TY - JOUR AU - Solihat, Nissa Nurfajrin AU - Pramasari, Dwi Ajias AU - Laksana, Raden Permana Budi AU - Restu, Witta Kartika AU - Ghozali, Muhammad AU - Triwulandari, Evi AU - Fatriasari, Widya AU - Watanabe, Takashi TI - Synthesis of lignin-based biosurfactant derived from kraft black liquor and its effect on enzymatic hydrolysis of pretreated biomass JF - SUSTAINABLE CHEMISTRY AND PHARMACY J2 - SUSTAIN CHEM PHARM VL - 34 PY - 2023 PG - 18 SN - 2352-5541 DO - 10.1016/j.scp.2023.101152 UR - https://m2.mtmt.hu/api/publication/34278449 ID - 34278449 AB - Lignin, the main constituent in black liquor of up to 40%, can be converted into a value-added commodity such as a biosurfactant. In this study, lignin was extracted from the Acacia mangium black liquor kraft by acid precipitation (L1S) and ethanol fractionation (L2S). Subsequently, dried lignin was characterized physically and chemically. Biosurfactant was obtained by reacting lignin with polyvinyl alcohol (PVA). To evaluate the fruitfulness of biosurfactants on enzymatic hydrolysis, some optimizations have been conducted: volume ratio PVA: lignin, concentration PVA: lignin, different lignin source, substrate variation, and the effect of an initiator (potassium peroxodisulfate). Lignin by ethanol fractionation resulted in higher lignin content, methoxyl, molecular weight, and syringil but lower particle size and ash content. However, the thermal sta-bility of these two lignins is similar. The ratio of PVA: lignin gave a positive effect on boosting re-ducing sugar yield (RSY) while the high concentration of PVA: lignin had the opposite effect. The best volume of PVA: lignin was 250 & mu;L with a concentration of biosurfactant 0.4% to get the high-est RSY per biomass about 80%. Meanwhile, biosurfactant-based L2S released more RSY than L1S and lignin sigma Aldrich. Kraft process of sweet sorghum bagasse (SSB) obtained more sugar than other substrates such as an alkaline autoclave SSB, jabon soda pulp, and microwave-assisted sul-furic acid pretreated of oil palm empty fruit bunch (EFB). The initiator addition during synthesiz-ing of biosurfactant was predicted to contribute to achieving higher RSY compared to that of that non-initiator addition. This study demonstrates the ability of lignin-based biosurfactants to im-prove enzymatic hydrolysis yet the best condition was depending on the ratio, concentration, and volume of PVA: lignin, lignin types, substrates, and initiator. LA - English DB - MTMT ER - TY - JOUR AU - Wang, J. AU - Xiao, W. AU - Zhang, J. AU - Quan, X. AU - Chu, J. AU - Meng, X. AU - Pu, Y. AU - Ragauskas, A.J. TI - Beneficial effect of surfactant in adsorption/desorption of lignocellulose-degrading enzymes on/from lignin with different structure JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 191 PY - 2023 SN - 0926-6690 DO - 10.1016/j.indcrop.2022.115904 UR - https://m2.mtmt.hu/api/publication/33662094 ID - 33662094 N1 - College of Forestry, Northwest A&F University, Shaanxi, Yangling, 712100, China College of Natural Resources and Environment, Northwest A&F University, Shaanxi, Yangling, 712100, China Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN 37996-2200, United States Joint Institute for Biological Sciences, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, United States Export Date: 24 February 2023 CODEN: ICRDE Correspondence Address: Zhang, J.; College of Forestry, Shaanxi, China; email: junhuazhang@nwafu.edu.cn LA - English DB - MTMT ER - TY - JOUR AU - Zhao, Chenbiao AU - Mai, Shenyi AU - Fan, Meishan AU - Xie, Jun AU - Zhang, Hongdan TI - Effects of Metal Chloride Salt Pretreatment and Additives on Enzymatic Hydrolysis of Poplar JF - FERMENTATION J2 - FERMENTATION-BASEL VL - 9 PY - 2023 IS - 12 PG - 17 SN - 2311-5637 DO - 10.3390/fermentation9121022 UR - https://m2.mtmt.hu/api/publication/34609150 ID - 34609150 AB - Metal chloride salt pretreatment was performed to isolate and convert cellulose to glucose from poplar. A glucose yield of 82.0% +/- 0.7 was achieved after 0.05 mol/L AlCl3 pretreatment conducted at 180 degrees C for 20 min, ascribing to the removal of hemicellulose, the alteration of crystallinity, surface morphology, and the retention of the majority of cellulose. Then, the influence of different additives on glucose yield was assessed, generating the highest glucose yield of 88.5 +/- 0.06 with the addition of PEG 8000. Meanwhile, a similar glucose yield of 82.8% +/- 0.3 could be obtained with PEG 8000 when hydrolysis time was reduced by a quarter and enzyme dosage by three-quarters. It can be seen that AlCl3 pretreatment is a viable and efficient pretreatment method for poplar, while the addition of PEG 8000 can enhance the enzymatic efficiency and reduce cellulase loading, ascribing to the reservation of free enzyme and enzyme activity in the supernatant and the reduction in surface tension, which provide an idea to improve the economics of the enzymatic conversion of poplar. LA - English DB - MTMT ER - TY - JOUR AU - Han, Lijuan AU - Jiang, Baojie AU - Wang, Wei AU - Wang, Gaosheng AU - Tan, Yinshuang AU - Niu, Kangle AU - Fang, Xu TI - Alleviating Nonproductive Adsorption of Lignin on CBM through the Addition of Cationic Additives for Lignocellulosic Hydrolysis JF - ACS APPLIED BIO MATERIALS J2 - ACS APPL BIO MATER VL - 5 PY - 2022 IS - 5 SP - 2253 EP - 2261 PG - 9 SN - 2576-6422 DO - 10.1021/acsabm.2c00112 UR - https://m2.mtmt.hu/api/publication/33003572 ID - 33003572 AB - The nonproductive adsorption of cellulase onto lignin significantly inhibited the enzymatic hydrolysis of lignocellulosic biomass. In this study, we constructed a rapid fluorescence detection (RFD) system, and using this system, we demonstrated that the addition of cationic additives DTAB or polyDADMAC greatly increased the partition coefficients of cellulose/lignin, reduced nonproductive adsorption, and enhanced the hydrolysis efficiency of lignocellulose compared to those of Tweens or PEGs. Moreover, the addition of polyDADMAC and DTAB increased the glucose yield released from the mixture of Avicel and AICS-lignin (MCL) by 16.9 and 20.6%, respectively, and reduced the inhibition rate of lignin by 16.9 and 20.7%, respectively. Interestingly, polyDADMAC or DTAB treatment performed more effectively for the enzymatic hydrolysis of pretreated lignocellulosic biomass, compared with MCL. We confirmed that the reduced hydrophobicity and increased zeta potential of lignin cocontribute to the dampening nonproductive adsorption of lignin. In particular, the zeta potential values of lignin and the partition coefficients of Avicel/lignin with the addition of additives showed a good correlation, suggesting that electrostatic force also plays a crucial role in the adsorbing of cellulase on lignin. This work will be conducive to decreasing the nonproductive binding of cellulase onto lignin and enhancing cellulose conversion. LA - English DB - MTMT ER - TY - JOUR AU - Huang, Caoxing AU - Zhao, Xiaoxue AU - Zheng, Yayue AU - Lin, Wenqian AU - Lai, Chenhuan AU - Yong, Qiang AU - Ragauskas, Arthur J. AU - Meng, Xianzhi TI - Revealing the mechanism of surfactant-promoted enzymatic hydrolysis of dilute acid pretreated bamboo JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 360 PY - 2022 PG - 9 SN - 0960-8524 DO - 10.1016/j.biortech.2022.127524 UR - https://m2.mtmt.hu/api/publication/33224770 ID - 33224770 AB - To improve the enzymatic digestibility of dilute acid pretreated bamboo residue (DABR), surfactants including PEG 4000 and Tween 80 were added to prevent the non-productive adsorption between residual lignin and enzyme. At the optimal loadings (e.g., 0.2 and 0.3 g surfactant/g lignin), the enzymatic digestibility of DABR improved from 29.4% to 64.6% and 61.6% for PEG 4000 and Tween 80, respectively. Furthermore, the pro-moting mechanism of these surfactants on enzymatic hydrolysis was investigated by real-time surface plasmon resonance (SPR) and fluorescence spectroscopy. Results from SPR analysis showed that Tween 80 outperformed PEG 4000 in terms of dissociating the irreversible cellulase adsorption onto lignin. Fluorescence quenching mechanism revealed that PEG 4000 and Tween 80 intervened the interaction between lignin and cellulase by hydrogen bonds/Van der Waals and hydrophobic action, respectively. This work provided an in-depth under-standing of the mechanisms of PEG 4000 and Tween 80 on enhancing the enzymatic hydrolysis efficiency. LA - English DB - MTMT ER - TY - JOUR AU - Li, Mohan AU - Jiang, Bo AU - Wu, Wenjuan AU - Wu, Shufang AU - Yang, Yiqin AU - Song, Junlong AU - Ahmad, Mehraj AU - Jin, Yongcan TI - Current understanding and optimization strategies for efficient lignin-enzyme interaction: A review JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 195 PY - 2022 SP - 274 EP - 286 PG - 13 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2021.11.188 UR - https://m2.mtmt.hu/api/publication/33003575 ID - 33003575 AB - From energy perspective, with abundant polysaccharides (45-85%), the renewable lignocellulosic is recognized as the 2nd generation feedstock for bioethanol and bio-based products production. Enzymatic hydrolysis is a critical pathway to yield fermentable monosaccharides from pretreated substrates of lignocellulose. Nevertheless, the lignin presence in lignocellulosic substrates leads to the low substrate enzymatic digestibility ascribed to the nonproductive adsorption. It has been reported that the water-soluble lignin (low molecular weight, sulfonated/ sulfomethylated and graft polymer) enhance the rate of enzymatic digestibility, however, the catalytic mechanism of lignin-enzyme interaction remains elusive. In this review, optimization strategies for enzymatic hydrolysis based on the lignin structural modification, enzyme engineering, and different additives are critically reviewed. Lignin-enzyme interaction mechanism is also discussed (lignin and various cellulases). In addition, the mathematical models and simulation of lignin, cellulose and enzyme aims for promoting an integrated biomassconversion process for sustainable production of value-added biofuels. LA - English DB - MTMT ER - TY - JOUR AU - Nogueira, Cleitiane da Costa AU - de Araujo Padilha, Carlos Eduardo AU - de Souza Filho, Pedro Ferreira AU - dos Santos, Everaldo Silvino TI - Effects of the Addition of Poly(ethylene Glycol) and Non-ionic Surfactants on Pretreatment, Enzymatic Hydrolysis, and Ethanol Fermentation JF - BIOENERGY RESEARCH J2 - BIOENERG RES VL - 15 PY - 2022 IS - 2 SP - 889 EP - 904 PG - 16 SN - 1939-1234 DO - 10.1007/s12155-021-10388-9 UR - https://m2.mtmt.hu/api/publication/33003573 ID - 33003573 AB - The consolidation of cellulosic ethanol on the market is fundamental to mitigate the consumption of fuels from fossil sources and to reduce the impact caused by the large generation of agro-industrial waste. In order to achieve this objective, some challenges of cellulosic ethanol technology must be overcome, including the improvement of the cellulosic ethanol production steps. Several studies propose the use of poly(ethylene glycol) (PEG) and non-ionic surfactants (such as Tween 80, Tween 20, and Triton X-100) as a way to increase cellulosic ethanol titers. The benefits attributed to the PEG and non-ionic surfactants go beyond the increase of the concentration of free cellulases during enzymatic hydrolysis. Successful cases of pretreatments of lignocellulosic biomasses assisted by PEG and surfactants and the detoxification of inhibitor-rich hydrolysates with PEG reveal the existence of a plethora of positive mechanisms. Therefore, the present review article is focused on the benefits and mechanisms involved in the addition of PEG and non-ionic surfactants in the pretreatment, enzymatic hydrolysis, and ethanol fermentation steps. Interactions between additives and lignin as well as schemes based on high PEG concentrations were also discussed in detail. LA - English DB - MTMT ER - TY - JOUR AU - Prabmark, Kanoknart AU - Boonyapakron, Katewadee AU - Bunterngsook, Benjarat AU - Arunrattanamook, Nattapol AU - Uengwetwanit, Tanaporn AU - Chitnumsub, Penchit AU - Champreda, Verawat TI - Enhancement of catalytic activity and alkaline stability of cellobiohydrolase by structure-based protein engineering JF - 3 BIOTECH J2 - 3 BIOTECH VL - 12 PY - 2022 IS - 10 PG - 14 SN - 2190-572X DO - 10.1007/s13205-022-03339-4 UR - https://m2.mtmt.hu/api/publication/33224769 ID - 33224769 AB - Alkaline cellobiohydrolases have the potential for application in various industries, including pulp processing and laundry where operation under high pH conditions is preferred. In this study, variants of CtCel6A cellobiohydrolase from Chaetomium thermophilum were generated by structural-based protein engineering with the rationale of increasing catalytic activity and alkaline stability. The variants included removal of the carbohydrate-binding module (CBM) and substitution of residues 173 and 200. The CBM-deleted enzyme with Y200F mutation predicted to mediate conformational change at the N-terminal loop demonstrated increased alkaline stability at 60 degrees C, pH 8.0 for 24 h up to 2.25-fold compared with the wild-type enzyme. Another CBM-deleted enzyme with L173E mutation predicted to induce a new hydrogen bond in the substrate-binding cleft showed enhanced hydrolysis yield of pretreated sugarcane trash up to 4.65-fold greater than that of the wild-type enzyme at the pH 8.0. The variant enzymes could thus be developed for applications on cellulose hydrolysis and plant fiber modification operated under alkaline conditions. LA - English DB - MTMT ER - TY - JOUR AU - Almeida, Renata M. R. G. AU - Pimentel, Wagner R. O. AU - Santos-Rocha, Martha S. R. AU - Buffo, Mariane M. AU - Farinas, Cristiane Sanchez AU - Ximenes, Eduardo A. AU - Ladisch, Michael R. TI - Protective effects of non-catalytic proteins on endoglucanase activity at air and lignin interfaces JF - BIOTECHNOLOGY PROGRESS J2 - BIOTECHNOL PROGR VL - 37 PY - 2021 IS - 4 PG - 12 SN - 8756-7938 DO - 10.1002/btpr.3134 UR - https://m2.mtmt.hu/api/publication/32391978 ID - 32391978 AB - The manner in which added non-catalytic proteins during enzymatic hydrolysis of lignocellulosic substrates enhances hydrolysis mechanisms is not completely understood. Prior research has indicated that a reduction in the non-specific adsorption of enzymes on lignin, and deactivation of enzymes exposed to air-liquid interface provide rationale. This work investigated root causes including effects of the air-liquid interface on non-catalytic proteins, and effects of lignin on endoglucanase. Three different experimental designs and three variables (air-liquid interfacial area, the types of lignin (acid or enzymatic lignin), and the presence of non-enzymatic protein (bovine serum albumin [BSA] or soy proteins ) were used. The results showed that acid isolated lignin adsorbed almost all endoglucanase activity initially present in supernatant, independent of air interface conditions (25 or 250 ml flasks) with the presence of BSA preventing this effect. Endoglucanase lost 30%-50% of its activity due to an air-liquid interface in the presence of lignin while addition of non-enzymatic protein helped to preserve this enzyme's activity. Langmuir and Freundlich models applied to experimental data indicated that the adsorption increases with increasing temperature for both endoglucanase and BSA. Adsorption of the enzyme and protein were endothermic with an increase in entropy. These results, combined, show that hydrophobicity plays a strong role in the adsorption of both endoglucanase and BSA on lignin. LA - English DB - MTMT ER - TY - JOUR AU - Costa, Carolina AU - Medronho, Bruno AU - Eivazi, Alireza AU - Svanedal, Ida AU - Lindman, Bjorn AU - Edlund, Hakan AU - Norgren, Magnus TI - Lignin enhances cellulose dissolution in cold alkali JF - CARBOHYDRATE POLYMERS J2 - CARBOHYD POLYM VL - 274 PY - 2021 PG - 8 SN - 0144-8617 DO - 10.1016/j.carbpol.2021.118661 UR - https://m2.mtmt.hu/api/publication/32391974 ID - 32391974 AB - Aqueous sodium hydroxide solutions are extensively used as solvents for lignin in kraft pulping. These are also appealing systems for cellulose dissolution due to their inexpensiveness, ease to recycle and low toxicity. Cellulose dissolution occurs in a narrow concentration region and at low temperatures. Dissolution is often incomplete but additives, such as zinc oxide or urea, have been found to significantly improve cellulose dissolution. In this work, lignin was explored as a possible beneficial additive for cellulose dissolution. Lignin was found to improve cellulose dissolution in cold alkali, extending the NaOH concentration range to lower values. The regenerated cellulose material from the NaOH-lignin solvents was found to have a lower crystallinity and crystallite size than the samples prepared in the neat NaOH and NaOH-urea solvents. Beneficial lignin-cellulose interactions in solution state appear to be preserved under coagulation and regeneration, reducing the tendency of crystallization of cellulose. LA - English DB - MTMT ER - TY - JOUR AU - Gomaa, Mohamed A. AU - Al-Makhmari, Moza AU - Al-Hinai, Mohab Ali TI - Comparing bioethanol production using buttonwood (Conocarpus erectus) and date palm (Phoenix dactylifera) leaves as raw material JF - BIOFUELS J2 - BIOFUELS-UK VL - 12 PY - 2021 IS - 7 SP - 769 EP - 775 PG - 7 SN - 1759-7269 DO - 10.1080/17597269.2018.1537207 UR - https://m2.mtmt.hu/api/publication/32391977 ID - 32391977 AB - A major obstacle of bioethanol production is finding a renewable and cheap feedstock, which still needs to be addressed. This work focuses on comparing bioethanol production from buttonwood and date palm leaves. Furthermore, cellulase activities were compared from 4 newly isolated cellulase producing Bacillus spp. and optimal temperature, pH and metal ion supplementation conditions to enhance the cellulolytic activity were determined. Finally, the bioethanol yields were compared between a Clostridium aciditolerans sp. (hereafter referred to as AK-1) and Saccharomyces cerevisiae for maximum bioethanol yields. Cellulase activity as high as 372 and 192 U/g substrate were obtained using buttonwood and date palm leaves, respectively, under optimal conditions. Using newly isolated Bacillus strain MI-42 for saccharification, efficiencies of 77.8 and 72.5% were obtained from buttonwood and date palm leaves, respectively. Using AK-1, maximum bioethanol yields of 2.07 and 0.85 g/L were obtained from buttonwood and date palm leaves, respectively, while maximum yields using S. cerevisiae reached 2.12 and 0.9 g/L. However, AK-1 required 96 hours to reach maximum yield, while S. cerevisiae needed just 10 hours. Although buttonwood and date palm leaves could be suitable feedstock, further optimization of the fermentation process is still needed to improve bioethanol yields. LA - English DB - MTMT ER - TY - JOUR AU - Gong, Zhenggang AU - Yang, Guangxu AU - Song, Junlong AU - Zheng, Peitao AU - Liu, Jing AU - Zhu, Wenyuan AU - Huang, Liulian AU - Chen, Lihui AU - Luo, Xiaolin AU - Shuai, Li TI - Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses JF - BIORESOURCES AND BIOPROCESSING J2 - BIORES BIOPROC VL - 8 PY - 2021 IS - 1 PG - 14 SN - 2197-4365 DO - 10.1186/s40643-021-00363-9 UR - https://m2.mtmt.hu/api/publication/32391982 ID - 32391982 AB - Lignin deposits formed on the surface of pretreated lignocellulosic substrates during acidic pretreatments can non-productively adsorb costly enzymes and thereby influence the enzymatic hydrolysis efficiency of cellulose. In this article, peanut protein (PP), a biocompatible non-catalytic protein, was separated from defatted peanut flour (DPF) as a lignin blocking additive to overcome this adverse effect. With the addition of 2.5 g/L PP in enzymatic hydrolysis medium, the glucose yield of the bamboo substrate pretreated by phenylsulfonic acid (PSA) significantly increased from 38 to 94% at a low cellulase loading of 5 FPU/g glucan while achieving a similar glucose yield required a cellulase loading of 17.5 FPU/g glucan without PP addition. Similar promotion effects were also observed on the n-pentanol-pretreated bamboo and PSA-pretreated eucalyptus substrates. The promoting effect of PP on enzymatic hydrolysis was ascribed to blocking lignin deposits via hydrophobic and/or hydrogen-bonding interactions, which significantly reduced the non-productive adsorption of cellulase onto PSA lignin. Meanwhile, PP extraction also facilitated the utilization of residual DPF as the adhesive for producing plywood as compared to that without protein pre-extraction. This scheme provides a sustainable and viable way to improve the value of woody and agriculture biomass. Peanut protein, a biocompatible non-catalytic protein, can block lignin, improve enzymatic hydrolysis efficiency and thereby facilitate the economics of biorefinery. LA - English DB - MTMT ER - TY - JOUR AU - Lv, Xianqing AU - Yang, Guangxu AU - Gong, Zhenggang AU - Cheng, Xin AU - Shuai, Li AU - Huang, Liulian AU - Chen, Lihui AU - Luo, Xiaolin AU - Liu, Jing TI - Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo JF - FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY J2 - FRONT BIOENG BIOTECHNOL VL - 9 PY - 2021 PG - 13 SN - 2296-4185 DO - 10.3389/fbioe.2021.804456 UR - https://m2.mtmt.hu/api/publication/33003576 ID - 33003576 AB - Chemical pretreatment followed by enzymatic hydrolysis has been regarded as a viable way to produce fermentable sugars. Phenylsulfonic acid (PSA) pretreatment could efficiently fractionate the non-cellulosic components (hemicelluloses and lignin) from bamboo and result in increased cellulose accessibility that was 10 times that of untreated bamboo. However, deposited lignin could trigger non-productive adsorption to enzymes, which therefore significantly decreased the enzymatic hydrolysis efficiency of PSA-pretreated bamboo substrates. Herein, poly(N-vinylcaprolactam) (PNVCL), a non-ionic surfactant, was developed as a novel additive for overcoming the non-productive adsorption of lignin during enzymatic hydrolysis. PNVCL was found to be not only more effective than those of commonly used lignosulfonate and polyvinyl alcohol for overcoming the negative effect of lignin, but also comparable to the robust Tween 20 and bovine serum albumin additives. A PNVCL loading at 1.2 g/L during enzymatic hydrolysis of PSA pretreated bamboo substrate could achieve an 80% cellulosic enzymatic conversion and meanwhile reduce the cellulase loading by three times as compared to that without additive. Mechanistic investigations indicated that PNVCL could block lignin residues through hydrophobic interactions and the resultant PNVCL coating resisted the adsorption of cellulase via electrostatic repulsion and/or hydration. This practical method can improve the lignocellulosic enzymatic hydrolysis efficiency and thereby increase the productivity and profitability of biorefinery. LA - English DB - MTMT ER - TY - JOUR AU - Mou, Hongyan AU - Wu, Xiao AU - Huang, Jin AU - Liu, Yibei AU - Fan, Huiming TI - Eucalyptus lignin modification for dynamic adsorption with lignocellulose-degradation enzymes dependent on pH values JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 169 PY - 2021 PG - 9 SN - 0926-6690 DO - 10.1016/j.indcrop.2021.113650 UR - https://m2.mtmt.hu/api/publication/32391980 ID - 32391980 AB - The large amount of ineffective adsorption of cellulase on lignin seriously impeded the enzymatic hydrolysis efficiency and reduced the enzyme activity. In this study, the eucalyptus lignin obtained by alkali and hydrotropic methods was used as raw materials to investigate the differences in the dynamic adsorption with different enzymes and enzyme components in detail. The lignin influenced on enzyme activity of different enzymes and enzyme components was determined as well. Furthermore, alkali lignin (AL) and sodium xylene sulfonate lignin (SL) were modified by dimethylamine (DMA) and diethylenetriamine (DETA) through Mannich reaction. By introducing a certain amount of amine groups on lignin to reduce the surface charge and hydrophobicity of lignin for greatly reduction adsorption of cellulase. Under the optimum condition (pH 4.8), the adsorption of cellulase onto DETA-AL-amine was reduced by 96.32 % from 136 mg g-1 to 5 mg g-1. This work suggests that ligninamine has potential applications in reducing enzyme adsorption. LA - English DB - MTMT ER - TY - JOUR AU - Nogueira, Cleitiane da Costa AU - Padilha, Carlos Eduardo de Araujo AU - dos Santos, Everaldo Silvino TI - Boosting second-generation ethanol titers from green coconut fiber by using high-concentration polyethylene glycol JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 166 PY - 2021 PG - 10 SN - 0926-6690 DO - 10.1016/j.indcrop.2021.113494 UR - https://m2.mtmt.hu/api/publication/32323125 ID - 32323125 N1 - Laboratory of Biochemical Engineering, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil Laboratory of Alternative Energy and Transport Phenomena, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil Cited By :4 Export Date: 14 October 2022 CODEN: ICRDE Correspondence Address: Padilha, C.E.D.A.; Laboratory of Biochemical Engineering, Brazil; email: carlospadilha.eq@gmail.com Funding details: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES Funding details: Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, 141275/2017-0 Funding text 1: The authors thank the Coordination for the Improvement of Higher Education Personnel (CAPES) and the Brazilian National Council of Research (CNPq) for the financial support (Grant number: 141275/2017-0 ). AB - Efforts have been made to improve and modify lignocellulosic pretreatments, but this step corresponds to a high cost in second-generation ethanol production. Previous studies have shown that high-concentration polyethylene glycol (PEG) is a possible way to dispense chemical and physicochemical pretreatments and increase ethanol production from lignocellulosic material. However, no operational conditions of this strategy were optimized. The present study investigated the effects of operational conditions on simultaneous saccharification and fermentation (SSF) performance using high PEG concentrations and green coconut fiber (GCF) as a substrate. The high-concentration PEG (150 g.L-1) increased ethanol production compared to the PEG-free, 10, and 50 g.L-1 PEG conditions. Using 20 % (w.v- 1) solid loading, the batch SSF cultivation with PEG 1500 and without supplementary nutrients reached ethanol production and ethanol yield equal to 22.21 g.L-1 and 64.0 %, respectively. The batch SSF cultivation with 30 % (w.v- 1) solid loading presented mass transfer limitations. The addition of salts, yeast extract, or peptone improved ethanol production. Inhibition by ethanol on the Saccharomyces cerevisiae CAT-1 strain was indifferent to the presence of PEG 1500, while the addition of PEG ensured the cellulolytic activity reuse after three fermentation cycles. The fed-batch SSF with PEG facilitated the GCF liquefaction so that it was possible to operate with up to 30 % (w.v- 1) solid loading without problems of viscosity and free water. Using 150 g.L-1 PEG 1500 and enzyme loading equal to 13.3 filter paper unit. g-1, the fed-batch SSF reached 35.1 g.L-1 ethanol at 48 h, representing an increase of 70 % compared to the fed-batch SSF without PEG. LA - English DB - MTMT ER - TY - JOUR AU - Nogueira, Cleitiane da Costa AU - de Araujo Padilha, Carlos Eduardo AU - dos Santos, Everaldo Silvino TI - Enzymatic hydrolysis and simultaneous saccharification and fermentation of green coconut fiber under high concentrations of ethylene oxide-based polymers JF - RENEWABLE ENERGY J2 - RENEW ENERGY VL - 163 PY - 2021 SP - 1536 EP - 1547 PG - 12 SN - 0960-1481 DO - 10.1016/j.renene.2020.10.050 UR - https://m2.mtmt.hu/api/publication/32391979 ID - 32391979 AB - The present study focuses on elucidating the effects of ethylene oxide-based polymers on cellulosic ethanol production from green coconut fiber (GCF). Data on ethanol production, cell viability, adsorption of cellulases, and enzymatic digestibility were collected from experiments with high concentrations of PEG 4000 and poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) 5800 (EOPO 5800). PEG 4000 and EOPO 5800 favored cell viability and ethanol production in simulated experiments with S. cerevisiae CAT-1 and Kluyveromyces marxianus. Enzymatic hydrolysis under 175 g L-1 PEG 4000 increased the sugar release using untreated GCF (702.0%), hydrothermally-pretreated GCF (63.2%), and acid-pretreated GCF (40.1%). However, there were no benefits to using SigmaCell cellulose as the substrate. EOPO 5800 at 100 g L-1 had positive effects on cellulolytic activity preservation and on the sugar release during enzymatic hydrolysis. Both polymers provided higher ethanol titers for simultaneous saccharification and fermentation (SSF) of pretreated GCF compared to polymer-free experiments. A high ethanol yield (89.8%) was reached by the SSF of untreated GCF using 175 g L-1 PEG 4000. This result implies that high polymer concentrations may enable the cellulosic ethanol scheme without the need for pretreatment. (C) 2020 Elsevier Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Nogueira, Cleitiane da Costa AU - Padilha, Carlos Eduardo de Araujo AU - Dantas, Julia Maria de Medeiros AU - de Medeiros, Fabio Gonsalves Macedo AU - Guilherme, Alexandre de Araujo AU - Souza, Domingos Fabiano de Santana AU - dos Santos, Everaldo Silvino TI - In-situ detoxification strategies to boost bioalcohol production from lignocellulosic biomass JF - RENEWABLE ENERGY J2 - RENEW ENERGY VL - 180 PY - 2021 SP - 914 EP - 936 PG - 23 SN - 0960-1481 DO - 10.1016/j.renene.2021.09.012 UR - https://m2.mtmt.hu/api/publication/32391975 ID - 32391975 AB - The bioalcohol production (ethanol and butanol) from lignocellulosic biomass has some technoeconomic limitations nowadays. Detoxification technologies can efficiently remove fermentation inhibitors (such as furan aldehydes, aliphatic acids, and phenolic compounds) to increase the bioalcohol titers. However, the addition of more steps before fermentation increases the complexity of the production scheme and reduces the profit margin. Process intensification concepts have been disseminated as a critical factor for the success of biotechnological processes, including bioalcohol production. Thus, this review discusses the main inhibitors from lignocellulosic biomass and the in-situ technologies used to minimize the yeast inhibition in hydrolysates and slurries. In-situ detoxification strategies involve different fundamentals, but they have the unanimity of allowing fermentation to be carried out concurrently. They are usually based on the establishment of a microbial consortium as well as the addition of reducing agents, polymers, solvents and adsorbents. Also, in-situ product recovery methods have been developed to minimize energy consumption in downstream processing. (c) 2021 Elsevier Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Nogueira, Cleitiane da Costa AU - Padilha, Carlos Eduardo de Araujo AU - Gilherme, Alexande de Araujo AU - de Souza, Domingos Fabiano Santana AU - de Oliveira, Jackson Araujo AU - dos Santos, Everaldo Silvino TI - Strategies for the Cellulosic Ethanol Production by Using High-Concentration Poly(ethylene glycol) in the Pretreatment, Enzymatic Hydrolysis, and Fermentation Steps JF - BIOENERGY RESEARCH J2 - BIOENERG RES PY - 2021 PG - 14 SN - 1939-1234 DO - 10.1007/s12155-021-10306-z UR - https://m2.mtmt.hu/api/publication/32323123 ID - 32323123 N1 - Laboratory of Biochemical Engineering, Chemical Engineering Department, Federal University of Rio Grande Do Norte (UFRN), RN, Natal, Brazil Laboratory of Alternative Energy and Transport Phenomena, Chemical Engineering Department, Federal University of Rio Grande Do Norte (UFRN), RN, Natal, Brazil Cited By :2 Export Date: 14 October 2022 Correspondence Address: dos Santos, E.S.; Laboratory of Biochemical Engineering, RN, Brazil; email: everaldo@eq.ufrn.br Funding details: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES Funding details: Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, 141275/2017–0 Funding text 1: The authors received financial support from Coordination for the Improvement of Higher Education Personnel (CAPES) and the Brazilian National Council of Research (CNPq) (Grants: 141275/2017–0). AB - The addition of poly(ethylene glycol) (PEG) in the pretreatment and enzymatic hydrolysis steps has become attractive to increase second-generation ethanol titers. Recent studies have shown the benefits of using high-concentration PEG on the yeast vitality and enzymatic digestibility of untreated biomass. Thus, this study investigated the ideal concentration of PEG in the second-generation ethanol production and the best step to add it. In the acid pretreatment, PEG 4000 favored the delignification (59.2%) and reduced the non-productive adsorption of cellulases onto corn cob, but its use was not significant on the sodium carbonate-based pretreatment. Changes in PEG dosage and PEG molecular weight strongly affected the performance of enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF). PEG 4000 (150 g/L) increased the production of reducing sugars by up to 30.1% and the ethanol production via SSF by up to 44.7% from untreated corn cob. In turn, PEG slightly affected the enzymatic digestibility and fermentability of the pretreated corn cob. One-pot ethanol production from corn cob was performed using PEG as an additive. The addition of 150 g/L PEG 1500 and PEG 4000 increased the ethanol yield values (> 52%) in the one-pot processes; however, the ethanol concentration was lower than in the experiments with the pretreated corn cob. SSF experiments with high solid loadings were tested using PEG 1500. Under these conditions, PEG 1500 slightly increased ethanol production, from 53.4 to 55.7 g/L ethanol (equivalent to 92.8% ethanol yield) using 20% (w/v) acid-pretreated corn cob. LA - English DB - MTMT ER - TY - JOUR AU - Padilha, Carlos Eduardo de Araujo AU - Nogueira, Cleitiane da Costa AU - Alencar, Barbara Ribeiro Alves AU - de Abreu, Ithalo Barbosa Silva AU - Dutra, Emmanuel Damilano AU - Ruiz, Juan Alberto Chavez AU - Souza, Domingos Fabiano de Santana AU - dos Santos, Everaldo Silvino TI - Production and Application of Lignin-Based Chemicals and Materials in the Cellulosic Ethanol Production: An Overview on Lignin Closed-Loop Biorefinery Approaches JF - WASTE AND BIOMASS VALORIZATION J2 - WASTE BIOMASS VALORI VL - 12 PY - 2021 SP - 6309 EP - 6337 PG - 29 SN - 1877-2641 DO - 10.1007/s12649-021-01455-5 UR - https://m2.mtmt.hu/api/publication/32391981 ID - 32391981 AB - Lignocellulosic biomass is the most abundant biological resource on the planet and has been extensively researched to produce cellulosic ethanol. However, there is a consensus that the presence of lignin hinders the biomass conversion. Lignin is often considered a villain in cellulosic ethanol production studies due to its adverse effects on cellulases and yeasts. Despite this, recent studies indicate that lignins can be transformed into useful inputs to produce cellulosic ethanol. These approaches aim to establish closed-loop biorefineries to improve economic metrics and reduce the environmental impact due to the substitution of products based on fossil sources. The present review addresses the successful cases in transforming lignin into chemicals and materials to increase cellulosic ethanol titers. A contextualization was first carried out, considering aspects of biomass characteristics and lignin valorization. The impact of lignin-based chemicals and materials in the pretreatment, detoxification, and enzymatic hydrolysis steps was discussed in detail. Economic aspects and future perspectives were also included in this review. These reports open a new point of view on lignin valorization and its integration with the cellulosic ethanol production chain.[GRAPHICS]. LA - English DB - MTMT ER - TY - JOUR AU - Park, Sohyun AU - Yoon, Sae-Min AU - Kim, Yeong-suk AU - Kim, Young-Kyoon AU - Yun, Jeonghee TI - Optimization of Culture Conditions for Cellulase Production in Acanthophysium Sp. KMF001 Using Response Surface Methodology JF - BIORESOURCES J2 - BIORESOURCES VL - 16 PY - 2021 IS - 2 SP - 3520 EP - 3542 PG - 23 SN - 1930-2126 DO - 10.15376/biores.16.2.3520-3542 UR - https://m2.mtmt.hu/api/publication/32391976 ID - 32391976 AB - There are differences in the extracellular enzymes produced from species of wood-rotting fungi and their activity due to variation in nutritional conditions such as carbon sources and nitrogen sources, as well as environmental conditions such as incubation temperature and pH. In this study, to determine the methods to promote the secretion of especially cellulase with high activity among the extracellular enzymes of wood-damaging fungi, the optimal nutrient sources and incubation conditions for the production of cellulase with high activity were investigated using response surface methodology based on a broth culture from Acanthophysium sp. KMF001, a novel strain of wood-damaging fungus. The nutrient sources that were optimal for the cellulase production with high activity from Acanthophysium sp. KMF001 were cellulose as a carbon source and tryptone:yeast extract (7:3) as a nitrogen source. The optimal incubation conditions were a temperature of 30 degrees C and a pH of 6. The optimal concentrations of carbon and nitrogen sources were cellulose at 31.1 g.L-1 and tryptone:yeast extract (7:3) at 15 g.L-1, with pH of 5.9. LA - English DB - MTMT ER - TY - JOUR AU - Park, Sohyun AU - Yang, Jae-Kyung AU - Kim, Young-Kyoon AU - Yun, Jeonghee AU - Kim, Yeong-suk TI - Optimizing the Saccharification of Pretreated Wood Biomass using Crude Enzyme from Acanthophysium Sp. KMF001 JF - BIORESOURCES J2 - BIORESOURCES VL - 16 PY - 2021 IS - 1 SP - 1207 EP - 1229 PG - 23 SN - 1930-2126 DO - 10.15376/biores.16.1.1207-1229 UR - https://m2.mtmt.hu/api/publication/32323400 ID - 32323400 AB - This study was conducted on crude enzyme from the novel strain Acanthophysium sp. KMF001 using a mediator (surfactant). The surfactant was applied to the steam-exploded pretreated domestic wood biomass, and response surface methodology (RSM) was conducted to determine the optimum conditions for saccharification using the optimum substrate concentration, enzyme concentration, and surfactant concentration. Steam-explosion of Korean oak (25 kgf/cm(2)) for 7 min showed a maximum-predicted saccharification of approximately 99.9% at 7.0% substrate concentration, 37.5 FPU (filter paper units) enzyme concentration, and 475.8 mg/g-glucan surfactant (polysorbate 80) concentration. Steam-explosion of red pine (25 kgf/cm(2)) for 7 min revealed a maximum prediction saccharification rate of approximately 58.7% at 6.5% substrate concentration, 36.3 FPU enzyme concentration, and 330.3 mg/g-glucan surfactant (polysorbate 80) concentration. The extents of saccharification of Korean oak (99.9%) and pine (58.7%) demonstrated the high applicability of the crude enzyme from Acanthophysium sp. KMF001. LA - English DB - MTMT ER - TY - JOUR AU - Pihlajaniemi, V. AU - Kallioinen, A. AU - Sipponen, M.H. AU - Nyyssölä, A. TI - Modeling and optimization of polyethylene glycol (PEG) addition for cost-efficient enzymatic hydrolysis of lignocellulose JF - BIOCHEMICAL ENGINEERING JOURNAL J2 - BIOCHEM ENG J VL - 167 PY - 2021 SN - 1369-703X DO - 10.1016/j.bej.2020.107894 UR - https://m2.mtmt.hu/api/publication/31844457 ID - 31844457 N1 - Export Date: 1 February 2021 CODEN: BEJOF Correspondence Address: Pihlajaniemi, V.email: ville.pihlajaniemi@vtt.fi LA - English DB - MTMT ER - TY - JOUR AU - Tokin, Radina AU - Ipsen, Johan Orskov AU - Poojary, Mahesha M. AU - Jensen, Poul Erik AU - Olsson, Lisbeth AU - Johansen, Katja Salomon TI - Inhibition of LPMOs by Fermented Persimmon Juice JF - BIOMOLECULES J2 - BIOMOLECULES VL - 11 PY - 2021 IS - 12 PG - 12 SN - 2218-273X DO - 10.3390/biom11121890 UR - https://m2.mtmt.hu/api/publication/33003574 ID - 33003574 AB - Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes. The inhibitory effect of Kakishibu on lytic polysaccharide monooxygenases (LPMOs) and on a cocktail of cellulose hydrolases was studied, using three different cellulosic substrates. Dose dependent inhibition of LPMO activity by a commercial Kakishibu product was assessed for the well-characterized LPMO from Thermoascus aurantiacus TaAA9A, and the inhibitory effect was confirmed on five additional microbial LPMOs. The model tannin compound, tannic acid exhibited a similar inhibitory effect on TaAA9A as Kakishibu. It was further shown that both polyethylene glycol and tannase can alleviate the inhibitory effect of Kakishibu and tannic acid, indicating a likely mechanism of inhibition caused by unspecific tannin-protein interactions. LA - English DB - MTMT ER - TY - JOUR AU - Zhao, Xiaoshen AU - Liu, Liyang AU - Deng, Zujun AU - Liu, Shan AU - Yun, Jeonyun AU - Xiao, Xiong AU - Li, He TI - Screening, cloning, enzymatic properties of a novel thermostable cellulase enzyme, and its potential application on water hyacinth utilization JF - INTERNATIONAL MICROBIOLOGY J2 - INT MICROBIOL VL - 24 PY - 2021 IS - 3 SP - 337 EP - 349 PG - 13 SN - 1139-6709 DO - 10.1007/s10123-021-00170-4 UR - https://m2.mtmt.hu/api/publication/32323119 ID - 32323119 N1 - School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou, 511400, China Guangdong Key Laboratory of Bioactive Drug Research, Guangzhou, 511400, China School of Business Medicine, Guangdong Pharmaceutical University, Guangzhou, 511400, China Guangzhou Basic Clean Cosmetics Manufacturing Co., Ltd, Guangzhou, 510080, China Cited By :6 Export Date: 14 October 2022 Correspondence Address: Li, H.; Guangdong Key Laboratory of Bioactive Drug ResearchChina; email: lihe32@163.com Chemicals/CAS: cellulase, 9012-54-8; cellulose, 61991-22-8, 68073-05-2, 9004-34-6; Cellulase; Cellulose Funding text 1: This work was financially supported by the Guangzhou Basic Clean Cosmetics Manufacturing Co. Ltd. AB - Cellulose is the cheapest, natural, renewable organic substance that is used as a carbon source in various fields. Water hyacinth, an aquatic plant rich in cellulose, is often used as a raw material in fuel production. However, natural cellulase can be hardly used in industrial production on account of its low thermal stability and activity. In this study, a metagenomic library was constructed. Then, a new cellulase gene, cel1029, was screened by Congo red staining and expressed in the prokaryotic system. Enzymatic properties of Cel1029 were explored, including optimum temperature and pH, thermal and pH stability, and tolerance against organic solvents, metal ions, and salt solutions. Finally, its ability of degrading water hyacinth was identified and evaluated. Cel1029 displayed high homology with endoglucanase in the glycoside hydrolase family 5 (GH5) and had high stability across a broad temperature range. More than 86% of its enzymatic activities were retained between 4 and 60 degrees C after 24 h of incubation. Single-factor analysis and orthogonal design were further conducted to determine the optimal conditions for the highest reducing sugar yield of water hyacinth. Interestingly, Cel1029 efficiently transformed water hyacinth with a reducing sugar yield of 430.39 mg/g in 22 h. These findings may open the door for significant industrial applications of a novel GH5 cellulase (NCBI Reference Sequence: MK051001, Cel1029) and help identify more efficient methods to degrade cellulose-rich plants. LA - English DB - MTMT ER - TY - JOUR AU - Zhou, Zheng AU - Ju, Xin AU - Chen, Jiajia AU - Wang, Rong AU - Zhong, Yuqing AU - Li, Liangzhi TI - Charge-oriented strategies of tunable substrate affinity based on cellulase and biomass for improving in situ saccharification: A review JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 319 PY - 2021 SN - 0960-8524 DO - 10.1016/j.biortech.2020.124159 UR - https://m2.mtmt.hu/api/publication/31616985 ID - 31616985 N1 - CODEN: BIRTE Correspondence Address: Li, L.; College of Chemistry and Life Science, China; email: liliangzhi01@163.com Funding details: SNG2018046 Funding details: National Natural Science Foundation of China, NSFC, 21676173, 31770903 Funding details: Graduate Research and Innovation Projects of Jiangsu Province, KYCX20_2776 Funding text 1: The authors are grateful for the support from the National Natural Science Foundation of China (Grant No.: 21676173 , Grant No.: 31770903 ), Agricultural Infrastructure Project of Suzhou Science and Technology Development Plan (Grants No.: SNG2018046 ) and Graduate Research and Innovation Projects of Jiangsu Province (CN, Grant No.: KYCX20_2776 ). LA - English DB - MTMT ER - TY - JOUR AU - Brondi, Mariana G. AU - Elias, Andrew M. AU - Furlan, Felipe F. AU - Giordano, Roberto C. AU - Farinas, Cristiane S. TI - Performance targets defined by retro-techno-economic analysis for the use of soybean protein as saccharification additive in an integrated biorefinery JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 10 PY - 2020 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-020-64316-6 UR - https://m2.mtmt.hu/api/publication/31702010 ID - 31702010 AB - The use of additives in the enzymatic saccharification of lignocellulosic biomass can have positive effects, decreasing the unproductive adsorption of cellulases on lignin and reducing the loss of enzyme activity. Soybean protein stands out as a potential lignin-blocking additive, but the economic impact of its use has not previously been investigated. Here, a systematic evaluation was performed of the process conditions, together with a techno-economic analysis, for the use of soybean protein in the saccharification of hydrothermally pretreated sugarcane bagasse in the context of an integrated 1G-2G ethanol biorefinery. Statistical experimental design methodology was firstly applied as a tool to select the process variable solids loading at 15% (w/w) and soybean protein concentration at 12% (w/w), followed by determination of enzyme dosage at 10 FPU/g and hydrolysis time of 24h. The saccharification of sugarcane bagasse under these conditions enabled an increase of 26% in the amount of glucose released, compared to the control without additive. The retro-techno-economic analysis (RTEA) technique showed that to make the biorefinery economically feasible, some performance targets should be reached experimentally such as increasing biomass conversion to ideally 80% and reducing enzyme loading to 5.6 FPU/g in the presence of low-cost soybean protein. LA - English DB - MTMT ER - TY - JOUR AU - Cai, Cheng AU - Bao, Yu AU - Jin, Yu AU - Li, Feiyun AU - Pang, Yuxia AU - Lou, Hongming AU - Qian, Yong AU - Qiu, Xueqing TI - Preparation of high molecular weight pH-responsive lignin-polyethylene glycol (L-PEG) and its application in enzymatic saccharification of lignocelluloses JF - CELLULOSE J2 - CELLULOSE VL - 27 PY - 2020 IS - 2 SP - 755 EP - 767 PG - 13 SN - 0969-0239 DO - 10.1007/s10570-019-02800-7 UR - https://m2.mtmt.hu/api/publication/31448517 ID - 31448517 AB - Highly recyclable pH-responsive lignin-polyethylene glycol (L-PEG) was synthesized to achieve enhanced lignocellulosic hydrolysis and recycling cellulase. The performance of L-PEG could be easily regulated by adjusting the molecular weight and the amount of PEG. The large molecular weight facilitated L-PEG to reduce the invalid adsorption of cellulase on lignin during hydrolysis and enhance its flocculation effect at around pH 3.0. L-PEG(1000-40) obtained by adding 40 wt% (based on lignin) PEG1000 could effectively enhance the enzymatic hydrolysis of lignocelluloses and recover most of cellulase after hydrolysis through simply adjusting the pH of hydrolysate. During eucalyptus hydrolysis, using L-PEG(1000-40) to recycle cellulase could not only save 40% cellulase, but also increase the glucose yield by 121%. Due to the low synthesis cost of L-PEG and the simple and convenient recovery operation, this new method is beneficial to the improvement of lignocellulosic saccharification process and the high-value utilization of lignin. Graphic abstract LA - English DB - MTMT ER - TY - JOUR AU - Cui, Mei AU - Duan, Yuhao AU - Ma, Yuanyuan AU - Al-Shwafy, Khaled W. A. AU - Liu, Yudong AU - Zhao, Xudong AU - Huang, Renliang AU - Qi, Wei AU - He, Zhonghe AU - Su, Rongxin TI - Real-Time QCM-D Monitoring of the Adsorption-Desorption of Expansin on Lignin JF - LANGMUIR J2 - LANGMUIR VL - 36 PY - 2020 IS - 16 SP - 4503 EP - 4510 PG - 8 SN - 0743-7463 DO - 10.1021/acs.langmuir.0c00104 UR - https://m2.mtmt.hu/api/publication/31448515 ID - 31448515 AB - Expansin has nonhydrolytic disruptive activity and synergistically acts with cellulases to enhance the hydrolysis of cellulose. The adsorption-desorption of expansin on noncellulosic lignin can greatly affect the action of expansin on lignocellulose. In this study, three lignins with different sources (kraft lignin (KL), sodium lignin sulfonate (SLS), and enzymatic hydrolysis lignin (EHL)) were selected as the substrates. The real-time adsorption-desorption of Bacillus subtilis expansin (BsEXLX1) on lignins was monitored using quartz crystal microgravimetry with dissipation (QCM-D). The effects of temperature and Tween 80 on the adsorption-desorption behaviors were also investigated. The results show that BsEXLX1 exhibited high binding ability on lignin and achieved maximum adsorption of 283.2, 273.8, and 266.9 ng cm(-2) at 25 degrees C on KL, SLS, and EHL, respectively. The maximum adsorption decreased to 148.2-192.8 ng cm(-2) when the temperature increased from 25 to 45 degrees C. Moreover, Tween 80 competitively bound to lignin and significantly prevented expansin adsorption. After irreversible adsorption of Tween 80, the maximum adsorption of BsEXLX1 greatly decreased to 33.3, 37.2, and 10.3 ng cm(-2) at 25 degrees C on la, SLS, and EHL, respectively. Finally, a kinetic model was developed to analyze the adsorption- desorption process of BsEXLX1. BsEXLX1 has a higher adsorption rate constant (k(A)) and a lower desorption rate constant (k(D)) on KL than on SLS and EHL. The findings of this study provide useful insights into the adsorption-desorption of expansin on lignin. LA - English DB - MTMT ER - TY - JOUR AU - Fu, Jiayin AU - Ding, Xiaochu AU - Stowell, Chelsea E. T. AU - Wu, Yen-Lin AU - Wang, Yadong TI - Slow degrading poly(glycerol sebacate) derivatives improve vascular graft remodeling in a rat carotid artery interposition model JF - BIOMATERIALS J2 - BIOMATERIALS VL - 257 PY - 2020 PG - 14 SN - 0142-9612 DO - 10.1016/j.biomaterials.2020.120251 UR - https://m2.mtmt.hu/api/publication/31702007 ID - 31702007 AB - Porous synthetic grafts made of poly (glycerol sebacate) (PGS) can transform into autologous vascular conduits in vivo upon degradation of PGS. A long-held doctrine in tissue engineering is the necessity to match degradation of the scaffolds to tissue regeneration. Here, we tested the impact of degradation of PGS and its derivative in an interposition model of rat common carotid artery (CCA). Previous work indicates a complete degradation of PGS within approximately 2 weeks, likely at the fast end of the spectrum. Thus, the derivation of PGS focuses on delay degradation by conjugating the free hydroxy groups in PGS with a long chain carboxylic acid: palmitic acid, one of the most common lipid components. We evaluated two of the resultant palmitate-PGS (PPGS) in this study: one containing 9% palmitate (9-PPGS) and the other 16% palmitate (16-PPGS). 16-PPGS grafts had the highest patency. Ultrasound imaging showed that the lumens of 16-PPGS grafts were similar to CCA and smaller than 9-PPGS and PGS grafts 12 weeks post-operation. Immunohistological and histological examination showed an endothelialized lumens in all three types of grafts within 4 weeks. Inflammatory responses to 16-PPGS grafts were limited to the adventitial space in contrast to a more diffusive infiltration in 9-PPGS and PGS grafts in week 4. Examination of calponin(+) and alpha SMA(+) cells revealed that 16-PPGS grafts remodeled into a distinctive bilayered wall, while the walls of 9-PPGS grafts and PGS grafts only had one thick layer of smooth muscle-like cells. Correspondingly, the expression of collagen III and elastin displayed an identical layered structure in the remodeled 16-PPGS grafts, in contrast to a more spread distribution in 9-PPGS and PGS grafts. All the three types of grafts exhibited the same collagen content and burst pressure after 12 weeks of host remodeling. However, the compliance and elastin content of 16-PPGS grafts in week 12 were closest to those of CCA. Overall, placing the degradation of PGS derived elastomer to a window of 4-12 weeks results in vascular conduits closer to arteries in a rat carotid artery interposition model over a 12-week observation period. LA - English DB - MTMT ER - TY - JOUR AU - Haldar, Dibyajyoti AU - Purkait, Mihir Kumar TI - Lignocellulosic conversion into value-added products: A review JF - PROCESS BIOCHEMISTRY J2 - PROCESS BIOCHEM VL - 89 PY - 2020 SP - 110 EP - 133 PG - 24 SN - 1359-5113 DO - 10.1016/j.procbio.2019.10.001 UR - https://m2.mtmt.hu/api/publication/31448520 ID - 31448520 AB - In the present context of energy crisis, exploration of lignocellulosic biomass has emerged as potential substitute to maintain environmental sustainability. However, the conversion of biomass into value-added products still faces challenges to find a suitable unit operation. The stubborn dependency on the cost intensive enzymatic system, limits an effective saccharification to hydrolyze the biomass. India has been one of the top most agriculturally enriched countries with broad scope of utilizing waste residue that remains as an unused biomass at harvested locations. Hence, in the present script, an overview on the latest R&D initiatives taken by Government of India are briefed to highlight the projects based on biofuels and in addition, global scenario of biofuel production is comprehensively discussed. Further, critical analysis on the advancement of different pretreatment operations are highlighted through latest inventions. Thereafter, biochemistry of cellulase enzyme with essential factors were explored to understand the mechanistic interactions involved during saccharification of biomass. An insight on the latest accomplishments of various fermentation process provides an in depth understanding of metabolic engineering based on the genetic studies of fermentative microorganisms. Finally, the article is concluded with brief discussions on fate of the derivatives obtained from macromolecules such as cellulose and lignin. LA - English DB - MTMT ER - TY - JOUR AU - Jampana, Surya R. AU - Jia, Linjing AU - Ramarao, Bandaru V AU - Kumar, Deepak TI - Experimental investigation of the adsorption and desorption of cellulase enzymes on zeolite-beta for enzyme recycling applications JF - BIOPROCESS AND BIOSYSTEMS ENGINEERING J2 - BIOPROC BIOSYST ENG PY - 2020 PG - 11 SN - 1615-7591 DO - 10.1007/s00449-020-02459-6 UR - https://m2.mtmt.hu/api/publication/31700037 ID - 31700037 AB - The recyclability of cellulase enzymes using zeolite and polyethylene glycol (PEG) was investigated. The cellulase enzymes from cellulose hydrolysate suspensions were adsorbed onto zeolite-beta under typical working conditions (pH 5). PEG having a molecular weight of 200 Da and 20 kDa was used as an eluent to desorb the cellulase enzymes from zeolite-beta. Adsorption and desorption profiles of cellulase enzymes were studied by varying pH, PEG concentration, and salt concentration. Maximum binding capacity, q(m)of the zeolite decreased by increasing the pH, or by introducing PEG. At pH 5, the q(m) of the zeolite was determined to be 121 x 10(-3)g/g. About 24%, 51% and 75% of the adsorbed enzyme can be recovered using 1 M NaCl, PEG 200 and PEG 20000, respectively. The specific activity of the recovered enzyme increased by 57% due to the presence of residual PEG. LA - English DB - MTMT ER - TY - JOUR AU - Pallapolu, Venkata R. AU - Shi, Suan AU - Kang, Li AU - Kothari, Urvi AU - Li, Jing TI - Boost Effect of Water-Soluble Polymers on Enzymatic Hydrolysis of Lignocellulosic Biomass JF - INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH J2 - IND ENG CHEM RES VL - 59 PY - 2020 IS - 11 SP - 5186 EP - 5193 PG - 8 SN - 0888-5885 DO - 10.1021/acs.iecr.9b05850 UR - https://m2.mtmt.hu/api/publication/31448522 ID - 31448522 AB - Supplementation of surface-active additives during enzymatic saccharification of biomass helps to increase the activity of cellulase and thus reduces the enzyme dosage in the bioconversion processes. To evaluate the boost effect of additives on enzymatic hydrolysis, five different water-soluble polymers were studied in this work, including poly(vinyl alcohol) (PVA), poly(vinyl pyrrolidone) (PVP), polyethylene glycol) (PEG), cationic polyacrylamide (C-PAM), and polyethyleneimine (PEI). Lignin-containing substrates and lignin-free substrates were used in enzymatic hydrolysis tests. It was found that an addition of 10 mg polymer/g-substrate leads to up to a 25.4% increase in glucan hydrolysis and a 13.2% increase in xylan hydrolysis depending on the type of the substrate and polymer. Glucan digestibility achieved from low enzyme loading of 5 FPU/g-glucan with the polymer is comparable to that from 15 FPU/g-glucan enzyme loading. Reducing the amount of the enzyme required could make biomass conversion more cost-effective. Significantly higher yields were obtained with nonionic polymers (PVP, PVA, and PEG) compared with ionic polymers (PEI and C-PAM) for all substrates. LA - English DB - MTMT ER - TY - JOUR AU - Sun, Dan AU - Yang, Qiaomei AU - Wang, Yanting AU - Gao, Hairong AU - He, Mingxiong AU - Lin, Xinchun AU - Lu, Jun AU - Wang, Youmei AU - Kang, Heng AU - Alam, Aftab AU - Tu, Yuanyuan AU - Xia, Tao AU - Peng, Liangcai TI - Distinct mechanisms of enzymatic saccharification and bioethanol conversion enhancement by three surfactants under steam explosion and mild chemical pretreatments in bioenergy Miscanthus JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 153 PY - 2020 PG - 11 SN - 0926-6690 DO - 10.1016/j.indcrop.2020.112559 UR - https://m2.mtmt.hu/api/publication/31448518 ID - 31448518 AB - Miscanthus is a leading bioenergy crop that represents an enormous lignocellulose resource for biofuels and bioproducts. However, as lignocellulose recalcitrance leads to financially inviable bioethanol production and the potential of secondary wastes into the environment, it becomes crucial to explore green-like and cost-effective biomass processing technologies. To address these issues, low doses of chemical surfactants have been added to enhance biomass enzymatic hydrolysis and bioethanol conversion, but much remains unknown about the mechanism of enhancement. For first time, in this study, a novel chemical surfactant (1% Silwet L-77) was applied to enhance the enzymatic hydrolysis of raw Miscanthus straw, and 40% cellulose digestion was achieved, which is 1.2- and 4.5-fold higher than that of two well-known surfactants (PEG-4000 and Tween-80), respectively. Using lignocellulose substrates obtained from Miscanthus biomass samples that were pretreated by green-like steam explosion followed by mild chemical (NaOH or H2SO4) pretreatments, supplementation with the three surfactants led to significantly enhanced enzymatic saccharification. The 2% Tween-80 supply resulted in a hexose yield of 99% (% cellulose) from enzymatic hydrolysis, followed by 95% with 0.5% PEG-4000 and 71% with 1% Silwet L-77. Despite the slightly lower hexose yield, Silwet L-77 resulted in consistently higher sugar-ethanol conversion rates in all lignocellulose substrates examined. Furthermore, based on the enzyme profiling of mixed cellulase adsorption on lignocellulose and the chemical analysis of wall polymer features and lignocellulose accessibility, this study proposed multiple hypothetical models to interpret the distinct enhancement roles of three surfactants in the enzymatic hydrolyses of diverse lignocellulose substrates. These models also provide a powerful strategy for low-cost bioethanol production with the potential for high-value bioproducts by using desirable surfactants in Miscanthus and other bioenergy crops. LA - English DB - MTMT ER - TY - JOUR AU - Tang, Wei AU - Wu, Xinxing AU - Huang, Chen AU - Huang, Caoxing AU - Lai, Chenhuan AU - Yong, Qiang TI - Humic acid-assisted autohydrolysis of waste wheat straw to sustainably improve enzymatic hydrolysis JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 306 PY - 2020 PG - 8 SN - 0960-8524 DO - 10.1016/j.biortech.2020.123103 UR - https://m2.mtmt.hu/api/publication/31448525 ID - 31448525 AB - Humic acid (HA), considered the main component of organic matter in the ash of waste wheat straw (WWS), has the potential to improve autohydrolysis through its function as a surfactant. In this work, a pre-washed WWS (PWWS) was subjected to autohydrolysis with addition of HA to explore whether its surfactant properties can provide benefit to biorefinery operations. Acquired results showed that HA acted as delignification agent likely due to its surfactant properties. Delignification was more than doubled at the maximum HA dosage (30 g/L) relative to the control, which allowed for enzymatic hydrolysis efficiency to also increase from 64.9% to 81.8%. The pretreated materials were further subjected to analysis structure characterization. The results showed that HA effectively reduced the surface lignin area of PWWS, lowering non-specific adsorption of lignin to enzymes. The autohydrolysis with HA was an effective technique to improve the subsequent cellulose enzymatic digestion by enhancing the delignification. LA - English DB - MTMT ER - TY - JOUR AU - Wang, Jinye AU - Wang, Jia AU - Lu, Zhoumin AU - Zhang, Junhua TI - Adsorption and desorption of cellulase on/from enzymatic residual lignin after alkali pretreatment JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 155 PY - 2020 PG - 6 SN - 0926-6690 DO - 10.1016/j.indcrop.2020.112811 UR - https://m2.mtmt.hu/api/publication/31702009 ID - 31702009 AB - The knowledge about adsorption/desorption of cellulase on/from residual lignin is essential for efficient cellulose hydrolysis by recycling the cellulase from residual solid fraction. Herein, the impact of alkali pretreatment at different alkali strength on the adsorption/desorption of enzymatic residual lignins (ERLs) with cellulase was investigated. ERLs pretreated at stronger alkali strength had higher molecular weights and negative zeta potentials, but lower hydrophobicity. The inhibition of ERL to the enzymatic cellulose digestion was mitigated by alkali pretreatment, and inhibition degrees decreased from 21.5 % (with un-pretreated ERL) to 18.9 %, 12.9 %, and 10.8 % with the addition of ERLs treated with 0.5 %, 1.0 %, and 1.5 % (w/w) sodium hydroxide aqueous solution, respectively. The affinity of ERLs with cellulase diminished after alkali pretreatment, and the binding strength decreased from 183.0 to 130.7 mL/g with the alkali strength increasing, retaining more cellulase activities in supernatant. Desorption capacity of bound-cellulase from ERL increased after alkali pretreatment, and the recovery increased from 65.3% to 73.1% with the alkali strength increasing. The cellulase released after desorption exhibited hydrolytic activity, and higher glucose yields were obtained by the bound-cellulase on alkali-pretreated ERLs compared with that of un-pretreated ERL. The results provide references for the development of alkali pretreatment and recycling cellulase from residual lignin for efficient lignocellulosics digestion. LA - English DB - MTMT ER - TY - JOUR AU - Zheng, Tianran AU - Yu, Hailong AU - Liu, Shijie AU - Jiang, Jianxin AU - Wang, Kun TI - Achieving high ethanol yield by co-feeding corncob residues and tea seed cake at high-solids simultaneous saccharification and fermentation JF - RENEWABLE ENERGY J2 - RENEW ENERGY VL - 145 PY - 2020 SP - 858 EP - 866 PG - 9 SN - 0960-1481 DO - 10.1016/j.renene.2019.06.083 UR - https://m2.mtmt.hu/api/publication/31059623 ID - 31059623 N1 - Funding Agency and Grant Number: Guangxi Key Laboratory of Chemistry and Engineering of Forest Products [GXFC16-01]; specific research project of Guangxi for research bases and talents [AD18126005]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2017PT13]; China Scholarship CouncilChina Scholarship Council Funding text: The authors are grateful for the Guangxi Key Laboratory of Chemistry and Engineering of Forest Products (GXFC16-01), the specific research project of Guangxi for research bases and talents (AD18126005), and the Fundamental Research Funds for the Central Universities (2017PT13). We also appreciate support from the China Scholarship Council. AB - The feasibility of high-solids simultaneous saccharification and ethanol production was investigated using unwashed corncob residues (CRs) with tea-seed cake (TSC). Tea saponin of TSC could serve as an accelerant to enhance ethanol production and reduce the dosage of enzymes. The proteins of TSC could be the nitrogen resource for yeast. The effects of substrate concentration (10%, 15%, and 20% (w/w)), enzyme dosage (2.5-15 FPU/g-cellulose), and types of media on ethanol production were evaluated. An ethanol yield of 86.56% of the theoretical maximum could be obtained at a substrate concentration of 15% (w/w) with 10 FPUJg-cellulose. Furthermore, fermentations in different media showed that the surface tension (49.21 mN/m) and contact angle (42.6 degrees) of the fermentation system with TSC were lower than those from the other systems. This study found that adding TSC to the fermentation system was an attractive strategy to achieve high ethanol yield without any pretreatment. Comprehensive utilization of CRs and TSC as feedstocks for ethanol production can reduce the cost of biorefineries with environmental benefits. (C) 2019 Elsevier Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Brondi, Mariana G. AU - Vasconcellos, Vanessa M. AU - Giordano, Roberto C. AU - Farinas, Cristiane S. TI - Alternative Low-Cost Additives to Improve the Saccharification of Lignocellulosic Biomass JF - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY J2 - APPL BIOCHEM BIOTECH VL - 187 PY - 2019 IS - 2 SP - 461 EP - 473 PG - 13 SN - 0273-2289 DO - 10.1007/s12010-018-2834-z UR - https://m2.mtmt.hu/api/publication/30565451 ID - 30565451 AB - A potential strategy to mitigate problems related to unproductive adsorption of enzymes onto lignin during the saccharification of lignocellulosic biomass is the addition of lignin-blocking agents to the hydrolysis reaction medium. However, there is a clear need to find more cost-effective additives for use in large-scale processes. Here, selected alternative low-cost additives were evaluated in the saccharification of steam-exploded sugarcane bagasse using a commercial enzymatic cocktail. The addition of soybean protein, tryptone, peptone, and maize zein had positive effects on glucose release during the hydrolysis, with gains of up to 36% when 8% (w/w) soybean protein was used. These improvements were superior to those obtained using bovine serum albumin (BSA), a much more expensive protein that has been widely reported for such an application. Moreover, addition of soybean protein led to a saving of 48h in the hydrolysis, corresponding to a 66% decrease in the reactor operation time required. In order to achieve the same hydrolysis yield without the soybean additive, the enzyme loading would need to be increased by 50%. FTIR spectroscopy and nitrogen elemental analysis revealed that the additives probably acted to reduce unproductive binding of cellulolytic enzymes onto the lignin portion of the sugarcane bagasse. LA - English DB - MTMT ER - TY - JOUR AU - Li, Huanan AU - Wang, Chaoying AU - Xiao, Wenjing AU - Yang, Yuxian AU - Hu, Pan AU - Dai, Yujun AU - Jiang, Zhengbing TI - Dissecting the effect of polyethylene glycol on the enzymatic hydrolysis of diverse lignocellulose JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 131 PY - 2019 SP - 676 EP - 681 PG - 6 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2019.03.131 UR - https://m2.mtmt.hu/api/publication/31021093 ID - 31021093 AB - Natural lignocellulose is used as raw material to produce chemicals through biological transformation. The accessibility of cellulase to substrate was also one of the limiting factors of industrial production. Polyethylene glycol (PEG) can be used as additive in enzymatic hydrolysis of lignocellulose. In this study, enzymatic activity on simultaneous or non-simultaneous addition of PEG 4000 was investigated, and the partly delignified rice straw, the rice straw and filter paper were used as substrates, respectively. Enzyme activity was characterized by reducing sugar concentration in supernatant which was quantified through 3,5-dinitrosalicylic acid (DNS) method. Addition of PEG has been proven to facilitate enzymatic hydrolysis of lignocellulosic materials. Furthermore, PEG had the positive effect on hydrolytic enzyme activity of pure cellulose materials without lignin. Changes in lignocellulose materials have been observed by inverted microscope and Scanning electron microscope (SEM), and no chemical changes were shown by Fourier transform infrared spectroscopy (FTIR). The promotion of PEG on enzymatic hydrolysis of pure cellulose materials may be due to its loose physical structure and similar phenomenon in natural lignin materials. PEG loosens the physical structure of lignocellulose, thus facilitating enzymatic hydrolysis. This may be a new idea to optimize the lignocellulosic enzymatic hydrolysis process. (C) 2019 Elsevier B.V. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Li, Mengxing AU - Eskridge, Kent AU - Liu, Enshi AU - Wilkins, Mark TI - Enhancement of polyhydroxybutyrate (PHB) production by 10-fold from alkaline pretreatment liquor with an oxidative enzyme-mediator-surfactant system under Plackett-Burman and central composite designs JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 281 PY - 2019 SP - 99 EP - 106 PG - 8 SN - 0960-8524 DO - 10.1016/j.biortech.2019.02.045 UR - https://m2.mtmt.hu/api/publication/31059627 ID - 31059627 AB - In this study, Plackett-Burman and central composite designs were applied to improve polyhydroxybutyrate (PHB) production from alkaline pretreatment liquor (APL) by Cupriavidus necator DSM 545 using a supplement system consisting of oxidative enzymes (laccase, aryl alcohol oxidase (AAO)), mediators (ABTS, HOBT), DMSO, silica nanoparticle Aerosol R816 and surfactant Tween 80. First, screening experiments under Plackett-Burman design showed R816, ABTS and Tween 80 could significantly enhance PHB production. Additional experiments showed that HOBT and DMSO could be removed, and laccase and AAO were needed to remain in the system. Second, a central composite design was applied to obtain the optimum supplemental levels of R816, ABTS and Tween 80. Under optimum conditions, theoretical maximum PHB production (1.9 g/L) was close to experimental PHB production (2.1 g/L). With the supplement system, a 10-fold increase was achieved compared to PHB production (0.2 g/L) without any supplements. LA - English DB - MTMT ER - TY - JOUR AU - Lin, Xuliang AU - Yang, Yaosen AU - Wu, Lei AU - Wu, Linjun AU - Xu, Danyuan AU - Qin, Yanlin TI - Improved enzymatic hydrolysis of hardwood and cellulase stability by biomass kraft lignin-based polyoxyethylene ether JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 136 PY - 2019 SP - 540 EP - 546 PG - 7 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2019.06.105 UR - https://m2.mtmt.hu/api/publication/31059624 ID - 31059624 AB - Water-soluble kraft lignin-based polyoxyethylene ether (KL-PEG), synthesized from the black liquor of kraft pulping and PEG, was used to improve the enzymatic hydrolysis efficiency of dilute acid pretreated (DA pretreated) Eucalyptus hardwood and cellulase stability. The physicochemical properties of KL-PEG polymer such as solubility, surface tension, charge and aggregation behavior in the solution were first studied. KL-PEG could enhance the enzymatic hydrolysis of Avicel and DA-pretreated Eucalyptus from 63.6% and 58.3% to 78.5% and 93.8%, respectively. The enzymatic activity of cellulase after the enzymatic hydrolysis of Avicel and DA-pretreated Eucalyptus for 72 h remained approximately 84% and 44% in the presence of KL-PEG polymer. KL-PEG could improve the stability and longevity of the cellulase, facilitate the recovery and save the amount of cellulase. The efficient utilization of the pulping black liquor lignin was of great significance to alleviate the pressure brought by the shortage of petrochemical resources, and build an energy-saving and low-carbon society. (C) 2019 Elsevier B.V. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Liu, Shanshan AU - He, Huili AU - Fu, Xin AU - Wang, Yingchao AU - Wang, Qiang AU - Yang, Guihua AU - Chen, Jiachuan AU - Ni, Yonghao TI - Tween 80 enhancing cellulasic activation of hardwood kraft-based dissolving pulp JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 137 PY - 2019 SP - 144 EP - 148 PG - 5 SN - 0926-6690 DO - 10.1016/j.indcrop.2019.05.026 UR - https://m2.mtmt.hu/api/publication/31059619 ID - 31059619 AB - Cellulase treatment is a promising approach to activate dissolving pulp in terms of viscosity and reactivity. However, the enzyme cost is still too high, which hinders the scale up application. In this study, Tween 80 was introduced into the enzymatic system for improving the cellulase efficiency by improving the enzyme accessibility and its stability. Results show that the cellulose chain scission rate was more than 2 times higher with the addition of Tween 80 (0.1 g/L) in comparison to the control, which is mainly attributed to decreased surface tension (by 28.0% with the addition of 0.1 g/L Tween) and increased relative cellulase activity (by 9% at 48 h and 55 degrees C). As a result, Tween 80 assisted cellulase activation gave a higher cellulasic efficiency in terms of viscosity decrease and Fock reactivity increase. Other pulp properties (alkali solubility, molecular weight distribution) and pulp yield were all favorable, supporting the conclusion that the Tween 80- assisted cellulase treatment process has a great potential to improve the cellulase treatment performance of dissolving pulp. LA - English DB - MTMT ER - TY - JOUR AU - McIntosh, S. AU - Palmer, J. AU - Egbuta, M. AU - Liu, L. AU - Vancov, Tony TI - Improved Cellulosic Ethanol Titres from Highly Lignified Cotton Trash Residues Using Various Batch and Fed-Batch Process Configurations JF - BIOENERGY RESEARCH J2 - BIOENERG RES VL - 12 PY - 2019 IS - 4 SP - 1021 EP - 1032 PG - 12 SN - 1939-1234 DO - 10.1007/s12155-019-10023-8 UR - https://m2.mtmt.hu/api/publication/31058043 ID - 31058043 AB - This study investigates a fed-batch simultaneous saccharification fermentation (F-SSF) process to increase ethanol titres from highly lignified (41.6 wt.%) cotton gin trash residue. The optimal initial solid loading, enzyme dose, feed quantities and intervals to maximize substrate feed and subsequent ethanol titres were examined. Under batch SSF conditions, initial extracted cotton gin trash (ECGT) solid loadings were maximised at 19.35 wt.% and attained an ethanol titre of 23.3 g/l with a corresponding yield of 53.7%. Operating under optimised F-SSF mode, fermentations were initiated with 16.13 wt% EGCT solids followed by fresh ECGT feeds of 16.13 wt% and 12.9 wt.% at 12-h intervals. Cellulase levels were maintained at 44 FPU/g glucan throughout the fermentations. The final ethanol titre of 41 .4 g/l with a corresponding conversion rate of 70.1% was achieved after 72 h. Comparable ethanol yields of 40 g/l with 67.8% conversion were realized with lower cellulase dosing (25 FPU g/glucan) but only after extending the fermentation by 24 h. LA - English DB - MTMT ER - TY - JOUR AU - Nitsos, Christos K. AU - Lazaridis, Polykarpos A. AU - Mach-Aigner, Astrid AU - Matis, Kostas A. AU - Triantafyllidis, Konstantinos S. TI - Enhancing Lignocellulosic Biomass Hydrolysis by Hydrothermal Pretreatment, Extraction of Surface Lignin, Wet Milling and Production of Cellulolytic Enzymes JF - CHEMSUSCHEM J2 - CHEMSUSCHEM VL - 12 PY - 2019 IS - 6 SP - 1179 EP - 1195 PG - 17 SN - 1864-5631 DO - 10.1002/cssc.201802597 UR - https://m2.mtmt.hu/api/publication/31059630 ID - 31059630 AB - Acetone and ethanol extraction of lignin deposits from the surface of hydrothermally (liquid hot water) pretreated beech wood biomass alleviates the lignin inhibitory effects during enzymatic hydrolysis of cellulose to glucose and boosts the enzymatic digestibility to high values (approximate to 70 %). Characterization of the extracted lignins (FTIR, pyrolysis/GC-MS, differential thermogravimetry, gel permeation chromatography) indicated high purity, low molecular weight, and features that suggest that it consists mainly of fragments of the native wood lignin partially depolymerized and recondensed on the biomass surface during the hydrothermal pretreatment. The pyrolysis products of the extracted surface lignins suggest their high potential as a feedstock for the production of high added value phenolic compounds. When the enzymatic hydrolysis of the pretreated and extracted biomass solids was assisted by mild wet milling, near complete cellulose digestibility (>= 95 %) could be achieved. In the context of the biorefinery and whole-biomass valorization concept, it was also shown that the hydrothermally (hemicellulose-deficient) pretreated and delignified biomass solids could be also successfully used for the production of crude cellulase from Trichoderma reesei cultures, providing a simple and low-cost method for the complementary production of cellulases by utilizing fractions of the integrated hydrolysis process. LA - English DB - MTMT ER - TY - JOUR AU - Ren, Gaihuan AU - Sun, Zhicheng AU - Wang, Zengzi AU - Zheng, Xiaoyang AU - Xu, Zhenghe AU - Sun, Dejun TI - Nanoemulsion formation by the phase inversion temperature method using polyoxypropylene surfactants JF - JOURNAL OF COLLOID AND INTERFACE SCIENCE J2 - J COLLOID INTERF SCI VL - 540 PY - 2019 SP - 177 EP - 184 PG - 8 SN - 0021-9797 DO - 10.1016/j.jcis.2019.01.018 UR - https://m2.mtmt.hu/api/publication/31059633 ID - 31059633 AB - Hypothesis: Surfactants with temperature-sensitive polyoxyethylene (POE) chains are widely used to prepare nanoemulsions by the phase inversion temperature (PIT) method. It is therefore anticipated that surfactants with temperature-sensitive polyoxypropylene (POP) chains could also be used to prepare nanoemulsions by the PIT method.Experiment: POP surfactants were synthesized through electrostatic interactions between hydrophilic POP diamines and hydrophobic long-chain fatty acids. The synthesized POP surfactants were used as emulsifiers to prepare n-tetradecane-in-water nanoemulsions by the PIT method. Electrical conductivity measurements were used to determine the PITs of the water/POP surfactant/n-tetradecane systems. The effects of surfactant concentration, NaCI concentration, number of POP units, and degree of unsaturation of hydrocarbon chains on the PIT and the nanoemulsion droplet size were investigated. The droplet size and morphology of the nanoemulsions were characterized by dynamic light scattering and cryogenic transmission electron microscopy, respectively.Findings: Nanoemulsions are formed by the PIT method using POP surfactants when the POP chains are short (similar to 2.5-6.1 POP units). The formation of nanoemulsions with droplet radii of 20-300 nm and spherical morphology occurs because of the temperature-dependent hydration of the short POP chains. (C) 2019 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Zhou, Yawen AU - Yang, Jian AU - Luo, Cong AU - Yang, Bo AU - Liu, Changyao AU - Xu, Baocai TI - Effect of Metal Ions and Surfactants on the Enzymatic Hydrolysis of Pretreated Lignocellulose JF - BIORESOURCES J2 - BIORESOURCES VL - 14 PY - 2019 IS - 1 SP - 1653 EP - 1667 PG - 15 SN - 1930-2126 DO - 10.15376/biores.14.1.1653-1667 UR - https://m2.mtmt.hu/api/publication/30565450 ID - 30565450 AB - The effect of metal ions and surfactants on the enzymatic hydrolysis of pretreated wheat straw lignocellulose was investigated. Scanning electron microscopy, infrared spectrum analysis, dynamic light scattering, and fluorescence spectra analysis were used to characterize the influence of Fe3+/ polyoxyethylene (20) sorbitan monooleate (Tween 80). The interaction between Fe3+/Tween 80 and enzyme was further investigated by enzyme kinetics and enzyme activity measurements. The best synergistic effect was obtained when the ratio of Fe3+ and Tween 80 was 0.06. The scanning electron microscopy images showed that the Fe3+/Tween 80 combination was associated with high porosity substrates. The infrared spectrum analysis indicated that the components of the substrates depended on additive types. The highest relative enzymatic activity increase rate was obtained with added Fe3+/ Tween 80. The V-max and K-m values of the group with added Fe3+/Tween 80 were much higher than that of the group without additives. With the addition of Fe3+/ Tween 80, the intensity of the fluorescence emission peak decreased and the peak shifted towards a longer wavelength. LA - English DB - MTMT ER - TY - JOUR AU - Alhammad, A AU - Adewale, P AU - Kuttiraja, M AU - Christopher, L P TI - Enhancing enzyme-aided production of fermentable sugars from poplar pulp in the presence of non-ionic surfactants JF - BIOPROCESS AND BIOSYSTEMS ENGINEERING J2 - BIOPROC BIOSYST ENG VL - 41 PY - 2018 IS - 8 SP - 1133 EP - 1142 PG - 10 SN - 1615-7591 DO - 10.1007/s00449-018-1942-z UR - https://m2.mtmt.hu/api/publication/27579933 ID - 27579933 LA - English DB - MTMT ER - TY - JOUR AU - Djajadi, Demi T AU - Jensen, Mads M AU - Oliveira, Marlene AU - Jensen, Anders AU - Thygesen, Lisbeth G AU - Pinelo, Manuel AU - Glasius, Marianne AU - Jorgensen, Henning AU - Meyer, Anne S TI - Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 11 PY - 2018 PG - 13 SN - 1754-6834 DO - 10.1186/s13068-018-1085-0 UR - https://m2.mtmt.hu/api/publication/27325611 ID - 27325611 LA - English DB - MTMT ER - TY - JOUR AU - Djajadi, Demi T. AU - Pihlajaniemi, Ville AU - Rahikainen, Jenni AU - Kruus, Kristiina AU - Meyer, Anne S. TI - Cellulases adsorb reversibly on biomass lignin JF - BIOTECHNOLOGY AND BIOENGINEERING J2 - BIOTECHNOL BIOENG VL - 115 PY - 2018 IS - 12 SP - 2869 EP - 2880 PG - 12 SN - 0006-3592 DO - 10.1002/bit.26820 UR - https://m2.mtmt.hu/api/publication/30565454 ID - 30565454 AB - Adsorption of cellulases onto lignin is considered a major factor in retarding enzymatic cellulose degradation of lignocellulosic biomass. However, the adsorption mechanisms and kinetics are not well understood for individual types of cellulases. This study examines the binding affinity, kinetics of adsorption, and competition of four monocomponent cellulases of Trichoderma reesei during adsorption onto lignin. TrCel7A, TrCel6A, TrCel7B, and TrCel5A were radiolabeled for adsorption experiments on lignin-rich residues (LRRs) isolated from hydrothermally pretreated spruce (L-HPS) and wheat straw (L-HPWS), respectively. On the basis of adsorption isotherms fitted to the Langmuir model, the ranking of binding affinities was TrCel5A > TrCel6A > TrCel7B > TrCel7A on both types of LRRs. The enzymes had a higher affinity to the L-HPS than to the L-HPWS. Adsorption experiments with dilution after 1 and 24 hr and kinetic modeling were performed to quantify any irreversible binding over time. Models with reversible binding parameters fitted well and can explain the results obtained. The adsorption constants obtained from the reversible models agreed with the fitted Langmuir isotherms and suggested that reversible adsorption-desorption existed at equilibrium. Competitive binding experiments showed that individual types of cellulases competed for binding sites on the lignin and the adsorption data fitted the Langmuir adsorption model. Overall, the data strongly indicate that the adsorption of cellulases onto lignin is reversible and the findings have implications for the development of more efficient cellulose degrading enzymes. LA - English DB - MTMT ER - TY - JOUR AU - Farinas, Cristiane S AU - Marconcini, Jose Manoel AU - Mattoso, Luiz Henrique C TI - Enzymatic Conversion of Sugarcane Lignocellulosic Biomass as a Platform for the Production of Ethanol, Enzymes and Nanocellulose JF - JOURNAL OF RENEWABLE MATERIALS J2 - J RENEW MATERIALS VL - 6 PY - 2018 IS - 2 SP - 203 EP - 216 PG - 14 SN - 2164-6325 DO - 10.7569/JRM.2017.6341578 UR - https://m2.mtmt.hu/api/publication/27325612 ID - 27325612 N1 - Cited By :1 Export Date: 30 January 2019 Correspondence Address: Farinas, C.S.; Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentation, Rua XV de Novembro 1452, Brazil; email: cristiane.farinas@embrapa.br Funding details: Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, 401182/2014-2 Funding details: Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, 2014/19000-3 Funding details: Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, 2016/10636-8 Funding text 1: The author would like to thank Embrapa, CNPq (Process 401182/2014-2), SISNANO/MCTIC, Capes, and FAPESP (Processes 2014/19000-3 and 2016/10636-8) (all from Brazil) for financial support, and the students and technicians from Embrapa Instrumentation for their contribution. LA - English DB - MTMT ER - TY - JOUR AU - Fatriasari, Widya AU - Adi, D T Nugroho AU - Laksana, R P B AU - Fajriutami, T AU - Raniya, R AU - Ghozali, M AU - Hermiati, E TI - The effect of amphipilic lignin derivatives addition on enzymatic hydrolysis performance of kraft pulp from sorghum bagasse JF - IOP CONFERENCE SERIES: EARTH AND ENVIRONMENTAL SCIENCE J2 - IOP CONF SER EARTH AND ENVIRON SCI VL - 141 PY - 2018 IS - Bogor PG - 7 SN - 1755-1307 DO - 10.1088/1755-1315/141/1/012005 UR - https://m2.mtmt.hu/api/publication/27579936 ID - 27579936 LA - English DB - MTMT ER - TY - JOUR AU - Herbaut, Mickael AU - Zoghlami, Aya AU - Paes, Gabriel TI - Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 11 PY - 2018 PG - 13 SN - 1754-6834 DO - 10.1186/s13068-018-1267-9 UR - https://m2.mtmt.hu/api/publication/30565455 ID - 30565455 AB - Background: Improving lignocellulolytic enzymes' diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification. Although many chemical features are considered as detrimental to saccharification, enzymes' dynamics within the cell walls remains poorly explored and understood. To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose. In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification. LA - English DB - MTMT ER - TY - JOUR AU - Jain, Lavika AU - Kurmi, Akhilesh Kumar AU - Agrawal, Deepti TI - Feasibility studies with lignin blocking additives in enhancing saccharification and cellulase recovery: Mutant UV-8 of T. verruculosus IIPC 324 a case study JF - ENZYME AND MICROBIAL TECHNOLOGY J2 - ENZYME MICROB TECH VL - 118 PY - 2018 SP - 44 EP - 49 PG - 6 SN - 0141-0229 DO - 10.1016/j.enzmictec.2018.07.003 UR - https://m2.mtmt.hu/api/publication/30565432 ID - 30565432 AB - The process economics of fermentable sugar production is dependent on the performance of cellulase cocktail on realistic lignocellulosic biomass and their capability to be recovered and recycled. Feasibility studies were conducted to enhance the digestibility of acid pretreated sugarcane bagasse using novel cellulase cocktail obtained from stable mutant UV-8 of Talaromyces verruculosus IIPC 324 in presence of lignin blocking additives. PEG 6000 was shortlisted as the best additive as it could simultaneously enhance saccharification and overall cellulase recoveries namely cellobiohydrolase, endoglucanase and cellobiase. Addition of 0.3 g PEG 6000/g acidinsoluble lignin content, resulted in 55% and 49.2% saccharification yields in terms of reducing sugars and glucose respectively using this cellulase cocktail (25 mg protein/g cellulose content) after 72 h from acid pre-treated sugarcane bagasse loaded at 7.5%. The study also suggested that the endoglucanase of this mutant was unique with high desorption capability as 85% activity was observed in the saccharified broth devoid of any lignin blocking additive. At its optimum concentration, PEG 6000 was able to retain 94 +/- 0.79% cellobiohydrolase I and 97.97 +/- 1.16% cellobiase enzyme in the saccharified broth which were otherwise lost in residual biomass by similar to 80%, in the absence of this polymeric additive. These results suggest that PEG 6000 was the most promising facilitator for recycling of cellulases obtained from mutant UV-8 of Talaromyces verruculosus IIPC 324 in particular. It paved a way towards the production of cheaper fermentable sugars which serve as a starting raw material for the production of green chemicals and fuels. LA - English DB - MTMT ER - TY - JOUR AU - Kadhum, Haider Jawad AU - Rajendran, Karthik AU - Murthy, Ganti S. TI - Optimization of Surfactant Addition in Cellulosic Ethanol Process Using Integrated Techno-economic and Life Cycle Assessment for Bioprocess Design JF - ACS SUSTAINABLE CHEMISTRY & ENGINEERING J2 - ACS SUSTAIN CHEM ENG VL - 6 PY - 2018 IS - 11 SP - 13687 EP - 13695 PG - 17 SN - 2168-0485 DO - 10.1021/acssuschemeng.8b00387 UR - https://m2.mtmt.hu/api/publication/30565453 ID - 30565453 AB - Surfactants have been demonstrated to be effective in increasing the cellulase enzyme efficacy and overall enzymatic hydrolysis efficiency. However, the impact of the surfactant addition on the economic viability and environmental impacts of the bioethanol process has not been well-investigated. The objective of this study was to determine the economic and the environmental impacts of using five surfactant types-polyethylene glycol (PEG) 3000, PEG4000, PEG6000, PEG8000, and Tween80-at various concentrations (8%, 5%, 2%, 1%, 0.75%, 0.5%, 0.25%, and 0% (w/w)) during enzymatic hydrolysis and fermentation of pretreated Banagrass. We used an integrated techno-economic and life cycle assesment to guide the selection of optimal surfactant concentration in the bioethanol process. A surfactant concentration of >2% negatively affects the profitability of ethanol, even when there is a statistically significant increase in glucose and ethanol titers. Based on the overall performance indicators for final ethanol, economic viability and environmental impacts, the addition of PEG6000 at 2% (w/w) were determined to be the optimal option. Glucose and ethanol concentrations of 119.2 +/- 5.4 g/L and 55.0 +/- 5.8 g/L, respectively, with an 81.5% cellulose conversion rate, were observed for 2% (w/w) PEG6000. Techno-economic and life cycle analysis indicated that 2% w/w PEG6000 addition resulted in ROI of 3.29% and had reduced the global warming potential by 6 g CO2/MJ ethanol produced. LA - English DB - MTMT ER - TY - JOUR AU - Kamsani, Noratiqah AU - Salleh, Madihah Md AU - Basri, Siti Aisyah AU - Mohamad, Shaza Eva AU - Abd, Aziz Suraini AU - Kamaruddin, Kamarulzaman TI - Effects of Surfactant on the Enzymatic Degradation of Oil Palm Empty Fruit Bunch (OPEFB) JF - WASTE AND BIOMASS VALORIZATION J2 - WASTE BIOMASS VALORI VL - 9 PY - 2018 IS - 5 SP - 845 EP - 852 PG - 8 SN - 1877-2641 DO - 10.1007/s12649-017-9838-8 UR - https://m2.mtmt.hu/api/publication/27579935 ID - 27579935 LA - English DB - MTMT ER - TY - JOUR AU - Kushwah, Varun AU - Katiyar, Sameer S. AU - Agrawal, Ashish Kumar AU - Saraf, Isha AU - Singh, Inder Pal AU - Lamprou, Dimitrios A. AU - Gupta, Ramesh C. AU - Jain, Sanyog TI - Implication of linker length on cell cytotoxicity, pharmacokinetic and toxicity profile of gemcitabine-docetaxel combinatorial dual drug conjugate JF - INTERNATIONAL JOURNAL OF PHARMACEUTICS J2 - INT J PHARM VL - 548 PY - 2018 IS - 1 SP - 357 EP - 374 PG - 18 SN - 0378-5173 DO - 10.1016/j.ijpharm.2018.07.016 UR - https://m2.mtmt.hu/api/publication/30565458 ID - 30565458 AB - The present study investigates effect of linkers [zero length (without linker), short length linker (glycine and lysine) and long length linker (PEG1000, PEG2000 and PEG3500)] on pharmacokinetics and toxicity of docetaxel (DTX) and gemcitabine (GEM) bio-conjugates. Conjugates were synthesized via carbodiimide chemistry and characterized by H-1 NMR and FTIR. Conjugation of DTX and GEM via linkers showed diverse physio-chemical and plasma stability profile. Cellular uptake mechanism in MCF-7 and MDA-MB-231 cell lines revealed clathrin mediated internalization of bio-conjugates developed by using long length linkers, leading to higher cytotoxicity compared with free drug congeners. DTX-PEG3500-GEM and DTX-PEG2000-GEM demonstrated 4.21 and 3.81-fold higher AUC((0-infinity)) of GEM in comparison with GEM alone. DTX-PEG2000-GEM and DTX-PEG3500-GEM exhibited reduced hepato-, nephro- and haemolytic toxicity as evident via histopathology, biochemical markers and SEM analysis of RBCs. Conclusively, PEG2000 and PEG3500 significantly improved pharmacokinetics without any sign of toxicity and hence can be explored further for the development of dual-drug conjugates for better therapeutic efficacy. LA - English DB - MTMT ER - TY - JOUR AU - Lou, Hongming AU - Lin, Meilu AU - Zeng, Meijun AU - Cai, Cheng AU - Pang, Yuxia AU - Yang, Dongjie AU - Qiu, Xueqing TI - Effect of Urea on the Enzymatic Hydrolysis of Lignocellulosic Substrate and Its Mechanism JF - BIOENERGY RESEARCH J2 - BIOENERG RES VL - 11 PY - 2018 IS - 2 SP - 456 EP - 465 PG - 10 SN - 1939-1234 DO - 10.1007/s12155-018-9910-7 UR - https://m2.mtmt.hu/api/publication/27579934 ID - 27579934 LA - English DB - MTMT ER - TY - JOUR AU - Lou, Hongming AU - Zeng, Meijun AU - Hu, Qiaoyan AU - Cai, Cheng AU - Lin, Xuliang AU - Qiu, Xueqing AU - Yang, Dongjie AU - Pang, Yuxia TI - Nonionic surfactants enhanced enzymatic hydrolysis of cellulose by reducing cellulase deactivation caused by shear force and air-liquid interface JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 249 PY - 2018 SP - 1 EP - 8 PG - 8 SN - 0960-8524 DO - 10.1016/j.biortech.2017.07.066 UR - https://m2.mtmt.hu/api/publication/27325613 ID - 27325613 N1 - Megjegyzés-27119800 N1 Funding details: 2013B051000011 N1 Funding details: 2016TX03Z298, Guangzhou Science and Technology Program key projects N1 Funding details: 201707020025, Guangzhou Science and Technology Program key projects N1 Funding details: 21376100, NSFC, National Natural Science Foundation of China N1 Funding details: 21676109, NSFC, National Natural Science Foundation of China School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China Cited By :7 Export Date: 30 January 2019 CODEN: BIRTE Correspondence Address: Pang, Y.; School of Chemistry and Chemical Engineering, South China University of TechnologyChina; email: cehyxpang@scut.edu.cn Chemicals/CAS: cellulase, 9012-54-8; microcrystalline cellulose, 39394-43-9, 51395-75-6; cellulose, 61991-22-8, 68073-05-2, 9004-34-6; Cellulase; Cellulose; Surface-Active Agents Funding details: Medical Science and Technology Foundation of Guangdong Province, 2013B051000011 Funding details: 2016TX03Z298 Funding details: Guangzhou Science and Technology Program key projects, 201707020025 Funding details: National Natural Science Foundation of China, NSFC, 21376100 Funding details: National Natural Science Foundation of China, NSFC, 21676109 Funding text 1: The authors acknowledge the financial supports of the National Natural Science Foundation of China ( 21676109 , 21376100 ), Science and Technology Program of Guangzhou ( 201707020025 ), Guangdong Special Support Plan (2016TX03Z298) and Guangdong Province Science and Technology Plan (2013B051000011). Appendix A LA - English DB - MTMT ER - TY - JOUR AU - Lu, Xianqin AU - Feng, Xiaoting AU - Li, Xuezhi AU - Zhao, Jian TI - The adsorption properties of endoglucanase to lignin and their impact on hydrolysis JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 267 PY - 2018 SP - 110 EP - 116 PG - 7 SN - 0960-8524 DO - 10.1016/j.biortech.2018.06.031 UR - https://m2.mtmt.hu/api/publication/30565456 ID - 30565456 AB - Nonproductive adsorption of cellulase to lignin dramatically influenced the hydrolysis efficiency of lignocellulose. By comparing the adsorption behaviors of CBH and EG, we found that the adsorption of EG to lignin showed lower adsorption velocity and capacity versus CBH. During the adsorption of EG to lignin, carbohydrate binding domain (CBM) and catalytic domain (CD) both played an important role by a two-step adsorption process, in which CD slowly bond on lignin and developed stronger interaction with lignin. The optimal binding position of EG on lignin was consistent with that on polysaccharide located in the open catalytic tunnel. So, the adsorption of EG to lignin not only limited the movement of enzyme, but also restricted the catalytic ability of enzyme, which dramatically influenced enzymatic hydrolysis. Increasing the proportion of EG in cellulase cocktails or engineering "weak lignin adsorbed" EG was necessary to relieve the influence of lignin adsorption on hydrolysis. LA - English DB - MTMT ER - TY - JOUR AU - Nitsos, Christos AU - Matsakas, Leonidas AU - Triantafyllidis, Kostas AU - Rova, Ulrika AU - Christakopoulos, Paul TI - Investigation of different pretreatment methods of Mediterranean-type ecosystem agricultural residues: characterisation of pretreatment products, high-solids enzymatic hydrolysis and bioethanol production JF - BIOFUELS J2 - BIOFUELS-UK VL - 9 PY - 2018 IS - 5 SP - 545 EP - 558 PG - 14 SN - 1759-7269 DO - 10.1080/17597269.2017.1378988 UR - https://m2.mtmt.hu/api/publication/30565452 ID - 30565452 AB - Agricultural and agro-industrial lignocellulosic residues represent an important renewable resource for the production of fuels and chemicals towards a bio-based economy. Olive pruning, vineyard pruning and almond shells are important residues from agricultural activities in Mediterranean-type ecosystems. In the current work, bioethanol production from the above three types of agro-residues was studied, focusing on the effect of different pretreatment methods on enzymatic saccharrification efficiency of cellulose and production of second-generation bioethanol. Dilute acid, hydrothermal and steam explosion pretreatments were compared in order to remove hemicellulose and facilitate the subsequent enzymatic hydrolysis of the hemicellulose-deficient biomass to glucose. Enzymatic hydrolysis was performed in a free-fall mixing reactor enabling high solids loading of 23% w/w. This allowed hydrolysis of up to 67% of available cellulose in almond shells and close to 50% in olive pruning samples, and facilitated high ethanol production in the subsequent fermentation step; the highest ethanol concentrations achieved were 47.8 g/L for almond shells after steam explosion and 42 g/L for hydrothermally pretreated olive pruning residue. LA - English DB - MTMT ER - TY - JOUR AU - Parnthong, Jatuporn AU - Kungsanant, Suratsawadee AU - Chavadej, Sumaeth TI - The Influence of Nonionic Surfactant Adsorption on Enzymatic Hydrolysis of Oil Palm Fruit Bunch JF - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY J2 - APPL BIOCHEM BIOTECH VL - 186 PY - 2018 IS - 4 SP - 895 EP - 908 PG - 14 SN - 0273-2289 DO - 10.1007/s12010-018-2783-6 UR - https://m2.mtmt.hu/api/publication/30565431 ID - 30565431 AB - Nonionic surfactants have been utilized to improve the enzymatic hydrolysis of lignocellulosic materials. However, the role of surfactant adsorption affecting enzymatic hydrolysis has not been elaborated well. In this work, nonionic surfactants differing in their molecular structures, namely the polyoxyethylene sorbitan monooleate (Tween 80), the secondary alcohol ethoxylate (Tergitol 15-S-9), and the branched alcohol ethoxylate (Tergitol TMN-6), were studied for their effects on the enzymatic hydrolysis of palm fruit bunch (PFB). The PFB was pretreated with a 10% w/v sodium hydroxide solution and then hydrolyzed using the cellulase enzyme from Trichoderma reesei (ATCC 26921) at 50 degrees C and pH5. The optimal conditions providing similar yields of reducing sugar required Tween 80 and Tergitol TMN-6 at 0.25% w/v, while Tergitol 15-S-9 was required at 0.1% w/v. All the surfactants improved the enzymatic conversion efficiency and reduced unproductive binding of the enzyme to lignin. In addition, the adsorption isotherm of cellulase was fit well by the Freundlich isotherm, while adsorption of the three nonionic surfactants agreed well with the Langmuir isotherm. Adsorption capacities of the three nonionic surfactants were consistent with their enhancement efficiencies in hydrolysis. The critical micelle concentration was observed as a key property of nonionic surfactant for adsorption capacity. LA - English DB - MTMT ER - TY - JOUR AU - Zain, Masniroszaime Md AU - Mohammad, Abdul Wahab AU - Harun, Shuhaida AU - Fauzi, Nurul Aina AU - Hairom, Nur Hanis Hayati TI - Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 122 PY - 2018 SP - 617 EP - 626 PG - 10 SN - 0926-6690 DO - 10.1016/j.indcrop.2018.06.037 UR - https://m2.mtmt.hu/api/publication/30565457 ID - 30565457 AB - Due to an increasing demand for more sustainable and renewable resources, there has been strong interest in utilizing biomass as a source for cleaner production of energy and chemicals. In this work, the lignocellulosic elements of oil palm frond (OPF) biomass were assessed as an alternate sugar feedstock for biofuel and bioproducts production. At present, long hydrolysis times and high enzymatic loadings hinder commercialisation and large-scale utilisation of enzymatic hydrolysis of lignocellulosic biomass. Thus, various process parameters of enzymatic hydrolysis of alkaline OPF fibre were investigated in an attempt to improve process performance. In this study, OPF biomass was pretreated with 4.42% NaOH at 100 degrees C for 58.31 min, resulting in significant disruption as characterised by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Alkaline pretreatment of OPF biomass improved enzymatic biodegradability, and glucan recovery by the Cellic Ctec2 enzyme was more effective than the conventional Celluclast 1.5 L cellulase enzyme. Synergistic effects of stirring speed, surfactant Triton X-100 loading, and B-glucosidase supplement on enzymatic hydrolysis were assessed using statistical experimental design. Under optimal conditions (450 rpm, 1.31%, and 0.14 pNPGU/FPU), 88% conversion of glucan was obtained from alkaline OPF, which is equivalent to the conversion from commercial cellulose (microcrystalline cellulose, MCC). Enzymatic hydrolysis of pretreated OPF was further improved at high agitation speeds. Synergy between agitation speed and surfactant loading interactions with beta-glucosidase supplement enhanced glucose production due to the efficient mixing and availability of cellulose to be adsorbed by cellulase. LA - English DB - MTMT ER - TY - JOUR AU - Ahmed, Samia A AU - Mostafa, Faten A AU - Helmy, Wafaa A AU - Abdel-Naby, Mohamed A TI - Improvement of bacterial alpha-amylase production and application using two steps statistical factorial design JF - BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY J2 - BIOCAT AGRICULT BIOTECH VL - 10 PY - 2017 SP - 224 EP - 233 PG - 10 SN - 1878-8181 DO - 10.1016/j.bcab.2017.03.004 UR - https://m2.mtmt.hu/api/publication/26756723 ID - 26756723 LA - English DB - MTMT ER - TY - JOUR AU - Arslan, Baran AU - Egerton, Kirstin AU - Zhang, Xiao AU - Abu-Lail, Nehal I TI - Effects of the Surface Morphology and Conformations of Lignocellulosic Biomass Biopolymers on Their Nanoscale Interactions with Hydrophobic Self-Assembled Monolayers JF - LANGMUIR J2 - LANGMUIR VL - 33 PY - 2017 IS - 27 SP - 6857 EP - 6868 PG - 12 SN - 0743-7463 DO - 10.1021/acs.langmuir.7b01470 UR - https://m2.mtmt.hu/api/publication/26756720 ID - 26756720 LA - English DB - MTMT ER - TY - JOUR AU - Bandyopadhyay, Sulalit AU - Alvi, Muhammad Awais Ashfaq AU - Sharma, Anuvansh AU - Zhu, Kaizheng AU - Kjoniksen, Anna-Lena AU - Nystrom, Bo AU - Glomm, Wilhelm Robert TI - Influence of polymer coating on release of l-dopa from core-shell Fe@Au nanoparticle systems JF - COLLOID AND POLYMER SCIENCE J2 - COLLOID POLYM SCI VL - 295 PY - 2017 IS - 2 SP - 391 EP - 402 PG - 12 SN - 0303-402X DO - 10.1007/s00396-017-4015-y UR - https://m2.mtmt.hu/api/publication/26571734 ID - 26571734 LA - English DB - MTMT ER - TY - JOUR AU - Chang, Siyuan AU - Guo, Yalan AU - Wu, Bin AU - He, Bingfang TI - Extracellular expression of alkali tolerant xylanase from Bacillus subtilis Lucky9 in E. coli and application for xylooligosaccharides production from agro-industrial waste JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 96 PY - 2017 SP - 249 EP - 256 PG - 8 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2016.11.032 UR - https://m2.mtmt.hu/api/publication/26571732 ID - 26571732 LA - English DB - MTMT ER - TY - JOUR AU - Florencio, Camila AU - Badino, Alberto Colli AU - Farinas, Cristiane Sanchez TI - CURRENT CHALLENGES ON THE PRODUCTION AND USE OF CELLULOLYTIC ENZYMES IN THE HYDROLYSIS OF LIGNOCELLULOSIC BIOMASS JF - QUIMICA NOVA J2 - QUIM NOVA VL - 40 PY - 2017 IS - 9 SP - 1082 EP - 1093 PG - 12 SN - 0100-4042 DO - 10.21577/0100-4042.20170104 UR - https://m2.mtmt.hu/api/publication/27107452 ID - 27107452 LA - Portuguese DB - MTMT ER - TY - JOUR AU - Jiang, Feng AU - Qian, Chen AU - Esker, Alan R AU - Roman, Maren TI - Effect of Nonionic Surfactants on Dispersion and Polar Interactions in the Adsorption of Cellulases onto Lignin JF - JOURNAL OF PHYSICAL CHEMISTRY B J2 - J PHYS CHEM B VL - 121 PY - 2017 IS - 41 SP - 9607 EP - 9620 PG - 14 SN - 1520-6106 DO - 10.1021/acs.jpcb.7b07216 UR - https://m2.mtmt.hu/api/publication/27088896 ID - 27088896 LA - English DB - MTMT ER - TY - JOUR AU - Jorgensen, Henning AU - Pinelo, Manuel TI - Enzyme recycling in lignocellulosic biorefineries JF - BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR J2 - BIOFUEL BIOPROD BIOR VL - 11 PY - 2017 IS - 1 SP - 150 EP - 167 PG - 18 SN - 1932-104X DO - 10.1002/bbb.1724 UR - https://m2.mtmt.hu/api/publication/26571735 ID - 26571735 LA - English DB - MTMT ER - TY - JOUR AU - Kellock, Miriam AU - Rahikainen, Jenni AU - Marjamaa, Kaisa AU - Kruus, Kristiina TI - Lignin-derived inhibition of monocomponent cellulases and a xylanase in the hydrolysis of lignocellulosics JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 232 PY - 2017 SP - 183 EP - 191 PG - 9 SN - 0960-8524 DO - 10.1016/j.biortech.2017.01.072 UR - https://m2.mtmt.hu/api/publication/26756722 ID - 26756722 N1 - Megjegyzés-26461170 N1 Funding details: Fortums Stiftelse N1 Funding text: This work was supported by the Fortum Foundation, Finland. Dr. Arja Paananen is kindly thanked for guidance with AFM imaging and QCM-D measurements. Henri Kellock is gratefully thanked for assistance with setting up the Rosetta software. Klaus Niemelä is thanked for the GC/MS analysis. LA - English DB - MTMT ER - TY - JOUR AU - Lin, Xuliang AU - Lou, Hongming AU - Qiu, Xueqing AU - Pang, Yuxia AU - Yang, Dongjie TI - Effect of sodium dodecyl sulfate and cetyltrimethylammonium bromide catanionic surfactant on the enzymatic hydrolysis of Avicel and corn stover JF - CELLULOSE J2 - CELLULOSE VL - 24 PY - 2017 IS - 2 SP - 669 EP - 676 PG - 8 SN - 0969-0239 DO - 10.1007/s10570-016-1186-5 UR - https://m2.mtmt.hu/api/publication/26571733 ID - 26571733 LA - English DB - MTMT ER - TY - JOUR AU - Li, Xiang AU - Zheng, Yi TI - Lignin-enzyme interaction: Mechanism, mitigation approach, modeling, and research prospects JF - BIOTECHNOLOGY ADVANCES J2 - BIOTECHNOL ADV VL - 35 PY - 2017 IS - 4 SP - 466 EP - 489 PG - 24 SN - 0734-9750 DO - 10.1016/j.biotechadv.2017.03.010 UR - https://m2.mtmt.hu/api/publication/26756721 ID - 26756721 N1 - Cited By :23 Export Date: 30 January 2019 CODEN: BIADD Correspondence Address: Zheng, Y.; Department of Environmental Engineering and Earth Sciences, Clemson University, 342 Computer Court, United States; email: zheng9@clemson.edu Chemicals/CAS: hydrolase, 9027-41-2; lignin, 9005-53-2; lignocellulose, 11132-73-3; Biofuels; Hydrolases; Lignin; lignocellulose Cited By :38 Export Date: 5 September 2019 CODEN: BIADD Correspondence Address: Zheng, Y.; Department of Environmental Engineering and Earth Sciences, Clemson University, 342 Computer Court, United States; email: zheng9@clemson.edu Chemicals/CAS: hydrolase, 9027-41-2; lignin, 9005-53-2; lignocellulose, 11132-73-3; Biofuels; Hydrolases; Lignin; lignocellulose LA - English DB - MTMT ER - TY - JOUR AU - Rocha-Martin, Javier AU - Martinez-Bernal, Claudio AU - Perez-Cobas, Yolanda AU - Reyes-Sosa, Francisco Manuel AU - Garcia, Bruno Diez TI - Additives enhancing enzymatic hydrolysis of lignocellulosic biomass JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 244 PY - 2017 SP - 48 EP - 56 PG - 9 SN - 0960-8524 DO - 10.1016/j.biortech.2017.06.132 UR - https://m2.mtmt.hu/api/publication/27088894 ID - 27088894 LA - English DB - MTMT ER - TY - JOUR AU - Sipponen, Mika Henrikki AU - Rahikainen, Jenni AU - Leskinen, Timo AU - Pihlajaniemi, Ville AU - Mattinen, Maija-Liisa AU - Lange, Heiko AU - Crestini, Claudia AU - Osterberg, Monika TI - Structural changes of lignin in biorefinery pretreatments and consequences to enzyme-lignin interactions JF - NORDIC PULP & PAPER RESEARCH JOURNAL J2 - NORD PULP PAP RES J VL - 32 PY - 2017 IS - 4 SP - 550 EP - 571 PG - 22 SN - 0283-2631 DO - 10.3183/NPPRJ-2017-32-04-p550-571 UR - https://m2.mtmt.hu/api/publication/27088899 ID - 27088899 LA - English DB - MTMT ER - TY - JOUR AU - Smit, A T AU - Huijgen, W J J TI - The promotional effect of water-soluble extractives on the enzymatic cellulose hydrolysis of pretreated wheat straw JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 243 PY - 2017 SP - 994 EP - 999 PG - 6 SN - 0960-8524 DO - 10.1016/j.biortech.2017.07.072 UR - https://m2.mtmt.hu/api/publication/27088895 ID - 27088895 LA - English DB - MTMT ER - TY - JOUR AU - Yamamoto, Yoko AU - Cheng, Ningning AU - Koda, Keiichi AU - Igarashi, Kiyohiko AU - Tamai, Yutaka AU - Uraki, Yasumitsu TI - Association of amphipathic lignin derivatives with cellobiohydrolase groups improves enzymatic saccharification of lignocellulosics JF - CELLULOSE J2 - CELLULOSE VL - 24 PY - 2017 IS - 4 SP - 1849 EP - 1862 PG - 14 SN - 0969-0239 DO - 10.1007/s10570-017-1214-0 UR - https://m2.mtmt.hu/api/publication/26571731 ID - 26571731 LA - English DB - MTMT ER - TY - JOUR AU - Zhao, Chen AU - Deng, Lu AU - Fang, Hao AU - Chen, Shaolin TI - Microbial oil production by Mortierella isabellina from corn stover under different pretreatments JF - RSC ADVANCES J2 - RSC ADV VL - 7 PY - 2017 IS - 89 SP - 56239 EP - 56246 PG - 8 SN - 2046-2069 DO - 10.1039/c7ra11900c UR - https://m2.mtmt.hu/api/publication/27088898 ID - 27088898 LA - English DB - MTMT ER - TY - JOUR AU - Huron, Maite AU - Hudebine, Damien AU - Ferreira, Nicolas Lopes AU - Lachenal, Dominique TI - Impact of delignification on the morphology and the reactivity of steam exploded wheat straw JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 79 PY - 2016 SP - 104 EP - 109 PG - 6 SN - 0926-6690 DO - 10.1016/j.indcrop.2015.10.040 UR - https://m2.mtmt.hu/api/publication/25668013 ID - 25668013 LA - English DB - MTMT ER - TY - JOUR AU - Ivetic, Darjana Z AU - Antov, Mirjana G TI - THE IMPACT OF PRETREATMENTS ON CELLULOSE FROM SUGAR BEET SHREDS AND ITS SUSCEPTIBILITY TO ENZYMATIC HYDROLYSIS JF - CELLULOSE CHEMISTRY AND TECHNOLOGY J2 - CELL CHEM TECHNOL VL - 50 PY - 2016 IS - 1 SP - 139 EP - 146 PG - 8 SN - 0576-9787 UR - https://m2.mtmt.hu/api/publication/26046658 ID - 26046658 LA - English DB - MTMT ER - TY - JOUR AU - Karnaouri, Anthi AU - Matsakas, Leonidas AU - Topakas, Evangelos AU - Rova, Ulrike AU - Christakopoulos, Paul TI - Development of Thermophilic Tailor-Made Enzyme Mixtures for the Bioconversion of Agricultural and Forest Residues JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 7 PY - 2016 PG - 14 SN - 1664-302X DO - 10.3359/fmicb.2016.00177 UR - https://m2.mtmt.hu/api/publication/25668009 ID - 25668009 LA - English DB - MTMT ER - TY - JOUR AU - Liu, Hao AU - Sun, Jianliang AU - Leu, Shao-Yuan AU - Chen, Shicheng TI - Toward a fundamental understanding of cellulase-lignin interactions in the whole slurry enzymatic saccharification process JF - BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR J2 - BIOFUEL BIOPROD BIOR VL - 10 PY - 2016 IS - 5 SP - 648 EP - 663 PG - 16 SN - 1932-104X DO - 10.1002/bbb.1670 UR - https://m2.mtmt.hu/api/publication/26233658 ID - 26233658 LA - English DB - MTMT ER - TY - JOUR AU - Li, Yanfei AU - Sun, Zongping AU - Ge, Xiaoyan AU - Zhang, Junhua TI - Effects of lignin and surfactant on adsorption and hydrolysis of cellulases on cellulose JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 9 PY - 2016 PG - 9 SN - 1754-6834 DO - 10.1186/s13068-016-0434-0 UR - https://m2.mtmt.hu/api/publication/25668012 ID - 25668012 LA - English DB - MTMT ER - TY - JOUR AU - Petridis, Loukas AU - Smith, Jeremy C TI - Conformations of Low-Molecular-Weight Lignin Polymers in Water JF - CHEMSUSCHEM J2 - CHEMSUSCHEM VL - 9 PY - 2016 IS - 3 SP - 289 EP - 295 PG - 7 SN - 1864-5631 DO - 10.1002/cssc.201501350 UR - https://m2.mtmt.hu/api/publication/25668010 ID - 25668010 LA - English DB - MTMT ER - TY - JOUR AU - Saini, Jitendra Kumar AU - Patel, Anil Kumar AU - Adsul, Mukund AU - Singhania, Reeta Rani TI - Cellulase adsorption on lignin: A roadblock for economic hydrolysis of biomass JF - RENEWABLE ENERGY J2 - RENEW ENERGY VL - 98 PY - 2016 IS - Trivandrum SP - 29 EP - 42 PG - 14 SN - 0960-1481 DO - 10.1016/j.renene.2016.03.089 UR - https://m2.mtmt.hu/api/publication/26399778 ID - 26399778 LA - English DB - MTMT ER - TY - JOUR AU - Sakdaronnarong, Chularat AU - Saengsawang, Arisarak AU - Siriyutta, Asanee AU - Jonglertjunya, Woranart AU - Nasongkla, Norased AU - Laosiripojana, Navadol TI - An integrated system for fractionation and hydrolysis of sugarcane bagasse using heterogeneous catalysts in aqueous biphasic system JF - CHEMICAL ENGINEERING JOURNAL J2 - CHEM ENG J VL - 285 PY - 2016 SP - 144 EP - 156 PG - 13 SN - 1385-8947 DO - 10.1016/j.cej.2015.09.098 UR - https://m2.mtmt.hu/api/publication/25668011 ID - 25668011 LA - English DB - MTMT ER - TY - JOUR AU - Strobel, Kathryn L AU - Pfeiffer, Katherine A AU - Blanch, Harvey W AU - Clark, Douglas S TI - Engineering Cel7A carbohydrate binding module and linker for reduced lignin inhibition JF - BIOTECHNOLOGY AND BIOENGINEERING J2 - BIOTECHNOL BIOENG VL - 113 PY - 2016 IS - 6 SP - 1369 EP - 1374 PG - 6 SN - 0006-3592 DO - 10.1002/bit.25889 UR - https://m2.mtmt.hu/api/publication/26046657 ID - 26046657 LA - English DB - MTMT ER - TY - JOUR AU - Zhang, Haiyan AU - Chen, Longjian AU - Lu, Minsheng AU - Li, Junbao AU - Han, Lujia TI - A novel film-pore-surface diffusion model to explain the enhanced enzyme adsorption of corn stover pretreated by ultrafine grinding JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 9 PY - 2016 PG - 12 SN - 1754-6834 DO - 10.1186/s13068-016-0602-2 UR - https://m2.mtmt.hu/api/publication/26233659 ID - 26233659 LA - English DB - MTMT ER - TY - JOUR AU - Zheng, Yi AU - Zhang, Ruihong AU - Pan, Zhongli TI - Investigation of adsorption kinetics and isotherm of cellulase and beta-glucosidase on lignocellulosic substrates JF - BIOMASS & BIOENERGY J2 - BIOMASS BIOENERGY VL - 91 PY - 2016 SP - 1 EP - 9 PG - 9 SN - 0961-9534 DO - 10.1016/j.biombioe.2016.04.014 UR - https://m2.mtmt.hu/api/publication/26233660 ID - 26233660 LA - English DB - MTMT ER - TY - JOUR AU - Zheng, Yi AU - Xiao, Rui AU - Roberts, Mark TI - Polymer-enhanced enzymatic microalgal cell disruption for lipid and sugar recovery JF - ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS J2 - ALGAL RES VL - 14 PY - 2016 SP - 100 EP - 108 PG - 9 SN - 2211-9264 DO - 10.1016/j.algal.2016.01.010 UR - https://m2.mtmt.hu/api/publication/25668008 ID - 25668008 LA - English DB - MTMT ER - TY - JOUR AU - Chen, Liheng AU - Chen, Rong AU - Fu, Shiyu TI - FeCl3 Pretreatment of Three Lignocellulosic Biomass for Ethanol Production JF - ACS SUSTAINABLE CHEMISTRY & ENGINEERING J2 - ACS SUSTAIN CHEM ENG VL - 3 PY - 2015 IS - 8 SP - 1794 EP - 1800 PG - 7 SN - 2168-0485 DO - 10.1021/acssuschemeng.5b00377 UR - https://m2.mtmt.hu/api/publication/25668022 ID - 25668022 LA - English DB - MTMT ER - TY - JOUR AU - Fritz, Consuelo AU - Ferrer, Ana AU - Salas, Carlos AU - Jameel, Hasan AU - Rojas, Orlando J TI - Interactions between Cellulolytic Enzymes with Native, Autohydrolysis, and Technical Lignins and the Effect of a Polysorbate Amphiphile in Reducing Nonproductive Binding JF - BIOMACROMOLECULES J2 - BIOMACROMOLECULES VL - 16 PY - 2015 IS - 12 SP - 3878 EP - 3888 PG - 11 SN - 1525-7797 DO - 10.1021/acs.biomac.5b01203 UR - https://m2.mtmt.hu/api/publication/25668015 ID - 25668015 LA - English DB - MTMT ER - TY - JOUR AU - Ghosh, Debashish AU - Dasgupta, Diptarka AU - Agrawal, Deepti AU - Kaul, Savita AU - Adhikari, Dilip Kumar AU - Kurmi, Akhilesh Kumar AU - Arya, Pankaj K AU - Bangwal, Dinesh AU - Negi, Mahendra Singh TI - Fuels and Chemicals from Lignocellulosic Biomass: An Integrated Biorefinery Approach JF - ENERGY AND FUELS J2 - ENERG FUEL VL - 29 PY - 2015 IS - 5 SP - 3149 EP - 3157 PG - 9 SN - 0887-0624 DO - 10.1021/acs.energyfuels.5b00144 UR - https://m2.mtmt.hu/api/publication/25668025 ID - 25668025 LA - English DB - MTMT ER - TY - JOUR AU - Hsieh, Chia-wen C AU - Cannella, David AU - Jorgensen, Henning AU - Felby, Claus AU - Thygesen, Lisbeth G TI - Cellobiohydrolase and endoglucanase respond differently to surfactants during the hydrolysis of cellulose JF - 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10 SN - 0006-3592 DO - 10.1002/bit.25359 UR - https://m2.mtmt.hu/api/publication/24662590 ID - 24662590 LA - English DB - MTMT ER - TY - JOUR AU - Li, Kena AU - Wang, Xiao AU - Wang, Jingfeng AU - Zhang, Junhua TI - Benefits from additives and xylanase during enzymatic hydrolysis of bamboo shoot and mature bamboo JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 192 PY - 2015 SP - 424 EP - 431 PG - 8 SN - 0960-8524 DO - 10.1016/j.biortech.2015.05.100 UR - https://m2.mtmt.hu/api/publication/25668021 ID - 25668021 LA - English DB - MTMT ER - TY - JOUR AU - Lin, Xuliang AU - Qiu, Xueqing AU - Yuan, Long AU - Li, Zihao AU - Lou, Hongming AU - Zhou, Mingsong AU - Yang, Dongjie TI - Lignin-based polyoxyethylene ether enhanced enzymatic hydrolysis of lignocelluloses by dispersing cellulase aggregates JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 185 PY - 2015 SP - 165 EP - 170 PG - 6 SN - 0960-8524 DO - 10.1016/j.biortech.2015.02.067 UR - https://m2.mtmt.hu/api/publication/25668024 ID - 25668024 LA - English DB - MTMT ER - TY - JOUR AU - Li, Yanfei AU - Ge, Xiaoyan AU - Sun, Zongping AU - Zhang, Junhua TI - Effect of additives on adsorption and desorption behavior of xylanase on acid-insoluble lignin from corn stover and wheat straw JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 186 PY - 2015 SP - 316 EP - 320 PG - 5 SN - 0960-8524 DO - 10.1016/j.biortech.2015.03.058 UR - https://m2.mtmt.hu/api/publication/25668023 ID - 25668023 LA - English DB - MTMT ER - TY - JOUR AU - Mason, P Michael AU - Glover, Katherine AU - Smith, J Andrew C AU - Willis, Kathy J AU - Woods, Jeremy AU - Thompson, Ian P TI - The potential of CAM crops as a globally significant bioenergy resource: moving from 'fuel or food' to 'fuel and more food' JF - ENERGY & ENVIRONMENTAL SCIENCE J2 - ENERG ENVIRON SCI VL - 8 PY - 2015 IS - 8 SP - 2320 EP - 2329 PG - 10 SN - 1754-5692 DO - 10.1039/c5ee00242g UR - https://m2.mtmt.hu/api/publication/25668030 ID - 25668030 LA - English DB - MTMT ER - TY - JOUR AU - Nasirpour, Niloofar AU - Mousavi, Seyyed Mohammad AU - Shojaosadati, Seyed Abbas TI - A study on cell surface hydrophobicity, growth and metabolism of Zymomonas mobilis influenced by PEG as a pretreatment agent JF - RSC ADVANCES J2 - RSC ADV VL - 5 PY - 2015 IS - 60 SP - 48176 EP - 48180 PG - 5 SN - 2046-2069 DO - 10.1039/c5ra03181h UR - https://m2.mtmt.hu/api/publication/25668028 ID - 25668028 LA - English DB - MTMT ER - TY - JOUR AU - Oliva-Taravilla, Alfredo AU - Tomas-Pejo, Elia AU - Demuez, Marie AU - Gonzalez-Fernandez, Cristina AU - Ballesteros, Mercedes TI - Inhibition of Cellulose Enzymatic Hydrolysis by Laccase-Derived Compounds from Phenols JF - BIOTECHNOLOGY PROGRESS J2 - BIOTECHNOL PROGR VL - 31 PY - 2015 IS - 3 SP - 700 EP - 706 PG - 7 SN - 8756-7938 DO - 10.1002/btpr.2068 UR - https://m2.mtmt.hu/api/publication/25668026 ID - 25668026 LA - English DB - MTMT ER -