@article{MTMT:34446740, title = {Distinctive features of Zaprionus indianus hemocyte differentiation and function revealed by transcriptomic analysis}, url = {https://m2.mtmt.hu/api/publication/34446740}, author = {Cinege, Gyöngyi Ilona and Magyar, Lilla Brigitta and Kovács, Henrietta and Varga, Viktória and Bodai, László and Zsindely, Nóra and Nagy, Gábor and Hegedűs, Zoltán and Hultmark, Dan and Andó, István}, doi = {10.3389/fimmu.2023.1322381}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {14}, unique-id = {34446740}, issn = {1664-3224}, year = {2023}, eissn = {1664-3224}, orcid-numbers = {Bodai, László/0000-0001-8411-626X; Zsindely, Nóra/0000-0002-6189-3100; Nagy, Gábor/0000-0001-5464-1135; Andó, István/0000-0002-4648-9396} } @article{MTMT:33744872, title = {Evolution of insect innate immunity through domestication of bacterial toxins}, url = {https://m2.mtmt.hu/api/publication/33744872}, author = {Verster, Kirsten I. and Cinege, Gyöngyi Ilona and Lipinszki, Zoltán and Magyar, Lilla Brigitta and Kurucz, Judit Éva and Tarnopol, Rebecca L. and Ábrahám, Edit and Darula, Zsuzsanna and Karageorgi, Marianthi and Tamsil, Josephine A. and Akalu, Saron M. and Andó, István and Whiteman, Noah K.}, doi = {10.1073/pnas.2218334120}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {120}, unique-id = {33744872}, issn = {0027-8424}, abstract = {Toxin cargo genes are often horizontally transferred by phages between bacterial species and are known to play an important role in the evolution of bacterial pathogenesis. Here, we show how these same genes have been horizontally transferred from phage or bacteria to animals and have resulted in novel adaptations. We discovered that two widespread bacterial genes encoding toxins of animal cells, cytolethal distending toxin subunit B ( cdtB ) and apoptosis-inducing protein of 56 kDa ( aip56) , were captured by insect genomes through horizontal gene transfer from bacteria or phages. To study the function of these genes in insects, we focused on Drosophila ananassae as a model. In the D. ananassae subgroup species, cdtB and aip56 are present as singular ( cdtB ) or fused copies ( cdtB::aip56 ) on the second chromosome. We found that cdtB and aip56 genes and encoded proteins were expressed by immune cells, some proteins were localized to the wasp embryo’s serosa, and their expression increased following parasitoid wasp infection. Species of the ananassae subgroup are highly resistant to parasitoid wasps, and we observed that D. ananassae lines carrying null mutations in cdtB and aip56 toxin genes were more susceptible to parasitoids than the wild type. We conclude that toxin cargo genes were captured by these insects millions of years ago and integrated as novel modules into their innate immune system. These modules now represent components of a heretofore undescribed defense response and are important for resistance to parasitoid wasps. Phage or bacterially derived eukaryotic toxin genes serve as macromutations that can spur the instantaneous evolution of novelty in animals.}, year = {2023}, eissn = {1091-6490}, orcid-numbers = {Lipinszki, Zoltán/0000-0002-2067-0832; Tarnopol, Rebecca L./0000-0001-5675-8513; Karageorgi, Marianthi/0000-0003-2934-7019; Tamsil, Josephine A./0000-0002-3173-1848; Andó, István/0000-0002-4648-9396; Whiteman, Noah K./0000-0003-1448-4678} } @article{MTMT:32524824, title = {Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids}, url = {https://m2.mtmt.hu/api/publication/32524824}, author = {Cinege, Gyöngyi Ilona and Magyar, Lilla Brigitta and Kovács, Attila Lajos and Lerner, Zita and Juhász, Gábor and Lukacsovich, David and Winterer, Jochen and Lukacsovich, Tamás and Hegedűs, Zoltán and Kurucz, Judit Éva and Hultmark, Dan and Földy, Csaba and Andó, István}, doi = {10.1159/000520110}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {14}, unique-id = {32524824}, issn = {1662-811X}, year = {2022}, eissn = {1662-8128}, pages = {335-354}, orcid-numbers = {Juhász, Gábor/0000-0001-8548-8874; Winterer, Jochen/0000-0002-6800-6594; Lukacsovich, Tamás/0000-0001-5908-9861; Hultmark, Dan/0000-0002-6506-5855; Andó, István/0000-0002-4648-9396} } @article{MTMT:33181516, title = {Differential immune responses in new and old fruit fly-parasitoid associations: Implications for their management}, url = {https://m2.mtmt.hu/api/publication/33181516}, author = {Gwokyalya, Rehemah and Herren, Jeremy K. K. and Weldon, Christopher W. W. and Khamis, Fathiya M. M. and Ndlela, Shepard and Mohamed, Samira Abuelgasim}, doi = {10.3389/fphys.2022.945370}, journal-iso = {FRONT PHYSIOL}, journal = {FRONTIERS IN PHYSIOLOGY}, volume = {13}, unique-id = {33181516}, abstract = {The oriental fruit fly, Bactrocera dorsalis (Hendel), and marula fruit fly, Ceratitis cosyra (Walker), are major fruit-infesting tephritids across sub-Saharan Africa. Biological control of these pests using parasitic wasps has been widely adopted but with varying levels of success. Most studies investigating host-parasitoid models have focused on functional and evolutionary aspects leaving a knowledge gap about the physiological mechanisms underpinning the efficacy of parasitoids as biocontrol agents of tephritids. To better understand these physiological mechanisms, we investigated changes in the cellular immune responses of C. cosyra and B. dorsalis when exposed to the parasitic wasps, Diachasmimorpha longicaudata (Ashmaed) and Psyttalia cosyrae (Wilkinson). We found that B. dorsalis was more resistant to parasitisation, had a higher hemocyte count, and encapsulated more parasitoid eggs compared to C. cosyra, achieving up to 100% encapsulation when exposed to P. cosyrae. Exposing B. dorsalis to either parasitoid species induced the formation of a rare cell type, the giant multinucleated hemocyte, which was not observed in C. cosyra. Furthermore, compared to P. cosyrae-parasitized larvae, those of both host species parasitized by D. longicaudata had lower encapsulation rates, hemocyte counts and spreading abilities and yielded a higher number of parasitoid progeny with the highest parasitoid emergence (72.13%) recorded in C. cosyra. These results demonstrate that cellular immune responses are central to host-parasitoid interaction in tephritid fruit flies and further suggest that D. longicaudata presents greater potential as a biocontrol agent of B. dorsalis and C. cosyra in horticultural cropping systems.}, keywords = {HEMOCYTES; Immunity; encapsulation; Bactrocera dorsalis; Diachasmimorpha longicaudata; Melanisation; Psytallia cosyrae; Ceratitis cosyra}, year = {2022}, eissn = {1664-042X} } @article{MTMT:33039275, title = {Hematopoietic plasticity mapped in Drosophila and other insects}, url = {https://m2.mtmt.hu/api/publication/33039275}, author = {Hultmark, Dan and Andó, István}, doi = {10.7554/eLife.78906}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {11}, unique-id = {33039275}, issn = {2050-084X}, year = {2022}, eissn = {2050-084X}, orcid-numbers = {Hultmark, Dan/0000-0002-6506-5855; Andó, István/0000-0002-4648-9396} } @article{MTMT:32601764, title = {Characterization and mode of action analysis of black soldier fly (Hermetia illucens) larva-derived hemocytes}, url = {https://m2.mtmt.hu/api/publication/32601764}, author = {von, Bredow Y.M. and Müller, A. and Popp, P.F. and Iliasov, D. and von, Bredow C.-R.}, doi = {10.1111/1744-7917.12977}, journal-iso = {INSECT SCI}, journal = {INSECT SCIENCE}, volume = {29}, unique-id = {32601764}, issn = {1672-9609}, abstract = {With the growing importance of the black soldier fly (Hermetia illucens) for both sustainable food production and waste management as well as for science, a great demand of understanding its immune system arises. Here, we present the first description of the circulating larval hemocytes with special emphasis on uptake of microorganisms and distinguishing hemocyte types. With histological, zymographic, and cytometric methods and with a set of hemocyte binding lectins and antibodies, the hemocytes of H. illucens are identified as plasmatocytes, crystal cells, and putative prohemocytes. Total hemocyte counts (THC) are determined, and methods for THC determination are compared. Approximately 1100 hemocytes per microliter hemolymph are present in naive animals, while hemocyte density decreases dramatically shortly after wounding, indicating a role of hemocytes in response to wounding (and immune response in general). The determination of the relative abundance of each hemocyte type (differential hemocyte count, DHC) revealed that plasmatocytes are highly abundant, whereas prohemocytes and crystal cells make up only a small percentage of the circulating cells. Plasmatocytes are not only the most abundant but also the professional phagocytes in H. illucens. They rapidly engulf and take up bacteria both in vivo and in vitro, indicating a very potent cellular defense against invading pathogens. Larger bioparticles such as yeasts are also removed from circulation by phagocytosis, but slower than bacteria. This is the first analysis of the potent cellular immune response in the black soldier fly, and a first toolbox that helps to identify hemocyte (types) is presented. © 2021 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences}, keywords = {cellular immunity; Plasmatocyte; crystal cell; hemocyte types; black soldier fly (Hermetia illucens); immunity of economically important insects; microbe phagocytosis}, year = {2022}, eissn = {1744-7917}, pages = {1071-1095} } @article{MTMT:32876692, title = {Adaptations and counter-adaptations in Drosophila host–parasitoid interactions: advances in the molecular mechanisms}, url = {https://m2.mtmt.hu/api/publication/32876692}, author = {Wertheim, B.}, doi = {10.1016/j.cois.2022.100896}, journal-iso = {CURR OPIN INSECT SCI}, journal = {CURRENT OPINION IN INSECT SCIENCE}, volume = {51}, unique-id = {32876692}, issn = {2214-5745}, abstract = {Both hosts and parasitoids evolved a diverse array of traits and strategies for their antagonistic interactions, affecting their chances of encounter, attack and survival after parasitoid attack. This review summarizes the recent progress that has been made in elucidating the molecular mechanisms of these adaptations and counter-adaptations in various Drosophila host–parasitoid interactions. For the hosts, it focuses on the neurobiological and genetic control of strategies in Drosophila adults and larvae of avoidance or escape behaviours upon sensing the parasitoids, and the immunological defences involving diverse classes of haemocytes. For the parasitoids, it highlights their behavioural strategies in host finding, as well as the rich variety of venom components that evolved and were partially acquired through horizontal gene transfer. Recent studies revealed the mechanisms by which these venom components manipulate their parasitized hosts in exhibiting escape behaviour to avoid superparasitism, and their counter-strategies to evade or obstruct the hosts’ immunological defences. © 2022 The Author(s)}, year = {2022}, eissn = {2214-5753} } @article{MTMT:31743832, title = {There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells}, url = {https://m2.mtmt.hu/api/publication/31743832}, author = {Csordás, Gábor and Gábor, Erika and Honti, Viktor}, doi = {10.1016/j.ydbio.2020.10.006}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {469}, unique-id = {31743832}, issn = {0012-1606}, year = {2021}, eissn = {1095-564X}, pages = {135-143}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839} } @article{MTMT:32000172, title = {Current concepts in granulomatous immune responses}, url = {https://m2.mtmt.hu/api/publication/32000172}, author = {Herbath, M. and Fabry, Z. and Sandor, M.}, doi = {10.1007/s42977-021-00077-1}, journal-iso = {BIOL FUTURA}, journal = {BIOLOGIA FUTURA}, volume = {72}, unique-id = {32000172}, issn = {2676-8615}, abstract = {Persistent irritants that are resistant to innate and cognate immunity induce granulomas. These macrophage-dominated lesions that partially isolate the healthy tissue from the irritant and the irritant induced inflammation. Particles, toxins, autoantigens and infectious agents can induce granulomas. The corresponding lesions can be protective for the host but they can also cause damage and such damage has been associated with the pathology of more than a hundred human diseases. Recently, multiple molecular mechanisms underlying how normal macrophages transform into granuloma-inducing macrophages have been discovered and new information has been gathered, indicating how these lesions are initiated, spread and regulated. In this review, differences between the innate and cognate granuloma pathways are discussed by summarizing how the dendritic cell-T cell axis changes granulomatous immunity. Granuloma lesions are highly dynamic and depend on continuous cell replacement. This feature provides new therapeutic approaches to treat granulomatous diseases. © 2021, Akadémiai Kiadó Zrt.}, keywords = {DENDRITIC CELLS; granuloma; VEGF; Cell traffic}, year = {2021}, eissn = {2676-8607}, pages = {61-68} } @article{MTMT:31866341, title = {Does Drosophila sechellia escape parasitoid attack by feeding on a toxic resource?}, url = {https://m2.mtmt.hu/api/publication/31866341}, author = {Salazar-Jaramillo, L. and Wertheim, B.}, doi = {10.7717/peerj.10528}, journal-iso = {PEERJ}, journal = {PEERJ}, volume = {9}, unique-id = {31866341}, issn = {2167-8359}, abstract = {Host shifts can drastically change the selective pressures that animals experience from their environment. Drosophila sechellia is a species restricted to the Seychelles islands, where it specializes on the fruit Morinda citrifolia (noni). This fruit is known to be toxic to closely related Drosophila species, including D. melanogaster and D. simulans, releasing D. sechellia from interspecific competition when breeding on this substrate. Previously, we showed that larvae of D. sechellia are unable to mount an effective immunological response against wasp attack, while larvae of closely-related species can defend themselves from parasitoid attack by melanotic encapsulation. We hypothesized that this inability constitutes a trait loss due to a reduced risk of parasitoid attack in noni. Here we present a lab experiment and field survey aimed to test the hypothesis that specialization on noni has released D. sechellia from the antagonistic interaction with its larval parasitoids. Our results from the lab experiment suggest that noni may be harmful to parasitoid wasps. Our results from the field survey indicate that D. sechellia was found in ripe noni, whereas another Drosophila species, D. malerkotliana, was present in unripe and overripe stages. Parasitic wasps of the species Leptopilina boulardi emerged from overripe noni, where D. malerkotliana was the most abundant host, but not from ripe noni. These results indicate that the specialization of D. sechellia on noni has indeed drastically altered its ecological interactions, leading to a relaxation in the selection pressure to maintain parasitoid resistance. Copyright 2021 Salazar-Jaramillo and Wertheim}, keywords = {ARTICLE; Breeding; nonhuman; Parasitism; parasitosis; TOXICITY; feeding; Drosophila melanogaster; encapsulation; leisure; Parasitoid; Parasitoid wasps; melanosis; host shift; Noni; Drosophila sechellia; Drosophila sechellia; Drosophila simulans; trait loss; Drosophila malerkotliana}, year = {2021}, eissn = {2167-8359} } @article{MTMT:31596046, title = {Cellular and humoral immune interactions between Drosophila and its parasitoids}, url = {https://m2.mtmt.hu/api/publication/31596046}, author = {Yang, L. and Qiu, L.-M. and Fang, Q. and Stanley, D.W. and Ye, G.-Y.}, doi = {10.1111/1744-7917.12863}, journal-iso = {INSECT SCI}, journal = {INSECT SCIENCE}, volume = {28}, unique-id = {31596046}, issn = {1672-9609}, abstract = {The immune interactions occurring between parasitoids and their host insects, especially in Drosophila–wasp models, have long been the research focus of insect immunology and parasitology. Parasitoid infestation in Drosophila is counteracted by its multiple natural immune defense systems, which include cellular and humoral immunity. Occurring in the hemocoel, cellular immune responses involve the proliferation, differentiation, migration and spreading of host hemocytes and parasitoid encapsulation by them. Contrastingly, humoral immune responses rely more heavily on melanization and on the Toll, Imd and Jak/Stat immune pathways associated with antimicrobial peptides along with stress factors. On the wasps’ side, successful development is achieved by introducing various virulence factors to counteract immune responses of Drosophila. Some or all of these factors manipulate the host's immunity for successful parasitism. Here we review current knowledge of the cellular and humoral immune interactions between Drosophila and its parasitoids, focusing on the defense mechanisms used by Drosophila and the strategies evolved by parasitic wasps to outwit it. © 2020 Institute of Zoology, Chinese Academy of Sciences}, keywords = {VENOM; DROSOPHILA; Immunity; Parasitoid; VIRUS-LIKE PARTICLES}, year = {2021}, eissn = {1744-7917}, pages = {1208-1227} } @article{MTMT:30819399, title = {Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid Zaprionus indianus}, url = {https://m2.mtmt.hu/api/publication/30819399}, author = {Cinege, Gyöngyi Ilona and Lerner, Zita and Magyar, Lilla Brigitta and Soós, Bálint and Tóth, Renáta and Kristó, Ildikó and Vilmos, Péter and Juhász, Gábor and Kovács, Attila Lajos and Hegedűs, Zoltán and Sensen, Christoph W. and Kurucz, Judit Éva and Andó, István}, doi = {10.1159/000502646}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {12}, unique-id = {30819399}, issn = {1662-811X}, year = {2020}, eissn = {1662-8128}, pages = {257-272}, orcid-numbers = {Juhász, Gábor/0000-0001-8548-8874; Andó, István/0000-0002-4648-9396} } @article{MTMT:31302837, title = {Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes}, url = {https://m2.mtmt.hu/api/publication/31302837}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordás, Gábor and Rusvai, Miklós and Honti, Viktor and Kolics, Balázs and Török, Tibor and Williams, Michael J and Kurucz, Judit Éva and Andó, István}, doi = {10.1016/j.dci.2020.103701}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {109}, unique-id = {31302837}, issn = {0145-305X}, abstract = {Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes.}, keywords = {Immunity; monoclonal antibody; Hemocyte; Apis mellifera; Honey bee; insect immunity}, year = {2020}, eissn = {1879-0089}, pages = {103701-103706}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Török, Tibor/0000-0002-2128-1126; Andó, István/0000-0002-4648-9396} } @article{MTMT:30907275, title = {Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process}, url = {https://m2.mtmt.hu/api/publication/30907275}, author = {Kim-Jo, Chami and Gatti, Jean-Luc and Poirie, Marylene}, doi = {10.3389/fphys.2019.00603}, journal-iso = {FRONT PHYSIOL}, journal = {FRONTIERS IN PHYSIOLOGY}, volume = {10}, unique-id = {30907275}, abstract = {Host-parasitoid interactions are among the most studied interactions between invertebrates because of their fundamental interest - the evolution of original traits in parasitoids - and applied, parasitoids being widely used in biological control. Immunity, and in particular cellular immunity, is central in these interactions, the host encapsulation response being specific for large foreign bodies such as parasitoid eggs. Although already well studied in this species, recent data on Drosophila melanogaster have unquestionably improved knowledge of invertebrate cellular immunity. At the same time, the venomics of parasitoids has expanded, notably those of Drosophila. Here, we summarize and discuss these advances, with a focus on an emerging "time-dependent" view of interactions outcome at the intra- and interspecific level. We also present issues still in debate and prospects for study. Data on the Drosophila-parasitoid model paves the way to new concepts in insect immunity as well as parasitoid wasp strategies to overcome it.}, keywords = {VENOM; DROSOPHILA; Immunity; encapsulation; Hematopoiesis; Parasitoid wasp; Leptopilina}, year = {2019}, eissn = {1664-042X} } @article{MTMT:27048633, title = {Nematocytes: Discovery and characterization of a novel anculeate hemocyte in Drosophila falleni and Drosophila phalerata}, url = {https://m2.mtmt.hu/api/publication/27048633}, author = {Bozler, Julianna and Kacsoh, Balint Z and Bosco, Giovanni}, doi = {10.1371/journal.pone.0188133}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {12}, unique-id = {27048633}, issn = {1932-6203}, year = {2017}, eissn = {1932-6203} } @article{MTMT:3249751, title = {Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes}, url = {https://m2.mtmt.hu/api/publication/3249751}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordás, Gábor and Török, Tibor and Medzihradszky F., Katalin and Darula, Zsuzsanna and Andó, István and Kurucz, Judit Éva}, doi = {10.1016/j.dci.2017.07.013}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {76}, unique-id = {3249751}, issn = {0145-305X}, year = {2017}, eissn = {1879-0089}, pages = {403-411}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Török, Tibor/0000-0002-2128-1126; Andó, István/0000-0002-4648-9396} } @article{MTMT:27048635, title = {Bystander Cells Taking Action}, url = {https://m2.mtmt.hu/api/publication/27048635}, author = {Herwald, Heiko and Egesten, Arne}, doi = {10.1159/000484305}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {9}, unique-id = {27048635}, issn = {1662-811X}, year = {2017}, eissn = {1662-8128}, pages = {527-528} } @article{MTMT:26674068, title = {Inter- and intra-species variation in genome-wide gene expression of Drosophila in response to parasitoid wasp attack}, url = {https://m2.mtmt.hu/api/publication/26674068}, author = {Salazar-Jaramillo, L and Jalvingh, KM and de Haan, A and Kraaijeveld, K and Buermans, H and Wertheim, B}, doi = {10.1186/s12864-017-3697-3}, journal-iso = {BMC GENOMICS}, journal = {BMC GENOMICS}, volume = {18}, unique-id = {26674068}, issn = {1471-2164}, year = {2017}, eissn = {1471-2164} } @article{MTMT:3096913, title = {Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection.}, url = {https://m2.mtmt.hu/api/publication/3096913}, author = {Anderl, I and Vesala, L and Ihalainen, TO and Vanha-Aho, LM and Andó, István and Ramet, M and Hultmark, D}, doi = {10.1371/journal.ppat.1005746}, journal-iso = {PLOS PATHOG}, journal = {PLOS PATHOGENS}, volume = {12}, unique-id = {3096913}, issn = {1553-7366}, abstract = {Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail.}, year = {2016}, eissn = {1553-7374}, pages = {e1005746}, orcid-numbers = {Andó, István/0000-0002-4648-9396} } @article{MTMT:26674070, title = {Bacterial communities differ among drosophila melanogaster populations and affect host resistance against parasitoids}, url = {https://m2.mtmt.hu/api/publication/26674070}, author = {Chaplinska, M and Gerritsma, S and Dini-Andreote, F and Salles, JF and Wertheim, B}, doi = {10.1371/journal.pone.0167726}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {11}, unique-id = {26674070}, issn = {1932-6203}, year = {2016}, eissn = {1932-6203} } @article{MTMT:26376779, title = {Geographic variation and trade-offs in parasitoid virulence}, url = {https://m2.mtmt.hu/api/publication/26376779}, author = {Fors, Lisa and Markus, Robert and Theopold, Ulrich and Ericson, Lars and Hamback, Peter A}, doi = {10.1111/1365-2656.12579}, journal-iso = {J ANIM ECOL}, journal = {JOURNAL OF ANIMAL ECOLOGY}, volume = {85}, unique-id = {26376779}, issn = {0021-8790}, year = {2016}, eissn = {1365-2656}, pages = {1595-1604} } @article{MTMT:26015600, title = {The Origin of a Paradigm}, url = {https://m2.mtmt.hu/api/publication/26015600}, author = {Herwald, Heiko and Egesten, Arne}, doi = {10.1159/000445282}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {8}, unique-id = {26015600}, issn = {1662-811X}, year = {2016}, eissn = {1662-8128}, pages = {221-222} } @article{MTMT:3045263, title = {The raspberry Gene Is Involved in the Regulation of the Cellular Immune Response in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/3045263}, author = {Kari, Beáta and Csordás, Gábor and Honti, Viktor and Cinege, Gyöngyi Ilona and Williams, MJ and Andó, István and Kurucz, Judit Éva}, doi = {10.1371/journal.pone.0150910}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {11}, unique-id = {3045263}, issn = {1932-6203}, abstract = {Drosophila is an extremely useful model organism for understanding how innate immune mechanisms defend against microbes and parasitoids. Large foreign objects trigger a potent cellular immune response in Drosophila larva. In the case of endoparasitoid wasp eggs, this response includes hemocyte proliferation, lamellocyte differentiation and eventual encapsulation of the egg. The encapsulation reaction involves the attachment and spreading of hemocytes around the egg, which requires cytoskeletal rearrangements, changes in adhesion properties and cell shape, as well as melanization of the capsule. Guanine nucleotide metabolism has an essential role in the regulation of pathways necessary for this encapsulation response. Here, we show that the Drosophila inosine 5'-monophosphate dehydrogenase (IMPDH), encoded by raspberry (ras), is centrally important for a proper cellular immune response against eggs from the parasitoid wasp Leptopilina boulardi. Notably, hemocyte attachment to the egg and subsequent melanization of the capsule are deficient in hypomorphic ras mutant larvae, which results in a compromised cellular immune response and increased survival of the parasitoid.}, keywords = {PHAGOCYTOSIS; INHIBITORS; ACTIVATION; SCREEN; RHO; Hematopoiesis; INOSINE MONOPHOSPHATE DEHYDROGENASE; Parasitoids; SMALL GTPASES; LEPTOPILINA-BOULARDI}, year = {2016}, eissn = {1932-6203}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396} } @article{MTMT:26203047, title = {Wright-Giemsa staining to observe phagocytes in Locusta migratoria infected with Metarhizium acridum}, url = {https://m2.mtmt.hu/api/publication/26203047}, author = {Yu, Ying and Cao, Yueqing and Xia, Yuxian and Liu, Feihong}, doi = {10.1016/j.jip.2016.06.009}, journal-iso = {J INVERTEBR PATHOL}, journal = {JOURNAL OF INVERTEBRATE PATHOLOGY}, volume = {139}, unique-id = {26203047}, issn = {0022-2011}, year = {2016}, eissn = {1096-0805}, pages = {19-24} } @article{MTMT:25258801, title = {Drosophila innate immunity: Regional and functional specialization of prophenoloxidases}, url = {https://m2.mtmt.hu/api/publication/25258801}, author = {Dudzic, JP and Kondo, S and Ueda, R and Bergman, CM and Lemaitre, B}, doi = {10.1186/s12915-015-0193-6}, journal-iso = {BMC BIOL}, journal = {BMC BIOLOGY}, volume = {13}, unique-id = {25258801}, issn = {1741-7007}, year = {2015}, eissn = {1741-7007} } @article{MTMT:24982070, title = {Foodies of Innate Immunity}, url = {https://m2.mtmt.hu/api/publication/24982070}, author = {Herwald, H and Egesten, A}, doi = {10.1159/000430800}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {7}, unique-id = {24982070}, issn = {1662-811X}, year = {2015}, eissn = {1662-8128}, pages = {331-332} } @article{MTMT:2993019, title = {Innate immunity}, url = {https://m2.mtmt.hu/api/publication/2993019}, author = {Honti, Viktor and Kurucz, Judit Éva and Cinege, Gyöngyi Ilona and Csordás, Gábor and Andó, István}, journal-iso = {ACTA BIOL SZEGED}, journal = {ACTA BIOLOGICA SZEGEDIENSIS}, volume = {59}, unique-id = {2993019}, issn = {1588-385X}, year = {2015}, eissn = {1588-4082}, pages = {1-15}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396} } @article{MTMT:25139200, title = {Genomic basis of evolutionary change: Evolving immunity}, url = {https://m2.mtmt.hu/api/publication/25139200}, author = {Wertheim, B}, doi = {10.3389/fgene.2015.00222}, journal-iso = {FRONT GENET}, journal = {FRONTIERS IN GENETICS}, volume = {6}, unique-id = {25139200}, year = {2015}, eissn = {1664-8021} }