@article{MTMT:34744297, title = {Mathematical Analysis of Streptococcus Suis Infection in Pig-Human Population by Riemann-Liouville’s Fractional Operator}, url = {https://m2.mtmt.hu/api/publication/34744297}, author = {Alqahtani, A.M. and Mishra, M.N.}, doi = {10.18576/pfda/100112}, journal-iso = {Progr. Fract. Differ. Appl.}, journal = {Progress in Fractional Differentiation and Applications}, volume = {10}, unique-id = {34744297}, issn = {2356-9336}, abstract = {We addressed a mathematical model for Streptococcus Suis, an uncommon infectious illness that is contaminated, in the current paper. The pig infection that eventually infects people is the source of the illness Streptococcus suis. This disease manifests in a highly severe form in human transmissions, with the potential for both significant sickness and death. Seven population groups (some from human populations and some from pig populations as well) have been examined in this article. We have analysed and studied the given mathematical model of the illness using the Riemann Liouville’s fractional derivative and Laplace transformation. Using a graphical representation of the solutions, we have also confirmed their existence and oneness. © 2024 NSP Natural Sciences Publishing Cor}, keywords = {STABILITY ANALYSIS; mathematical model; Existence and uniqueness; Streptococcus suis; Laplace transformation; Riemann-Liouville’s fractional derivative}, year = {2024}, eissn = {2356-9344}, pages = {119-135} } @article{MTMT:34648842, title = {Screening of Streptococcus suis in swine workers of selected states in}, url = {https://m2.mtmt.hu/api/publication/34648842}, author = {Lee, Chee Yien and Zakaria, Zunita and Selvarajah, Gayathri Thevi and Mustaffa-Kamal, Farina and Voon, Kenny Gah Leong and Fong, Michelle Wai Cheng and Ooi, Peck Toung}, doi = {10.14202/vetworld.2024.1-7}, journal-iso = {VETERINARY WORLD}, journal = {VETERINARY WORLD}, volume = {17}, unique-id = {34648842}, issn = {0972-8988}, keywords = {human; CARRIER; Streptococcus suis; high-risk occupation}, year = {2024}, eissn = {2231-0916}, pages = {1-7} } @article{MTMT:34520474, title = {Insights into the potential pathogenic bacteria and their interactions with meteorology and atmospheric pollution conditions examined during summer and winter in Xi’an, China}, url = {https://m2.mtmt.hu/api/publication/34520474}, author = {Yang, L. and Yang, Y. and Wei, J. and Wang, T. and Li, L. and Shen, Z.}, doi = {10.1080/02786826.2023.2283480}, journal-iso = {AEROSOL SCI TECH}, journal = {AEROSOL SCIENCE AND TECHNOLOGY}, volume = {58}, unique-id = {34520474}, issn = {0278-6826}, abstract = {Bioaerosols can influence the atmospheric environment and human health. In this study, we investigated the diurnal variations of bioaerosols and the bacterial communities in PM2.5 during summer and winter. The fungal concentration (795.7 CFU/m3) was higher in winter, and bacteria concentration (315.9 CFU/m3) was higher in summer. For TAMs, summer concentration (19.9 × 106 cell/m3) was 1.4 times higher than that in winter. The bacterial and fungal concentrations were the lowest in the afternoon, along with the highest level of ozone, which damaged microbial DNA and inhibited their growth. The concentration of TAMs exhibited a bimodal distribution with two peaks coinciding with morning and evening traffic rush hours. In winter, the number of bacterial operational taxonomic units was 3 times higher than that in summer. Proteobacteria peaked in the evening (48.5%), and Actinobacteria (18%) appeared at the same time in summer. During summertime, the morning and afternoon genera were different from the evening and night genera, while the composition of bacterial communities was stable in winter. Four potentially pathogenic bacteria (Pseudomonas, Serratia, Bacteroides, and Streptococcus) were detected in summer, but only one (Acinetobacter) was detected in winter. The Mantel test revealed that the abundance of pathogenic bacteria were influenced by the relative humidity, ozone level, and carbon monoxide level in summer, while it was more complicated for the interaction role among high PM2.5 levels, oxidative potential, as well as meteorological parameters with pathogenic bacteria in winter. This study provides important insights into the effects of bioaerosol pollution that could affect human health. Copyright © 2023 American Association for Aerosol Research. © 2023 American Association for Aerosol Research.}, keywords = {ARTICLE; BACTERIA; air pollution; air pollution; AEROSOLS; aerosol; OZONE; OZONE; WINTER; Virulence; clinical examination; FUNGUS; nonhuman; night; circadian rhythm; CHINA; STREPTOCOCCUS; bacteroides; carbon monoxide; carbon monoxide; bacterium; ATMOSPHERIC POLLUTION; PSEUDOMONAS; MICROBIAL COMMUNITY; BACTERIAL COMMUNITY; PROTEOBACTERIA; Species richness; acinetobacter; Actinobacteria; oxidation reduction potential; bacterial growth; seasonal variation; summer; clinical evaluation; meteorological phenomena; species diversity; colony forming unit; bacterial DNA; agar; Atmospheric humidity; diurnal variation; Human health; meteorology; Mantel test; relative humidity; PM 2.5; Pathogenic bacterium; condition; Bioaerosols; Operational Taxonomic Unit; concentration (parameter); Particulate matter 2.5; Atmospheric environment; respiratory droplets and aerosols; Serratia (bacterium); Shanna Ratnesar-Shumate; Shanna Ratnesar-Shumate}, year = {2024}, eissn = {1521-7388}, pages = {26-38} } @article{MTMT:33950379, title = {Antibiotic Resistance Patterns and Molecular Characterization of Streptococcus suis Isolates from Swine and Humans in China}, url = {https://m2.mtmt.hu/api/publication/33950379}, author = {Wang, Chang-Zhen and Wang, Min-Ge and Chu, Yue-Fei and Sun, Ruan-Yang and Li, Jian-Guo and Li, Xian-An and Sun, Jian and Liu, Ya-Hong and Zhou, Yu-Feng and Liao, Xiao-Ping}, doi = {10.1128/spectrum.00309-23}, journal-iso = {MICROBIOL SPEC}, journal = {MICROBIOLOGY SPECTRUM}, volume = {11}, unique-id = {33950379}, issn = {2165-0497}, abstract = {S. suis is a serious zoonotic pathogen. In this study, we investigated the epidemiological and molecular characteristics of 96 S. suis isolates from 10 different provinces of China from 2008 to 2019.Streptococcus suis is a zoonotic pathogen that causes disease in humans after exposure to infected pigs or pig-derived food products. In this study, we examined the serotype distribution, antimicrobial resistance phenotypes and genotypes, integrative and conjugative elements (ICEs), and associated genomic environments of S. suis isolates from humans and pigs in China from 2008 to 2019. We identified isolates of 13 serotypes, predominated by serotype 2 (40/96; 41.7%), serotype 3 (10/96; 10.4%), and serotype 1 (6/96; 6.3%). Whole-genome sequencing analysis revealed that these isolates possessed 36 different sequence types (STs), and ST242 and ST117 were the most prevalent. Phylogenetic analysis revealed possible animal and human clonal transmission, while antimicrobial susceptibility testing indicated high-level resistance to macrolides, tetracyclines, and aminoglycosides. These isolates carried 24 antibiotic resistance genes (ARGs) that conferred resistance to 7 antibiotic classes. The antibiotic resistance genotypes were directly correlated with the observed phenotypes. We also identified ICEs in 10 isolates, which were present in 4 different genetic environments and possessed differing ARG combinations. We also predicted and confirmed by PCR analysis the existence of a translocatable unit (TU) in which the oxazolidinone resistance gene optrA was flanked by IS1216E elements. One-half (5/10) of the ICE-carrying strains could be mobilized by conjugation. A comparison of the parental recipient with an ICE-carrying transconjugant in a mouse in vivo thigh infection model indicated that the ICE strain could not be eliminated with tetracycline treatment. S. suis therefore poses a significant challenge to global public health and requires continuous monitoring, especially for the presence of ICEs and associated ARGs that can be transferred via conjugation.IMPORTANCE S. suis is a serious zoonotic pathogen. In this study, we investigated the epidemiological and molecular characteristics of 96 S. suis isolates from 10 different provinces of China from 2008 to 2019. A subset of these isolates (10) carried ICEs that were able to be horizontally transferred among isolates of different S. suis serotypes. A mouse thigh infection model revealed that ICE-facilitated ARG transfer promoted resistance development. S. suis requires continuous monitoring, especially for the presence of ICEs and associated ARGs that can be transferred via conjugation.}, keywords = {serotype; Ices; S; WGS; transferability; suis; integrative and conjugative elements}, year = {2023}, eissn = {2165-0497}, orcid-numbers = {Sun, Jian/0000-0002-4209-7555} } @article{MTMT:34520475, title = {Aerococcus viridans Phage Lysin AVPL Had Lytic Activity against Streptococcus suis in a Mouse Bacteremia Model}, url = {https://m2.mtmt.hu/api/publication/34520475}, author = {Xi, H. and Fu, Y. and Chen, C. and Feng, X. and Han, W. and Gu, J. and Ji, Y.}, doi = {10.3390/ijms242316670}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {24}, unique-id = {34520475}, issn = {1661-6596}, abstract = {Streptococcus suis (S. suis) is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in fatal human infections. The widespread prevalence of multidrug-resistant S. suis strains and the decline in novel antibiotic candidates have necessitated the development of alternative antimicrobial agents. In this study, AVPL, the Aerococcus viridans (A. viridans) phage lysin, was found to exhibit efficient bactericidal activity and broad lytic activity against multiple serotypes of S. suis. A final concentration of 300 μg/mL AVPL reduced S. suis counts by 4–4.5 log10 within 1 h in vitro. Importantly, AVPL effectively inhibited 48 h S. suis biofilm formation and disrupted preformed biofilms. In a mouse model, 300 μg/mouse AVPL protected 100% of mice from infection following the administration of lethal doses of multidrug-resistant S. suis type 2 (SS2) strain SC19, reduced the bacterial load in different organs, and effectively alleviated inflammation and histopathological damage in infected mice. These data suggest that AVPL is a valuable candidate antimicrobial agent for treating S. suis infections. © 2023 by the authors.}, keywords = {Animals; Humans; MICE; SWINE; MOUSE; human; animal; PIG; microbiology; Disease Models, Animal; disease model; Biofilm; BACTEREMIA; BACTEREMIA; BACTEREMIA; bacteriophage; Streptococcal Infections; Streptococcus infection; Bacteriophages; Streptococcus suis; Streptococcus suis; Streptococcus suis; phage lysin; Aerococcus viridans; Aerococcus; Aerococcus}, year = {2023}, eissn = {1422-0067} } @article{MTMT:33098068, title = {A Streptococcus suis infection causing pneumonia in Indonesia: A case report}, url = {https://m2.mtmt.hu/api/publication/33098068}, author = {Cahyaningtyas, Cheria and Rahmawati, Rahmawati and Muslich, Lisa Tenriesa and Hamid, Firdaus and Sultan, Andi Rofian and Sjahril, Rizalinda and Massi, Muhammad Nasrum and Hatta, Mochammad and Madjid, Baedah}, doi = {10.1016/j.radcr.2022.08.030}, journal-iso = {Radiology Case Reports}, journal = {Radiology Case Reports}, volume = {17}, unique-id = {33098068}, issn = {1930-0433}, year = {2022}, pages = {4337-4340}, orcid-numbers = {Cahyaningtyas, Cheria/0000-0002-5368-9978} } @article{MTMT:32774964, title = {The mathematical model for streptococcus suis infection in pig-human population with humidity effect}, url = {https://m2.mtmt.hu/api/publication/32774964}, author = {Chaiya, I. and Trachoo, K. and Nonlaopon, K. and Prathumwan, D.}, doi = {10.32604/cmc.2022.021856}, journal-iso = {CMC-COMPUT MATER CON}, journal = {CMC-COMPUTERS MATERIALS & CONTINUA}, volume = {71}, unique-id = {32774964}, issn = {1546-2218}, abstract = {In this paper, we developed a mathematical model for Streptococcus suis, which is an epidemic by considering the moisture that affects the infection. The disease is caused by Streptococcus suis infection found in pigs which can be transmitted to humans. The patients of Streptococcus suis were generally found in adults males and the elderly who contacted pigs or who ate uncooked pork. In human cases, the infection can cause a severe illness and death. This disease has an impact to the financial losses in the swine industry. In the development of models for this disease, we have divided the population into 7 related groups which are susceptible pig compartment, infected pig compartment, quarantined pig compartment, recovered pig compartment, susceptible human compartment, infected human compartment, and recovered human compartment. After that, we use this model and a quarantine strategy to analyze the spread of the infection. In addition, the basic reproduction number R0 is determined by using the next-generation matrixwhich can analyze the stability of the model. The numerical simulations of the proposed model are illustrated to confirm the results from theorems. The results showed that there is an effect from moisture to the disease transmission.When themoisture increases the disease infection also increases. © 2022 Tech Science Press. All rights reserved.}, keywords = {infectious disease; infectious disease; cell proliferation; DISEASES; Soil moisture; STABILITY ANALYSIS; mathematical model; MAMMALS; disease transmission; moisture; Human population; LOSSES; Basic Reproduction Number; Financial loss; Streptococcus suis; reproduction number; REPRODUCTION NUMBERS; Humidity effects; Stability analyze; Streptococcus sui}, year = {2022}, eissn = {1546-2226}, pages = {2981-2998} } @article{MTMT:33025352, title = {Genomic characterization of Streptococcus parasuis, a close relative of Streptococcus suis and also a potential opportunistic zoonotic pathogen}, url = {https://m2.mtmt.hu/api/publication/33025352}, author = {Guo, Genglin and Wang, Zhuohao and Li, Quan and Yu, Yanfei and Li, Yubao and Tan, Zhongming and Zhang, Wei}, doi = {10.1186/s12864-022-08710-6}, journal-iso = {BMC GENOMICS}, journal = {BMC GENOMICS}, volume = {23}, unique-id = {33025352}, issn = {1471-2164}, year = {2022}, eissn = {1471-2164} } @article{MTMT:32612865, title = {Association of Common Zoonotic Pathogens With Concentrated Animal Feeding Operations}, url = {https://m2.mtmt.hu/api/publication/32612865}, author = {Guo, Yaqiong and Ryan, Una and Feng, Yaoyu and Xiao, Lihua}, doi = {10.3389/fmicb.2021.810142}, journal-iso = {FRONT MICROBIOL}, journal = {FRONTIERS IN MICROBIOLOGY}, volume = {12}, unique-id = {32612865}, issn = {1664-302X}, year = {2022}, eissn = {1664-302X} } @article{MTMT:32778362, title = {Airborne microbial community structure and potential pathogen identification across the PM size fractions and seasons in the urban atmosphere}, url = {https://m2.mtmt.hu/api/publication/32778362}, author = {Jiang, Shaoyi and Sun, Bowen and Zhu, Renbin and Che, Chenshuai and Ma, Dawei and Wang, Runfang and Dai, Haitao}, doi = {10.1016/j.scitotenv.2022.154665}, journal-iso = {SCI TOTAL ENVIRON}, journal = {SCIENCE OF THE TOTAL ENVIRONMENT}, volume = {831}, unique-id = {32778362}, issn = {0048-9697}, year = {2022}, eissn = {1879-1026} } @article{MTMT:33098094, title = {From Farm to Fork: Streptococcus suis as a Model for the Development of Novel Phage-Based Biocontrol Agents}, url = {https://m2.mtmt.hu/api/publication/33098094}, author = {Osei, Emmanuel Kuffour and Mahony, Jennifer and Kenny, John G.}, doi = {10.3390/v14091996}, journal-iso = {VIRUSES-BASEL}, journal = {VIRUSES}, volume = {14}, unique-id = {33098094}, abstract = {Bacterial infections of livestock threaten the sustainability of agriculture and public health through production losses and contamination of food products. While prophylactic and therapeutic application of antibiotics has been successful in managing such infections, the evolution and spread of antibiotic-resistant strains along the food chain and in the environment necessitates the development of alternative or adjunct preventive and/or therapeutic strategies. Additionally, the growing consumer preference for “greener” antibiotic-free food products has reinforced the need for novel and safer approaches to controlling bacterial infections. The use of bacteriophages (phages), which can target and kill bacteria, are increasingly considered as a suitable measure to reduce bacterial infections and contamination in the food industry. This review primarily elaborates on the recent veterinary applications of phages and discusses their merits and limitations. Furthermore, using Streptococcus suis as a model, we describe the prevalence of prophages and the anti-viral defence arsenal in the genome of the pathogen as a means to define the genetic building blocks that are available for the (synthetic) development of phage-based treatments. The data and approach described herein may provide a framework for the development of therapeutics against an array of bacterial pathogens.}, year = {2022}, eissn = {1999-4915}, orcid-numbers = {Osei, Emmanuel Kuffour/0000-0001-8188-8934; Mahony, Jennifer/0000-0001-5846-6303; Kenny, John G./0000-0003-2994-6169} } @article{MTMT:33268717, title = {Study of Transmission Dynamics of Streptococcus suis Infection Mathematical Model between Pig and Human under ABC Fractional Order Derivative}, url = {https://m2.mtmt.hu/api/publication/33268717}, author = {Prathumwan, Din and Chaiya, Inthira and Trachoo, Kamonchat}, doi = {10.3390/sym14102112}, journal-iso = {SYMMETRY-BASEL}, journal = {SYMMETRY (BASEL)}, volume = {14}, unique-id = {33268717}, abstract = {In this paper, a mathematical model for Streptococcus suis infection is improved by using the fractional order derivative. The modified model also investigates the transmission between pigs and humans. The proposed model can classify the pig population density into four classes, which are pig susceptible class, pig infectious class, pig quarantine class, and pig recovery class. Moreover, the human population density has been separated into three classes, these are human susceptible class, human infectious class, and human recovery class. The spread of the infection is analyzed by considering the contact between humans and pigs. The basic reproduction number (R-0), the infectious indicator, is carried out using the next generation matrix. The disease-free equilibrium is locally asymptotically stable if R-0 < 1, and the endemic equilibrium is locally asymptotically stable if R-0 > 1. The theoretical analyses of the fractional order derivative model, existence and uniqueness, have been proposed. The numerical examples were illustrated to support the proposed stability theorems. The results show that the fractional order derivative model provides the various possible solution trajectories with different fractional orders for the same parameters. In addition, transmission between pigs and humans resulted in the spread of Streptococcus suis infection.}, keywords = {STABILITY ANALYSIS; epidemic; Streptococcus suis; endemic disease model; Atagana-Baleanu-Caputo fractional order derivative}, year = {2022}, eissn = {2073-8994}, orcid-numbers = {Prathumwan, Din/0000-0002-5485-8956} } @article{MTMT:31907812, title = {Prevalence and Traits of Mobile Colistin Resistance Gene Harbouring Isolates from Different Ecosystems in Africa}, url = {https://m2.mtmt.hu/api/publication/31907812}, author = {Anyanwu, M.U. and Okpala, C.O.R. and Chah, K.F. and Shoyinka, V.S.}, doi = {10.1155/2021/6630379}, journal-iso = {BIOMED RES INT}, journal = {BIOMED RESEARCH INTERNATIONAL}, volume = {2021}, unique-id = {31907812}, issn = {2314-6133}, abstract = {The mobile colistin resistance (mcr) gene threatens the efficacy of colistin (COL), a last-line antibiotic used in treating deadly infections. For more than six decades, COL is used in livestock around the globe, including Africa. The use of critically important antimicrobial agents, like COL, is largely unregulated in Africa, and many other factors militate against effective antimicrobial stewardship in the continent. Currently, ten mcr genes (mcr-1 to mcr-10) have been described. In Africa, mcr-1, mcr-2, mcr-3, mcr-5, mcr-8, and mcr-9 have been detected in isolates from humans, animals, foods of animal origin, and the environment. These genes are harboured by Escherichia coli, Klebsiella, Salmonella, Citrobacter, Enterobacter, Pseudomonas, Aeromonas, Alcaligenes, and Acinetobacter baumannii isolates. Different conjugative and nonconjugative plasmids form the backbone for mcr in these isolates; however, mcr-1 and mcr-3 have also been integrated into the chromosome of some African strains. Insertion sequences (ISs) (especially ISApl1), either located upstream or downstream of mcr, class 1 integrons, and transposons, are drivers of mcr in Africa. Genes coding multi/extensive drug resistance and virulence are colocated with mcr on plasmids in African strains. Transmission of mcr to/among African strains is nonclonal. Contact with mcr-habouring reservoirs, the consumption of contaminated foods of animal/plant origin or fluid, animal-/plant-based food trade and travel serve as exportation, importation, and transmission routes of mcr gene-containing bacteria in Africa. Herein, the current status of plasmid-mediated COL resistance in humans, food-producing animals, foods of animal origin, and environment in Africa is discussed. © 2021 Madubuike Umunna Anyanwu et al.}, year = {2021}, eissn = {2314-6141} } @article{MTMT:32155648, title = {Peptidomics Analysis of Virulent Peptides Involved in Streptococcus suis Pathogenesis}, url = {https://m2.mtmt.hu/api/publication/32155648}, author = {Chadaporn, Chaiden and Janthima, Jaresitthikunchai and Narumon, Phaonakrop and Sittiruk, Roytrakul and Anusak, Kerdsin and Suphachai, Nuanualsuwan}, doi = {10.3390/ani11092480}, journal-iso = {ANIMALS-BASEL}, journal = {ANIMALS}, volume = {11}, unique-id = {32155648}, year = {2021}, eissn = {2076-2615} } @article{MTMT:32173694, title = {Foodborne Botulism in Western Romania: Ten Years’ Experience at a Tertiary Infectious Disease Hospital}, url = {https://m2.mtmt.hu/api/publication/32173694}, author = {Iosif, Marincu and Felix, Bratosin and Iulia, Vidican and Bianca, Cerbu and Oana, Suciu and Mirela, Turaiche and Livius, Tirnea and Madalina, Timircan}, doi = {10.3390/healthcare9091149}, journal-iso = {HEALTHCARE-BASEL}, journal = {HEALTHCARE}, volume = {9}, unique-id = {32173694}, year = {2021}, eissn = {2227-9032} } @article{MTMT:32089994, title = {Investigation of the Genomic and Pathogenic Features of the Potentially Zoonotic Streptococcus parasuis}, url = {https://m2.mtmt.hu/api/publication/32089994}, author = {Jianping, Wang and Xueli, Yi and Pujun, Liang and Yuanmeihui, Tao and Yan, Wang and Dong, Jin and Bin, Luo and Jing, Yang and Han, Zheng}, doi = {10.3390/pathogens10070834}, journal-iso = {PATHOGENS}, journal = {PATHOGENS}, volume = {10}, unique-id = {32089994}, year = {2021}, eissn = {2076-0817} } @article{MTMT:31907810, title = {A novel bicyclic 2,4-diaminopyrimidine inhibitor of Streptococcus suis dihydrofolate reductase}, url = {https://m2.mtmt.hu/api/publication/31907810}, author = {Songsungthong, W. and Prasopporn, S. and Bohan, L. and Srimanote, P. and Leartsakulpanich, U. and Yongkiettrakul, S.}, doi = {10.7717/peerj.10743}, journal-iso = {PEERJ}, journal = {PEERJ}, volume = {9}, unique-id = {31907810}, issn = {2167-8359}, abstract = {Streptococcus suis is a Gram-positive bacterial pathogen of pigs and an emerging zoonotic pathogen. It has become increasingly resistant to multiple classes of antibiotics. New drug candidates and knowledge of their targets are needed to combat antibiotic- resistant S. suis. In this study, the open-source Pathogen Box compound library was screened. Thirty hits that effectively inhibited S. suis growth at 10 mM were identified. Among the most potent hits, MMV675968 (a diaminoquinazoline analog) was shown to target S. suis dihydrofolate reductase (SsDHFR) via (1) growth inhibition of an E. coli surrogate whose growth is dependent on exogenously expressed SsDHFR and (2) inhibition of in vitro SsDHFR activity. Thymidine supplement is able to reverse growth inhibition by MMV675968 in both E. coli surrogate and S. suis, indicating that a thymidine-related pathway is a major target of MMV675968. Comparison of MMV675968 with seven DHFR inhibitors representing different core structures revealed that bicyclic 2,4-diaminopyrimidines with long and flexible side chains are highly effective in inhibiting SsDHFR and S. suis growth. MMV675968 and related compounds thus may serve as starting points for developing antibiotics against drug resistant S. suis. Copyright 2021 Songsungthong et al.}, keywords = {antibiotics; DRUG DISCOVERY; Screening; growth inhibition; dihydrofolate reductase; Drug resistant; Streptococcus suis; Pathogen Box; Antifolate; Diaminopyrimidine}, year = {2021}, eissn = {2167-8359} } @article{MTMT:31829092, title = {Genome-wide analysis of the synonymous codon usage pattern of Streptococcus suis}, url = {https://m2.mtmt.hu/api/publication/31829092}, author = {Xu, Q. and Chen, H. and Sun, W. and Zhu, D. and Zhang, Y. and Chen, J.-L. and Chen, Y.}, doi = {10.1016/j.micpath.2021.104732}, journal-iso = {MICROB PATHOGENESIS}, journal = {MICROBIAL PATHOGENESIS}, volume = {150}, unique-id = {31829092}, issn = {0882-4010}, abstract = {Streptococcus suis (S. suis) is a gram-positive coccus that causes disease in humans and animals. The codon usage pattern of bacteria reveals a range of evolutionary changes that assist them to enhance tolerance to environments. To better understand the genetic features during the evolution of S. suis, we performed codon usage analysis. Nine pathogenic strains of different serotypes and different geographical distribution were analyzed to better understand the differences in their evolutionary process. Nucleotide compositions and relative synonymous codon usage (RSCU) analysis revealed that A/T-ending codons are dominant in S. suis. Neutrality analysis, correspondence analysis and ENC-plot results revealed that natural selection is the predominant element prompting codon usage. Cluster analysis based on RSCU was roughly consistent with the dendrogram rooted genomic BLAST analysis. Comparison of synonymous codon usage pattern between S. suis and susceptible hosts (H. sapiens and S. scrofa) revealed that the codon usage of S. suis is separated from the synonymous codon usage of susceptible hosts. The CAI values implied that S. suis includes a series of predicted highly expressed coding sequences contained in metabolism and transcriptional regulation, revealing the necessity of this pathogen to deal with various environmental conditions. The study of codon usage in S. suis may provide evidence involving the molecular evolution of bacteria and a better understanding of evolutionary relationships between S. suis and its corresponding hosts. © 2021}, keywords = {natural selection; Streptococcus suis; codon usage; Evolutionary process}, year = {2021}, eissn = {1096-1208} } @article{MTMT:34520479, title = {Molecular epidemiological methods of Streptococcus suis. 猪链球菌分子流行病学研究方法}, url = {https://m2.mtmt.hu/api/publication/34520479}, author = {Yang, Shixin and Bian, Chen and Wu, Zongfu}, journal-iso = {WEISHENGWU XUEBAO / ACTA MICROBIOL SIN}, journal = {WEISHENGWU XUEBAO / ACTA MICROBIOLOGICA SINICA}, volume = {61}, unique-id = {34520479}, issn = {0001-6209}, abstract = {Streptococcus suis is an important bacterial pathogen for pigs.It can cause meningitis,sepsis and arthritis in pigs,leading to severe economic losses to the pig industry.In addition,S.suis can also infect humans and is considered a zoonotic pathogen.In order to better prevent and control the disease caused by S.suis,it is necessary to use molecular epidemiological methods to elucidate the epidemiological characteristics of S.suis,including virulence typing,temporal and spatial distribution,transmission route,source of infection,and genetic determinants of transmission.At present,there are several commonly used methods including multilocus sequence typing,pulsed-field gel electrophoresis,whole genome sequencing,and PCR-based methods.This article introduces the principles of the above-mentioned methods and the application examples in the epidemiology of S.suis,and analyzes their advantages and disadvantages,which contributes to better revealing the epidemiological characteristics of S.suis,and providing a reference for formulating prevention and control strategies for the disease caused by S.suis.}, year = {2021}, pages = {3928-3936} }