TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Magyar, Lilla Brigitta AU - Kovács, Henrietta AU - Varga, Viktória AU - Bodai, László AU - Zsindely, Nóra AU - Nagy, Gábor AU - Hegedűs, Zoltán AU - Hultmark, Dan AU - Andó, István TI - Distinctive features of Zaprionus indianus hemocyte differentiation and function revealed by transcriptomic analysis JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2023.1322381 UR - https://m2.mtmt.hu/api/publication/34446740 ID - 34446740 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Dolezal, Tomas TI - How to eliminate pathogen without killing oneself? Immunometabolism of encapsulation and melanization in Drosophila JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 PG - 7 SN - 1664-3224 DO - 10.3389/fimmu.2023.1330312 UR - https://m2.mtmt.hu/api/publication/34597768 ID - 34597768 AB - Cellular encapsulation associated with melanization is a crucial component of the immune response in insects, particularly against larger pathogens. The infection of a Drosophila larva by parasitoid wasps, like Leptopilina boulardi, is the most extensively studied example. In this case, the encapsulation and melanization of the parasitoid embryo is linked to the activation of plasmatocytes that attach to the surface of the parasitoid. Additionally, the differentiation of lamellocytes that encapsulate the parasitoid, along with crystal cells, is accountable for the melanization process. Encapsulation and melanization lead to the production of toxic molecules that are concentrated in the capsule around the parasitoid and, at the same time, protect the host from this toxic immune response. Thus, cellular encapsulation and melanization represent primarily a metabolic process involving the metabolism of immune cell activation and differentiation, the production of toxic radicals, but also the production of melanin and antioxidants. As such, it has significant implications for host physiology and systemic metabolism. Proper regulation of metabolism within immune cells, as well as at the level of the entire organism, is therefore essential for an efficient immune response and also impacts the health and overall fitness of the organism that survives. The purpose of this "perspective" article is to map what we know about the metabolism of this type of immune response, place it in the context of possible implications for host physiology, and highlight open questions related to the metabolism of this important insect immune response. LA - English DB - MTMT ER - TY - JOUR AU - Hirschhaeuser, Alexander AU - Molitor, Darius AU - Salinas, Gabriela AU - Grosshans, Jorg AU - Rust, Katja AU - Bogdan, Sven TI - Single-cell transcriptomics identifies new blood cell populations in Drosophila released at the onset of metamorphosis JF - DEVELOPMENT J2 - DEVELOPMENT VL - 150 PY - 2023 IS - 18 PG - 17 SN - 0950-1991 DO - 10.1242/dev.201767 UR - https://m2.mtmt.hu/api/publication/34298309 ID - 34298309 N1 - Institute of Physiology and Pathophysiology, Department of Molecular Cell Physiology, Philipps University Marburg, Emil-Mannkopff-Strasse 2, Marburg, 35037, Germany NGS-Integrative Genomics Core Unit, Department of Human Genetics, University Medical Center Göttingen, Justus von Liebig Weg 11, Göttingen, 37077, Germany Department of Biology, Philipps University Marburg, Karlvon-Frisch-Strasse 8, Marburg, 35043, Germany Cited By :2 Export Date: 13 February 2024 CODEN: DEVPE Correspondence Address: Rust, K.; Institute of Physiology and Pathophysiology, Emil-Mannkopff-Strasse 2, Germany; email: rustka@staff.uni-marburg.de Correspondence Address: Bogdan, S.; Institute of Physiology and Pathophysiology, Emil-Mannkopff-Strasse 2, Germany; email: sven.bogdan@staff.uni-marburg.de AB - Drosophila blood cells called hemocytes form an efficient barrier against infections and tissue damage. During metamorphosis, hemocytes undergo tremendous changes in their shape and behavior, preparing them for tissue clearance. Yet, the diversity and functional plasticity of pupal blood cells have not been explored. Here, we combine single-cell transcriptomics and high-resolution microscopy to dissect the heterogeneity and plasticity of pupal hemocytes. We identified undifferentiated and specified hemocytes with different molecular signatures associated with distinct functions such as antimicrobial, antifungal immune defense, cell adhesion or secretion. Strikingly, we identified a highly migratory and immuneresponsive pupal cell population expressing typical markers of the posterior signaling center (PSC), which is known to be an important niche in the larval lymph gland. PSC-like cells become restricted to the abdominal segments and are morphologically very distinct from experiments further suggest that PSC-like cells can transdifferentiate to lamellocytes triggered by parasitoid wasp infestation. In summary, we present the first molecular description of pupal Drosophila blood cells, providing insights into blood cell functional diversification and plasticity during pupal metamorphosis. LA - English DB - MTMT ER - TY - JOUR AU - Khalili, Dilan AU - Mohammed, Mubasher AU - Kunc, Martin AU - Sindlerova, Martina AU - Ankarklev, Johan AU - Theopold, Ulrich TI - Single-cell sequencing of tumor-associated macrophages in a Drosophila model JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 SN - 1664-3224 DO - 10.3389/fimmu.2023.1243797 UR - https://m2.mtmt.hu/api/publication/34162188 ID - 34162188 LA - English DB - MTMT ER - TY - JOUR AU - Kúthy-Sutus, Enikő AU - Kharrat, Bayan AU - Gábor, Erika AU - Csordás, Gábor AU - Sinka, Rita AU - Honti, Viktor TI - A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 1 PG - 15 SN - 2073-4409 DO - 10.3390/cells12010024 UR - https://m2.mtmt.hu/api/publication/33555087 ID - 33555087 N1 - Export Date: 24 January 2023 AB - The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo. LA - English DB - MTMT ER - TY - JOUR AU - Meyer, C. AU - Drechsler, M. AU - Meyer, H. AU - Paululat, A. TI - Differentiation and function of cardiac valves in the adult Drosophila heart JF - JOURNAL OF EXPERIMENTAL BIOLOGY J2 - J EXP BIOL VL - 226 PY - 2023 IS - 13 SN - 0022-0949 DO - 10.1242/jeb.245839 UR - https://m2.mtmt.hu/api/publication/34110633 ID - 34110633 N1 - Department of Biology/Chemistry, Zoology & Developmental Biology, Osnabrück University, Osnabrück, 49076, Germany Center of Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, 49076, Germany Export Date: 25 August 2023 CODEN: JEBIA Correspondence Address: Paululat, A.; Department of Biology/Chemistry, Germany; email: achim.paululat@uos.de AB - Drosophila, like all insects, has an open circulatory system for the distribution of haemolymph and its components. The circulation of the haemolymph is essentially driven by the pumping activity of the linear heart. The heart is constructed as a tube into which the haemolymph is sucked and pumped forward by rhythmic contractions running from the posterior to the anterior, where it leaves the heart tube. The heart harbours cardiac valves to regulate flow directionality, with a single heart valve differentiating during larval development to separate the heart tube into two chambers. During metamorphosis, the heart is partially restructured, with the linear heart tube with one terminal wide-lumen heart chamber being converted into a linear four-chambered heart tube with three valves. As in all metazoan circulatory systems, the cardiac valves play an essential role in regulating the direction of blood flow. We provide evidence that the valves in adult flies arise via transdifferentiation, converting lumen-forming contractile cardiomyocytes into differently structured valve cells. Interestingly, adult cardiac valves exhibit a similar morphology to their larval counterparts, but act differently upon heart beating. Applying calcium imaging in living specimens to analyse activity in valve cells, we show that adult cardiac valves operate owing to muscle contraction. However, valve cell shape dynamics are altered compared with larval valves, which led us to propose our current model of the opening and closing mechanisms in the fly heart. © 2023. Published by The Company of Biologists Ltd. LA - English DB - MTMT ER - TY - JOUR AU - Quicray, M. AU - Wilhelm, L. AU - Enriquez, T. AU - He, S. AU - Scheifler, M. AU - Visser, B. TI - The Drosophila-parasitizing wasp Leptopilina heterotoma: A comprehensive model system in ecology and evolution JF - ECOLOGY AND EVOLUTION J2 - ECOL EVOL VL - 13 PY - 2023 IS - 1 SN - 2045-7758 DO - 10.1002/ece3.9625 UR - https://m2.mtmt.hu/api/publication/33628955 ID - 33628955 N1 - Export Date: 8 February 2023 AB - The parasitoid Leptopilina heterotoma has been used as a model system for more than 70 years, contributing greatly to diverse research areas in ecology and evolution. Here, we synthesized the large body of work on L. heterotoma with the aim to identify new research avenues that could be of interest also for researchers studying other parasitoids and insects. We start our review with a description of typical L. heterotoma characteristics, as well as that of the higher taxonomic groups to which this species belongs. We then continue discussing host suitability and immunity, foraging behaviors, as well as fat accumulation and life histories. We subsequently shift our focus towards parasitoid-parasitoid interactions, including L. heterotoma coexistence within the larger guild of Drosophila parasitoids, chemical communication, as well as mating and population structuring. We conclude our review by highlighting the assets of L. heterotoma as a model system, including its intermediate life history syndromes, the ease of observing and collecting natural hosts and wasps, as well as recent genomic advances. © 2023 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. LA - English DB - MTMT ER - TY - JOUR AU - Sheng, Y. AU - Chen, J. AU - Jiang, H. AU - Lu, Y. AU - Dong, Z. AU - Pang, L. AU - Zhang, J. AU - Wang, Y. AU - Chen, X. AU - Huang, J. TI - The vitellogenin receptor gene contributes to mating and host-searching behaviors in parasitoid wasps JF - ISCIENCE J2 - ISCIENCE VL - 26 PY - 2023 IS - 4 SN - 2589-0042 DO - 10.1016/j.isci.2023.106298 UR - https://m2.mtmt.hu/api/publication/33809732 ID - 33809732 N1 - Export Date: 11 May 2023 AB - Vitellogenin receptor (VgR) is essential to vitellogenin uptaking and dominates ovary maturation in insects. However, the function of VgR in parasitoid wasps is largely unknown. Here, we applied the Drosophila parasitoid Leptopilina boulardi as a study model to investigate the function of VgR in parasitoids. Despite the conserved sequence characteristics with other insect VgRs, we found L. boulardi VgR (LbVgR) gene was highly expressed in head but lower in ovary. In addition, we found that LbVgR had no effects on ovary development, but participated in host-searching behavior of female L. boulardi and mating behavior of male L. boulardi. Comparative transcriptome analysis further revealed LbVgR might play crucial roles in regulating the expression of some important chemoreception genes to adjust the parasitoid behaviors. These results will broaden our knowledge of the function of VgR in insects, and contribute to develop advanced pest management strategies using parasitoids as biocontrol agents. © 2023 The Author(s) LA - English DB - MTMT ER - TY - JOUR AU - Wu, Zhiwei AU - Yuan, Ruizhong AU - Gu, Qijuan AU - Wu, Xiaotong AU - Gu, Licheng AU - Ye, Xiqian AU - Zhou, Yuenan AU - Huang, Jianhua AU - Wang, Zhizhi AU - Chen, Xuexin TI - Parasitoid Serpins Evolve Novel Functions to Manipulate Host Homeostasis JF - MOLECULAR BIOLOGY AND EVOLUTION J2 - MOL BIOL EVOL VL - 40 PY - 2023 IS - 12 PG - 21 SN - 0737-4038 DO - 10.1093/molbev/msad269 UR - https://m2.mtmt.hu/api/publication/34530178 ID - 34530178 N1 - Funding Agency and Grant Number: Laboratory of Lingnan Modern Agriculture Project [NT2021003]; Natural Science Foundation of China [U22A20485, 32272607]; Key Research and Development Program of Zhejiang Province [2021C02045] Funding text: This research was funded by the Laboratory of Lingnan Modern Agriculture Project (NT2021003), the Natural Science Foundation of China (U22A20485 and 32272607), and the Key Research and Development Program of Zhejiang Province (2021C02045) AB - Parasitoids introduce various virulence factors when parasitism occurs, and some taxa generate teratocytes to manipulate the host immune system and metabolic homeostasis for the survival and development of their progeny. Host-parasitoid interactions are extremely diverse and complex, yet the evolutionary dynamics are still poorly understood. A category of serpin genes, named CvT-serpins, was discovered to be specifically expressed and secreted by the teratocytes of Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella. Genomic and phylogenetic analysis indicated that the C. vestalis serpin genes are duplicated and most of them are clustered into 1 monophyletic clade. Intense positive selection was detected at the residues around the P1-P1 ' cleavage sites of the Cv-serpin reactive center loop domain. Functional analyses revealed that, in addition to the conserved function of melanization inhibition (CvT-serpins 1, 16, 18, and 21), CvT-serpins exhibited novel functions, i.e. bacteriostasis (CvT-serpins 3 and 5) and nutrient metabolism regulation (CvT-serpins 8 and 10). When the host-parasitoid system is challenged with foreign bacteria, CvT-serpins act as an immune regulator to reprogram the host immune system through sustained inhibition of host melanization while simultaneously functioning as immune effectors to compensate for this suppression. In addition, we provided evidence that CvT-serpin8 and 10 participate in the regulation of host trehalose and lipid levels by affecting genes involved in these metabolic pathways. These findings illustrate an exquisite tactic by which parasitoids win out in the parasite-host evolutionary arms race by manipulating host immune and nutrition homeostasis via adaptive gene evolution and neofunctionalization. LA - English DB - MTMT ER - TY - JOUR AU - Bakopoulos, D. AU - Whisstock, J.C. AU - Warr, C.G. AU - Johnson, T.K. TI - Macrophage self-renewal is regulated by transient expression of PDGF- and VEGF-related factor 2 JF - FEBS JOURNAL J2 - FEBS J VL - 289 PY - 2022 IS - 13 SP - 3735 EP - 3751 PG - 17 SN - 1742-464X DO - 10.1111/febs.16364 UR - https://m2.mtmt.hu/api/publication/32876827 ID - 32876827 N1 - Export Date: 14 June 2022 CODEN: FJEOA AB - Macrophages are an ancient blood cell lineage critical for homeostasis and defence against pathogens. Although their numbers were long thought to be sustained solely by haematopoietic organs, it has recently become clear that their proliferation, or self-renewal, also plays a major role. In the Drosophila larva, macrophages undergo a phase of rapid self-renewal, making this an attractive model for elucidating the signals and regulatory mechanisms involved. However, a central self-renewal pathway has not been identified in this system. Here, we show that the PDGF- and VEGF-receptor related (Pvr) pathway fulfils this role. Our data show that two of the three known Pvr ligands, PDGF- and VEGF-related factor 2 (Pvf2) and Pvf3, are major determinants of overall macrophage numbers, yet they each act in a temporally independent manner and via distinct mechanisms. While Pvf3 is needed prior to the self-renewal period, we find that Pvf2 is critical specifically for expanding the larval macrophage population. We further show that Pvf2 is a potent macrophage mitogen that is kept at limiting quantities by its transient expression in a remarkably small number of blood cells. Together, these data support a novel mechanism for the regulation of macrophage self-renewal rates by the dynamic transcriptional control of Pvf2. Given the strong parallels that exist between Drosophila and vertebrate macrophage systems, it is likely that a similar self-renewal control mechanism is at play across animal phyla. © 2022 Federation of European Biochemical Societies LA - English DB - MTMT ER - TY - JOUR AU - Bland, M.L. TI - Regulating metabolism to shape immune function: Lessons from Drosophila JF - SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY J2 - SEMIN CELL DEV BIOL PY - 2022 SN - 1084-9521 DO - 10.1016/j.semcdb.2022.04.002 UR - https://m2.mtmt.hu/api/publication/32915215 ID - 32915215 N1 - Export Date: 1 July 2022 CODEN: SCDBF AB - Infection with pathogenic microbes is a severe threat that hosts manage by activating the innate immune response. In Drosophila melanogaster, the Toll and Imd signaling pathways are activated by pathogen-associated molecular patterns to initiate cellular and humoral immune processes that neutralize and kill invaders. The Toll and Imd signaling pathways operate in organs such as fat body and gut that control host nutrient metabolism, and infections or genetic activation of Toll and Imd signaling also induce wide-ranging changes in host lipid, carbohydrate and protein metabolism. Metabolic regulation by immune signaling can confer resistance to or tolerance of infection, but it can also lead to pathology and susceptibility to infection. These immunometabolic phenotypes are described in this review, as are changes in endocrine signaling and gene regulation that mediate survival during infection. Future work in the field is anticipated to determine key variables such as sex, dietary nutrients, life stage, and pathogen characteristics that modify immunometabolic phenotypes and, importantly, to uncover the mechanisms used by the immune system to regulate metabolism. © 2022 LA - English DB - MTMT ER - TY - JOUR AU - Dai, M.-L. AU - Ye, W.-T. AU - Jiang, X.-J. AU - Feng, P. AU - Zhu, Q.-Y. AU - Sun, H.-N. AU - Li, F.-C. AU - Wei, J. AU - Li, B. TI - Effect of Tachinid Parasitoid Exorista japonica on the Larval Development and Pupation of the Host Silkworm Bombyx mori JF - FRONTIERS IN PHYSIOLOGY J2 - FRONT PHYSIOL VL - 13 PY - 2022 SN - 1664-042X DO - 10.3389/fphys.2022.824203 UR - https://m2.mtmt.hu/api/publication/32915214 ID - 32915214 N1 - Export Date: 1 July 2022 AB - The Tachinidae are natural enemies of many lepidopteran and coleopteran pests of crops, forests, and fruits. However, host-tachinid parasitoid interactions have been largely unexplored. In this study, we investigated the effects of tachinids on host biological traits, using Exorista japonica, a generalist parasitoid, and the silkworm Bombyx mori, its lepidopteran host, as models. We observed that E. japonica parasitoidism did not affect silkworm larval body weight gain and cocooning rate, whereas they caused shortened duration of molting from the final instar to the pupal stage, abnormal molting from larval to pupal stages, and a subsequent decrease in host emergence rate. Moreover, a decrease in juvenile hormone (JH) titer and an increase in 20-hydroxyecdysone (20E) titer in the hemolymph of parasitized silkworms occurred. The transcription of JH and 20E responsive genes was downregulated in mature parasitized hosts, but upregulated in parasitized prepupae while Fushi tarazu factor 1 (Ftz-f1), a nuclear receptor essential in larval ecdysis, showed dramatically reduced expression in parasitized hosts at both the mature and prepupal stages. Moreover, the transcriptional levels of BmFtz-f1 and its downstream target genes encoding cuticle proteins were downregulated in epidermis of parasitized hosts. Meanwhile, the content of trehalose was decreased in the hemolymph, while chitin content in the epidermis was increased in parasitized silkworm prepupae. These data reveal that the host may fine-tune JH and 20E synthesis to shorten developmental duration to combat established E. japonica infestation, while E. japonica silences BmFtz-f1 transcription to inhibit host pupation. This discovery highlights the novel target mechanism of tachinid parasitoids and provides new clues to host/tachinid parasitoid relationships. Copyright © 2022 Dai, Ye, Jiang, Feng, Zhu, Sun, Li, Wei and Li. LA - English DB - MTMT ER - TY - JOUR AU - Evans, C.J. AU - Liu, T. AU - Girard, J.R. AU - Banerjee, U. TI - Injury-induced inflammatory signaling and hematopoiesis in Drosophila JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 119 PY - 2022 IS - 12 SN - 0027-8424 DO - 10.1073/pnas.2119109119 UR - https://m2.mtmt.hu/api/publication/32915213 ID - 32915213 N1 - Export Date: 1 July 2022 CODEN: PNASA AB - Inflammatory response in Drosophila to sterile (axenic) injury in embryos and adults has received some attention in recent years, and most concentrate on the events at the injury site. Here we focus on the effect sterile injury has on the hematopoietic organ, the lymph gland, and the circulating blood cells in the larva, the developmental stage at which major events of hematopoiesis are evident. In mammals, injury activates Toll-like receptor/NF-κB signaling in macrophages, which then express and secrete secondary, proinflammatory cytokines. In Drosophila larvae, distal puncture injury of the body wall epidermis causes a rapid activation of Toll and Jun kinase (JNK) signaling throughout the hematopoietic system and the differentiation of a unique blood cell type, the lamellocyte. Furthermore, we find that Toll and JNK signaling are coupled in their activation. Secondary to this Toll/JNK response, a cytokine, Upd3, is induced as a Toll pathway transcriptional target, which then promotes JAK/STAT signaling within the blood cells. Toll and JAK/STAT signaling are required for the emergence of the injury-induced lamellocytes. This is akin to the derivation of specialized macrophages in mammalian systems. Upstream, at the injury site, a Duox-@@@@@and peroxide-dependent signal causes the activation of the proteases Grass and SPE, needed for the activation of the Toll-ligand Spz, but microbial sensors or the proteases most closely associated with them during septic injury are not involved in the axenic inflammatory response. Copyright © 2022 the Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Eychenne, Magali AU - Girard, Pierre-Alain AU - Frayssinet, Marie AU - Lan, Laijiao AU - Pages, Sylvie AU - Duvic, Bernard AU - Negre, Nicolas TI - Mutagenesis of both prophenoloxidases in the fall armyworm induces major defects in metamorphosis JF - JOURNAL OF INSECT PHYSIOLOGY J2 - J INSECT PHYSIOL VL - 139 PY - 2022 SP - 4399 EP - 4399 PG - 9 SN - 0022-1910 UR - https://m2.mtmt.hu/api/publication/33318026 ID - 33318026 AB - Upon infection, the phenoloxidase system in arthropods is rapidly mobilized and constitutes a major defense system against invaders. The activation of the key enzymes prophenoloxidase (PPO) and their action in immunity through melanization and encapsulation of foreign bodies in hemolymph has been described in many insects. On the other hand, little is known about PPOs involvement in other essential functions related to insect development. In this paper, we investigated the function of the two PPOs of the crop pest, Spodoptera frugiperda (PPO1 and PPO2). We show that PPOs are mainly expressed in hemocytes with the PPO2 expressed at higher levels than the PPO1. In addition, these two genes are expressed in the same tissue and at the same stages of insect development. Through the generation of loss-of-function mutants by CRISPR/Cas9 method, we show that the presence of PPOs is essential for the normal development of the pupa and the survival of the insect. LA - English DB - MTMT ER - TY - JOUR AU - Gomez-Alonso, Itzia AU - Baltierra-Uribe, Shantal AU - Sanchez-Torres, Luvia AU - Cancino-Diaz, Mario AU - Cancino-Diaz, Juan AU - Rodriguez-Martinez, Sandra AU - Ovruski, Sergio M. AU - Hendrichs, Jorge AU - Cancino, Jorge TI - Irradiation and parasitism affect the ability of larval hemocytes of Anastrepha obliqua for phagocytosis and the production of reactive oxygen species JF - ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY J2 - ARCH INSECT BIOCHEM PY - 2022 PG - 19 SN - 0739-4462 DO - 10.1002/arch.21953 UR - https://m2.mtmt.hu/api/publication/33178825 ID - 33178825 AB - The development of the parasitoid Doryctobracon crawfordi (Viereck) (Hymenoptera: Braconidae) in Anastrepha obliqua (McQuart) (Diptera: Tephritidae) larvae is unviable in nature; however, if the host larva is irradiated at 160 Gy, the parasitoid develops and emerges successfully. This suggests that radiation affects the immune responses of A. obliqua larvae, while the underlying mechanisms remain to be revealed. Using optical and electronic microscopies we determined the number and type of hemocyte populations found inside the A. obliqua larvae, either nonirradiated, irradiated at 160 Gy, parasitized by D. crawfordi, or irradiated and parasitized. Based on flow cytometry, the capacity to produce reactive oxygen species (ROS) was determined by the 123-dihydrorhodamine method in those hemocyte cells. Five cell populations were found in the hemolymph of A. obliqua larvae, two of which (granulocytes and plasmatocytes) can phagocytize and produce ROS. A reduction in the number of cells, mainly of the phagocytic type, was observed, as well as the capacity of these cells to produce ROS, when A. obliqua larvae were irradiated. Both radiation and parasitization decreased the ROS production, and when A. obliqua larvae were irradiated followed by parasitization by D. crawfordi, the reduction of the ROS level was even greater. In contrast, a slight increase in the size of these cells was observed in the hemolymph of the parasitized larvae compared to those in nonparasitized larvae. These results suggest that radiation significantly affects the phagocytic cells of A. obliqua and thus permits the development of the parasitoid D. crawfordi. LA - English DB - MTMT ER - TY - JOUR AU - Hultmark, Dan AU - Andó, István TI - Hematopoietic plasticity mapped in Drosophila and other insects JF - ELIFE J2 - ELIFE VL - 11 PY - 2022 PG - 36 SN - 2050-084X DO - 10.7554/eLife.78906 UR - https://m2.mtmt.hu/api/publication/33039275 ID - 33039275 N1 - Funding Agency and Grant Number: Vetenskapsradet [2018-05114]; Hungarian Science Foundation [K135877] Funding text: Vetenskapsradet 2018-05114 Dan HultmarkHungarian Science Foundation K135877 Istvan AndoThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. LA - English DB - MTMT ER - TY - JOUR AU - Kharrat, Bayan AU - Csordás, Gábor AU - Honti, Viktor TI - Peeling Back the Layers of Lymph Gland Structure and Regulation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 14 PG - 16 SN - 1661-6596 DO - 10.3390/ijms23147767 UR - https://m2.mtmt.hu/api/publication/33050458 ID - 33050458 N1 - Cited By :2 Export Date: 24 January 2023 AB - During the past 60 years, the fruit fly, Drosophila melanogaster, has proven to be an excellent model to study the regulation of hematopoiesis. This is not only due to the evolutionarily conserved signalling pathways and transcription factors contributing to blood cell fate, but also to convergent evolution that led to functional similarities in distinct species. An example of convergence is the compartmentalization of blood cells, which ensures the quiescence of hematopoietic stem cells and allows for the rapid reaction of the immune system upon challenges. The lymph gland, a widely studied hematopoietic organ of the Drosophila larva, represents a microenvironment with similar features and functions to classical hematopoietic stem cell niches of vertebrates. Lymph gland studies were effectively supported by the unparalleled toolkit developed in Drosophila, which enabled the high-resolution investigation of the cellular composition and regulatory interaction networks of the lymph gland. In this review, we summarize how our understanding of lymph gland structure and hematopoietic cell-to-cell communication evolved during the past decades and compare their analogous features to those of the vertebrate hematopoietic stem cell niche. LA - English DB - MTMT ER - TY - JOUR AU - Smith, Madison AU - Hammond, Luke AU - Grueber, Wesley AU - Shin, Grace TI - NEURON-MACROPHAGE INTERACTIONS IN MODELS OF CHEMOTHERAPY-INDUCED PERIPHERAL NEUROPATHY JF - JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM J2 - J PERIPHER NERV SYST VL - 27 PY - 2022 SP - S123 EP - S124 PG - 2 SN - 1085-9489 UR - https://m2.mtmt.hu/api/publication/33318025 ID - 33318025 LA - English DB - MTMT ER - TY - JOUR AU - Wertheim, B. TI - Adaptations and counter-adaptations in Drosophila host–parasitoid interactions: advances in the molecular mechanisms JF - CURRENT OPINION IN INSECT SCIENCE J2 - CURR OPIN INSECT SCI VL - 51 PY - 2022 SN - 2214-5745 DO - 10.1016/j.cois.2022.100896 UR - https://m2.mtmt.hu/api/publication/32876692 ID - 32876692 N1 - Export Date: 14 June 2022 AB - Both hosts and parasitoids evolved a diverse array of traits and strategies for their antagonistic interactions, affecting their chances of encounter, attack and survival after parasitoid attack. This review summarizes the recent progress that has been made in elucidating the molecular mechanisms of these adaptations and counter-adaptations in various Drosophila host–parasitoid interactions. For the hosts, it focuses on the neurobiological and genetic control of strategies in Drosophila adults and larvae of avoidance or escape behaviours upon sensing the parasitoids, and the immunological defences involving diverse classes of haemocytes. For the parasitoids, it highlights their behavioural strategies in host finding, as well as the rich variety of venom components that evolved and were partially acquired through horizontal gene transfer. Recent studies revealed the mechanisms by which these venom components manipulate their parasitized hosts in exhibiting escape behaviour to avoid superparasitism, and their counter-strategies to evade or obstruct the hosts’ immunological defences. © 2022 The Author(s) LA - English DB - MTMT ER - TY - JOUR AU - Wu, Xiaotong AU - Wu, Zhiwei AU - Ye, Xiqian AU - Pang, Lan AU - Sheng, Yifeng AU - Wang, Zehua AU - Zhou, Yuenan AU - Zhu, Jiachen AU - Hu, Rongmin AU - Zhou, Sicong AU - Chen, Jiani AU - Wang, Zhizhi AU - Shi, Min AU - Huang, Jianhua AU - Chen, Xuexin TI - The Dual Functions of a Bracovirus C-Type Lectin in Caterpillar Immune Response Manipulation JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 13 PY - 2022 PG - 16 SN - 1664-3224 DO - 10.3389/fimmu.2022.877027 UR - https://m2.mtmt.hu/api/publication/32972124 ID - 32972124 N1 - Funding Agency and Grant Number: Key Project of Laboratory of Lingnan Modern Agriculture [NT2021003]; Key Program of National Natural Science Foundation of China [31630060]; National Key Research and Development Program of China [2019YFD0300104]; National Science Fund for Excellent Young Scholars [31622048]; National Science Foundation of China [32172467, 31672079]; Zhejiang Provincial Natural Science Foundation [LR18C140001] Funding text: This work was jointly supported by the Key Project of Laboratory of Lingnan Modern Agriculture (NT2021003), Key Program of National Natural Science Foundation of China (31630060), National Key Research and Development Program of China (2019YFD0300104) to XC, the National Science Fund for Excellent Young Scholars (31622048), and the National Science Foundation of China (32172467) to JH, and the National Science Foundation of China (31672079), and Zhejiang Provincial Natural Science Foundation (LR18C140001) to MS. AB - Parasitoids are widespread in natural ecosystems and normally equipped with diverse viral factors to defeat host immune responses. On the other hand, parasitoids can enhance the antibacterial abilities and improve the hypoimmunity traits of parasitized hosts that may encounter pathogenic infections. These adaptive strategies guarantee the survival of parasitoid offspring, yet their underlying mechanisms are poorly understood. Here, we focused on Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella, and found that C. vestalis parasitization decreases the number of host hemocytes, leading to disruption of the encapsulation reaction. We further found that one bracovirus C-type lectin gene, CvBV_28-1, is highly expressed in the hemocytes of parasitized hosts and participates in suppressing the proliferation rate of host hemocytes, which in turn reduces their population and represses the process of encapsulation. Moreover, CvBV_28-1 presents a classical bacterial clearance ability via the agglutination response in a Ca2+-dependent manner in response to gram-positive bacteria. Our study provides insights into the innovative strategy of a parasitoid-derived viral gene that has dual functions to manipulate host immunity for a successful parasitism. LA - English DB - MTMT ER - TY - JOUR AU - Yu, S. AU - Luo, F. AU - Xu, Y. AU - Zhang, Y. AU - Jin, L.H. TI - Drosophila Innate Immunity Involves Multiple Signaling Pathways and Coordinated Communication Between Different Tissues JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 13 PY - 2022 PG - 23 SN - 1664-3224 DO - 10.3389/fimmu.2022.905370 UR - https://m2.mtmt.hu/api/publication/33041863 ID - 33041863 N1 - Export Date: 8 August 2022 AB - The innate immune response provides the first line of defense against invading pathogens, and immune disorders cause a variety of diseases. The fruit fly Drosophila melanogaster employs multiple innate immune reactions to resist infection. First, epithelial tissues function as physical barriers to prevent pathogen invasion. In addition, macrophage-like plasmatocytes eliminate intruders through phagocytosis, and lamellocytes encapsulate large particles, such as wasp eggs, that cannot be phagocytosed. Regarding humoral immune responses, the fat body, equivalent to the mammalian liver, secretes antimicrobial peptides into hemolymph, killing bacteria and fungi. Drosophila has been shown to be a powerful in vivo model for studying the mechanism of innate immunity and host-pathogen interactions because Drosophila and higher organisms share conserved signaling pathways and factors. Moreover, the ease with which Drosophila genetic and physiological characteristics can be manipulated prevents interference by adaptive immunity. In this review, we discuss the signaling pathways activated in Drosophila innate immunity, namely, the Toll, Imd, JNK, JAK/STAT pathways, and other factors, as well as relevant regulatory networks. We also review the mechanisms by which different tissues, including hemocytes, the fat body, the lymph gland, muscles, the gut and the brain coordinate innate immune responses. Furthermore, the latest studies in this field are outlined in this review. In summary, understanding the mechanism underlying innate immunity orchestration in Drosophila will help us better study human innate immunity-related diseases. Copyright © 2022 Yu, Luo, Xu, Zhang and Jin. LA - English DB - MTMT ER - TY - JOUR AU - Zhou, Sicong AU - Lu, Yueqi AU - Chen, Jiani AU - Pan, Zhongqiu AU - Pang, Lan AU - Wang, Ying AU - Zhang, Qichao AU - Strand, Michael R. AU - Chen, Xue-Xin AU - Huang, Jianhua TI - Parasite reliance on its host gut microbiota for nutrition and survival JF - ISME JOURNAL J2 - ISME J VL - 16 PY - 2022 IS - 11 SP - 2574 EP - 2586 PG - 13 SN - 1751-7362 DO - 10.1038/s41396-022-01301-z UR - https://m2.mtmt.hu/api/publication/33318023 ID - 33318023 N1 - Cited By :3 Export Date: 11 May 2023 AB - Studying the microbial symbionts of eukaryotic hosts has revealed a range of interactions that benefit host biology. Most eukaryotes are also infected by parasites that adversely affect host biology for their own benefit. However, it is largely unclear whether the ability of parasites to develop in hosts also depends on host-associated symbionts, e.g., the gut microbiota. Here, we studied the parasitic wasp Leptopilina boulardi (Lb) and its host Drosophila melanogaster. Results showed that Lb successfully develops in conventional hosts (CN) with a gut microbiota but fails to develop in axenic hosts (AX) without a gut microbiota. We determined that developing Lb larvae consume fat body cells that store lipids. We also determined that much larger amounts of lipid accumulate in fat body cells of parasitized CN hosts than parasitized AX hosts. CN hosts parasitized by Lb exhibited large increases in the abundance of the bacterium Acetobacter pomorum in the gut, but did not affect the abundance of Lactobacillus fructivorans which is another common member of the host gut microbiota. However, AX hosts inoculated with A. pomorum and/or L. fructivorans did not rescue development of Lb. In contrast, AX larvae inoculated with A. pomorum plus other identified gut community members including a Bacillus sp. substantially rescued Lb development. Rescue was further associated with increased lipid accumulation in host fat body cells. Insulin-like peptides increased in brain neurosecretory cells of parasitized CN larvae. Lipid accumulation in the fat body of CN hosts was further associated with reduced Bmm lipase activity mediated by insulin/insulin-like growth factor signaling (IIS). Altogether, our results identify a previously unknown role for the gut microbiota in defining host permissiveness for a parasite. Our findings also identify a new paradigm for parasite manipulation of host metabolism that depends on insulin signaling and the gut microbiota. LA - English DB - MTMT ER - TY - JOUR AU - Balog, József Ágoston AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Kari, Beáta AU - Puskás, László AU - Andó, István AU - Szebeni, Gábor TI - Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry JF - GENOMICS PROTEOMICS & BIOINFORMATICS J2 - GENOM PROTEOM BIOINF VL - 19 PY - 2021 IS - 2 SP - 243 EP - 252 PG - 10 SN - 1672-0229 DO - 10.1016/j.gpb.2020.06.022 UR - https://m2.mtmt.hu/api/publication/31940120 ID - 31940120 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030, GINOP-2.3.2-15-2016-00035, NKFI NN118207, NKFI K120142, NKFI 120140, OTKA K-131484]; New National Excellence Program of the Ministry for Innovation and Technology, Hungary [UNKP-19-4-SZTE-36]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [BO/00139/17/8] Funding text: This work was supported by the grants from the National Research, Development and Innovation Office, Hungary (Grant Nos. GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030 to LGP, GINOP-2.3.2-15-2016-00035 to E ' K, NKFI NN118207 and NKFI K120142 to IA, NKFI 120140 to E ' K, and OTKA K-131484 to VH). Ga ' bor J. Szebeni was supported by the New National Excellence Program of the Ministry for Innovation and Technology, Hungary (Grant No. UNKP-19-4-SZTE-36) and by the Ja ' nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (Grant No. BO/00139/17/8). We are grateful to Mrs. Olga Kovalcsik for the technical help. LA - English DB - MTMT ER - TY - JOUR AU - Chen, Jiani AU - Fang, Gangqi AU - Pang, Lan AU - Sheng, Yifeng AU - Zhang, Qichao AU - Zhou, Yuenan AU - Zhou, Sicong AU - Lu, Yueqi AU - Liu, Zhiguo AU - Zhang, Yixiang AU - Li, Guiyun AU - Shi, Min AU - Chen, Xuexin AU - Zhan, Shuai AU - Huang, Jianhua TI - Neofunctionalization of an ancient domain allows parasites to avoid intraspecific competition by manipulating host behaviour JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 12 PY - 2021 IS - 1 PG - 15 SN - 2041-1723 DO - 10.1038/s41467-021-25727-9 UR - https://m2.mtmt.hu/api/publication/32426748 ID - 32426748 N1 - Institute of Insect Sciences, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Zhejiang University, Hangzhou, China State Key Lab of Rice Biology, Zhejiang University, Hangzhou, China Export Date: 19 January 2022 Correspondence Address: Chen, X.; Institute of Insect Sciences, China; email: xxchen@zju.edu.cn Correspondence Address: Huang, J.; Institute of Insect Sciences, China; email: jhhuang@zju.edu.cn Correspondence Address: Zhan, S.; CAS Key Laboratory of Insect Developmental and Evolutionary Biology, China; email: szhan@sibs.ac.cn AB - Intraspecific competition is a major force in mediating population dynamics, fuelling adaptation, and potentially leading to evolutionary diversification. Among the evolutionary arms races between parasites, one of the most fundamental and intriguing behavioural adaptations and counter-adaptations are superparasitism and superparasitism avoidance. However, the underlying mechanisms and ecological contexts of these phenomena remain underexplored. Here, we apply the Drosophila parasite Leptopilina boulardi as a study system and find that this solitary endoparasitic wasp provokes a host escape response for superparasitism avoidance. We combine multi-omics and in vivo functional studies to characterize a small set of RhoGAP domain-containing genes that mediate the parasite's manipulation of host escape behaviour by inducing reactive oxygen species in the host central nervous system. We further uncover an evolutionary scenario in which neofunctionalization and specialization gave rise to the novel role of RhoGAP domain in avoiding superparasitism, with an ancestral origin prior to the divergence between Leptopilina specialist and generalist species. Our study suggests that superparasitism avoidance is adaptive for a parasite and adds to our understanding of how the molecular manipulation of host behaviour has evolved in this system.Evolutionary arms races can drive adaptations in hosts and parasites as well as among competing parasites. A combination of multi-omics and functional tests identifies a set of genes that allow a parasitic wasp to minimize intraspecific competition by inducing hosts to escape before more wasps can parasitize them. LA - English DB - MTMT ER - TY - JOUR AU - Coates, Jonathon Alexis AU - Brooks, Elliot AU - Brittle, Amy Louise AU - Armitage, Emma Louise AU - Zeidler, Martin Peter AU - Evans, Iwan Robert TI - Identification of functionally distinct macrophage subpopulations in Drosophila JF - ELIFE J2 - ELIFE VL - 10 PY - 2021 PG - 39 SN - 2050-084X DO - 10.7554/eLife.58686 UR - https://m2.mtmt.hu/api/publication/32361808 ID - 32361808 N1 - Department of Biomedical Science and the Bateson Centre, University of Sheffield, Sheffield, United Kingdom Department of Infection, Immunity and Cardiovascular Disease and the Bateson Centre, University of Sheffield, Sheffield, United Kingdom Cited By :1 Export Date: 19 January 2022 Correspondence Address: Evans, I.R.; Department of Infection, United Kingdom; email: i.r.evans@sheffield.ac.uk AB - Vertebrate macrophages are a highly heterogeneous cell population, but while Drosophila blood is dominated by a macrophage-like lineage (plasmatocytes), until very recently these cells were considered to represent a homogeneous population. Here, we present our identification of enhancer elements labelling plasmatocyte subpopulations, which vary in abundance across development. These subpopulations exhibit functional differences compared to the overall population, including more potent injury responses and differential localisation and dynamics in pupae and adults. Our enhancer analysis identified candidate genes regulating plasmatocyte behaviour: pan-plasmatocyte expression of one such gene (Calnexin14D) improves wound responses, causing the overall population to resemble more closely the subpopulation marked by the Calnexin14D-associated enhancer. Finally, we show that exposure to increased levels of apoptotic cell death modulates subpopulation cell numbers. Taken together this demonstrates macrophage heterogeneity in Drosophila, identifies mechanisms involved in subpopulation specification and function and facilitates the use of Drosophila to study macrophage heterogeneity in vivo. LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Gábor, Erika AU - Honti, Viktor TI - There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 469 PY - 2021 SP - 135 EP - 143 PG - 9 SN - 0012-1606 DO - 10.1016/j.ydbio.2020.10.006 UR - https://m2.mtmt.hu/api/publication/31743832 ID - 31743832 N1 - Cited By :5 Export Date: 14 June 2022 CODEN: DEBIA LA - English DB - MTMT ER - TY - JOUR AU - Eleftherianos, Ioannis AU - Heryanto, Christa AU - Bassal, Taha AU - Zhang, Wei AU - Tettamanti, Gianluca AU - Mohamed, Amr TI - Haemocyte-mediated immunity in insects: Cells, processes and associated components in the fight against pathogens and parasites JF - IMMUNOLOGY J2 - IMMUNOLOGY VL - 164 PY - 2021 IS - 3 SP - 401 EP - 432 PG - 32 SN - 0019-2805 DO - 10.1111/imm.13390 UR - https://m2.mtmt.hu/api/publication/32361576 ID - 32361576 N1 - Infection and Innate Immunity Laboratory, Department of Biological Sciences, Institute for Biomedical Sciences, The George Washington University, Washington, DC, United States Department of Entomology, Faculty of Science, Cairo University, Giza, Egypt State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Napoli Federico II, Napoli, Italy Cited By :4 Export Date: 19 January 2022 CODEN: IMMUA Correspondence Address: Eleftherianos, I.; Infection and Innate Immunity Laboratory, United States; email: ioannise@gwu.edu Correspondence Address: Mohamed, A.; Department of Entomology, Egypt; email: mamr@sci.cu.edu.eg AB - The host defence of insects includes a combination of cellular and humoral responses. The cellular arm of the insect innate immune system includes mechanisms that are directly mediated by haemocytes (e.g., phagocytosis, nodulation and encapsulation). In addition, melanization accompanying coagulation, clot formation and wound healing, nodulation and encapsulation processes leads to the formation of cytotoxic redox-cycling melanin precursors and reactive oxygen and nitrogen species. However, demarcation between cellular and humoral immune reactions as two distinct categories is not straightforward. This is because many humoral factors affect haemocyte functions and haemocytes themselves are an important source of many humoral molecules. There is also a considerable overlap between cellular and humoral immune functions that span from recognition of foreign intruders to clot formation. Here, we review these immune reactions starting with the cellular mechanisms that limit haemolymph loss and participate in wound healing and clot formation and advancing to cellular functions that are critical in restricting pathogen movement and replication. This information is important because it highlights that insect cellular immunity is controlled by a multilayered system, different components of which are activated by different pathogens or during the different stages of the infection. LA - English DB - MTMT ER - TY - JOUR AU - Gautam, D.K. AU - Chimata, A.V. AU - Gutti, R.K. AU - Paddibhatla, I. TI - Comparative hematopoiesis and signal transduction in model organisms JF - JOURNAL OF CELLULAR PHYSIOLOGY J2 - J CELL PHYSIOL VL - 236 PY - 2021 IS - 8 SP - 5592 EP - 5619 PG - 28 SN - 0021-9541 DO - 10.1002/jcp.30287 UR - https://m2.mtmt.hu/api/publication/31867767 ID - 31867767 N1 - Department of Biochemistry, School of Life Sciences (SLS), University of Hyderabad, Hyderabad, Telangana, India Department of Biology, University of Dayton, Dayton, OH, United States Export Date: 12 February 2021 CODEN: JCLLA Correspondence Address: Paddibhatla, I.; Department of Biochemistry, India; email: paddibhatla@gmail.com Department of Biochemistry, School of Life Sciences (SLS), University of Hyderabad, Hyderabad, Telangana, India Department of Biology, University of Dayton, Dayton, OH, United States Export Date: 14 February 2021 CODEN: JCLLA Correspondence Address: Paddibhatla, I.; Department of Biochemistry, India; email: paddibhatla@gmail.com AB - Hematopoiesis is a continuous phenomenon involving the formation of hematopoietic stem cells (HSCs) giving rise to diverse functional blood cells. This developmental process of hematopoiesis is evolutionarily conserved, yet comparably different in various model organisms. Vertebrate HSCs give rise to all types of mature cells of both the myeloid and the lymphoid lineages sequentially colonizing in different anatomical tissues. Signal transduction in HSCs facilitates their potency and specifies branching of lineages. Understanding the hematopoietic signaling pathways is crucial to gain insights into their deregulation in several blood-related disorders. The focus of the review is on hematopoiesis corresponding to different model organisms and pivotal role of indispensable hematopoietic pathways. We summarize and discuss the fundamentals of blood formation in both invertebrate and vertebrates, examining the requirement of key signaling nexus in hematopoiesis. Knowledge obtained from such comparative studies associated with developmental dynamics of hematopoiesis is beneficial to explore the therapeutic options for hematopoietic diseases. © 2021 Wiley Periodicals LLC LA - English DB - MTMT ER - TY - JOUR AU - Hirschhäuser, A. AU - van, Cann M. AU - Bogdan, S. TI - CK1α protects WAVE from degradation to regulate cell shape and motility in the immune response JF - JOURNAL OF CELL SCIENCE J2 - J CELL SCI VL - 134 PY - 2021 IS - 23 SN - 0021-9533 DO - 10.1242/jcs.258891 UR - https://m2.mtmt.hu/api/publication/32913576 ID - 32913576 N1 - Export Date: 30 June 2022 CODEN: JNCSA AB - The WAVE regulatory complex (WRC) is the main activator of the Arp2/3 complex, promoting lamellipodial protrusions in migrating cells. The WRC is basally inactive but can be activated by Rac1 and phospholipids, and through phosphorylation. However, the in vivo relevance of the phosphorylation of WAVE proteins remains largely unknown. Here, we identified casein kinase I alpha (CK1α) as a regulator of WAVE, thereby controlling cell shape and cell motility in Drosophila macrophages. CK1α binds and phosphorylates WAVE in vitro. Phosphorylation of WAVE by CK1α appears not to be required for activation but, rather, regulates its stability. Pharmacologic inhibition of CK1α promotes ubiquitin-dependent degradation of WAVE. Consistently, loss of Ck1α but not ck2 function phenocopies the depletion of WAVE. Phosphorylation-deficient mutations in the CK1α consensus sequences within the VCA domain of WAVE can neither rescue mutant lethality nor lamellipodium defects. By contrast, phosphomimetic mutations rescue all cellular and developmental defects. Finally, RNAi-mediated suppression of 26S proteasome or E3 ligase complexes substantially rescues lamellipodia defects in CK1α-depleted macrophages. Therefore, we conclude that basal phosphorylation of WAVE by CK1α protects it from premature ubiquitin-dependent degradation, thus promoting WAVE function in vivo. © 2021. Published by The Company of Biologists Ltd LA - English DB - MTMT ER - TY - JOUR AU - Huang, J. AU - Chen, J. AU - Fang, G. AU - Pang, L. AU - Zhou, S. AU - Zhou, Y. AU - Pan, Z. AU - Zhang, Q. AU - Sheng, Y. AU - Lu, Y. AU - Liu, Z. AU - Zhang, Y. AU - Li, G. AU - Shi, M. AU - Chen, X. AU - Zhan, S. TI - Two novel venom proteins underlie divergent parasitic strategies between a generalist and a specialist parasite JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 12 PY - 2021 IS - 1 SN - 2041-1723 DO - 10.1038/s41467-020-20332-8 UR - https://m2.mtmt.hu/api/publication/31871704 ID - 31871704 N1 - Institute of Insect Sciences, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Zhejiang University, Hangzhou, 310058, China CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China State Key Lab of Rice Biology, Zhejiang University, Hangzhou, 310058, China Export Date: 14 February 2021 Correspondence Address: Huang, J.; Institute of Insect Sciences, China; email: jhhuang@zju.edu.cn Correspondence Address: Chen, X.; Institute of Insect Sciences, China; email: xxchen@zju.edu.cn Correspondence Address: Zhan, S.; CAS Key Laboratory of Insect Developmental and Evolutionary Biology, China; email: szhan@sibs.ac.cn AB - Parasitoids are ubiquitous in natural ecosystems. Parasitic strategies are highly diverse among parasitoid species, yet their underlying genetic bases are poorly understood. Here, we focus on the divergent adaptation of a specialist and a generalist drosophilid parasitoids. We find that a novel protein (Lar) enables active immune suppression by lysing the host lymph glands, eventually leading to successful parasitism by the generalist. Meanwhile, another novel protein (Warm) contributes to a passive strategy by attaching the laid eggs to the gut and other organs of the host, leading to incomplete encapsulation and helping the specialist escape the host immune response. We find that these diverse parasitic strategies both originated from lateral gene transfer, followed with duplication and specialization, and that they might contribute to the shift in host ranges between parasitoids. Our results increase our understanding of how novel gene functions originate and how they contribute to host adaptation. © 2021, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Järvelä-Stölting, M. AU - Vesala, L. AU - Maasdorp, M.K. AU - Ciantar, J. AU - Rämet, M. AU - Valanne, S. TI - Proteasome α6 Subunit Negatively Regulates the JAK/STAT Pathway and Blood Cell Activation in Drosophila melanogaster JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 SN - 1664-3224 DO - 10.3389/fimmu.2021.729631 UR - https://m2.mtmt.hu/api/publication/32587143 ID - 32587143 N1 - Laboratory of Experimental Immunology, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland Research Unit for Pediatrics, Pediatric Neurology, Pediatric Surgery, Child Psychiatry, Dermatology, Clinical Genetics, Obstetrics and Gynecology, Otorhinolaryngology and Ophthalmology, Faculty of Medicine, University of Oulu, Oulu, Finland Medical Research Center Oulu, University of Oulu, Oulu, Finland Department of Children and Adolescents, Oulu University Hospital, University of Oulu, Oulu, Finland Export Date: 11 January 2022 Correspondence Address: Valanne, S.; Laboratory of Experimental Immunology, Finland; email: susanna.valanne@tuni.fi AB - JAK/STAT signaling regulates central biological functions such as development, cell differentiation and immune responses. In Drosophila, misregulated JAK/STAT signaling in blood cells (hemocytes) induces their aberrant activation. Using mass spectrometry to analyze proteins associated with a negative regulator of the JAK/STAT pathway, and by performing a genome-wide RNAi screen, we identified several components of the proteasome complex as negative regulators of JAK/STAT signaling in Drosophila. A selected proteasome component, Prosα6, was studied further. In S2 cells, Prosα6 silencing decreased the amount of the known negative regulator of the pathway, ET, leading to enhanced expression of a JAK/STAT pathway reporter gene. Silencing of Prosα6 in vivo resulted in activation of the JAK/STAT pathway, leading to the formation of lamellocytes, a specific hemocyte type indicative of hemocyte activation. This hemocyte phenotype could be partially rescued by simultaneous knockdown of either the Drosophila STAT transcription factor, or MAPKK in the JNK-pathway. Our results suggest a role for the proteasome complex components in the JAK/STAT pathway in Drosophila blood cells both in vitro and in vivo. Copyright © 2021 Järvelä-Stölting, Vesala, Maasdorp, Ciantar, Rämet and Valanne. LA - English DB - MTMT ER - TY - JOUR AU - Keehnen, N.L.P. AU - Fors, L. AU - Järver, P. AU - Spetz, A.-L. AU - Nylin, S. AU - Theopold, U. AU - Wheat, C.W. TI - A population genomic investigation of immune cell diversity and phagocytic capacity in a butterfly JF - GENES J2 - GENES-BASEL VL - 12 PY - 2021 IS - 2 PG - 17 SN - 2073-4425 DO - 10.3390/genes12020279 UR - https://m2.mtmt.hu/api/publication/32006424 ID - 32006424 N1 - Department of Zoology, Stockholm University, Svante Arrheniusväg 18b, Stockholm, S-106 91, Sweden Department of Molecular Biosciences, Wenner-Gren Institute, Stockholm University, Svante Arrheniusväg 20c, Stockholm, S-106 91, Sweden Export Date: 10 May 2021 Correspondence Address: Keehnen, N.L.P.; Department of Zoology, Svante Arrheniusväg 18b, Sweden AB - Insects rely on their innate immune system to successfully mediate complex interactions with their internal microbiota, as well as the microbes present in the environment. Given the variation in microbes across habitats, the challenges to respond to them are likely to result in local adaptations in the immune system. Here we focus upon phagocytosis, a mechanism by which pathogens and foreign particles are engulfed in order to be contained, killed, and processed. We investigated the phenotypic and genetic variation related to phagocytosis in two allopatric populations of the butterfly Pieris napi. Populations were found to differ in their hemocyte composition and overall phagocytic capability, driven by the increased phagocytic propensity of each cell type. Yet, genes annotated to phagocytosis showed no large genomic signal of divergence. However, a gene set enrichment analysis on significantly divergent genes identified loci involved in glutamine metabolism, which recently have been linked to immune cell differentiation in mammals. Together these results suggest that heritable variation in phagocytic capacity arises via a quantitative trait architecture with variation in genes affecting the activation and/or differentiation of phagocytic cells, suggesting them as potential candidate genes underlying these phenotypic differences. © 2021 by the authors. LA - English DB - MTMT ER - TY - JOUR AU - Mase, A. AU - Augsburger, J. AU - Brückner, K. TI - Macrophages and Their Organ Locations Shape Each Other in Development and Homeostasis – A Drosophila Perspective JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 9 PY - 2021 SN - 2296-634X DO - 10.3389/fcell.2021.630272 UR - https://m2.mtmt.hu/api/publication/32006156 ID - 32006156 N1 - Export Date: 10 May 2021 Correspondence Address: Brückner, K.; Department of Cell and Tissue Biology, United States; email: katja.brueckner@ucsf.edu Correspondence Address: Brückner, K.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, United States; email: katja.brueckner@ucsf.edu Correspondence Address: Brückner, K.; Cardiovascular Research Institute, United States; email: katja.brueckner@ucsf.edu AB - Across the animal kingdom, macrophages are known for their functions in innate immunity, but they also play key roles in development and homeostasis. Recent insights from single cell profiling and other approaches in the invertebrate model organism Drosophila melanogaster reveal substantial diversity among Drosophila macrophages (plasmatocytes). Together with vertebrate studies that show genuine expression signatures of macrophages based on their organ microenvironments, it is expected that Drosophila macrophage functional diversity is shaped by their anatomical locations and systemic conditions. In vivo evidence for diverse macrophage functions has already been well established by Drosophila genetics: Drosophila macrophages play key roles in various aspects of development and organogenesis, including embryogenesis and development of the nervous, digestive, and reproductive systems. Macrophages further maintain homeostasis in various organ systems and promote regeneration following organ damage and injury. The interdependence and interplay of tissues and their local macrophage populations in Drosophila have implications for understanding principles of organ development and homeostasis in a wide range of species. © Copyright © 2021 Mase, Augsburger and Brückner. LA - English DB - MTMT ER - TY - JOUR AU - Morin-Poulard, Ismael AU - Tian, Yushun AU - Vanzo, Nathalie AU - Crozatier, Michele TI - Drosophila as a Model to Study Cellular Communication Between the Hematopoietic Niche and Blood Progenitors Under Homeostatic Conditions and in Response to an Immune Stress JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 PG - 11 SN - 1664-3224 DO - 10.3389/fimmu.2021.719349 UR - https://m2.mtmt.hu/api/publication/32361805 ID - 32361805 N1 - Export Date: 19 January 2022 Correspondence Address: Crozatier, M.; MCD, France AB - In adult mammals, blood cells are formed from hematopoietic stem progenitor cells, which are controlled by a complex cellular microenvironment called "niche". Drosophila melanogaster is a powerful model organism to decipher the mechanisms controlling hematopoiesis, due both to its limited number of blood cell lineages and to the conservation of genes and signaling pathways throughout bilaterian evolution. Insect blood cells or hemocytes are similar to the mammalian myeloid lineage that ensures innate immunity functions. Like in vertebrates, two waves of hematopoiesis occur in Drosophila. The first wave takes place during embryogenesis. The second wave occurs at larval stages, where two distinct hematopoietic sites are identified: subcuticular hematopoietic pockets and a specialized hematopoietic organ called the lymph gland. In both sites, hematopoiesis is regulated by distinct niches. In hematopoietic pockets, sensory neurons of the peripheral nervous system provide a microenvironment that promotes embryonic hemocyte expansion and differentiation. In the lymph gland blood cells are produced from hematopoietic progenitors. A small cluster of cells called Posterior Signaling Centre (PSC) and the vascular system, along which the lymph gland develops, act collectively as a niche, under homeostatic conditions, to control the balance between maintenance and differentiation of lymph gland progenitors. In response to an immune stress such as wasp parasitism, lymph gland hematopoiesis is drastically modified and shifts towards emergency hematopoiesis, leading to increased progenitor proliferation and their differentiation into lamellocyte, a specific blood cell type which will neutralize the parasite. The PSC is essential to control this emergency response. In this review, we summarize Drosophila cellular and molecular mechanisms involved in the communication between the niche and hematopoietic progenitors, both under homeostatic and stress conditions. Finally, we discuss similarities between mechanisms by which niches regulate hematopoietic stem/progenitor cells in Drosophila and mammals. LA - English DB - MTMT ER - TY - JOUR AU - Mortimer, N.T. AU - Fischer, M.L. AU - Waring, A.L. AU - Pooja, K.R. AU - Kacsoh, B.Z. AU - Brantley, S.E. AU - Keebaugh, E.S. AU - Hill, J. AU - Lark, C. AU - Martin, J. AU - Bains, P. AU - Lee, J. AU - Vrailas-Mortimer, A.D. AU - Schlenke, T.A. TI - Extracellular matrix protein N-glycosylation mediates immune self-tolerance in Drosophila melanogaster JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 118 PY - 2021 IS - 39 SN - 0027-8424 DO - 10.1073/pnas.2017460118 UR - https://m2.mtmt.hu/api/publication/32876736 ID - 32876736 N1 - Export Date: 14 June 2022 CODEN: PNASA AB - In order to respond to infection, hosts must distinguish pathogens from their own tissues. This allows for the precise targeting of immune responses against pathogens and also ensures self-tolerance, the ability of the host to protect self tissues from immune damage. One way to maintain self-tolerance is to evolve a self signal and suppress any immune response directed at tissues that carry this signal. Here, we characterize the Drosophila tuSz1 mutant strain, which mounts an aberrant immune response against its own fat body. We demonstrate that this autoimmunity is the result of two mutations: 1) a mutation in the GCS1 gene that disrupts N-glycosylation of extracellular matrix proteins covering the fat body, and 2) a mutation in the Drosophila Janus Kinase ortholog that causes precocious activation of hemocytes. Our data indicate that N-glycans attached to extracellular matrix proteins serve as a self signal and that activated hemocytes attack tissues lacking this signal. The simplicity of this invertebrate self-recognition system and the ubiquity of its constituent parts suggests it may have functional homologs across animals. © 2021 National Academy of Sciences. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Ponrathnam, T. AU - Saini, R. AU - Banu, S. AU - Mishra, R.K. TI - Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 11 PY - 2021 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-021-81472-5 UR - https://m2.mtmt.hu/api/publication/31868380 ID - 31868380 N1 - Centre for Cellular and Molecular Biology, Hyderabad, Telangana 500007, India Academy of Scientific and Innovative Research (AcSIR), New Delhi, India Export Date: 12 February 2021 Correspondence Address: Mishra, R.K.; Centre for Cellular and Molecular BiologyIndia; email: mishra@ccmb.res.in Centre for Cellular and Molecular Biology, Hyderabad, Telangana 500007, India Academy of Scientific and Innovative Research (AcSIR), New Delhi, India Export Date: 14 February 2021 Correspondence Address: Mishra, R.K.; Centre for Cellular and Molecular BiologyIndia; email: mishra@ccmb.res.in AB - Hox genes are early determinants of cell identity along the anterior–posterior body axis across bilaterians. Several late non-homeotic functions of Hox genes have emerged in a variety of processes involved in organogenesis in several organisms, including mammals. Several studies have reported the misexpression of Hox genes in a variety of malignancies including acute myeloid leukemia. The Hox genes Dfd, Ubx, abd-A and Abd-B were overexpressed via the UAS-Gal4 system using Cg-Gal4, Lsp2-Gal4, He-Gal4 and HmlD3-Gal4 as specific drivers. Genetic interaction was tested by bringing overexpression lines in heterozygous mutant backgrounds of Polycomb and trithorax group factors. Larvae were visually scored for melanized bodies. Circulating hemocytes were quantified and tested for differentiation. Pupal lethality was assessed. Expression of Dfd, Ubx and abd-A, but not Abd-B in the hematopoietic compartment of Drosophila led to the appearance of circulating melanized bodies, an increase in cell number, cell-autonomous proliferation, and differentiation of hemocytes. Pupal lethality and melanized pseudo-tumors were suppressed in Psc1 and esc2 backgrounds while polycomb group member mutations Pc1 and Su(z)123 and trithorax group member mutation TrlR85 enhanced the phenotype. Dfd, Ubx and abd-A are leukemogenic. Mutations in Polycomb and trithorax group members modulate the leukemogenic phenotype. Our RNAseq of Cg-Gal4 > UAS-abd-A hemocytes may contain genes important to Hox gene induced leukemias. © 2021, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Ramroop, Johnny R. AU - Heavner, Mary Ellen AU - Razzak, Zubaidul H. AU - Govind, Shubha TI - A parasitoid wasp of Drosophila employs preemptive and reactive strategies to deplete its host's blood cells JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 17 PY - 2021 IS - 5 PG - 22 SN - 1553-7366 DO - 10.1371/journal.ppat.1009615 UR - https://m2.mtmt.hu/api/publication/32317577 ID - 32317577 N1 - Biology Department, The City College of the City University of New York, New York, NY, United States PhD Program in Biology, The Graduate Center, New York, NY, United States PhD Program in Biochemistry, The Graduate Center, New York, NY, United States Cited By :3 Export Date: 19 January 2022 Correspondence Address: Govind, S.; Biology Department, United States; email: sgovind@ccny.cuny.edu AB - The wasps Leptopilina heterotoma parasitize and ingest their Drosophila hosts. They produce extracellular vesicles (EVs) in the venom that are packed with proteins, some of which perform immune suppressive functions. EV interactions with blood cells of host larvae are linked to hematopoietic depletion, immune suppression, and parasite success. But how EVs disperse within the host, enter and kill hematopoietic cells are not well understood. Using an antibody marker for L. heterotoma EVs, we show that these parasite-derived structures are readily distributed within the hosts' hemolymphatic system. EVs converge around the tightly clustered cells of the posterior signaling center (PSC) of the larval lymph gland, a small hematopoietic organ in Drosophila. The PSC serves as a source of developmental signals in naive animals. In wasp-infected animals, the PSC directs the differentiation of lymph gland progenitors into lamellocytes. These lamellocytes are needed to encapsulate the wasp egg and block parasite development. We found that L. heterotoma infection disassembles the PSC and PSC cells disperse into the disintegrating lymph gland lobes. Genetically manipulated PSC-less lymph glands remain non-responsive and largely intact in the face of L. heterotoma infection. We also show that the larval lymph gland progenitors use the endocytic machinery to internalize EVs. Once inside, L. heterotoma EVs damage the Rab7- and LAMP-positive late endocytic and phagolysosomal compartments. Rab5 maintains hematopoietic and immune quiescence as Rab5 knockdown results in hematopoietic over-proliferation and ectopic lamellocyte differentiation. Thus, both aspects of anti-parasite immunity, i.e., (a) phagocytosis of the wasp's immune-suppressive EVs, and (b) progenitor differentiation for wasp egg encapsulation reside in the lymph gland. These results help explain why the lymph gland is specifically and precisely targeted for destruction. The parasite's simultaneous and multipronged approach to block cellular immunity not only eliminates blood cells, but also tactically blocks the genetic programming needed for supplementary hematopoietic differentiation necessary for host success. In addition to its known functions in hematopoiesis, our results highlight a previously unrecognized phagocytic role of the lymph gland in cellular immunity. EV-mediated virulence strategies described for L. heterotoma are likely to be shared by other parasitoid wasps; their understanding can improve the design and development of novel therapeutics and biopesticides as well as help protect biodiversity.Author summary Parasitoid wasps serve as biological control agents of agricultural insect pests and are worthy of study. Many parasitic wasps develop inside their hosts to emerge as free-living adults. To overcome the resistance of their hosts, parasitic wasps use varied and ingenious strategies such as mimicry, evasion, bioactive venom, virus-like particles, viruses, and extracellular vesicles (EVs). We describe the effects of a unique class of EVs containing virulence proteins and produced in the venom of wasps that parasitize fruit flies of Drosophila species. EVs from Leptopilina heterotoma are widely distributed throughout the Drosophila hosts' circulatory system after infection. They enter and kill macrophages by destroying the very same subcellular machinery that facilitates their uptake. An important protein in this process, Rab5, is needed to maintain the identity of the macrophage; when Rab5 function is reduced, macrophages turn into a different cell type called lamellocytes. Activities in the EVs can eliminate lamellocytes as well. EVs also interfere with the hosts' genetic program that promotes lamellocyte differentiation needed to block parasite development. Thus, wasps combine specific preemptive and reactive strategies to deplete their hosts of the very cells that would otherwise sequester and kill them. These findings have applied value in agricultural pest control and medical therapeutics. LA - English DB - MTMT ER - TY - JOUR AU - Szkalisity, Ábel AU - Piccinini, Filippo AU - Beleon, Attila AU - Balassa, Tamás AU - Varga, Gergely István AU - Migh, Ede AU - Molnár, Csaba AU - Paavolainen, Lassi AU - Timonen, Sanna AU - Banerjee, Indranil AU - Ikonen, Elina AU - Yamauchi, Yohei AU - Andó, István AU - Peltonen, Jaakko AU - Pietiäinen, Vilja AU - Honti, Viktor AU - Horváth, Péter TI - Regression plane concept for analysing continuous cellular processes with machine learning JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 12 PY - 2021 IS - 1 PG - 9 SN - 2041-1723 DO - 10.1038/s41467-021-22866-x UR - https://m2.mtmt.hu/api/publication/32023128 ID - 32023128 N1 - Cited By :2 Export Date: 14 June 2022 LA - English DB - MTMT ER - TY - JOUR AU - Trainor, J.E. AU - Pooja, K.R. AU - Mortimer, N.T. TI - Immune cell production is targeted by parasitoid wasp virulence in a drosophila–parasitoid wasp interaction JF - PATHOGENS J2 - PATHOGENS VL - 10 PY - 2021 IS - 1 SP - 1 EP - 16 PG - 16 SN - 2076-0817 DO - 10.3390/pathogens10010049 UR - https://m2.mtmt.hu/api/publication/31868552 ID - 31868552 N1 - Export Date: 12 February 2021 Correspondence Address: Mortimer, N.T.; School of Biological Sciences, United States; email: ntmorti@ilstu.edu Export Date: 14 February 2021 Correspondence Address: Mortimer, N.T.; School of Biological Sciences, United States; email: ntmorti@ilstu.edu AB - The interactions between Drosophila melanogaster and the parasitoid wasps that infect Drosophila species provide an important model for understanding host–parasite relationships. Following parasitoid infection, D. melanogaster larvae mount a response in which immune cells (hemocytes) form a capsule around the wasp egg, which then melanizes, leading to death of the parasitoid. Previous studies have found that host hemocyte load; the number of hemocytes available for the encapsulation response; and the production of lamellocytes, an infection induced hemocyte type, are major determinants of host resistance. Parasitoids have evolved various virulence mechanisms to overcome the immune response of the D. melanogaster host, including both active immune suppression by venom proteins and passive immune evasive mechanisms. We identified a previously undescribed parasitoid species, Asobara sp. AsDen, which utilizes an active virulence mechanism to infect D. melanogaster hosts. Asobara sp. AsDen infection inhibits host hemocyte expression of msn, a member of the JNK signaling pathway, which plays a role in lamellocyte production. Asobara sp. AsDen infection restricts the production of lamellocytes as assayed by hemocyte cell morphology and altered msn expression. Our findings suggest that Asobara sp. AsDen infection alters host signaling to suppress immunity. © 2021 by the authors. Li-censee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Wan, Bin AU - Belghazi, Maya AU - Lemauf, Severine AU - Poirie, Marylene AU - Gatti, Jean-Luc TI - Proteomics of purified lamellocytes from Drosophila melanogaster HopTum-l identifies new membrane proteins and networks involved in their functions JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 134 PY - 2021 PG - 21 SN - 0965-1748 DO - 10.1016/j.ibmb.2021.103584 UR - https://m2.mtmt.hu/api/publication/32358968 ID - 32358968 N1 - Université Côte d'Azur, INRAE, CNRS, Institute Sophia-Agrobiotech, Sophia Antipolis, France Institute of NeuroPhysiopathology (INP), UMR7051, CNRS, Aix-Marseille Université, Marseille, 13015, France Cited By :1 Export Date: 19 January 2022 CODEN: IBMBE Correspondence Address: Gatti, J.-L.; Sophia Agrobiotech Institute (ISA), Sophia Antipolis, France; email: jean-luc.gatti@inrae.fr AB - In healthy Drosophila melanogaster larvae, plasmatocytes and crystal cells account for 95% and 5% of the hemocytes, respectively. A third type of hemocytes, lamellocytes, are rare, but their number increases after oviposition by parasitoid wasps. The lamellocytes form successive layers around the parasitoid egg, leading to its encapsulation and melanization, and finally the death of this intruder. However, the total number of lamellocytes per larva remains quite low even after parasitoid infestation, making direct biochemical studies difficult. Here, we used the HopTum-l mutant strain that constitutively produces large numbers of lamellocytes to set up a purification method and analyzed their major proteins by 2D gel electrophoresis and their plasma membrane surface proteins by 1D SDS-PAGE after affinity purification. Mass spectrometry identified 430 proteins from 2D spots and 344 affinity-purified proteins from 1D bands, for a total of 639 unique proteins. Known lamellocyte markers such as PPO3 and the myospheroid integrin were among the components identified with specific chaperone proteins. Affinity purification detected other integrins, as well as a wide range of integrin-associated proteins involved in the formation and function of cell-cell junctions. Overall, the newly identified proteins indicate that these cells are highly adapted to the encapsulation process (recognition, motility, adhesion, signaling), but may also have several other physiological functions (such as secretion and internalization of vesicles) under different signaling pathways. These results provide the basis for further in vivo and in vitro studies of lamellocytes, including the development of new markers to identify coexisting populations and their respective origins and functions in Drosophila immunity. LA - English DB - MTMT ER - TY - JOUR AU - Yang, L. AU - Qiu, L.-M. AU - Fang, Q. AU - Stanley, D.W. AU - Ye, G.-Y. TI - Cellular and humoral immune interactions between Drosophila and its parasitoids JF - INSECT SCIENCE J2 - INSECT SCI VL - 28 PY - 2021 IS - 5 SP - 1208 EP - 1227 PG - 20 SN - 1672-9609 DO - 10.1111/1744-7917.12863 UR - https://m2.mtmt.hu/api/publication/31596046 ID - 31596046 N1 - State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China USDA Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, MO, United States Cited By :28 Export Date: 15 February 2024 Correspondence Address: Ye, G.-Y.; State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, China; email: chu@zju.edu.cn AB - The immune interactions occurring between parasitoids and their host insects, especially in Drosophila–wasp models, have long been the research focus of insect immunology and parasitology. Parasitoid infestation in Drosophila is counteracted by its multiple natural immune defense systems, which include cellular and humoral immunity. Occurring in the hemocoel, cellular immune responses involve the proliferation, differentiation, migration and spreading of host hemocytes and parasitoid encapsulation by them. Contrastingly, humoral immune responses rely more heavily on melanization and on the Toll, Imd and Jak/Stat immune pathways associated with antimicrobial peptides along with stress factors. On the wasps’ side, successful development is achieved by introducing various virulence factors to counteract immune responses of Drosophila. Some or all of these factors manipulate the host's immunity for successful parasitism. Here we review current knowledge of the cellular and humoral immune interactions between Drosophila and its parasitoids, focusing on the defense mechanisms used by Drosophila and the strategies evolved by parasitic wasps to outwit it. © 2020 Institute of Zoology, Chinese Academy of Sciences LA - English DB - MTMT ER - TY - JOUR AU - Zhou, Sicong AU - Lu, Yueqi AU - Wang, Ying AU - Chen, Jiani AU - Pang, Lan AU - Zhang, Qichao AU - Sheng, Yifeng AU - Liu, Zhiguo AU - Shi, Min AU - Chen, Xuexin AU - Huang, Jianhua TI - Comparative transcriptome analysis reveals a potential mechanism for host nutritional manipulation after parasitization by Leptopilina boulardi JF - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS J2 - COMP BIOCHEM PHYS D VL - 39 PY - 2021 PG - 11 SN - 1744-117X DO - 10.1016/j.cbd.2021.100862 UR - https://m2.mtmt.hu/api/publication/32424572 ID - 32424572 N1 - Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou, 310058, China Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Zhejiang University, Hangzhou, 310058, China State Key Lab of Rice Biology, Zhejiang University, Hangzhou, 310058, China Export Date: 19 January 2022 Correspondence Address: Huang, J.; Institute of Insect Sciences, China; email: jhhuang@zju.edu.cn AB - Parasitoids have been extensively found to manipulate nutrient amounts of their hosts to benefit their own development and survival, but the underlying mechanisms are largely unknown. Leptopilina boulardi (Hymenoptera: Figitidae) is a larval-pupal endoparasitoid wasp of Drosophila melanogaster whose survival relies on the nutrients provided by its Drosophila host. Here, we used RNA-seq to compare the gene expression levels of the host midgut at 24 h and 48 h post L. boulardi parasitization. We obtained 95 and 191 differentially expressed genes (DEGs) in the parasitized host midgut at 24 h and 48 h post L. boulardi parasitization, respectively. A KEGG analysis revealed that several metabolic pathways were significantly enriched in the upregulated DEGs, and these pathways included "starch and sucrose metabolism" and "galactose metabolism". A functional annotation analysis showed that four classes of genes involved in carbohydrate digestion process had increased expression levels in the midgut post L. boulardi parasitization than nonparasitized groups: glucosidase, mannosidase, chitinase and amylase. Genes involved in protein digestion process were also found among the DEGs, and most of these genes, which belonged to the metallopeptidase and serine-type endopeptidase families, were found at higher expression levels in the parasitized host midgut comparing with nonparasitized hosts. Moreover, some immune genes, particularly those involved in the Toll and Imd pathways, also exhibited high expression levels after L. boulardi parasitization. Our study provides large-scale transcriptome data and identifies sets of DEGs between parasitized and nonparasitized host midgut tissues at 24 h and 48 h post L. boulardi parasitization. These resources help improve our understanding of how parasitoid infection affects the nutrient components in the hosts. LA - English DB - MTMT ER - TY - JOUR AU - Bozler, Julianna AU - Kacsoh, Balint Z. AU - Bosco, Giovanni TI - Maternal Priming of Offspring Immune System in Drosophila JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 10 PY - 2020 IS - 1 SP - 165 EP - 175 PG - 11 SN - 2160-1836 DO - 10.1534/g3.119.400852 UR - https://m2.mtmt.hu/api/publication/31468836 ID - 31468836 N1 - Cited By :7 Export Date: 19 January 2022 Correspondence Address: Bozler, J.; Dartmouth Medical School, Vail 609, 74 College St, United States; email: Lita.Kacsoh@gmail.com AB - Immune priming occurs when a past infection experience leads to a more effective immune response upon a secondary exposure to the infection or pathogen. In some instances, parents are able to transmit immune priming to their offspring, creating a subsequent generation with a superior immune capability, through processes that are not yet fully understood. Using a parasitoid wasp, which infects larval stages of Drosophila melanogaster, we describe an example of an intergenerational inheritance of immune priming. This phenomenon is anticipatory in nature and does not rely on parental infection, but rather, when adult fruit flies are cohabitated with a parasitic wasp, they produce offspring that are more capable of mounting a successful immune response against a parasitic macro-infection. This increase in offspring survival correlates with a more rapid induction of lamellocytes, a specialized immune cell. RNA-sequencing of the female germline identifies several differentially expressed genes following wasp exposure, including the peptiodoglycan recognition protein-LB (PGRP-LB). We find that genetic manipulation of maternal PGRP-LB identifies this gene as a key element in this intergenerational phenotype. LA - English DB - MTMT ER - TY - JOUR AU - Cattenoz, Pierre B. AU - Sakr, Rosy AU - Pavlidaki, Alexia AU - Delaporte, Claude AU - Riba, Andrea AU - Molina, Nacho AU - Hariharan, Nivedita AU - Mukherjee, Tina AU - Giangrande, Angela TI - Temporal specificity and heterogeneity ofDrosophilaimmune cells JF - EMBO JOURNAL J2 - EMBO J VL - 39 PY - 2020 IS - 12 PG - 25 SN - 0261-4189 DO - 10.15252/embj.2020104486 UR - https://m2.mtmt.hu/api/publication/31460653 ID - 31460653 N1 - Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France Centre National de la Recherche Scientifique, UMR7104, Illkirch, France Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France Université de Strasbourg, Illkirch, France Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, India The University of Trans-disciplinary Health Sciences and Technology, Bangalore, India Cited By :31 Export Date: 19 January 2022 CODEN: EMJOD Correspondence Address: Cattenoz, P.B.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Centre National de la Recherche Scientifique, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Centre National de la Recherche Scientifique, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Institut National de la Santé et de la Recherche Médicale, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut National de la Santé et de la Recherche Médicale, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Université de StrasbourgFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Université de StrasbourgFrance; email: angela@igbmc.fr AB - Immune cells provide defense against non-self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity ofDrosophilaimmune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single-cell RNA sequencing, we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct processes, e.g., proliferation, phagocytosis, metabolic homeostasis, and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation, which triggers the differentiation of a novel hemocyte type, the lamellocyte. This first molecular atlas of hemocytes provides insights and paves the way to study the biology of theDrosophilaimmune cells in physiological and pathological conditions. LA - English DB - MTMT ER - TY - JOUR AU - Cho, B. AU - Yoon, S.-H. AU - Lee, D. AU - Koranteng, F. AU - Tattikota, S.G. AU - Cha, N. AU - Shin, M. AU - Do, H. AU - Hu, Y. AU - Oh, S.Y. AU - Lee, D. AU - Vipin, Menon A. AU - Moon, S.J. AU - Perrimon, N. AU - Nam, J.-W. AU - Shim, J. TI - Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 11 PY - 2020 IS - 1 SN - 2041-1723 DO - 10.1038/s41467-020-18135-y UR - https://m2.mtmt.hu/api/publication/31868487 ID - 31868487 N1 - Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, South Korea Department of Genetics, Harvard Medical School, Boston, MA 02115, United States Department of Oral Biology, Yonsei University, College of Dentistry, Seoul, 03722, South Korea Howard Hughes Medical Institute, Boston, MA 02115, United States Research Institute for Natural Sciences, Hanyang University, Seoul, 04736, South Korea Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul, 04736, South Korea Cited By :4 Export Date: 12 February 2021 Correspondence Address: Nam, J.-W.; Department of Life Sciences, South Korea; email: jwnam@hanyang.ac.kr Correspondence Address: Shim, J.; Department of Life Sciences, South Korea; email: jshim@hanyang.ac.kr Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, South Korea Department of Genetics, Harvard Medical School, Boston, MA 02115, United States Department of Oral Biology, Yonsei University, College of Dentistry, Seoul, 03722, South Korea Howard Hughes Medical Institute, Boston, MA 02115, United States Research Institute for Natural Sciences, Hanyang University, Seoul, 04736, South Korea Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul, 04736, South Korea Cited By :4 Export Date: 14 February 2021 Correspondence Address: Nam, J.-W.; Department of Life Sciences, South Korea; email: jwnam@hanyang.ac.kr Correspondence Address: Shim, J.; Department of Life Sciences, South Korea; email: jshim@hanyang.ac.kr Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, South Korea Department of Genetics, Harvard Medical School, Boston, MA 02115, United States Department of Oral Biology, Yonsei University, College of Dentistry, Seoul, 03722, South Korea Howard Hughes Medical Institute, Boston, MA 02115, United States Research Institute for Natural Sciences, Hanyang University, Seoul, 04736, South Korea Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul, 04736, South Korea Cited By :10 Export Date: 10 May 2021 Correspondence Address: Nam, J.-W.; Department of Life Sciences, South Korea; email: jwnam@hanyang.ac.kr Correspondence Address: Shim, J.; Department of Life Sciences, South Korea; email: jshim@hanyang.ac.kr AB - The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution. © 2020, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Lerner, Zita AU - Magyar, Lilla Brigitta AU - Soós, Bálint AU - Tóth, Renáta AU - Kristó, Ildikó AU - Vilmos, Péter AU - Juhász, Gábor AU - Kovács, Attila Lajos AU - Hegedűs, Zoltán AU - Sensen, Christoph W. AU - Kurucz, Judit Éva AU - Andó, István TI - Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid Zaprionus indianus JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 12 PY - 2020 IS - 3 SP - 257 EP - 272 PG - 16 SN - 1662-811X DO - 10.1159/000502646 UR - https://m2.mtmt.hu/api/publication/30819399 ID - 30819399 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Dziedziech, Alexis AU - Shivankar, Sai AU - Theopold, Ulrich TI - Drosophilamelanogaster Responses against Entomopathogenic Nematodes: Focus on Hemolymph Clots JF - INSECTS J2 - INSECTS VL - 11 PY - 2020 IS - 1 PG - 11 SN - 2075-4450 DO - 10.3390/insects11010062 UR - https://m2.mtmt.hu/api/publication/31470099 ID - 31470099 N1 - Cited By :5 Export Date: 19 January 2022 Correspondence Address: Theopold, U.; Department of Molecular Biosciences, Sweden; email: uli.theopold@su.se AB - Several insect innate immune mechanisms are activated in response to infection by entomopathogenic nematodes (EPNs). In this review, we focus on the coagulation of hemolymph, which acts to stop bleeding after injury and prevent access of pathogens to the body cavity. After providing a general overview of invertebrate coagulation systems, we discuss recent findings in Drosophila melanogaster which demonstrate that clots protect against EPN infections. Detailed analysis at the cellular level provided insight into the kinetics of the secretion of Drosophila coagulation factors, including non-classical modes of secretion. Roughly, clot formation can be divided into a primary phase in which crosslinking of clot components depends on the activity of Drosophila transglutaminase and a secondary, phenoloxidase (PO)-dependent phase, characterized by further hardening and melanization of the clot matrix. These two phases appear to play distinct roles in two commonly used EPN infection models, namely Heterorhabditis bacteriophora and Steinernema carpocapsae. Finally, we discuss the implications of the coevolution between parasites such as EPNs and their hosts for the dynamics of coagulation factor evolution. LA - English DB - MTMT ER - TY - JOUR AU - Fu, Yulong AU - Huang, Xiaohu AU - Zhang, Peng AU - van de Leemput, Joyce AU - Han, Zhe TI - Single-cell RNA sequencing identifies novel cell types in Drosophila blood JF - JOURNAL OF GENETICS AND GENOMICS J2 - J GENET GENOMICS VL - 47 PY - 2020 IS - 4 SP - 175 EP - 186 PG - 12 SN - 1673-8527 DO - 10.1016/j.jgg.2020.02.004 UR - https://m2.mtmt.hu/api/publication/31469242 ID - 31469242 N1 - Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, United States Divisions of Immunotherapy, University of Maryland School of Medicine, Baltimore, MD 21201, United States Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, United States Cited By :21 Export Date: 19 January 2022 Correspondence Address: Han, Z.; Center for Precision Disease Modeling, 670 West Baltimore Street, 4052 HSF3, United States; email: zhan@som.umaryland.edu AB - Drosophila has been extensively used to model the human blood-immune system, as both systems share many developmental and immune response mechanisms. However, while many human blood cell types have been identified, only three were found in flies: plasmatocytes, crystal cells and lamellocytes. To better understand the complexity of fly blood system, we used single-cell RNA sequencing technology to generate comprehensive gene expression profiles for Drosophila circulating blood cells. In addition to the known cell types, we identified two new Drosophila blood cell types: thanacytes and primocytes. Thanacytes, which express many stimulus response genes, are involved in distinct responses to different types of bacteria. Primocytes, which express cell fate commitment and signaling genes, appear to be involved in keeping stem cells in the circulating blood. Furthermore, our data revealed four novel plasmatocyte subtypes (Ppn(+), CAH7(+), Lsp(+) and reservoir plasmatocytes), each with unique molecular identities and distinct predicted functions. We also identified cross-species markers from Drosophila hemocytes to human blood cells. Our analysis unveiled a more complex Drosophila blood system and broadened the scope of using Drosophila to model human blood system in development and disease. Copyright (C) 2020, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Horton, Miles B. AU - Hawkins, Edwin D. AU - Heinzel, Susanne AU - Hodgkin, Philip D. TI - Speculations on the evolution of humoral adaptive immunity JF - IMMUNOLOGY AND CELL BIOLOGY J2 - IMMUNOL CELL BIOL VL - 98 PY - 2020 IS - 6 SP - 439 EP - 448 PG - 10 SN - 0818-9641 DO - 10.1111/imcb.12323 UR - https://m2.mtmt.hu/api/publication/31468406 ID - 31468406 N1 - Division of Immunology, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia Cited By :2 Export Date: 19 January 2022 CODEN: ICBIE Correspondence Address: Hodgkin, P.D.; Division of Immunology, Australia; email: hodgkin@wehi.edu.au Correspondence Address: Hodgkin, P.D.; Department of Medical Biology, Australia; email: hodgkin@wehi.edu.au AB - The protection of a multicellular organism from infection, at both cell and humoral levels, has been a tremendous driver of gene selection and cellular response strategies. Here we focus on a critical event in the development of humoral immunity: The transition from principally innate responses to a system of adaptive cell selection, with all the attendant mechanical problems that must be solved in order for it to work effectively. Here we review recent advances, but our major goal is to highlight that the development of adaptive immunity resulted from the adoption, reuse and repurposing of an ancient, autonomous cellular program that combines and exploits three titratable cellular fate timers. We illustrate how this common cell machinery recurs and appears throughout biology, and has been essential for the evolution of complex organisms, at many levels of scale. LA - English DB - MTMT ER - TY - JOUR AU - Hu, Jian AU - Du, Yan AU - Meng, Meng AU - Dong, Yipei AU - Peng, Jiewen TI - Development of two continuous hemocyte cell sublines in the Asian corn borerOstrinia furnacalisand the identification of molecular markers for hemocytes JF - INSECT SCIENCE J2 - INSECT SCI PY - 2020 PG - 17 SN - 1672-9609 DO - 10.1111/1744-7917.12854 UR - https://m2.mtmt.hu/api/publication/31686512 ID - 31686512 N1 - School of Agriculture, Sun Yat-Sen University, Guangzhou, 510275, China State key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China Export Date: 19 January 2022 Correspondence Address: Hu, J.; School of Agriculture, China; email: lsshj@mail.sysu.edu.cn AB - Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects, but it is difficult to distinguish between them in immune reactions. Based on the hemocyte cell line SYSU-OfHe-C established at our lab, two cell sublines, SYSU-OfHe-C Granulocyte (Gr cells) and SYSU-OfHe-C Plasmatocyte (Pl cells), which possess the morphological characteristics of granulocytes and plasmatocytes, respectively, were established. Gr and Pl cells showed different behaviors in immune reactions, such as spreading, phagocytosis and encapsulation. Pl cells were easier to spread, but Gr cells tended to undergo aggregation, indicating that they may take different strategies to clear foreign objects. These results also suggested that granulocytes and plasmatocytes may express some different proteins. By comparing the gene expression in cells from the two sublines, 1662 differentially expressed genes were identified, and 13 out of 30 transmembrane proteins highly expressed in Pl cells (six) or Gr cells (seven) were further screened and confirmed by reverse-transcription polymerase chain reaction (PCR). Finally, three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR analysis. These genes may potentially be used as molecular markers to distinguish between granulocytes and plasmatocytes inOstrinia furnacalis, and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on. LA - English DB - MTMT ER - TY - JOUR AU - Lan, Wenwen AU - Liu, Sumin AU - Zhao, Long AU - Su, Ying TI - Regulation ofDrosophilaHematopoiesis in Lymph Gland: From a Developmental Signaling Point of View JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 15 PG - 15 SN - 1661-6596 DO - 10.3390/ijms21155246 UR - https://m2.mtmt.hu/api/publication/31686813 ID - 31686813 N1 - Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003, China College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China Fisheries College, Ocean University of China, Qingdao, 266003, China Cited By :1 Export Date: 19 January 2022 Correspondence Address: Zhao, L.; Institute of Evolution & Marine Biodiversity, China; email: zhaolong@ouc.edu.cn Correspondence Address: Zhao, L.; Fisheries College, China; email: zhaolong@ouc.edu.cn Correspondence Address: Su, Y.; Institute of Evolution & Marine Biodiversity, China; email: suying@ouc.edu.cn Correspondence Address: Su, Y.; College of Marine Life Sciences, China; email: suying@ouc.edu.cn AB - TheDrosophilahematopoietic system is becoming increasingly attractive for its simple blood cell lineage and its developmental and functional parallels with the vertebrate system. As the dedicated organ forDrosophilalarval hematopoiesis, the lymph gland harbors both multipotent stem-like progenitor cells and differentiated blood cells. The balance between progenitor maintenance and differentiation in the lymph gland must be precisely and tightly controlled. Multiple developmental signaling pathways, such as Notch, Hedgehog, and Wnt/Wingless, have been demonstrated to regulate the hematopoietic processes in the lymph gland. Focusing on blood cell maintenance and differentiation, this article summarizes the functions of several classic developmental signaling pathways for lymph gland growth and patterning, highlighting the important roles of developmental signaling during lymph gland development as well asDrosophilalarval hematopoiesis. LA - English DB - MTMT ER - TY - JOUR AU - Madhwal, S. AU - Shin, M. AU - Kapoor, A. AU - Goyal, M. AU - Joshi, M.K. AU - Rehman, P.M.U. AU - Gor, K. AU - Shim, J. AU - Mukherjee, T. TI - Metabolic control of cellular immune-competency by odors in drosophila JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 SP - 1 EP - 93 PG - 93 SN - 2050-084X DO - 10.7554/ELIFE.60376 UR - https://m2.mtmt.hu/api/publication/31868549 ID - 31868549 N1 - Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, Bangalore, 560065, India Department of Life Science, College of Natural Science, Hanyang University, Seoul, 04763, South Korea Vellore Institute of Technology, Katpadi Road, Vellore, Tamil Nadu 632014, India Research Institute for Natural Science, Hanyang University, Seoul, 04763, South Korea Manipal Academy of Higher Education, Manipal, Karnataka 576104, India The University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka 560064, India Export Date: 12 February 2021 Correspondence Address: Shim, J.; Department of Life Science, South Korea; email: jshim@hanyang.ac.kr Correspondence Address: Mukherjee, T.; Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, India; email: tinam@instem.res.in Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, Bangalore, 560065, India Department of Life Science, College of Natural Science, Hanyang University, Seoul, 04763, South Korea Vellore Institute of Technology, Katpadi Road, Vellore, Tamil Nadu 632014, India Research Institute for Natural Science, Hanyang University, Seoul, 04763, South Korea Manipal Academy of Higher Education, Manipal, Karnataka 576104, India The University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka 560064, India Export Date: 14 February 2021 Correspondence Address: Shim, J.; Department of Life Science, South Korea; email: jshim@hanyang.ac.kr Correspondence Address: Mukherjee, T.; Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, India; email: tinam@instem.res.in LA - English DB - MTMT ER - TY - JOUR AU - Panettieri, Silvio AU - Paddibhatla, Indira AU - Chou, Jennifer AU - Rajwani, Roma AU - Moore, Rebecca S. AU - Goncharuk, Tamara AU - John, George AU - Govind, Shubha TI - Discovery of aspirin-triggered eicosanoid-like mediators in a Drosophila metainflammation blood tumor model JF - JOURNAL OF CELL SCIENCE J2 - J CELL SCI VL - 133 PY - 2020 IS - 5 PG - 11 SN - 0021-9533 DO - 10.1242/jcs.236141 UR - https://m2.mtmt.hu/api/publication/31509173 ID - 31509173 N1 - Department of Chemistry & Biochemistry, City College of New York, New York, NY 10031, United States Graduate Center, City University of New York, 365 Fifth Avenue, New York, NY 10016, United States Biology Department, City College of New York, 160 Convent Avenue, New York, NY 10031, United States Department of Biochemistry, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, 500046, India Cited By :7 Export Date: 19 January 2022 CODEN: JNCSA Correspondence Address: John, G.; Department of Chemistry & Biochemistry, United States; email: gjohn@ccny.cuny.edu AB - Epidemiologic studies have linked the use of aspirin to a decline in chronic inflammation that underlies many human diseases, including some cancers. Aspirin reduces the levels of cyclooxygenase-mediated pro-inflammatory prostaglandins, promotes the production of proresolution molecules, and triggers the production of anti-inflammatory electrophilic mono-oxygenated (EFOX) lipid mediators. We investigated the effects of aspirin in fruit fly models of chronic inflammation. Ectopic Toll/NF-kappa B and JAK/STAT signaling in mutant D. melanogaster results in overproliferation of hematopoietic blood progenitors resulting in the formation of granuloma-like tumors. Ectopic JAK-STAT signaling also leads to metabolic inflammation. We report that aspirin-treated mutant flies experience reduction in metabolic inflammation, mitosis, ectopic immune signaling, and macrophage infiltration. Moreover, these flies synthesize 13-HODE, and aspirin triggers 13-oxoODE (13-EFOX-L-2) production. Providing the precursor of 13-HODE, linoleic acid, or performing targeted knockdown of the transcription factor STAT in inflammatory blood cells, boosts 13-EFOX-L-2 levels while decreasing metabolic inflammation. Thus, hematopoietic cells regulate metabolic inflammation in flies, and their effects can be reversed by pharmaceutical or dietary intervention, suggesting deep phylogenetic conservation in the ability of animals to resolve inflammation and repair tissue damage. These findings can help identify novel treatment targets in humans. LA - English DB - MTMT ER - TY - JOUR AU - Potts, Rashaun AU - King, Jonas G. AU - Pietri, Jose E. TI - Ex vivo characterization of the circulating hemocytes of bed bugs and their responses to bacterial exposure JF - JOURNAL OF INVERTEBRATE PATHOLOGY J2 - J INVERTEBR PATHOL VL - 174 PY - 2020 PG - 10 SN - 0022-2011 DO - 10.1016/j.jip.2020.107422 UR - https://m2.mtmt.hu/api/publication/31509180 ID - 31509180 N1 - University of South Dakota, Sanford School of Medicine, Division of Basic Biomedical Sciences, Vermillion, SD, United States Mississippi State University, Department of Biochemistry, Molecular Biology, Entomology & Plant Pathology, Starkville, MS, United States Cited By :2 Export Date: 19 January 2022 CODEN: JIVPA Correspondence Address: Pietri, J.E.; University of South Dakota, United States; email: Jose.Pietri@usd.edu AB - Bed bugs (Cimex spp.) are urban pests of global importance. Knowledge of the immune system of bed bugs has implications for understanding their susceptibility to biological control agents, their potential to transmit human pathogens, and the basic comparative immunology of insects. Nonetheless, the immunological repertoire of the family Cimicidae remains poorly characterized. Here, we use microscopy, flow cytometry, and RNA sequencing to provide a basal characterization of the circulating hemocytes of the common bed bug, Cimex lectularius. We also examine the responses of these specialized cells to E. coli exposure using the same techniques. Our results show that circulating hemocytes are comprised of at least four morphologically distinct cell types that are capable of phagocytosis, undergo degranulation, and exhibit additional markers of activation following stimulation, including size shift and DNA replication. Furthermore, transcriptomic profiling reveals expression of predicted Toll/IMD signaling pathway components, antimicrobial effectors and other potentially immunoresponsive genes in these cells. Together, our data demonstrate the conservation of several canonical cellular immune responses in the common bed bug and provide a foundation for additional mechanistic immunological studies with specific pathogens of interest. LA - English DB - MTMT ER - TY - JOUR AU - Ramond, Elodie AU - Dudzic, Jan Paul AU - Lemaitre, Bruno TI - Comparative RNA-Seq analyses ofDrosophilaplasmatocytes reveal gene specific signatures in response to clean injury and septic injury JF - PLOS ONE J2 - PLOS ONE VL - 15 PY - 2020 IS - 6 PG - 27 SN - 1932-6203 DO - 10.1371/journal.pone.0235294 UR - https://m2.mtmt.hu/api/publication/31468577 ID - 31468577 N1 - Cited By :9 Export Date: 19 January 2022 CODEN: POLNC Correspondence Address: Ramond, E.; Global Health Institute, Switzerland; email: elodie.ramond@inserm.fr AB - Drosophila melanogaster's blood cells (hemocytes) play essential roles in wound healing and are involved in clearing microbial infections. Here, we report the transcriptional changes of larval plasmatocytes after clean injury or infection with the Gram-negative bacteriumEscherichia colior the Gram-positive bacteriumStaphylococcus aureuscompared to hemocytes recovered from unchallenged larvae via RNA-Sequencing. This study reveals 676 differentially expressed genes (DEGs) in hemocytes from clean injury samples compared to unchallenged samples, and 235 and 184 DEGs inE.coliandS.aureussamples respectively compared to clean injury samples. The clean injury samples showed enriched DEGs for immunity, clotting, cytoskeleton, cell migration, hemocyte differentiation, and indicated a metabolic reprogramming to aerobic glycolysis, a well-defined metabolic adaptation observed in mammalian macrophages. Microbial infections trigger significant transcription of immune genes, with significant differences between theE.coliandS.aureussamples suggesting that hemocytes have the ability to engage various programs upon infection. Collectively, our data bring new insights onDrosophilahemocyte function and open the route to post-genomic functional analysis of the cellular immune response. LA - English DB - MTMT ER - TY - JOUR AU - Ramond, Elodie AU - Petrignani, Bianca AU - Dudzic, Jan Paul AU - Boquete, Jean-Philippe AU - Poidevin, Mickael AU - Kondo, Shu AU - Lemaitre, Bruno TI - The adipokine NimrodB5 regulates peripheral hematopoiesis in Drosophila JF - FEBS JOURNAL J2 - FEBS J PY - 2020 PG - 28 SN - 1742-464X DO - 10.1111/febs.15237 UR - https://m2.mtmt.hu/api/publication/31469236 ID - 31469236 N1 - Global Health Institute, School of Life Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland Centre de Génétique Moléculaire CNRS, Université Pierre et Marie Curie, Gif-sur-Yvette, France Invertebrate Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima, Japan Cited By :8 Export Date: 19 January 2022 CODEN: FJEOA Correspondence Address: Lemaitre, B.; Global Health Institute, Switzerland; email: bruno.lemaitre@epfl.ch AB - In animals, growth is regulated by the complex interplay between paracrine and endocrine signals. When food is scarce, tissues compete for nutrients, leading to critical resource allocation and prioritization. Little is known about how the immune system maturation is coordinated with the growth of other tissues. Here, we describe a signaling mechanism that regulates the number of hemocytes (blood cells) according to the nutritional state of the Drosophila larva. Specifically, we found that a secreted protein, NimB5, is produced in the fat body upon nutrient scarcity downstream of metabolic sensors and ecdysone signaling. NimB5 is then secreted and binds to hemocytes to down-regulate their proliferation and adhesion. Blocking this signaling loop results in conditional lethality when larvae are raised on a poor diet, due to excessive hemocyte numbers and insufficient energy storage. Similar regulatory mechanisms shaping the immune system in response to nutrient availability are likely to be widespread in animals. LA - English DB - MTMT ER - TY - JOUR AU - Salminen, T.S. AU - Vale, P.F. TI - Drosophila as a Model System to Investigate the Effects of Mitochondrial Variation on Innate Immunity JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 11 PY - 2020 SN - 1664-3224 DO - 10.3389/fimmu.2020.00521 UR - https://m2.mtmt.hu/api/publication/31871705 ID - 31871705 N1 - Cited By :2 Export Date: 14 February 2021 Correspondence Address: Salminen, T.S.; School of Biological Sciences, United Kingdom; email: tiina.susanna.salminen@gmail.com AB - Understanding why the response to infection varies between individuals remains one of the major challenges in immunology and infection biology. A substantial proportion of this heterogeneity can be explained by individual genetic differences which result in variable immune responses, and there are many examples of polymorphisms in nuclear-encoded genes that alter immunocompetence. However, how immunity is affected by genetic polymorphism in an additional genome, inherited maternally inside mitochondria (mtDNA), has been relatively understudied. Mitochondria are increasingly recognized as important mediators of innate immune responses, not only because they are the main source of energy required for costly immune responses, but also because by-products of mitochondrial metabolism, such as reactive oxygen species (ROS), may have direct microbicidal action. Yet, it is currently unclear how naturally occurring variation in mtDNA contributes to heterogeneity in infection outcomes. In this review article, we describe potential sources of variation in mitochondrial function that may arise due to mutations in vital nuclear and mitochondrial components of energy production or due to a disruption in mito-nuclear crosstalk. We then highlight how these changes in mitochondrial function can impact immune responses, focusing on their effects on ATP- and ROS-generating pathways, as well as immune signaling. Finally, we outline how being a powerful and genetically tractable model of infection, immunity and mitochondrial genetics makes the fruit fly Drosophila melanogaster ideally suited to dissect mitochondrial effects on innate immune responses to infection. © Copyright © 2020 Salminen and Vale. LA - English DB - MTMT ER - TY - JOUR AU - Shin, Mingyu AU - Cha, Nuri AU - Koranteng, Ferdinand AU - Cho, Bumsik AU - Shim, Jiwon TI - Subpopulation of Macrophage-Like Plasmatocytes Attenuates Systemic Growth via JAK/STAT in the Drosophila Fat Body JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 11 PY - 2020 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2020.00063 UR - https://m2.mtmt.hu/api/publication/31467726 ID - 31467726 N1 - Department of Life Science, College of Natural Science, Hanyang University, Seoul, South Korea Research Institute for Natural Science, College of Natural Science, Hanyang University, Seoul, South Korea Research Institute for Convergence of Basic Sciences, College of Natural Science, Hanyang University, Seoul, South Korea Cited By :6 Export Date: 19 January 2022 Correspondence Address: Shim, J.; Department of Life Science, South Korea; email: jshim@hanyang.ac.kr AB - Drosophila hemocytes, like those of mammals, are given rise from two distinctive phases during both the embryonic and larval hematopoiesis. Embryonically derived hemocytes, mostly composed of macrophage-like plasmatocytes, are largely identified by genetic markers. However, the cellular diversity and distinct functions of possible subpopulations within plasmatocytes have not been explored in Drosophila larvae. Here, we show that larval plasmatocytes exhibit differential expressions of Hemolectin (Hml) and Peroxidasin (Pxn) during development. Moreover, removal of plasmatocytes by overexpressing pro-apoptotic genes, hid and reaper in Hml-positive plasmatocytes, feeding high sucrose diet, or wasp infestation results in increased circulating hemocytes that are Hml-negative. Interestingly these Hml-negative plasmatocytes retain Pxn expression, and animals expressing Hml-negative and Pxn-positive subtype largely attenuate growth and abrogate metabolism. Furthermore, elevated levels of a cytokine, unpaired 3, are detected when Hml-positive hemocytes are ablated, which in turn activates JAK/STAT activity in several tissues including the fat body. Finally, we observed that insulin signaling is inhibited in this background, which can be recovered by concurrent loss of upd3. Overall, this study highlights heterogeneity in Drosophila plasmatocytes and a functional plasticity of each subtype, which reaffirms extension of their role beyond immunity into metabolic regulation for cooperatively maintaining internal homeostatic balance. LA - English DB - MTMT ER - TY - JOUR AU - Tattikota, Sudhir Gopal AU - Cho, Bumsik AU - Liu, Yifang AU - Hu, Yanhui AU - Barrera, Victor AU - Steinbaugh, Michael J. AU - Yoon, Sang-Ho AU - Comjean, Aram AU - Li, Fangge AU - Dervis, Franz AU - Hung, Ruei-Jiun AU - Nam, Jin-Wu AU - Sui, Shannan Ho AU - Shim, Jiwon AU - Perrimon, Norbert TI - A single-cell survey of Drosophila blood JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 PG - 35 SN - 2050-084X DO - 10.7554/eLife.54818 UR - https://m2.mtmt.hu/api/publication/31468416 ID - 31468416 N1 - Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States Department of Life Science, Hanyang University, Seoul, South Korea Harvard TH Chan Bioinformatics Core, Boston, United States Howard Hughes Medical Institute, Boston, United States Cited By :37 Export Date: 19 January 2022 Correspondence Address: Tattikota, S.G.; Department of Genetics, United States; email: sudhir_gt@hms.harvard.edu Correspondence Address: Perrimon, N.; Department of Genetics, United States; email: perrimon@receptor.med.harvard.edu Correspondence Address: Perrimon, N.; Howard Hughes Medical InstituteUnited States; email: perrimon@receptor.med.harvard.edu AB - Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions. LA - English DB - MTMT ER - TY - JOUR AU - Vesala, L. AU - Hultmark, D. AU - Valanne, S. TI - Editorial: Recent Advances in Drosophila Cellular and Humoral Innate Immunity JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 11 PY - 2020 SN - 1664-3224 DO - 10.3389/fimmu.2020.598618 UR - https://m2.mtmt.hu/api/publication/32601705 ID - 32601705 N1 - Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland Department of Molecular Biology, Umeå University, Umeå, Sweden Export Date: 19 January 2022 Correspondence Address: Valanne, S.; Faculty of Medicine and Health Technology, Finland; email: susanna.valanne@tuni.fi LA - English DB - MTMT ER - TY - JOUR AU - Vesala, Laura AU - Hultmark, Dan AU - Valanne, Susanna TI - Recent Advances in Drosophila Cellular and Humoral Innate Immunity JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 11 PY - 2020 PG - 3 SN - 1664-3224 DO - 10.3389/fimmu.2020.598618 UR - https://m2.mtmt.hu/api/publication/31690514 ID - 31690514 LA - English DB - MTMT ER - TY - JOUR AU - Wang, Ze-Hua AU - Zhou, Yue-Nan AU - Ye, Xi-Qian AU - Wu, Xiao-Tong AU - Yang, Pei AU - Shi, Min AU - Huang, Jian-Hua AU - Chen, Xue-Xin TI - CLP gene family, a new gene family of Cotesia vestalis bracovirus inhibits melanization of Plutella xylostella hemolymph JF - INSECT SCIENCE J2 - INSECT SCI PY - 2020 PG - 15 SN - 1672-9609 DO - 10.1111/1744-7917.12883 UR - https://m2.mtmt.hu/api/publication/31686183 ID - 31686183 N1 - Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China Zhejiang Provincial Key Lab of Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China State Key Lab of Rice Biology, Zhejiang University, Hangzhou, China Export Date: 19 January 2022 Correspondence Address: Chen, X.-X.; Institute of Insect Science, China; email: xxchen@zju.edu.cn AB - Polydnaviruses (PDVs) are obligatory symbionts of parasitoid wasps and play an important role in suppressing host immune defenses. Although PDV genes that inhibit host melanization are known in Microplitis bracovirus, the functional homologs in Cotesia bracoviruses remain unknown. Here, we find that Cotesia vestalis bracovirus (CvBV) can inhibit hemolymph melanization of its host, Plutella xylostella larvae, during the early stages of parasitization, and that overexpression of highly expressed CvBV genes reduced host phenoloxidase activity. Furthermore, CvBV-7-1 in particular reduced host phenoloxidase activity within 12 h, and the injection of anti-CvBV-7-1 antibody increased the melanization of parasitized host larvae. Further analyses showed that CvBV-7-1 and three homologs from other Cotesia bracoviruses possessed a C-terminal leucine/isoleucine-rich region and had a similar function in inhibiting melanization. Therefore, a new family of bracovirus genes was proposed and named as C-terminal Leucine/isoleucine-rich Protein (CLP). Ectopic expression of CvBV-7-1 in Drosophila hemocytes increased susceptibility to bacterial repression of melanization and reduced the melanotic encapsulation of parasitized D. melanogaster by the parasitoid Leptopilina boulardi. The formation rate of wasp pupae and the eclosion rate of C. vestalis were affected when the function of CvBV-7-1 was blocked. Our findings suggest that CLP genes from Cotesia bracoviruses encoded proteins that contain a C-terminal leucine/isoleucine-rich region and function as melanization inhibitors during the early stage of parasitization, which is important for successful parasitization. LA - English DB - MTMT ER - TY - JOUR AU - Bailetti, Alessandro A. AU - Negron-Pineiro, Lenny J. AU - Dhruva, Vishal AU - Harsh, Sneh AU - Lu, Sean AU - Bosula, Aisha AU - Bach, Erika A. TI - Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity JF - DISEASE MODELS & MECHANISMS J2 - DIS MODEL MECH VL - 12 PY - 2019 IS - 5 PG - 15 SN - 1754-8403 DO - 10.1242/dmm.038679 UR - https://m2.mtmt.hu/api/publication/30901165 ID - 30901165 N1 - Department of Biochemistry and Molecular Pharmacology, New York University, School of Medicine, New York, NY 10016, United States Helen L. and Martin S. Kimmel Center for Stem Cell Biology, New York University, School of Medicine, New York, NY 10016, United States Program in Epithelial Biology, Department of Dermatology, Stanford University, School of Medicine, Stanford, CA 94305, United States Cited By :5 Export Date: 19 January 2022 Correspondence Address: Bach, E.A.; Department of Biochemistry and Molecular Pharmacology, United States; email: erika.bach@nyu.edu AB - Myeloproliferative neoplasms (MPNs) are clonal hematopoietic disorders that cause excessive production of myeloid cells. Most MPN patients have a point mutation in JAK2 (JAK2(V617F)), which encodes a dominant-active kinase that constitutively triggers JAK/STAT signaling. In Drosophila, this pathway is simplified, with a singleJAK, Hopscotch (Hop), and a single STAT transcription factor, Stat92E. The hop(Tumorous-lethal) [hop(Tum)] allele encodes a dominant-active kinase that induces sustained Stat92E activation. Like MPN patients, hop(Tum) mutants have significantly more myeloid cells, which form invasive tumors. Through an unbiased genetic screen, we found that heterozygosity for Enhancer of Polycomb [E(Pc)], a component of the Tip60 lysine acetyltransferase complex (also known as KAT5 in humans), significantly increased tumor burden in hopTum animals. Hematopoietic depletion of E(Pc) or other Tip60 components in an otherwise wild-type background also induced blood cell tumors. The E(Pc) tumor phenotype was dependent on JAK/STAT activity, as concomitant depletion of hop or Stat92E inhibited tumor formation. Stat92E target genes were significantly upregulated in E(Pc)-mutant myeloid cells, indicating that loss of E(Pc) activates JAK STAT signaling. Neither the hop nor Stat92E gene was upregulated upon hematopoietic E(Pc) depletion, suggesting that the regulation of the JAK STAT pathway by E(Pc) is dependent on substrates other than histones. Indeed, E(Pc) depletion significantly increased expression of Hop protein in myeloid cells. This study indicates that E(Pc) works as a tumor suppressor by attenuating Hop protein expression and ultimately JAK STAT signaling. Since loss-of-function mutations in the human homologs of E(Pc) and Tip60 are frequently observed in cancer, our work could lead to new treatments for MPN patients.This article has an associated First Person interview with the first author of the paper. LA - English DB - MTMT ER - TY - JOUR AU - Banerjee, Utpal AU - Girard, Juliet R. AU - Goins, Lauren M. AU - Spratford, Carrie M. TI - Drosophila as a Genetic Model for Hematopoiesis JF - GENETICS J2 - GENETICS VL - 211 PY - 2019 IS - 2 SP - 367 EP - 417 PG - 51 SN - 0016-6731 DO - 10.1534/genetics.118.300223 UR - https://m2.mtmt.hu/api/publication/30510237 ID - 30510237 N1 - Export Date: 13 May 2019 CODEN: GENTA Correspondence Address: Banerjee, U.; University of California, 610 Charles E Young Drive East, United States; email: banerjee@mbi.ucla.edu AB - In this FlyBook chapter, we present a survey of the current literature on the development of the hematopoietic system in Drosophila. The Drosophila blood system consists entirely of cells that function in innate immunity, tissue integrity, wound healing, and various forms of stress response, and are therefore functionally similar to myeloid cells in mammals. The primary cell types are specialized for phagocytic, melanization, and encapsulation functions. As in mammalian systems, multiple sites of hematopoiesis are evident in Drosophila and the mechanisms involved in this process employ many of the same molecular strategies that exemplify blood development in humans. Drosophila blood progenitors respond to internal and external stress by coopting developmental pathways that involve both local and systemic signals. An important goal of these Drosophila studies is to develop the tools and mechanisms critical to further our understanding of human hematopoiesis during homeostasis and dysfunction. LA - English DB - MTMT ER - TY - JOUR AU - Cevik, Duygu AU - Acker, Meryl AU - Michalski, Camilla AU - Jacobs, J. Roger TI - Pericardin, a Drosophila collagen, facilitates accumulation of hemocytes at the heart JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 454 PY - 2019 IS - 1 SP - 52 EP - 65 PG - 14 SN - 0012-1606 DO - 10.1016/j.ydbio.2019.06.006 UR - https://m2.mtmt.hu/api/publication/30907377 ID - 30907377 N1 - Cited By :10 Export Date: 19 January 2022 CODEN: DEBIA Correspondence Address: Jacobs, J.R.; Department of Biology, 1280 Main St. West, Canada; email: jacobsr@mcmaster.ca AB - Hematopoietic cell lineages support organismal needs by responding to positional and systemic signals that balance proliferative and differentiation events. Drosophila provides an excellent genetic model to dissect these signals, where the activity of cues in the hemolymph or substrate can be traced to determination and differentiation events of well characterized hemocyte types. Plasmatocytes in third instar larvae increase in number in response to infection and in anticipation of metamorphosis. Here we characterize hemocyte clustering, proliferation and transdifferentiation on the heart or dorsal vessel. Hemocytes accumulate on the inner foldings of the heart basement membrane, where they move with heart contraction, and are in proximity to the heart ostia and pericardial nephrocytes. The numbers of hemocytes vary, but increase transiently before pupariation, and decrease by 4 h before pupa formation. During their accumulation at the heart, plasmatocytes can proliferate and can transdifferentiate into crystal cells. Serrate expressing cells as well as lamellocyte-like, Atilla expressing ensheathing cells are associated with some, but not all hemocyte clusters. Hemocyte aggregation is enhanced by the presence of a heart specific Collagen, Pericardin, but not the associated pericardial cells. The varied and transient number of hemocytes in the pericardial compartment suggests that this is not a hematopoietic hub, but a niche supporting differentiation and rapid dispersal in response to systemic signals. LA - English DB - MTMT ER - TY - JOUR AU - Cooper, Dustin AU - Wuebbolt, Caitlin AU - Heryanto, Christa AU - Eleftherianos, Ioannis TI - The prophenoloxidase system in Drosophila participates in the anti-nematode immune response JF - MOLECULAR IMMUNOLOGY J2 - MOL IMMUNOL VL - 109 PY - 2019 SP - 88 EP - 98 PG - 11 SN - 0161-5890 DO - 10.1016/j.molimm.2019.03.008 UR - https://m2.mtmt.hu/api/publication/30908691 ID - 30908691 N1 - Cited By :17 Export Date: 19 January 2022 CODEN: IMCHA Correspondence Address: Eleftherianos, I.; Department of Biological Sciences, 800 22ndStreet NW, United States; email: ioannise@gwu.edu AB - Drosophila melanogaster relies on an evolutionarily conserved innate immune system to protect itself from potentially deadly pathogens. One of the earliest pathways activated after injury or infection is the melanization pathway, which is responsible for synthesizing and depositing melanin at the site of injury, or onto invading microbes. Three genes, PPO1-3, encoding prophenoloxidase (PPO), an inactive precursor of phenoloxidase (PO), are responsible for the production of melanin after their activation via immune challenge. One pathogen capable of infecting D. melanogaster are entomopathogenic nematodes. Steinernema carpocapsae nematodes exist in a mutualistic relationship with Xenorhabdus nematophila bacteria and are an important biological control agent for controlling insect pests. The nematode-bacteria complex (symbiotic nematodes) can be separated, creating "axenic" nematodes, devoid of their associated bacteria, which are still capable of infecting and killing D. melanogaster. In order to investigate how the D. melanogaster melanization pathway contributes to the anti nematode immune response, symbiotic and axenic S. carpocapsae were used to study D. melanogaster survival, PPO gene expression, and activation of PPO to PO. Our research suggests that the expression of all three D. melanogaster PPO genes contributes to survival, however only PPO1 or PPO3 appear to be up-regulated during axenic or symbiotic nematode infection. Additionally, we present data suggesting that a complex regulatory system exists between PPOs, potentially allowing for the compensation of PPOs by one another. Further, we found that axenic nematode infection leads to higher levels of PO, suggesting that X. nematophila suppresses this activation. We also report for the first time the differentiation of lamellocytes, a specialized type of hemocytes in D. melanogaster, in response to symbiotic S. carpocapsae nematode infection. Our results suggest an important role played by the melanization pathway in response to nematode infection, and demonstrate how this response can be manipulated by S. carpocapsae nematodes and their mutualistic X. nematophila bacteria. LA - English DB - MTMT ER - TY - JOUR AU - Kim-Jo, Chami AU - Gatti, Jean-Luc AU - Poirie, Marylene TI - Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process JF - FRONTIERS IN PHYSIOLOGY J2 - FRONT PHYSIOL VL - 10 PY - 2019 PG - 8 SN - 1664-042X DO - 10.3389/fphys.2019.00603 UR - https://m2.mtmt.hu/api/publication/30907275 ID - 30907275 N1 - Cited By :19 Export Date: 19 January 2022 Correspondence Address: Poirié, M.; INRA, France; email: marylene.poirie@univ-cotedazur.fr AB - Host-parasitoid interactions are among the most studied interactions between invertebrates because of their fundamental interest - the evolution of original traits in parasitoids - and applied, parasitoids being widely used in biological control. Immunity, and in particular cellular immunity, is central in these interactions, the host encapsulation response being specific for large foreign bodies such as parasitoid eggs. Although already well studied in this species, recent data on Drosophila melanogaster have unquestionably improved knowledge of invertebrate cellular immunity. At the same time, the venomics of parasitoids has expanded, notably those of Drosophila. Here, we summarize and discuss these advances, with a focus on an emerging "time-dependent" view of interactions outcome at the intra- and interspecific level. We also present issues still in debate and prospects for study. Data on the Drosophila-parasitoid model paves the way to new concepts in insect immunity as well as parasitoid wasp strategies to overcome it. LA - English DB - MTMT ER - TY - JOUR AU - Lin, Yu-Hsien AU - Maaroufi, Houda Ouns AU - Ibrahim, Emad AU - Kucerova, Lucie AU - Zurovec, Michal TI - Expression of Human Mutant Huntingtin Protein in Drosophila Hemocytes Impairs Immune Responses JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 10 PY - 2019 PG - 15 SN - 1664-3224 DO - 10.3389/fimmu.2019.02405 UR - https://m2.mtmt.hu/api/publication/30961322 ID - 30961322 N1 - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czech Republic Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic Export Date: 3 February 2020 Correspondence Address: Lin, Y.-H.; Biology Centre of the Czech Academy of Sciences, Institute of EntomologyCzech Republic; email: r99632012@gmail.com AB - The pathogenic effect of mutant HTT (mHTT) which causes Huntington disease (HD) are not restricted to nervous system. Such phenotypes include aberrant immune responses observed in the HD models. However, it is still unclear how this immune dysregulation influences the innate immune response against pathogenic infection. In the present study, we used transgenic Drosophila melanogaster expressing mutant HTT protein (mHTT) with hemocyte-specific drivers and examined the immune responses and hemocyte function. We found that mHTT expression in the hemocytes did not affect fly viability, but the numbers of circulating hemocytes were significantly decreased. Consequently, we observed that the expression of mHTT in the hemocytes compromised the immune responses including clot formation and encapsulation which lead to the increased susceptibility to entomopathogenic nematode and parasitoid wasp infections. In addition, mHTT expression in Drosophila macrophage-like S2 cells in vitro reduced ATP levels, phagocytic activity and the induction of antimicrobial peptides. Further effects observed in mHTT-expressing cells included the altered production of cytokines and activation of JAK/STAT signaling. The present study shows that the expression of mHTT in Drosophila hemocytes causes deficient cellular and humoral immune responses against invading pathogens. Our findings provide the insight into the pathogenic effects of mHTT in the immune cells. LA - English DB - MTMT ER - TY - JOUR AU - Lin, Zhe AU - Wang, Rui-Juan AU - Cheng, Yang AU - Du, Jie AU - Volovych, Olga AU - Han, Li-Bin AU - Li, Jian-Cheng AU - Hu, Yang AU - Lu, Zi-Yun AU - Lu, Zhiqiang AU - Zou, Zhen TI - Insights into the venom protein components of Microplitis mediator, an endoparasitoid wasp JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 105 PY - 2019 SP - 33 EP - 42 PG - 10 SN - 0965-1748 DO - 10.1016/j.ibmb.2018.12.013 UR - https://m2.mtmt.hu/api/publication/30518197 ID - 30518197 N1 - State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China Department of Entomology, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China Institute of Plant Protection of Hebei Academy of Agriculture and Forestry Sciences, Baoding, China University of Chinese Academy of Sciences, Beijing, China Cited By :15 Export Date: 19 January 2022 CODEN: IBMBE Correspondence Address: Zou, Z.; State Key Laboratory of Integrated Management of Pest Insects and Rodents, China; email: zouzhen@ioz.ac.cn AB - Endoparasitoid wasps deliver a variety of maternal factors, such as venom proteins, viruses, and virus-like particles, from their venom and calyx fluid into hosts and thereby regulate the hosts' immune response, metabolism and development. The endoparasitoid, Microplitis mediator, is used as an important biological agent for controlling the devastating pest Helicoverpa armigera. In this study, using an integrated transcriptomic and proteomic analysis approach, we identified 75 putative venom proteins in M. mediator. The identified venom components were consistent with other known parasitoid wasps' venom proteins, including metalloproteases, serine protease inhibitors, and glycoside hydrolase family 18 enzymes. The metalloprotease and serpin family showed extensive gene duplications in venom apparatus. Isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative proteomics revealed 521 proteins that were differentially expressed at 6 h and 24 h post-parasitism, including 10 wasp venom proteins that were released into the host hemolymph. Further analysis indicated that 511 differentially expressed proteins (DEP) from the host are primarily involved in the immune response, material metabolism, and extracellular matrix receptor interaction. Taken together, our results on parasitoid wasp venoms have the potential to enhance the application of endoparasitoid wasps for controlling insect pest. LA - English DB - MTMT ER - TY - JOUR AU - Melcarne, Claudia AU - Ramond, Elodie AU - Dudzic, Jan AU - Bretscher, Andrew AU - Kurucz, Judit Éva AU - Andó, István AU - Lemaitre, Bruno TI - Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster JF - FEBS JOURNAL J2 - FEBS J VL - 286 PY - 2019 IS - 14 SP - 2670 EP - 2691 PG - 22 SN - 1742-464X DO - 10.1111/febs.14857 UR - https://m2.mtmt.hu/api/publication/30641961 ID - 30641961 N1 - Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary Cited By :13 Export Date: 19 January 2022 CODEN: FJEOA Correspondence Address: Melcarne, C.; Global Health Institute, Switzerland; email: claudia.melcarne@epfl.ch LA - English DB - MTMT ER - TY - JOUR AU - Mihajlovic, Zorana AU - Tanasic, Dajana AU - Bajgar, Adam AU - Perez-Gomez, Raquel AU - Steffal, Pavel AU - Krejci, Alena TI - Lime is a new protein linking immunity and metabolism in Drosophila JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 452 PY - 2019 IS - 2 SP - 83 EP - 94 PG - 12 SN - 0012-1606 DO - 10.1016/j.ydbio.2019.05.005 UR - https://m2.mtmt.hu/api/publication/30913672 ID - 30913672 N1 - University of South Bohemia, Faculty of Science, Ceske Budejovice, Czech Republic Czech Academy of Sciences, Biology Centre, Institute of Entomology, Ceske Budejovice, Czech Republic Cited By :2 Export Date: 14 February 2021 CODEN: DEBIA Correspondence Address: Krejci, A.; DT - Department of Cell and Molecular Biology, Germany; email: akrejci@prf.jcu.cz AB - The proliferation, differentiation and function of immune cells in vertebrates, as well as in the invertebrates, is regulated by distinct signalling pathways and crosstalk with systemic and cellular metabolism. We have identified the Lime gene (Linking Immunity and Metabolism, CG18446) as one such connecting factor, linking hemocyte development with systemic metabolism in Drosophila. Lime is expressed in larval plasmatocytes and the fat body and regulates immune cell type and number by influencing the size of hemocyte progenitor populations in the lymph gland and in circulation. Lime mutant larvae exhibit low levels of glycogen and trehalose energy reserves and they develop low number of hemocytes. The low number of hemocytes in Lime mutants can be rescued by Lime overexpression in the fat body. It is well known that immune cell metabolism is tightly regulated with the progress of infection and it must be supported by systemic metabolic changes. Here we demonstrate that Lime mutants fails to induce such systemic metabolic changes essential for the larval immune response. Indeed, Lime mutants are not able to sustain high numbers of circulating hemocytes and are compromised in the number of lamellocytes produced during immune system challenge, using a parasitic wasp infection model. We therefore propose the Lime gene as a novel functional link between systemic metabolism and Drosophila immunity. LA - English DB - MTMT ER - TY - JOUR AU - Salminen, Tiina S. AU - Cannino, Giuseppe AU - Oliveira, Marcos T. AU - Lillsunde, Paivi AU - Jacobs, Howard T. AU - Kaguni, Laurie S. TI - Lethal Interaction of Nuclear and Mitochondrial Genotypes in Drosophila melanogaster JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 9 PY - 2019 IS - 7 SP - 2225 EP - 2234 PG - 10 SN - 2160-1836 DO - 10.1534/g3.119.400315 UR - https://m2.mtmt.hu/api/publication/30907260 ID - 30907260 N1 - Faculty of Medicine and Health Technology, University of TampereFI-331014, Finland Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, SP 14884-900, Brazil Institute of Biotechnology, University of Helsinki, Helsinki, FI-00014, Finland Department of Biochemistry and Molecular Biology, Center for Mitochondrial Science and Medicine, Michigan State University, 603Wilson Rd., East Lansing, MI 48824, United States Cited By :3 Export Date: 19 January 2022 Correspondence Address: Jacobs, H.T.; Faculty of Medicine and Health Technology, Finland; email: howard.jacobs@tuni.fi AB - Drosophila melanogaster, like most animal species, displays considerable genetic variation in both nuclear and mitochondrial DNA (mtDNA). Here we tested whether any of four natural mtDNA variants was able to modify the effect of the phenotypically mild, nuclear tko(25t) mutation, affecting mitochondrial protein synthesis. When combined with tko(25t), the mtDNA from wild strain KSA2 produced pupal lethality, accompanied by the presence of melanotic nodules in L3 larvae. KSA2 mtDNA, which carries a substitution at a conserved residue of cytochrome b that is predicted to be involved in subunit interactions within respiratory complex III, conferred drastically decreased respiratory capacity and complex III activity in the tko(25t) but not a wild-type nuclear background. The complex III inhibitor antimycin A was able to phenocopy effects of the tko(25t) mutation in the KSA2 mtDNA background. This is the first report of a lethal, nuclear-mitochondrial interaction within a metazoan species, representing a paradigm for understanding genetic interactions between nuclear and mitochondrial genotype relevant to human health and disease. LA - English DB - MTMT ER - TY - JOUR AU - Schmid, Martin R. AU - Dziedziech, Alexis AU - Arefin, Badrul AU - Kienzle, Thomas AU - Wang, Zhi AU - Akhter, Munira AU - Berka, Jakub AU - Theopold, Ulrich TI - Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 109 PY - 2019 SP - 63 EP - 71 PG - 9 SN - 0965-1748 DO - 10.1016/j.ibmb.2019.04.007 UR - https://m2.mtmt.hu/api/publication/30901163 ID - 30901163 N1 - Cited By :12 Export Date: 19 January 2022 CODEN: IBMBE Correspondence Address: Theopold, U.; Department of Molecular Biosciences, Svante Arrhenius väg 20c, Sweden; email: uli.theopold@su.se AB - In most insects, hemolymph coagulation, which is analogous to mammalian blood clotting, involves close collaboration between humoral and cellular components. To gain insights into the secretion of cellular clotting factors, we created tagged versions of three different clotting factors. Our focus was on factors which are released in a non-classical manner and to characterize them in comparison to a protein that is classically released, namely Glutactin (Glt). Transglutaminase-A (Tg) and Prophenoloxidase 2 (PPO2), both of which lack signal peptide sequences, have been previously demonstrated to be released from plasmatocytes and crystal cells (CCs) respectively, the two hemocyte classes in naive larvae. We found that at the molecular level, Tg secretion resembles the release of tissue transglutaminase in mammals. Specifically, Drosophila Tg is associated with vesicular membranes and remains membrane-bound after release, in contrast to Glt, which we found localizes to a different class of vesicles and is integrated into clot fibers. PPO2 on the other hand, is set free from CCs through cytolysis. We confirm that PPO2 is a central component of the cytosolic crystals and find that the distribution of PPO2 appears to vary across crystals and cells. We propose a tentative scheme for the secretory events during early and late hemolymph coagulation. LA - English DB - MTMT ER - TY - JOUR AU - Valanne, Susanna AU - Salminen, Tiina S. AU - Jarvela-Stolting, Mirva AU - Vesala, Laura AU - Ramet, Mika TI - Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 15 PY - 2019 IS - 1 PG - 28 SN - 1553-7366 DO - 10.1371/journal.ppat.1007504 UR - https://m2.mtmt.hu/api/publication/30509856 ID - 30509856 N1 - Laboratory of Experimental Immunology, BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland PEDEGO Research Unit, and Medical Research Center Oulu, University of Oulu, Department of Children and Adolescents, Oulu University Hospital, Oulu, Finland Department of Pediatrics, Tampere University Hospital, Tampere, Finland Cited By :18 Export Date: 19 January 2022 Correspondence Address: Rämet, M.; Laboratory of Experimental Immunology, Finland; email: mika.ramet@uta.fi AB - Non-coding RNAs have important roles in regulating physiology, including immunity. Here, we performed transcriptome profiling of immune-responsive genes in Drosophila melanogaster during a Gram-positive bacterial infection, concentrating on long non-coding RNA (lncRNA) genes. The gene most highly induced by a Micrococcus luteus infection was CR44404, named Induced by Infection (lincRNA-IBIN). lincRNA-IBIN is induced by both Gram-positive and Gram-negative bacteria in Drosophila adults and parasitoid wasp Leptopilina boulardi in Drosophila larvae, as well as by the activation of the Toll or the Imd pathway in unchallenged flies. We show that upon infection, lincRNA-IBIN is expressed in the fat body, in hemocytes and in the gut, and its expression is regulated by NF-B signaling and the chromatin modeling brahma complex. In the fat body, overexpression of lincRNA-IBIN affected the expression of Toll pathway -mediated genes. Notably, overexpression of lincRNA-IBIN in unchallenged flies elevated sugar levels in the hemolymph by enhancing the expression of genes important for glucose retrieval. These data show that lncRNA genes play a role in Drosophila immunity and indicate that lincRNA-IBIN acts as a link between innate immune responses and metabolism. LA - English DB - MTMT ER - TY - JOUR AU - Varga, Gergely István AU - Csordás, Gábor AU - Cinege, Gyöngyi Ilona AU - Jankovics, Ferenc AU - Sinka, Rita AU - Kurucz, Judit Éva AU - Andó, István AU - Honti, Viktor TI - Headcase is a Repressor of Lamellocyte Fate in Drosophila melanogaster JF - GENES J2 - GENES-BASEL VL - 10 PY - 2019 IS - 3 PG - 17 SN - 2073-4425 DO - 10.3390/genes10030173 UR - https://m2.mtmt.hu/api/publication/30585796 ID - 30585796 N1 - Laboratory of Immunology, Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Laboratory of Drosophila Germ Cell Differentiation, Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Department of Genetics, Faculty of Science and Informatics, University of Szeged, Szeged, 6726, Hungary Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, 50931, Germany Cited By :3 Export Date: 19 January 2022 Correspondence Address: Andó, I.; Laboratory of Immunology, Hungary; email: ando.istvan@brc.mta.hu AB - Due to the evolutionary conservation of the regulation of hematopoiesis, Drosophila provides an excellent model organism to study blood cell differentiation and hematopoietic stem cell (HSC) maintenance. The larvae of Drosophila melanogaster respond to immune induction with the production of special effector blood cells, the lamellocytes, which encapsulate and subsequently kill the invader. Lamellocytes differentiate as a result of a concerted action of all three hematopoietic compartments of the larva: the lymph gland, the circulating hemocytes, and the sessile tissue. Within the lymph gland, the communication of the functional zones, the maintenance of HSC fate, and the differentiation of effector blood cells are regulated by a complex network of signaling pathways. Applying gene conversion, mutational analysis, and a candidate based genetic interaction screen, we investigated the role of Headcase (Hdc), the homolog of the tumor suppressor HECA in the hematopoiesis of Drosophila. We found that naive loss-of-function hdc mutant larvae produce lamellocytes, showing that Hdc has a repressive role in effector blood cell differentiation. We demonstrate that hdc genetically interacts with the Hedgehog and the Decapentaplegic pathways in the hematopoietic niche of the lymph gland. By adding further details to the model of blood cell fate regulation in the lymph gland of the larva, our findings contribute to the better understanding of HSC maintenance. LA - English DB - MTMT ER - TY - JOUR AU - Wan, Bin AU - Goguet, Emilie AU - Ravallec, Marc AU - Pierre, Olivier AU - Lemauf, Severine AU - Volkoff, Anne-Nathalie AU - Gatti, Jean-Luc AU - Poirie, Marylone TI - Venom Atypical Extracellular Vesicles as Interspecies Vehicles of Virulence Factors Involved in Host Specificity: The Case of a Drosophila Parasitoid Wasp JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 10 PY - 2019 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2019.01688 UR - https://m2.mtmt.hu/api/publication/30907274 ID - 30907274 N1 - Université Côte d'Azur, CNRS, ISA, Sophia Antipolis, France Univ. Montpellier, UMR 1333 'Microorganism and Insect Diversity, Genomes and Interactions' (DGIMI), Montpellier, France State Key Laboratory of Rice Biology and Ministry of Agricultural and Rural Affairs, Institute of Insect Sciences, Zhejiang University, Hangzhou, China Henry M. Jackson Foundation at the Uniformed Services University of the Health Sciences, Department of Microbiology and Immunology, Bethesda, MD, United States Cited By :19 Export Date: 19 January 2022 Correspondence Address: Poirié, M.; Université Côte d'Azur, France; email: marylene.poirie@univ-cotedazur.fr AB - Endoparasitoid wasps, which lay eggs inside the bodies of other insects, use various strategies to protect their offspring from the host immune response. The hymenopteran species of the genus Leptopilina, parasites of Drosophila, rely on the injection of a venom which contains proteins and peculiar vesicles (hereafter venosomes). We show here that the injection of purified L. boulardi venosomes is sufficient to impair the function of the Drosophila melanogaster lamellocytes, a hemocyte type specialized in the defense against wasp eggs, and thus the parasitic success of the wasp. These venosomes seem to have a unique extracellular biogenesis in the wasp venom apparatus where they acquire specific secreted proteins/virulence factors and act as a transport system to deliver these compounds into host lamellocytes. The level of venosomes entry into lamellocytes of different Drosophila species was correlated with the rate of parasitism success of the wasp, suggesting that this venosome-cell interaction may represent a new evolutionary level of host-parasitoid specificity. LA - English DB - MTMT ER - TY - JOUR AU - Bazzi, Wael AU - Cattenoz, Pierre B AU - Delaporte, Claude AU - Dasari, Vasanthi AU - Sakr, Rosy AU - Yuasa, Yoshihiro AU - Giangrande, Angela TI - Embryonic hematopoiesis modulates the inflammatory response and larval hematopoiesis in Drosophila JF - ELIFE J2 - ELIFE VL - 7 PY - 2018 PG - 30 SN - 2050-084X DO - 10.7554/eLife.34890.001 UR - https://m2.mtmt.hu/api/publication/27602855 ID - 27602855 AB - Recent lineage tracing analyses have significantly improved our understanding of immune system development and highlighted the importance of the different hematopoietic waves. The current challenge is to understand whether these waves interact and whether this affects the function of the immune system. Here we report a molecular pathway regulating the immune response and involving the communication between embryonic and larval hematopoietic waves in Drosophila. Down-regulating the transcription factor Gcm specific to embryonic hematopoiesis enhances the larval phenotypes induced by over-expressing the pro-inflammatory Jak/Stat pathway or by wasp infestation. Gcm works by modulating the transduction of the Upd cytokines to the site of larval hematopoiesis and hence the response to chronic Jak/Stat overexpression and acute wasp infestation immune challenges. Thus, homeostatic interactions control the function of the immune system in physiology and pathology. Our data also indicate that a transiently expressed developmental pathway has a long-lasting effect on the immune response. © Bazzi et al. LA - English DB - MTMT ER - TY - JOUR AU - Boulet, Manon AU - Miller, Marion AU - Vandel, Laurence AU - Waltzer, Lucas TI - From Drosophila Blood Cells to Human Leukemia JF - ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY J2 - ADV EXP MED BIOL VL - 1076 PY - 2018 SP - 195 EP - 214 PG - 20 SN - 0065-2598 DO - 10.1007/978-981-13-0529-0_11 UR - https://m2.mtmt.hu/api/publication/30510499 ID - 30510499 N1 - Cited By :8 Export Date: 15 February 2024 CODEN: AEMBA Correspondence Address: Waltzer, L.; Université Clermont Auvergne, France; email: lucas.waltzer@uca.fr AB - The hematopoietic system plays a critical role in establishing the proper response against invading pathogens or in removing cancerous cells. Furthermore, deregulations of the hematopoietic differentiation program are at the origin of numerous diseases including leukemia. Importantly, many aspects of blood cell development have been conserved from human to Drosophila. Hence, Drosophila has emerged as a potent genetic model to study blood cell development and leukemia in vivo. In this chapter, we give a brief overview of the Drosophila hematopoietic system, and we provide a protocol for the dissection and the immunostaining of the larval lymph gland, the most studied hematopoietic organ in Drosophila. We then focus on the various paradigms that have been used in fly to investigate how conserved genes implicated in leukemogenesis control blood cell development. Specific examples of Drosophila models for leukemia are presented, with particular attention to the most translational ones. Finally, we discuss some limitations and potential improvements of Drosophila models for studying blood cell cancer. LA - English DB - MTMT ER - TY - JOUR AU - Hao, Yangguang AU - Yu, Shichao AU - Luo, Fangzhou AU - Jin, Li Hua TI - Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila JF - CELL COMMUNICATION AND SIGNALING J2 - CELL COMM SIGN VL - 16 PY - 2018 PG - 20 SN - 1478-811X DO - 10.1186/s12964-018-0305-3 UR - https://m2.mtmt.hu/api/publication/30510502 ID - 30510502 N1 - Department of Genetics, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China Department of Translational Medicine Research Center, Shenyang Medical College, Shenyang, 110034, China Cited By :7 Export Date: 19 January 2022 Correspondence Address: Jin, L.H.; Department of Genetics, China; email: lhjin@nefu.edu.cn AB - Background: The regulatory mechanisms of hematopoiesis and cellular immunity show a high degree of similarity between insects and mammals, and Drosophila has become a good model for investigating cellular immune responses. Jumeau (Jumu) is a member of the winged-helix/forkhead (FKH) transcription factor family and is required for Drosophila development. Adult jumu mutant flies show defective hemocyte phagocytosis and a weaker defense capability against pathogen infection. Here, we further investigated the role of jumu in the regulation of larval hemocyte development and phagocytosis. LA - English DB - MTMT ER - TY - JOUR AU - Iacovone, Alessia AU - Ris, Nicolas AU - Poirie, Marylene AU - Gatti, Jean-Luc TI - Time-course analysis of Drosophila suzukii interaction with endoparasitoid wasps evidences a delayed encapsulation response compared to D-melanogaster JF - PLOS ONE J2 - PLOS ONE VL - 13 PY - 2018 IS - 8 PG - 16 SN - 1932-6203 DO - 10.1371/journal.pone.0201573 UR - https://m2.mtmt.hu/api/publication/30514422 ID - 30514422 AB - Drosophila suzukii (the spotted-wing Drosophila) appears to be unsuitable for the development of most Drosophila larval endoparasitoids, be they sympatric or not. Here, we questioned the physiological bases of this widespread failure by characterizing the interactions between D. suzukii and various parasitoid species (Asobara japonica, Leptopiiina boulardi, Leptopilina heterotoma and Leptopilina victoriae) and comparing them with those observed with D. meianogaster, a rather appropriate host. All parasitoids were able to oviposit in L1 and L2 larval stages of both hosts but their propensity to parasitize was higher on D. meianogaster. A. japonica and, to a much lesser extent, L. heterotoma, were the two species able to successfully develop in D. suzukii, the failure of the parasitism resulting either in the parasitoid encapsulation (notably with L. heterotoma) or the host and parasitoid deaths (especially with L. boulardi and L. victoriae). Compared to D. meianogaster, encapsulation in D. suzukii was strongly delayed and led, if successful, to the production of much larger capsules in surviving flies and, in the event of failure, to the death of both partners because of an uncontrolled melanization. The results thus revealed a different timing of the immune response to parasitoids in D. suzukii compared to D. melanogaster with a lose-lose outcome for parasitoids (generally unsuccessful development) and hosts (high mortality and possible reduction of the fitness of survivors). Finally, these results might suggest that some European endoparasitoids of Drosophila interact with this pest in the field in an unmeasurable way, since they kill their host without reproductive success. LA - English DB - MTMT ER - TY - JOUR AU - Lin, Zhe AU - Cheng, Yang AU - Wang, Rui-Juan AU - Du, Jie AU - Volovych, Olga AU - Li, Jian-Cheng AU - Hu, Yang AU - Lu, Zi-Yun AU - Lu, Zhiqiang AU - Zou, Zhen TI - A Metalloprotease Homolog Venom Protein From a Parasitoid Wasp Suppresses the Toll Pathway in Host Hemocytes JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 9 PY - 2018 PG - 15 SN - 1664-3224 DO - 10.3389/fimmu.2018.02301 UR - https://m2.mtmt.hu/api/publication/30510505 ID - 30510505 AB - Parasitoid wasps depend on a variety of maternal virulence factors to ensure successful parasitism. Encapsulation response carried out by host hemocytes is one of the major host immune responses toward limiting endoparasitoid wasp offspring production. We found that VRF1, a metalloprotease homolog venom protein identified from the endoparasitoid wasp, Microplitis mediator, could modulate egg encapsulation in its host, the cotton bollworm, Helicoverpa armigera. Here, we show that the VRF1 proenzyme is cleaved after parasitism, and that the C-terminal fragment containing the catalytic domain enters host hemocytes 6 h post-parasitism. Furthermore, using yeast two-hybrid and pull-down assays, VRF1 is shown to interact with the H. armigera NF-kappa B factor, Dorsal. We also show that overexpressed of VRF1 in an H. armigera cell line cleaved Dorsal in vivo. Taken together, our results have revealed a novel mechanism by which a component of endoparasitoid wasp venom interferes with the Toll signaling pathway in the host hemocytes. LA - English DB - MTMT ER - TY - JOUR AU - Mandal, L. AU - Ghosh, S. AU - Mandal, S. TI - Detecting proliferation of adult hemocytes in Drosophila by BrdU incorporation [version 1; referees: 2 approved] JF - WELLCOME OPEN RESEARCH J2 - WELLCOME OPEN RESEARCH VL - 3 PY - 2018 SN - 2398-502X DO - 10.12688/wellcomeopenres.14560.1 UR - https://m2.mtmt.hu/api/publication/31149601 ID - 31149601 N1 - Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-Mohali, Manauli, Punjab, 140306, India Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-Mohali, Manauli, Punjab, 140306, India Export Date: 31 January 2020 Correspondence Address: Mandal, L.; Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-MohaliIndia; email: lolitika@iisermohali.ac.in Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-Mohali, Manauli, Punjab, 140306, India Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-Mohali, Manauli, Punjab, 140306, India Cited By :1 Export Date: 12 February 2021 Correspondence Address: Mandal, L.; Developmental Genetics Laboratory, India; email: lolitika@iisermohali.ac.in AB - Drosophila and mammalian hematopoiesis share several similarities that ranges from phases to the battery of transcription factors and signaling molecules that execute this process. These resounding similarities along with the rich genetic tools available in fruitfly makes it a popular invertebrate model to study blood cell development both during normal and aberrant conditions. The larval system is the most extensively studied to date. Several studies have shown that these hemocytes just like mammalian counterpart proliferate and get routinely regenerated upon infection. However, employing the same protocol it was concluded that blood cell proliferation although abundant in larval stages is absent in adult fruitfly. The current protocol describes the strategies that can be employed to document the hemocyte proliferation in adulthood. The fact that a subset of blood cells tucked away in the hematopoietic hub are not locked in senescence, rather they still harbour the proliferative capacity to tide over challenges was successfully demonstrated by this method. Although we have adopted bacterial infection as a bait to evoke this proliferative capacity of the hemocytes, we envision that it can also efficiently characterize the proliferative responses of hemocytes in tumorigenic conditions as well as scenarios of environmental and metabolic stresses during adulthood. © 2018 Ghosh S et al. LA - English DB - MTMT ER - TY - JOUR AU - Melillo, Daniela AU - Marino, Rita AU - Italiani, Paola AU - Boraschi, Diana TI - Innate Immune Memory in Invertebrate Metazoans: A Critical Appraisal JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 9 PY - 2018 PG - 17 SN - 1664-3224 DO - 10.3389/fimmu.2018.01915 UR - https://m2.mtmt.hu/api/publication/30518205 ID - 30518205 AB - The ability of developing immunological memory, a characteristic feature of adaptive immunity, is clearly present also in innate immune responses. In fact, it is well known that plants and invertebrate metazoans, which only have an innate immune system, can mount a faster and more effective response upon re-exposure to a stimulus. Evidence of immune memory in invertebrates comes from studies in infection immunity, natural transplantation immunity, individual, and transgenerational immune priming. These studies strongly suggest that environment and lifestyle take part in the development of immunological memory. However, in several instances the formal correlation between the phenomenon of immune memory and molecular and functional immune parameters is stillmissing. In this review, we have critically examined the cellular and humoral aspects of the invertebrate immune memory responses. In particular, we have focused our analysis on studies that have addressed immune memory in the most restrictive meaning of the term, i.e., the response to a challenge of a quiescent immune system that has been primed in the past. These studies highlight the central role of an increase in the number of immune cells and of their epigenetic re-programming in the establishment of sensu stricto immune memory in invertebrates. LA - English DB - MTMT ER - TY - JOUR AU - Ozturk, Gurhan AU - Cakici, Ozlem AU - Arikan, Huseyin TI - Morphological characterization of hemocyte types in some species belonging to Tettigoniidae and Pamphagidae (Insecta: Orthoptera) JF - TURKISH JOURNAL OF ZOOLOGY J2 - TURK J ZOOL VL - 42 PY - 2018 IS - 3 SP - 340 EP - 345 PG - 6 SN - 1300-0179 DO - 10.3906/zoo-1711-11 UR - https://m2.mtmt.hu/api/publication/27524974 ID - 27524974 LA - English DB - MTMT ER - TY - JOUR AU - Tokusumi, Yumiko AU - Tokusumi, Tsuyoshi AU - Schulz, Robert A TI - Mechanical stress to Drosophila larvae stimulates a cellular immune response through the JAK/STAT signaling pathway JF - BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS J2 - BIOCHEM BIOPH RES CO VL - 502 PY - 2018 IS - 3 SP - 415 EP - 421 PG - 7 SN - 0006-291X DO - 10.1016/j.bbrc.2018.05.192 UR - https://m2.mtmt.hu/api/publication/27602854 ID - 27602854 N1 - Cited By :10 Export Date: 19 January 2022 CODEN: BBRCA Correspondence Address: Schulz, R.A.; Department of Biological Sciences, United States; email: rschulz@nd.edu LA - English DB - MTMT ER - TY - JOUR AU - Valanne, Susanna AU - Vesala, Laura AU - Ramet, Mika TI - Commentary: Drosophila GATA Factor Serpent Establishes Phagocytic Ability of Embryonic Macrophages JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 9 PY - 2018 PG - 3 SN - 1664-3224 DO - 10.3389/fimmu.2018.01582 UR - https://m2.mtmt.hu/api/publication/27602652 ID - 27602652 LA - English DB - MTMT ER - TY - JOUR AU - Arefin, Badrul AU - Kunc, Martin AU - Krautz, Robert AU - Theopold, Ulrich TI - The Immune Phenotype of Three Drosophila Leukemia Models JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 7 PY - 2017 IS - 7 SP - 2139 EP - 2149 PG - 11 SN - 2160-1836 DO - 10.1534/g3.117.039487 UR - https://m2.mtmt.hu/api/publication/26714387 ID - 26714387 N1 - Megjegyzés-26674063 N1 Funding details: VR-2010-5988, VR, Vetenskapsrådet N1 Funding text: We thank Traimate Sangsuwan, Stockholm University, for helping with the extraction of mRNA of uninfected samples. Our work is supported by the Swedish Research Council (VR-2010-5988 and VR 2016-04077), the Swedish Foundation for International Cooperation in Research and Higher Education (IG2011-2042), the Knut and Alice Wallenberg Foundation (KAW2012.0058), and the Swedish Cancer Foundation (CAN 2010/553). Megjegyzés-26674034 N1 Funding details: VR-2010-5988, VR, Vetenskapsrådet N1 Funding text: We thank Traimate Sangsuwan, Stockholm University, for helping with the extraction of mRNA of uninfected samples. Our work is supported by the Swedish Research Council (VR-2010-5988 and VR 2016-04077), the Swedish Foundation for International Cooperation in Research and Higher Education (IG2011-2042), the Knut and Alice Wallenberg Foundation (KAW2012.0058), and the Swedish Cancer Foundation (CAN 2010/553). LA - English DB - MTMT ER - TY - JOUR AU - Armitage, S.A.O. AU - Kurtz, J. AU - Brites, D. AU - Dong, Y. AU - Pasquier, L.D. AU - Wang, H.-C. TI - Dscam1 in pancrustacean immunity: Current status and a look to the future JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 8 PY - 2017 SN - 1664-3224 DO - 10.3389/fimmu.2017.00662 UR - https://m2.mtmt.hu/api/publication/31149602 ID - 31149602 N1 - Institute for Evolution and Biodiversity, University of Münster, Münster, Germany Tuberculosis Research Unit, Swiss Tropical and Public Health Institute, Basel, Switzerland Zoological Institute, University of Basel, Basel, Switzerland Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, John Hopkins University, Baltimore, MD, United States Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan Cited By :12 Export Date: 31 January 2020 Correspondence Address: Armitage, S.A.O.; Institute for Evolution and Biodiversity, University of MünsterGermany; email: sophie.armitage@uni-muenster.de AB - The Down syndrome cell adhesion molecule 1 (Dscam1) gene is an extraordinary example of diversity: by combining alternatively spliced exons, thousands of isoforms can be produced from just one gene. So far, such diversity in this gene has only been found in insects and crustaceans, and its essential part in neural wiring has been well-characterized for Drosophila melanogaster. Ten years ago evidence from D. melanogaster showed that the Dscam1 gene is involved in insect immune defense and work on Anopheles gambiae indicated that it is a hypervariable immune receptor. These exciting findings showed that via processes of somatic diversification insects have the possibility to produce unexpected immune molecule diversity, and it was hypothesized that Dscam1 could provide the mechanistic underpinnings of specific immune responses. Since these first publications the quest to understand the function of this gene has uncovered fascinating insights from insects and crustaceans. However, we are still far from a complete understanding of how Dscam1 functions in relation to parasites and pathogens and its full relevance for the immune system. In this Hypothesis and Theory article, we first briefly introduce Dscam1 and what we know so far about how it might function in immunity. By focusing on seven questions, we then share our sometimes contrasting thoughts on what the evidence tells us so far, what essential experiments remain to be done, and the future prospects, with the aim to provide a multiangled view on what this fascinating gene has to do with immune defense. © 2017 Armitage, Kurtz, Brites, Dong, Du Pasquier and Wang. LA - English DB - MTMT ER - TY - JOUR AU - Louradour, Isabelle AU - Sharma, Anurag AU - Morin-Poulard, Ismael AU - Letourneau, Manon AU - Vincent, Alain AU - Crozatier, Michele AU - Vanzo, Nathalie TI - Reactive oxygen species-dependent Toll/NF-kappa B activation in the Drosophila hematopoietic niche confers resistance to wasp parasitism JF - ELIFE J2 - ELIFE VL - 6 PY - 2017 PG - 22 SN - 2050-084X DO - 10.7554/eLife.25496 UR - https://m2.mtmt.hu/api/publication/27260897 ID - 27260897 N1 - Centre de Biologie du Développement, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France National Institutes of Health, Bethesda, United States Department of Biomedical Sciences, NU Centre for Science Education and Research, Nitte University, Mangalore, India Hubrecht Institute, Utrecht, Netherlands Cited By :35 Export Date: 19 January 2022 Correspondence Address: Crozatier, M.; Centre de Biologie du Développement, France; email: michele.crozatier-borde@univ-tlse3.fr LA - English DB - MTMT ER - TY - JOUR AU - Terriente-Felix, Ana AU - Perez, Lidia AU - Bray, Sarah J AU - Nebreda, Angel R AU - Milan, Marco TI - A Drosophila model of myeloproliferative neoplasm reveals a feed-forward loop in the JAK pathway mediated by p38 MAPK signalling JF - DISEASE MODELS & MECHANISMS J2 - DIS MODEL MECH VL - 10 PY - 2017 IS - 4 SP - 399 EP - 407 PG - 9 SN - 1754-8403 DO - 10.1242/dmm.028118 UR - https://m2.mtmt.hu/api/publication/26698808 ID - 26698808 N1 - Cited By :11 Export Date: 30 June 2022 LA - English DB - MTMT ER - TY - JOUR AU - Tokusumi, Yumiko AU - Tokusumi, Tsuyoshi AU - Schulz, Robert A TI - The nociception genes painless and Piezo are required for the cellular immune response of Drosophila larvae to wasp parasitization JF - BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS J2 - BIOCHEM BIOPH RES CO VL - 486 PY - 2017 IS - 4 SP - 893 EP - 897 PG - 5 SN - 0006-291X DO - 10.1016/j.bbrc.2017.03.116 UR - https://m2.mtmt.hu/api/publication/26698807 ID - 26698807 N1 - Megjegyzés-26674061 N1 Funding details: AI121985, NIH, National Institutes of Health N1 Funding text: We thank A. Patapoutian, T. Schlenke, and stock centers for Drosophila strains and wasps. This work was supported by a grant to R.A.S from the National Institutes of Health (AI121985). Megjegyzés-26674023 N1 Funding details: AI121985, NIH, National Institutes of Health N1 Funding text: We thank A. Patapoutian, T. Schlenke, and stock centers for Drosophila strains and wasps. This work was supported by a grant to R.A.S from the National Institutes of Health (AI121985). LA - English DB - MTMT ER - TY - JOUR AU - Wood, Will AU - Martin, Paul TI - Macrophage Functions in Tissue Patterning and Disease: New Insights from the Fly JF - DEVELOPMENTAL CELL J2 - DEV CELL VL - 40 PY - 2017 IS - 3 SP - 221 EP - 233 PG - 13 SN - 1534-5807 DO - 10.1016/j.devcel.2017.01.001 UR - https://m2.mtmt.hu/api/publication/26698809 ID - 26698809 N1 - Department of Cellular and Molecular Medicine, Biomedical Sciences, University of Bristol, Bristol, BS8 1TD, United Kingdom Departments of Biochemistry and Physiology, Pharmacology and Neuroscience, Biomedical Sciences, University of Bristol, Bristol, BS8 1TD, United Kingdom School of Medicine, Cardiff University, Cardiff, CF14 4XN, United Kingdom Lee Kong Chiang School of Medicine, Nanyang Technological University, Singapore, 636921, Singapore Cited By :44 Export Date: 19 January 2022 CODEN: DCEEB Correspondence Address: Wood, W.; Department of Cellular and Molecular Medicine, United Kingdom; email: w.wood@bristol.ac.uk LA - English DB - MTMT ER - TY - JOUR AU - Yang, Hairu AU - Hultmark, Dan TI - Drosophila muscles regulate the immune response against wasp infection via carbohydrate metabolism JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 7 PY - 2017 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-017-15940-2 UR - https://m2.mtmt.hu/api/publication/27260896 ID - 27260896 LA - English DB - MTMT ER -