@article{MTMT:34562583, title = {Natural Deep Eutectic Solvents for the Extraction of Spilanthol from Acmella oleracea (L.) R.K.Jansen}, url = {https://m2.mtmt.hu/api/publication/34562583}, author = {Alperth, Fabian and Feistritzer, Theresa and Huber, Melanie and Kunert, Olaf and Bucar, Franz}, doi = {10.3390/molecules29030612}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {29}, unique-id = {34562583}, issn = {1420-3049}, abstract = {With a growing focus on green chemistry, the extraction of natural products with natural deep eutectic solvents (NADES), which are eutectic mixtures of hydrogen bond donors and acceptors, has become an ever-expanding field of research. However, the use of NADES for the extraction of spilanthol from Acmella oleracea (L.) R.K.Jansen has not yet been investigated. Therefore, in this study, 20 choline chloride-based NADES, and for comparison, ethanol, were used as green extraction agents for spilanthol from Acmella oleracea flower heads. The effects of time, water addition, and temperature on NADES extractions were investigated and analysed by HPLC-DAD quantification. Additionally, UHPLC-DAD-ESI-MSn results for dichloromethane extracts, as well as the isolation of spilanthol and other main constituents as reference compounds, are reported. The best green extraction results were achieved by choline chloride (ChCl) with 1,2-propanediol (P, 1:2 molar ratio, +20% water) at 244.58 µg/mL, comparable to yields with ethanol (245.93 µg/mL). Methylurea (MeU, 1:2, +20% water) also showed promising results as a hydrogen bond donor in combination with choline chloride (208.12 µg/mL). In further experiments with NADES ChCl/P (1:2) and ChCl/MeU (1:2), extraction time had the least effect on spilanthol extraction with NADES, while yield decreased with water addition over 20% and increased with extraction temperature up to 80 °C. NADES are promising extraction agents for the extraction of spilanthol, and these findings could lead to applicable extracts for medicinal purposes, due to their non-toxic constituents.}, year = {2024}, eissn = {1420-3049}, orcid-numbers = {Alperth, Fabian/0000-0002-9716-1665; Kunert, Olaf/0000-0002-7091-5973; Bucar, Franz/0000-0002-7417-616X} } @article{MTMT:34747043, title = {Comprehensive review of the phytochemistry, pharmacology, pharmacokinetics, and toxicology of alkamides (2016–2022)}, url = {https://m2.mtmt.hu/api/publication/34747043}, author = {Cai, F. and Wang, C.}, doi = {10.1016/j.phytochem.2024.114006}, journal-iso = {PHYTOCHEMISTRY}, journal = {PHYTOCHEMISTRY}, volume = {220}, unique-id = {34747043}, issn = {0031-9422}, abstract = {Alkamides refer to a class of natural active small-molecule products composed of fatty acids and amine groups. These compounds are widely distributed in plants, and their unique structures and various pharmacological activities have caught the attention of scholars. This review provides a collection of literatures related to the phytochemistry, pharmacological effects, pharmacokinetics, and toxicity of alkamides published in 2016–2022 and their summary to provide references for further development of this class of ingredients. A total of 234 components (including chiral isomers) were summarized, pharmacological activities, such as anti-inflammatory, antitumor, antidiabetic, analgesic, neuroprotective, insecticidal, antioxidant, and antibacterial, and miscellaneous properties of alkamides were discussed. In addition, the pharmacokinetic characteristics and toxicity of alkamides were reviewed. However, information on the pharmacological mechanisms of the action, drug safety, and pharmacokinetics of alkamides is limited and thus requires further investigation and evaluation. © 2024 Elsevier Ltd}, keywords = {PHARMACOKINETICS; PHARMACOLOGY; TOXICOLOGY; phytochemistry; alkamides}, year = {2024}, eissn = {1873-3700} } @article{MTMT:34562592, title = {Comprehensive review of the phytochemistry, pharmacology, pharmacokinetics, and toxicology of alkamides (2016–2022)}, url = {https://m2.mtmt.hu/api/publication/34562592}, author = {Cai, Fujie and Wang, Changhong}, doi = {10.1016/j.phytochem.2024.114006}, journal-iso = {PHYTOCHEMISTRY}, journal = {PHYTOCHEMISTRY}, unique-id = {34562592}, issn = {0031-9422}, year = {2024}, eissn = {1873-3700} } @article{MTMT:34747041, title = {DEFB114 protein enhances host resistance to fungal infection through the NOD1/2-ATG16L1-NF-κB signaling pathway}, url = {https://m2.mtmt.hu/api/publication/34747041}, author = {Chen, J. and Mei, Q. and Wang, L. and Wei, Y.}, doi = {10.1016/j.bioorg.2024.107245}, journal-iso = {BIOORG CHEM}, journal = {BIOORGANIC CHEMISTRY}, volume = {146}, unique-id = {34747041}, issn = {0045-2068}, abstract = {The overuse of antibiotics has led to the enhanced resistance of many pathogenic bacteria, posing a threat to human health. Therefore, there is a need to develop green and safe alternatives to antibiotics. Beta-defensins play a crucial role in host defense against pathogens and have multifunctional properties, exerting key roles in innate and adaptive immunity, as well as non-immune processes. In this study, a 210 bp long cDNA sequence of yak DEFB114 gene was amplified and successfully expressed in a prokaryotic system. The DEFB114 protein exhibited significant inhibitory effects on the growth of Aspergillus fumigatus in vitro. When co-cultured with yak macrophages, DEFB114 protein enhanced macrophage phagocytic activity and increased nucleic acid fluorescence intensity (P < 0.05). DEFB114 protein also enhanced the activity of yak macrophages stimulated by inactivated Aspergillus fumigatus spores, increased the release of nitric oxide (NO), and promoted the expression of genes such as γ-actin, Lgals, Man2b, and Capg (P < 0.05). In mice experiments, DEFB114 protein promoted resistance against Aspergillus fumigatus infection, by regulating the NOD1/2-ATG16L1-NF-κB pathway to modulate the host immune response and exert its anti-infective effects. In summary, the yak DEFB114 protein could inhibit the growth of Aspergillus fumigatus and enhance the animal's resistance to pathogenic microorganisms, thereby having significant implications in the treatment and prevention of fungal infections. © 2024 Elsevier Inc.}, keywords = {MACROPHAGES; Aspergillus fumigatus; yak; DEFB114}, year = {2024}, eissn = {1090-2120} } @article{MTMT:33858076, title = {Clinical Safety of a Pharmaceutical Formulation Containing an Extract of Acmella oleracea (L.) in Patients With Premature Ejaculation: A Pilot Study}, url = {https://m2.mtmt.hu/api/publication/33858076}, author = {Batista, Lecildo Lira and Koga, Rosemary de Carvalho Rocha and Teixeira, Abrahao Victor Tavares de Lima and Teixeira, Thiago Afonso and de Melo, Ester Lopes and Carvalho, Jose Carlos Tavares}, doi = {10.1177/15579883231167819}, journal-iso = {AM J MENS HEALTH}, journal = {AMERICAN JOURNAL OF MENS HEALTH}, volume = {17}, unique-id = {33858076}, issn = {1557-9883}, abstract = {Acmella oleracea (L.) R. K. Jansen (Asteraceae) is a plant species widely used in traditional Amazonian medicine to treat sexual dysfunction. The use of this plant has gained popularity because of its sensory properties, such as a tingling sensation. In this study on patients with premature ejaculation, we evaluated the clinical action of a nano-formulation containing an ethanolic extract of A. oleracea inflorescences. Major constituents in the extracts were identified based on gas chromatographic analysis. Participants used a spray preparation based on the A. oleracea extract for 12 weeks, during which they were instructed to apply the product 5 min prior to sexual intercourse. To assess therapeutic efficacy, participants were required to record the mean intravaginal latency time for ejaculation (IELT). During the period of spray treatment, the nano-formulation of A. oleracea increased participant IELT values (M = 293 s) compared with the baseline values (193 s). This nano-formulation reported clinical action in patients with premature ejaculation. It is accordingly considered to have potential application as a therapeutic alternative with benefits for both patients and their partners. Given the small number of participants in this study, further multicenter studies involving a larger number of participants are needed to confirm these observations.}, keywords = {sexual activity; sexual dysfunction; THERAPEUTIC EFFICACY; spilanthol; N-alkylamide}, year = {2023}, eissn = {1557-9891} } @article{MTMT:34088591, title = {Guiqi Baizhu prescription ameliorates cytarabine-induced intestinal mucositis by targeting JAK2 to inhibit M1 macrophage polarization}, url = {https://m2.mtmt.hu/api/publication/34088591}, author = {Chu, Wei and Li, Ya-ling and Li, Jun-jie and Lin, Jia and Li, Mi and Wang, Jiao and He, Jian-zheng and Zhang, Yue-mei and Yao, Juan and Jin, Xiao-jie and Cai, Hui and Liu, Yong-qi}, doi = {10.1016/j.biopha.2023.114902}, journal-iso = {BIOMED PHARMACOTHER}, journal = {BIOMEDICINE & PHARMACOTHERAPY}, volume = {164}, unique-id = {34088591}, issn = {0753-3322}, abstract = {Background: Intestinal mucositis (IM) is characterized by damage to the intestinal mucosa resulting from inhi-bition of epithelial cell division and loss of renewal capacity following anticancer chemotherapy and radio-therapy. Cytarabine (Ara-C), the main chemotherapy drug for the treatment of leukemia and lymphoma, is a frequent cause of IM. Guiqi Baizhu prescription (GQBZP) is a traditional Chinese medicine with anti-cancer and anti-inflammatory effects.Purpose: To determine if GQBZP can ameliorate Ara-C induced IM and identify and characterize the pharma-cologic and pharmacodynamic mechanisms.Study design and methods: IM was induced in mice with Ara-C and concurrently treated with orally administered GQBZP. Body weight and food intake was monitored, with HE staining to calculate ileal histomorphometric scoring and villus length/crypt depth. Immunoblotting was used to detect intestinal tissue inflammatory factors. M1 macrophages (M1) were labeled with CD86 by flow cytometry and iNOS + F4/80 by immunofluorescence. Virtual screening was used to find potentially active compounds in GQBZP that targeted JAK2. In vitro, RAW264.7 cells were skewed to M1 macrophage polarization by lipopolysaccharide (LPS) and interferon-gamma (INF-gamma) and treated orally with GQBZP or potential active compounds. M1 was labeled with CD86 by flow cytometry and iNOS by immunofluorescence. ELISA was used to detect inflammatory factor expression. Active compounds against JAK2, p-JAK2, STAT1 and p-STAT1 were identified by western blotting and HCS fluorescence. Molecular dynamics simulations and pharmacokinetic predictions were carried out on representative active compounds.Results: Experimental results with mice in vivo suggest that GQBZP significantly attenuated Ara-C-induced ileal damage and release of pro-inflammatory factors by inhibiting macrophage polarization to M1. Molecular docking was used to identify potentially active compounds in GQBZP that targeted JAK2, a key factor in macrophage polarization to M1. By examining the main components of each herb and applying Lipinski's rules, ten poten-tially active compounds were identified. In vitro experimental results suggested that all 10 compounds of GQBZP targeted JAK2 and could inhibit M1 polarization in RAW264.7 cells treated with LPS and INF-gamma. Among them, acridine and senkyunolide A down-regulated the expression of JAK2 and STAT1. MD simulations revealed that acridine and senkyunolide A were stable in the active site of JAK2 and exhibited good interactions with the surrounding amino acids.Conclusions: GQBZP can ameliorate Ara-C-induced IM by reducing macrophage polarization to M1, and acridine and senkyunolide A are representative active compounds in GQBZP that target JAK2 to inhibit M1 polarization. Targeting JAK2 to regulate M1 polarization may be a valuable therapeutic strategy for IM.}, keywords = {acridine; Macrophage polarization; JAK2; STAT1; senkyunolide A; Intestinal mucositis}, year = {2023}, eissn = {1950-6007} } @article{MTMT:34329631, title = {Acmella oleracea extracts as green pesticides against eight arthropods attacking stored products}, url = {https://m2.mtmt.hu/api/publication/34329631}, author = {Kavallieratos, Nickolas G. and Spinozzi, Eleonora and Filintas, Constantin S. and Nika, Erifili P. and Skourti, Anna and Panariti, Anna Maria E. and Ferrati, Marta and Petrelli, Riccardo and Ricciutelli, Massimo and Angeloni, Simone and Drenaggi, Ettore and Sensini, Alessia and Maggi, Filippo and Canale, Angelo and Benelli, Giovanni}, doi = {10.1007/s11356-023-28577-8}, journal-iso = {ENVIRON SCI POLLUT R}, journal = {ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH}, volume = {30}, unique-id = {34329631}, issn = {0944-1344}, abstract = {Developing sustainable control tools for managing noxious pests attacking stored foodstuffs is a timely research challenge. Acmella oleracea (L.) R. K. Jansen is a crop widely cultivated for its multiple usages on an industrial level. In this study, the extracts prepared with A. oleracea aerial parts were applied on wheat kernels for the management of eight important arthropod pests attacking stored products, i.e., Cryptolestes ferrugineus, Tenebrio molitor, Oryzaephilus surinamensis, Trogoderma granarium, Tribolium castaneum, Tribolium confusum, Alphitobius diaperinus (adults/larvae), and Acarus siro (adults/nymphs). Extraction of A. oleracea was optimized on the base of the yield and content of spilanthol and other N-alkylamides which were analysed by HPLC-DAD-MS. Two concentrations of n-hexane or methanol extracts (500 ppm and 1000 ppm), obtained through Soxhlet extraction, were tested to acquire mortality data on the above-mentioned pests after 4, 8, and 16 h and 1 to 7 days of exposure. Both extracts achieved complete mortality (100.0%) of C. ferrugineus adults. In the case of A. diaperinus adults, mortalities were very low at any concentrations of both extracts. In general, the n-hexane extract was more efficient than methanol extract against almost all species and stages. Considering both extracts, the susceptibility order, from most to least susceptible species/stage, was C. ferrugineus adults > A. diaperinus larvae > C. ferrugineus larvae > T. granarium adults > T. molitor larvae > O. surinamensis adults > O. surinamensis larvae > T. confusum larvae > T. castaneum larvae > A. siro adults > T. molitor adults > A. siro nymphs > T. granarium larvae > T. castaneum adults > T. confusum adults > A. diaperinus adults. Our research provides useful knowledge on the efficacy of N-alkylamides-rich A. oleracea extracts as grain protectants, pointing out the importance of targeting the most susceptible species/ developmental stages.}, keywords = {Adult; larva; nymph; Grain protectant; Plant-based pesticide; Stored-product pests}, year = {2023}, eissn = {1614-7499}, pages = {94904-94927}, orcid-numbers = {Kavallieratos, Nickolas G./0000-0001-5851-5013; Angeloni, Simone/0000-0001-6571-2242} } @article{MTMT:32690427, title = {Phytochemical Analysis, Antioxidant and Bone Anabolic Effects of Blainvillea acmella (L.) Philipson}, url = {https://m2.mtmt.hu/api/publication/32690427}, author = {Abdul, Rahim R. and Jayusman, P.A. and Lim, V. and Ahmad, N.H. and Abdul, Hamid Z.A. and Mohamed, S. and Muhammad, N. and Ahmad, F. and Mokhtar, N. and Mohamed, N. and Shuid, A.N. and Naina, Mohamed I.}, doi = {10.3389/fphar.2021.796509}, journal-iso = {FRONT PHARMACOL}, journal = {FRONTIERS IN PHARMACOLOGY}, volume = {12}, unique-id = {32690427}, year = {2022}, eissn = {1663-9812} } @article{MTMT:33153168, title = {OGG1 Inhibition Reduces Acinar Cell Injury in a Mouse Model of Acute Pancreatitis}, url = {https://m2.mtmt.hu/api/publication/33153168}, author = {Hajnády, Zoltán and Nagy-Pénzes, Máté and Demény, Máté Ágoston and Kovács, Katalin and El-Hamoly, Tarek and Maléth, József and Hegyi, Péter and Polgár, Zsuzsanna and Hegedűs, Csaba and Virág, László}, doi = {10.3390/biomedicines10102543}, journal-iso = {BIOMEDICINES}, journal = {BIOMEDICINES}, volume = {10}, unique-id = {33153168}, abstract = {Acute pancreatitis (AP) is a potentially life-threatening gastrointestinal disease with a complex pathology including oxidative stress. Oxidative stress triggers oxidative DNA lesions such as formation of 7,8-dihydro-8-oxo-2′-oxoguanine (8-oxoG) and also causes DNA strand breaks. DNA breaks can activate the nuclear enzyme poly(ADP-ribose) polymerase 1 (PARP1) which contributes to AP pathology. 8-oxoG is recognized by 8-oxoG glycosylase 1 (OGG1) resulting in the removal of 8-oxoG from DNA as an initial step of base excision repair. Since OGG1 also possesses a DNA nicking activity, OGG1 activation may also trigger PARP1 activation. In the present study we investigated the role played by OGG1 in AP. We found that the OGG1 inhibitor compound TH5487 reduced edema formation, inflammatory cell migration and necrosis in a cerulein-induced AP model in mice. Moreover, TH5487 caused 8-oxoG accumulation and reduced tissue poly(ADP-ribose) levels. Consistent with the indirect PARP inhibitory effect, TH5487 shifted necrotic cell death (LDH release and Sytox green uptake) towards apoptosis (caspase activity) in isolated pancreatic acinar cells. In the in vivo AP model, TH5487 treatment suppressed the expression of various cytokine and chemokine mRNAs such as those of TNF, IL-1β, IL1ra, IL6, IL16, IL23, CSF, CCL2, CCL4, CCL12, IL10 and TREM as measured with a cytokine array and verified by RT-qPCR. As a potential mechanism underlying the transcriptional inhibitory effect of the OGG1 inhibitor we showed that while 8-oxoG accumulation in the DNA facilitates NF-κB binding to its consensus sequence, when OGG1 is inhibited, target site occupancy of NF-κB is impaired. In summary, OGG1 inhibition provides protection from tissue injury in AP and these effects are likely due to interference with the PARP1 and NF-κB activation pathways.}, year = {2022}, eissn = {2227-9059}, orcid-numbers = {Demény, Máté Ágoston/0000-0003-4623-4091; Maléth, József/0000-0001-5768-3090; Hegyi, Péter/0000-0003-0399-7259} } @article{MTMT:32785087, title = {Promotion effects of steam-dried Betula platyphylla extract on hair regrowth}, url = {https://m2.mtmt.hu/api/publication/32785087}, author = {Jeong, Won Ahn and Su, Kil Jang and Bo, Ram Jo and Hyun, Soo Kim and Eui, Young Jeoung and Kithenya, Hillary and Yeong, Min Yoo and Seong, Soo Joo}, doi = {10.9721/KJFST.2022.54.1.43}, journal-iso = {KOREAN J FOOD SCI TECHNOL}, journal = {KOREAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY}, volume = {54}, unique-id = {32785087}, issn = {0367-6293}, year = {2022}, pages = {43-51} } @article{MTMT:32886684, title = {Tricetin Reduces Inflammation and Acinar Cell Injury in Cerulein-Induced Acute Pancreatitis: The Role of Oxidative Stress-Induced DNA Damage Signaling}, url = {https://m2.mtmt.hu/api/publication/32886684}, author = {Nagy-Pénzes, Máté and Hajnády, Zoltán and Regdon, Zsolt and Demény, Máté Ágoston and Kovács, Katalin and El-Hamoly, Tarek and Maléth, József and Hegyi, Péter and Hegedűs, Csaba and Virág, László}, doi = {10.3390/biomedicines10061371}, journal-iso = {BIOMEDICINES}, journal = {BIOMEDICINES}, volume = {10}, unique-id = {32886684}, year = {2022}, eissn = {2227-9059}, orcid-numbers = {Maléth, József/0000-0001-5768-3090; Hegyi, Péter/0000-0003-0399-7259} } @article{MTMT:33344133, title = {A Review of the Chemistry and Biological Activities of Acmella oleracea ("jambu", Asteraceae), with a View to the Development of Bioinsecticides and Acaricides}, url = {https://m2.mtmt.hu/api/publication/33344133}, author = {Spinozzi, Eleonora and Ferrati, Marta and Baldassarri, Cecilia and Cappellacci, Loredana and Marmugi, Margherita and Caselli, Alice and Benelli, Giovanni and Maggi, Filippo and Petrelli, Riccardo}, doi = {10.3390/plants11202721}, journal-iso = {PLANTS-BASEL}, journal = {PLANTS-BASEL}, volume = {11}, unique-id = {33344133}, abstract = {Human pathologies, environmental pollution, and resistance phenomena caused by the intensive use of chemical pesticides have shifted the attention of the agrochemical industries towards eco-friendly insecticides and acaricides. Acmella oleracea (L.) R. K. Jansen (jambu) is a plant native to South America, widely distributed and cultivated in many countries due to its numerous pharmacological properties. This review analyzes literature about the plant, its uses, and current knowledge regarding insecticidal and acaricidal activity. Acmella oleracea has proven to be a potential pesticide candidate against several key arthropod pest and vector species. This property is inherent to its essential oil and plant extract, which contain spilanthol, the main representative of N-alkylamides. As a result, there is a scientific basis for the industrial exploitation of jambu in the preparation of green insecticides. However, studies related to its toxicity towards non-target species and those aimed at formulating and developing marketable products are lacking.}, keywords = {IN-VITRO; MOTH; EXTRACT; ticks; micropropagation; mosquito; biopesticide; Larvicidal activity; acaricidal activity; spilanthol; Stored-product pest; eco-friendly insecticide; R. K. JANSEN; L. RK JANSEN; SPILANTHES-ACMELLA; TOOTHACHE PLANT; ISOBUTYLAMIDES}, year = {2022}, eissn = {2223-7747}, orcid-numbers = {Spinozzi, Eleonora/0000-0002-1216-1368; Maggi, Filippo/0000-0003-1375-4744; Petrelli, Riccardo/0000-0002-4760-1204} } @article{MTMT:32014709, title = {Potential Antioxidant and Anti-Inflammatory Effects of Spilanthes acmella and Its Health Beneficial Effects: A Review}, url = {https://m2.mtmt.hu/api/publication/32014709}, author = {Abdul, Rahim Rohanizah and Jayusman, Putri Ayu and Muhammad, Norliza and Mohamed, Norazlina and Lim, Vuanghao and Ahmad, Nor Hazwani and Mohamad, Sharlina and Abdul, Hamid Zuratul Ain and Ahmad, Fairus and Mokhtar, Norfilza and Shuid, Ahmad Nazrun and Mohamed, Isa Naina}, doi = {10.3390/ijerph18073532}, journal-iso = {INT J ENV RES PUB HE}, journal = {INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH}, volume = {18}, unique-id = {32014709}, issn = {1661-7827}, abstract = {Oxidative stress and inflammation are two common risk factors of various life-threatening disease pathogenesis. In recent years, medicinal plants that possess antioxidant and anti-inflammatory activities were extensively studied for their potential role in treating and preventing diseases. Spilanthes acmella (S. acmella), which has been traditionally used to treat toothache in Malaysia, contains various active metabolites responsible for its anti-inflammatory, antiseptic, and anesthetic bioactivities. These bioactivities were attributed to bioactive compounds, such as phenolic, flavonoids, and alkamides. The review focused on the summarization of in vitro and in vivo experimental reports on the antioxidant and anti-inflammatory actions of S. acmella, as well as how they contributed to potential health benefits in lowering the risk of diseases that were related to oxidative stress. The molecular mechanism of S. acmella in reducing oxidative stress and inflammatory targets, such as inducible nitric oxide synthase (iNOS), transcription factors of the nuclear factor-kappa B family (NF-kappa B), cyclooxygenase-2 (COX-2), and mitogen-activated protein kinase (MAPK) signaling pathways were discussed. Besides, the antioxidant potential of S. acmella was measured by total phenolic content (TPC), total flavonid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and superoxide anion radical scavenging (SOD) and thiobarbituric acid reactive substance (TBARS) assays. This review revealed that S. acmella might have a potential role as a reservoir of bioactive agents contributing to the observed antioxidant, anti-inflammatory, and health beneficial effects.}, keywords = {ANTIOXIDANT; COX-2; anti-inflammatory; B; Environmental Sciences; INOS; Spilanthes acmella; NF-κ}, year = {2021}, eissn = {1660-4601}, orcid-numbers = {Abdul, Hamid Zuratul Ain/0000-0002-5267-6091} } @article{MTMT:32778812, title = {Comparative Evaluation of Efficacy of Spilanthes Acmella Flower Extract as an Intracanal Medicament with Calcium hydroxide and 2%Chlorhexidine gel –An in Vitro Study}, url = {https://m2.mtmt.hu/api/publication/32778812}, author = {Anamika, Sinha and Ashish, Jain and Sheetal, Mali and Amit, Patil and Himmat, Jaiswal and Hrishikesh, Saoji}, journal-iso = {ANNALS ROMANIAN SOC CELL BIOL}, journal = {ANNALS OF THE ROMANIAN SOCIETY FOR CELL BIOLOGY}, volume = {25}, unique-id = {32778812}, issn = {1583-6258}, year = {2021}, eissn = {2067-8282}, pages = {6773-6782} } @article{MTMT:31938546, title = {Poly(ADP-ribose) polymerase 1 promotes inflammation and fibrosis in a mouse model of chronic pancreatitis}, url = {https://m2.mtmt.hu/api/publication/31938546}, author = {El-Hamoly, T and Hajnády, Zoltán and Nagy-Pénzes, Máté and Bakondi, Edina and Regdon, Zsolt and Demény, Máté Ágoston and Kovács, Katalin and Hegedűs, Csaba and Abd, El-Rahman SS and Szabó, Éva and Maléth, József and Hegyi, Péter and Virág, László}, doi = {10.3390/ijms22073593}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {22}, unique-id = {31938546}, issn = {1661-6596}, year = {2021}, eissn = {1422-0067}, orcid-numbers = {Maléth, József/0000-0001-5768-3090; Hegyi, Péter/0000-0003-0399-7259} } @article{MTMT:32778813, title = {A Review of Medicinal Plants Traditionally used to Treat Male Sexual Dysfunctions – the Overlooked Potential of Acmella oleracea (L.) R.K. Jansen}, url = {https://m2.mtmt.hu/api/publication/32778813}, author = {Lira Batista, Lecildo and Corrêa do Nascimento, Lucas and Guimarães, Gabriel Francisconi and Matias Pereira, Arlindo César and Rocha Koga, Rosemary de Carvalho and Teixeira dos Santos, Abrahão Victor Tavares de Lima and Fernandes, Caio Pinho and Teixeira, Thiago Afonso and Hu, Yutao and Hu, Xuebo and Carvalho, José Carlos Tavares}, doi = {10.5530/phrev.2021.15.1}, journal-iso = {PHARMACOGN REV}, journal = {PHARMACOGNOSY REVIEWS}, volume = {15}, unique-id = {32778813}, issn = {0973-7847}, year = {2021}, eissn = {0976-2787}, pages = {01-11} } @article{MTMT:32160359, title = {Exploiting the Reversibility of GT(BP1) Catalyzed One-pot Reactions for the Synergistical Synthesis of Ponasterone A and Phenolic Glycosides}, url = {https://m2.mtmt.hu/api/publication/32160359}, author = {Li, Yuqiang and Li, Bingfeng and Chen, Tianyi and Wu, Bin and Chu, Jianlin and He, Bingfang}, doi = {10.1007/s12257-020-0135-1}, journal-iso = {BIOTECHNOL BIOPROC E}, journal = {BIOTECHNOLOGY AND BIOPROCESS ENGINEERING}, volume = {26}, unique-id = {32160359}, issn = {1226-8372}, year = {2021}, eissn = {1976-3816}, pages = {408-418}, orcid-numbers = {Chu, Jianlin/0000-0001-8197-6459} } @article{MTMT:32116836, title = {Tulipiferamide A, an Alkamide from Liriodendron tulipifera, Exhibits an Anti-Inflammatory Effect via Targeting IKKβ Phosphorylation}, url = {https://m2.mtmt.hu/api/publication/32116836}, author = {Park, I. and Byun, H.S. and Hur, G.M. and Na, M.}, doi = {10.1021/acs.jnatprod.1c00146}, journal-iso = {J NAT PROD}, journal = {JOURNAL OF NATURAL PRODUCTS}, volume = {84}, unique-id = {32116836}, issn = {0163-3864}, abstract = {Three new alkamides, tulipiferamides A-C (1-3, respectively), and 30 known compounds (4-33) were obtained from the roots of Liriodendron tulipifera (Magnoliaceae). Dehydrotemisin (4), an elemane sesquiterpene lactone, was isolated for the first time from nature. The structures were deduced by the interpretation of NMR spectroscopic and MS spectral data. The geometries of the double bonds in tulipiferamides A-C (1-3, respectively) were determined on the basis of 1H-1H coupling constants and 13C chemical shifts. The presence of the alkamide type in this plant is reported for the first time. An analysis of the inflammatory response revealed that seven compounds (1, 4, 7, 9, 14, 23, and 27) suppressed the nitric oxide production induced by LPS in RAW264.7 macrophages. Furthermore, tulipiferamide A (1) inhibits NF-κB activation by selectively targeting IKKβ, an upstream kinase of NF-κB, resulting in the suppression of inflammatory mediators, including iNOS, COX-2, IL-1β, TNFα, and IL-6. Our results provide a rationale for the further development of tulipiferamide A as a selective IKKβ inhibitor to modulate inflammatory diseases. © 2021 American Chemical Society and American Society of Pharmacognosy.}, year = {2021}, eissn = {1520-6025}, pages = {1598-1606} } @article{MTMT:31831963, title = {High‐content screening identifies inhibitors of oxidative stress‐induced parthanatos: cytoprotective and anti‐inflammatory effects of ciclopirox}, url = {https://m2.mtmt.hu/api/publication/31831963}, author = {Regdon, Zsolt and Demény, Máté Ágoston and Kovács, Katalin and Hajnády, Zoltán and Nagy-Pénzes, Máté and Bakondi, Edina and Kiss, Alexandra and Hegedűs, Csaba and Virág, László}, doi = {10.1111/bph.15344}, journal-iso = {BR J PHARMACOL}, journal = {BRITISH JOURNAL OF PHARMACOLOGY}, volume = {178}, unique-id = {31831963}, issn = {0007-1188}, abstract = {Background and Purpose Excessive oxidative stress can induce PARP1‐mediated programmed necrotic cell death, termed parthanatos. Inhibition of parthanatos may be therapeutically beneficial in a wide array of diseases associated with tissue injury and inflammation. Our goal was to identify novel molecules inhibiting parthanatos. Experimental Approach A small library of 774 pharmacologically active compounds was screened in a Sytox Green uptake assay, which identified 20 hits that reduced hydrogen‐peroxide‐induced parthanatos with an efficiency comparable to the benchmark PARP inhibitor, PJ34. Key Results Of these hits, two compounds, antifungal ciclopirox and dopamine receptor agonist apomorphine, inhibited PAR polymer synthesis. These two compounds prevented the binding of PARP1 to oxidatively damaged DNA but did not directly interfere with the interaction between DNA and PARP1. Both compounds inhibited mitochondrial superoxide and H2O2 production and suppressed DNA breakage. Since H2O2‐induced damage is dependent on Fe2+‐catalysed hydroxyl radical production (Fenton chemistry), we determined the iron chelation activity of the two test compounds and found that ciclopirox and, to a lesser extent, apomorphine act as iron chelators. We also show that the Fe2+ chelation and indirect PARP inhibitory effects of ciclopirox translate to anti‐inflammatory actions as demonstrated in a mouse dermatitis model, where ciclopirox reduced ear swelling, inflammatory cell recruitment and poly(ADP‐ribosyl)ation. Conclusion and Implications Our findings indicate that the antimycotic drug, ciclopirox, acts as an iron chelator and thus targets an early event in hydrogen‐peroxide‐induced parthanatos. Ciclopirox has the potential to be repurposed as a cytoprotective and anti‐inflammatory agent.}, keywords = {Inflammation; CYTOPROTECTION; Cell Death; PARP1; Regulated necrosis; Oxidative stress}, year = {2021}, eissn = {1476-5381}, pages = {1095-1113} } @article{MTMT:32498022, title = {Spilanthol-rich essential oil obtained by microwave-assisted extraction from Acmella oleracea (L.) R.K. Jansen and its nanoemulsion: Insecticidal, cytotoxic and anti-inflammatory activities}, url = {https://m2.mtmt.hu/api/publication/32498022}, author = {Spinozzi, E. and Pavela, R. and Bonacucina, G. and Perinelli, D.R. and Cespi, M. and Petrelli, R. and Cappellacci, L. and Fiorini, D. and Scortichini, S. and Garzoli, S. and Angeloni, C. and Freschi, M. and Hrelia, S. and Quassinti, L. and Bramucci, M. and Lupidi, G. and Sut, S. and Dall'Acqua, S. and Benelli, G. and Canale, A. and Drenaggi, E. and Maggi, F.}, doi = {10.1016/j.indcrop.2021.114027}, journal-iso = {IND CROP PROD}, journal = {INDUSTRIAL CROPS AND PRODUCTS}, volume = {172}, unique-id = {32498022}, issn = {0926-6690}, abstract = {Acmella oleracea (L.) R.K. Jansen, also named jambù, is an edible and medicinal plant native to Brazil but extensively cultivated all over the world due to its diverse utilizations in food, cosmetics, pharmaceutics, and pest management science. Most of applications are related to the presence of active ingredients, namely alkylamides, among which spilanthol is the most important one. On the other hand, the plant biomass resulting from the large-scale cultivation of A. oleracea may yield an essential oil (EO), which can be used for some industrial purposes, for instance as larvicide against vector mosquitoes. This EO is mainly characterized by mono- and sesquiterpenes, however also spilanthol can be detected in the mixture. To maximize the content of spilanthol in the A. oleracea EO, A. oleracea aerial parts obtained from a cultivation in central Italy were subjected to microwave-assisted extraction (MAE). This procedure allowed to obtain a higher yield and spilanthol content when compared with traditional hydrodistillation (0.47 v 0.22 %, and 13.31 vs 2.24 %, respectively). The EO terpene fraction was mainly represented by β-pinene (10.8 %), myrcene (12.3 %), (E)-caryophyllene (19.4 %) and α-humulene (1.1 %). The A. oleracea EO was encapsulated into a nanoemulsion (NE) containing 6 % EO using a high-energy method. The formulation physical stability was assessed by optical microscope and DLS analyses at different timepoints showing stability up to one year of storage. The A. oleracea EO and its NE, along with the isolated spilanthol, were evaluated for acute toxicity against the 3rd instar larvae of Culex quinquefasciatus, a filariasis and arbovirus vector of public health importance, achieving LC50 values of 16.1, 407.5, and 3.1 μL/L, respectively. The larvicidal activity seemed to be not dependent on the interaction with the cholinergic system, as shown by the high IC50 values of A. oleracea EO and spilanthol exhibited in the anti-acetylcholinesterase (AChE) assay. Testing LC30 values, the A. oleracea-borne products showed a significant impact on larval development, fecundity, fertility and natality of C. quinquefasciatus. Testing LC30 values, the A. oleracea EO-NE provided a higher efficacy than spilanthol, reducing the adult fertility, in terms of egg hatchability (%) and the overall abundance of F1 larvae. These results can be considered as promising for the development of new mosquito larvicides. The safety use of A. oleracea EO, NE and spilanthol was demonstrated in assays on mammalian fibroblasts and microglia cells, showing low level of cytotoxicity coupled with protective effects against inflammation. © 2021 Elsevier B.V.}, keywords = {EXTRACTION; insecticide; BRAZIL; Italy; Mammalia; OLEFINS; VIRUSES; ANTIMICROBIAL ACTIVITY; ESSENTIAL OILS; Monoterpenes; herb; Essential oil; emulsion; Nanotechnology; anti-inflammatory activity; extraction method; Microwave-assisted extraction; Antennas; CULICIDAE; CULICIDAE; CULICIDAE; Cytotoxic; Arbovirus; Culex quinquefasciatus; Culex quinquefasciatus; nanoemulsion; Edible plants; Mosquito control; Mosquito control; larvicide; larvicide; Culex pipiens quinquefasciatus; Essential oil nanoemulsion; Essential oil nanoemulsion; Jambù; Jambù; Acmella}, year = {2021}, eissn = {1872-633X} } @article{MTMT:32014708, title = {Chymase inhibition: A key factor in the anti-inflammatory activity of ethanolic extracts and spilanthol isolated from Acmella oleracea}, url = {https://m2.mtmt.hu/api/publication/32014708}, author = {Stein, Renan and Berger, Markus and de, Cecco Bianca Santana and Mallmann, Luana Peixoto and Terraciano, Paula Barros and Driemeier, David and Rodrigues, Eliseu and Beys-da-Silva, Walter Orlando and Konrath, Eduardo Luis}, doi = {10.1016/j.jep.2020.113610}, journal-iso = {J ETHNOPHARMACOL}, journal = {JOURNAL OF ETHNOPHARMACOLOGY}, volume = {270}, unique-id = {32014708}, issn = {0378-8741}, abstract = {Ethnopharmacological relevance: Acmella oleracea (L.) R. K. Jansen (Asteraceae), known as jambii in Brazil, is used in traditional medicine as analgesic and for inflammatory conditions, characterized by the presence of N-alkylamides, mainly spilanthol. This bioactive compound is responsible for the above-described pharmacological properties, including sialagogue and anesthetic. Aim of the study: This study aimed to characterize the anti-inflammatory effects of A. oleracea leaves (AOEE-L) and flowers (AOEE-F) extracts, including an isolated alkylamide (spilanthol), using in vitro and in vivo models. The mechanism underlying this effect was also investigated. Materials and methods: Extracts were analyzed by HPLC-ESI-MS/MS in order to characterize the N-alkylamides content. AOEE-L, AOEE-F (25-100 mu g/mL) and spilanthol (50-200 mu M) were tested in vitro on VSMC after stimulation with hyperglycemic medium (25 mM glucose). Their effects over nitric oxide (NO) generation, chymase inhibition and expression, catalase (CAT), superoxide anion (SOD) radical activity were evaluated. After an acute administration of extracts (10-100 mg/mL) and spilanthol (6.2 mg/mL), the anti-inflammatory effects were evaluated by applying the formalin test in rats. Blood was collected to measure serum aminotransferases activities, NO activity, creatinine and urea. Results: A number of distinct N-alkylamides were detected and quantified in AOEE-L and AOEE-F. Spilanthol was identified in both extracts and selected for experimental tests. Hyperglycemic stimulation in VSMC promoted the expression of inflammatory parameters, including chymase, NO, CAT and SOD activity and chymase expression, all of them attenuated by the presence of the extracts and spilanthol. The administration of extracts or spilanthol significantly inhibited edema formation, NO production and cell tissue infiltration in the formalin test, without causing kidney and liver toxicity. Conclusion: Taken together, these results provide evidence for the anti-inflammatory activity of leaves and flowers extracts of jambii associated distinctly with their chemical profile. The effects appear to be associated with the inhibition of chymase activity, suppression of the proinflammatory cytokine NO and antioxidant activities.}, keywords = {Inflammation; Chemistry, Medicinal; Plant Sciences; Integrative & Complementary Medicine; spilanthol; Acmella oleracea; N-alkylamides}, year = {2021}, eissn = {1872-7573}, orcid-numbers = {Rodrigues, Eliseu/0000-0003-4199-469X} } @article{MTMT:31492246, title = {Caseahomopene A, a ring-expanded homotriterpenoid from Casearia kurzii showing anti-inflammatory activities in vitro and in vivo}, url = {https://m2.mtmt.hu/api/publication/31492246}, author = {An, Lijun and Ma, Jun and Yang, Xueyuan and Liang, Yue and Wang, Huimei and Tuerhong, Muhetaer and Lall, Namrita and Abudukeremu, Munira and Zhang, Yi and Lee, Dongho and Xu, Jing and Wu, Xiaohui and Guo, Yuanqiang}, doi = {10.1016/j.bioorg.2020.103758}, journal-iso = {BIOORG CHEM}, journal = {BIOORGANIC CHEMISTRY}, volume = {98}, unique-id = {31492246}, issn = {0045-2068}, abstract = {Caseahomopene A (1), a rare natural product with a ring-expanded homotriterpenoid skeleton, was isolated from the leaves of Casearia kurzii. The structure including the absolute configuration was determined by spectroscopic data and X-ray crystallography analysis. Compound 1 showed anti-inflammatory effects in vitro and in vivo using LPS-stimulated cell and zebrafish model. As a potential anti-inflammatory agent, the anti-inflammatory mechanism of 1 was also investigated.}, keywords = {Anti-inflammation; Molecular docking; Zebrafish model; Homotriterpenoid; Casearia kurzii}, year = {2020}, eissn = {1090-2120}, orcid-numbers = {Lall, Namrita/0000-0002-3242-3476} } @article{MTMT:31726917, title = {Analytical and preparative chromatographic approaches for extraction of spilanthol from Acmella oleracea flowers}, url = {https://m2.mtmt.hu/api/publication/31726917}, author = {Balieiro, Odiane C. and Pinheiro, Mayra Suelen da Silva and Silva, Simone Y. S. and Oliveira, Marilene Nunes and Silva, Sebastiao C. and Gomes, Adriano A. and Pinto, Licarion}, doi = {10.1016/j.microc.2020.105035}, journal-iso = {MICROCHEM J}, journal = {MICROCHEMICAL JOURNAL}, volume = {157}, unique-id = {31726917}, issn = {0026-265X}, abstract = {Acmella oleracea, a plant with high spilanthol content, can be found in Brazilian north region. Despite the commercial interest of spilanthol for local communities and industries, due to its anesthetic properties, there are few works that have made a chemical study of it and almost none with analytical strategies. In this work, the influence of 4 solvents, namely acetonitrile, methanol, dichloromethane and ethanol, chosen from those mainly reported in published papers and their interaction in the extraction of spilanthol from Acmella oleracea flowers has been studied. The influence of each solvent proportions was discussed, and pure ethanol was selected as the one with better extraction efficiency. A model was built using the spilanthol peak area obtained by a HPLC-DAD system. Using the optimized solvent, it was possible to extract spilanthol from up to 10 g of grounded flowers using 25 mL of the solvent, producing an extract highly concentrated. The spilanthol content in this extract was isolated and characterized by liquid chromatography coupled to a triple quadrupole mass spectrometer. Therefore, this work presents an extensive analytical study of the spilanthol optimum extraction procedure, purification of the standard, validation of the analytical line and evaluation of the extraction efficiency with a recovery test.}, keywords = {liquid chromatography; experimental design; spilanthol; Acmella oleracea}, year = {2020}, eissn = {1095-9149}, orcid-numbers = {Pinto, Licarion/0000-0002-8682-6174} } @article{MTMT:32014711, title = {Determination of macronutrients, micronutrients and heavy metals present in spilanthes acmella hutch and dalz: Possible health effects}, url = {https://m2.mtmt.hu/api/publication/32014711}, author = {Bhopen, Singh S. and Singh, K. and Singh, Butola S. and Rawat, S. and Arunachalam, K.}, doi = {10.20307/nps.2020.26.1.50}, journal-iso = {NAT PROD SCI}, journal = {NATURAL PRODUCT SCIENCES}, volume = {26}, unique-id = {32014711}, issn = {1226-3907}, year = {2020}, pages = {50-58} } @article{MTMT:31035042, title = {Acmella oleracea for pain management}, url = {https://m2.mtmt.hu/api/publication/31035042}, author = {Rondanelli, M. and Fossari, F. and Vecchio, V. and Braschi, V. and Riva, A. and Allegrini, P. and Petrangolini, G. and Iannello, G. and Faliva, M.A. and Peroni, G. and Nichetti, M. and Gasparri, C. and Spadaccini, D. and Infantino, V. and Mustafa, S. and Alalwan, T. and Perna, S.}, doi = {10.1016/j.fitote.2019.104419}, journal-iso = {FITOTERAPIA}, journal = {FITOTERAPIA}, volume = {140}, unique-id = {31035042}, issn = {0367-326X}, abstract = {Despite advances in medicine and numerous agents that counteract pain, millions of patients continue to suffer. Attention has been given to identify novel botanical interventions that produce analgesia by interacting with nociceptive-transducing channels. The aim of this review is to provide an overview of the actual knowledge of Acmella oleracea (L.) and its activities, particularly those that are anti-inflammatory, anti-oxidant, and painkiller. These activities are attributed to numerous bioactive compounds, such as phytosterols, phenolic compounds and N-alkylamides (spilanthol, responsible for many activities, primarily anesthetic). This review includes 99 eligible studies to consider the anti-inflammatory, anti-oxidant, and painkiller of Acmella. Studies reported in this review confirmed anti-inflammatory and anti-oxidant activities of Acmella, postulating that transcription factors of the nuclear factor-κB family (NF-κB) trigger the transcription iNOS and COX-2 and several other pro-inflammatory mediators, such as IL-6, IL-1β, and TNF-α. The antinociceptive effects has been demonstrated and have been related to different processes, including inhibition of prostaglandin synthesis, activation of opioidergic, serotoninergic and GABAergic systems, and anesthetic activity through blockage of voltage-gated Na Channels. acmella oleracea represents a promise for pain management, particularly in chronic degenerative diseases, where pain is a significant critical issue. © 2019 Elsevier B.V.}, keywords = {PAIN; ANALGESIA; ANTIINFLAMMATORY ACTIVITY; ASTERACEAE; review; human; Cyclooxygenase 2; priority journal; nonhuman; interleukin 6; Tumor Necrosis Factor; antioxidant activity; AMIDE; immunoglobulin enhancer binding protein; herbaceous agent; interleukin 1beta; GABAergic system; drug activity; mouth disease; inducible nitric oxide synthase; phenol derivative; mouth lesion; plant leaf; phytosterol; PROSTAGLANDIN SYNTHESIS; Dietary supplement; spilanthol; Acmella oleracea; Acmella oleracea; Spilanthes acmella; anesthetic activity; throat disease}, year = {2020}, eissn = {1873-6971} } @article{MTMT:32778811, title = {Importance of nutrigenomics and nutrigenetics in food Science}, url = {https://m2.mtmt.hu/api/publication/32778811}, author = {TGG, Uthpala and HN, Fernando and Amila, Thibbotuwawa and M, Jayasinghe}, journal-iso = {MOJ FOOD PROC TECH}, journal = {MOJ FOOD PROCESSING AND TECHNOLOGY}, volume = {8}, unique-id = {32778811}, year = {2020}, eissn = {2381-182X}, pages = {114-119} } @article{MTMT:32014712, title = {Effects of seasonal variation on antioxidant potential and metabolite contents of Spilanthes acmella hutch and dalz}, url = {https://m2.mtmt.hu/api/publication/32014712}, author = {Singh, S.B. and Singh, K. and Butola, S.S. and Rawat, S. and Arunachalam, K.}, doi = {10.5958/0975-6892.2019.00049.2}, journal-iso = {MED PLANTS}, journal = {MEDICINAL PLANTS}, volume = {11}, unique-id = {32014712}, issn = {0975-4261}, year = {2019}, eissn = {0975-6892}, pages = {380-386} }