@article{MTMT:35304179,
	title = {Non-ionic surfactant PEG: Enhanced cutinase-catalyzed hydrolysis of polyethylene terephthalate},
	url = {https://m2.mtmt.hu/api/publication/35304179},
	author = {Feng, Jundan and Li, Huimin and Lu, Yuzheng and Li, Rong and Cavaco-paulo, Artur and Fu, Jiajia},
	doi = {10.1016/j.ijbiomac.2024.133049},
	journal-iso = {INT J BIOL MACROMOL},
	journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
	volume = {273},
	unique-id = {35304179},
	issn = {0141-8130},
	keywords = {PET; PEG; enzymatic hydrolysis; Biocatalysts; HIC; PET hydrolysis products},
	year = {2024},
	eissn = {1879-0003}
}

@article{MTMT:35304178,
	title = {Polymer and micellar catalysis in D-fructose oxidation by imidazolium fluorochromate: A kinetic and mechanistic study},
	url = {https://m2.mtmt.hu/api/publication/35304178},
	author = {Tomar, Ashish and Arora, Unik and Singh, Jai Veer},
	doi = {10.1016/j.jics.2024.101267},
	journal-iso = {J INDIAN CHEM SOC},
	journal = {JOURNAL OF THE INDIAN CHEMICAL SOCIETY},
	volume = {101},
	unique-id = {35304178},
	issn = {0019-4522},
	keywords = {MECHANISM; KINETICS; OXIDATION; Sodium Dodecyl Sulfate; D -fructose; Imidazolium fluorochromate; Polyethylene glycol (PEG)-400},
	year = {2024}
}

@article{MTMT:34278449,
	title = {Synthesis of lignin-based biosurfactant derived from kraft black liquor and its effect on enzymatic hydrolysis of pretreated biomass},
	url = {https://m2.mtmt.hu/api/publication/34278449},
	author = {Solihat, Nissa Nurfajrin and Pramasari, Dwi Ajias and Laksana, Raden Permana Budi and Restu, Witta Kartika and Ghozali, Muhammad and Triwulandari, Evi and Fatriasari, Widya and Watanabe, Takashi},
	doi = {10.1016/j.scp.2023.101152},
	journal-iso = {SUSTAIN CHEM PHARM},
	journal = {SUSTAINABLE CHEMISTRY AND PHARMACY},
	volume = {34},
	unique-id = {34278449},
	issn = {2352-5541},
	abstract = {Lignin, the main constituent in black liquor of up to 40%, can be converted into a value-added commodity such as a biosurfactant. In this study, lignin was extracted from the Acacia mangium black liquor kraft by acid precipitation (L1S) and ethanol fractionation (L2S). Subsequently, dried lignin was characterized physically and chemically. Biosurfactant was obtained by reacting lignin with polyvinyl alcohol (PVA). To evaluate the fruitfulness of biosurfactants on enzymatic hydrolysis, some optimizations have been conducted: volume ratio PVA: lignin, concentration PVA: lignin, different lignin source, substrate variation, and the effect of an initiator (potassium peroxodisulfate). Lignin by ethanol fractionation resulted in higher lignin content, methoxyl, molecular weight, and syringil but lower particle size and ash content. However, the thermal sta-bility of these two lignins is similar. The ratio of PVA: lignin gave a positive effect on boosting re-ducing sugar yield (RSY) while the high concentration of PVA: lignin had the opposite effect. The best volume of PVA: lignin was 250 & mu;L with a concentration of biosurfactant 0.4% to get the high-est RSY per biomass about 80%. Meanwhile, biosurfactant-based L2S released more RSY than L1S and lignin sigma Aldrich. Kraft process of sweet sorghum bagasse (SSB) obtained more sugar than other substrates such as an alkaline autoclave SSB, jabon soda pulp, and microwave-assisted sul-furic acid pretreated of oil palm empty fruit bunch (EFB). The initiator addition during synthesiz-ing of biosurfactant was predicted to contribute to achieving higher RSY compared to that of that non-initiator addition. This study demonstrates the ability of lignin-based biosurfactants to im-prove enzymatic hydrolysis yet the best condition was depending on the ratio, concentration, and volume of PVA: lignin, lignin types, substrates, and initiator.},
	keywords = {enzymatic hydrolysis; Reducing sugar yield; Black liquor; Lignin -based biosurfactant},
	year = {2023},
	eissn = {2352-5541},
	orcid-numbers = {Solihat, Nissa Nurfajrin/0000-0002-7959-845X}
}

@article{MTMT:33662094,
	title = {Beneficial effect of surfactant in adsorption/desorption of lignocellulose-degrading enzymes on/from lignin with different structure},
	url = {https://m2.mtmt.hu/api/publication/33662094},
	author = {Wang, J. and Xiao, W. and Zhang, J. and Quan, X. and Chu, J. and Meng, X. and Pu, Y. and Ragauskas, A.J.},
	doi = {10.1016/j.indcrop.2022.115904},
	journal-iso = {IND CROP PROD},
	journal = {INDUSTRIAL CROPS AND PRODUCTS},
	volume = {191},
	unique-id = {33662094},
	issn = {0926-6690},
	year = {2023},
	eissn = {1872-633X}
}

@article{MTMT:34609150,
	title = {Effects of Metal Chloride Salt Pretreatment and Additives on Enzymatic Hydrolysis of Poplar},
	url = {https://m2.mtmt.hu/api/publication/34609150},
	author = {Zhao, Chenbiao and Mai, Shenyi and Fan, Meishan and Xie, Jun and Zhang, Hongdan},
	doi = {10.3390/fermentation9121022},
	journal-iso = {FERMENTATION-BASEL},
	journal = {FERMENTATION},
	volume = {9},
	unique-id = {34609150},
	abstract = {Metal chloride salt pretreatment was performed to isolate and convert cellulose to glucose from poplar. A glucose yield of 82.0% +/- 0.7 was achieved after 0.05 mol/L AlCl3 pretreatment conducted at 180 degrees C for 20 min, ascribing to the removal of hemicellulose, the alteration of crystallinity, surface morphology, and the retention of the majority of cellulose. Then, the influence of different additives on glucose yield was assessed, generating the highest glucose yield of 88.5 +/- 0.06 with the addition of PEG 8000. Meanwhile, a similar glucose yield of 82.8% +/- 0.3 could be obtained with PEG 8000 when hydrolysis time was reduced by a quarter and enzyme dosage by three-quarters. It can be seen that AlCl3 pretreatment is a viable and efficient pretreatment method for poplar, while the addition of PEG 8000 can enhance the enzymatic efficiency and reduce cellulase loading, ascribing to the reservation of free enzyme and enzyme activity in the supernatant and the reduction in surface tension, which provide an idea to improve the economics of the enzymatic conversion of poplar.},
	keywords = {poplar; ADDITIVES; Glucose yield; AlCl3 pretreatment; increased yield},
	year = {2023},
	eissn = {2311-5637}
}

@article{MTMT:33003572,
	title = {Alleviating Nonproductive Adsorption of Lignin on CBM through the Addition of Cationic Additives for Lignocellulosic Hydrolysis},
	url = {https://m2.mtmt.hu/api/publication/33003572},
	author = {Han, Lijuan and Jiang, Baojie and Wang, Wei and Wang, Gaosheng and Tan, Yinshuang and Niu, Kangle and Fang, Xu},
	doi = {10.1021/acsabm.2c00112},
	journal-iso = {ACS APPL BIO MATER},
	journal = {ACS APPLIED BIO MATERIALS},
	volume = {5},
	unique-id = {33003572},
	issn = {2576-6422},
	abstract = {The nonproductive adsorption of cellulase onto lignin significantly inhibited the enzymatic hydrolysis of lignocellulosic biomass. In this study, we constructed a rapid fluorescence detection (RFD) system, and using this system, we demonstrated that the addition of cationic additives DTAB or polyDADMAC greatly increased the partition coefficients of cellulose/lignin, reduced nonproductive adsorption, and enhanced the hydrolysis efficiency of lignocellulose compared to those of Tweens or PEGs. Moreover, the addition of polyDADMAC and DTAB increased the glucose yield released from the mixture of Avicel and AICS-lignin (MCL) by 16.9 and 20.6%, respectively, and reduced the inhibition rate of lignin by 16.9 and 20.7%, respectively. Interestingly, polyDADMAC or DTAB treatment performed more effectively for the enzymatic hydrolysis of pretreated lignocellulosic biomass, compared with MCL. We confirmed that the reduced hydrophobicity and increased zeta potential of lignin cocontribute to the dampening nonproductive adsorption of lignin. In particular, the zeta potential values of lignin and the partition coefficients of Avicel/lignin with the addition of additives showed a good correlation, suggesting that electrostatic force also plays a crucial role in the adsorbing of cellulase on lignin. This work will be conducive to decreasing the nonproductive binding of cellulase onto lignin and enhancing cellulose conversion.},
	keywords = {Hydrophobicity; ELECTROSTATIC INTERACTION; Lignocellulosic hydrolysis; rapid fluorescence detection system; cationic additives; nonproductive adsorption},
	year = {2022},
	eissn = {2576-6422},
	pages = {2253-2261},
	orcid-numbers = {Fang, Xu/0000-0002-9196-5697}
}

@article{MTMT:33224770,
	title = {Revealing the mechanism of surfactant-promoted enzymatic hydrolysis of dilute acid pretreated bamboo},
	url = {https://m2.mtmt.hu/api/publication/33224770},
	author = {Huang, Caoxing and Zhao, Xiaoxue and Zheng, Yayue and Lin, Wenqian and Lai, Chenhuan and Yong, Qiang and Ragauskas, Arthur J. and Meng, Xianzhi},
	doi = {10.1016/j.biortech.2022.127524},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {360},
	unique-id = {33224770},
	issn = {0960-8524},
	abstract = {To improve the enzymatic digestibility of dilute acid pretreated bamboo residue (DABR), surfactants including PEG 4000 and Tween 80 were added to prevent the non-productive adsorption between residual lignin and enzyme. At the optimal loadings (e.g., 0.2 and 0.3 g surfactant/g lignin), the enzymatic digestibility of DABR improved from 29.4% to 64.6% and 61.6% for PEG 4000 and Tween 80, respectively. Furthermore, the pro-moting mechanism of these surfactants on enzymatic hydrolysis was investigated by real-time surface plasmon resonance (SPR) and fluorescence spectroscopy. Results from SPR analysis showed that Tween 80 outperformed PEG 4000 in terms of dissociating the irreversible cellulase adsorption onto lignin. Fluorescence quenching mechanism revealed that PEG 4000 and Tween 80 intervened the interaction between lignin and cellulase by hydrogen bonds/Van der Waals and hydrophobic action, respectively. This work provided an in-depth under-standing of the mechanisms of PEG 4000 and Tween 80 on enhancing the enzymatic hydrolysis efficiency.},
	keywords = {surface plasmon resonance; enzymatic hydrolysis; surfactant; Fluorescence spectroscopy; Residual lignin},
	year = {2022},
	eissn = {1873-2976}
}

@article{MTMT:33003575,
	title = {Current understanding and optimization strategies for efficient lignin-enzyme interaction: A review},
	url = {https://m2.mtmt.hu/api/publication/33003575},
	author = {Li, Mohan and Jiang, Bo and Wu, Wenjuan and Wu, Shufang and Yang, Yiqin and Song, Junlong and Ahmad, Mehraj and Jin, Yongcan},
	doi = {10.1016/j.ijbiomac.2021.11.188},
	journal-iso = {INT J BIOL MACROMOL},
	journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
	volume = {195},
	unique-id = {33003575},
	issn = {0141-8130},
	abstract = {From energy perspective, with abundant polysaccharides (45-85%), the renewable lignocellulosic is recognized as the 2nd generation feedstock for bioethanol and bio-based products production. Enzymatic hydrolysis is a critical pathway to yield fermentable monosaccharides from pretreated substrates of lignocellulose. Nevertheless, the lignin presence in lignocellulosic substrates leads to the low substrate enzymatic digestibility ascribed to the nonproductive adsorption. It has been reported that the water-soluble lignin (low molecular weight, sulfonated/ sulfomethylated and graft polymer) enhance the rate of enzymatic digestibility, however, the catalytic mechanism of lignin-enzyme interaction remains elusive. In this review, optimization strategies for enzymatic hydrolysis based on the lignin structural modification, enzyme engineering, and different additives are critically reviewed. Lignin-enzyme interaction mechanism is also discussed (lignin and various cellulases). In addition, the mathematical models and simulation of lignin, cellulose and enzyme aims for promoting an integrated biomassconversion process for sustainable production of value-added biofuels.},
	keywords = {enzymatic hydrolysis; Optimization strategy; lignocellulose; Water-soluble lignin; Lignin-enzyme interaction},
	year = {2022},
	eissn = {1879-0003},
	pages = {274-286}
}

@article{MTMT:33003573,
	title = {Effects of the Addition of Poly(ethylene Glycol) and Non-ionic Surfactants on Pretreatment, Enzymatic Hydrolysis, and Ethanol Fermentation},
	url = {https://m2.mtmt.hu/api/publication/33003573},
	author = {Nogueira, Cleitiane da Costa and de Araujo Padilha, Carlos Eduardo and de Souza Filho, Pedro Ferreira and dos Santos, Everaldo Silvino},
	doi = {10.1007/s12155-021-10388-9},
	journal-iso = {BIOENERG RES},
	journal = {BIOENERGY RESEARCH},
	volume = {15},
	unique-id = {33003573},
	issn = {1939-1234},
	abstract = {The consolidation of cellulosic ethanol on the market is fundamental to mitigate the consumption of fuels from fossil sources and to reduce the impact caused by the large generation of agro-industrial waste. In order to achieve this objective, some challenges of cellulosic ethanol technology must be overcome, including the improvement of the cellulosic ethanol production steps. Several studies propose the use of poly(ethylene glycol) (PEG) and non-ionic surfactants (such as Tween 80, Tween 20, and Triton X-100) as a way to increase cellulosic ethanol titers. The benefits attributed to the PEG and non-ionic surfactants go beyond the increase of the concentration of free cellulases during enzymatic hydrolysis. Successful cases of pretreatments of lignocellulosic biomasses assisted by PEG and surfactants and the detoxification of inhibitor-rich hydrolysates with PEG reveal the existence of a plethora of positive mechanisms. Therefore, the present review article is focused on the benefits and mechanisms involved in the addition of PEG and non-ionic surfactants in the pretreatment, enzymatic hydrolysis, and ethanol fermentation steps. Interactions between additives and lignin as well as schemes based on high PEG concentrations were also discussed in detail.},
	keywords = {HYDROLYSIS; BIOMASS; FERMENTATION; PEG; detoxification; Tween},
	year = {2022},
	eissn = {1939-1242},
	pages = {889-904}
}

@article{MTMT:33224769,
	title = {Enhancement of catalytic activity and alkaline stability of cellobiohydrolase by structure-based protein engineering},
	url = {https://m2.mtmt.hu/api/publication/33224769},
	author = {Prabmark, Kanoknart and Boonyapakron, Katewadee and Bunterngsook, Benjarat and Arunrattanamook, Nattapol and Uengwetwanit, Tanaporn and Chitnumsub, Penchit and Champreda, Verawat},
	doi = {10.1007/s13205-022-03339-4},
	journal-iso = {3 BIOTECH},
	journal = {3 BIOTECH},
	volume = {12},
	unique-id = {33224769},
	issn = {2190-572X},
	abstract = {Alkaline cellobiohydrolases have the potential for application in various industries, including pulp processing and laundry where operation under high pH conditions is preferred. In this study, variants of CtCel6A cellobiohydrolase from Chaetomium thermophilum were generated by structural-based protein engineering with the rationale of increasing catalytic activity and alkaline stability. The variants included removal of the carbohydrate-binding module (CBM) and substitution of residues 173 and 200. The CBM-deleted enzyme with Y200F mutation predicted to mediate conformational change at the N-terminal loop demonstrated increased alkaline stability at 60 degrees C, pH 8.0 for 24 h up to 2.25-fold compared with the wild-type enzyme. Another CBM-deleted enzyme with L173E mutation predicted to induce a new hydrogen bond in the substrate-binding cleft showed enhanced hydrolysis yield of pretreated sugarcane trash up to 4.65-fold greater than that of the wild-type enzyme at the pH 8.0. The variant enzymes could thus be developed for applications on cellulose hydrolysis and plant fiber modification operated under alkaline conditions.},
	keywords = {Pulp and paper; Cellobiohydrolase; Alkaline stability; Structure-based protein engineering},
	year = {2022},
	eissn = {2190-5738},
	orcid-numbers = {Prabmark, Kanoknart/0000-0003-0681-2176}
}

@article{MTMT:32391978,
	title = {Protective effects of non-catalytic proteins on endoglucanase activity at air and lignin interfaces},
	url = {https://m2.mtmt.hu/api/publication/32391978},
	author = {Almeida, Renata M. R. G. and Pimentel, Wagner R. O. and Santos-Rocha, Martha S. R. and Buffo, Mariane M. and Farinas, Cristiane Sanchez and Ximenes, Eduardo A. and Ladisch, Michael R.},
	doi = {10.1002/btpr.3134},
	journal-iso = {BIOTECHNOL PROGR},
	journal = {BIOTECHNOLOGY PROGRESS},
	volume = {37},
	unique-id = {32391978},
	issn = {8756-7938},
	abstract = {The manner in which added non-catalytic proteins during enzymatic hydrolysis of lignocellulosic substrates enhances hydrolysis mechanisms is not completely understood. Prior research has indicated that a reduction in the non-specific adsorption of enzymes on lignin, and deactivation of enzymes exposed to air-liquid interface provide rationale. This work investigated root causes including effects of the air-liquid interface on non-catalytic proteins, and effects of lignin on endoglucanase. Three different experimental designs and three variables (air-liquid interfacial area, the types of lignin (acid or enzymatic lignin), and the presence of non-enzymatic protein (bovine serum albumin [BSA] or soy proteins ) were used. The results showed that acid isolated lignin adsorbed almost all endoglucanase activity initially present in supernatant, independent of air interface conditions (25 or 250 ml flasks) with the presence of BSA preventing this effect. Endoglucanase lost 30%-50% of its activity due to an air-liquid interface in the presence of lignin while addition of non-enzymatic protein helped to preserve this enzyme's activity. Langmuir and Freundlich models applied to experimental data indicated that the adsorption increases with increasing temperature for both endoglucanase and BSA. Adsorption of the enzyme and protein were endothermic with an increase in entropy. These results, combined, show that hydrophobicity plays a strong role in the adsorption of both endoglucanase and BSA on lignin.},
	keywords = {bovine serum albumin; soybean protein; Cellulases; Interfacial area; non‐; productive adsorption},
	year = {2021},
	eissn = {1520-6033}
}

@article{MTMT:32391974,
	title = {Lignin enhances cellulose dissolution in cold alkali},
	url = {https://m2.mtmt.hu/api/publication/32391974},
	author = {Costa, Carolina and Medronho, Bruno and Eivazi, Alireza and Svanedal, Ida and Lindman, Bjorn and Edlund, Hakan and Norgren, Magnus},
	doi = {10.1016/j.carbpol.2021.118661},
	journal-iso = {CARBOHYD POLYM},
	journal = {CARBOHYDRATE POLYMERS},
	volume = {274},
	unique-id = {32391974},
	issn = {0144-8617},
	abstract = {Aqueous sodium hydroxide solutions are extensively used as solvents for lignin in kraft pulping. These are also appealing systems for cellulose dissolution due to their inexpensiveness, ease to recycle and low toxicity. Cellulose dissolution occurs in a narrow concentration region and at low temperatures. Dissolution is often incomplete but additives, such as zinc oxide or urea, have been found to significantly improve cellulose dissolution. In this work, lignin was explored as a possible beneficial additive for cellulose dissolution. Lignin was found to improve cellulose dissolution in cold alkali, extending the NaOH concentration range to lower values. The regenerated cellulose material from the NaOH-lignin solvents was found to have a lower crystallinity and crystallite size than the samples prepared in the neat NaOH and NaOH-urea solvents. Beneficial lignin-cellulose interactions in solution state appear to be preserved under coagulation and regeneration, reducing the tendency of crystallization of cellulose.},
	keywords = {LIGNIN; DISSOLUTION; Cellulose amphiphilicity; NaOH (aq; ) solvent},
	year = {2021},
	eissn = {1879-1344}
}

@article{MTMT:32391977,
	title = {Comparing bioethanol production using buttonwood (Conocarpus erectus) and date palm (Phoenix dactylifera) leaves as raw material},
	url = {https://m2.mtmt.hu/api/publication/32391977},
	author = {Gomaa, Mohamed A. and Al-Makhmari, Moza and Al-Hinai, Mohab Ali},
	doi = {10.1080/17597269.2018.1537207},
	journal-iso = {BIOFUELS-UK},
	journal = {BIOFUELS},
	volume = {12},
	unique-id = {32391977},
	issn = {1759-7269},
	abstract = {A major obstacle of bioethanol production is finding a renewable and cheap feedstock, which still needs to be addressed. This work focuses on comparing bioethanol production from buttonwood and date palm leaves. Furthermore, cellulase activities were compared from 4 newly isolated cellulase producing Bacillus spp. and optimal temperature, pH and metal ion supplementation conditions to enhance the cellulolytic activity were determined. Finally, the bioethanol yields were compared between a Clostridium aciditolerans sp. (hereafter referred to as AK-1) and Saccharomyces cerevisiae for maximum bioethanol yields. Cellulase activity as high as 372 and 192 U/g substrate were obtained using buttonwood and date palm leaves, respectively, under optimal conditions. Using newly isolated Bacillus strain MI-42 for saccharification, efficiencies of 77.8 and 72.5% were obtained from buttonwood and date palm leaves, respectively. Using AK-1, maximum bioethanol yields of 2.07 and 0.85 g/L were obtained from buttonwood and date palm leaves, respectively, while maximum yields using S. cerevisiae reached 2.12 and 0.9 g/L. However, AK-1 required 96 hours to reach maximum yield, while S. cerevisiae needed just 10 hours. Although buttonwood and date palm leaves could be suitable feedstock, further optimization of the fermentation process is still needed to improve bioethanol yields.},
	keywords = {enzyme activity; FERMENTATION; Bacillus; Clostridium; Bioethanol; Cellulase},
	year = {2021},
	eissn = {1759-7277},
	pages = {769-775}
}

@article{MTMT:32391982,
	title = {Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses},
	url = {https://m2.mtmt.hu/api/publication/32391982},
	author = {Gong, Zhenggang and Yang, Guangxu and Song, Junlong and Zheng, Peitao and Liu, Jing and Zhu, Wenyuan and Huang, Liulian and Chen, Lihui and Luo, Xiaolin and Shuai, Li},
	doi = {10.1186/s40643-021-00363-9},
	journal-iso = {BIORES BIOPROC},
	journal = {BIORESOURCES AND BIOPROCESSING},
	volume = {8},
	unique-id = {32391982},
	abstract = {Lignin deposits formed on the surface of pretreated lignocellulosic substrates during acidic pretreatments can non-productively adsorb costly enzymes and thereby influence the enzymatic hydrolysis efficiency of cellulose. In this article, peanut protein (PP), a biocompatible non-catalytic protein, was separated from defatted peanut flour (DPF) as a lignin blocking additive to overcome this adverse effect. With the addition of 2.5 g/L PP in enzymatic hydrolysis medium, the glucose yield of the bamboo substrate pretreated by phenylsulfonic acid (PSA) significantly increased from 38 to 94% at a low cellulase loading of 5 FPU/g glucan while achieving a similar glucose yield required a cellulase loading of 17.5 FPU/g glucan without PP addition. Similar promotion effects were also observed on the n-pentanol-pretreated bamboo and PSA-pretreated eucalyptus substrates. The promoting effect of PP on enzymatic hydrolysis was ascribed to blocking lignin deposits via hydrophobic and/or hydrogen-bonding interactions, which significantly reduced the non-productive adsorption of cellulase onto PSA lignin. Meanwhile, PP extraction also facilitated the utilization of residual DPF as the adhesive for producing plywood as compared to that without protein pre-extraction. This scheme provides a sustainable and viable way to improve the value of woody and agriculture biomass. Peanut protein, a biocompatible non-catalytic protein, can block lignin, improve enzymatic hydrolysis efficiency and thereby facilitate the economics of biorefinery.},
	keywords = {ADSORPTION; PRETREATMENT; enzymatic hydrolysis; BLOCKING; PEANUT PROTEIN; Lignin deposits},
	year = {2021},
	eissn = {2197-4365},
	orcid-numbers = {Luo, Xiaolin/0000-0002-3110-0552}
}

@article{MTMT:33003576,
	title = {Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo},
	url = {https://m2.mtmt.hu/api/publication/33003576},
	author = {Lv, Xianqing and Yang, Guangxu and Gong, Zhenggang and Cheng, Xin and Shuai, Li and Huang, Liulian and Chen, Lihui and Luo, Xiaolin and Liu, Jing},
	doi = {10.3389/fbioe.2021.804456},
	journal-iso = {FRONT BIOENG BIOTECHNOL},
	journal = {FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY},
	volume = {9},
	unique-id = {33003576},
	issn = {2296-4185},
	abstract = {Chemical pretreatment followed by enzymatic hydrolysis has been regarded as a viable way to produce fermentable sugars. Phenylsulfonic acid (PSA) pretreatment could efficiently fractionate the non-cellulosic components (hemicelluloses and lignin) from bamboo and result in increased cellulose accessibility that was 10 times that of untreated bamboo. However, deposited lignin could trigger non-productive adsorption to enzymes, which therefore significantly decreased the enzymatic hydrolysis efficiency of PSA-pretreated bamboo substrates. Herein, poly(N-vinylcaprolactam) (PNVCL), a non-ionic surfactant, was developed as a novel additive for overcoming the non-productive adsorption of lignin during enzymatic hydrolysis. PNVCL was found to be not only more effective than those of commonly used lignosulfonate and polyvinyl alcohol for overcoming the negative effect of lignin, but also comparable to the robust Tween 20 and bovine serum albumin additives. A PNVCL loading at 1.2 g/L during enzymatic hydrolysis of PSA pretreated bamboo substrate could achieve an 80% cellulosic enzymatic conversion and meanwhile reduce the cellulase loading by three times as compared to that without additive. Mechanistic investigations indicated that PNVCL could block lignin residues through hydrophobic interactions and the resultant PNVCL coating resisted the adsorption of cellulase via electrostatic repulsion and/or hydration. This practical method can improve the lignocellulosic enzymatic hydrolysis efficiency and thereby increase the productivity and profitability of biorefinery.},
	keywords = {LIGNIN; enzymatic hydrolysis; Non-productive adsorption; Poly(N-vinylcaprolactam); phenylsulfonic acid},
	year = {2021},
	eissn = {2296-4185}
}

@article{MTMT:32391980,
	title = {Eucalyptus lignin modification for dynamic adsorption with lignocellulose-degradation enzymes dependent on pH values},
	url = {https://m2.mtmt.hu/api/publication/32391980},
	author = {Mou, Hongyan and Wu, Xiao and Huang, Jin and Liu, Yibei and Fan, Huiming},
	doi = {10.1016/j.indcrop.2021.113650},
	journal-iso = {IND CROP PROD},
	journal = {INDUSTRIAL CROPS AND PRODUCTS},
	volume = {169},
	unique-id = {32391980},
	issn = {0926-6690},
	abstract = {The large amount of ineffective adsorption of cellulase on lignin seriously impeded the enzymatic hydrolysis efficiency and reduced the enzyme activity. In this study, the eucalyptus lignin obtained by alkali and hydrotropic methods was used as raw materials to investigate the differences in the dynamic adsorption with different enzymes and enzyme components in detail. The lignin influenced on enzyme activity of different enzymes and enzyme components was determined as well. Furthermore, alkali lignin (AL) and sodium xylene sulfonate lignin (SL) were modified by dimethylamine (DMA) and diethylenetriamine (DETA) through Mannich reaction. By introducing a certain amount of amine groups on lignin to reduce the surface charge and hydrophobicity of lignin for greatly reduction adsorption of cellulase. Under the optimum condition (pH 4.8), the adsorption of cellulase onto DETA-AL-amine was reduced by 96.32 % from 136 mg g-1 to 5 mg g-1. This work suggests that ligninamine has potential applications in reducing enzyme adsorption.},
	keywords = {LIGNIN; Mannich reaction; Cellulase; Dynamic adsorption; Lignin-amine},
	year = {2021},
	eissn = {1872-633X}
}

@article{MTMT:32323125,
	title = {Boosting second-generation ethanol titers from green coconut fiber by using high-concentration polyethylene glycol},
	url = {https://m2.mtmt.hu/api/publication/32323125},
	author = {Nogueira, Cleitiane da Costa and Padilha, Carlos Eduardo de Araujo and dos Santos, Everaldo Silvino},
	doi = {10.1016/j.indcrop.2021.113494},
	journal-iso = {IND CROP PROD},
	journal = {INDUSTRIAL CROPS AND PRODUCTS},
	volume = {166},
	unique-id = {32323125},
	issn = {0926-6690},
	abstract = {Efforts have been made to improve and modify lignocellulosic pretreatments, but this step corresponds to a high cost in second-generation ethanol production. Previous studies have shown that high-concentration polyethylene glycol (PEG) is a possible way to dispense chemical and physicochemical pretreatments and increase ethanol production from lignocellulosic material. However, no operational conditions of this strategy were optimized. The present study investigated the effects of operational conditions on simultaneous saccharification and fermentation (SSF) performance using high PEG concentrations and green coconut fiber (GCF) as a substrate. The high-concentration PEG (150 g.L-1) increased ethanol production compared to the PEG-free, 10, and 50 g.L-1 PEG conditions. Using 20 % (w.v- 1) solid loading, the batch SSF cultivation with PEG 1500 and without supplementary nutrients reached ethanol production and ethanol yield equal to 22.21 g.L-1 and 64.0 %, respectively. The batch SSF cultivation with 30 % (w.v- 1) solid loading presented mass transfer limitations. The addition of salts, yeast extract, or peptone improved ethanol production. Inhibition by ethanol on the Saccharomyces cerevisiae CAT-1 strain was indifferent to the presence of PEG 1500, while the addition of PEG ensured the cellulolytic activity reuse after three fermentation cycles. The fed-batch SSF with PEG facilitated the GCF liquefaction so that it was possible to operate with up to 30 % (w.v- 1) solid loading without problems of viscosity and free water. Using 150 g.L-1 PEG 1500 and enzyme loading equal to 13.3 filter paper unit. g-1, the fed-batch SSF reached 35.1 g.L-1 ethanol at 48 h, representing an increase of 70 % compared to the fed-batch SSF without PEG.},
	keywords = {FERMENTATION; PEG; enzymatic hydrolysis; detoxification; Cellulase},
	year = {2021},
	eissn = {1872-633X}
}

@article{MTMT:32391979,
	title = {Enzymatic hydrolysis and simultaneous saccharification and fermentation of green coconut fiber under high concentrations of ethylene oxide-based polymers},
	url = {https://m2.mtmt.hu/api/publication/32391979},
	author = {Nogueira, Cleitiane da Costa and de Araujo Padilha, Carlos Eduardo and dos Santos, Everaldo Silvino},
	doi = {10.1016/j.renene.2020.10.050},
	journal-iso = {RENEW ENERGY},
	journal = {RENEWABLE ENERGY},
	volume = {163},
	unique-id = {32391979},
	issn = {0960-1481},
	abstract = {The present study focuses on elucidating the effects of ethylene oxide-based polymers on cellulosic ethanol production from green coconut fiber (GCF). Data on ethanol production, cell viability, adsorption of cellulases, and enzymatic digestibility were collected from experiments with high concentrations of PEG 4000 and poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) 5800 (EOPO 5800). PEG 4000 and EOPO 5800 favored cell viability and ethanol production in simulated experiments with S. cerevisiae CAT-1 and Kluyveromyces marxianus. Enzymatic hydrolysis under 175 g L-1 PEG 4000 increased the sugar release using untreated GCF (702.0%), hydrothermally-pretreated GCF (63.2%), and acid-pretreated GCF (40.1%). However, there were no benefits to using SigmaCell cellulose as the substrate. EOPO 5800 at 100 g L-1 had positive effects on cellulolytic activity preservation and on the sugar release during enzymatic hydrolysis. Both polymers provided higher ethanol titers for simultaneous saccharification and fermentation (SSF) of pretreated GCF compared to polymer-free experiments. A high ethanol yield (89.8%) was reached by the SSF of untreated GCF using 175 g L-1 PEG 4000. This result implies that high polymer concentrations may enable the cellulosic ethanol scheme without the need for pretreatment. (C) 2020 Elsevier Ltd. All rights reserved.},
	keywords = {Ethanol; BIOMASS; PEG; ONE-POT; detoxification},
	year = {2021},
	eissn = {1879-0682},
	pages = {1536-1547},
	orcid-numbers = {de Araujo Padilha, Carlos Eduardo/0000-0002-9532-3026}
}

@article{MTMT:32391975,
	title = {In-situ detoxification strategies to boost bioalcohol production from lignocellulosic biomass},
	url = {https://m2.mtmt.hu/api/publication/32391975},
	author = {Nogueira, Cleitiane da Costa and Padilha, Carlos Eduardo de Araujo and Dantas, Julia Maria de Medeiros and de Medeiros, Fabio Gonsalves Macedo and Guilherme, Alexandre de Araujo and Souza, Domingos Fabiano de Santana and dos Santos, Everaldo Silvino},
	doi = {10.1016/j.renene.2021.09.012},
	journal-iso = {RENEW ENERGY},
	journal = {RENEWABLE ENERGY},
	volume = {180},
	unique-id = {32391975},
	issn = {0960-1481},
	abstract = {The bioalcohol production (ethanol and butanol) from lignocellulosic biomass has some technoeconomic limitations nowadays. Detoxification technologies can efficiently remove fermentation inhibitors (such as furan aldehydes, aliphatic acids, and phenolic compounds) to increase the bioalcohol titers. However, the addition of more steps before fermentation increases the complexity of the production scheme and reduces the profit margin. Process intensification concepts have been disseminated as a critical factor for the success of biotechnological processes, including bioalcohol production. Thus, this review discusses the main inhibitors from lignocellulosic biomass and the in-situ technologies used to minimize the yeast inhibition in hydrolysates and slurries. In-situ detoxification strategies involve different fundamentals, but they have the unanimity of allowing fermentation to be carried out concurrently. They are usually based on the establishment of a microbial consortium as well as the addition of reducing agents, polymers, solvents and adsorbents. Also, in-situ product recovery methods have been developed to minimize energy consumption in downstream processing. (c) 2021 Elsevier Ltd. All rights reserved.},
	keywords = {INHIBITOR; Ethanol; FERMENTATION; butanol; hydrolysate},
	year = {2021},
	eissn = {1879-0682},
	pages = {914-936}
}

@article{MTMT:32323123,
	title = {Strategies for the Cellulosic Ethanol Production by Using High-Concentration Poly(ethylene glycol) in the Pretreatment, Enzymatic Hydrolysis, and Fermentation Steps},
	url = {https://m2.mtmt.hu/api/publication/32323123},
	author = {Nogueira, Cleitiane da Costa and Padilha, Carlos Eduardo de Araujo and Gilherme, Alexande de Araujo and de Souza, Domingos Fabiano Santana and de Oliveira, Jackson Araujo and dos Santos, Everaldo Silvino},
	doi = {10.1007/s12155-021-10306-z},
	journal-iso = {BIOENERG RES},
	journal = {BIOENERGY RESEARCH},
	unique-id = {32323123},
	issn = {1939-1234},
	abstract = {The addition of poly(ethylene glycol) (PEG) in the pretreatment and enzymatic hydrolysis steps has become attractive to increase second-generation ethanol titers. Recent studies have shown the benefits of using high-concentration PEG on the yeast vitality and enzymatic digestibility of untreated biomass. Thus, this study investigated the ideal concentration of PEG in the second-generation ethanol production and the best step to add it. In the acid pretreatment, PEG 4000 favored the delignification (59.2%) and reduced the non-productive adsorption of cellulases onto corn cob, but its use was not significant on the sodium carbonate-based pretreatment. Changes in PEG dosage and PEG molecular weight strongly affected the performance of enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF). PEG 4000 (150 g/L) increased the production of reducing sugars by up to 30.1% and the ethanol production via SSF by up to 44.7% from untreated corn cob. In turn, PEG slightly affected the enzymatic digestibility and fermentability of the pretreated corn cob. One-pot ethanol production from corn cob was performed using PEG as an additive. The addition of 150 g/L PEG 1500 and PEG 4000 increased the ethanol yield values (> 52%) in the one-pot processes; however, the ethanol concentration was lower than in the experiments with the pretreated corn cob. SSF experiments with high solid loadings were tested using PEG 1500. Under these conditions, PEG 1500 slightly increased ethanol production, from 53.4 to 55.7 g/L ethanol (equivalent to 92.8% ethanol yield) using 20% (w/v) acid-pretreated corn cob.},
	keywords = {Ethanol; PRETREATMENT; enzymatic hydrolysis; SURFACTANTS; In situ detoxification},
	year = {2021},
	eissn = {1939-1242}
}

@article{MTMT:32391981,
	title = {Production and Application of Lignin-Based Chemicals and Materials in the Cellulosic Ethanol Production: An Overview on Lignin Closed-Loop Biorefinery Approaches},
	url = {https://m2.mtmt.hu/api/publication/32391981},
	author = {Padilha, Carlos Eduardo de Araujo and Nogueira, Cleitiane da Costa and Alencar, Barbara Ribeiro Alves and de Abreu, Ithalo Barbosa Silva and Dutra, Emmanuel Damilano and Ruiz, Juan Alberto Chavez and Souza, Domingos Fabiano de Santana and dos Santos, Everaldo Silvino},
	doi = {10.1007/s12649-021-01455-5},
	journal-iso = {WASTE BIOMASS VALORI},
	journal = {WASTE AND BIOMASS VALORIZATION},
	volume = {12},
	unique-id = {32391981},
	issn = {1877-2641},
	abstract = {Lignocellulosic biomass is the most abundant biological resource on the planet and has been extensively researched to produce cellulosic ethanol. However, there is a consensus that the presence of lignin hinders the biomass conversion. Lignin is often considered a villain in cellulosic ethanol production studies due to its adverse effects on cellulases and yeasts. Despite this, recent studies indicate that lignins can be transformed into useful inputs to produce cellulosic ethanol. These approaches aim to establish closed-loop biorefineries to improve economic metrics and reduce the environmental impact due to the substitution of products based on fossil sources. The present review addresses the successful cases in transforming lignin into chemicals and materials to increase cellulosic ethanol titers. A contextualization was first carried out, considering aspects of biomass characteristics and lignin valorization. The impact of lignin-based chemicals and materials in the pretreatment, detoxification, and enzymatic hydrolysis steps was discussed in detail. Economic aspects and future perspectives were also included in this review. These reports open a new point of view on lignin valorization and its integration with the cellulosic ethanol production chain.[GRAPHICS].},
	keywords = {enzymatic hydrolysis; ionic liquid; surfactant; ACTIVATED CARBON; Valorization},
	year = {2021},
	eissn = {1877-265X},
	pages = {6309-6337},
	orcid-numbers = {Alencar, Barbara Ribeiro Alves/0000-0002-5733-2773}
}

@article{MTMT:32391976,
	title = {Optimization of Culture Conditions for Cellulase Production in Acanthophysium Sp. KMF001 Using Response Surface Methodology},
	url = {https://m2.mtmt.hu/api/publication/32391976},
	author = {Park, Sohyun and Yoon, Sae-Min and Kim, Yeong-suk and Kim, Young-Kyoon and Yun, Jeonghee},
	doi = {10.15376/biores.16.2.3520-3542},
	journal-iso = {BIORESOURCES},
	journal = {BIORESOURCES},
	volume = {16},
	unique-id = {32391976},
	issn = {1930-2126},
	abstract = {There are differences in the extracellular enzymes produced from species of wood-rotting fungi and their activity due to variation in nutritional conditions such as carbon sources and nitrogen sources, as well as environmental conditions such as incubation temperature and pH. In this study, to determine the methods to promote the secretion of especially cellulase with high activity among the extracellular enzymes of wood-damaging fungi, the optimal nutrient sources and incubation conditions for the production of cellulase with high activity were investigated using response surface methodology based on a broth culture from Acanthophysium sp. KMF001, a novel strain of wood-damaging fungus. The nutrient sources that were optimal for the cellulase production with high activity from Acanthophysium sp. KMF001 were cellulose as a carbon source and tryptone:yeast extract (7:3) as a nitrogen source. The optimal incubation conditions were a temperature of 30 degrees C and a pH of 6. The optimal concentrations of carbon and nitrogen sources were cellulose at 31.1 g.L-1 and tryptone:yeast extract (7:3) at 15 g.L-1, with pH of 5.9.},
	keywords = {nutrient sources; Cellulose activity; Novel wood-damaging fungus; Optimal incubation},
	year = {2021},
	eissn = {1930-2126},
	pages = {3520-3542}
}

@article{MTMT:32323400,
	title = {Optimizing the Saccharification of Pretreated Wood Biomass using Crude Enzyme from Acanthophysium Sp. KMF001},
	url = {https://m2.mtmt.hu/api/publication/32323400},
	author = {Park, Sohyun and Yang, Jae-Kyung and Kim, Young-Kyoon and Yun, Jeonghee and Kim, Yeong-suk},
	doi = {10.15376/biores.16.1.1207-1229},
	journal-iso = {BIORESOURCES},
	journal = {BIORESOURCES},
	volume = {16},
	unique-id = {32323400},
	issn = {1930-2126},
	abstract = {This study was conducted on crude enzyme from the novel strain Acanthophysium sp. KMF001 using a mediator (surfactant). The surfactant was applied to the steam-exploded pretreated domestic wood biomass, and response surface methodology (RSM) was conducted to determine the optimum conditions for saccharification using the optimum substrate concentration, enzyme concentration, and surfactant concentration. Steam-explosion of Korean oak (25 kgf/cm(2)) for 7 min showed a maximum-predicted saccharification of approximately 99.9% at 7.0% substrate concentration, 37.5 FPU (filter paper units) enzyme concentration, and 475.8 mg/g-glucan surfactant (polysorbate 80) concentration. Steam-explosion of red pine (25 kgf/cm(2)) for 7 min revealed a maximum prediction saccharification rate of approximately 58.7% at 6.5% substrate concentration, 36.3 FPU enzyme concentration, and 330.3 mg/g-glucan surfactant (polysorbate 80) concentration. The extents of saccharification of Korean oak (99.9%) and pine (58.7%) demonstrated the high applicability of the crude enzyme from Acanthophysium sp. KMF001.},
	keywords = {Optimization; Cellulase; SACCHARIFICATION; stream-explosion; Acanthophysium sp. KMF001},
	year = {2021},
	eissn = {1930-2126},
	pages = {1207-1229}
}

@article{MTMT:31844457,
	title = {Modeling and optimization of polyethylene glycol (PEG) addition for cost-efficient enzymatic hydrolysis of lignocellulose},
	url = {https://m2.mtmt.hu/api/publication/31844457},
	author = {Pihlajaniemi, V. and Kallioinen, A. and Sipponen, M.H. and Nyyssölä, A.},
	doi = {10.1016/j.bej.2020.107894},
	journal-iso = {BIOCHEM ENG J},
	journal = {BIOCHEMICAL ENGINEERING JOURNAL},
	volume = {167},
	unique-id = {31844457},
	issn = {1369-703X},
	year = {2021},
	eissn = {1873-295X}
}

@article{MTMT:33003574,
	title = {Inhibition of LPMOs by Fermented Persimmon Juice},
	url = {https://m2.mtmt.hu/api/publication/33003574},
	author = {Tokin, Radina and Ipsen, Johan Orskov and Poojary, Mahesha M. and Jensen, Poul Erik and Olsson, Lisbeth and Johansen, Katja Salomon},
	doi = {10.3390/biom11121890},
	journal-iso = {BIOMOLECULES},
	journal = {BIOMOLECULES},
	volume = {11},
	unique-id = {33003574},
	issn = {2218-273X},
	abstract = {Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes. The inhibitory effect of Kakishibu on lytic polysaccharide monooxygenases (LPMOs) and on a cocktail of cellulose hydrolases was studied, using three different cellulosic substrates. Dose dependent inhibition of LPMO activity by a commercial Kakishibu product was assessed for the well-characterized LPMO from Thermoascus aurantiacus TaAA9A, and the inhibitory effect was confirmed on five additional microbial LPMOs. The model tannin compound, tannic acid exhibited a similar inhibitory effect on TaAA9A as Kakishibu. It was further shown that both polyethylene glycol and tannase can alleviate the inhibitory effect of Kakishibu and tannic acid, indicating a likely mechanism of inhibition caused by unspecific tannin-protein interactions.},
	keywords = {INHIBITION; PEG; Tannins; Cellulase; LPMO; Kakishibu},
	year = {2021},
	eissn = {2218-273X},
	orcid-numbers = {Tokin, Radina/0000-0002-8353-1098; Jensen, Poul Erik/0000-0001-6524-7723}
}

@article{MTMT:32323119,
	title = {Screening, cloning, enzymatic properties of a novel thermostable cellulase enzyme, and its potential application on water hyacinth utilization},
	url = {https://m2.mtmt.hu/api/publication/32323119},
	author = {Zhao, Xiaoshen and Liu, Liyang and Deng, Zujun and Liu, Shan and Yun, Jeonyun and Xiao, Xiong and Li, He},
	doi = {10.1007/s10123-021-00170-4},
	journal-iso = {INT MICROBIOL},
	journal = {INTERNATIONAL MICROBIOLOGY},
	volume = {24},
	unique-id = {32323119},
	issn = {1139-6709},
	abstract = {Cellulose is the cheapest, natural, renewable organic substance that is used as a carbon source in various fields. Water hyacinth, an aquatic plant rich in cellulose, is often used as a raw material in fuel production. However, natural cellulase can be hardly used in industrial production on account of its low thermal stability and activity. In this study, a metagenomic library was constructed. Then, a new cellulase gene, cel1029, was screened by Congo red staining and expressed in the prokaryotic system. Enzymatic properties of Cel1029 were explored, including optimum temperature and pH, thermal and pH stability, and tolerance against organic solvents, metal ions, and salt solutions. Finally, its ability of degrading water hyacinth was identified and evaluated. Cel1029 displayed high homology with endoglucanase in the glycoside hydrolase family 5 (GH5) and had high stability across a broad temperature range. More than 86% of its enzymatic activities were retained between 4 and 60 degrees C after 24 h of incubation. Single-factor analysis and orthogonal design were further conducted to determine the optimal conditions for the highest reducing sugar yield of water hyacinth. Interestingly, Cel1029 efficiently transformed water hyacinth with a reducing sugar yield of 430.39 mg/g in 22 h. These findings may open the door for significant industrial applications of a novel GH5 cellulase (NCBI Reference Sequence: MK051001, Cel1029) and help identify more efficient methods to degrade cellulose-rich plants.},
	keywords = {Biotransformation; thermostability; Cellulase; Metagenomic library; Water Hyacinth},
	year = {2021},
	eissn = {1618-1905},
	pages = {337-349}
}

@article{MTMT:31616985,
	title = {Charge-oriented strategies of tunable substrate affinity based on cellulase and biomass for improving in situ saccharification: A review},
	url = {https://m2.mtmt.hu/api/publication/31616985},
	author = {Zhou, Zheng and Ju, Xin and Chen, Jiajia and Wang, Rong and Zhong, Yuqing and Li, Liangzhi},
	doi = {10.1016/j.biortech.2020.124159},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {319},
	unique-id = {31616985},
	issn = {0960-8524},
	year = {2021},
	eissn = {1873-2976}
}

@article{MTMT:31702010,
	title = {Performance targets defined by retro-techno-economic analysis for the use of soybean protein as saccharification additive in an integrated biorefinery},
	url = {https://m2.mtmt.hu/api/publication/31702010},
	author = {Brondi, Mariana G. and Elias, Andrew M. and Furlan, Felipe F. and Giordano, Roberto C. and Farinas, Cristiane S.},
	doi = {10.1038/s41598-020-64316-6},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {10},
	unique-id = {31702010},
	issn = {2045-2322},
	abstract = {The use of additives in the enzymatic saccharification of lignocellulosic biomass can have positive effects, decreasing the unproductive adsorption of cellulases on lignin and reducing the loss of enzyme activity. Soybean protein stands out as a potential lignin-blocking additive, but the economic impact of its use has not previously been investigated. Here, a systematic evaluation was performed of the process conditions, together with a techno-economic analysis, for the use of soybean protein in the saccharification of hydrothermally pretreated sugarcane bagasse in the context of an integrated 1G-2G ethanol biorefinery. Statistical experimental design methodology was firstly applied as a tool to select the process variable solids loading at 15% (w/w) and soybean protein concentration at 12% (w/w), followed by determination of enzyme dosage at 10 FPU/g and hydrolysis time of 24h. The saccharification of sugarcane bagasse under these conditions enabled an increase of 26% in the amount of glucose released, compared to the control without additive. The retro-techno-economic analysis (RTEA) technique showed that to make the biorefinery economically feasible, some performance targets should be reached experimentally such as increasing biomass conversion to ideally 80% and reducing enzyme loading to 5.6 FPU/g in the presence of low-cost soybean protein.},
	year = {2020},
	eissn = {2045-2322}
}

@article{MTMT:31448517,
	title = {Preparation of high molecular weight pH-responsive lignin-polyethylene glycol (L-PEG) and its application in enzymatic saccharification of lignocelluloses},
	url = {https://m2.mtmt.hu/api/publication/31448517},
	author = {Cai, Cheng and Bao, Yu and Jin, Yu and Li, Feiyun and Pang, Yuxia and Lou, Hongming and Qian, Yong and Qiu, Xueqing},
	doi = {10.1007/s10570-019-02800-7},
	journal-iso = {CELLULOSE},
	journal = {CELLULOSE},
	volume = {27},
	unique-id = {31448517},
	issn = {0969-0239},
	abstract = {Highly recyclable pH-responsive lignin-polyethylene glycol (L-PEG) was synthesized to achieve enhanced lignocellulosic hydrolysis and recycling cellulase. The performance of L-PEG could be easily regulated by adjusting the molecular weight and the amount of PEG. The large molecular weight facilitated L-PEG to reduce the invalid adsorption of cellulase on lignin during hydrolysis and enhance its flocculation effect at around pH 3.0. L-PEG(1000-40) obtained by adding 40 wt% (based on lignin) PEG1000 could effectively enhance the enzymatic hydrolysis of lignocelluloses and recover most of cellulase after hydrolysis through simply adjusting the pH of hydrolysate. During eucalyptus hydrolysis, using L-PEG(1000-40) to recycle cellulase could not only save 40% cellulase, but also increase the glucose yield by 121%. Due to the low synthesis cost of L-PEG and the simple and convenient recovery operation, this new method is beneficial to the improvement of lignocellulosic saccharification process and the high-value utilization of lignin. Graphic abstract},
	keywords = {Cellulase; lignocellulose; RECYCLE; pH-responsive; Recyclable; Lignin-PEG},
	year = {2020},
	eissn = {1572-882X},
	pages = {755-767}
}

@article{MTMT:31448515,
	title = {Real-Time QCM-D Monitoring of the Adsorption-Desorption of Expansin on Lignin},
	url = {https://m2.mtmt.hu/api/publication/31448515},
	author = {Cui, Mei and Duan, Yuhao and Ma, Yuanyuan and Al-Shwafy, Khaled W. A. and Liu, Yudong and Zhao, Xudong and Huang, Renliang and Qi, Wei and He, Zhonghe and Su, Rongxin},
	doi = {10.1021/acs.langmuir.0c00104},
	journal-iso = {LANGMUIR},
	journal = {LANGMUIR},
	volume = {36},
	unique-id = {31448515},
	issn = {0743-7463},
	abstract = {Expansin has nonhydrolytic disruptive activity and synergistically acts with cellulases to enhance the hydrolysis of cellulose. The adsorption-desorption of expansin on noncellulosic lignin can greatly affect the action of expansin on lignocellulose. In this study, three lignins with different sources (kraft lignin (KL), sodium lignin sulfonate (SLS), and enzymatic hydrolysis lignin (EHL)) were selected as the substrates. The real-time adsorption-desorption of Bacillus subtilis expansin (BsEXLX1) on lignins was monitored using quartz crystal microgravimetry with dissipation (QCM-D). The effects of temperature and Tween 80 on the adsorption-desorption behaviors were also investigated. The results show that BsEXLX1 exhibited high binding ability on lignin and achieved maximum adsorption of 283.2, 273.8, and 266.9 ng cm(-2) at 25 degrees C on KL, SLS, and EHL, respectively. The maximum adsorption decreased to 148.2-192.8 ng cm(-2) when the temperature increased from 25 to 45 degrees C. Moreover, Tween 80 competitively bound to lignin and significantly prevented expansin adsorption. After irreversible adsorption of Tween 80, the maximum adsorption of BsEXLX1 greatly decreased to 33.3, 37.2, and 10.3 ng cm(-2) at 25 degrees C on la, SLS, and EHL, respectively. Finally, a kinetic model was developed to analyze the adsorption- desorption process of BsEXLX1. BsEXLX1 has a higher adsorption rate constant (k(A)) and a lower desorption rate constant (k(D)) on KL than on SLS and EHL. The findings of this study provide useful insights into the adsorption-desorption of expansin on lignin.},
	year = {2020},
	eissn = {1520-5827},
	pages = {4503-4510}
}

@article{MTMT:31702007,
	title = {Slow degrading poly(glycerol sebacate) derivatives improve vascular graft remodeling in a rat carotid artery interposition model},
	url = {https://m2.mtmt.hu/api/publication/31702007},
	author = {Fu, Jiayin and Ding, Xiaochu and Stowell, Chelsea E. T. and Wu, Yen-Lin and Wang, Yadong},
	doi = {10.1016/j.biomaterials.2020.120251},
	journal-iso = {BIOMATERIALS},
	journal = {BIOMATERIALS},
	volume = {257},
	unique-id = {31702007},
	issn = {0142-9612},
	abstract = {Porous synthetic grafts made of poly (glycerol sebacate) (PGS) can transform into autologous vascular conduits in vivo upon degradation of PGS. A long-held doctrine in tissue engineering is the necessity to match degradation of the scaffolds to tissue regeneration. Here, we tested the impact of degradation of PGS and its derivative in an interposition model of rat common carotid artery (CCA). Previous work indicates a complete degradation of PGS within approximately 2 weeks, likely at the fast end of the spectrum. Thus, the derivation of PGS focuses on delay degradation by conjugating the free hydroxy groups in PGS with a long chain carboxylic acid: palmitic acid, one of the most common lipid components. We evaluated two of the resultant palmitate-PGS (PPGS) in this study: one containing 9% palmitate (9-PPGS) and the other 16% palmitate (16-PPGS). 16-PPGS grafts had the highest patency. Ultrasound imaging showed that the lumens of 16-PPGS grafts were similar to CCA and smaller than 9-PPGS and PGS grafts 12 weeks post-operation. Immunohistological and histological examination showed an endothelialized lumens in all three types of grafts within 4 weeks. Inflammatory responses to 16-PPGS grafts were limited to the adventitial space in contrast to a more diffusive infiltration in 9-PPGS and PGS grafts in week 4. Examination of calponin(+) and alpha SMA(+) cells revealed that 16-PPGS grafts remodeled into a distinctive bilayered wall, while the walls of 9-PPGS grafts and PGS grafts only had one thick layer of smooth muscle-like cells. Correspondingly, the expression of collagen III and elastin displayed an identical layered structure in the remodeled 16-PPGS grafts, in contrast to a more spread distribution in 9-PPGS and PGS grafts. All the three types of grafts exhibited the same collagen content and burst pressure after 12 weeks of host remodeling. However, the compliance and elastin content of 16-PPGS grafts in week 12 were closest to those of CCA. Overall, placing the degradation of PGS derived elastomer to a window of 4-12 weeks results in vascular conduits closer to arteries in a rat carotid artery interposition model over a 12-week observation period.},
	keywords = {DEGRADATION; Tissue Engineering; ARTERY; vascular graft; Poly(glycerol sebacate)},
	year = {2020},
	eissn = {1878-5905}
}

@article{MTMT:31448520,
	title = {Lignocellulosic conversion into value-added products: A review},
	url = {https://m2.mtmt.hu/api/publication/31448520},
	author = {Haldar, Dibyajyoti and Purkait, Mihir Kumar},
	doi = {10.1016/j.procbio.2019.10.001},
	journal-iso = {PROCESS BIOCHEM},
	journal = {PROCESS BIOCHEMISTRY},
	volume = {89},
	unique-id = {31448520},
	issn = {1359-5113},
	abstract = {In the present context of energy crisis, exploration of lignocellulosic biomass has emerged as potential substitute to maintain environmental sustainability. However, the conversion of biomass into value-added products still faces challenges to find a suitable unit operation. The stubborn dependency on the cost intensive enzymatic system, limits an effective saccharification to hydrolyze the biomass. India has been one of the top most agriculturally enriched countries with broad scope of utilizing waste residue that remains as an unused biomass at harvested locations. Hence, in the present script, an overview on the latest R&D initiatives taken by Government of India are briefed to highlight the projects based on biofuels and in addition, global scenario of biofuel production is comprehensively discussed. Further, critical analysis on the advancement of different pretreatment operations are highlighted through latest inventions. Thereafter, biochemistry of cellulase enzyme with essential factors were explored to understand the mechanistic interactions involved during saccharification of biomass. An insight on the latest accomplishments of various fermentation process provides an in depth understanding of metabolic engineering based on the genetic studies of fermentative microorganisms. Finally, the article is concluded with brief discussions on fate of the derivatives obtained from macromolecules such as cellulose and lignin.},
	keywords = {HYDROLYSIS; FERMENTATION; lignocellulose; BIOMASS CONVERSION; Value-added products},
	year = {2020},
	eissn = {1873-3298},
	pages = {110-133}
}

@article{MTMT:31700037,
	title = {Experimental investigation of the adsorption and desorption of cellulase enzymes on zeolite-beta for enzyme recycling applications},
	url = {https://m2.mtmt.hu/api/publication/31700037},
	author = {Jampana, Surya R. and Jia, Linjing and Ramarao, Bandaru V and Kumar, Deepak},
	doi = {10.1007/s00449-020-02459-6},
	journal-iso = {BIOPROC BIOSYST ENG},
	journal = {BIOPROCESS AND BIOSYSTEMS ENGINEERING},
	unique-id = {31700037},
	issn = {1615-7591},
	abstract = {The recyclability of cellulase enzymes using zeolite and polyethylene glycol (PEG) was investigated. The cellulase enzymes from cellulose hydrolysate suspensions were adsorbed onto zeolite-beta under typical working conditions (pH 5). PEG having a molecular weight of 200 Da and 20 kDa was used as an eluent to desorb the cellulase enzymes from zeolite-beta. Adsorption and desorption profiles of cellulase enzymes were studied by varying pH, PEG concentration, and salt concentration. Maximum binding capacity, q(m)of the zeolite decreased by increasing the pH, or by introducing PEG. At pH 5, the q(m) of the zeolite was determined to be 121 x 10(-3)g/g. About 24%, 51% and 75% of the adsorbed enzyme can be recovered using 1 M NaCl, PEG 200 and PEG 20000, respectively. The specific activity of the recovered enzyme increased by 57% due to the presence of residual PEG.},
	keywords = {PEG; ZEOLITE-BETA; Enzyme adsorption; Enzyme desorption; Enzyme recycle},
	year = {2020},
	eissn = {1615-7605}
}

@article{MTMT:31448522,
	title = {Boost Effect of Water-Soluble Polymers on Enzymatic Hydrolysis of Lignocellulosic Biomass},
	url = {https://m2.mtmt.hu/api/publication/31448522},
	author = {Pallapolu, Venkata R. and Shi, Suan and Kang, Li and Kothari, Urvi and Li, Jing},
	doi = {10.1021/acs.iecr.9b05850},
	journal-iso = {IND ENG CHEM RES},
	journal = {INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH},
	volume = {59},
	unique-id = {31448522},
	issn = {0888-5885},
	abstract = {Supplementation of surface-active additives during enzymatic saccharification of biomass helps to increase the activity of cellulase and thus reduces the enzyme dosage in the bioconversion processes. To evaluate the boost effect of additives on enzymatic hydrolysis, five different water-soluble polymers were studied in this work, including poly(vinyl alcohol) (PVA), poly(vinyl pyrrolidone) (PVP), polyethylene glycol) (PEG), cationic polyacrylamide (C-PAM), and polyethyleneimine (PEI). Lignin-containing substrates and lignin-free substrates were used in enzymatic hydrolysis tests. It was found that an addition of 10 mg polymer/g-substrate leads to up to a 25.4% increase in glucan hydrolysis and a 13.2% increase in xylan hydrolysis depending on the type of the substrate and polymer. Glucan digestibility achieved from low enzyme loading of 5 FPU/g-glucan with the polymer is comparable to that from 15 FPU/g-glucan enzyme loading. Reducing the amount of the enzyme required could make biomass conversion more cost-effective. Significantly higher yields were obtained with nonionic polymers (PVP, PVA, and PEG) compared with ionic polymers (PEI and C-PAM) for all substrates.},
	year = {2020},
	eissn = {1520-5045},
	pages = {5186-5193}
}

@article{MTMT:31448518,
	title = {Distinct mechanisms of enzymatic saccharification and bioethanol conversion enhancement by three surfactants under steam explosion and mild chemical pretreatments in bioenergy Miscanthus},
	url = {https://m2.mtmt.hu/api/publication/31448518},
	author = {Sun, Dan and Yang, Qiaomei and Wang, Yanting and Gao, Hairong and He, Mingxiong and Lin, Xinchun and Lu, Jun and Wang, Youmei and Kang, Heng and Alam, Aftab and Tu, Yuanyuan and Xia, Tao and Peng, Liangcai},
	doi = {10.1016/j.indcrop.2020.112559},
	journal-iso = {IND CROP PROD},
	journal = {INDUSTRIAL CROPS AND PRODUCTS},
	volume = {153},
	unique-id = {31448518},
	issn = {0926-6690},
	abstract = {Miscanthus is a leading bioenergy crop that represents an enormous lignocellulose resource for biofuels and bioproducts. However, as lignocellulose recalcitrance leads to financially inviable bioethanol production and the potential of secondary wastes into the environment, it becomes crucial to explore green-like and cost-effective biomass processing technologies. To address these issues, low doses of chemical surfactants have been added to enhance biomass enzymatic hydrolysis and bioethanol conversion, but much remains unknown about the mechanism of enhancement. For first time, in this study, a novel chemical surfactant (1% Silwet L-77) was applied to enhance the enzymatic hydrolysis of raw Miscanthus straw, and 40% cellulose digestion was achieved, which is 1.2- and 4.5-fold higher than that of two well-known surfactants (PEG-4000 and Tween-80), respectively. Using lignocellulose substrates obtained from Miscanthus biomass samples that were pretreated by green-like steam explosion followed by mild chemical (NaOH or H2SO4) pretreatments, supplementation with the three surfactants led to significantly enhanced enzymatic saccharification. The 2% Tween-80 supply resulted in a hexose yield of 99% (% cellulose) from enzymatic hydrolysis, followed by 95% with 0.5% PEG-4000 and 71% with 1% Silwet L-77. Despite the slightly lower hexose yield, Silwet L-77 resulted in consistently higher sugar-ethanol conversion rates in all lignocellulose substrates examined. Furthermore, based on the enzyme profiling of mixed cellulase adsorption on lignocellulose and the chemical analysis of wall polymer features and lignocellulose accessibility, this study proposed multiple hypothetical models to interpret the distinct enhancement roles of three surfactants in the enzymatic hydrolyses of diverse lignocellulose substrates. These models also provide a powerful strategy for low-cost bioethanol production with the potential for high-value bioproducts by using desirable surfactants in Miscanthus and other bioenergy crops.},
	keywords = {surfactant; Miscanthus; Steam explosion; Enzyme adsorption; Biomass saccharification; Bioethanol conversion},
	year = {2020},
	eissn = {1872-633X}
}

@article{MTMT:31448525,
	title = {Humic acid-assisted autohydrolysis of waste wheat straw to sustainably improve enzymatic hydrolysis},
	url = {https://m2.mtmt.hu/api/publication/31448525},
	author = {Tang, Wei and Wu, Xinxing and Huang, Chen and Huang, Caoxing and Lai, Chenhuan and Yong, Qiang},
	doi = {10.1016/j.biortech.2020.123103},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {306},
	unique-id = {31448525},
	issn = {0960-8524},
	abstract = {Humic acid (HA), considered the main component of organic matter in the ash of waste wheat straw (WWS), has the potential to improve autohydrolysis through its function as a surfactant. In this work, a pre-washed WWS (PWWS) was subjected to autohydrolysis with addition of HA to explore whether its surfactant properties can provide benefit to biorefinery operations. Acquired results showed that HA acted as delignification agent likely due to its surfactant properties. Delignification was more than doubled at the maximum HA dosage (30 g/L) relative to the control, which allowed for enzymatic hydrolysis efficiency to also increase from 64.9% to 81.8%. The pretreated materials were further subjected to analysis structure characterization. The results showed that HA effectively reduced the surface lignin area of PWWS, lowering non-specific adsorption of lignin to enzymes. The autohydrolysis with HA was an effective technique to improve the subsequent cellulose enzymatic digestion by enhancing the delignification.},
	keywords = {DELIGNIFICATION; surfactant; humic acid; Prewashed waste wheat straw},
	year = {2020},
	eissn = {1873-2976}
}

@article{MTMT:31702009,
	title = {Adsorption and desorption of cellulase on/from enzymatic residual lignin after alkali pretreatment},
	url = {https://m2.mtmt.hu/api/publication/31702009},
	author = {Wang, Jinye and Wang, Jia and Lu, Zhoumin and Zhang, Junhua},
	doi = {10.1016/j.indcrop.2020.112811},
	journal-iso = {IND CROP PROD},
	journal = {INDUSTRIAL CROPS AND PRODUCTS},
	volume = {155},
	unique-id = {31702009},
	issn = {0926-6690},
	abstract = {The knowledge about adsorption/desorption of cellulase on/from residual lignin is essential for efficient cellulose hydrolysis by recycling the cellulase from residual solid fraction. Herein, the impact of alkali pretreatment at different alkali strength on the adsorption/desorption of enzymatic residual lignins (ERLs) with cellulase was investigated. ERLs pretreated at stronger alkali strength had higher molecular weights and negative zeta potentials, but lower hydrophobicity. The inhibition of ERL to the enzymatic cellulose digestion was mitigated by alkali pretreatment, and inhibition degrees decreased from 21.5 % (with un-pretreated ERL) to 18.9 %, 12.9 %, and 10.8 % with the addition of ERLs treated with 0.5 %, 1.0 %, and 1.5 % (w/w) sodium hydroxide aqueous solution, respectively. The affinity of ERLs with cellulase diminished after alkali pretreatment, and the binding strength decreased from 183.0 to 130.7 mL/g with the alkali strength increasing, retaining more cellulase activities in supernatant. Desorption capacity of bound-cellulase from ERL increased after alkali pretreatment, and the recovery increased from 65.3% to 73.1% with the alkali strength increasing. The cellulase released after desorption exhibited hydrolytic activity, and higher glucose yields were obtained by the bound-cellulase on alkali-pretreated ERLs compared with that of un-pretreated ERL. The results provide references for the development of alkali pretreatment and recycling cellulase from residual lignin for efficient lignocellulosics digestion.},
	keywords = {ADSORPTION; DESORPTION; Cellulase; alkali pretreatment; Enzymatic residual lignin},
	year = {2020},
	eissn = {1872-633X}
}

@article{MTMT:31059623,
	title = {Achieving high ethanol yield by co-feeding corncob residues and tea seed cake at high-solids simultaneous saccharification and fermentation},
	url = {https://m2.mtmt.hu/api/publication/31059623},
	author = {Zheng, Tianran and Yu, Hailong and Liu, Shijie and Jiang, Jianxin and Wang, Kun},
	doi = {10.1016/j.renene.2019.06.083},
	journal-iso = {RENEW ENERGY},
	journal = {RENEWABLE ENERGY},
	volume = {145},
	unique-id = {31059623},
	issn = {0960-1481},
	abstract = {The feasibility of high-solids simultaneous saccharification and ethanol production was investigated using unwashed corncob residues (CRs) with tea-seed cake (TSC). Tea saponin of TSC could serve as an accelerant to enhance ethanol production and reduce the dosage of enzymes. The proteins of TSC could be the nitrogen resource for yeast. The effects of substrate concentration (10%, 15%, and 20% (w/w)), enzyme dosage (2.5-15 FPU/g-cellulose), and types of media on ethanol production were evaluated. An ethanol yield of 86.56% of the theoretical maximum could be obtained at a substrate concentration of 15% (w/w) with 10 FPUJg-cellulose. Furthermore, fermentations in different media showed that the surface tension (49.21 mN/m) and contact angle (42.6 degrees) of the fermentation system with TSC were lower than those from the other systems. This study found that adding TSC to the fermentation system was an attractive strategy to achieve high ethanol yield without any pretreatment. Comprehensive utilization of CRs and TSC as feedstocks for ethanol production can reduce the cost of biorefineries with environmental benefits. (C) 2019 Elsevier Ltd. All rights reserved.},
	keywords = {FERMENTATION; High-solids loading; Corncob residues; Tea-seed cake; Simultaneous saccharification and ethanol},
	year = {2020},
	eissn = {1879-0682},
	pages = {858-866}
}

@article{MTMT:30565451,
	title = {Alternative Low-Cost Additives to Improve the Saccharification of Lignocellulosic Biomass},
	url = {https://m2.mtmt.hu/api/publication/30565451},
	author = {Brondi, Mariana G. and Vasconcellos, Vanessa M. and Giordano, Roberto C. and Farinas, Cristiane S.},
	doi = {10.1007/s12010-018-2834-z},
	journal-iso = {APPL BIOCHEM BIOTECH},
	journal = {APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY},
	volume = {187},
	unique-id = {30565451},
	issn = {0273-2289},
	abstract = {A potential strategy to mitigate problems related to unproductive adsorption of enzymes onto lignin during the saccharification of lignocellulosic biomass is the addition of lignin-blocking agents to the hydrolysis reaction medium. However, there is a clear need to find more cost-effective additives for use in large-scale processes. Here, selected alternative low-cost additives were evaluated in the saccharification of steam-exploded sugarcane bagasse using a commercial enzymatic cocktail. The addition of soybean protein, tryptone, peptone, and maize zein had positive effects on glucose release during the hydrolysis, with gains of up to 36% when 8% (w/w) soybean protein was used. These improvements were superior to those obtained using bovine serum albumin (BSA), a much more expensive protein that has been widely reported for such an application. Moreover, addition of soybean protein led to a saving of 48h in the hydrolysis, corresponding to a 66% decrease in the reactor operation time required. In order to achieve the same hydrolysis yield without the soybean additive, the enzyme loading would need to be increased by 50%. FTIR spectroscopy and nitrogen elemental analysis revealed that the additives probably acted to reduce unproductive binding of cellulolytic enzymes onto the lignin portion of the sugarcane bagasse.},
	keywords = {ADSORPTION; LIGNIN; ADDITIVES; enzymatic hydrolysis; LIGNOCELLULOSIC BIOMASS; Sugarcane bagasse},
	year = {2019},
	eissn = {1559-0291},
	pages = {461-473}
}

@article{MTMT:31021093,
	title = {Dissecting the effect of polyethylene glycol on the enzymatic hydrolysis of diverse lignocellulose},
	url = {https://m2.mtmt.hu/api/publication/31021093},
	author = {Li, Huanan and Wang, Chaoying and Xiao, Wenjing and Yang, Yuxian and Hu, Pan and Dai, Yujun and Jiang, Zhengbing},
	doi = {10.1016/j.ijbiomac.2019.03.131},
	journal-iso = {INT J BIOL MACROMOL},
	journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
	volume = {131},
	unique-id = {31021093},
	issn = {0141-8130},
	abstract = {Natural lignocellulose is used as raw material to produce chemicals through biological transformation. The accessibility of cellulase to substrate was also one of the limiting factors of industrial production. Polyethylene glycol (PEG) can be used as additive in enzymatic hydrolysis of lignocellulose. In this study, enzymatic activity on simultaneous or non-simultaneous addition of PEG 4000 was investigated, and the partly delignified rice straw, the rice straw and filter paper were used as substrates, respectively. Enzyme activity was characterized by reducing sugar concentration in supernatant which was quantified through 3,5-dinitrosalicylic acid (DNS) method. Addition of PEG has been proven to facilitate enzymatic hydrolysis of lignocellulosic materials. Furthermore, PEG had the positive effect on hydrolytic enzyme activity of pure cellulose materials without lignin. Changes in lignocellulose materials have been observed by inverted microscope and Scanning electron microscope (SEM), and no chemical changes were shown by Fourier transform infrared spectroscopy (FTIR). The promotion of PEG on enzymatic hydrolysis of pure cellulose materials may be due to its loose physical structure and similar phenomenon in natural lignin materials. PEG loosens the physical structure of lignocellulose, thus facilitating enzymatic hydrolysis. This may be a new idea to optimize the lignocellulosic enzymatic hydrolysis process. (C) 2019 Elsevier B.V. All rights reserved.},
	keywords = {Cellulase; PEG 4000; lignocellulose},
	year = {2019},
	eissn = {1879-0003},
	pages = {676-681}
}

@article{MTMT:31059627,
	title = {Enhancement of polyhydroxybutyrate (PHB) production by 10-fold from alkaline pretreatment liquor with an oxidative enzyme-mediator-surfactant system under Plackett-Burman and central composite designs},
	url = {https://m2.mtmt.hu/api/publication/31059627},
	author = {Li, Mengxing and Eskridge, Kent and Liu, Enshi and Wilkins, Mark},
	doi = {10.1016/j.biortech.2019.02.045},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {281},
	unique-id = {31059627},
	issn = {0960-8524},
	abstract = {In this study, Plackett-Burman and central composite designs were applied to improve polyhydroxybutyrate (PHB) production from alkaline pretreatment liquor (APL) by Cupriavidus necator DSM 545 using a supplement system consisting of oxidative enzymes (laccase, aryl alcohol oxidase (AAO)), mediators (ABTS, HOBT), DMSO, silica nanoparticle Aerosol R816 and surfactant Tween 80. First, screening experiments under Plackett-Burman design showed R816, ABTS and Tween 80 could significantly enhance PHB production. Additional experiments showed that HOBT and DMSO could be removed, and laccase and AAO were needed to remain in the system. Second, a central composite design was applied to obtain the optimum supplemental levels of R816, ABTS and Tween 80. Under optimum conditions, theoretical maximum PHB production (1.9 g/L) was close to experimental PHB production (2.1 g/L). With the supplement system, a 10-fold increase was achieved compared to PHB production (0.2 g/L) without any supplements.},
	keywords = {Polyhydroxybutyrate; Central composite design; Plackett-Burman design; Alkaline pretreatment liquor (APL); 10-Fold increase},
	year = {2019},
	eissn = {1873-2976},
	pages = {99-106}
}

@article{MTMT:31059624,
	title = {Improved enzymatic hydrolysis of hardwood and cellulase stability by biomass kraft lignin-based polyoxyethylene ether},
	url = {https://m2.mtmt.hu/api/publication/31059624},
	author = {Lin, Xuliang and Yang, Yaosen and Wu, Lei and Wu, Linjun and Xu, Danyuan and Qin, Yanlin},
	doi = {10.1016/j.ijbiomac.2019.06.105},
	journal-iso = {INT J BIOL MACROMOL},
	journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
	volume = {136},
	unique-id = {31059624},
	issn = {0141-8130},
	abstract = {Water-soluble kraft lignin-based polyoxyethylene ether (KL-PEG), synthesized from the black liquor of kraft pulping and PEG, was used to improve the enzymatic hydrolysis efficiency of dilute acid pretreated (DA pretreated) Eucalyptus hardwood and cellulase stability. The physicochemical properties of KL-PEG polymer such as solubility, surface tension, charge and aggregation behavior in the solution were first studied. KL-PEG could enhance the enzymatic hydrolysis of Avicel and DA-pretreated Eucalyptus from 63.6% and 58.3% to 78.5% and 93.8%, respectively. The enzymatic activity of cellulase after the enzymatic hydrolysis of Avicel and DA-pretreated Eucalyptus for 72 h remained approximately 84% and 44% in the presence of KL-PEG polymer. KL-PEG could improve the stability and longevity of the cellulase, facilitate the recovery and save the amount of cellulase. The efficient utilization of the pulping black liquor lignin was of great significance to alleviate the pressure brought by the shortage of petrochemical resources, and build an energy-saving and low-carbon society. (C) 2019 Elsevier B.V. All rights reserved.},
	keywords = {enzymatic hydrolysis; Lignin-based polyoxyethylene ether; Black liquor},
	year = {2019},
	eissn = {1879-0003},
	pages = {540-546}
}

@article{MTMT:31059619,
	title = {Tween 80 enhancing cellulasic activation of hardwood kraft-based dissolving pulp},
	url = {https://m2.mtmt.hu/api/publication/31059619},
	author = {Liu, Shanshan and He, Huili and Fu, Xin and Wang, Yingchao and Wang, Qiang and Yang, Guihua and Chen, Jiachuan and Ni, Yonghao},
	doi = {10.1016/j.indcrop.2019.05.026},
	journal-iso = {IND CROP PROD},
	journal = {INDUSTRIAL CROPS AND PRODUCTS},
	volume = {137},
	unique-id = {31059619},
	issn = {0926-6690},
	abstract = {Cellulase treatment is a promising approach to activate dissolving pulp in terms of viscosity and reactivity. However, the enzyme cost is still too high, which hinders the scale up application. In this study, Tween 80 was introduced into the enzymatic system for improving the cellulase efficiency by improving the enzyme accessibility and its stability. Results show that the cellulose chain scission rate was more than 2 times higher with the addition of Tween 80 (0.1 g/L) in comparison to the control, which is mainly attributed to decreased surface tension (by 28.0% with the addition of 0.1 g/L Tween) and increased relative cellulase activity (by 9% at 48 h and 55 degrees C). As a result, Tween 80 assisted cellulase activation gave a higher cellulasic efficiency in terms of viscosity decrease and Fock reactivity increase. Other pulp properties (alkali solubility, molecular weight distribution) and pulp yield were all favorable, supporting the conclusion that the Tween 80- assisted cellulase treatment process has a great potential to improve the cellulase treatment performance of dissolving pulp.},
	keywords = {Viscosity; REACTIVITY; Cellulase; Tween 80; Dissolving pulp},
	year = {2019},
	eissn = {1872-633X},
	pages = {144-148}
}

@article{MTMT:31058043,
	title = {Improved Cellulosic Ethanol Titres from Highly Lignified Cotton Trash Residues Using Various Batch and Fed-Batch Process Configurations},
	url = {https://m2.mtmt.hu/api/publication/31058043},
	author = {McIntosh, S. and Palmer, J. and Egbuta, M. and Liu, L. and Vancov, Tony},
	doi = {10.1007/s12155-019-10023-8},
	journal-iso = {BIOENERG RES},
	journal = {BIOENERGY RESEARCH},
	volume = {12},
	unique-id = {31058043},
	issn = {1939-1234},
	abstract = {This study investigates a fed-batch simultaneous saccharification fermentation (F-SSF) process to increase ethanol titres from highly lignified (41.6 wt.%) cotton gin trash residue. The optimal initial solid loading, enzyme dose, feed quantities and intervals to maximize substrate feed and subsequent ethanol titres were examined. Under batch SSF conditions, initial extracted cotton gin trash (ECGT) solid loadings were maximised at 19.35 wt.% and attained an ethanol titre of 23.3 g/l with a corresponding yield of 53.7%. Operating under optimised F-SSF mode, fermentations were initiated with 16.13 wt% EGCT solids followed by fresh ECGT feeds of 16.13 wt% and 12.9 wt.% at 12-h intervals. Cellulase levels were maintained at 44 FPU/g glucan throughout the fermentations. The final ethanol titre of 41 .4 g/l with a corresponding conversion rate of 70.1% was achieved after 72 h. Comparable ethanol yields of 40 g/l with 67.8% conversion were realized with lower cellulase dosing (25 FPU g/glucan) but only after extending the fermentation by 24 h.},
	keywords = {High solid loading; Cotton gin trash; Fed-batch SSF; Saccharomyces cerevisiae strain Fali (R)},
	year = {2019},
	eissn = {1939-1242},
	pages = {1021-1032}
}

@article{MTMT:31059630,
	title = {Enhancing Lignocellulosic Biomass Hydrolysis by Hydrothermal Pretreatment, Extraction of Surface Lignin, Wet Milling and Production of Cellulolytic Enzymes},
	url = {https://m2.mtmt.hu/api/publication/31059630},
	author = {Nitsos, Christos K. and Lazaridis, Polykarpos A. and Mach-Aigner, Astrid and Matis, Kostas A. and Triantafyllidis, Konstantinos S.},
	doi = {10.1002/cssc.201802597},
	journal-iso = {CHEMSUSCHEM},
	journal = {CHEMSUSCHEM},
	volume = {12},
	unique-id = {31059630},
	issn = {1864-5631},
	abstract = {Acetone and ethanol extraction of lignin deposits from the surface of hydrothermally (liquid hot water) pretreated beech wood biomass alleviates the lignin inhibitory effects during enzymatic hydrolysis of cellulose to glucose and boosts the enzymatic digestibility to high values (approximate to 70 %). Characterization of the extracted lignins (FTIR, pyrolysis/GC-MS, differential thermogravimetry, gel permeation chromatography) indicated high purity, low molecular weight, and features that suggest that it consists mainly of fragments of the native wood lignin partially depolymerized and recondensed on the biomass surface during the hydrothermal pretreatment. The pyrolysis products of the extracted surface lignins suggest their high potential as a feedstock for the production of high added value phenolic compounds. When the enzymatic hydrolysis of the pretreated and extracted biomass solids was assisted by mild wet milling, near complete cellulose digestibility (>= 95 %) could be achieved. In the context of the biorefinery and whole-biomass valorization concept, it was also shown that the hydrothermally (hemicellulose-deficient) pretreated and delignified biomass solids could be also successfully used for the production of crude cellulase from Trichoderma reesei cultures, providing a simple and low-cost method for the complementary production of cellulases by utilizing fractions of the integrated hydrolysis process.},
	keywords = {enzymatic hydrolysis; Cellulase; Wet milling; Hydrothermal pretreatment; surface lignin},
	year = {2019},
	eissn = {1864-564X},
	pages = {1179-1195}
}

@article{MTMT:31059633,
	title = {Nanoemulsion formation by the phase inversion temperature method using polyoxypropylene surfactants},
	url = {https://m2.mtmt.hu/api/publication/31059633},
	author = {Ren, Gaihuan and Sun, Zhicheng and Wang, Zengzi and Zheng, Xiaoyang and Xu, Zhenghe and Sun, Dejun},
	doi = {10.1016/j.jcis.2019.01.018},
	journal-iso = {J COLLOID INTERF SCI},
	journal = {JOURNAL OF COLLOID AND INTERFACE SCIENCE},
	volume = {540},
	unique-id = {31059633},
	issn = {0021-9797},
	abstract = {Hypothesis: Surfactants with temperature-sensitive polyoxyethylene (POE) chains are widely used to prepare nanoemulsions by the phase inversion temperature (PIT) method. It is therefore anticipated that surfactants with temperature-sensitive polyoxypropylene (POP) chains could also be used to prepare nanoemulsions by the PIT method.Experiment: POP surfactants were synthesized through electrostatic interactions between hydrophilic POP diamines and hydrophobic long-chain fatty acids. The synthesized POP surfactants were used as emulsifiers to prepare n-tetradecane-in-water nanoemulsions by the PIT method. Electrical conductivity measurements were used to determine the PITs of the water/POP surfactant/n-tetradecane systems. The effects of surfactant concentration, NaCI concentration, number of POP units, and degree of unsaturation of hydrocarbon chains on the PIT and the nanoemulsion droplet size were investigated. The droplet size and morphology of the nanoemulsions were characterized by dynamic light scattering and cryogenic transmission electron microscopy, respectively.Findings: Nanoemulsions are formed by the PIT method using POP surfactants when the POP chains are short (similar to 2.5-6.1 POP units). The formation of nanoemulsions with droplet radii of 20-300 nm and spherical morphology occurs because of the temperature-dependent hydration of the short POP chains. (C) 2019 Elsevier Inc. All rights reserved.},
	keywords = {nanoemulsion; Phase inversion temperature; Long-chain fatty acid; Polyoxypropylene surfactant; Polyoxypropylene diamine},
	year = {2019},
	eissn = {1095-7103},
	pages = {177-184},
	orcid-numbers = {Xu, Zhenghe/0000-0001-8118-1920}
}

@article{MTMT:30565450,
	title = {Effect of Metal Ions and Surfactants on the Enzymatic Hydrolysis of Pretreated Lignocellulose},
	url = {https://m2.mtmt.hu/api/publication/30565450},
	author = {Zhou, Yawen and Yang, Jian and Luo, Cong and Yang, Bo and Liu, Changyao and Xu, Baocai},
	doi = {10.15376/biores.14.1.1653-1667},
	journal-iso = {BIORESOURCES},
	journal = {BIORESOURCES},
	volume = {14},
	unique-id = {30565450},
	issn = {1930-2126},
	abstract = {The effect of metal ions and surfactants on the enzymatic hydrolysis of pretreated wheat straw lignocellulose was investigated. Scanning electron microscopy, infrared spectrum analysis, dynamic light scattering, and fluorescence spectra analysis were used to characterize the influence of Fe3+/ polyoxyethylene (20) sorbitan monooleate (Tween 80). The interaction between Fe3+/Tween 80 and enzyme was further investigated by enzyme kinetics and enzyme activity measurements. The best synergistic effect was obtained when the ratio of Fe3+ and Tween 80 was 0.06. The scanning electron microscopy images showed that the Fe3+/Tween 80 combination was associated with high porosity substrates. The infrared spectrum analysis indicated that the components of the substrates depended on additive types. The highest relative enzymatic activity increase rate was obtained with added Fe3+/ Tween 80. The V-max and K-m values of the group with added Fe3+/Tween 80 were much higher than that of the group without additives. With the addition of Fe3+/ Tween 80, the intensity of the fluorescence emission peak decreased and the peak shifted towards a longer wavelength.},
	keywords = {CELLULOSE; enzymatic hydrolysis; metal ions; SURFACTANTS},
	year = {2019},
	eissn = {1930-2126},
	pages = {1653-1667}
}

@article{MTMT:27579933,
	title = {Enhancing enzyme-aided production of fermentable sugars from poplar pulp in the presence of non-ionic surfactants},
	url = {https://m2.mtmt.hu/api/publication/27579933},
	author = {Alhammad, A and Adewale, P and Kuttiraja, M and Christopher, L P},
	doi = {10.1007/s00449-018-1942-z},
	journal-iso = {BIOPROC BIOSYST ENG},
	journal = {BIOPROCESS AND BIOSYSTEMS ENGINEERING},
	volume = {41},
	unique-id = {27579933},
	issn = {1615-7591},
	year = {2018},
	eissn = {1615-7605},
	pages = {1133-1142}
}

@article{MTMT:27325611,
	title = {Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes},
	url = {https://m2.mtmt.hu/api/publication/27325611},
	author = {Djajadi, Demi T and Jensen, Mads M and Oliveira, Marlene and Jensen, Anders and Thygesen, Lisbeth G and Pinelo, Manuel and Glasius, Marianne and Jorgensen, Henning and Meyer, Anne S},
	doi = {10.1186/s13068-018-1085-0},
	journal-iso = {BIOTECHNOL BIOFUELS},
	journal = {BIOTECHNOLOGY FOR BIOFUELS},
	volume = {11},
	unique-id = {27325611},
	issn = {1754-6834},
	year = {2018},
	eissn = {1754-6834}
}

@article{MTMT:30565454,
	title = {Cellulases adsorb reversibly on biomass lignin},
	url = {https://m2.mtmt.hu/api/publication/30565454},
	author = {Djajadi, Demi T. and Pihlajaniemi, Ville and Rahikainen, Jenni and Kruus, Kristiina and Meyer, Anne S.},
	doi = {10.1002/bit.26820},
	journal-iso = {BIOTECHNOL BIOENG},
	journal = {BIOTECHNOLOGY AND BIOENGINEERING},
	volume = {115},
	unique-id = {30565454},
	issn = {0006-3592},
	abstract = {Adsorption of cellulases onto lignin is considered a major factor in retarding enzymatic cellulose degradation of lignocellulosic biomass. However, the adsorption mechanisms and kinetics are not well understood for individual types of cellulases. This study examines the binding affinity, kinetics of adsorption, and competition of four monocomponent cellulases of Trichoderma reesei during adsorption onto lignin. TrCel7A, TrCel6A, TrCel7B, and TrCel5A were radiolabeled for adsorption experiments on lignin-rich residues (LRRs) isolated from hydrothermally pretreated spruce (L-HPS) and wheat straw (L-HPWS), respectively. On the basis of adsorption isotherms fitted to the Langmuir model, the ranking of binding affinities was TrCel5A > TrCel6A > TrCel7B > TrCel7A on both types of LRRs. The enzymes had a higher affinity to the L-HPS than to the L-HPWS. Adsorption experiments with dilution after 1 and 24 hr and kinetic modeling were performed to quantify any irreversible binding over time. Models with reversible binding parameters fitted well and can explain the results obtained. The adsorption constants obtained from the reversible models agreed with the fitted Langmuir isotherms and suggested that reversible adsorption-desorption existed at equilibrium. Competitive binding experiments showed that individual types of cellulases competed for binding sites on the lignin and the adsorption data fitted the Langmuir adsorption model. Overall, the data strongly indicate that the adsorption of cellulases onto lignin is reversible and the findings have implications for the development of more efficient cellulose degrading enzymes.},
	keywords = {COMPETITION; BIOMASS; ADSORPTION; LIGNIN; Cellulase; Reversible},
	year = {2018},
	eissn = {1097-0290},
	pages = {2869-2880},
	orcid-numbers = {Pihlajaniemi, Ville/0000-0002-8220-6656; Meyer, Anne S./0000-0001-8910-9931}
}

@article{MTMT:27325612,
	title = {Enzymatic Conversion of Sugarcane Lignocellulosic Biomass as a Platform for the Production of Ethanol, Enzymes and Nanocellulose},
	url = {https://m2.mtmt.hu/api/publication/27325612},
	author = {Farinas, Cristiane S and Marconcini, Jose Manoel and Mattoso, Luiz Henrique C},
	doi = {10.7569/JRM.2017.6341578},
	journal-iso = {J RENEW MATERIALS},
	journal = {JOURNAL OF RENEWABLE MATERIALS},
	volume = {6},
	unique-id = {27325612},
	issn = {2164-6325},
	year = {2018},
	eissn = {2164-6341},
	pages = {203-216}
}

@article{MTMT:27579936,
	title = {The effect of amphipilic lignin derivatives addition on enzymatic hydrolysis performance of kraft pulp from sorghum bagasse},
	url = {https://m2.mtmt.hu/api/publication/27579936},
	author = {Fatriasari, Widya and Adi, D T Nugroho and Laksana, R P B and Fajriutami, T and Raniya, R and Ghozali, M and Hermiati, E},
	doi = {10.1088/1755-1315/141/1/012005},
	journal-iso = {IOP CONF SER EARTH AND ENVIRON SCI},
	journal = {IOP CONFERENCE SERIES: EARTH AND ENVIRONMENTAL SCIENCE},
	volume = {141},
	unique-id = {27579936},
	issn = {1755-1307},
	year = {2018},
	eissn = {1755-1315}
}

@article{MTMT:30565455,
	title = {Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification},
	url = {https://m2.mtmt.hu/api/publication/30565455},
	author = {Herbaut, Mickael and Zoghlami, Aya and Paes, Gabriel},
	doi = {10.1186/s13068-018-1267-9},
	journal-iso = {BIOTECHNOL BIOFUELS},
	journal = {BIOTECHNOLOGY FOR BIOFUELS},
	volume = {11},
	unique-id = {30565455},
	issn = {1754-6834},
	abstract = {Background: Improving lignocellulolytic enzymes' diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification. Although many chemical features are considered as detrimental to saccharification, enzymes' dynamics within the cell walls remains poorly explored and understood. To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose. In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification.},
	keywords = {BIOMASS; Porosity; PRETREATMENT; Accessibility; FRAP; SACCHARIFICATION; PEG-rhodamine},
	year = {2018},
	eissn = {1754-6834}
}

@article{MTMT:30565432,
	title = {Feasibility studies with lignin blocking additives in enhancing saccharification and cellulase recovery: Mutant UV-8 of T. verruculosus IIPC 324 a case study},
	url = {https://m2.mtmt.hu/api/publication/30565432},
	author = {Jain, Lavika and Kurmi, Akhilesh Kumar and Agrawal, Deepti},
	doi = {10.1016/j.enzmictec.2018.07.003},
	journal-iso = {ENZYME MICROB TECH},
	journal = {ENZYME AND MICROBIAL TECHNOLOGY},
	volume = {118},
	unique-id = {30565432},
	issn = {0141-0229},
	abstract = {The process economics of fermentable sugar production is dependent on the performance of cellulase cocktail on realistic lignocellulosic biomass and their capability to be recovered and recycled. Feasibility studies were conducted to enhance the digestibility of acid pretreated sugarcane bagasse using novel cellulase cocktail obtained from stable mutant UV-8 of Talaromyces verruculosus IIPC 324 in presence of lignin blocking additives. PEG 6000 was shortlisted as the best additive as it could simultaneously enhance saccharification and overall cellulase recoveries namely cellobiohydrolase, endoglucanase and cellobiase. Addition of 0.3 g PEG 6000/g acidinsoluble lignin content, resulted in 55% and 49.2% saccharification yields in terms of reducing sugars and glucose respectively using this cellulase cocktail (25 mg protein/g cellulose content) after 72 h from acid pre-treated sugarcane bagasse loaded at 7.5%. The study also suggested that the endoglucanase of this mutant was unique with high desorption capability as 85% activity was observed in the saccharified broth devoid of any lignin blocking additive. At its optimum concentration, PEG 6000 was able to retain 94 +/- 0.79% cellobiohydrolase I and 97.97 +/- 1.16% cellobiase enzyme in the saccharified broth which were otherwise lost in residual biomass by similar to 80%, in the absence of this polymeric additive. These results suggest that PEG 6000 was the most promising facilitator for recycling of cellulases obtained from mutant UV-8 of Talaromyces verruculosus IIPC 324 in particular. It paved a way towards the production of cheaper fermentable sugars which serve as a starting raw material for the production of green chemicals and fuels.},
	keywords = {enzymatic saccharification; Solid State fermentation (SSF); Talaromyces verruculosus IIPC 324; Lignin blocking additives (LBA); Surfactants, CCE (concentrated cellulase enzyme); Mutant UV-8},
	year = {2018},
	eissn = {1879-0909},
	pages = {44-49}
}

@article{MTMT:30565453,
	title = {Optimization of Surfactant Addition in Cellulosic Ethanol Process Using Integrated Techno-economic and Life Cycle Assessment for Bioprocess Design},
	url = {https://m2.mtmt.hu/api/publication/30565453},
	author = {Kadhum, Haider Jawad and Rajendran, Karthik and Murthy, Ganti S.},
	doi = {10.1021/acssuschemeng.8b00387},
	journal-iso = {ACS SUSTAIN CHEM ENG},
	journal = {ACS SUSTAINABLE CHEMISTRY & ENGINEERING},
	volume = {6},
	unique-id = {30565453},
	issn = {2168-0485},
	abstract = {Surfactants have been demonstrated to be effective in increasing the cellulase enzyme efficacy and overall enzymatic hydrolysis efficiency. However, the impact of the surfactant addition on the economic viability and environmental impacts of the bioethanol process has not been well-investigated. The objective of this study was to determine the economic and the environmental impacts of using five surfactant types-polyethylene glycol (PEG) 3000, PEG4000, PEG6000, PEG8000, and Tween80-at various concentrations (8%, 5%, 2%, 1%, 0.75%, 0.5%, 0.25%, and 0% (w/w)) during enzymatic hydrolysis and fermentation of pretreated Banagrass. We used an integrated techno-economic and life cycle assesment to guide the selection of optimal surfactant concentration in the bioethanol process. A surfactant concentration of >2% negatively affects the profitability of ethanol, even when there is a statistically significant increase in glucose and ethanol titers. Based on the overall performance indicators for final ethanol, economic viability and environmental impacts, the addition of PEG6000 at 2% (w/w) were determined to be the optimal option. Glucose and ethanol concentrations of 119.2 +/- 5.4 g/L and 55.0 +/- 5.8 g/L, respectively, with an 81.5% cellulose conversion rate, were observed for 2% (w/w) PEG6000. Techno-economic and life cycle analysis indicated that 2% w/w PEG6000 addition resulted in ROI of 3.29% and had reduced the global warming potential by 6 g CO2/MJ ethanol produced.},
	keywords = {HYDROLYSIS; FERMENTATION; Biofuels; Process design; lignocellulose; Techno-economic analysis; sustainable processes; life cycle assessments},
	year = {2018},
	eissn = {2168-0485},
	pages = {13687-13695}
}

@article{MTMT:27579935,
	title = {Effects of Surfactant on the Enzymatic Degradation of Oil Palm Empty Fruit Bunch (OPEFB)},
	url = {https://m2.mtmt.hu/api/publication/27579935},
	author = {Kamsani, Noratiqah and Salleh, Madihah Md and Basri, Siti Aisyah and Mohamad, Shaza Eva and Abd, Aziz Suraini and Kamaruddin, Kamarulzaman},
	doi = {10.1007/s12649-017-9838-8},
	journal-iso = {WASTE BIOMASS VALORI},
	journal = {WASTE AND BIOMASS VALORIZATION},
	volume = {9},
	unique-id = {27579935},
	issn = {1877-2641},
	year = {2018},
	eissn = {1877-265X},
	pages = {845-852}
}

@article{MTMT:30565458,
	title = {Implication of linker length on cell cytotoxicity, pharmacokinetic and toxicity profile of gemcitabine-docetaxel combinatorial dual drug conjugate},
	url = {https://m2.mtmt.hu/api/publication/30565458},
	author = {Kushwah, Varun and Katiyar, Sameer S. and Agrawal, Ashish Kumar and Saraf, Isha and Singh, Inder Pal and Lamprou, Dimitrios A. and Gupta, Ramesh C. and Jain, Sanyog},
	doi = {10.1016/j.ijpharm.2018.07.016},
	journal-iso = {INT J PHARM},
	journal = {INTERNATIONAL JOURNAL OF PHARMACEUTICS},
	volume = {548},
	unique-id = {30565458},
	issn = {0378-5173},
	abstract = {The present study investigates effect of linkers [zero length (without linker), short length linker (glycine and lysine) and long length linker (PEG1000, PEG2000 and PEG3500)] on pharmacokinetics and toxicity of docetaxel (DTX) and gemcitabine (GEM) bio-conjugates. Conjugates were synthesized via carbodiimide chemistry and characterized by H-1 NMR and FTIR. Conjugation of DTX and GEM via linkers showed diverse physio-chemical and plasma stability profile. Cellular uptake mechanism in MCF-7 and MDA-MB-231 cell lines revealed clathrin mediated internalization of bio-conjugates developed by using long length linkers, leading to higher cytotoxicity compared with free drug congeners. DTX-PEG3500-GEM and DTX-PEG2000-GEM demonstrated 4.21 and 3.81-fold higher AUC((0-infinity)) of GEM in comparison with GEM alone. DTX-PEG2000-GEM and DTX-PEG3500-GEM exhibited reduced hepato-, nephro- and haemolytic toxicity as evident via histopathology, biochemical markers and SEM analysis of RBCs. Conclusively, PEG2000 and PEG3500 significantly improved pharmacokinetics without any sign of toxicity and hence can be explored further for the development of dual-drug conjugates for better therapeutic efficacy.},
	keywords = {docetaxel; gemcitabine; Prodrug; bio-conjugation; Dual drug delivery; Linker screening},
	year = {2018},
	eissn = {1873-3476},
	pages = {357-374}
}

@article{MTMT:27579934,
	title = {Effect of Urea on the Enzymatic Hydrolysis of Lignocellulosic Substrate and Its Mechanism},
	url = {https://m2.mtmt.hu/api/publication/27579934},
	author = {Lou, Hongming and Lin, Meilu and Zeng, Meijun and Cai, Cheng and Pang, Yuxia and Yang, Dongjie and Qiu, Xueqing},
	doi = {10.1007/s12155-018-9910-7},
	journal-iso = {BIOENERG RES},
	journal = {BIOENERGY RESEARCH},
	volume = {11},
	unique-id = {27579934},
	issn = {1939-1234},
	year = {2018},
	eissn = {1939-1242},
	pages = {456-465}
}

@article{MTMT:27325613,
	title = {Nonionic surfactants enhanced enzymatic hydrolysis of cellulose by reducing cellulase deactivation caused by shear force and air-liquid interface},
	url = {https://m2.mtmt.hu/api/publication/27325613},
	author = {Lou, Hongming and Zeng, Meijun and Hu, Qiaoyan and Cai, Cheng and Lin, Xuliang and Qiu, Xueqing and Yang, Dongjie and Pang, Yuxia},
	doi = {10.1016/j.biortech.2017.07.066},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {249},
	unique-id = {27325613},
	issn = {0960-8524},
	year = {2018},
	eissn = {1873-2976},
	pages = {1-8}
}

@article{MTMT:30565456,
	title = {The adsorption properties of endoglucanase to lignin and their impact on hydrolysis},
	url = {https://m2.mtmt.hu/api/publication/30565456},
	author = {Lu, Xianqin and Feng, Xiaoting and Li, Xuezhi and Zhao, Jian},
	doi = {10.1016/j.biortech.2018.06.031},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {267},
	unique-id = {30565456},
	issn = {0960-8524},
	abstract = {Nonproductive adsorption of cellulase to lignin dramatically influenced the hydrolysis efficiency of lignocellulose. By comparing the adsorption behaviors of CBH and EG, we found that the adsorption of EG to lignin showed lower adsorption velocity and capacity versus CBH. During the adsorption of EG to lignin, carbohydrate binding domain (CBM) and catalytic domain (CD) both played an important role by a two-step adsorption process, in which CD slowly bond on lignin and developed stronger interaction with lignin. The optimal binding position of EG on lignin was consistent with that on polysaccharide located in the open catalytic tunnel. So, the adsorption of EG to lignin not only limited the movement of enzyme, but also restricted the catalytic ability of enzyme, which dramatically influenced enzymatic hydrolysis. Increasing the proportion of EG in cellulase cocktails or engineering "weak lignin adsorbed" EG was necessary to relieve the influence of lignin adsorption on hydrolysis.},
	keywords = {HYDROLYSIS; ADSORPTION; LIGNIN; Endo-beta-1,4-glucanases},
	year = {2018},
	eissn = {1873-2976},
	pages = {110-116}
}

@article{MTMT:30565452,
	title = {Investigation of different pretreatment methods of Mediterranean-type ecosystem agricultural residues: characterisation of pretreatment products, high-solids enzymatic hydrolysis and bioethanol production},
	url = {https://m2.mtmt.hu/api/publication/30565452},
	author = {Nitsos, Christos and Matsakas, Leonidas and Triantafyllidis, Kostas and Rova, Ulrika and Christakopoulos, Paul},
	doi = {10.1080/17597269.2017.1378988},
	journal-iso = {BIOFUELS-UK},
	journal = {BIOFUELS},
	volume = {9},
	unique-id = {30565452},
	issn = {1759-7269},
	abstract = {Agricultural and agro-industrial lignocellulosic residues represent an important renewable resource for the production of fuels and chemicals towards a bio-based economy. Olive pruning, vineyard pruning and almond shells are important residues from agricultural activities in Mediterranean-type ecosystems. In the current work, bioethanol production from the above three types of agro-residues was studied, focusing on the effect of different pretreatment methods on enzymatic saccharrification efficiency of cellulose and production of second-generation bioethanol. Dilute acid, hydrothermal and steam explosion pretreatments were compared in order to remove hemicellulose and facilitate the subsequent enzymatic hydrolysis of the hemicellulose-deficient biomass to glucose. Enzymatic hydrolysis was performed in a free-fall mixing reactor enabling high solids loading of 23% w/w. This allowed hydrolysis of up to 67% of available cellulose in almond shells and close to 50% in olive pruning samples, and facilitated high ethanol production in the subsequent fermentation step; the highest ethanol concentrations achieved were 47.8 g/L for almond shells after steam explosion and 42 g/L for hydrothermally pretreated olive pruning residue.},
	keywords = {Bioethanol; Steam explosion; Hydrothermal pretreatment; dilute acid; olive pruning; grapevine pruning; almond shells},
	year = {2018},
	eissn = {1759-7277},
	pages = {545-558}
}

@article{MTMT:30565431,
	title = {The Influence of Nonionic Surfactant Adsorption on Enzymatic Hydrolysis of Oil Palm Fruit Bunch},
	url = {https://m2.mtmt.hu/api/publication/30565431},
	author = {Parnthong, Jatuporn and Kungsanant, Suratsawadee and Chavadej, Sumaeth},
	doi = {10.1007/s12010-018-2783-6},
	journal-iso = {APPL BIOCHEM BIOTECH},
	journal = {APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY},
	volume = {186},
	unique-id = {30565431},
	issn = {0273-2289},
	abstract = {Nonionic surfactants have been utilized to improve the enzymatic hydrolysis of lignocellulosic materials. However, the role of surfactant adsorption affecting enzymatic hydrolysis has not been elaborated well. In this work, nonionic surfactants differing in their molecular structures, namely the polyoxyethylene sorbitan monooleate (Tween 80), the secondary alcohol ethoxylate (Tergitol 15-S-9), and the branched alcohol ethoxylate (Tergitol TMN-6), were studied for their effects on the enzymatic hydrolysis of palm fruit bunch (PFB). The PFB was pretreated with a 10% w/v sodium hydroxide solution and then hydrolyzed using the cellulase enzyme from Trichoderma reesei (ATCC 26921) at 50 degrees C and pH5. The optimal conditions providing similar yields of reducing sugar required Tween 80 and Tergitol TMN-6 at 0.25% w/v, while Tergitol 15-S-9 was required at 0.1% w/v. All the surfactants improved the enzymatic conversion efficiency and reduced unproductive binding of the enzyme to lignin. In addition, the adsorption isotherm of cellulase was fit well by the Freundlich isotherm, while adsorption of the three nonionic surfactants agreed well with the Langmuir isotherm. Adsorption capacities of the three nonionic surfactants were consistent with their enhancement efficiencies in hydrolysis. The critical micelle concentration was observed as a key property of nonionic surfactant for adsorption capacity.},
	keywords = {enzymatic hydrolysis; nonionic surfactant; Adsorption isotherm; Hydrophilic-hydrophobic; Palm fruit bunch},
	year = {2018},
	eissn = {1559-0291},
	pages = {895-908}
}

@article{MTMT:30565457,
	title = {Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds},
	url = {https://m2.mtmt.hu/api/publication/30565457},
	author = {Zain, Masniroszaime Md and Mohammad, Abdul Wahab and Harun, Shuhaida and Fauzi, Nurul Aina and Hairom, Nur Hanis Hayati},
	doi = {10.1016/j.indcrop.2018.06.037},
	journal-iso = {IND CROP PROD},
	journal = {INDUSTRIAL CROPS AND PRODUCTS},
	volume = {122},
	unique-id = {30565457},
	issn = {0926-6690},
	abstract = {Due to an increasing demand for more sustainable and renewable resources, there has been strong interest in utilizing biomass as a source for cleaner production of energy and chemicals. In this work, the lignocellulosic elements of oil palm frond (OPF) biomass were assessed as an alternate sugar feedstock for biofuel and bioproducts production. At present, long hydrolysis times and high enzymatic loadings hinder commercialisation and large-scale utilisation of enzymatic hydrolysis of lignocellulosic biomass. Thus, various process parameters of enzymatic hydrolysis of alkaline OPF fibre were investigated in an attempt to improve process performance. In this study, OPF biomass was pretreated with 4.42% NaOH at 100 degrees C for 58.31 min, resulting in significant disruption as characterised by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Alkaline pretreatment of OPF biomass improved enzymatic biodegradability, and glucan recovery by the Cellic Ctec2 enzyme was more effective than the conventional Celluclast 1.5 L cellulase enzyme. Synergistic effects of stirring speed, surfactant Triton X-100 loading, and B-glucosidase supplement on enzymatic hydrolysis were assessed using statistical experimental design. Under optimal conditions (450 rpm, 1.31%, and 0.14 pNPGU/FPU), 88% conversion of glucan was obtained from alkaline OPF, which is equivalent to the conversion from commercial cellulose (microcrystalline cellulose, MCC). Enzymatic hydrolysis of pretreated OPF was further improved at high agitation speeds. Synergy between agitation speed and surfactant loading interactions with beta-glucosidase supplement enhanced glucose production due to the efficient mixing and availability of cellulose to be adsorbed by cellulase.},
	keywords = {GLUCOSE; Cellulase; enzyme hydrolysis; Oil palm frond; Sodium hydroxide pretreatment},
	year = {2018},
	eissn = {1872-633X},
	pages = {617-626},
	orcid-numbers = {Mohammad, Abdul Wahab/0000-0002-9253-8283}
}

@article{MTMT:26756723,
	title = {Improvement of bacterial alpha-amylase production and application using two steps statistical factorial design},
	url = {https://m2.mtmt.hu/api/publication/26756723},
	author = {Ahmed, Samia A and Mostafa, Faten A and Helmy, Wafaa A and Abdel-Naby, Mohamed A},
	doi = {10.1016/j.bcab.2017.03.004},
	journal-iso = {BIOCAT AGRICULT BIOTECH},
	journal = {BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY},
	volume = {10},
	unique-id = {26756723},
	year = {2017},
	eissn = {1878-8181},
	pages = {224-233}
}

@article{MTMT:26756720,
	title = {Effects of the Surface Morphology and Conformations of Lignocellulosic Biomass Biopolymers on Their Nanoscale Interactions with Hydrophobic Self-Assembled Monolayers},
	url = {https://m2.mtmt.hu/api/publication/26756720},
	author = {Arslan, Baran and Egerton, Kirstin and Zhang, Xiao and Abu-Lail, Nehal I},
	doi = {10.1021/acs.langmuir.7b01470},
	journal-iso = {LANGMUIR},
	journal = {LANGMUIR},
	volume = {33},
	unique-id = {26756720},
	issn = {0743-7463},
	year = {2017},
	eissn = {1520-5827},
	pages = {6857-6868}
}

@article{MTMT:26571734,
	title = {Influence of polymer coating on release of l-dopa from core-shell Fe@Au nanoparticle systems},
	url = {https://m2.mtmt.hu/api/publication/26571734},
	author = {Bandyopadhyay, Sulalit and Alvi, Muhammad Awais Ashfaq and Sharma, Anuvansh and Zhu, Kaizheng and Kjoniksen, Anna-Lena and Nystrom, Bo and Glomm, Wilhelm Robert},
	doi = {10.1007/s00396-017-4015-y},
	journal-iso = {COLLOID POLYM SCI},
	journal = {COLLOID AND POLYMER SCIENCE},
	volume = {295},
	unique-id = {26571734},
	issn = {0303-402X},
	year = {2017},
	eissn = {1435-1536},
	pages = {391-402},
	orcid-numbers = {Kjoniksen, Anna-Lena/0000-0003-4864-4043}
}

@article{MTMT:26571732,
	title = {Extracellular expression of alkali tolerant xylanase from Bacillus subtilis Lucky9 in E. coli and application for xylooligosaccharides production from agro-industrial waste},
	url = {https://m2.mtmt.hu/api/publication/26571732},
	author = {Chang, Siyuan and Guo, Yalan and Wu, Bin and He, Bingfang},
	doi = {10.1016/j.ijbiomac.2016.11.032},
	journal-iso = {INT J BIOL MACROMOL},
	journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
	volume = {96},
	unique-id = {26571732},
	issn = {0141-8130},
	year = {2017},
	eissn = {1879-0003},
	pages = {249-256}
}

@article{MTMT:27107452,
	title = {CURRENT CHALLENGES ON THE PRODUCTION AND USE OF CELLULOLYTIC ENZYMES IN THE HYDROLYSIS OF LIGNOCELLULOSIC BIOMASS},
	url = {https://m2.mtmt.hu/api/publication/27107452},
	author = {Florencio, Camila and Badino, Alberto Colli and Farinas, Cristiane Sanchez},
	doi = {10.21577/0100-4042.20170104},
	journal-iso = {QUIM NOVA},
	journal = {QUIMICA NOVA},
	volume = {40},
	unique-id = {27107452},
	issn = {0100-4042},
	year = {2017},
	eissn = {1678-7064},
	pages = {1082-1093}
}

@article{MTMT:27088896,
	title = {Effect of Nonionic Surfactants on Dispersion and Polar Interactions in the Adsorption of Cellulases onto Lignin},
	url = {https://m2.mtmt.hu/api/publication/27088896},
	author = {Jiang, Feng and Qian, Chen and Esker, Alan R and Roman, Maren},
	doi = {10.1021/acs.jpcb.7b07216},
	journal-iso = {J PHYS CHEM B},
	journal = {JOURNAL OF PHYSICAL CHEMISTRY B},
	volume = {121},
	unique-id = {27088896},
	issn = {1520-6106},
	year = {2017},
	eissn = {1520-5207},
	pages = {9607-9620},
	orcid-numbers = {Roman, Maren/0000-0001-6622-8591}
}

@article{MTMT:26571735,
	title = {Enzyme recycling in lignocellulosic biorefineries},
	url = {https://m2.mtmt.hu/api/publication/26571735},
	author = {Jorgensen, Henning and Pinelo, Manuel},
	doi = {10.1002/bbb.1724},
	journal-iso = {BIOFUEL BIOPROD BIOR},
	journal = {BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR},
	volume = {11},
	unique-id = {26571735},
	issn = {1932-104X},
	year = {2017},
	eissn = {1932-1031},
	pages = {150-167},
	orcid-numbers = {Jorgensen, Henning/0000-0003-1220-6893}
}

@article{MTMT:26756722,
	title = {Lignin-derived inhibition of monocomponent cellulases and a xylanase in the hydrolysis of lignocellulosics},
	url = {https://m2.mtmt.hu/api/publication/26756722},
	author = {Kellock, Miriam and Rahikainen, Jenni and Marjamaa, Kaisa and Kruus, Kristiina},
	doi = {10.1016/j.biortech.2017.01.072},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {232},
	unique-id = {26756722},
	issn = {0960-8524},
	year = {2017},
	eissn = {1873-2976},
	pages = {183-191},
	orcid-numbers = {Rahikainen, Jenni/0000-0002-4646-6522}
}

@article{MTMT:26571733,
	title = {Effect of sodium dodecyl sulfate and cetyltrimethylammonium bromide catanionic surfactant on the enzymatic hydrolysis of Avicel and corn stover},
	url = {https://m2.mtmt.hu/api/publication/26571733},
	author = {Lin, Xuliang and Lou, Hongming and Qiu, Xueqing and Pang, Yuxia and Yang, Dongjie},
	doi = {10.1007/s10570-016-1186-5},
	journal-iso = {CELLULOSE},
	journal = {CELLULOSE},
	volume = {24},
	unique-id = {26571733},
	issn = {0969-0239},
	year = {2017},
	eissn = {1572-882X},
	pages = {669-676},
	orcid-numbers = {Lin, Xuliang/0000-0003-2170-5985}
}

@article{MTMT:26756721,
	title = {Lignin-enzyme interaction: Mechanism, mitigation approach, modeling, and research prospects},
	url = {https://m2.mtmt.hu/api/publication/26756721},
	author = {Li, Xiang and Zheng, Yi},
	doi = {10.1016/j.biotechadv.2017.03.010},
	journal-iso = {BIOTECHNOL ADV},
	journal = {BIOTECHNOLOGY ADVANCES},
	volume = {35},
	unique-id = {26756721},
	issn = {0734-9750},
	year = {2017},
	eissn = {1873-1899},
	pages = {466-489}
}

@article{MTMT:27088894,
	title = {Additives enhancing enzymatic hydrolysis of lignocellulosic biomass},
	url = {https://m2.mtmt.hu/api/publication/27088894},
	author = {Rocha-Martin, Javier and Martinez-Bernal, Claudio and Perez-Cobas, Yolanda and Reyes-Sosa, Francisco Manuel and Garcia, Bruno Diez},
	doi = {10.1016/j.biortech.2017.06.132},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {244},
	unique-id = {27088894},
	issn = {0960-8524},
	year = {2017},
	eissn = {1873-2976},
	pages = {48-56}
}

@article{MTMT:27088899,
	title = {Structural changes of lignin in biorefinery pretreatments and consequences to enzyme-lignin interactions},
	url = {https://m2.mtmt.hu/api/publication/27088899},
	author = {Sipponen, Mika Henrikki and Rahikainen, Jenni and Leskinen, Timo and Pihlajaniemi, Ville and Mattinen, Maija-Liisa and Lange, Heiko and Crestini, Claudia and Osterberg, Monika},
	doi = {10.3183/NPPRJ-2017-32-04-p550-571},
	journal-iso = {NORD PULP PAP RES J},
	journal = {NORDIC PULP & PAPER RESEARCH JOURNAL},
	volume = {32},
	unique-id = {27088899},
	issn = {0283-2631},
	year = {2017},
	eissn = {2000-0669},
	pages = {550-571}
}

@article{MTMT:27088895,
	title = {The promotional effect of water-soluble extractives on the enzymatic cellulose hydrolysis of pretreated wheat straw},
	url = {https://m2.mtmt.hu/api/publication/27088895},
	author = {Smit, A T and Huijgen, W J J},
	doi = {10.1016/j.biortech.2017.07.072},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {243},
	unique-id = {27088895},
	issn = {0960-8524},
	year = {2017},
	eissn = {1873-2976},
	pages = {994-999}
}

@article{MTMT:26571731,
	title = {Association of amphipathic lignin derivatives with cellobiohydrolase groups improves enzymatic saccharification of lignocellulosics},
	url = {https://m2.mtmt.hu/api/publication/26571731},
	author = {Yamamoto, Yoko and Cheng, Ningning and Koda, Keiichi and Igarashi, Kiyohiko and Tamai, Yutaka and Uraki, Yasumitsu},
	doi = {10.1007/s10570-017-1214-0},
	journal-iso = {CELLULOSE},
	journal = {CELLULOSE},
	volume = {24},
	unique-id = {26571731},
	issn = {0969-0239},
	year = {2017},
	eissn = {1572-882X},
	pages = {1849-1862}
}

@article{MTMT:27088898,
	title = {Microbial oil production by Mortierella isabellina from corn stover under different pretreatments},
	url = {https://m2.mtmt.hu/api/publication/27088898},
	author = {Zhao, Chen and Deng, Lu and Fang, Hao and Chen, Shaolin},
	doi = {10.1039/c7ra11900c},
	journal-iso = {RSC ADV},
	journal = {RSC ADVANCES},
	volume = {7},
	unique-id = {27088898},
	issn = {2046-2069},
	year = {2017},
	eissn = {2046-2069},
	pages = {56239-56246}
}

@article{MTMT:25668013,
	title = {Impact of delignification on the morphology and the reactivity of steam exploded wheat straw},
	url = {https://m2.mtmt.hu/api/publication/25668013},
	author = {Huron, Maite and Hudebine, Damien and Ferreira, Nicolas Lopes and Lachenal, Dominique},
	doi = {10.1016/j.indcrop.2015.10.040},
	journal-iso = {IND CROP PROD},
	journal = {INDUSTRIAL CROPS AND PRODUCTS},
	volume = {79},
	unique-id = {25668013},
	issn = {0926-6690},
	year = {2016},
	eissn = {1872-633X},
	pages = {104-109}
}

@article{MTMT:26046658,
	title = {THE IMPACT OF PRETREATMENTS ON CELLULOSE FROM SUGAR BEET SHREDS AND ITS SUSCEPTIBILITY TO ENZYMATIC HYDROLYSIS},
	url = {https://m2.mtmt.hu/api/publication/26046658},
	author = {Ivetic, Darjana Z and Antov, Mirjana G},
	journal-iso = {CELL CHEM TECHNOL},
	journal = {CELLULOSE CHEMISTRY AND TECHNOLOGY},
	volume = {50},
	unique-id = {26046658},
	issn = {0576-9787},
	year = {2016},
	eissn = {0576-9787},
	pages = {139-146}
}

@article{MTMT:25668009,
	title = {Development of Thermophilic Tailor-Made Enzyme Mixtures for the Bioconversion of Agricultural and Forest Residues},
	url = {https://m2.mtmt.hu/api/publication/25668009},
	author = {Karnaouri, Anthi and Matsakas, Leonidas and Topakas, Evangelos and Rova, Ulrike and Christakopoulos, Paul},
	doi = {10.3359/fmicb.2016.00177},
	journal-iso = {FRONT MICROBIOL},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {7},
	unique-id = {25668009},
	issn = {1664-302X},
	year = {2016},
	eissn = {1664-302X}
}

@article{MTMT:26233658,
	title = {Toward a fundamental understanding of cellulase-lignin interactions in the whole slurry enzymatic saccharification process},
	url = {https://m2.mtmt.hu/api/publication/26233658},
	author = {Liu, Hao and Sun, Jianliang and Leu, Shao-Yuan and Chen, Shicheng},
	doi = {10.1002/bbb.1670},
	journal-iso = {BIOFUEL BIOPROD BIOR},
	journal = {BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR},
	volume = {10},
	unique-id = {26233658},
	issn = {1932-104X},
	year = {2016},
	eissn = {1932-1031},
	pages = {648-663}
}

@article{MTMT:25668012,
	title = {Effects of lignin and surfactant on adsorption and hydrolysis of cellulases on cellulose},
	url = {https://m2.mtmt.hu/api/publication/25668012},
	author = {Li, Yanfei and Sun, Zongping and Ge, Xiaoyan and Zhang, Junhua},
	doi = {10.1186/s13068-016-0434-0},
	journal-iso = {BIOTECHNOL BIOFUELS},
	journal = {BIOTECHNOLOGY FOR BIOFUELS},
	volume = {9},
	unique-id = {25668012},
	issn = {1754-6834},
	year = {2016},
	eissn = {1754-6834}
}

@article{MTMT:25668010,
	title = {Conformations of Low-Molecular-Weight Lignin Polymers in Water},
	url = {https://m2.mtmt.hu/api/publication/25668010},
	author = {Petridis, Loukas and Smith, Jeremy C},
	doi = {10.1002/cssc.201501350},
	journal-iso = {CHEMSUSCHEM},
	journal = {CHEMSUSCHEM},
	volume = {9},
	unique-id = {25668010},
	issn = {1864-5631},
	year = {2016},
	eissn = {1864-564X},
	pages = {289-295},
	orcid-numbers = {Petridis, Loukas/0000-0001-8569-060X}
}

@article{MTMT:26399778,
	title = {Cellulase adsorption on lignin: A roadblock for economic hydrolysis of biomass},
	url = {https://m2.mtmt.hu/api/publication/26399778},
	author = {Saini, Jitendra Kumar and Patel, Anil Kumar and Adsul, Mukund and Singhania, Reeta Rani},
	doi = {10.1016/j.renene.2016.03.089},
	journal-iso = {RENEW ENERGY},
	journal = {RENEWABLE ENERGY},
	volume = {98},
	unique-id = {26399778},
	issn = {0960-1481},
	year = {2016},
	eissn = {1879-0682},
	pages = {29-42}
}

@article{MTMT:25668011,
	title = {An integrated system for fractionation and hydrolysis of sugarcane bagasse using heterogeneous catalysts in aqueous biphasic system},
	url = {https://m2.mtmt.hu/api/publication/25668011},
	author = {Sakdaronnarong, Chularat and Saengsawang, Arisarak and Siriyutta, Asanee and Jonglertjunya, Woranart and Nasongkla, Norased and Laosiripojana, Navadol},
	doi = {10.1016/j.cej.2015.09.098},
	journal-iso = {CHEM ENG J},
	journal = {CHEMICAL ENGINEERING JOURNAL},
	volume = {285},
	unique-id = {25668011},
	issn = {1385-8947},
	year = {2016},
	eissn = {1873-3212},
	pages = {144-156},
	orcid-numbers = {Sakdaronnarong, Chularat/0000-0002-0195-2236}
}

@article{MTMT:26046657,
	title = {Engineering Cel7A carbohydrate binding module and linker for reduced lignin inhibition},
	url = {https://m2.mtmt.hu/api/publication/26046657},
	author = {Strobel, Kathryn L and Pfeiffer, Katherine A and Blanch, Harvey W and Clark, Douglas S},
	doi = {10.1002/bit.25889},
	journal-iso = {BIOTECHNOL BIOENG},
	journal = {BIOTECHNOLOGY AND BIOENGINEERING},
	volume = {113},
	unique-id = {26046657},
	issn = {0006-3592},
	year = {2016},
	eissn = {1097-0290},
	pages = {1369-1374}
}

@article{MTMT:26233659,
	title = {A novel film-pore-surface diffusion model to explain the enhanced enzyme adsorption of corn stover pretreated by ultrafine grinding},
	url = {https://m2.mtmt.hu/api/publication/26233659},
	author = {Zhang, Haiyan and Chen, Longjian and Lu, Minsheng and Li, Junbao and Han, Lujia},
	doi = {10.1186/s13068-016-0602-2},
	journal-iso = {BIOTECHNOL BIOFUELS},
	journal = {BIOTECHNOLOGY FOR BIOFUELS},
	volume = {9},
	unique-id = {26233659},
	issn = {1754-6834},
	year = {2016},
	eissn = {1754-6834}
}

@article{MTMT:26233660,
	title = {Investigation of adsorption kinetics and isotherm of cellulase and beta-glucosidase on lignocellulosic substrates},
	url = {https://m2.mtmt.hu/api/publication/26233660},
	author = {Zheng, Yi and Zhang, Ruihong and Pan, Zhongli},
	doi = {10.1016/j.biombioe.2016.04.014},
	journal-iso = {BIOMASS BIOENERGY},
	journal = {BIOMASS & BIOENERGY},
	volume = {91},
	unique-id = {26233660},
	issn = {0961-9534},
	year = {2016},
	eissn = {1873-2909},
	pages = {1-9}
}

@article{MTMT:25668008,
	title = {Polymer-enhanced enzymatic microalgal cell disruption for lipid and sugar recovery},
	url = {https://m2.mtmt.hu/api/publication/25668008},
	author = {Zheng, Yi and Xiao, Rui and Roberts, Mark},
	doi = {10.1016/j.algal.2016.01.010},
	journal-iso = {ALGAL RES},
	journal = {ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS},
	volume = {14},
	unique-id = {25668008},
	issn = {2211-9264},
	year = {2016},
	eissn = {2211-9264},
	pages = {100-108}
}

@article{MTMT:25668022,
	title = {FeCl3 Pretreatment of Three Lignocellulosic Biomass for Ethanol Production},
	url = {https://m2.mtmt.hu/api/publication/25668022},
	author = {Chen, Liheng and Chen, Rong and Fu, Shiyu},
	doi = {10.1021/acssuschemeng.5b00377},
	journal-iso = {ACS SUSTAIN CHEM ENG},
	journal = {ACS SUSTAINABLE CHEMISTRY & ENGINEERING},
	volume = {3},
	unique-id = {25668022},
	issn = {2168-0485},
	year = {2015},
	eissn = {2168-0485},
	pages = {1794-1800}
}

@article{MTMT:25668015,
	title = {Interactions between Cellulolytic Enzymes with Native, Autohydrolysis, and Technical Lignins and the Effect of a Polysorbate Amphiphile in Reducing Nonproductive Binding},
	url = {https://m2.mtmt.hu/api/publication/25668015},
	author = {Fritz, Consuelo and Ferrer, Ana and Salas, Carlos and Jameel, Hasan and Rojas, Orlando J},
	doi = {10.1021/acs.biomac.5b01203},
	journal-iso = {BIOMACROMOLECULES},
	journal = {BIOMACROMOLECULES},
	volume = {16},
	unique-id = {25668015},
	issn = {1525-7797},
	year = {2015},
	eissn = {1526-4602},
	pages = {3878-3888}
}

@article{MTMT:25668025,
	title = {Fuels and Chemicals from Lignocellulosic Biomass: An Integrated Biorefinery Approach},
	url = {https://m2.mtmt.hu/api/publication/25668025},
	author = {Ghosh, Debashish and Dasgupta, Diptarka and Agrawal, Deepti and Kaul, Savita and Adhikari, Dilip Kumar and Kurmi, Akhilesh Kumar and Arya, Pankaj K and Bangwal, Dinesh and Negi, Mahendra Singh},
	doi = {10.1021/acs.energyfuels.5b00144},
	journal-iso = {ENERG FUEL},
	journal = {ENERGY AND FUELS},
	volume = {29},
	unique-id = {25668025},
	issn = {0887-0624},
	year = {2015},
	eissn = {1520-5029},
	pages = {3149-3157}
}

@article{MTMT:25668027,
	title = {Cellobiohydrolase and endoglucanase respond differently to surfactants during the hydrolysis of cellulose},
	url = {https://m2.mtmt.hu/api/publication/25668027},
	author = {Hsieh, Chia-wen C and Cannella, David and Jorgensen, Henning and Felby, Claus and Thygesen, Lisbeth G},
	doi = {10.1186/s13068-015-0242-y},
	journal-iso = {BIOTECHNOL BIOFUELS},
	journal = {BIOTECHNOLOGY FOR BIOFUELS},
	volume = {8},
	unique-id = {25668027},
	issn = {1754-6834},
	year = {2015},
	eissn = {1754-6834},
	orcid-numbers = {Jorgensen, Henning/0000-0003-1220-6893}
}

@article{MTMT:25668020,
	title = {Implications of differences in macromolecular composition of stem fractions for processing of Scots pine},
	url = {https://m2.mtmt.hu/api/publication/25668020},
	author = {Johansson, Sara and Carlqvist, Karin and Kataria, Rashmi and Ulvcrona, Thomas and Bergsten, Urban and Arshadi, Mehrdad and Galbe, Mats and Liden, Gunnar},
	doi = {10.1007/s00226-015-0739-3},
	journal-iso = {WOOD SCI TECHNOL},
	journal = {WOOD SCIENCE AND TECHNOLOGY},
	volume = {49},
	unique-id = {25668020},
	issn = {0043-7719},
	year = {2015},
	eissn = {1432-5225},
	pages = {1037-1054}
}

@article{MTMT:24662590,
	title = {Adsorption of Enzyme Onto Lignins of Liquid Hot Water Pretreated Hardwoods},
	url = {https://m2.mtmt.hu/api/publication/24662590},
	author = {Ko, JK and Ximenes, E and Kim, Y and Ladisch, MR},
	doi = {10.1002/bit.25359},
	journal-iso = {BIOTECHNOL BIOENG},
	journal = {BIOTECHNOLOGY AND BIOENGINEERING},
	volume = {112},
	unique-id = {24662590},
	issn = {0006-3592},
	year = {2015},
	eissn = {1097-0290},
	pages = {447-456}
}

@article{MTMT:25668021,
	title = {Benefits from additives and xylanase during enzymatic hydrolysis of bamboo shoot and mature bamboo},
	url = {https://m2.mtmt.hu/api/publication/25668021},
	author = {Li, Kena and Wang, Xiao and Wang, Jingfeng and Zhang, Junhua},
	doi = {10.1016/j.biortech.2015.05.100},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {192},
	unique-id = {25668021},
	issn = {0960-8524},
	year = {2015},
	eissn = {1873-2976},
	pages = {424-431}
}

@article{MTMT:25668024,
	title = {Lignin-based polyoxyethylene ether enhanced enzymatic hydrolysis of lignocelluloses by dispersing cellulase aggregates},
	url = {https://m2.mtmt.hu/api/publication/25668024},
	author = {Lin, Xuliang and Qiu, Xueqing and Yuan, Long and Li, Zihao and Lou, Hongming and Zhou, Mingsong and Yang, Dongjie},
	doi = {10.1016/j.biortech.2015.02.067},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {185},
	unique-id = {25668024},
	issn = {0960-8524},
	year = {2015},
	eissn = {1873-2976},
	pages = {165-170}
}

@article{MTMT:25668023,
	title = {Effect of additives on adsorption and desorption behavior of xylanase on acid-insoluble lignin from corn stover and wheat straw},
	url = {https://m2.mtmt.hu/api/publication/25668023},
	author = {Li, Yanfei and Ge, Xiaoyan and Sun, Zongping and Zhang, Junhua},
	doi = {10.1016/j.biortech.2015.03.058},
	journal-iso = {BIORESOUR TECHNOL},
	journal = {BIORESOURCE TECHNOLOGY},
	volume = {186},
	unique-id = {25668023},
	issn = {0960-8524},
	year = {2015},
	eissn = {1873-2976},
	pages = {316-320}
}

@article{MTMT:25668030,
	title = {The potential of CAM crops as a globally significant bioenergy resource: moving from 'fuel or food' to 'fuel and more food'},
	url = {https://m2.mtmt.hu/api/publication/25668030},
	author = {Mason, P Michael and Glover, Katherine and Smith, J Andrew C and Willis, Kathy J and Woods, Jeremy and Thompson, Ian P},
	doi = {10.1039/c5ee00242g},
	journal-iso = {ENERG ENVIRON SCI},
	journal = {ENERGY & ENVIRONMENTAL SCIENCE},
	volume = {8},
	unique-id = {25668030},
	issn = {1754-5692},
	year = {2015},
	eissn = {1754-5706},
	pages = {2320-2329}
}