TY - JOUR AU - Mekky, Alsayed E. AU - Abdelaziz, Ahmed E. M. AU - Youssef, Fady Sayed AU - Elaskary, Shymaa A. AU - Shoun, Aly A. AU - Alwaleed, Eman A. AU - Gaber, Mahmoud Ali AU - Al-Askar, Abdulaziz A. AU - Alsamman, Alhadary M. AU - Yousef, Abdullah AU - AbdElgayed, Gehad AU - Suef, Reda A. AU - Selim, Mohamed A AU - Saied, Ebrahim AU - Khedr, Mohamed TI - Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles JF - MEDICINA-LITHUANIA J2 - MED LITH VL - 60 PY - 2024 IS - 3 PG - 31 SN - 1010-660X DO - 10.3390/medicina60030515 UR - https://m2.mtmt.hu/api/publication/34753137 ID - 34753137 N1 - Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt Botany and Microbiology Department, Faculty of Science, Port-Said University, 23 December Street, P.O. Box 42522, Port-Said, 42522, Egypt Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt Medical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Shibin El-Kom, 32511, Egypt Microbiology and Immunology Department, Faculty of Pharmacy, El Salehey El Gadida University, El Saleheya El Gadida, 44813, Egypt Botany and Microbiology Department, Faculty of Science, South Valley University, Qena, 83523, Egypt Botany and Microbiology Department, Faculty of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia Basic & Medical Sciences Department, Faculty of Dentistry, Alryada University for Science & Technology, Sadat, 32897, Egypt Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, Antwerp, 2020, Belgium Export Date: 26 April 2024 Correspondence Address: Mekky, A.E.; Botany and Microbiology Department, Nasr City, Egypt; email: alsayedessam@azhar.edu.eg Chemicals/CAS: hydrogen peroxide, 7722-84-1; polyphenol, 37331-26-3; silver, 7440-22-4; tannin, 1401-55-4; Adhesins, Bacterial; Anti-Bacterial Agents; Anti-Infective Agents; Anti-Inflammatory Agents; Antioxidants; fibronectin-binding proteins, bacterial; Hydrogen Peroxide; Polyphenols; Silver; Tannic Acid Funding details: King Saud University, KSU Funding text 1: The authors extend their appreciation to the researchers supporting project number (RSP2024R505), King Saud University, Riyadh, Saudi Arabia. AB - Background and Objectives: Urinary tract infections [UTIs] are considered the third most known risk of infection in human health around the world. There is increasing appreciation for the pathogenicity of Gram-positive and Gram-negative strains in UTIs, aside from fungal infection, as they have numerous virulence factors. Materials and Methods: In this study, fifty urine samples were collected from patients suffering from UTI. Among the isolates of UTI microbes, six isolates were described as MDR isolates after an antibiotic susceptibility test carried out using ten different antibiotics. An alternative treatment for microbial elimination involved the use of biosynthesized silver nanoparticles (AgNPs) derived from Solanum lycopersicum [S. cumin]. Results: The sizes and shapes of AgNPs were characterized through TEM imaging, which showed spherical particles in a size range of 35–80 nm, of which the average size was 53 nm. Additionally, the silver nanoparticles (AgNPs) demonstrated inhibitory activity against Staphylococcus aureus (OR648079), exhibiting a 31 mm zone of inhibition at a minimum inhibitory concentration (MIC) of 4 mg/mL and a minimum bactericidal concentration (MBC) of 8 mg/mL. This was followed by Aspergillus niger (OR648075), which showed a 30 mm inhibition zone at an MIC of 16 mg/mL and a minimum fungicidal concentration (MFC) of 32 mg/mL. Then, Enterococcus faecalis (OR648078), Klebsiella pneumoniae (OR648081), and Acinetobacter baumannii (OR648080) each displayed a 29 mm zone of inhibition at an MIC of 8 mg/mL and an MBC of 16 mg/mL. The least inhibition was observed against Candida auris (OR648076), with a 25 mm inhibition zone at an MIC of 16 mg/mL and an MFC of 32 mg/mL. Furthermore, AgNPs at different concentrations removed DPPH and H2O2 at an IC50 value of 13.54 μg/mL. Also, AgNPs at 3 mg/mL showed remarkable DNA fragmentation in all bacterial strains except Enterococcus faecalis. The phytochemical analysis showed the presence of different active organic components in the plant extract, which concluded that rutin was 88.3 mg/g, garlic acid was 70.4 mg/g, and tannic acid was 23.7 mg/g. Finally, AgNPs concentrations in the range of 3–6 mg/mL showed decreased expression of two of the fundamental genes necessary for biofilm formation within Staphylococcus aureus, fnbA (6 folds), and Cna (12.5 folds) when compared with the RecA gene, which decreased by one-fold when compared with the control sample. These two genes were submitted with NCBI accession numbers [OR682119] and [OR682118], respectively. Conclusions: The findings from this study indicate that biosynthesized AgNPs from Solanum lycopersicum exhibit promising antimicrobial and antioxidant properties against UTI pathogens, including strains resistant to multiple antibiotics. This suggests their potential as an effective alternative treatment for UTIs. Further research is warranted to fully understand the mechanisms of action and to explore the therapeutic applications of these nanoparticles in combating UTIs. LA - English DB - MTMT ER - TY - CHAP AU - Seku, Kondaiah AU - Hussaini, Syed Sulaiman AU - Bhagavanth Reddy, G. AU - Radha Krishna Reddy, M. TI - Silver-based biofungicides for the suppression of pathogenic fungi in agriculture fields T2 - Nanofungicides PB - Elsevier SN - 9780323953054 PY - 2024 SP - 169 EP - 194 PG - 26 DO - 10.1016/B978-0-323-95305-4.00009-1 UR - https://m2.mtmt.hu/api/publication/34549940 ID - 34549940 LA - English DB - MTMT ER - TY - JOUR AU - Lean, Jo Sze AU - Wan Mohamad Ali, Wan Najdah AU - Ahmad, Rohani AU - Mohamed Nor, Zurainee AU - Wong, Ching Lee AU - Ng, Jeck Fei TI - Size-tunable Sargassum polycystum mediated synthesis of silver nanoparticles and its larvicidal effect on Aedes aegypti JF - JOURNAL OF APPLIED PHYCOLOGY J2 - J APPL PHYCOL PY - 2023 SN - 0921-8971 DO - 10.1007/s10811-023-02997-y UR - https://m2.mtmt.hu/api/publication/33924978 ID - 33924978 LA - English DB - MTMT ER - TY - JOUR AU - Mačák, Lívia AU - Velgosová, Oksana AU - Múdra, Erika AU - Vojtko, Marek AU - Dolinská, Silvia TI - Transfer of AgNPs’ Anti-Biofilm Activity into the Nontoxic Polymer Matrix JF - POLYMERS J2 - POLYMERS-BASEL VL - 15 PY - 2023 IS - 5 SP - 1238 SN - 2073-4360 DO - 10.3390/polym15051238 UR - https://m2.mtmt.hu/api/publication/33681041 ID - 33681041 AB - A biological method was successfully applied to synthesize spherical silver nanoparticles (AgNPs) while using the extract of lavender (Ex-L) (lat. Lavandula angustifolia) as the reducing and stabilizing agent. The produced nanoparticles were spherical with an average size of 20 nm. The AgNPs’ synthesis rate confirmed the extract’s excellent ability to reduce silver nanoparticles from the AgNO3 solution. The presence of good stabilizing agents was confirmed by the excellent stability of the extract. Nanoparticles’ shapes and sizes did not change. UV-Vis absorption spectrometry, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used to characterize the silver nanoparticles. The silver nanoparticles were incorporated into the PVA polymer matrix by the “ex situ” method. The polymer matrix composite with AgNPs was prepared in two ways: as a composite film and nanofibers (nonwoven textile). The anti-biofilm activity of AgNPs and the ability of AgNPs to transfer toxic properties into the polymer matrix were proved. LA - English DB - MTMT ER - TY - JOUR AU - Maciel, Antônio Auberson Martins AU - Cunha, Francisco Afrânio AU - Freire, Tiago Melo AU - de Menezes, Fernando Lima AU - Fechine, Lillian Maria Uchoa Dutra AU - Rocha, Janaina Sobreira AU - de Cássia Carvalho Barbosa, Rita AU - Martins, Roxeane Teles AU - da Conceição dos Santos Oliveira Cu, Maria AU - Santos-Oliveira, Ralph AU - Queiroz, Maria Veraci Oliveira AU - Fechine, Pierre Basílio Almeida TI - Development and evaluation of an anti-candida cream based on silver nanoparticles JF - 3 BIOTECH J2 - 3 BIOTECH VL - 13 PY - 2023 IS - 11 SP - 1 SN - 2190-572X DO - 10.1007/s13205-023-03776-9 UR - https://m2.mtmt.hu/api/publication/34179978 ID - 34179978 N1 - Advanced Materials Chemistry Group (GQMat), Department of Analytical Chemistry and Physical Chemistry, Federal University of Ceará (UFC), Pici Campus, 12100, CE, Fortaleza, 60451-970, Brazil Departament of Clinical and Toxicological Analysis, Federal University of Ceará (UFC), Capitão Francisco Pedro Street, 1210, Rodolfo Teófilo, CE, Fortaleza, 60270-430, Brazil State University of Ceará. Graduate Program in Clinical Care in Nursing and Health, CE, Fortaleza, Brazil Princesa do Oeste College, CE, Crateús, Brazil Laboratory of Nanoradiopharmacy and Synthesis of New Radiopharmaceuticals, Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, RJ, Rio de Janeiro, 21941906, Brazil Laboratory of Nanoradiopharmacy and Strategic Biomaterials, Zona Oeste State University, RJ, Rio de Janeiro, 220000, Brazil Export Date: 30 January 2024 Correspondence Address: Fechine, P.B.A.; Advanced Materials Chemistry Group (GQMat), Pici Campus, 12100, CE, Brazil; email: fechine@ufc.br LA - English DB - MTMT ER - TY - JOUR AU - Mares-Briones, Fabian AU - Higareda, América AU - Lopez-Miranda, Jose Luis AU - Mendoza-Cruz, Rubén AU - Esparza, Rodrigo TI - Bimetallic AgPt Nanoalloys as an Electrocatalyst for Ethanol Oxidation Reaction: Synthesis, Structural Analysis, and Electro-Catalytic Activity JF - NANOMATERIALS J2 - NANOMATERIALS-BASEL VL - 13 PY - 2023 IS - 8 SP - 1396 SN - 2079-4991 DO - 10.3390/nano13081396 UR - https://m2.mtmt.hu/api/publication/33763258 ID - 33763258 AB - In the present work, the chemical synthesis of AgPt nanoalloys is reported by the polyol method using polyvinylpyrrolidone (PVP) as a surfactant and a heterogeneous nucleation approach. Nanoparticles with different atomic compositions of the Ag and Pt elements (1:1 and 1:3) were synthesized by adjusting the molar ratios of the precursors. The physicochemical and microstructural characterization was initially performed using the UV-Vis technique to determine the presence of nanoparticles in suspension. Then, the morphology, size, and atomic structure were determined using XRD, SEM, and HAADF-STEM techniques, confirming the formation of a well-defined crystalline structure and homogeneous nanoalloy with an average particle size of less than 10 nm. Finally, the cyclic voltammetry technique evaluated the electrochemical activity of bimetallic AgPt nanoparticles supported on Vulcan XC-72 carbon for the ethanol oxidation reaction in an alkaline medium. Chronoamperometry and accelerated electrochemical degradation tests were performed to determine their stability and long-term durability. The synthesized AgPt (1:3)/C electrocatalyst presented significative catalytic activity and superior durability due to the introduction of Ag that weakens the chemisorption of the carbonaceous species. Thus, it could be an attractive candidate for cost-effective ethanol oxidation compared to commercial Pt/C. LA - English DB - MTMT ER - TY - JOUR AU - Asma, Syeda Tasmia AU - Imre, Kálmán AU - Morar, Adriana AU - Imre, Mirela AU - Acaroz, Ulas AU - Shah, Syed Rizwan Ali AU - Hussain, Syed Zajif AU - Arslan-Acaroz, Damla AU - Istanbullugil, Fatih Ramazan AU - Madani, Khodir AU - Athanassiou, Christos AU - Atanasoff, Alexander AU - Morar, Doru AU - Herman, Viorel AU - Zhu, Kui TI - Natural Strategies as Potential Weapons against Bacterial Biofilms JF - LIFE-BASEL J2 - LIFE-BASEL VL - 12 PY - 2022 IS - 10 PG - 35 SN - 2075-1729 DO - 10.3390/life12101618 UR - https://m2.mtmt.hu/api/publication/33198734 ID - 33198734 N1 - Funding Agency and Grant Number: project "Increasing the impact of excellence research on the capacity for innovation and technology transfer within USAMVB Timis Funding text: This review is supported by the project "Increasing the impact of excellence research on the capacity for innovation and technology transfer within USAMVB Timis,oara" code 6PFE, submitted in the competition Program 1-development of the national system of research-development, Subprogram 1.2-institutional performance, institutional development projects-development projects of excellence in RDI. AB - Microbial biofilm is an aggregation of microbial species that are either attached to surfaces or organized into an extracellular matrix. Microbes in the form of biofilms are highly resistant to several antimicrobials compared to planktonic microbial cells. Their resistance developing ability is one of the major root causes of antibiotic resistance in health sectors. Therefore, effective antibiofilm compounds are required to treat biofilm-associated health issues. The awareness of biofilm properties, formation, and resistance mechanisms facilitate researchers to design and develop combating strategies. This review highlights biofilm formation, composition, major stability parameters, resistance mechanisms, pathogenicity, combating strategies, and effective biofilm-controlling compounds. The naturally derived products, particularly plants, have demonstrated significant medicinal properties, producing them a practical approach for controlling biofilm-producing microbes. Despite providing effective antibiofilm activities, the plant-derived antimicrobial compounds may face the limitations of less bioavailability and low concentration of bioactive molecules. The microbes-derived and the phytonanotechnology-based antibiofilm compounds are emerging as an effective approach to inhibit and eliminate the biofilm-producing microbes. LA - English DB - MTMT ER - TY - JOUR AU - de Santana Magalhães, Flávia AU - Cardoso, Vicelma Luiz AU - Miranda Reis, Miria Hespanhol TI - Incorporation of silver nanoparticles in a kaolin hollow fiber membrane for efficient removal of Enterobacter cloacae and Escherichia coli from aqueous solutions JF - MATERIALS CHEMISTRY AND PHYSICS J2 - MATER CHEM PHYS VL - 287 PY - 2022 PG - 10 SN - 0254-0584 DO - 10.1016/j.matchemphys.2022.126279 UR - https://m2.mtmt.hu/api/publication/32838557 ID - 32838557 AB - Kaolin hollow fiber membranes were produced by the phase inversion method and silver nanoparticles (AgNPs) were sequentially impregnated in the fiber lumen side. The composite membranes were applied to treat aqueous solutions contaminated with Enterobacter cloacae and Escherichia coli. The prepared pristine kaolin hollow fibers presented an asymmetric pore size distribution. According to mercury intrusion analyses, the sponge like layer presented pore sizes between 1.14 and 2.16 μm, while the micro-voids in the finger like layer presented pore sizes between 7.86 and 9.17 μm. The kaolin hollow fibers presented water permeability of 8.46 ± 0.17 L h−1 m−2·kPa−1 and mechanical resistance of 103.58 ± 14.41 MPa. The AgNPs were properly synthetized and, according to spectroscopic analyses, the AgNP solution presented a single broad peak centered at 400 nm, which indicates a uniform dispersion of the synthetized AgNPs. The synthetized AgNPs presented a spherical and pseudospherical geometry, with average hydrodynamic size of 81.71 ± 8.05 nm, polydispersity index of 0.262 ± 0.012 and zeta potential of −20.7 ± 2.6 mV, which indicate a suitable AgNP stability in the aqueous suspension. The AgNPs were successfully impregnated in the lumen side of kaolin hollow fibers at a concentration of 0.51 ± 0.04 mg cm−1. Energy dispersive spectroscopy images showed that the AgNPs were more concentrated in the hollow fiber inner layer and a homogenous distribution of AgNPs were observed through the fiber cross-section. After AgNP impregnation, the composite hollow fiber membranes presented water permeability of 5.22 ± 0.32 L h−1 m−2 kPa−1. The pristine kaolin membrane enabled a log10 reduction value (LRV) of 2.47 ± 0.05 and 3.72 ± 0.13 for filtrations of aqueous solutions with E. cloacae and E. coli, respectively. For the kaolin hollow fiber membrane impregnated with AgNPs, the LRV value increased to 4.38 ± 0.83 and 4.35 ± 0.25 for filtrations of aqueous solutions with E. cloacae and E. coli, respectively. Thus, the synergetic action of the kaolin membranes impregnated with AgNPs enabled a bacterial reduction greater than 99.9%. The AgNPs decreased the permeate flux through the membrane but, for the filtration of the E. cloacae suspension, mitigated the cake formation on the membrane surface. LA - English DB - MTMT ER - TY - JOUR AU - González-Fernández, Sara AU - Lozano-Iturbe, Victor AU - Menéndez, Mª Fe AU - Ordiales, Helena AU - Fernández-Vega, Iván AU - Merayo, Jesús AU - Vazquez, Fernando AU - Quirós, Luis M. AU - Martín, Carla TI - A Promising Antifungal and Antiamoebic Effect of Silver Nanorings, a Novel Type of AgNP JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 11 PY - 2022 IS - 8 PG - 13 SN - 2079-6382 DO - 10.3390/antibiotics11081054 UR - https://m2.mtmt.hu/api/publication/33049070 ID - 33049070 AB - Silver nanoparticles (AgNPs) play an important role in the medical field due to their potent antimicrobial activity. This, together with the constant emergence of resistance to antimicrobial drugs, means AgNPs are often investigated as an alternative to solve this problem. In this article, we analyzed the antifungal and antiamoebic effects of a recently described type of AgNP, silver nanorings (AgNRs), and compared them with other types of AgNPs. Tests of the activity of AgNPs against various fungal and amoebic species were carried out. In all cases, AgNPs showed a high biocidal effect, although with fungi this depended on the species involved. Antifungal activity was detected by the conditioning of culture media or water but this effect was not dependent on the release of Ag ions. On the other hand, the proliferation of Acanthamoeba castellanii trophozoites was reduced by silver nanorings (AgNRs) and silver nanowires (AgNWs), with AgNWs being capable of totally inhibiting the germination of A. castellanii cysts. AgNRs constitute a new type of AgNP with an antifungal and antiacanthamoebic activity. These results open the door to new and effective antimicrobial therapies as an alternative to the use of antifungals or antiamoebic drugs, thus avoiding the constant appearance of resistance and the difficulty of eradicating infections. LA - English DB - MTMT ER - TY - JOUR AU - Lawal, Sodiq Kolawole AU - Olojede, Samuel Oluwaseun AU - Faborode, Oluwaseun Samuel AU - Aladeyelu, Okikioluwa Stephen AU - Matshipi, Matome Nadab AU - Sulaiman, Sheu Oluwadare AU - Naidu, Edwin Coleridge Stephen AU - Rennie, Carmen Olivia AU - Azu, Onyemaechi Okpara TI - Nanodelivery of antiretroviral drugs to nervous tissues JF - FRONTIERS IN PHARMACOLOGY J2 - FRONT PHARMACOL VL - 13 PY - 2022 SN - 1663-9812 DO - 10.3389/fphar.2022.1025160 UR - https://m2.mtmt.hu/api/publication/33226090 ID - 33226090 AB - Despite the development of effective combined antiretroviral therapy (cART), the neurocognitive impairments associated with human immunodeficiency virus (HIV) remain challenging. The presence of the blood-brain barrier (BBB) and blood-cerebrospinal fluid barrier (BCFB) impedes the adequate penetration of certain antiretroviral drugs into the brain. In addition, reports have shown that some antiretroviral drugs cause neurotoxicity resulting from their interaction with nervous tissues due to long-term systemic exposure. Therefore, the research into the effective therapeutic modality that would cater for the HIV-associated neurocognitive disorders (HAND) and ART toxicity is now receiving broad research attention. Thus, this review explores the latest information in managing HAND using a nanoparticle drug delivery system (NDDS). We discussed the neurotoxicity profile of various approved ART. Also, we explained the applications of silver nanoparticles (AgNPs) in medicine, their different synthesis methods and their interaction with nervous tissues. Lastly, while proposing AgNPs as useful nanoparticles in properly delivering ART to enhance effectiveness and minimize neurocognitive disorders, we hypothesize that the perceived toxicity of AgNPs could be minimized by taking appropriate precautions. One such precaution is using appropriate reducing and stabilizing agents such as trisodium citrate to reduce silver ion Ag + to ground state Ag 0 during the synthesis. Also, the usage of medium-sized, spherical-shaped AgNPs is encouraged in AgNPs-based drug delivery to the brain due to their ability to deliver therapeutic agents across BBB. In addition, characterization and functionalization of the synthesized AgNPs are required during the drug delivery approach. Putting all these factors in place would minimize toxicity and enhance the usage of AgNPs in delivering therapeutic agents across the BBB to the targeted brain tissue and could cater for the HIV-associated neurocognitive disorders and neurotoxic effects of antiretroviral drugs (ARDs). LA - English DB - MTMT ER - TY - JOUR AU - Moustafa, Mahmoud AU - Sayed, Mahmoud AU - Alamri, Saad AU - Alghamdii, Huda AU - Shati, Ali AU - Alrumman, Sulaiman AU - Khatani, Mohmed Al AU - Qthanin, Rahma Al AU - Siddique, Sajda AU - Ahmed, Dalia AU - Negm, Sally AU - Emam, Ahmed Al TI - Synthesis, Characterization and Antifungal Effects of Silver Nanoparticles of Ageratum Conyzoides L. JF - BANGLADESH JOURNAL OF BOTANY J2 - BANGLADESH J BOTANY VL - 51 PY - 2022 IS - 3 SP - 439 EP - 444 PG - 6 SN - 0253-5416 DO - 10.3329/bjb.v51i3.61989 UR - https://m2.mtmt.hu/api/publication/33126590 ID - 33126590 AB - Synthesis and characterization of silver nanoparticles (AgNPs) produced from Ageratum conyzoides leaves extract, as well as their antifungal effectiveness against a variety of plant pathogens were investigated. Absorption peaks at 3674, 3264, 2980, 2940, 2096, 1739, 1545, 1370, 1080 and 700 cm−1 were recorded for the formed nanoparticles using FTIR analysis. According to SEM, XRD and UV spectroscopy results, nanoparticles have spherical and semi- spherical shape, size of 34 to 205 nm with a resonance wavelength at 460 nm. Ageratum conyzoides-AgNPs showed moderate antifungal activity against Penicillium expansum, Macrophomina phaseolina, Alternaria alternata, Drechslera halodes and Fusarium oxysporum f.sp. lycopersici. The fabricated NPs from leaves of A. conyzoides can be applied as antifungal agent.Bangladesh J. Bot. 51(3): 439-444, 2022 (September) LA - English DB - MTMT ER - TY - JOUR AU - Robinson, Janelle R. AU - Isikhuemhen, Omoanghe S. AU - Anike, Felicia N. AU - Subedi, Kiran TI - Physiological Response of Saccharomyces cerevisiae to Silver Stress JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 8 PY - 2022 IS - 5 PG - 12 SN - 2309-608X DO - 10.3390/jof8050539 UR - https://m2.mtmt.hu/api/publication/32838548 ID - 32838548 AB - Silver nanoparticle (AgNP) production and their use as antimicrobial agents is a current area of active research. Biosynthesis is the most sustainable production method, and fungi have become candidates of interest in AgNP production. However, investigations into the physiological responses of fungi due to silver exposure are scanty. This present work utilized two strains of Saccharomyces cerevisiae (one used in commercial fermentation and a naturally occurring strain) to determine the physiological consequences of their transient exposure to AgNO3. The assessments were based on studies involving growth curves, minimal inhibitory concentration assays, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) imaging, and inductively coupled plasma optical emission spectroscopy (ICP-OES). Results indicated (a) the capability of S. cerevisiae to produce silver nanoparticles, even at elevated levels of exposure; (b) strain origin had no significant impact on S. cerevisiae physiological response to AgNO3; and (c) coexposure to copper and silver significantly increased intracellular copper, silver, and calcium in treated yeast cells. In addition, electron microscopy and ICP-OES results revealed that both strains internalized silver after exposure, resulting in the shrunken and distorted physical appearance visible on SEM micrographs of treated cells. Though a promising candidate for AgNPs biosynthesis, this study analyzed the effects of transient silver exposure on S. cerevisiae growth physiology and morphology. LA - English DB - MTMT ER - TY - JOUR AU - Sahli, Célia AU - Moya, Sergio E. AU - Lomas, John S. AU - Gravier-Pelletier, Christine AU - Briandet, Romain AU - Hémadi, Miryana TI - Recent advances in nanotechnology for eradicating bacterial biofilm JF - THERANOSTICS J2 - THERANOSTICS VL - 12 PY - 2022 IS - 5 SP - 2383 EP - 2405 PG - 23 SN - 1838-7640 DO - 10.7150/thno.67296 UR - https://m2.mtmt.hu/api/publication/32729595 ID - 32729595 N1 - Export Date: 3 August 2022 LA - English DB - MTMT ER - TY - JOUR AU - Wijesundera, Samurdhi A. AU - Jayawardana, Kalana W. AU - Yan, Mingdi TI - Trehalose-Modified Silver Nanoparticles as Antibacterial Agents with Reduced Cytotoxicity and Enhanced Uptake by Mycobacteria JF - ACS APPLIED NANO MATERIALS J2 - ACS APPL NANO MATER VL - 5 PY - 2022 IS - 8 SP - 10704 EP - 10714 PG - 11 SN - 2574-0970 DO - 10.1021/acsanm.2c02047 UR - https://m2.mtmt.hu/api/publication/33049124 ID - 33049124 AB - Silver nanoparticles (AgNPs) are potent antimicrobial agents, but their utility is limited due to their relatively high cytotoxicity. In this work, we used trehalose as the ligand to reduce the cytotoxicity of AgNPs without affecting their antimicrobial activities. Trehalose is a disaccharide that is unique to mycobacteria. We showed that trehalose-functionalized AgNPs, AgNP-Tre, drastically increased the viability of A549 cells, especially at high concentrations, for example, from 4% for AgNPs to 67% for AgNP-Tre at 64 μg/mL. The trehalose ligand slowed down the release of silver, and the amount of silver released from AgNP-Tre was less than half of that from AgNPs in the culture medium. Intriguingly, while the maltose (Mal) or tri(ethylene glycol) (TEG) ligand reduced the antibacterial activity of AgNPs againstM. smegmatis (minimal inhibitory concentration (MIC) of AgNP-Mal and AgNP-TEG: 4 μg/mL for 7 nm AgNPs), the activity of AgNP-Tre was similar to that of AgNPs (MIC of AgNP-Tre: 1 μg/mL for 7 nm AgNPs). Uptake experiments revealed that the intracellular concentration of AgNP-Tre was 87 and 114% higher than those of AuNP-Mal and AgNP-TEG, respectively. The increased uptake was attributed to the enhanced interactions of AgNP-Tre with mycobacteria promoted by the trehalose ligand. © 2022 American Chemical Society. LA - English DB - MTMT ER - TY - JOUR AU - Yassin, Mohamed Taha AU - Mostafa, Ashraf Abdel-Fattah AU - Al-Askar, Abdulaziz Abdulrahman AU - Al-Otibi, Fatimah O. TI - Synergistic Antifungal Efficiency of Biogenic Silver Nanoparticles with Itraconazole against Multidrug-Resistant Candidal Strains JF - CRYSTALS J2 - CRYSTALS VL - 12 PY - 2022 IS - 6 PG - 18 SN - 2073-4352 DO - 10.3390/cryst12060816 UR - https://m2.mtmt.hu/api/publication/32872312 ID - 32872312 AB - Fungal infections caused by multidrug-resistant strains are considered one of the leading causes of morbidity and mortality worldwide. Moreover, antifungal medications used in conventional antifungal treatment revealed poor therapeutic effectiveness and possible side effects such as hepatotoxicity, nephrotoxicity, and myelotoxicity. Therefore, the current study was developed to determine the antifungal effectiveness of green synthesized silver nanoparticles (AgNPs) and their synergistic efficiency with antifungal drugs against multidrug-resistant candidal strains. The AgNPs were greenly synthesized using the aqueous peel extract of Punica granatum. In addition, AgNPs were characterized using ultraviolet-visible spectrophotometry (UV/Vis), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), and zeta potential analysis. In this regard, UV-vis analysis indicated SPR of AgNPs at 396 nm, while the particle size distribution revealed that the average particle size was 18.567 ± 1.46 nm. The surface charge of AgNPs was found to be −15.6 mV, indicating their stability in aqueous solutions. The biofabricated AgNPs indicated antifungal activity against Candida tropicalis, C. albicans, and C. glabrata strains showing inhibitory zone diameters of 23.78 ± 0.63, 21.38 ± 0.58, and 16.53 ± 0.21 mm, respectively while their minimum inhibitory concentration (MIC) was found to be 2.5 µg/mL against C. tropicalis strain. AgNPs and itraconazole revealed the highest synergistic activity against the multidrug-resistant strain, C. glabrata, recording a synergism percentage of 74.32%. In conclusion, the biogenic AgNPs in combination with itraconazole drug exhibited potential synergistic activity against different candidal strains indicating their potential usage in the bioformulation of highly effective antifungal agents. LA - English DB - MTMT ER - TY - JOUR AU - Bélteky, Péter AU - Rónavári, Andrea AU - Zakupszky, Dalma AU - Boka, Eszter AU - Igaz, Nóra AU - Szerencsés, Bettina AU - Pfeiffer, Ilona AU - Vágvölgyi, Csaba AU - Csontné Kiricsi, Mónika AU - Kónya, Zoltán TI - Are Smaller Nanoparticles Always Better? Understanding the Biological Effect of Size-Dependent Silver Nanoparticle Aggregation Under Biorelevant Conditions JF - INTERNATIONAL JOURNAL OF NANOMEDICINE J2 - INT J NANOMED VL - 16 PY - 2021 SP - 3021 EP - 3040 PG - 20 SN - 1176-9114 DO - 10.2147/IJN.S304138 UR - https://m2.mtmt.hu/api/publication/31982606 ID - 31982606 N1 - Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Szeged, Hungary Export Date: 31 May 2021 Correspondence Address: Kónya, Z.; Department of Applied and Environmental Chemistry, Rerrich Square 1, Hungary; email: konya@chem.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Joshi, Kanchan M. AU - Shelar, Amruta AU - Kasabe, Umesh AU - Nikam, Latesh K. AU - Pawar, Ramdas A. AU - Sangshetti, Jaiprakash AU - Kale, Bharat B. AU - Singh, Ajay Vikram AU - Patil, Rajendra AU - Chaskar, Manohar G. TI - Biofilm inhibition in Candida albicans with biogenic hierarchical zinc-oxide nanoparticles JF - MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS J2 - MAT SCI ENG C-MATER VL - 134 PY - 2021 PG - 13 SN - 0928-4931 DO - 10.1016/j.msec.2021.112592 UR - https://m2.mtmt.hu/api/publication/32603476 ID - 32603476 AB - The present study demonstrates lignin (L), fragments of lignin (FL), and oxidized fragmented lignin (OFL) as templates for the synthesis of zinc oxide nanoparticles (ZnO NPs) viz., lignin-ZnO (L-ZnO), hierarchical FL-ZnO, and OFL-ZnO NPs. The X-ray diffraction patterns confirmed the formation of phase pure ZnO NPs with a hexagonal wurtzite structure. Electron microscopy confirmed the hierarchical structures with one-dimensional arrays of ZnO NPs with an average particle diameter of 40 nm. The as-synthesized L-ZnO, FL-ZnO, and OFL-ZnO NPs were tested in-vitro for growth and virulence inhibition (morphogenesis and biofilm) in Candida albicans. L-ZnO, FL-ZnO, and OFL-ZnO NPs all inhibited growth and virulence. Growth and virulence inhibitions were highest (more than 90%, respectively at 125, 31.2, and 62.5 μg/mL) in presence of FL-ZnO NPs, indicating that the hierarchical FL-ZnO NPs were potent growth and virulence inhibiting agent than non-hierarchical ZnO NPs. Furthermore, the real-time polymerase chain (RT-PCR) was used to study the virulence inhibition molecular mechanisms of L-ZnO, FL-ZnO, and OFL-ZnO NPs. RT-PCR results showed that the downregulation of phr1, phr2, efg1, hwp1, ras1, als3 and als4, and the upregulation of bcy1, nrg1, and tup1 genes inhibited the virulence in C. albicans. Lastly, we also performed in-vitro test cell cytotoxicity on the cell line, mouse embryo 3T3L1, and in-vivo toxicity on Rats, which showed that FL-ZnO NPs were biocompatible and nontoxic. LA - English DB - MTMT ER - TY - JOUR AU - Mare, Anca Delia AU - Ciurea, Cristina Nicoleta AU - Man, Adrian AU - Mareș, Mihai AU - Toma, Felicia AU - Berța, Lavinia AU - Tanase, Corneliu TI - In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 10 PY - 2021 IS - 10 PG - 15 SN - 2223-7747 DO - 10.3390/plants10102153 UR - https://m2.mtmt.hu/api/publication/32603465 ID - 32603465 AB - Biosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate BBE), their influence on biofilm production, their potential synergistic effects with fluconazole, on different Candida spp., and their influence on virulence factors of C. albicans (germ tube production, gene expression for ALS3, SAP2, HSP70). Both the AgNP BBEs presented different minimum inhibitory concentrations for all the studied Candida spp., but biofilm production was inhibited only for C. albicans and C. guilliermondii. The growth rates of all the studied Candida spp. were inhibited in the presence of both AgNP BBEs, except for C. auris. Synergistic activity was observed for C. parapsilosis and C. guilliermondii, for different combinations of fluconazole with both the AgNP BBEs. The germ tube production of C. albicans was slightly inhibited by the AgNP BBEs. Only AgNP Acetate BBE was able to down-regulate the expression of SAP2. Overall, we can conclude that, even if more studies are necessary, AgNPs synthesized with beech bark extract might be an interesting alternative to classic antifungal treatments. LA - English DB - MTMT ER - TY - JOUR AU - Mendes Junior, Dario AU - Hausen, Moema A. AU - Asami, Jessica AU - Higa, Akemi M. AU - Leite, Fabio L. AU - Mambrini, Giovanni P. AU - Rossi, Andre L. AU - Komatsu, Daniel AU - Duek, Eliana A. de Rezende TI - A New Dermal Substitute Containing Polyvinyl Alcohol with Silver Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 10 PY - 2021 IS - 6 PG - 17 SN - 2079-6382 DO - 10.3390/antibiotics10060742 UR - https://m2.mtmt.hu/api/publication/32242942 ID - 32242942 AB - The experimental use of poly (alcohol-vinyl) (PVA) as a skin curative is increasing widely. However, the use of this hydrogel is challenging due to its favorable properties for microbiota growth. The association with silver nanoparticles (AgNPs) as an antimicrobial agent turns the match for PVA as a dressing, as it focuses on creating a physical barrier to avoid wound dehydration. When associated with extracellular components, such as the collagen matrix, the device obtained can create the desired biological conditions to act as a skin substitute. This study aimed to analyze the anti-microbiological activity and the in vitro and in vivo responses of a bilaminar device of PVA containing AgNPs associated with a membrane of collagen-hyaluronic acid (col-HA). Additionally, mesenchymal stem cells were cultured in the device to evaluate in vitro responses and in vivo immunomodulatory and healing behavior. The device morphology revealed a porous pattern that favored water retention and in vitro cell adhesion. Controlled wounds in the dorsal back of rat skins revealed a striking skin remodeling with new epidermis fulfilling all previously injured areas after 14 and 28 days. No infections or significant inflammations were observed, despite increased angiogenesis, and no fibrosis-markers were identified as compared to controls. Although few antibacterial activities were obtained, the addition of AgNPs prevented fungal growth. All results demonstrated that the combination of the components used here as a dermal device, chosen according to previous miscellany studies of low/mid-cost biomaterials, can promote skin protection avoiding infections and dehydration, minimize the typical wound inflammatory responses, and favor the cellular healing responses, features that give rise to further clinical trials of the device here developed LA - English DB - MTMT ER - TY - JOUR AU - Nami, Sanam AU - Aghebati-Maleki, Ali AU - Aghebati-Maleki, Leili TI - CURRENT APPLICATIONS AND PROSPECTS OF NANOPARTICLES FOR ANTIFUNGAL DRUG DELIVERY JF - EXCLI JOURNAL J2 - EXCLI J VL - 20 PY - 2021 SP - 562 EP - 584 PG - 23 SN - 1611-2156 DO - 10.17179/excli2020-3068 UR - https://m2.mtmt.hu/api/publication/31991630 ID - 31991630 N1 - Funding Agency and Grant Number: Tabriz University of Medical Sciences, Tabriz, Iran Funding text: This work was supported financially by Research Vice-Chancellor, Tabriz University of Medical Sciences, Tabriz, Iran. Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Tabriz University of Medical Sciences, Tabriz, Iran Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Export Date: 28 April 2021 Correspondence Address: Aghebati-Maleki, L.; Immunology, Iran; email: aghebatil@tbzmed.ac.ir AB - Currently, the significance of fungi as human pathogens is not medically concealed in the world. Consequently, suitable recognition and treatment of such infections are of great importance and necessitate the need for comprehensive information in this regard. The introduction of new antifungals and their use today, especially in the last two decades, have revolutionized the treatment of fungal infections. On the other hand, increasing drug resistance in the world has overshadowed such developments. The use of NPs results in the treatment of fungal infections and owing to their specific properties, these particles, unlike the pure antibiotics, can exert a greater inhibitory power although with less concentration compared with conventional drugs. Important reasons that have led to the use of antifungal drugs in delivery systems include reduced drug efficacy, limited penetration through tissue, poor aqueous solubility, decreased bioavailability, and poor drug pharmacokinetics. It is therefore hoped that unfavorable properties of antifungal drugs be mitigated via their incorporation into different types of NPs. This review summarizes the different types of NPs as delivery systems of antifungal as well as their advantages over pure drugs. LA - English DB - MTMT ER - TY - JOUR AU - Ortega, M.P. AU - López-Marín, L.M. AU - Millán-Chiu, B. AU - Manzano-Gayosso, P. AU - Acosta-Torres, L.S. AU - García-Contreras, R. AU - Manisekaran, R. TI - Polymer mediated synthesis of cationic silver nanoparticles as an effective anti-fungal and anti-biofilm agent against Candida species JF - COLLOID AND INTERFACE SCIENCE COMMUNICATIONS J2 - COLLOID INTERFAC SCI VL - 43 PY - 2021 PG - 12 SN - 2215-0382 DO - 10.1016/j.colcom.2021.100449 UR - https://m2.mtmt.hu/api/publication/32107222 ID - 32107222 N1 - Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Campus Juriquilla, Querétaro, Mexico CONACyT - Centro de Fısica Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Juriquilla, Querétaro, Mexico Unidad de Micología, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de, Mexico Laboratorio de Investigación Interdisciplinaria, Área de Nanoestructuras y Biomateriales, Escuela Nacional de Estudios Superiores Unidad León; Universidad Nacional Autónoma de México, León, Guanajuato, Mexico Export Date: 19 July 2021 Correspondence Address: Millán-Chiu, B.; CONACyT - Centro de Fısica Aplicada y Tecnología Avanzada, Mexico; email: blanca.millan@fata.unam.mx AB - “Superbugs”, multidrug-resistant microorganisms, have become a significant threat to the global healthcare sector over the past few decades. Among other diseases, invasive fungal infections caused by the Candida species are the most common cause of nosocomial infections with antifungal resistance. This work has employed a facile aqueous phase process to synthesize a stable, cationic silver nanoparticle using polyethyleneimine (PEI) as both a reducing and stabilizing agent. We determined that the polymer ratio plays a vital role in particle size and charge. As a result, the efficacy of antimicrobial activity was inversely proportional to the particle size from the disc diffusion and microdilution method. Furthermore, the smallest nanoparticles were shown to inhibit biofilm formation of Candida on silicon catheters and proved to be an efficient and potential anticandidal agent against the standard and clinical isolates of Candida. Therefore, the current investigation paves a new avenue for the green fabrication of nano‑silver based anticandidal agents. LA - English DB - MTMT ER - TY - JOUR AU - Rai, Mahendra AU - Ingle, Avinash P. AU - Trzcińska-Wencel, Joanna AU - Wypij, Magdalena AU - Bonde, Shital AU - Yadav, Alka AU - Kratošová, Gabriela AU - Golińska, Patrycja TI - Biogenic Silver Nanoparticles: What We Know and What Do We Need to Know? JF - NANOMATERIALS J2 - NANOMATERIALS-BASEL VL - 11 PY - 2021 IS - 11 PG - 34 SN - 2079-4991 DO - 10.3390/nano11112901 UR - https://m2.mtmt.hu/api/publication/32603470 ID - 32603470 AB - Nanobiotechnology is considered to be one of the fastest emerging fields. It is still a relatively new and exciting area of research with considerable potential for development. Among the inorganic nanomaterials, biogenically synthesized silver nanoparticles (bio-AgNPs) have been frequently used due to their unique physicochemical properties that result not only from their shape and size but also from surface coatings of natural origin. These properties determine antibacterial, antifungal, antiprotozoal, anticancer, anti-inflammatory, and many more activities of bio-AgNPs. This review provides the current state of knowledge on the methods and mechanisms of biogenic synthesis of silver nanoparticles as well as their potential applications in different fields such as medicine, food, agriculture, and industries. LA - English DB - MTMT ER - TY - JOUR AU - Rawaa, SHAKIR SHNAIN AL-SHAMMARI AU - Ahmed, KAREEM HAMMOOD JABERI AU - Ana, LUNGU AU - Ioana, BREBENEL AU - Waleed, KHALED KADDEM AL-SUDANI AU - Jasim, HAFEDH MOHAMMED AL-SAEDI AU - Cristian-Emilian, POP AU - Maria, MERNEA AU - Gheorghe, STOIAN AU - Dan, Florin MIHAILESCU TI - The antifungal activity of zein nanoparticles loaded with transition metal ions JF - REVUE ROUMAINE DE CHIMIE J2 - REV ROUM CHIM VL - 66 PY - 2021 IS - 10-11 SP - 829 EP - 834 PG - 6 SN - 0035-3930 DO - 10.33224/rrch.2021.66.10-11.06 UR - https://m2.mtmt.hu/api/publication/33049216 ID - 33049216 LA - English DB - MTMT ER - TY - JOUR AU - Rónavári, Andrea AU - Igaz, Nóra AU - Adamecz, Dóra Izabella AU - Szerencsés, Bettina AU - Molnár, Csaba AU - Kónya, Zoltán AU - Pfeiffer, Ilona AU - Csontné Kiricsi, Mónika TI - Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications JF - MOLECULES J2 - MOLECULES VL - 26 PY - 2021 IS - 4 PG - 39 SN - 1420-3049 DO - 10.3390/molecules26040844 UR - https://m2.mtmt.hu/api/publication/31852610 ID - 31852610 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office-NKFIHNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.3.2-15-2016-00038, GINOP-2.3.2-15-2016-00035, EFOP-3.6.1-16-201600008]; "Palyazat_kodja_elneve zese""Sorsz" grant of the New National Excellence Program of the Ministry for Innovation and Technology; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [BO/00878/19/8] Funding text: Financial support by the National Research, Development and Innovation Office-NKFIH through projects GINOP-2.3.2-15-2016-00038, GINOP-2.3.2-15-2016-00035 and EFOP-3.6.1-16-201600008 is gratefully acknowledged. Furthermore, this work was supported by a "Palyazat_kodja_elneve zese""Sorsz" (M.K.) grant of the New National Excellence Program of the Ministry for Innovation and Technology and by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/00878/19/8 for M.K.). LA - English DB - MTMT ER - TY - JOUR AU - Rónavári, Andrea AU - Bélteky, Péter AU - Boka, Eszter AU - Zakupszky, Dalma AU - Igaz, Nóra AU - Szerencsés, Bettina AU - Pfeiffer, Ilona AU - Kónya, Zoltán AU - Csontné Kiricsi, Mónika TI - Polyvinyl-Pyrrolidone-Coated Silver Nanoparticles—The Colloidal, Chemical, and Biological Consequences of Steric Stabilization under Biorelevant Conditions JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 16 PG - 21 SN - 1661-6596 DO - 10.3390/ijms22168673 UR - https://m2.mtmt.hu/api/publication/32131688 ID - 32131688 N1 - Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, H-6720, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Szeged, H-6720, Hungary Cited By :2 Export Date: 11 March 2022 Correspondence Address: Kónya, Z.; Department of Applied and Environmental Chemistry, Hungary; email: konya@chem.u-szeged.hu Chemicals/CAS: povidone, 9003-39-8; silver, 7440-22-4; Anti-Infective Agents; Antineoplastic Agents; Povidone; Silver LA - English DB - MTMT ER - TY - JOUR AU - Tazi, Neftaha AU - Pigeon, Xavier AU - Mbuyi-Boisvert, Jérôme Mulamba AU - Giret, Simon AU - Béland, François AU - Rouabhia, Mahmoud TI - Effect of Cannabis Smoke Condensate on C. albicans Growth and Biofilm Formation JF - MICROORGANISMS J2 - MICROORGANISMS VL - 9 PY - 2021 IS - 11 PG - 12 SN - 2076-2607 DO - 10.3390/microorganisms9112348 UR - https://m2.mtmt.hu/api/publication/32603472 ID - 32603472 AB - The most common use of cannabis is smoking. The oral ecosystem, among other constituents, can be deregulated by the presence of cannabis smoke in the oral cavity. We evaluated the effect of cannabis smoke condensate (CSC) on the behavior of Candida albicans, a common yeast found in the oral cavity. The yeast was first cultured with different concentrations of CSC, and its growth was evaluated. The transition from the blastospore to the hyphal form and the hyphae size were assessed after 3 and 6 h, along with biofilm formation after 72 h of contact with CSC. The response of C. albicans to oxidative (H2O2) stress was also examined. Our results show that CSC contained high amounts of THC (about 1055 ppm), CBN (63 ppm), and CBG (about 47 ppm). The presence of various concentrations of CSC in the culture medium increased C. albicans growth. CSC also contributed to increases in both the hyphal length and biofilm mass. Following oxidative stress (H2O2 at either 100 or 500 μM), CSC prevented the damaging effect of H2O2 on both C. albicans shape and growth. These findings support clinical observations demonstrating that cannabis may promote C. albicans growth and oral candidiasis. LA - English DB - MTMT ER -