@article{MTMT:34753137, title = {Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles}, url = {https://m2.mtmt.hu/api/publication/34753137}, author = {Mekky, Alsayed E. and Abdelaziz, Ahmed E. M. and Youssef, Fady Sayed and Elaskary, Shymaa A. and Shoun, Aly A. and Alwaleed, Eman A. and Gaber, Mahmoud Ali and Al-Askar, Abdulaziz A. and Alsamman, Alhadary M. and Yousef, Abdullah and AbdElgayed, Gehad and Suef, Reda A. and Selim, Mohamed A and Saied, Ebrahim and Khedr, Mohamed}, doi = {10.3390/medicina60030515}, journal-iso = {MED LITH}, journal = {MEDICINA-LITHUANIA}, volume = {60}, unique-id = {34753137}, issn = {1010-660X}, abstract = {Background and Objectives: Urinary tract infections [UTIs] are considered the third most known risk of infection in human health around the world. There is increasing appreciation for the pathogenicity of Gram-positive and Gram-negative strains in UTIs, aside from fungal infection, as they have numerous virulence factors. Materials and Methods: In this study, fifty urine samples were collected from patients suffering from UTI. Among the isolates of UTI microbes, six isolates were described as MDR isolates after an antibiotic susceptibility test carried out using ten different antibiotics. An alternative treatment for microbial elimination involved the use of biosynthesized silver nanoparticles (AgNPs) derived from Solanum lycopersicum [S. cumin]. Results: The sizes and shapes of AgNPs were characterized through TEM imaging, which showed spherical particles in a size range of 35–80 nm, of which the average size was 53 nm. Additionally, the silver nanoparticles (AgNPs) demonstrated inhibitory activity against Staphylococcus aureus (OR648079), exhibiting a 31 mm zone of inhibition at a minimum inhibitory concentration (MIC) of 4 mg/mL and a minimum bactericidal concentration (MBC) of 8 mg/mL. This was followed by Aspergillus niger (OR648075), which showed a 30 mm inhibition zone at an MIC of 16 mg/mL and a minimum fungicidal concentration (MFC) of 32 mg/mL. Then, Enterococcus faecalis (OR648078), Klebsiella pneumoniae (OR648081), and Acinetobacter baumannii (OR648080) each displayed a 29 mm zone of inhibition at an MIC of 8 mg/mL and an MBC of 16 mg/mL. The least inhibition was observed against Candida auris (OR648076), with a 25 mm inhibition zone at an MIC of 16 mg/mL and an MFC of 32 mg/mL. Furthermore, AgNPs at different concentrations removed DPPH and H2O2 at an IC50 value of 13.54 μg/mL. Also, AgNPs at 3 mg/mL showed remarkable DNA fragmentation in all bacterial strains except Enterococcus faecalis. The phytochemical analysis showed the presence of different active organic components in the plant extract, which concluded that rutin was 88.3 mg/g, garlic acid was 70.4 mg/g, and tannic acid was 23.7 mg/g. Finally, AgNPs concentrations in the range of 3–6 mg/mL showed decreased expression of two of the fundamental genes necessary for biofilm formation within Staphylococcus aureus, fnbA (6 folds), and Cna (12.5 folds) when compared with the RecA gene, which decreased by one-fold when compared with the control sample. These two genes were submitted with NCBI accession numbers [OR682119] and [OR682118], respectively. Conclusions: The findings from this study indicate that biosynthesized AgNPs from Solanum lycopersicum exhibit promising antimicrobial and antioxidant properties against UTI pathogens, including strains resistant to multiple antibiotics. This suggests their potential as an effective alternative treatment for UTIs. Further research is warranted to fully understand the mechanisms of action and to explore the therapeutic applications of these nanoparticles in combating UTIs.}, year = {2024}, eissn = {1648-9144}, orcid-numbers = {Abdelaziz, Ahmed E. M./0000-0002-1087-3521; Yousef, Abdullah/0000-0002-4519-0034; Suef, Reda A./0000-0001-8080-1104; Selim, Mohamed A/0000-0002-5539-7274; Saied, Ebrahim/0000-0002-5622-7912} } @{MTMT:34549940, title = {Silver-based biofungicides for the suppression of pathogenic fungi in agriculture fields}, url = {https://m2.mtmt.hu/api/publication/34549940}, author = {Seku, Kondaiah and Hussaini, Syed Sulaiman and Bhagavanth Reddy, G. and Radha Krishna Reddy, M.}, booktitle = {Nanofungicides}, doi = {10.1016/B978-0-323-95305-4.00009-1}, unique-id = {34549940}, year = {2024}, pages = {169-194} } @article{MTMT:33924978, title = {Size-tunable Sargassum polycystum mediated synthesis of silver nanoparticles and its larvicidal effect on Aedes aegypti}, url = {https://m2.mtmt.hu/api/publication/33924978}, author = {Lean, Jo Sze and Wan Mohamad Ali, Wan Najdah and Ahmad, Rohani and Mohamed Nor, Zurainee and Wong, Ching Lee and Ng, Jeck Fei}, doi = {10.1007/s10811-023-02997-y}, journal-iso = {J APPL PHYCOL}, journal = {JOURNAL OF APPLIED PHYCOLOGY}, unique-id = {33924978}, issn = {0921-8971}, year = {2023}, eissn = {1573-5176} } @article{MTMT:33681041, title = {Transfer of AgNPs’ Anti-Biofilm Activity into the Nontoxic Polymer Matrix}, url = {https://m2.mtmt.hu/api/publication/33681041}, author = {Mačák, Lívia and Velgosová, Oksana and Múdra, Erika and Vojtko, Marek and Dolinská, Silvia}, doi = {10.3390/polym15051238}, journal-iso = {POLYMERS-BASEL}, journal = {POLYMERS}, volume = {15}, unique-id = {33681041}, abstract = {A biological method was successfully applied to synthesize spherical silver nanoparticles (AgNPs) while using the extract of lavender (Ex-L) (lat. Lavandula angustifolia) as the reducing and stabilizing agent. The produced nanoparticles were spherical with an average size of 20 nm. The AgNPs’ synthesis rate confirmed the extract’s excellent ability to reduce silver nanoparticles from the AgNO3 solution. The presence of good stabilizing agents was confirmed by the excellent stability of the extract. Nanoparticles’ shapes and sizes did not change. UV-Vis absorption spectrometry, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used to characterize the silver nanoparticles. The silver nanoparticles were incorporated into the PVA polymer matrix by the “ex situ” method. The polymer matrix composite with AgNPs was prepared in two ways: as a composite film and nanofibers (nonwoven textile). The anti-biofilm activity of AgNPs and the ability of AgNPs to transfer toxic properties into the polymer matrix were proved.}, year = {2023}, eissn = {2073-4360}, pages = {1238}, orcid-numbers = {Velgosová, Oksana/0000-0001-8903-2604} } @article{MTMT:34179978, title = {Development and evaluation of an anti-candida cream based on silver nanoparticles}, url = {https://m2.mtmt.hu/api/publication/34179978}, author = {Maciel, Antônio Auberson Martins and Cunha, Francisco Afrânio and Freire, Tiago Melo and de Menezes, Fernando Lima and Fechine, Lillian Maria Uchoa Dutra and Rocha, Janaina Sobreira and de Cássia Carvalho Barbosa, Rita and Martins, Roxeane Teles and da Conceição dos Santos Oliveira Cu, Maria and Santos-Oliveira, Ralph and Queiroz, Maria Veraci Oliveira and Fechine, Pierre Basílio Almeida}, doi = {10.1007/s13205-023-03776-9}, journal-iso = {3 BIOTECH}, journal = {3 BIOTECH}, volume = {13}, unique-id = {34179978}, issn = {2190-572X}, year = {2023}, eissn = {2190-5738}, pages = {1}, orcid-numbers = {Fechine, Pierre Basílio Almeida/0000-0002-7822-2354} } @article{MTMT:33763258, title = {Bimetallic AgPt Nanoalloys as an Electrocatalyst for Ethanol Oxidation Reaction: Synthesis, Structural Analysis, and Electro-Catalytic Activity}, url = {https://m2.mtmt.hu/api/publication/33763258}, author = {Mares-Briones, Fabian and Higareda, América and Lopez-Miranda, Jose Luis and Mendoza-Cruz, Rubén and Esparza, Rodrigo}, doi = {10.3390/nano13081396}, journal-iso = {NANOMATERIALS-BASEL}, journal = {NANOMATERIALS}, volume = {13}, unique-id = {33763258}, abstract = {In the present work, the chemical synthesis of AgPt nanoalloys is reported by the polyol method using polyvinylpyrrolidone (PVP) as a surfactant and a heterogeneous nucleation approach. Nanoparticles with different atomic compositions of the Ag and Pt elements (1:1 and 1:3) were synthesized by adjusting the molar ratios of the precursors. The physicochemical and microstructural characterization was initially performed using the UV-Vis technique to determine the presence of nanoparticles in suspension. Then, the morphology, size, and atomic structure were determined using XRD, SEM, and HAADF-STEM techniques, confirming the formation of a well-defined crystalline structure and homogeneous nanoalloy with an average particle size of less than 10 nm. Finally, the cyclic voltammetry technique evaluated the electrochemical activity of bimetallic AgPt nanoparticles supported on Vulcan XC-72 carbon for the ethanol oxidation reaction in an alkaline medium. Chronoamperometry and accelerated electrochemical degradation tests were performed to determine their stability and long-term durability. The synthesized AgPt (1:3)/C electrocatalyst presented significative catalytic activity and superior durability due to the introduction of Ag that weakens the chemisorption of the carbonaceous species. Thus, it could be an attractive candidate for cost-effective ethanol oxidation compared to commercial Pt/C.}, year = {2023}, eissn = {2079-4991}, pages = {1396}, orcid-numbers = {Mendoza-Cruz, Rubén/0000-0002-8831-1983; Esparza, Rodrigo/0000-0001-9743-8559} } @article{MTMT:33198734, title = {Natural Strategies as Potential Weapons against Bacterial Biofilms}, url = {https://m2.mtmt.hu/api/publication/33198734}, author = {Asma, Syeda Tasmia and Imre, Kálmán and Morar, Adriana and Imre, Mirela and Acaroz, Ulas and Shah, Syed Rizwan Ali and Hussain, Syed Zajif and Arslan-Acaroz, Damla and Istanbullugil, Fatih Ramazan and Madani, Khodir and Athanassiou, Christos and Atanasoff, Alexander and Morar, Doru and Herman, Viorel and Zhu, Kui}, doi = {10.3390/life12101618}, journal-iso = {LIFE-BASEL}, journal = {LIFE-BASEL}, volume = {12}, unique-id = {33198734}, abstract = {Microbial biofilm is an aggregation of microbial species that are either attached to surfaces or organized into an extracellular matrix. Microbes in the form of biofilms are highly resistant to several antimicrobials compared to planktonic microbial cells. Their resistance developing ability is one of the major root causes of antibiotic resistance in health sectors. Therefore, effective antibiofilm compounds are required to treat biofilm-associated health issues. The awareness of biofilm properties, formation, and resistance mechanisms facilitate researchers to design and develop combating strategies. This review highlights biofilm formation, composition, major stability parameters, resistance mechanisms, pathogenicity, combating strategies, and effective biofilm-controlling compounds. The naturally derived products, particularly plants, have demonstrated significant medicinal properties, producing them a practical approach for controlling biofilm-producing microbes. Despite providing effective antibiofilm activities, the plant-derived antimicrobial compounds may face the limitations of less bioavailability and low concentration of bioactive molecules. The microbes-derived and the phytonanotechnology-based antibiofilm compounds are emerging as an effective approach to inhibit and eliminate the biofilm-producing microbes.}, year = {2022}, eissn = {2075-1729}, orcid-numbers = {Asma, Syeda Tasmia/0000-0001-5762-1877; Imre, Kálmán/0000-0002-6057-882X; Morar, Adriana/0000-0002-4701-4229; Imre, Mirela/0000-0003-3004-463X; Acaroz, Ulas/0000-0002-1533-4519; Shah, Syed Rizwan Ali/0000-0002-2752-4854; Athanassiou, Christos/0000-0001-6578-4019; Atanasoff, Alexander/0000-0003-3460-9589; Morar, Doru/0000-0002-8629-5983; Herman, Viorel/0000-0001-7118-6488} } @article{MTMT:32838557, title = {Incorporation of silver nanoparticles in a kaolin hollow fiber membrane for efficient removal of Enterobacter cloacae and Escherichia coli from aqueous solutions}, url = {https://m2.mtmt.hu/api/publication/32838557}, author = {de Santana Magalhães, Flávia and Cardoso, Vicelma Luiz and Miranda Reis, Miria Hespanhol}, doi = {10.1016/j.matchemphys.2022.126279}, journal-iso = {MATER CHEM PHYS}, journal = {MATERIALS CHEMISTRY AND PHYSICS}, volume = {287}, unique-id = {32838557}, issn = {0254-0584}, abstract = {Kaolin hollow fiber membranes were produced by the phase inversion method and silver nanoparticles (AgNPs) were sequentially impregnated in the fiber lumen side. The composite membranes were applied to treat aqueous solutions contaminated with Enterobacter cloacae and Escherichia coli. The prepared pristine kaolin hollow fibers presented an asymmetric pore size distribution. According to mercury intrusion analyses, the sponge like layer presented pore sizes between 1.14 and 2.16 μm, while the micro-voids in the finger like layer presented pore sizes between 7.86 and 9.17 μm. The kaolin hollow fibers presented water permeability of 8.46 ± 0.17 L h−1 m−2·kPa−1 and mechanical resistance of 103.58 ± 14.41 MPa. The AgNPs were properly synthetized and, according to spectroscopic analyses, the AgNP solution presented a single broad peak centered at 400 nm, which indicates a uniform dispersion of the synthetized AgNPs. The synthetized AgNPs presented a spherical and pseudospherical geometry, with average hydrodynamic size of 81.71 ± 8.05 nm, polydispersity index of 0.262 ± 0.012 and zeta potential of −20.7 ± 2.6 mV, which indicate a suitable AgNP stability in the aqueous suspension. The AgNPs were successfully impregnated in the lumen side of kaolin hollow fibers at a concentration of 0.51 ± 0.04 mg cm−1. Energy dispersive spectroscopy images showed that the AgNPs were more concentrated in the hollow fiber inner layer and a homogenous distribution of AgNPs were observed through the fiber cross-section. After AgNP impregnation, the composite hollow fiber membranes presented water permeability of 5.22 ± 0.32 L h−1 m−2 kPa−1. The pristine kaolin membrane enabled a log10 reduction value (LRV) of 2.47 ± 0.05 and 3.72 ± 0.13 for filtrations of aqueous solutions with E. cloacae and E. coli, respectively. For the kaolin hollow fiber membrane impregnated with AgNPs, the LRV value increased to 4.38 ± 0.83 and 4.35 ± 0.25 for filtrations of aqueous solutions with E. cloacae and E. coli, respectively. Thus, the synergetic action of the kaolin membranes impregnated with AgNPs enabled a bacterial reduction greater than 99.9%. The AgNPs decreased the permeate flux through the membrane but, for the filtration of the E. cloacae suspension, mitigated the cake formation on the membrane surface.}, year = {2022}, eissn = {1879-3312}, orcid-numbers = {de Santana Magalhães, Flávia/0000-0003-3111-0360} } @article{MTMT:33049070, title = {A Promising Antifungal and Antiamoebic Effect of Silver Nanorings, a Novel Type of AgNP}, url = {https://m2.mtmt.hu/api/publication/33049070}, author = {González-Fernández, Sara and Lozano-Iturbe, Victor and Menéndez, Mª Fe and Ordiales, Helena and Fernández-Vega, Iván and Merayo, Jesús and Vazquez, Fernando and Quirós, Luis M. and Martín, Carla}, doi = {10.3390/antibiotics11081054}, journal-iso = {ANTIBIOTICS-BASEL}, journal = {ANTIBIOTICS}, volume = {11}, unique-id = {33049070}, abstract = {Silver nanoparticles (AgNPs) play an important role in the medical field due to their potent antimicrobial activity. This, together with the constant emergence of resistance to antimicrobial drugs, means AgNPs are often investigated as an alternative to solve this problem. In this article, we analyzed the antifungal and antiamoebic effects of a recently described type of AgNP, silver nanorings (AgNRs), and compared them with other types of AgNPs. Tests of the activity of AgNPs against various fungal and amoebic species were carried out. In all cases, AgNPs showed a high biocidal effect, although with fungi this depended on the species involved. Antifungal activity was detected by the conditioning of culture media or water but this effect was not dependent on the release of Ag ions. On the other hand, the proliferation of Acanthamoeba castellanii trophozoites was reduced by silver nanorings (AgNRs) and silver nanowires (AgNWs), with AgNWs being capable of totally inhibiting the germination of A. castellanii cysts. AgNRs constitute a new type of AgNP with an antifungal and antiacanthamoebic activity. These results open the door to new and effective antimicrobial therapies as an alternative to the use of antifungals or antiamoebic drugs, thus avoiding the constant appearance of resistance and the difficulty of eradicating infections.}, year = {2022}, eissn = {2079-6382}, orcid-numbers = {González-Fernández, Sara/0000-0003-4487-3906; Fernández-Vega, Iván/0000-0002-3141-6493; Quirós, Luis M./0000-0003-3505-3762; Martín, Carla/0000-0001-5353-5461} } @article{MTMT:33226090, title = {Nanodelivery of antiretroviral drugs to nervous tissues}, url = {https://m2.mtmt.hu/api/publication/33226090}, author = {Lawal, Sodiq Kolawole and Olojede, Samuel Oluwaseun and Faborode, Oluwaseun Samuel and Aladeyelu, Okikioluwa Stephen and Matshipi, Matome Nadab and Sulaiman, Sheu Oluwadare and Naidu, Edwin Coleridge Stephen and Rennie, Carmen Olivia and Azu, Onyemaechi Okpara}, doi = {10.3389/fphar.2022.1025160}, journal-iso = {FRONT PHARMACOL}, journal = {FRONTIERS IN PHARMACOLOGY}, volume = {13}, unique-id = {33226090}, abstract = {Despite the development of effective combined antiretroviral therapy (cART), the neurocognitive impairments associated with human immunodeficiency virus (HIV) remain challenging. The presence of the blood-brain barrier (BBB) and blood-cerebrospinal fluid barrier (BCFB) impedes the adequate penetration of certain antiretroviral drugs into the brain. In addition, reports have shown that some antiretroviral drugs cause neurotoxicity resulting from their interaction with nervous tissues due to long-term systemic exposure. Therefore, the research into the effective therapeutic modality that would cater for the HIV-associated neurocognitive disorders (HAND) and ART toxicity is now receiving broad research attention. Thus, this review explores the latest information in managing HAND using a nanoparticle drug delivery system (NDDS). We discussed the neurotoxicity profile of various approved ART. Also, we explained the applications of silver nanoparticles (AgNPs) in medicine, their different synthesis methods and their interaction with nervous tissues. Lastly, while proposing AgNPs as useful nanoparticles in properly delivering ART to enhance effectiveness and minimize neurocognitive disorders, we hypothesize that the perceived toxicity of AgNPs could be minimized by taking appropriate precautions. One such precaution is using appropriate reducing and stabilizing agents such as trisodium citrate to reduce silver ion Ag + to ground state Ag 0 during the synthesis. Also, the usage of medium-sized, spherical-shaped AgNPs is encouraged in AgNPs-based drug delivery to the brain due to their ability to deliver therapeutic agents across BBB. In addition, characterization and functionalization of the synthesized AgNPs are required during the drug delivery approach. Putting all these factors in place would minimize toxicity and enhance the usage of AgNPs in delivering therapeutic agents across the BBB to the targeted brain tissue and could cater for the HIV-associated neurocognitive disorders and neurotoxic effects of antiretroviral drugs (ARDs).}, year = {2022}, eissn = {1663-9812} } @article{MTMT:33126590, title = {Synthesis, Characterization and Antifungal Effects of Silver Nanoparticles of Ageratum Conyzoides L.}, url = {https://m2.mtmt.hu/api/publication/33126590}, author = {Moustafa, Mahmoud and Sayed, Mahmoud and Alamri, Saad and Alghamdii, Huda and Shati, Ali and Alrumman, Sulaiman and Khatani, Mohmed Al and Qthanin, Rahma Al and Siddique, Sajda and Ahmed, Dalia and Negm, Sally and Emam, Ahmed Al}, doi = {10.3329/bjb.v51i3.61989}, journal-iso = {BANGLADESH J BOTANY}, journal = {BANGLADESH JOURNAL OF BOTANY}, volume = {51}, unique-id = {33126590}, issn = {0253-5416}, abstract = {Synthesis and characterization of silver nanoparticles (AgNPs) produced from Ageratum conyzoides leaves extract, as well as their antifungal effectiveness against a variety of plant pathogens were investigated. Absorption peaks at 3674, 3264, 2980, 2940, 2096, 1739, 1545, 1370, 1080 and 700 cm−1 were recorded for the formed nanoparticles using FTIR analysis. According to SEM, XRD and UV spectroscopy results, nanoparticles have spherical and semi- spherical shape, size of 34 to 205 nm with a resonance wavelength at 460 nm. Ageratum conyzoides-AgNPs showed moderate antifungal activity against Penicillium expansum, Macrophomina phaseolina, Alternaria alternata, Drechslera halodes and Fusarium oxysporum f.sp. lycopersici. The fabricated NPs from leaves of A. conyzoides can be applied as antifungal agent.Bangladesh J. Bot. 51(3): 439-444, 2022 (September)}, year = {2022}, eissn = {2079-9926}, pages = {439-444} } @article{MTMT:32838548, title = {Physiological Response of Saccharomyces cerevisiae to Silver Stress}, url = {https://m2.mtmt.hu/api/publication/32838548}, author = {Robinson, Janelle R. and Isikhuemhen, Omoanghe S. and Anike, Felicia N. and Subedi, Kiran}, doi = {10.3390/jof8050539}, journal-iso = {J FUNGI}, journal = {JOURNAL OF FUNGI}, volume = {8}, unique-id = {32838548}, abstract = {Silver nanoparticle (AgNP) production and their use as antimicrobial agents is a current area of active research. Biosynthesis is the most sustainable production method, and fungi have become candidates of interest in AgNP production. However, investigations into the physiological responses of fungi due to silver exposure are scanty. This present work utilized two strains of Saccharomyces cerevisiae (one used in commercial fermentation and a naturally occurring strain) to determine the physiological consequences of their transient exposure to AgNO3. The assessments were based on studies involving growth curves, minimal inhibitory concentration assays, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) imaging, and inductively coupled plasma optical emission spectroscopy (ICP-OES). Results indicated (a) the capability of S. cerevisiae to produce silver nanoparticles, even at elevated levels of exposure; (b) strain origin had no significant impact on S. cerevisiae physiological response to AgNO3; and (c) coexposure to copper and silver significantly increased intracellular copper, silver, and calcium in treated yeast cells. In addition, electron microscopy and ICP-OES results revealed that both strains internalized silver after exposure, resulting in the shrunken and distorted physical appearance visible on SEM micrographs of treated cells. Though a promising candidate for AgNPs biosynthesis, this study analyzed the effects of transient silver exposure on S. cerevisiae growth physiology and morphology.}, year = {2022}, eissn = {2309-608X}, orcid-numbers = {Isikhuemhen, Omoanghe S./0000-0002-7147-6650; Subedi, Kiran/0000-0002-9034-3554} } @article{MTMT:32729595, title = {Recent advances in nanotechnology for eradicating bacterial biofilm}, url = {https://m2.mtmt.hu/api/publication/32729595}, author = {Sahli, Célia and Moya, Sergio E. and Lomas, John S. and Gravier-Pelletier, Christine and Briandet, Romain and Hémadi, Miryana}, doi = {10.7150/thno.67296}, journal-iso = {THERANOSTICS}, journal = {THERANOSTICS}, volume = {12}, unique-id = {32729595}, issn = {1838-7640}, year = {2022}, eissn = {1838-7640}, pages = {2383-2405} } @article{MTMT:33049124, title = {Trehalose-Modified Silver Nanoparticles as Antibacterial Agents with Reduced Cytotoxicity and Enhanced Uptake by Mycobacteria}, url = {https://m2.mtmt.hu/api/publication/33049124}, author = {Wijesundera, Samurdhi A. and Jayawardana, Kalana W. and Yan, Mingdi}, doi = {10.1021/acsanm.2c02047}, journal-iso = {ACS APPL NANO MATER}, journal = {ACS APPLIED NANO MATERIALS}, volume = {5}, unique-id = {33049124}, abstract = {Silver nanoparticles (AgNPs) are potent antimicrobial agents, but their utility is limited due to their relatively high cytotoxicity. In this work, we used trehalose as the ligand to reduce the cytotoxicity of AgNPs without affecting their antimicrobial activities. Trehalose is a disaccharide that is unique to mycobacteria. We showed that trehalose-functionalized AgNPs, AgNP-Tre, drastically increased the viability of A549 cells, especially at high concentrations, for example, from 4% for AgNPs to 67% for AgNP-Tre at 64 μg/mL. The trehalose ligand slowed down the release of silver, and the amount of silver released from AgNP-Tre was less than half of that from AgNPs in the culture medium. Intriguingly, while the maltose (Mal) or tri(ethylene glycol) (TEG) ligand reduced the antibacterial activity of AgNPs againstM. smegmatis (minimal inhibitory concentration (MIC) of AgNP-Mal and AgNP-TEG: 4 μg/mL for 7 nm AgNPs), the activity of AgNP-Tre was similar to that of AgNPs (MIC of AgNP-Tre: 1 μg/mL for 7 nm AgNPs). Uptake experiments revealed that the intracellular concentration of AgNP-Tre was 87 and 114% higher than those of AuNP-Mal and AgNP-TEG, respectively. The increased uptake was attributed to the enhanced interactions of AgNP-Tre with mycobacteria promoted by the trehalose ligand. © 2022 American Chemical Society.}, year = {2022}, eissn = {2574-0970}, pages = {10704-10714}, orcid-numbers = {Yan, Mingdi/0000-0003-1121-4007} } @article{MTMT:32872312, title = {Synergistic Antifungal Efficiency of Biogenic Silver Nanoparticles with Itraconazole against Multidrug-Resistant Candidal Strains}, url = {https://m2.mtmt.hu/api/publication/32872312}, author = {Yassin, Mohamed Taha and Mostafa, Ashraf Abdel-Fattah and Al-Askar, Abdulaziz Abdulrahman and Al-Otibi, Fatimah O.}, doi = {10.3390/cryst12060816}, journal-iso = {CRYSTALS}, journal = {CRYSTALS}, volume = {12}, unique-id = {32872312}, abstract = {Fungal infections caused by multidrug-resistant strains are considered one of the leading causes of morbidity and mortality worldwide. Moreover, antifungal medications used in conventional antifungal treatment revealed poor therapeutic effectiveness and possible side effects such as hepatotoxicity, nephrotoxicity, and myelotoxicity. Therefore, the current study was developed to determine the antifungal effectiveness of green synthesized silver nanoparticles (AgNPs) and their synergistic efficiency with antifungal drugs against multidrug-resistant candidal strains. The AgNPs were greenly synthesized using the aqueous peel extract of Punica granatum. In addition, AgNPs were characterized using ultraviolet-visible spectrophotometry (UV/Vis), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), and zeta potential analysis. In this regard, UV-vis analysis indicated SPR of AgNPs at 396 nm, while the particle size distribution revealed that the average particle size was 18.567 ± 1.46 nm. The surface charge of AgNPs was found to be −15.6 mV, indicating their stability in aqueous solutions. The biofabricated AgNPs indicated antifungal activity against Candida tropicalis, C. albicans, and C. glabrata strains showing inhibitory zone diameters of 23.78 ± 0.63, 21.38 ± 0.58, and 16.53 ± 0.21 mm, respectively while their minimum inhibitory concentration (MIC) was found to be 2.5 µg/mL against C. tropicalis strain. AgNPs and itraconazole revealed the highest synergistic activity against the multidrug-resistant strain, C. glabrata, recording a synergism percentage of 74.32%. In conclusion, the biogenic AgNPs in combination with itraconazole drug exhibited potential synergistic activity against different candidal strains indicating their potential usage in the bioformulation of highly effective antifungal agents.}, year = {2022}, eissn = {2073-4352}, orcid-numbers = {Yassin, Mohamed Taha/0000-0002-0997-8350} } @article{MTMT:31982606, title = {Are Smaller Nanoparticles Always Better? Understanding the Biological Effect of Size-Dependent Silver Nanoparticle Aggregation Under Biorelevant Conditions}, url = {https://m2.mtmt.hu/api/publication/31982606}, author = {Bélteky, Péter and Rónavári, Andrea and Zakupszky, Dalma and Boka, Eszter and Igaz, Nóra and Szerencsés, Bettina and Pfeiffer, Ilona and Vágvölgyi, Csaba and Csontné Kiricsi, Mónika and Kónya, Zoltán}, doi = {10.2147/IJN.S304138}, journal-iso = {INT J NANOMED}, journal = {INTERNATIONAL JOURNAL OF NANOMEDICINE}, volume = {16}, unique-id = {31982606}, issn = {1176-9114}, year = {2021}, eissn = {1178-2013}, pages = {3021-3040}, orcid-numbers = {Rónavári, Andrea/0000-0001-7054-0975; Igaz, Nóra/0000-0003-1580-4397; Pfeiffer, Ilona/0000-0003-0680-7596; Vágvölgyi, Csaba/0000-0003-0009-7773; Csontné Kiricsi, Mónika/0000-0002-8416-2052; Kónya, Zoltán/0000-0002-9406-8596} } @article{MTMT:32603476, title = {Biofilm inhibition in Candida albicans with biogenic hierarchical zinc-oxide nanoparticles}, url = {https://m2.mtmt.hu/api/publication/32603476}, author = {Joshi, Kanchan M. and Shelar, Amruta and Kasabe, Umesh and Nikam, Latesh K. and Pawar, Ramdas A. and Sangshetti, Jaiprakash and Kale, Bharat B. and Singh, Ajay Vikram and Patil, Rajendra and Chaskar, Manohar G.}, doi = {10.1016/j.msec.2021.112592}, journal-iso = {MAT SCI ENG C-MATER}, journal = {MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS}, volume = {134}, unique-id = {32603476}, issn = {0928-4931}, abstract = {The present study demonstrates lignin (L), fragments of lignin (FL), and oxidized fragmented lignin (OFL) as templates for the synthesis of zinc oxide nanoparticles (ZnO NPs) viz., lignin-ZnO (L-ZnO), hierarchical FL-ZnO, and OFL-ZnO NPs. The X-ray diffraction patterns confirmed the formation of phase pure ZnO NPs with a hexagonal wurtzite structure. Electron microscopy confirmed the hierarchical structures with one-dimensional arrays of ZnO NPs with an average particle diameter of 40 nm. The as-synthesized L-ZnO, FL-ZnO, and OFL-ZnO NPs were tested in-vitro for growth and virulence inhibition (morphogenesis and biofilm) in Candida albicans. L-ZnO, FL-ZnO, and OFL-ZnO NPs all inhibited growth and virulence. Growth and virulence inhibitions were highest (more than 90%, respectively at 125, 31.2, and 62.5 μg/mL) in presence of FL-ZnO NPs, indicating that the hierarchical FL-ZnO NPs were potent growth and virulence inhibiting agent than non-hierarchical ZnO NPs. Furthermore, the real-time polymerase chain (RT-PCR) was used to study the virulence inhibition molecular mechanisms of L-ZnO, FL-ZnO, and OFL-ZnO NPs. RT-PCR results showed that the downregulation of phr1, phr2, efg1, hwp1, ras1, als3 and als4, and the upregulation of bcy1, nrg1, and tup1 genes inhibited the virulence in C. albicans. Lastly, we also performed in-vitro test cell cytotoxicity on the cell line, mouse embryo 3T3L1, and in-vivo toxicity on Rats, which showed that FL-ZnO NPs were biocompatible and nontoxic.}, year = {2021}, eissn = {1873-0191} } @article{MTMT:32603465, title = {In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract}, url = {https://m2.mtmt.hu/api/publication/32603465}, author = {Mare, Anca Delia and Ciurea, Cristina Nicoleta and Man, Adrian and Mareș, Mihai and Toma, Felicia and Berța, Lavinia and Tanase, Corneliu}, doi = {10.3390/plants10102153}, journal-iso = {PLANTS-BASEL}, journal = {PLANTS-BASEL}, volume = {10}, unique-id = {32603465}, abstract = {Biosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate BBE), their influence on biofilm production, their potential synergistic effects with fluconazole, on different Candida spp., and their influence on virulence factors of C. albicans (germ tube production, gene expression for ALS3, SAP2, HSP70). Both the AgNP BBEs presented different minimum inhibitory concentrations for all the studied Candida spp., but biofilm production was inhibited only for C. albicans and C. guilliermondii. The growth rates of all the studied Candida spp. were inhibited in the presence of both AgNP BBEs, except for C. auris. Synergistic activity was observed for C. parapsilosis and C. guilliermondii, for different combinations of fluconazole with both the AgNP BBEs. The germ tube production of C. albicans was slightly inhibited by the AgNP BBEs. Only AgNP Acetate BBE was able to down-regulate the expression of SAP2. Overall, we can conclude that, even if more studies are necessary, AgNPs synthesized with beech bark extract might be an interesting alternative to classic antifungal treatments.}, year = {2021}, eissn = {2223-7747}, orcid-numbers = {Ciurea, Cristina Nicoleta/0000-0003-2262-7456; Man, Adrian/0000-0001-5281-0601; Mareș, Mihai/0000-0002-3103-4954; Berța, Lavinia/0000-0002-2563-8946; Tanase, Corneliu/0000-0002-5900-743X} } @article{MTMT:32242942, title = {A New Dermal Substitute Containing Polyvinyl Alcohol with Silver Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches}, url = {https://m2.mtmt.hu/api/publication/32242942}, author = {Mendes Junior, Dario and Hausen, Moema A. and Asami, Jessica and Higa, Akemi M. and Leite, Fabio L. and Mambrini, Giovanni P. and Rossi, Andre L. and Komatsu, Daniel and Duek, Eliana A. de Rezende}, doi = {10.3390/antibiotics10060742}, journal-iso = {ANTIBIOTICS-BASEL}, journal = {ANTIBIOTICS}, volume = {10}, unique-id = {32242942}, abstract = {The experimental use of poly (alcohol-vinyl) (PVA) as a skin curative is increasing widely. However, the use of this hydrogel is challenging due to its favorable properties for microbiota growth. The association with silver nanoparticles (AgNPs) as an antimicrobial agent turns the match for PVA as a dressing, as it focuses on creating a physical barrier to avoid wound dehydration. When associated with extracellular components, such as the collagen matrix, the device obtained can create the desired biological conditions to act as a skin substitute. This study aimed to analyze the anti-microbiological activity and the in vitro and in vivo responses of a bilaminar device of PVA containing AgNPs associated with a membrane of collagen-hyaluronic acid (col-HA). Additionally, mesenchymal stem cells were cultured in the device to evaluate in vitro responses and in vivo immunomodulatory and healing behavior. The device morphology revealed a porous pattern that favored water retention and in vitro cell adhesion. Controlled wounds in the dorsal back of rat skins revealed a striking skin remodeling with new epidermis fulfilling all previously injured areas after 14 and 28 days. No infections or significant inflammations were observed, despite increased angiogenesis, and no fibrosis-markers were identified as compared to controls. Although few antibacterial activities were obtained, the addition of AgNPs prevented fungal growth. All results demonstrated that the combination of the components used here as a dermal device, chosen according to previous miscellany studies of low/mid-cost biomaterials, can promote skin protection avoiding infections and dehydration, minimize the typical wound inflammatory responses, and favor the cellular healing responses, features that give rise to further clinical trials of the device here developed}, keywords = {Wound healing; Polyvinyl Alcohol; Silver nanoparticles; dermal substitute}, year = {2021}, eissn = {2079-6382}, orcid-numbers = {Asami, Jessica/0000-0002-6171-9305; Komatsu, Daniel/0000-0002-2441-8650} } @article{MTMT:31991630, title = {CURRENT APPLICATIONS AND PROSPECTS OF NANOPARTICLES FOR ANTIFUNGAL DRUG DELIVERY}, url = {https://m2.mtmt.hu/api/publication/31991630}, author = {Nami, Sanam and Aghebati-Maleki, Ali and Aghebati-Maleki, Leili}, doi = {10.17179/excli2020-3068}, journal-iso = {EXCLI J}, journal = {EXCLI JOURNAL}, volume = {20}, unique-id = {31991630}, issn = {1611-2156}, abstract = {Currently, the significance of fungi as human pathogens is not medically concealed in the world. Consequently, suitable recognition and treatment of such infections are of great importance and necessitate the need for comprehensive information in this regard. The introduction of new antifungals and their use today, especially in the last two decades, have revolutionized the treatment of fungal infections. On the other hand, increasing drug resistance in the world has overshadowed such developments. The use of NPs results in the treatment of fungal infections and owing to their specific properties, these particles, unlike the pure antibiotics, can exert a greater inhibitory power although with less concentration compared with conventional drugs. Important reasons that have led to the use of antifungal drugs in delivery systems include reduced drug efficacy, limited penetration through tissue, poor aqueous solubility, decreased bioavailability, and poor drug pharmacokinetics. It is therefore hoped that unfavorable properties of antifungal drugs be mitigated via their incorporation into different types of NPs. This review summarizes the different types of NPs as delivery systems of antifungal as well as their advantages over pure drugs.}, keywords = {IN-VITRO; NANOPARTICLES; Drug delivery; AMPHOTERICIN-B; magnetic nanoparticles; pulmonary delivery; SOLID LIPID NANOPARTICLES; Polymeric nanoparticles; Topical delivery; controlled-release formulation; LOADED NANOPARTICLES}, year = {2021}, eissn = {1611-2156}, pages = {562-584} } @article{MTMT:32107222, title = {Polymer mediated synthesis of cationic silver nanoparticles as an effective anti-fungal and anti-biofilm agent against Candida species}, url = {https://m2.mtmt.hu/api/publication/32107222}, author = {Ortega, M.P. and López-Marín, L.M. and Millán-Chiu, B. and Manzano-Gayosso, P. and Acosta-Torres, L.S. and García-Contreras, R. and Manisekaran, R.}, doi = {10.1016/j.colcom.2021.100449}, journal-iso = {COLLOID INTERFAC SCI}, journal = {COLLOID AND INTERFACE SCIENCE COMMUNICATIONS}, volume = {43}, unique-id = {32107222}, issn = {2215-0382}, abstract = {“Superbugs”, multidrug-resistant microorganisms, have become a significant threat to the global healthcare sector over the past few decades. Among other diseases, invasive fungal infections caused by the Candida species are the most common cause of nosocomial infections with antifungal resistance. This work has employed a facile aqueous phase process to synthesize a stable, cationic silver nanoparticle using polyethyleneimine (PEI) as both a reducing and stabilizing agent. We determined that the polymer ratio plays a vital role in particle size and charge. As a result, the efficacy of antimicrobial activity was inversely proportional to the particle size from the disc diffusion and microdilution method. Furthermore, the smallest nanoparticles were shown to inhibit biofilm formation of Candida on silicon catheters and proved to be an efficient and potential anticandidal agent against the standard and clinical isolates of Candida. Therefore, the current investigation paves a new avenue for the green fabrication of nano‑silver based anticandidal agents.}, year = {2021}, eissn = {2215-0382} } @article{MTMT:32603470, title = {Biogenic Silver Nanoparticles: What We Know and What Do We Need to Know?}, url = {https://m2.mtmt.hu/api/publication/32603470}, author = {Rai, Mahendra and Ingle, Avinash P. and Trzcińska-Wencel, Joanna and Wypij, Magdalena and Bonde, Shital and Yadav, Alka and Kratošová, Gabriela and Golińska, Patrycja}, doi = {10.3390/nano11112901}, journal-iso = {NANOMATERIALS-BASEL}, journal = {NANOMATERIALS}, volume = {11}, unique-id = {32603470}, abstract = {Nanobiotechnology is considered to be one of the fastest emerging fields. It is still a relatively new and exciting area of research with considerable potential for development. Among the inorganic nanomaterials, biogenically synthesized silver nanoparticles (bio-AgNPs) have been frequently used due to their unique physicochemical properties that result not only from their shape and size but also from surface coatings of natural origin. These properties determine antibacterial, antifungal, antiprotozoal, anticancer, anti-inflammatory, and many more activities of bio-AgNPs. This review provides the current state of knowledge on the methods and mechanisms of biogenic synthesis of silver nanoparticles as well as their potential applications in different fields such as medicine, food, agriculture, and industries.}, year = {2021}, eissn = {2079-4991}, orcid-numbers = {Rai, Mahendra/0000-0003-0291-0422; Trzcińska-Wencel, Joanna/0000-0001-9119-0054; Kratošová, Gabriela/0000-0001-6585-8735; Golińska, Patrycja/0000-0001-9154-8191} } @article{MTMT:33049216, title = {The antifungal activity of zein nanoparticles loaded with transition metal ions}, url = {https://m2.mtmt.hu/api/publication/33049216}, author = {Rawaa, SHAKIR SHNAIN AL-SHAMMARI and Ahmed, KAREEM HAMMOOD JABERI and Ana, LUNGU and Ioana, BREBENEL and Waleed, KHALED KADDEM AL-SUDANI and Jasim, HAFEDH MOHAMMED AL-SAEDI and Cristian-Emilian, POP and Maria, MERNEA and Gheorghe, STOIAN and Dan, Florin MIHAILESCU}, doi = {10.33224/rrch.2021.66.10-11.06}, journal-iso = {REV ROUM CHIM}, journal = {REVUE ROUMAINE DE CHIMIE}, volume = {66}, unique-id = {33049216}, issn = {0035-3930}, year = {2021}, pages = {829-834} } @article{MTMT:31852610, title = {Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications}, url = {https://m2.mtmt.hu/api/publication/31852610}, author = {Rónavári, Andrea and Igaz, Nóra and Adamecz, Dóra Izabella and Szerencsés, Bettina and Molnár, Csaba and Kónya, Zoltán and Pfeiffer, Ilona and Csontné Kiricsi, Mónika}, doi = {10.3390/molecules26040844}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {26}, unique-id = {31852610}, issn = {1420-3049}, year = {2021}, eissn = {1420-3049}, orcid-numbers = {Rónavári, Andrea/0000-0001-7054-0975; Igaz, Nóra/0000-0003-1580-4397; Adamecz, Dóra Izabella/0000-0002-1883-9600; Molnár, Csaba/0000-0002-6124-1209; Kónya, Zoltán/0000-0002-9406-8596; Pfeiffer, Ilona/0000-0003-0680-7596; Csontné Kiricsi, Mónika/0000-0002-8416-2052} } @article{MTMT:32131688, title = {Polyvinyl-Pyrrolidone-Coated Silver Nanoparticles—The Colloidal, Chemical, and Biological Consequences of Steric Stabilization under Biorelevant Conditions}, url = {https://m2.mtmt.hu/api/publication/32131688}, author = {Rónavári, Andrea and Bélteky, Péter and Boka, Eszter and Zakupszky, Dalma and Igaz, Nóra and Szerencsés, Bettina and Pfeiffer, Ilona and Kónya, Zoltán and Csontné Kiricsi, Mónika}, doi = {10.3390/ijms22168673}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {22}, unique-id = {32131688}, issn = {1661-6596}, year = {2021}, eissn = {1422-0067}, orcid-numbers = {Rónavári, Andrea/0000-0001-7054-0975; Igaz, Nóra/0000-0003-1580-4397; Pfeiffer, Ilona/0000-0003-0680-7596; Kónya, Zoltán/0000-0002-9406-8596; Csontné Kiricsi, Mónika/0000-0002-8416-2052} } @article{MTMT:32603472, title = {Effect of Cannabis Smoke Condensate on C. albicans Growth and Biofilm Formation}, url = {https://m2.mtmt.hu/api/publication/32603472}, author = {Tazi, Neftaha and Pigeon, Xavier and Mbuyi-Boisvert, Jérôme Mulamba and Giret, Simon and Béland, François and Rouabhia, Mahmoud}, doi = {10.3390/microorganisms9112348}, journal-iso = {MICROORGANISMS}, journal = {MICROORGANISMS}, volume = {9}, unique-id = {32603472}, issn = {2076-2607}, abstract = {The most common use of cannabis is smoking. The oral ecosystem, among other constituents, can be deregulated by the presence of cannabis smoke in the oral cavity. We evaluated the effect of cannabis smoke condensate (CSC) on the behavior of Candida albicans, a common yeast found in the oral cavity. The yeast was first cultured with different concentrations of CSC, and its growth was evaluated. The transition from the blastospore to the hyphal form and the hyphae size were assessed after 3 and 6 h, along with biofilm formation after 72 h of contact with CSC. The response of C. albicans to oxidative (H2O2) stress was also examined. Our results show that CSC contained high amounts of THC (about 1055 ppm), CBN (63 ppm), and CBG (about 47 ppm). The presence of various concentrations of CSC in the culture medium increased C. albicans growth. CSC also contributed to increases in both the hyphal length and biofilm mass. Following oxidative stress (H2O2 at either 100 or 500 μM), CSC prevented the damaging effect of H2O2 on both C. albicans shape and growth. These findings support clinical observations demonstrating that cannabis may promote C. albicans growth and oral candidiasis.}, year = {2021}, eissn = {2076-2607}, orcid-numbers = {Rouabhia, Mahmoud/0000-0003-2205-8281} }