TY - JOUR AU - Das, Kaushik AU - Rao, L. Vijaya Mohan TI - The Role of microRNAs in Inflammation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 24 SN - 1661-6596 DO - 10.3390/ijms232415479 UR - https://m2.mtmt.hu/api/publication/33298784 ID - 33298784 AB - Inflammation is a biological response of the immune system to various insults, such as pathogens, toxic compounds, damaged cells, and radiation. The complex network of pro- and anti-inflammatory factors and their direction towards inflammation often leads to the development and progression of various inflammation-associated diseases. The role of small non-coding RNAs (small ncRNAs) in inflammation has gained much attention in the past two decades for their regulation of inflammatory gene expression at multiple levels and their potential to serve as biomarkers and therapeutic targets in various diseases. One group of small ncRNAs, microRNAs (miRNAs), has become a key regulator in various inflammatory disease conditions. Their fine-tuning of target gene regulation often turns out to be an important factor in controlling aberrant inflammatory reactions in the system. This review summarizes the biogenesis of miRNA and the mechanisms of miRNA-mediated gene regulation. The review also briefly discusses various pro- and anti-inflammatory miRNAs, their targets and functions, and provides a detailed discussion on the role of miR-10a in inflammation. LA - English DB - MTMT ER - TY - JOUR AU - Khodzhaeva, Z.S. AU - Oshkhunova, M.S. AU - Muminova, K.T. AU - Gorina, K.A. AU - Kholin, A.M. TI - PREDICTION AND EARLY DIAGNOSIS OF PREECLAMPSIA: SCIENTIFIC PERSPECTIVES AND CLINICAL OPPORTUNITIES JF - AKUSHERSTVO I GINEKOLOGIIA - MOSKVA J2 - AKUSH GINEKOL VL - 2022 PY - 2022 IS - 12 SP - 57 EP - 65 PG - 9 SN - 0300-9092 DO - 10.18565/aig.2022.218 UR - https://m2.mtmt.hu/api/publication/33623570 ID - 33623570 N1 - Obstetrics Institute, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, Akademika Oparina str., 4, Moscow, 117997, Russian Federation High Risk Pregnancy Department, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, Akademika Oparina str., 4, Moscow, 117997, Russian Federation Telemedicine Section, Department of Regional Cooperation and Integration, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, Akademika Oparina str., 4, Moscow, 117997, Russian Federation Export Date: 6 February 2023 CODEN: AKGIA LA - Russian DB - MTMT ER - TY - JOUR AU - Li, Zhan AU - Ru, Xiaoli AU - Wang, Shuzhen AU - Cao, Guangming TI - miR‐24‐3p regulation of retinol binding protein 4 in trophoblast biofunction and preeclampsia JF - MOLECULAR REPRODUCTION AND DEVELOPMENT J2 - MOL REPROD DEV VL - e PY - 2022 IS - 1 SP - 1 SN - 1040-452X DO - 10.1002/mrd.23633 UR - https://m2.mtmt.hu/api/publication/32949181 ID - 32949181 LA - English DB - MTMT ER - TY - JOUR AU - Shu, Chang AU - Xu, Peng AU - Han, Jun AU - Han, Shumei AU - He, Jin TI - Upregulation of circRNA hsa_circ_0008726 in Pre-eclampsia Inhibits Trophoblast Migration, Invasion, and EMT by Regulating miR-345-3p/RYBP Axis JF - REPRODUCTIVE SCIENCES J2 - REPROD SCI VL - 29 PY - 2022 IS - 10 SP - 2829 EP - 2841 PG - 13 SN - 1933-7191 DO - 10.1007/s43032-021-00804-y UR - https://m2.mtmt.hu/api/publication/32517799 ID - 32517799 LA - English DB - MTMT ER - TY - JOUR AU - Wang, Yinfen AU - Chen, Aner TI - Mast cell‐derived exosomal miR ‐181a‐5p modulated trophoblast cell viability, migration, and invasion via YY1 / MMP ‐9 axis JF - JOURNAL OF CLINICAL LABORATORY ANALYSIS J2 - J CLIN LAB ANAL VL - e24549 PY - 2022 SN - 0887-8013 DO - 10.1002/jcla.24549 UR - https://m2.mtmt.hu/api/publication/32888348 ID - 32888348 LA - English DB - MTMT ER - TY - JOUR AU - Ashraf, Usman M. AU - Hall, Dalton L. AU - Rawls, Adam Z. AU - Alexander, Barbara T. TI - Epigenetic processes during preeclampsia and effects on fetal development and chronic health JF - CLINICAL SCIENCE J2 - CLIN SCI VL - 135 PY - 2021 IS - 19 SP - 2307 EP - 2327 PG - 21 SN - 0143-5221 DO - 10.1042/CS20190070 UR - https://m2.mtmt.hu/api/publication/32480054 ID - 32480054 LA - English DB - MTMT ER - TY - JOUR AU - Han, L. AU - Luo, Q.-Q. AU - Peng, M.-G. AU - Zhang, Y. AU - Zhu, X.-H. TI - miR-483 is downregulated in pre-eclampsia via targeting insulin-like growth factor 1 (IGF1) and regulates the PI3K/Akt/mTOR pathway of endothelial progenitor cells JF - JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH J2 - J OBSTET GYNAECOL RES VL - 47 PY - 2021 IS - 1 SP - 63 EP - 72 PG - 10 SN - 1341-8076 DO - 10.1111/jog.14412 UR - https://m2.mtmt.hu/api/publication/31625150 ID - 31625150 LA - English DB - MTMT ER - TY - JOUR AU - Pankiewicz, K. AU - Fijałkowska, A. AU - Issat, T. AU - Maciejewski, T.M. TI - Insight into the key points of preeclampsia pathophysiology: Uterine artery remodeling and the role of micrornas JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 6 PG - 14 SN - 1661-6596 DO - 10.3390/ijms22063132 UR - https://m2.mtmt.hu/api/publication/31932623 ID - 31932623 N1 - Department of Obstetrics and Gynecology, Institute of Mother and Child in Warsaw, Kasprzaka 17a, Warsaw, 01-211, Poland Department of Cardiology, Institute of Mother and Child in Warsaw, Kasprzaka 17a, Warsaw, 01-211, Poland Export Date: 26 March 2021 Correspondence Address: Pankiewicz, K.; Department of Obstetrics and Gynecology, Kasprzaka 17a, Poland; email: katarzynahak@wp.pl Department of Obstetrics and Gynecology, Institute of Mother and Child in Warsaw, Kasprzaka 17a, Warsaw, 01-211, Poland Department of Cardiology, Institute of Mother and Child in Warsaw, Kasprzaka 17a, Warsaw, 01-211, Poland Export Date: 30 March 2021 Correspondence Address: Pankiewicz, K.; Department of Obstetrics and Gynecology, Kasprzaka 17a, Poland; email: katarzynahak@wp.pl Department of Obstetrics and Gynecology, Institute of Mother and Child in Warsaw, Kasprzaka 17a, Warsaw, 01-211, Poland Department of Cardiology, Institute of Mother and Child in Warsaw, Kasprzaka 17a, Warsaw, 01-211, Poland Export Date: 16 May 2021 Correspondence Address: Pankiewicz, K.; Department of Obstetrics and Gynecology, Kasprzaka 17a, Poland; email: katarzynahak@wp.pl AB - Preeclampsia affects about 3–8% of all pregnancies. It represents a complex and multifaceted syndrome with at least several potential pathways leading to the development of disease. The main dogma in preeclampsia is the two-stage model of disease. Stage 1 (placental stage) takes place in early pregnancy and is thought to be impaired placentation due to inadequate trophoblastic invasion of the maternal spiral arteries that leads to reduced placental perfusion and release of numerous biological factors causing endothelial damage and development of acute maternal syndrome with systemic multiorgan failure (stage 2—the onset of maternal clinical symptoms, maternal stage). Recently, in the light of the vast body of evidence, two-stage model of preeclampsia has been updated with a few novel pathways leading to clinical manifestation in the second part of pregnancy. This paper reviews current state of knowledge about pathophysiology of preeclampsia and places particular focus on the recent advances in understanding of uterine artery remodeling alterations, as well as the role of microRNAs in preeclampsia. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - CHAP AU - Safran, Marilyn AU - Rosen, Naomi AU - Twik, Michal AU - BarShir, Ruth AU - Stein, Tsippi Iny AU - Dahary, Dvir AU - Fishilevich, Simon AU - Lancet, Doron ED - Kasukawa, Takeya ED - Abugessaisa, Imad TI - The GeneCards Suite T2 - Practical Guide to Life Science Databases PB - Springer-Verlag Singapore SN - 9789811658112 PY - 2021 SP - 27 EP - 56 PG - 30 DO - 10.1007/978-981-16-5812-9_2 UR - https://m2.mtmt.hu/api/publication/32581494 ID - 32581494 LA - English DB - MTMT ER - TY - JOUR AU - Shan, Li AU - Hou, Xiaofei TI - Circular RNA hsa_circ_0026552 inhibits the proliferation, migration and invasion of trophoblast cells via the miR‑331‑3p/TGF‑βR1 axis in pre‑eclampsia JF - MOLECULAR MEDICINE REPORTS J2 - MOL MED REP VL - 24 PY - 2021 IS - 5 SN - 1791-2997 DO - 10.3892/mmr.2021.12438 UR - https://m2.mtmt.hu/api/publication/32269801 ID - 32269801 LA - English DB - MTMT ER - TY - JOUR AU - Benny, P.A. AU - Alakwaa, F.M. AU - Schlueter, R.J. AU - Lassiter, C.B. AU - Garmire, L.X. TI - A review of omics approaches to study preeclampsia JF - PLACENTA J2 - PLACENTA VL - 92 PY - 2020 SP - 17 EP - 27 PG - 11 SN - 0143-4004 DO - 10.1016/j.placenta.2020.01.008 UR - https://m2.mtmt.hu/api/publication/31188708 ID - 31188708 N1 - University of Hawaii Cancer Center, Epidemiology, 701 Ilalo Street, Honolulu, HI 96813, United States Department of Obstetrics and Gynaecology, University of Hawaii, 1319 Punahou Street, Honolulu, HI 96826, United States Department of Computational Medicine and Bioinformatics, North Campus Research Complex, University of Michigan, 1600 Huron Parkway, Ann Arbor, MI 48105, United States Export Date: 20 February 2020 CODEN: PLACD Correspondence Address: Garmire, L.X.; Department of Computational Medicine and Bioinformatics, North Campus Research Complex, University of Michigan, 1600 Huron Parkway, United States; email: LGarmire@med.umich.edu Chemicals/CAS: inhibin, 57285-09-3, 69494-61-7 Funding details: U.S. National Library of Medicine, NLM, R01 HD084633 Funding details: P20 COBRE GM103457 Funding details: R01 LM012373, LM 012907 Funding details: Eunice Kennedy Shriver National Institute of Child Health and Human Development, NICHD Funding details: National Institute of Child Health and Human Development, NICHD Funding details: Department of Obstetrics and Gynecology, University of Wisconsin-Madison, OBGYN Funding details: U.S. National Library of Medicine, NLM Funding text 1: Dr. Lana X Garmire's research is supported by grants K01ES025434 awarded by National Insitute of Enviromental Health Sciences through funds provided by the trans-National Insitute of Health (NIH) Big Data to Knowledge (BD2K) initiative ( http://datascience.nih.gov/bd2k ), P20 COBRE GM103457 awarded by National Insitute of General Medical Sciences (NIGMS) , R01 LM012373 and LM 012907 awarded by National Library of Medicine ( NLM) , and R01 HD084633 awarded by National Institute of Child Health and Human Development ( NICHD) to LX Garmire. Funding was also provided in part by the Department of Obstetrics and Gynecology, University of Hawaii . The authors thank all members of the Garmire Group for their helpful discussions. University of Hawaii Cancer Center, Epidemiology, 701 Ilalo Street, Honolulu, HI 96813, United States Department of Obstetrics and Gynaecology, University of Hawaii, 1319 Punahou Street, Honolulu, HI 96826, United States Department of Computational Medicine and Bioinformatics, North Campus Research Complex, University of Michigan, 1600 Huron Parkway, Ann Arbor, MI 48105, United States Cited By :1 Export Date: 18 August 2020 CODEN: PLACD Correspondence Address: Garmire, L.X.; Department of Computational Medicine and Bioinformatics, North Campus Research Complex, University of Michigan, 1600 Huron Parkway, United States; email: LGarmire@med.umich.edu Chemicals/CAS: inhibin, 57285-09-3, 69494-61-7 Funding details: U.S. National Library of Medicine, NLM, R01 HD084633 Funding details: P20 COBRE GM103457 Funding details: R01 LM012373, LM 012907 Funding details: Eunice Kennedy Shriver National Institute of Child Health and Human Development, NICHD Funding details: National Institute of Child Health and Human Development, NICHD Funding details: Department of Obstetrics and Gynecology, University of Wisconsin-Madison, OBGYN Funding details: U.S. National Library of Medicine, NLM Funding text 1: Dr. Lana X Garmire's research is supported by grants K01ES025434 awarded by National Insitute of Enviromental Health Sciences through funds provided by the trans-National Insitute of Health (NIH) Big Data to Knowledge (BD2K) initiative ( http://datascience.nih.gov/bd2k ), P20 COBRE GM103457 awarded by National Insitute of General Medical Sciences (NIGMS) , R01 LM012373 and LM 012907 awarded by National Library of Medicine ( NLM) , and R01 HD084633 awarded by National Institute of Child Health and Human Development ( NICHD) to LX Garmire. Funding was also provided in part by the Department of Obstetrics and Gynecology, University of Hawaii . The authors thank all members of the Garmire Group for their helpful discussions. University of Hawaii Cancer Center, Epidemiology, 701 Ilalo Street, Honolulu, HI 96813, United States Department of Obstetrics and Gynaecology, University of Hawaii, 1319 Punahou Street, Honolulu, HI 96826, United States Department of Computational Medicine and Bioinformatics, North Campus Research Complex, University of Michigan, 1600 Huron Parkway, Ann Arbor, MI 48105, United States Cited By :4 Export Date: 14 May 2021 CODEN: PLACD Correspondence Address: Garmire, L.X.; Department of Computational Medicine and Bioinformatics, 1600 Huron Parkway, United States; email: LGarmire@med.umich.edu LA - English DB - MTMT ER - TY - JOUR AU - Liu, L. TI - Values of serum miR-873 and miR-323-3p levels in diagnosis of ectopic pregnancy JF - ZHONGGUO YISHI ZAZHI / JOURNAL OF CHINESE PHYSICIAN J2 - J CHIN PHYS VL - 22 PY - 2020 IS - 5 SP - 745 EP - 748 PG - 4 SN - 1008-1372 DO - 10.3760/cma.j.cn431274-20190125-00079 UR - https://m2.mtmt.hu/api/publication/31625149 ID - 31625149 N1 - Export Date: 11 October 2020 LA - Chinese DB - MTMT ER - TY - JOUR AU - Ma, H. -Y. AU - Cu, W. AU - Sun, Y. -H. AU - Chen, X. TI - MiRNA-203a-3p inhibits inflammatory response in preeclampsia through regulating IL24 JF - EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES J2 - EUR REV MED PHARMACOL SCI VL - 24 PY - 2020 IS - 10 SP - 5223 EP - 5230 PG - 8 SN - 1128-3602 UR - https://m2.mtmt.hu/api/publication/31344479 ID - 31344479 LA - English DB - MTMT ER - TY - JOUR AU - Ou, Yuhua AU - Zhu, Liqiong AU - Wei, Xiangcai AU - Bai, Shiyu AU - Chen, Manqi AU - Chen, Hui AU - Zhang, Jianping TI - Circular RNA circ_0111277 attenuates human trophoblast cell invasion and migration by regulating miR-494/HTRA1/Notch-1 signal pathway in pre-eclampsia JF - CELL DEATH AND DISEASE J2 - CELL DEATH DIS VL - 11 PY - 2020 IS - 6 SN - 2041-4889 DO - 10.1038/s41419-020-2679-6 UR - https://m2.mtmt.hu/api/publication/31361659 ID - 31361659 LA - English DB - MTMT ER - TY - JOUR AU - Setiawan, William Alexander AU - Pranamartha, A.A. Gde Marvy Khrisna TI - The role of circular Ribonucleic Acid in preeclampsia: a literature review JF - Indonesian Journal of Perinatology J2 - InaJPerinatol VL - 1 PY - 2020 IS - 1 SP - 13 EP - 17 PG - 5 SN - 2775-0744 DO - 10.51559/inajperinatol.v1i1.4 UR - https://m2.mtmt.hu/api/publication/32513978 ID - 32513978 LA - English DB - MTMT ER - TY - JOUR AU - Wang, Xiaoning AU - Peng, Shiyuan AU - Cui, Kun AU - Hou, Fangjuan AU - Ding, Jie AU - li, Ali AU - Wang, Mingxia AU - Geng, Li TI - MicroRNA-576-5p enhances the invasion ability of trophoblast cells in preeclampsia by targeting TFAP2A JF - MOLECULAR GENETICS AND GENOMIC MEDICINE J2 - MOL GENET GENOM MED VL - 8 PY - 2020 IS - 1 SN - 2324-9269 DO - 10.1002/mgg3.1025 UR - https://m2.mtmt.hu/api/publication/31002880 ID - 31002880 AB - Background Preeclampsia (PE) is a common pregnancy-related syndrome characterized by hypertension and proteinuria, and a major cause of maternal mortality. Therefore, there is an urgent need to identify early biomarkers of PE. The aim of the present study was to identify the functions of miR-576-5p in PE. Methods Effects of miR-576-5p and transcription factor AP-2 alpha (TFAP2A) on invasion of human trophoblast HTR8/SVneo cells were investigated. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting were used to assess the expression of miR-576-5p, TFAP2A, E-cad, and Vimentin in PE tissues and cells. Additionally, immunofluorescence was used to detect the expression of TFAP2A in PE trophoblastic tissue. Subsequently, constructed miR-576-5p mimics, miR-576-5p inhibitor, and siRNA-TFAP2A plasmids were transfected into HTR8/SVneo cells for further experiments, including a CCK-8 assay for cell proliferation, Transwell assay for cell invasion and the luciferase reporter gene system was employed for target verification. Results A lower expression of miR-576-5p and a higher expression of TFAP2A were identified in PE rats. E-cadherin was highly expressed while Vimentin was downregulated. Further statistical analysis indicated that cell proliferation of HTR8/SVneo cells decreased in the miR-576-5p inhibitor group and increased in the miR-576-5p mimics and siRNA-TFAP2A groups. miR-576-5p inhibitor suppressed cell invasion, and miR-576-5p mimics and siRNA-TFAP2A improved cell invasion. The analysis of luciferase reporter demonstrated a decreased luciferase activity in miR-576-5p mimics group compared with control group, which indicates that TFAP2A may be a target of miR-576-5p. Interference of TFAP2A could downregulate E-cadherin and upregulate Vimentin expression. Conclusion Overexpression of miR-576-5p and knockdown of TFAP2A may elevate cell proliferation and invasion of human trophoblast cells in vitro. Therefore, miR-576-5p may be used as a notable biomarker for the diagnosis, prevention, and treatment of PE. miR-576-5p targeting TFAP2A deserve further investigation in order to explore their potential role in PE. LA - English DB - MTMT ER - TY - JOUR AU - Zhao, Yan-Hua AU - Liu, Yue-Lan AU - Fei, Kui-Lin AU - Li, Ping TI - Long non-coding RNA HOTAIR modulates the progression of preeclampsia through inhibiting miR-106 in an EZH2-dependent manner JF - LIFE SCIENCES J2 - LIFE SCI VL - 253 PY - 2020 SN - 0024-3205 DO - 10.1016/j.lfs.2020.117668 UR - https://m2.mtmt.hu/api/publication/31344486 ID - 31344486 N1 - Export Date: 29 June 2020 CODEN: LIFSA LA - English DB - MTMT ER - TY - JOUR AU - Anton, Lauren AU - DeVine, Ann AU - Polyak, Erzsebet AU - Olarerin-George, Anthony AU - Brown, Amy G. AU - Falk, Marni J. AU - Elovitz, Michal A. TI - HIF-1 alpha Stabilization Increases miR-210 Eliciting First Trimester Extravillous Trophoblast Mitochondrial Dysfunction JF - FRONTIERS IN PHYSIOLOGY J2 - FRONT PHYSIOL VL - 10 PY - 2019 PG - 12 SN - 1664-042X DO - 10.3389/fphys.2019.00699 UR - https://m2.mtmt.hu/api/publication/31002885 ID - 31002885 AB - Preeclampsia is associated with first trimester placental dysfunction. miR-210, a small non-coding RNA, is increased in the preeclamptic placenta. The effects of elevated miR-210 on placental function remain unclear. The objectives of this study were to identify targets of miR-210 in first trimester primary extravillous trophoblasts (EVTs) and to investigate functional pathways altered by elevated placental miR-210 during early pregnancy. EVTs isolated from first trimester placentas were exposed to cobalt chloride (CoCl2), a HIF-1 alpha stabilizer and hypoxia mimetic, and miR-210 expression by qPCR, HIF1 alpha protein levels by western blot and cell invasion were assessed. A custom TruSeq RNA array, including all known/predicted miR-210 targets, was run using miR-210 and miR-negative control transfected EVTs. Mitochondrial function was assessed by high resolution respirometry in transfected EVTs. EVTs exposed to CoCl2 showed a dose and time-dependent increase in miR-210 and HIF1 alpha and reductions in cell invasion. The TruSeq array identified 49 altered genes in miR-210 transfected EVTs with 27 genes repressed and 22 enhanced. Three of the top six significantly repressed genes, NDUFA4, SDHD, and ISCU, are associated with mitochondrial function. miR-210 transfected EVTs had decreased maximal, complex II and complex I+II mitochondrial respiration. This study suggests that miR-210 alters first trimester trophoblast function. miR-210 overexpression alters EVT mitochondrial function in early pregnancy. Mitochondrial dysfunction may lead to increased reactive oxygen species, trophoblast cell damage and likely contributes to the pathogenesis of preeclampsia. LA - English DB - MTMT ER - TY - JOUR AU - Apicella, Clara AU - Ruano, Camino S. M. AU - Méhats, Céline AU - Miralles, Francisco AU - Vaiman, Daniel TI - The Role of Epigenetics in Placental Development and the Etiology of Preeclampsia JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 20 PY - 2019 IS - 11 SN - 1661-6596 DO - 10.3390/ijms20112837 UR - https://m2.mtmt.hu/api/publication/30714315 ID - 30714315 N1 - Export Date: 24 April 2024 LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Fóthi, Ábel AU - Alasztics, Bálint AU - Nagy, Bálint AU - Orbán, Tamás I. AU - Rigó, János TI - Circulating exosomal and Argonaute-bound microRNAs in preeclampsia JF - GENE J2 - GENE VL - 692 ET - 0 PY - 2019 SP - 138 EP - 144 PG - 7 SN - 0378-1119 DO - 10.1016/j.gene.2019.01.012 UR - https://m2.mtmt.hu/api/publication/30401681 ID - 30401681 AB - Introduction microRNAs (miRNAs) play important role in the regulation of placental development, and abnormal miRNA expression is associated with preeclampsia (PE). miRNAs are released from trophoblast cells to maternal blood flow, where they are highly stable, being encapsulated inside extracellular vesicles, like exosomes or bound to Argonaute proteins. In PE, placental dysfunction leads to aberrant extracellular miRNA secretion. hsa-miR-210 is a hypoxia-sensitive miRNA found to be upregulated in PE; however, it is unknown whether it is the cause or the consequence of the disease. Objective Our aim was to analyze the expression of several miRNAs, including hsa-miR-210 in placenta, exosome and Ago-bound fractions comparing normal (N) and PE pregnancies. We performed in vitro analyses of extracellular hsa-miR-210 secretion of trophoblast cell cultures (of villous and extravillous origin) under hypoxic condition. Methods PE and N placenta samples were collected from C-sections, and blood samples were drawn from each pregnant woman in the third trimester. HTR-8 and JAR cell lines were cultured in exosome-free media and treated with hypoxia-mimetic agents. Exosome and Ago-bound fractions were isolated by membrane affinity spin column method from plasma and cell media. Short RNAs were extracted from exosomes and vesicle-free fractions, and total-RNA was isolated from the placenta samples. The RNA purity and concentration were measured by spectrophotometry. Expression analysis was carried out by qPCR with specific primers to target and reference miRNAs. Results The level of hsa-miR-210 was significantly higher in PE placentas, which could cause a minor increase of exosomal and a high elevation of Ago-bound miR-210 in circulation. Hypoxia lead to intracellular hsa-miR-210 upregulation in trophoblast cell lines. In extravillous cell (HTR-8) media, only the level of exosomal hsa-miR-210 was increased but no change in Ago-bound hsa-miR-210 level was observed. In contrast, in villous cell (JAR) media, the level of exosomal hsa-miR-210 was increased and enhanced release of Ago-bound hsa-miR-210 was also observed. Conclusion Based on our data, we postulate that in PE, exosomal hsa-miR-210 is secreted actively from the trophoblast, and by intercellular communication, it may have a role in disease etiology. In addition, there is a passive release of Ago-bound hsa-miR-210 into the circulation, which may represent by-products of cell-death and is thereby a possible consequence of the disease. LA - English DB - MTMT ER - TY - JOUR AU - Jia, Ningyi AU - Li, Jian TI - Role of Circular RNAs in Preeclampsia JF - DISEASE MARKERS J2 - DIS MARKERS VL - 2019 PY - 2019 IS - 1 SP - 1 EP - 7 PG - 7 SN - 0278-0240 DO - 10.1155/2019/7237495 UR - https://m2.mtmt.hu/api/publication/30662422 ID - 30662422 LA - English DB - MTMT ER - TY - JOUR AU - Liu, Siwei AU - Xie, Xie AU - Lei, Huajiang AU - Zou, Bingyu AU - Xie, Lan TI - Identification of Key circRNAs/lncRNAs/miRNAs/mRNAs and Pathways in Preeclampsia Using Bioinformatics Analysis JF - MEDICAL SCIENCE MONITOR J2 - MED SCI MONIT VL - 25 PY - 2019 SP - 1679 EP - 1693 PG - 15 SN - 1234-1010 DO - 10.12659/MSM.912801 UR - https://m2.mtmt.hu/api/publication/30701571 ID - 30701571 LA - English DB - MTMT ER - TY - JOUR AU - Yang, X. AU - Guo, F. TI - MiR-342-3p suppresses cell migration and invasion in preeclampsia by targeting platelet-derived growth factor receptor a JF - MOLECULAR MEDICINE REPORTS J2 - MOL MED REP VL - 20 PY - 2019 IS - 2 SP - 1772 EP - 1780 PG - 9 SN - 1791-2997 DO - 10.3892/mmr.2019.10372 UR - https://m2.mtmt.hu/api/publication/30746878 ID - 30746878 N1 - Export Date: 15 December 2020 Correspondence Address: Yang, X.; Department of Obstetrics, First Hospital of China Medical University, 155 Nanjing north Street, China; email: 57470040@qq.com LA - English DB - MTMT ER - TY - JOUR AU - Zaiou, Mohamed TI - Circular RNAs in hypertension: challenges and clinical promise JF - HYPERTENSION RESEARCH J2 - HYPERTENS RES VL - 42 PY - 2019 IS - 11 SP - 1653 EP - 1663 PG - 11 SN - 0916-9636 DO - 10.1038/s41440-019-0294-7 UR - https://m2.mtmt.hu/api/publication/30727239 ID - 30727239 LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Rigó, János TI - A mikro-RNS-ek patogenetikai szerepe és expressziós mintázata praeeclampsiában JF - ORVOSI HETILAP J2 - ORV HETIL VL - 159 PY - 2018 IS - 14 SP - 547 EP - 556 PG - 10 SN - 0030-6002 DO - 10.1556/650.2018.31025 UR - https://m2.mtmt.hu/api/publication/3357249 ID - 3357249 AB - Preeclampsia is the leading cause of maternal and fetal morbidity and mortality that affects 3-8% of pregnancies worldwide. Its main symptoms include new onset of high blood pressure and proteinuria after 20 weeks of pregnancy. The cause of the disease is still debated. microRNAs are short, non-coding RNA molecules that play a pivotal part in the posttranscriptional regulation of eukaryotic genes. They are involved in fine-tuning of vital physiological processes such as cell cycle, proliferation, differentiation and cell death. In genomic studies, hundreds of microRNAs were detected in the placenta, which are supposed to regulate placental development and contribute to uncomplicated pregnancy. Several studies have reported changes in the expression of microRNAs in pregnancy. Abnormal microRNA expression may have a role in the development of preeclampsia as it affects the proliferation, migration, and invasion of the trophoblast cells, spiral artery remodeling, and angiogenesis. Some placental microRNAs (e.g., the C19MC microRNA cluster) are able to reach the maternal circulation through their release via exosomes from the trophoblast layer. These 'circulating' microRNA molecules can be applied as biomarkers for the detection of various placental disorders owing to their stability and specificity. Orv Hetil. 2018; 159(14): 547-556. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hu, Xiaopeng AU - Ao, Junping AU - Li, Xinyue AU - Zhang, Huijuan AU - Wu, Ji AU - Cheng, Weiwei TI - Competing endogenous RNA expression profiling in pre-eclampsia identifies hsa_circ_0036877 as a potential novel blood biomarker for early pre-eclampsia JF - CLINICAL EPIGENETICS J2 - CLIN EPIGENETICS VL - 10 PY - 2018 IS - 1 SN - 1868-7075 DO - 10.1186/s13148-018-0482-3 UR - https://m2.mtmt.hu/api/publication/27371799 ID - 27371799 LA - English DB - MTMT ER - TY - JOUR AU - Mavreli, D AU - Papantoniou, N AU - Kolialexi, A TI - miRNAs in pregnancy-related complications: an update JF - EXPERT REVIEW OF MOLECULAR DIAGNOSTICS J2 - EXPERT REV MOL DIAGN VL - 18 PY - 2018 IS - 7 SP - 587 EP - 589 PG - 3 SN - 1473-7159 DO - 10.1080/14737159.2018.1480939 UR - https://m2.mtmt.hu/api/publication/27546979 ID - 27546979 N1 - OA gold_or_bronze 3rd Department of Obstetrics & Gynecology, Athens University School of Medicine, Athens, Greece Department of Medical Genetics, Athens University School of Medicine, Athens, Greece Cited By :1 Export Date: 23 August 2019 CODEN: ERMDC Correspondence Address: Kolialexi, A.; Department of Medical Genetics, Aghia Sofia Children’s HospitalGreece; email: akolial@med.uoa.gr Chemicals/CAS: Biomarkers; MicroRNAs Export Date: 9 February 2020 CODEN: ERMDC Correspondence Address: Kolialexi, A.; Department of Medical Genetics, Aghia Sofia Children’s HospitalGreece; email: akolial@med.uoa.gr LA - English DB - MTMT ER - TY - JOUR AU - Rezaei, Mahnaz AU - Eskandari, Fatemeh AU - Mohammadpour-Gharehbagh, Abbas AU - Teimoori, Batool AU - Yaghmaei, Minoo AU - Mokhtari, Mojgan AU - Salimi, Saeedeh TI - The Drosha rs10719 T > C polymorphism is associated with preeclampsia susceptibility JF - CLINICAL AND EXPERIMENTAL HYPERTENSION J2 - CLIN EXP HYPERTENS VL - 40 PY - 2018 IS - 5 SP - 440 EP - 445 PG - 6 SN - 1064-1963 DO - 10.1080/10641963.2017.1392555 UR - https://m2.mtmt.hu/api/publication/27513917 ID - 27513917 LA - English DB - MTMT ER - TY - JOUR AU - Schechinger, M AU - Marks, H AU - Locke, A AU - Choudhury, M AU - Cote, G TI - Development of a miRNA surface-enhanced Raman scattering assay using benchtop and handheld Raman systems JF - JOURNAL OF BIOMEDICAL OPTICS J2 - J BIOMED OPT VL - 23 PY - 2018 IS - 1 SN - 1083-3668 DO - 10.1117/1.JBO.23.1.017002 UR - https://m2.mtmt.hu/api/publication/27231049 ID - 27231049 LA - English DB - MTMT ER - TY - JOUR AU - Bai, Y AU - Zhang, X AU - Yang, X AU - Li, F TI - Relevance of microRNA-122 to pathogenesis of preeclampsia in rats JF - INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE J2 - INT J CLIN EXP MED VL - 10 PY - 2017 IS - 7 SP - 10103 EP - 10112 PG - 10 SN - 1940-5901 UR - https://m2.mtmt.hu/api/publication/26853085 ID - 26853085 LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Alasztics, Bálint AU - Molvarec, Attila AU - Joó, József Gábor AU - Nagy, Bálint AU - Rigó, János TI - Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension JF - PREGNANCY HYPERTENSION J2 - PREGNANCY HYPERTENS VL - 10 PY - 2017 SP - 207 EP - 212 PG - 6 SN - 2210-7789 DO - 10.1016/j.preghy.2017.09.002 UR - https://m2.mtmt.hu/api/publication/3273020 ID - 3273020 AB - Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy. LA - English DB - MTMT ER - TY - JOUR AU - Orzińska, Agnieszka TI - Wybrane zagadnienia dotyczące badań nad wolnokrążącym materiałem genetycznym płodu w świetle doniesień prezentowanych na IV Środkowo-Wschodnim Europejskim Sympozjum na temat wolnokrążących kwasów nukleinowych w nieinwazyjnej diagnostyce prenatalnej JF - Journal of Transfusion Medicine VL - 10 PY - 2017 IS - 2 SP - 73 EP - 75 PG - 3 SN - 2080-1505 UR - https://m2.mtmt.hu/api/publication/31344500 ID - 31344500 LA - Polish DB - MTMT ER - TY - JOUR AU - Pan, Q AU - Niu, H AU - Cheng, L AU - Li, X AU - Zhang, Q AU - Ning, Y TI - Invasion of trophoblast cell lines is inhibited by miR-93 via MMP-2 JF - PLACENTA J2 - PLACENTA VL - 53 PY - 2017 SP - 48 EP - 53 PG - 6 SN - 0143-4004 DO - 10.1016/j.placenta.2017.03.008 UR - https://m2.mtmt.hu/api/publication/26853043 ID - 26853043 N1 - RETRACTED / VISSZAVONT AB - Preeclampsia (PE) is a serious pregnancy-related syndrome, which is characterized by gestational hypertension and proteinuria. The microRNA-93 (miR-93) is upregulated in the maternal plasma of patients with PE. However, the functional role of miR-93 in PE remains unknown. Here, we identified that miR-93 inhibits trophoblastic invasion, which is correlated with PE development. In immortalized trophoblast cell lines, transwell assay showed that miR-93 mimics significantly inhibited the migration and invasion of immortalized trophoblast cells, whereas miR-93 inhibitors significantly promoted cell migration and invasion. Moreover, luciferase assays confirmed that miR-93 directly bound to the 3' untranslated region of matrix metalloproteinase-2 (MMP-2), and western blotting showed that miR-93 suppressed the expression of MMP-2 at the protein levels. This study indicated that miR-93 inhibits MMP-2 and reduces migration and invasion of immortalized trophoblast cells. Thus, miR-93 may represent a potential therapeutic target for PE intervention. (C) 2017 Elsevier Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Sosa, SEY AU - Flores-Pliego, A AU - Espejel-Nuñez, A AU - Medina-Bastidas, D AU - Vadillo-Ortega, F AU - Zaga-Clavellina, V AU - Estrada-Gutierrez, G TI - New insights into the role of matrix metalloproteinases in preeclampsia JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 18 PY - 2017 IS - 7 SN - 1661-6596 DO - 10.3390/ijms18071448 UR - https://m2.mtmt.hu/api/publication/26853070 ID - 26853070 LA - English DB - MTMT ER -