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Nanocrystalline TiO2, in the form of mixed anatase/rutile phases (P25), is often employed, but must be modified for higher activity in visible light. To improve the light harvesting properties, nano-bio hybrid photocatalysts were prepared by functionalizing P25 with Au-25 clusters, Au nanoparticles, and/or chlorophyll. The most effective photocatalytic degradation of rhodamine B (RhB) was observed over P25 catalysts functionalized with both Au-25 and chlorophyll. HR-TEM, UV-vis, XPS and XANES/EXAFS were applied to examine the factors influencing photocatalytic activity. A direct contact between Au-25, chlorophyll, and P25, strongly improved the electron transfer from chlorophyll to P25, while still maintaining the reusability of catalysts. 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