nodes that are difficult to diagnose by conventional ultrasound. © 2023 – IOS Press. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Bikov, András AU - Szabó, Helga AU - Piroska, Márton AU - Kunos, László AU - Szily, Marcell AU - Ligeti, Balázs AU - Makra, Nóra AU - Szabó, Dóra AU - Tárnoki, Dávid László AU - Tárnoki, Ádám Domonkos TI - Gut Microbiome in Patients with Obstructive Sleep Apnoea JF - APPLIED SCIENCES-BASEL J2 - APPL SCI-BASEL VL - 12 PY - 2022 IS - 4 PG - 12 SN - 2076-3417 DO - 10.3390/app12042007 UR - https://m2.mtmt.hu/api/publication/32687570 ID - 32687570 N1 - North West Lung Centre, Wythenshawe Hospital, Manchester University Foundation Trust, Manchester, M23 9LT, United Kingdom Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, The University of Manchester, Manchester, M13 9NT, United Kingdom Department of Pulmonology, Semmelweis University, Budapest, 1085, Hungary Medical Imaging Centre, Semmelweis University, Budapest, 1085, Hungary Central Radiological Diagnostic Department, Medical Centre Hungarian Defence Forces, Budapest, 1134, Hungary Institute of Medical Microbiology, Semmelweis University, Budapest, 1085, Hungary Faculty of Information Technology and Bionics, Pazmany Peter Catholic University, Budapest, 1085, Hungary Cited By :3 Export Date: 20 July 2023 Correspondence Address: Tarnoki, A.D.; Medical Imaging Centre, Hungary; email: tarnoki2@gmail.com LA - English DB - MTMT ER - TY - JOUR AU - Cao, Weijie AU - Xu, Yiting AU - Shen, Yun AU - Hu, Tingting AU - Wang, Yufei AU - Ma, Xiaojing AU - Bao, Yuqian TI - Neck circumference predicts development of carotid intima-media thickness and carotid plaque: A community-based longitudinal study JF - NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES J2 - NUTR METAB CARDIOVAS VL - 32 PY - 2022 IS - 7 SP - 1627 EP - 1634 PG - 8 SN - 0939-4753 DO - 10.1016/j.numecd.2022.03.027 UR - https://m2.mtmt.hu/api/publication/33368228 ID - 33368228 N1 - Export Date: 12 January 2024 Correspondence Address: Bao, Y.; Department of Endocrinology and Metabolism, China; email: yqbao@sjtu.edu.cn Correspondence Address: Ma, X.; Department of Endocrinology and Metabolism, China; email: maxiaojing@sjtu.edu.cn Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alkaline phosphatase, 9001-78-9; aspartate aminotransferase, 9000-97-9; C reactive protein, 9007-41-4; cholesterol, 57-88-5; creatinine, 19230-81-0, 60-27-5; gamma glutamyltransferase, 85876-02-4; glucose, 50-99-7, 84778-64-3; glycosylated hemoglobin, 9062-63-9; insulin, 9004-10-8 Tradenames: Voluson 730 Expert, GE Healthcare, United States Manufacturers: GE Healthcare, United States AB - Background and aims: Carotid intima-media thickness (C-IMT) is an important index for evaluating subclinical atherosclerosis. Neck circumference (NC), a new anthropometric index of the upper body fat, is closely related to cardiovascular disease (CVD) and CVD risk factors. This study investigated the relationship between NC, C-IMT, and carotid plaque in a community based cohort. Methods and results: Participants recruited from Shanghai communities were followed up for 1.1-2.9 years. All participants underwent anthropometric and biochemical measurements. Elevated NC was defined as NC > 38.5 cm and NC > 34.5 cm in men and women, respectively. Elevated CIMT, determined by ultrasound, was defined as a level higher than the 75th percentile in the study population (>0.75 mm). In total, 1189 participants without carotid plaque at baseline were included, with an average age of 59.6 +/- 7.3 years. After a mean follow-up of 2.1 +/- 0.2 years, 203 participants developed carotid plaques. After adjusting for various atherosclerosis risk factors, the logistic regression showed that the higher NC group had a significantly greater risk of developing carotid plaque than the lower NC group (odds ratio [OR], 1.55; 95% confidence interval [CI], 1.12-2.14; P = 0.008). Of those without carotid plaque at follow-up, 495 participants developed elevated C-IMT. Compared to the lower NC group, the higher NC group had a significantly increased risk of elevated C-IMT (OR, 1.49; 95% CI, 1.14-1.95; P = 0.003). Conclusion: Higher NC was significantly positively correlated with the risk of carotid plaque and elevated C-IMT. (c) 2022 The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Deng, Bing AU - Tao, Liyu AU - Wang, Yiru TI - Natural products against inflammation and atherosclerosis: Targeting on gut microbiota JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 13 PY - 2022 PG - 16 SN - 1664-302X DO - 10.3389/fmicb.2022.997056 UR - https://m2.mtmt.hu/api/publication/33919129 ID - 33919129 N1 - Export Date: 28 November 2023 AB - The gut microbiota (GM) has become recognized as a crucial element in preserving human fitness and influencing disease consequences. Commensal and pathogenic gut microorganisms are correlated with pathological progress in atherosclerosis (AS). GM may thus be a promising therapeutic target for AS. Natural products with cardioprotective qualities might improve the inflammation of AS by modulating the GM ecosystem, opening new avenues for researches and therapies. However, it is unclear what components of natural products are useful and what the actual mechanisms are. In this review, we have summarized the natural products relieving inflammation of AS by regulating the GM balance and active metabolites produced by GM. LA - English DB - MTMT ER - TY - JOUR AU - Grammatopoulos, K. AU - Antoniou, V.-D. AU - Mavrothalassitis, E. AU - Mouziouras, D. AU - Argyris, A.A. AU - Emmanouil, E. AU - Vlachopoulos, C. AU - Protogerou, A.D. TI - Association of gut microbiota composition and their metabolites with subclinical atheromatosis: A systematic review JF - AMERICAN HEART JOURNAL PLUS: CARDIOLOGY RESEARCH AND PRACTICE J2 - AM HEART J PLUS VL - 23 PY - 2022 SN - 2666-6022 DO - 10.1016/j.ahjo.2022.100219 UR - https://m2.mtmt.hu/api/publication/34499733 ID - 34499733 N1 - Cardiovascular Prevention and Research Unit, Clinic & Laboratory of Pathophysiology, Department of Medicine, Faculty of Health Sciences, National and Kapodistrian University of Athens, Greece 1st Department of Cardiology, National and Kapodistrian University of Athens, Greece Export Date: 12 January 2024 Correspondence Address: Protogerou, A.D.; Cardiovascular Prevention & Research Unit, 75, Mikras Asias Street (Building 16, 3rd floor, room 8), Greece; email: aprotog@med.uoa.gr AB - Study objective: The present systematic review investigates the hypothesis that specific components of the intestinal microbiome and/or their metabolites are associated with early stages of subclinical arterial damage (SAD). Design: Based on the MOOSE criteria, we conducted a systematic review of the literature (Scopus, Medline) investigating the potential association between gut microbiota and the most widely applied arterial biomarkers of SAD. Participants: All studies included individuals without established cardiovascular disease, either with or without SAD. Intervention: No interventions were made. Main outcome measures: Association between exposure (components/metabolites of microbiota) and outcome (presence of SAD). Results: Fourteen articles met the predefined criteria. Due to the large heterogeneity, their meta-analysis was not possible. Our review revealed (a) two studies on endothelial dysfunction, out of which one found an inverse relation between plasma trimethylamine N-oxide levels and FMD and the other did not substantiate a statistically significant correlation with RHI. (b) Twelve studies on atheromatosis, assessed as intimal-medial thickness (IMT), coronary artery calcium (CAC) and arterial plaque, of which, seven studies showed statistically significant associations (negative or positive depending on the microorganism or microbiota metabolite) with IMT, one study revealed significant associations with coronary artery calcium, while one showed absence of correlation and four studies reported statistically significant correlations with arterial plaque. (c) Three studies on arterial stiffness (pulse wave velocity - PWV) with two of them concluding in statistically significant association while the third study did not. Some articles investigated multiple of the correlations described and therefore, belonged to more than one section. Conclusion: Evidence of both positive and inverse associations of gut microbiota composition and their metabolites with different types of SVD has been found. However the small number and heterogeneity of available studies cannot allow to confirm or disprove the hypothesis. © 2022 LA - English DB - MTMT ER - TY - JOUR AU - Hidi, László AU - Kovács, Gergely Imre AU - Szabó, Dóra AU - Makra, Nóra AU - Pénzes, Kinga AU - Juhász, János AU - Sótonyi, Péter AU - Ostorházi, Eszter TI - Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors JF - FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY J2 - FRONT CELL INFECT MI VL - 12 PY - 2022 PG - 10 SN - 2235-2988 DO - 10.3389/fcimb.2022.1056319 UR - https://m2.mtmt.hu/api/publication/33336219 ID - 33336219 AB - Discovery of human microbiota is fundamentally changing our perceptions of certain diseases and their treatments. However little is known about the human blood vessel microbiota, it may have important effects on vascular pathological lesions and vascular homograft failure. In our prospective survey study fourteen femoral arteries, harvested from donors in multi-organ donations, were examined using the V3-V4 region 16S rRNA sequencing method. The most abundant phyla in the human vascular microbiota were Proteobacteria , Firmicutes and Actinobacteria . At the genus level, the most abundant taxa were Staphylococcus , Corynebacterium , Pseudomonas , Bacillus , Acinetobacter and Propionibacterium . Of the bacterial taxa that have an indirect effect on the development of atherosclerosis, we found Porphyromonas gingivalis , Prevotella nigrescens and Enterobacteriaceae spp. with different abundances in our samples. Of the bacteria that are more common in the intestinal flora of healthy than of atherosclerosis patients, Roseburia and Ruminococcus occurred in the majority of samples. The human arterial wall has a unique microbiota that is significantly different in composition from that of other areas of the body. Our present study provides a basis for ensuing research that investigates the direct role of the microbiota in vascular wall abnormalities and the success of vascular allograft transplantations. LA - English DB - MTMT ER - TY - JOUR AU - Lu, Jing AU - Jin, Xiao AU - Yang, Shengjie AU - Li, Yujuan AU - Wang, Xinyue AU - Wu, Min TI - Immune mechanism of gut microbiota and its metabolites in the occurrence and development of cardiovascular diseases JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 13 PY - 2022 PG - 16 SN - 1664-302X DO - 10.3389/fmicb.2022.1034537 UR - https://m2.mtmt.hu/api/publication/33961854 ID - 33961854 N1 - Cited By :3 Export Date: 12 January 2024 Correspondence Address: Wu, M.; Guang’an Men Hospital, China; email: wumin19762000@126.com Chemicals/CAS: angiotensin II, 11128-99-7, 68521-88-0; gamma interferon, 82115-62-6; gastrin, 9002-76-0; protein kinase, 9026-43-1; retinal dehydrogenase, 37250-99-0 AB - The risk of cardiovascular disease (CVD) is associated with unusual changes in the human gut microbiota, most commonly coronary atherosclerotic heart disease, hypertension, and heart failure. Immune mechanisms maintain a dynamic balance between the gut microbiota and the host immune system. When one side changes and the balance is disrupted, different degrees of damage are inflicted on the host and a diseased state gradually develops over time. This review summarizes the immune mechanism of the gut microbiota and its metabolites in the occurrence of common CVDs, discusses the relationship between gut-heart axis dysfunction and the progression of CVD, and lists the currently effective methods of regulating the gut microbiota for the treatment of CVDs. LA - English DB - MTMT ER - TY - JOUR AU - Luo, Tiantian AU - Guo, Zhigang AU - Liu, Dan AU - Guo, Zhongzhou AU - Wu, Qiao AU - Li, Qinxian AU - Lin, Rongzhan AU - Chen, Peier AU - Ou, Caiwen AU - Chen, Minsheng TI - Deficiency of PSRC1 accelerates atherosclerosis by increasing TMAO production via manipulating gut microbiota and flavin monooxygenase 3 JF - GUT MICROBES J2 - GUT MICROBES VL - 14 PY - 2022 IS - 1 PG - 19 SN - 1949-0976 DO - 10.1080/19490976.2022.2077602 UR - https://m2.mtmt.hu/api/publication/33505979 ID - 33505979 N1 - Department of Cardiology, Laboratory of Heart Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China Guangdong Provincial Key Laboratory of Shock and Microcirculation, Guangzhou, China Department of Cardiology, State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Lab of Shock and Microcirculation, Nanfang Hospital, Southern Medical University, Guangzhou, China Department of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, China Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, China Guangdong Provincial Key Laboratory of Shock and Microcirculation, Dongguan Hospital of Southern Medical University, Southern Medical University, Guangzhou, China Cited By :9 Export Date: 12 January 2024 Correspondence Address: Chen, M.; Laboratory of Heart Center, 253 Industrial Avenue, Guangdong, China; email: gzminsheng@vip.163.com Correspondence Address: Ou, C.; Hospital of Southern Medical University, China; email: oucaiwen@smu.edu.cn Chemicals/CAS: 1 alkyl 2 acetylglycerophosphocholine esterase; ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; betaine aldehyde dehydrogenase, 9028-90-4; dimethylaniline monooxygenase, 37256-73-8; gamma interferon inducible protein 10, 97741-20-3; interleukin 13, 148157-34-0; metronidazole, 39322-38-8, 443-48-1; neomycin, 11004-65-2, 1404-04-2, 1405-10-3, 8026-22-0; phospholipase D, 9001-87-0; taurochenodeoxycholic acid, 516-35-8; trimethylamine, 75-50-3; vancomycin, 1404-90-6, 1404-93-9; methylamine, 74-89-5; mixed function oxidase, 9040-60-2; quercetin, 117-39-5; Apolipoproteins E; Flavins; Methylamines; Mixed Function Oxygenases; trimethyloxamine Tradenames: LightCycler, Hoffmann La Roche, Switzerland; PrimeScript, Takara, China Manufacturers: Hoffmann La Roche, Switzerland; Leica, Germany; Millipore; Takara, China; Thermo, United States AB - Maladaptive inflammatory and immune responses are responsible for intestinal barrier integrity and function dysregulation. Proline/serine-rich coiled-coil protein 1 (PSRC1) critically contributes to the immune system, but direct data on the gut microbiota and the microbial metabolite trimethylamine N-oxide (TMAO) are lacking. Here, we investigated the impact of PSRC1 deletion on TMAO generation and atherosclerosis. We first found that PSRC1 deletion in apoE(-/-) mice accelerated atherosclerotic plaque formation, and then the gut microbiota and metabolites were detected using metagenomics and untargeted metabolomics. Our results showed that PSRC1 deficiency enriched trimethylamine (TMA)-producing bacteria and functional potential for TMA synthesis and accordingly enhanced plasma betaine and TMAO production. Furthermore, PSRC1 deficiency resulted in a proinflammatory colonic phenotype that was significantly associated with the dysregulated bacteria. Unexpectedly, hepatic RNA-seq indicated upregulated flavin monooxygenase 3 (FMO3) expression following PSRC1 knockout. Mechanistically, PSRC1 overexpression inhibited FMO3 expression in vitro, while an ER alpha inhibitor rescued the downregulation. Consistently, PSRC1-knockout mice exhibited higher plasma TMAO levels with a choline-supplemented diet, which was gut microbiota dependent, as evidenced by antibiotic treatment. To investigate the role of dysbiosis induced by PSRC1 deletion in atherogenesis, apoE(-/-) mice were transplanted with the fecal microbiota from either apoE(-/-) or PSRC1(-/-)apoE(-/-) donor mice. Mice that received PSRC1-knockout mouse feces showed an elevation in TMAO levels, as well as plaque lipid deposition and macrophage accumulation, which were accompanied by increased plasma lipid levels and impaired hepatic cholesterol transport. Overall, we identified PSRC1 as an atherosclerosis-protective factor, at least in part, attributable to its regulation of TMAO generation via a multistep pathway. Thus, PSRC1 holds great potential for manipulating the gut microbiome and alleviating atherosclerosis. LA - English DB - MTMT ER - TY - JOUR AU - Nakayama, Ken AU - Furuyama, Tadashi AU - Matsubara, Yutaka AU - Morisaki, Koichi AU - Onohara, Toshihiro AU - Ikeda, Tetsuo AU - Yoshizumi, Tomoharu TI - Gut dysbiosis and bacterial translocation in the aneurysmal wall and blood in patients with abdominal aortic aneurysm JF - PLOS ONE J2 - PLOS ONE VL - 17 PY - 2022 IS - 12 PG - 13 SN - 1932-6203 DO - 10.1371/journal.pone.0278995 UR - https://m2.mtmt.hu/api/publication/33961853 ID - 33961853 N1 - Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Department of Vascular Surgery, National Hospital Organization Kyushu Medical Center, Fukuoka, Japan Department of Surgery and Endoscope Center, Oral Medicine Research Center, Fukuoka Dental College, Fukuoka, Japan Export Date: 12 January 2024 CODEN: POLNC Correspondence Address: Furuyama, T.; Department of Surgery and Science, Japan; email: cdq43210@par.odn.ne.jp AB - Inflammation plays a part in the development of abdominal aortic aneurysm (AAA), and the gut microbiota affects host inflammation by bacterial translocation. The relationship between abdominal aortic aneurysm and the gut microbiota remains unknown. This study aimed to detect bacterial translocation in the aneurysmal wall and blood of patients with abdominal aortic aneurysm, and to investigate the effect of the gut microbiota on abdominal aortic aneurysm. We investigated 30 patients with abdominal aortic aneurysm from 2017 to 2019. We analysed the aneurysmal wall and blood using highly sensitive reverse transcription-quantitative polymerase chain reaction, and the gut microbiota was investigated using next-generation sequencing. In the 30 patients, bacteria were detected by reverse transcription- quantitative polymerase chain reaction in 19 blood samples (detection rate, 63%) and in 11 aneurysmal wall samples (detection rate, 37%). In the gut microbiota analysis, the Firmicutes/Bacteroidetes ratio was increased. The neutrophil-lymphocyte ratio was higher (2.94 +/- 1.77 vs 1.96 +/- 0.61, P < 0.05) and the lymphocyte-monocyte ratio was lower (4.02 +/- 1.25 vs 5.86 +/- 1.38, P < 0.01) in the bacterial carrier group than in the bacterial non-carrier group in blood samples. The volume of intraluminal thrombus was significantly higher in the bacterial carrier group than in the bacterial non-carrier group in aneurysmal wall samples (64.0% vs 34.7%, P < 0.05). We confirmed gut dysbiosis and bacterial translocation to the blood and aneurysmal wall in patients with abdominal aortic aneurysm. There appears to be a relationship between the gut microbiota and abdominal aortic aneurysm. LA - English DB - MTMT ER - TY - JOUR AU - Syromyatnikov, Mikhail AU - Nesterova, Ekaterina AU - Gladkikh, Maria AU - Smirnova, Yuliya AU - Gryaznova, Mariya AU - Popov, Vasily TI - Characteristics of the Gut Bacterial Composition in People of Different Nationalities and Religions JF - MICROORGANISMS J2 - MICROORGANISMS VL - 10 PY - 2022 IS - 9 PG - 19 SN - 2076-2607 DO - 10.3390/microorganisms10091866 UR - https://m2.mtmt.hu/api/publication/33505978 ID - 33505978 N1 - Funding Agency and Grant Number: Ministry of Science and Higher Education of the Russian Federation [FZGW-2020-0001, 075001X39782002] Funding text: This work was supported by Ministry of Science and Higher Education of the Russian Federation within the framework of the national project "Science" (project FZGW-2020-0001, unique number of the register of State tasks 075001X39782002). AB - High-throughput sequencing has made it possible to extensively study the human gut microbiota. The links between the human gut microbiome and ethnicity, religion, and race remain rather poorly understood. In this review, data on the relationship between gut microbiota composition and the nationality of people and their religion were generalized. The unique gut microbiome of a healthy European (including Slavic nationality) is characterized by the dominance of the phyla Firmicutes, Bacteroidota, Actinobacteria, Proteobacteria, Fusobacteria, and Verrucomicrobia. Among the African population, the typical members of the microbiota are Bacteroides and Prevotella. The gut microbiome of Asians is very diverse and rich in members of the genera Prevotella, Bacteroides Lactobacillus, Faecalibacterium, Ruminococcus, Subdoligranulum, Coprococcus, Collinsella, Megasphaera, Bifidobacterium, and Phascolarctobacterium. Among Buddhists and Muslims, the Prevotella enterotype is characteristic of the gut microbiome, while other representatives of religions, including Christians, have the Bacteroides enterotype. Most likely, the gut microbiota of people of different nationalities and religions are influenced by food preferences. The review also considers the influences of pathologies such as obesity, Crohn's disease, cancer, diabetes, etc., on the bacterial composition of the guts of people of different nationalities. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Helga AU - Piroska, Márton AU - Hernyes, Anita AU - Zöldi, Luca AU - Juhász, János AU - Ligeti, Balázs AU - Makra, Nóra AU - Szabó, Dóra AU - Bikov, András AU - Kunos, Laszlo AU - Tárnoki, Ádám Domonkos AU - Tárnoki, Dávid László TI - The Relationship between Atherosclerosis and Gut Microbiome in Patients with Obstructive Sleep Apnoea JF - APPLIED SCIENCES-BASEL J2 - APPL SCI-BASEL VL - 12 PY - 2022 IS - 22 PG - 18 SN - 2076-3417 DO - 10.3390/app122211484 UR - https://m2.mtmt.hu/api/publication/33291618 ID - 33291618 N1 - Medical Imaging Centre, Semmelweis University, Budapest, 1082, Hungary Central Radiological Diagnostic Department, Medical Centre Hungarian Defence Forces, Budapest, 1134, Hungary Institute of Medical Microbiology, Semmelweis University, Budapest, 1085, Hungary Faculty of Information Technology and Bionics, Pazmany Peter Catholic University, Budapest, 1085, Hungary North West Lung Centre, Wythenshawe Hospital, Manchester University Foundation Trust, Manchester, M23 9LT, United Kingdom Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, The University of Manchester, Manchester, M13 9NT, United Kingdom Institute of Pulmonology, Torokbalint, 2045, Hungary Cited By :1 Export Date: 20 July 2023 Correspondence Address: Tarnoki, D.L.; Medical Imaging Centre, Hungary; email: tarnoki4@gmail.com Funding details: Manchester Biomedical Research Centre, BRC Funding details: Magyar Tudományos Akadémia, MTA, ÚNKP-20-5 Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA Funding details: Magyarország Kormánya, OTKA 138055 Funding details: Innovációs és Technológiai Minisztérium Funding details: Magyar Tüdőgyógyász Társaság, MTT Funding text 1: This research was funded by the Semmelweis Science and Innovation Fund—Research and Development Application; Hungarian Respiratory Society—Scientific Research Application; Dean’s Fund—Research Application between Theoretical and Clinical Institutes; Bólyai scholarship of the Hungarian Academy of Sciences; ÚNKP-20-5 and ÚNKP-21-5 New National Excellence Program of the Ministry for Innovation and Technology, from the source of the National Research, Development, and Innovation Fund. The bioinformatics analysis was supported by the Hungarian Government grant OTKA 138055 (Large scale surveying of bacteriophages in the human microbiome with pangenomic and machine learning approaches). Funding text 2: The Samsung ultrasound equipment was provided by Sonarmed Ltd., a Samsung Medison representative. Colleagues of the Medical Imaging Centre and Institute of Medical Microbiology, Semmelweis University, provided administrative and technical support. The authors are grateful to Electro-oxygen for providing equipment for this study and Monika Banlaky for her support with the sleep tests as well as Peter Fussy and Szonja Galyasz for their help during the microbiome measurements, and Bianka Forgo, Marcell Szily and Daniel Tamas Kovacs for their help in the OSA study. Andras Bikov is supported by the NIHR Manchester BRC. AB - Background: Obstructive sleep apnoea (OSA) and gut dysbiosis are known risk factors for atherosclerosis. However, only very few studies have been focused on the relationship between OSA, atherosclerosis, and the intestinal microbiome, all in animal models. Methods: Twenty-two patients with OSA, 16 with and 6 without carotid atherosclerosis were involved in the study. After a diagnostic sleep examination, the intima media thickness (IMT) was measured and plaques were found using carotid ultrasound. Blood was also drawn for metabolic profile, and a stool sample was provided for 16S ribosomal RNA microbiome investigation. Results: An increased maximal common carotid artery (CCA) IMT was significantly associated with decreased phylum-level diversity. The level of Peptostreptococcaceae was significantly lower in atherosclerotic subjects. Some other candidate microbes appeared in the two groups at the genus level as well: Bilophila, Romboutsia, Slackia, and Veillonella in the non-atherosclerotic group; and Escherichia-Shigella, Prevotella, and Ruminococcaceae in the atherosclerotic group. Conclusions: This is the first pilot research to analyze the association between the gut microbiome and atherosclerosis in adult patients with OSA with and without carotid atherosclerosis. Dysbiosis and individual bacteria may contribute to the development of carotid atherosclerosis in patients with OSA. Further investigations are necessary to reveal a more precise background in a larger sample. LA - English DB - MTMT ER - TY - JOUR AU - Wen, Lingmiao AU - Xiong, Wei AU - Wei, Guihua AU - Zhang, Liudai AU - Liu, Yanjun AU - Zhang, Tinglan AU - Altamirano, Alvin AU - Yin, Qiaozhi AU - Zhang, Tiane AU - Yan, Zhiyong TI - Differential Response of Ileal and Colonic Microbiota in Rats with High-Fat Diet-Induced Atherosclerosis JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 19 PG - 17 SN - 1661-6596 DO - 10.3390/ijms231911154 UR - https://m2.mtmt.hu/api/publication/33505977 ID - 33505977 N1 - School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, AZ 86011, United States School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China Cited By :2 Export Date: 12 January 2024 Correspondence Address: Yan, Z.; School of Life Science and Engineering, China; email: yzhiy@swjtu.edu.cn Correspondence Address: Zhang, T.; School of Basic Medicine, China; email: zhte2003@cdutcm.edu.cn Chemicals/CAS: lipid, 66455-18-3 AB - Growing evidence suggests that gut microbiota are associated with atherosclerosis (AS). However, the functional heterogeneity of each gut segment gives rise to regional differences in gut microbiota. We established a rat model of AS by feeding the rats a high-fat diet for a long period. The pathological and microbiota changes in the ileum and colon of the rats were examined, and correlations between AS and microbiota were analyzed. The aortic mesothelium of the experimental rats was damaged. The intima showed evident calcium salt deposition, indicating that the AS rat model was successfully developed. We noted varying degrees of pathological damage in the ileum and colon of the experimental rats. The 16S rDNA high-throughput sequencing showed significant differences in alpha-diversity, beta-diversity, and microbiota comparisons in the ileum and colon. Furthermore, the ileum and colon of AS rats showed varying degrees of intestinal microbiota disturbance. This article contributes to the study of the relationship between the microbiota in different regions of the gut and AS, and provides new approaches in gut microbiota intervention for the treatment of AS. LA - English DB - MTMT ER - TY - JOUR AU - Shen, Xinyi AU - Li, Lihua AU - Sun, Zhen AU - Zang, Guangyao AU - Zhang, Lili AU - Shao, Chen AU - Wang, Zhongqun TI - Gut Microbiota and Atherosclerosis-Focusing on the Plaque Stability JF - FRONTIERS IN CARDIOVASCULAR MEDICINE J2 - FRONT CARDIOVASC MED VL - 8 PY - 2021 PG - 15 SN - 2297-055X DO - 10.3389/fcvm.2021.668532 UR - https://m2.mtmt.hu/api/publication/32298569 ID - 32298569 N1 - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, China Department of Pathology, Affiliated Hospital of Jiangsu University, Zhenjiang, China Cited By :30 Export Date: 12 January 2024 Correspondence Address: Wang, Z.; Department of Cardiology, China; email: wangtsmc@126.com AB - Cardiovascular diseases (CVDs) are major causes of mortality and morbidity in the modern society. The rupture of atherosclerotic plaque can induce thrombus formation, which is the main cause of acute cardiovascular events. Recently, many studies have demonstrated that there are some relationships between microbiota and atherosclerosis. In this review, we will focus on the effect of the microbiota and the microbe-derived metabolites, including trimethylamine-N-oxide (TMAO), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS), on the stability of atherosclerotic plaque. Finally, we will conclude with some therapies based on the microbiota and its metabolites. LA - English DB - MTMT ER -