TY - JOUR AU - Romani, Aloia AU - Del-Rio, Pablo G. AU - Rubira, Alexandre AU - Perez, Maria Jose AU - Garrote, Gil TI - Co-valorization of discarded wood pinchips and sludge from the pulp and paper industry for production of advanced biofuels JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 209 PY - 2024 PG - 9 SN - 0926-6690 DO - 10.1016/j.indcrop.2023.117992 UR - https://m2.mtmt.hu/api/publication/34940874 ID - 34940874 LA - English DB - MTMT ER - TY - JOUR AU - Esonye, Chizoo AU - Esonye, Constance Okechukwu AU - Agha, Emmanuel Obiahu AU - Ume, Cyril Sunday AU - Njemanze, Chizoma Vivian AU - Eyisi, Chimezie Emmanuel AU - Adepoju, Tunde Folunsho TI - The 2022 Russia invasion on Ukraine: The biofuel energy security challenge on Ukraine and some related countries JF - HELIYON J2 - HELIYON VL - 9 PY - 2023 IS - 11 PG - 17 SN - 2405-8440 DO - 10.1016/j.heliyon.2023.e21483 UR - https://m2.mtmt.hu/api/publication/34609146 ID - 34609146 AB - Europe, United States and other countries make moves to compensate for the loss of Ukraine's exports by diverting crops meant for biofuels into food production and to relax some of the biofuels mandates due to the challenges of 2022 Russian invasion on Ukraine (RIU). Ukraine as a leading exporter of cereals and oil crops in the world has suffered reduction in exports of grain products since the inception of the invasion. This work critically evaluates the immediate and remote potential effects of the RIU on Ukraine's biofuel capacity and potentials, energy strategies and policies, direct impacts of the war on Ukraine and Russia and other countries' biofuels policies were evaluated. Ukraine is found to be a very important big player on global biofuels energy security and policies. It was observed that there are more significant impacts of the war on Ukraine biofuel industry than that of Russia due to very low priority on the development of Liquid biofuels (LBs) in Russia. Recommendations based on the concepts of national energy security and independence were elucidated. Post invasion-strategies such as development of framework for management of post-war waste, ensuring strict adherence to EU biofuels sustainable directives, and implementation of Bioplus-project for Ukraine government were underpinned. This article provides useful information, guidelines and directives that would enable Ukraine government and other countries facing energy insecurity and political crises to restore energy independence and national economy. LA - English DB - MTMT ER - TY - JOUR AU - Gomez, James A. AU - Matallana, Luis G. AU - Teixeira, Jose A. AU - Sanchez, Oscar J. TI - A framework for the design of sustainable multi-input second-generation biorefineries through process simulation: A case study for the valorization of lignocellulosic and starchy waste from the plantain agro-industry JF - CHEMICAL ENGINEERING RESEARCH AND DESIGN J2 - CHEM ENG RES DES VL - 195 PY - 2023 SP - 551 EP - 571 PG - 21 SN - 0263-8762 DO - 10.1016/j.cherd.2023.06.004 UR - https://m2.mtmt.hu/api/publication/34278442 ID - 34278442 AB - The plantain agro-industry generates different residues in the harvest and post-harvest stages. Therefore, new processes for its valorization are required. The aim of this research was to propose a general methodological framework for the design and analysis of multi -input biorefineries for the valorization of residues with a high content of lignocellulosic and starchy materials. This approach was based on the independent processing of the starchy and lignocellulosic materials in the first steps of the biorefinery and relies on process simulation and hierarchic process design procedures. A case study for the design of a multi-input biorefinery for the valorization of residues from the plantain agro-in-dustry was performed by applying the framework proposed. The biorefinery was simu-lated using SuperPro Designer software. The results obtained suggest that the best alternative for the valorization of plantain residues under Colombian conditions corre-sponded to the following distribution of key residues: 100% leaf sheaths processed into the natural fiber production section, and 25% peels and 25% rachis processed into the starch extraction and liquefaction section; with this distribution, an NPV of $21,348,000 can be achieved. This work shows that waste from the plantain agro-industry exhibits high po-tential as a feedstock for the production of value-added products.& COPY; 2023 The Authors. Published by Elsevier Ltd on behalf of Institution of Chemical Engineers. This is an open access article under the CC BY-NC-ND license (http://creati-vecommons.org/licenses/by-nc-nd/4.0/). LA - English DB - MTMT ER - TY - JOUR AU - Kumar, Naveen AU - Yadav, Anita AU - Singh, Gulab AU - Singh, Ajay AU - Kumar, Pankaj AU - Aggarwal, Neeraj K. TI - Comparative study of ethanol production from sodium hydroxide pretreated rice straw residue using Saccharomyces cerevisiae and Zymomonas mobilis JF - ARCHIVES OF MICROBIOLOGY J2 - ARCH MICROBIOL VL - 205 PY - 2023 IS - 4 PG - 11 SN - 0302-8933 DO - 10.1007/s00203-023-03468-1 UR - https://m2.mtmt.hu/api/publication/33891816 ID - 33891816 AB - Rice straw is a suitable alternative to a cheaper carbohydrate source for the production of ethanol. For pretreatment efficiency, different sodium hydroxide concentrations (0.5-2.5% w/v) were tested. When compared to other concentrations, rice straw processed with 2% NaOH (w/v) yielded more sugar (8.17 +/- 0.01 mg/ml). An alkali treatment induces effective delignification and swelling of biomass. The pretreatment of rice straw with 2% sodium hydroxide (w/v) is able to achieve 55.34% delignification with 53.30% cellulose enrichment. The current study shows the effectiveness of crude cellulolytic preparation from Aspergillus niger resulting in 80.51 +/- 0.4% cellulose hydrolysis. Rice straw hydrolysate was fermented using ethanologenic Saccharomyces cerevisiae (yeast) and Zymomonas mobilis (bacteria). Overall, superior efficiency of sugar conversion to ethanol 70.34 +/- 0.3% was obtained with the yeast compared to bacterial strain 39.18 +/- 0.5%. The current study showed that pretreatment with sodium hydroxide is an effective method for producing ethanol from rice straw and yeast strain S. cerevisiae having greater fermentative potential for bioethanol production than bacterial strain Z. mobilis. LA - English DB - MTMT ER - TY - JOUR AU - Li, Hong-Fei AU - Tian, Linyue AU - Lian, Guoli AU - Fan, Li-Hai AU - Li, Zheng-Jun TI - Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch JF - FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY J2 - FRONT BIOENG BIOTECHNOL VL - 11 PY - 2023 PG - 10 SN - 2296-4185 DO - 10.3389/fbioe.2023.1130368 UR - https://m2.mtmt.hu/api/publication/33891818 ID - 33891818 AB - Vibrio alginolyticus LHF01 was engineered to efficiently produce poly-3-hydroxybutyrate (PHB) from starch in this study. Firstly, the ability of Vibrio alginolyticus LHF01 to directly accumulate PHB using soluble starch as the carbon source was explored, and the highest PHB titer of 2.06 g/L was obtained in 18 h shake flask cultivation. Then, with the analysis of genomic information of V. alginolyticus LHF01, the PHB synthesis operon and amylase genes were identified. Subsequently, the effects of overexpressing PHB synthesis operon and amylase on PHB production were studied. Especially, with the co-expression of PHB synthesis operon and amylase, the starch consumption rate was improved and the PHB titer was more than doubled. The addition of 20 g/L insoluble corn starch could be exhausted in 6-7 h cultivation, and the PHB titer was 4.32 g/L. To the best of our knowledge, V. alginolyticus was firstly engineered to produce PHB with the direct utilization of starch, and this stain can be considered as a novel host to produce PHB using starch as the raw material. LA - English DB - MTMT ER - TY - JOUR AU - Mikulski, Dawid AU - Klosowski, Grzegorz TI - High-pressure microwave-assisted pretreatment of softwood, hardwood and non-wood biomass using different solvents in the production of cellulosic ethanol JF - BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS J2 - BIOTECHN BIOFUEL BIOPROD VL - 16 PY - 2023 IS - 1 PG - 19 SN - 2731-3654 DO - 10.1186/s13068-023-02272-9 UR - https://m2.mtmt.hu/api/publication/33891817 ID - 33891817 AB - BackgroundPretreatment is an indispensable stage of the preparation of lignocellulosic biomass with key significance for the effectiveness of hydrolysis and the efficiency of the production of cellulosic ethanol. A significant increase in the susceptibility of the raw material to further degradation can be attained as a result of effective delignification in high-pressure conditions. With this in mind, a method of high-pressure pretreatment using microwave radiation and various solvents (water, 40% w/v NaCS, 1% v/v H2SO4, 1% w/v NaOH or 60% v/v EtOH with an addition of 1% v/v H2SO4) was developed, enabling the acquisition of biomass with an increased susceptibility to the process of enzymatic hydrolysis. The medium obtained in this way can be used for the production of cellulosic ethanol via high-gravity technology (lignocellulosic media containing from 15 to 20% dry weight of biomass). For every type of biomass (pine chips, beech chips and wheat straw), a solvent was selected to be used during the pretreatment, guaranteeing the acquisition of a medium highly susceptible to the process of enzymatic hydrolysis.ResultsThe highest efficiency of the hydrolysis of biomass, amounting to 71.14 +/- 0.97% (glucose concentration 109.26 +/- 3.49 g/L) was achieved for wheat straw subjected to microwave-assisted pretreatment using 40% w/v NaCS. Fermentation of this medium produced ethanol concentration at the level of 53.84 +/- 1.25 g/L. A slightly lower effectiveness of enzymatic hydrolysis (62.21 +/- 0.62%) was achieved after high-pressure microwave-assisted pretreatment of beech chips using 1% w/v NaOH. The hydrolysate contained glucose in the concentration of 91.78 +/- 1.91 g/L, and the acquired concentration of ethanol after fermentation amounted to 49.07 +/- 2.06 g/L. In the case of pine chips, the most effective delignification was achieved using 60% v/v EtOH with the addition of 1% v/v H2SO4, but after enzymatic hydrolysis, the concentration of glucose in hydrolysate was lower than in the other raw materials and amounted to 39.15 +/- 1.62 g/L (the concentration of ethanol after fermentation was ca. 19.67 +/- 0.98 g/L). The presence of xylose and galactose was also determined in the obtained fermentation media. The highest initial concentration of these carbohydrates (21.39 +/- 1.44 g/L) was observed in beech chips media after microwave-assisted pretreatment using NaOH. The use of wheat straw after pretreatment using EtOH with an addition of 1% v/v H2SO4 for the preparation of fermentation medium, results in the generation of the initial concentration of galactose and xylose at the level of 19.03 +/- 0.38 g/L.ConclusionThe achieved results indicate a high effectiveness of the enzymatic hydrolysis of the biomass subjected to high-pressure microwave-assisted pretreatment. The final effect depends on the combined use of correctly selected solvents for the different sources of lignocellulosic biomass. On the basis of the achieved results, we can say that the presented method indicates a very high potential in the area of its use for the production of cellulosic ethanol involving high-gravity technology. LA - English DB - MTMT ER - TY - JOUR AU - Neudecker, Felix AU - Jakob, Matthias AU - Bodner, Sabine C. AU - Keckes, Jozef AU - Buerstmayr, Hermann AU - Gindl-Altmutter, Wolfgang TI - Delignification and Densification as a Route to Enable the Use of Wheat Straw for Structural Materials JF - ACS SUSTAINABLE CHEMISTRY & ENGINEERING J2 - ACS SUSTAIN CHEM ENG PY - 2023 PG - 9 SN - 2168-0485 DO - 10.1021/acssuschemeng.3c01375 UR - https://m2.mtmt.hu/api/publication/33891815 ID - 33891815 AB - Cereal straw is an abundant byproduct of crop production. Despite little current use in materials, the biologically optimized structure, which carries the weight of a structure up to 1.5 m high and protects it against environmental impacts, shows considerable potential. In order to be able to use this renewable material for structural purposes, barriers such as small dimensions and a low density have to be overcome. In the present study, lignin was partially removed from wheat straw using an alkaline solution with the aim of increasing the relative cellulose content and improving malleability. Thereafter, a homogeneous, dense, and multi layered material was produced by densification at elevated temperatures. Outstanding improvements in mechanical performance were obtained compared to the untreated raw material. For the optimum variant, tensile strength increased by a factor of three (average strength 218 MPa) and elastic modulus increased by a factor of four (average 13 GPa), which is within the range of established wood-based structural materials. We propose that mild alkaline treatment as carried out in the present study may significantly facilitate the development of high-value structural materials based on little utilized cereal straw. LA - English DB - MTMT ER - TY - JOUR AU - Periyasamy, Selvakumar AU - Isabel, J. Beula AU - Kavitha, S. AU - Karthik, V. AU - Mohamed, Badr A. AU - Gizaw, Desta Getachew AU - Sivashanmugam, P. AU - Aminabhavi, Tejraj M. TI - Recent advances in consolidated bioprocessing for conversion of lignocellulosic biomass into bioethanol-A review JF - CHEMICAL ENGINEERING JOURNAL J2 - CHEM ENG J VL - 453 PY - 2023 PG - 16 SN - 1385-8947 DO - 10.1016/j.cej.2022.139783 UR - https://m2.mtmt.hu/api/publication/33891819 ID - 33891819 AB - As the world's population grows, it will be necessary to make long-term technical advancements to meet energy needs without jeopardizing the planet's scarce resources. There are several benefits to using renewable energy systems, such as stabilizing energy supply and demand, ensuring food security and economic stability, and protecting the environment from pollution. Bioethanol is a possible alternative for a renewable and long-term energy source. However, for long-term survival and economic viability, it is critical to focus on lower-cost ethanol production for this fuel to compete with petroleum. To make biofuel commercially viable, converting lignocellulose to bioethanol might be more cost-effective, environmentally friendly, and efficient, but it would still require substantial process research and optimization. The production cost of value-added products, i.e., bioethanol, can be reduced by using consolidated bioprocessing to combine enzyme synthesis and secretion with the hydrolysis of polysaccharides and fermentation of accessible sugars in a single operation. This review focuses on biomass pretreatments, process enhancements, recombinant microbial catalysts and enzymes, and metabolic engineering to consolidate bioprocessing. Besides, it delivers an overview to facilitate future research in ligno-cellulosic biomass for bioethanol production. LA - English DB - MTMT ER - TY - JOUR AU - Srivastava, Rajesh K. AU - Sarangi, Prakash Kumar AU - Shadangi, Krushna Prasad AU - Sasmal, Soumya AU - Gupta, Vijai Kumar AU - Govarthanan, Muthusamy AU - Sahoo, Uttam Kumar AU - Subudhi, Sanjukta TI - Biorefineries development from agricultural byproducts: Value addition and circular bioeconomy JF - SUSTAINABLE CHEMISTRY AND PHARMACY J2 - SUSTAIN CHEM PHARM VL - 32 PY - 2023 PG - 22 SN - 2352-5541 DO - 10.1016/j.scp.2023.100970 UR - https://m2.mtmt.hu/api/publication/34278443 ID - 34278443 AB - The increase in the human population in the world, industrialization, and rapid urbanization cre-ates a big challenge for the agricultural revolution. Hence, to enhance the crop's productivity modern agricultural technology, hybrid seeds; management of full irrigation facilities, and the use of various fertilizers, and pesticides are being adopted. Though such advanced techniques en-hance productivity, they also produce a huge amount of agro-waste. The proper waste manage-ment of such wastes is a headache for the farmers due to the lack of dumping lands, proper trans-portation, removal facilities, and lack of planned utilization. Since direct burning of the wastes do air, water, and soil pollution, a systematic utilization of these cheap wastes for the development of value-added products and chemicals will promote a clean and healthy environment for the bi-otic and abiotic components. A proper, efficient, and effective treatment system can break down waste biomass into simple forms and can be easily consumed by our natural and engineered mi-crobial systems to produce bio-chemicals and bio-fuels. Value-added products from agro wastes are now needed every hour as far as the sustainable production of energy and biochemical is an important concern. Agro-waste utilization-based biorefineries can provide a big pillar for the de-velopment of a circular bio-economy. The review emphasizes the value-added products gener-ated from agricultural byproducts/waste residue's utility, towards sustainability and a clean envi-ronment. LA - English DB - MTMT ER - TY - JOUR AU - Bansal, Riya AU - Katyal, Priya AU - Jain, Deepali TI - Enzymatic and Acidic Hydrolysis of Cull Potatoes for Production of Fermentable Sugars JF - STARCH-STARKE J2 - STARCH-STARKE VL - 74 PY - 2022 IS - 1-2 PG - 4 SN - 0038-9056 DO - 10.1002/star.202100202 UR - https://m2.mtmt.hu/api/publication/33003549 ID - 33003549 AB - Cull potato is an under-utilized biomass being produced in different states of India. Using cull potatoes as a substrate for bioethanol production can overcome the post-harvest losses as well as making the process economical. In the present study, comparative hydrolysis of cull potatoes is carried out using enzymes and hydrochloric acid to study the effect of variables on saccharification efficiency. For enzymatic hydrolysis, samples are liquefied using crude enzyme from Bacillus sp. and then saccharification of liquefied samples is optimized using central composite rotatable design (CCRD) in Response surface methodology (RSM) w.r.t pH (4-7), temperature (25-40 degrees C) and incubation period (15-60 min) at 5% substrate concentration with glucoamylase of Aspergillus niger. Acidic hydrolysis is performed at different acid concentration, i.e., 1-5 (v/v) at variable time intervals (15-60 min) with 1-5% substrate concentration at 90 degrees C. Highest saccharification efficiency is observed with acidic hydrolysis 85.2% at substrate concentration of (4%) and at acid concentration (4% v/v) after 45 min of incubation, while with enzymatic hydrolysis it is very close to acidic hydrolysis, i.e., 83.2% at 25 degrees C temperature and at pH 4 after 30 min of incubation. LA - English DB - MTMT ER - TY - JOUR AU - Geng, Binan AU - Liu, Shuyi AU - Chen, Yunhao AU - Wu, Yalun AU - Wang, Yi AU - Zhou, Xuan AU - Li, Han AU - Li, Mian AU - Yang, Shihui TI - A plasmid-free Zymomonas mobilis mutant strain reducing reactive oxygen species for efficient bioethanol production using industrial effluent of xylose mother liquor JF - FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY J2 - FRONT BIOENG BIOTECHNOL VL - 10 PY - 2022 PG - 14 SN - 2296-4185 DO - 10.3389/fbioe.2022.1110513 UR - https://m2.mtmt.hu/api/publication/33873645 ID - 33873645 AB - Genome minimization is an effective way for industrial chassis development. In this study, Zymomonas mobilis ZMNP, a plasmid-free mutant strain of Z. mobilis ZM4 with four native plasmids deleted, was constructed using native type I-F CRISPR-Cas system. Cell growth of ZMNP under different temperatures and industrial effluent of xylose mother liquor were examined to investigate the impact of native plasmid removal. Despite ZMNP grew similarly as ZM4 under different temperatures, ZMNP had better xylose mother liquor utilization than ZM4. In addition, genomic, transcriptomic, and proteomic analyses were applied to unravel the molecular changes between ZM4 and ZMNP. Whole-genome resequencing result indicated that an S267P mutation in the C-terminal of OxyR, a peroxide-sensing transcriptional regulator, probably alters the transcription initiation of antioxidant genes for stress responses. Transcriptomic and proteomic studies illustrated that the reason that ZMNP utilized the toxic xylose mother liquor better than ZM4 was probably due to the upregulation of genes in ZMNP involving in stress responses as well as cysteine biosynthesis to accelerate the intracellular ROS detoxification and nucleic acid damage repair. This was further confirmed by lower ROS levels in ZMNP compared to ZM4 in different media supplemented with furfural or ethanol. The upregulation of stress response genes due to the OxyR mutation to accelerate ROS detoxification and DNA/RNA repair not only illustrates the underlying mechanism of the robustness of ZMNP in the toxic xylose mother liquor, but also provides an idea for the rational design of synthetic inhibitor-tolerant microorganisms for economic lignocellulosic biochemical production. LA - English DB - MTMT ER - TY - JOUR AU - Novo, Przulj AU - Vesna, Tunguz AU - Zoran, Jovovic AU - Ana, Velimirovic TI - THE SIGNIF ICANCE OF HARVEST RESIDUES IN THE SUSTAINABLE MANAGEMENT OF ARABLE LAND. II. HARVEST RESIDUES MANAGEMENT JF - ARHIV ZA TEHNICKE NAUKE / ARCHIVES FOR TECHNICAL SCIENCES J2 - ARCH TECHN SCI PY - 2022 IS - 27 SP - 49 EP - 56 PG - 8 SN - 1840-4855 DO - 10.7251/afts.2022.1427.049PCOBISS.RS-ID137253377 UR - https://m2.mtmt.hu/api/publication/34278444 ID - 34278444 AB - Harvest residues (HR) are one of the important aspects of sustainable management in agriculture, representing a significant portion of organic matter (OM) that can be retained or removed from agro-ecosystems. There are several ways to manage plant residues: (i) burning, (ii) incorporation in soil, (iii) leaving plant residues after harvest on the soil surface in form of mulch, (iv) undersowing crops in HR and (v) baling and removing HR from the plot. Burning is the most unreasonable action in agricultural production, which is prohibited by legal regulations in force. The burning means a complete loss of OM -N and S are irretrievably lost in the air, mineral substances remain in ash, and microorganisms in the soil surface layer are destroyed. Incorporation of HR may be complete or partial, depending on the soil cultivation method applied.This HR management method has a number of soil benefits: increases content of nutrients, OM and humus; increases microbiological activity; improves soil water, air and physical properties; improves soil structure, ultimately contributing to the increase in soil fertility and plant growth. Two practical approaches that were once common, have been neglected. Namely, undersowing and mixed cultivation where various species are simultaneously grown with or between plantings of a main crop. Removal of HR indirectly leads to a decrease of the organic matter in the soil. In essence, there is a need to determine the size of acceptors and the return rates of organic residues of different quality, and to increase the efficiency of nutrient cycling from residues through various land acceptors and crops, with minimal losses from the system. LA - English DB - MTMT ER - TY - JOUR AU - Qiao, Jie AU - Cui, Haiyang AU - Wang, Minghui AU - Fu, Xianshen AU - Wang, Xinyue AU - Li, Xiujuan AU - Huang, He TI - Integrated biorefinery approaches for the industrialization of cellulosic ethanol fuel JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 360 PY - 2022 PG - 13 SN - 0960-8524 DO - 10.1016/j.biortech.2022.127516 UR - https://m2.mtmt.hu/api/publication/33224765 ID - 33224765 AB - Lignocellulosic biomass is an abundant and sustainable raw material, but its conversion into ethanol fuel has not yet achieved large-scale industrialization and economic benefits. Integrated biorefineries have been widely identified as the key to achieving this goal. Here, four promising routes were summarized to assemble the new industrial plants for cellulose-based fuels and chemicals, including 1) integration of cellulase production systems into current cellulosic ethanol processes; 2) combination of processes and facilities between cellulosic ethanol and first-generation ethanol; 3) application of enzyme-free saccharification processes and computational ap-proaches to increase the bioethanol yield and optimize the integration process; 4) production of multiple products to maximize the value derived from the lignocellulosic biomass. Finally, the remaining challenges and perspectives of this field are also discussed. LA - English DB - MTMT ER - TY - JOUR AU - Stępień, Arkadiusz AU - Rejmer, Wojciech TI - Effect of Fertilization with Meat and Bone Meal on the Production of Biofuel Obtained from Corn Grain JF - ENERGIES J2 - ENERGIES VL - 16 PY - 2022 IS - 1 SP - 21 SN - 1996-1073 DO - 10.3390/en16010021 UR - https://m2.mtmt.hu/api/publication/33645608 ID - 33645608 AB - The large volumes of meat and bone meal (MBM) being produced are leading to an increased demand for research into innovative methods of utilizing MBM and obtaining further benefits. The object of this study is to analyze the efficiency of bioethanol and biodiesel production obtained from corn grain fertilized with meat and bone meal produced from animal waste. For the realization of this study, a four-year field experiment was carried out with grain corn fertilized with different doses of meat and bone meal in comparison to fertilization with mineral fertilizers and no fertilization. Fertilization with meat and bone meal should be considered not only for its direct effect but also for the after-effect. The effect of meat and bone meals on obtaining a grain yield higher than that obtained on objects without fertilization and those fertilized with mineral fertilizers was noticeable after applying higher doses from the third year of the study. Fertilization with meat and bone meals did not significantly affect the average fat content of grain, and it only slightly affected the starch content. The positive effect of meat and bone meals on the yield of bioethanol from grain extracted from one hectare was responsible for their yield-forming effect. The differences obtained between years and between fertilizer variants in the yields of ethyl biodiesel and methyl biodiesel per one hectare were mainly related to grain yields, rather than the obtained volume per 1 kg from grain. LA - English DB - MTMT ER - TY - JOUR AU - Barampouti, Elli Maria AU - Grammatikos, Christos AU - Stoumpou, Vasileia AU - Malamis, Dimitris AU - Mai, Sofia TI - Emerging Synergies on the Co-treatment of Spent Coffee Grounds and Brewer's Spent Grains for Ethanol Production JF - WASTE AND BIOMASS VALORIZATION J2 - WASTE BIOMASS VALORI PY - 2021 PG - 15 SN - 1877-2641 DO - 10.1007/s12649-021-01543-6 UR - https://m2.mtmt.hu/api/publication/32391955 ID - 32391955 AB - Purpose Given that the depletion of fossil fuels is an important issue that should be tackled urgently, alternative routes are being sought for biofuels production. This paper, under this context, studied the potential synergies of co-treatment of two kinds of agro-waste (brewers' spent grains, BSG and spent coffee grounds, SCG) towards the direction of biodiesel and bioethanol production, alleviating at the same time their treatment and management issues. Methods The technical feasibility of oil extraction and ethanol production was studied and optimized in both laboratory and bench scale. A treatment train including alkaline pretreatment of SCG and dilute acid pretreatment of BSG, neutralization of the solutions by mixing them, enzymatic saccharification and ethanolic fermentation led to the production of bioethanol. Results The co-treatment of BSG and SCG proved beneficial and optimum experimental conditions were defined by setting the bioethanol production cost as optimization parameter. The lowest ethanol production cost (3.9euro/kg) was observed for scenario 4 (SCG/BSG mixture, chemical pretreatment (0.5 N, 5 h), enzymatic hydrolysis (400 mu L/g cellulose, 16 h)) and scenario 5 (SCG/BSG mixture, chemical pretreatment (0.5 N, 5 h), enzymatic hydrolysis (800 mu L/g cellulose, 5 h)). Further upscaling of the process verified the technical feasibility of process units leading to a further increase of ethanol yield and thus a decrease in the production cost. Conclusions Conclusively, it was demonstrated that the recovery and extraction of the oil content of BSG and SCG and the achievement of high ethanol yields is technically feasible. Within this context, an integrated biorefinery was presented, making evident that these substrates could reverse the eminent energy crisis via biofuels production. Graphic Abstract LA - English DB - MTMT ER - TY - JOUR AU - Lamichhane, Ganesh AU - Khadka, Sujan AU - Acharya, Ashis AU - Parajuli, Niranjan TI - Pretreatment of finger millet straw (Eleusine coracana) for enzymatic hydrolysis towards bioethanol production JF - BIOMASS CONVERSION AND BIOREFINERY J2 - BIOMASS CONV BIOREFINERY PY - 2021 PG - 15 SN - 2190-6815 DO - 10.1007/s13399-021-01633-4 UR - https://m2.mtmt.hu/api/publication/32391956 ID - 32391956 AB - To obtain high yields of products through bioconversion processes, biomass must be pretreated to change its recalcitrant structures. The key goal of this analysis is to compare the findings in terms of total reducing sugar yield after organic solvents and hot water pretreatment on finger millet straw (FMS) using laboratory-scale equipment. Accordingly, the effect of two separate pretreatments, i.e., liquid hot water, and organic solvent (ethanol) at autoclave, and furnace temperatures, was investigated in this study to increase the enzymatic conversion of FMS under optimal conditions. The biomass recovery was found highest under the liquid hot water (autoclave; 9.477 +/- 0.017%) pretreatment conditions. In addition, the cellulose content improved significantly with pretreatment, increasing from 41.7 +/- 0.74 to 55.33 +/- 0.89%, while the hemicellulose and lignin contents significantly decreased. The untreated sample having a lignin amount of around 6.42 +/- 0.21% after different pretreatment methods dropped significantly (p = 0.00737**) and was found to have a 39.8 +/- 1.84% delignification rate based on the ethanol autoclave method. The findings of the study revealed that the FMS is severely affected by liquid hot water and organic solvent pretreatment in the autoclave than the furnace. After the pretreatment procedure, the maximum amount of crystalline cellulose in biomass was transformed to the amorphous state, as shown by the Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The pretreated cellulase blend enzyme sample was most active on the biomass treated with 1:20% (w/v) liquid hot water combined with autoclave heating at 121 degrees C, with 15 psi for 20 min, resulting in highest total reducing sugars; thus, results showed that the pretreated sample (0.75 g) contains 60.94 mg/g of fermentative sugars based on a 15% (w/v) ratio which claimed to be the fact that this is a better result than any other research by using FMS. LA - English DB - MTMT ER - TY - JOUR AU - Lamichhane, Ganesh AU - Acharya, Ashis AU - Poudel, Darbin Kumar AU - Aryal, Babita AU - Gyawali, Narayan AU - Niraula, Purushottam AU - Phuyal, Sita Ram AU - Budhathoki, Prakriti AU - Bk, Ganesh AU - Parajuli, Niranjan TI - Recent advances in bioethanol production from Lignocellulosic biomass JF - INTERNATIONAL JOURNAL OF GREEN ENERGY J2 - INT J GREEN ENERGY VL - 18 PY - 2021 IS - 7 SP - 731 EP - 744 PG - 14 SN - 1543-5075 DO - 10.1080/15435075.2021.1880910 UR - https://m2.mtmt.hu/api/publication/32391957 ID - 32391957 AB - The field of bioethanol production has seen rapid advancements in the past few decades. Despite the advancements, bioethanol production's recent trends cannot prove effective and efficient transportation fuel. Fossil fuels, which currently power-ups most energy demands, from nonrenewable energies, meaning it will bring adverse effects to the environment, including ozone layer depletion and global warming. It makes bioethanol the best alternative to fossil fuels, owing to the fact than it is considered carbon-neutral. In some parts of the world, bioethanol is utilized as an alternative fuel by using the lignocellulosic (LC) residues, and prioritizing its production as better feedstocks available could fulfill the void created in the transportation fuel sector. Separate hydrolysis and co-fermentation (SSCF) and Pre-hydrolysis and simultaneous saccharification and co-fermentation (PSSCF) are superior to all techniques to achieve saccharification, hexose, and pentose fermentation in a one-pot reaction. For the feedstock modification, the genome engineering and cell wall modification are advanced methods so far. As of not long ago, there is no cost-effective and financially achievable pretreatment technique for the removal of lignin that could be industrialized to satisfy the worldwide requirement for bioethanol. In this review we provide the present scenario of energy demand and advancement on bioethanol technology and challenges. Mainly, altering the LC biomass composition, the robust enzyme that enables to hydrolyzed and maximum fermentable sugar yield, and effectively fermenting bacteria and yeast, which is genetically engineered for simultaneous fermentation with the high rate, is future stepping research which should be emphasized in the bioethanol industry. LA - English DB - MTMT ER - TY - JOUR AU - Pilarski, Krzysztof AU - Pilarska, Agnieszka A. AU - Boniecki, Piotr AU - Niedbala, Gniewko AU - Witaszek, Kamil AU - Piekutowska, Magdalena AU - Idzior-Haufa, Malgorzata AU - Wawrzyniak, Agnieszka TI - Degree of Biomass Conversion in the Integrated Production of Bioethanol and Biogas JF - ENERGIES J2 - ENERGIES VL - 14 PY - 2021 IS - 22 PG - 16 SN - 1996-1073 DO - 10.3390/en14227763 UR - https://m2.mtmt.hu/api/publication/33003548 ID - 33003548 AB - The integrated production of bioethanol and biogas makes it possible to optimise the production of carriers from renewable raw materials. The installation analysed in this experimental paper was a hybrid system, in which waste from the production of bioethanol was used in a biogas plant with a capacity of 1 MWe. The main objective of this study was to determine the energy potential of biomass used for the production of bioethanol and biogas. Based on the results obtained, the conversion rate of the biomass-maize, in this case-into bioethanol was determined as the efficiency of the process of bioethanol production. A biomass conversion study was conducted for 12 months, during which both maize grains and stillage were sampled once per quarter (QU-I, QU-II, QU-III, QU-IV; QU-quarter) for testing. Between 342 L (QU-II) and 370 L (QU-I) of ethanol was obtained from the organic matter subjected to alcoholic fermentation. The mass that did not undergo conversion to bioethanol ranged from 269.04 kg to 309.50 kg, which represented 32.07% to 36.95% of the organic matter that was subjected to the process of bioethanol production. On that basis, it was concluded that only two-thirds of the organic matter was converted into bioethanol. The remaining part-post-production waste in the form of stillage-became a valuable raw material for the production of biogas, containing one-third of the biodegradable fraction. Under laboratory conditions, between 30.5 m(3) (QU-I) and 35.6 m(3) (QU-II) of biogas per 1 Mg of FM (FM-fresh matter) was obtained, while under operating conditions, between 29.2 m(3) (QU-I) and 33.2 m(3) (QU-II) of biogas was acquired from 1 Mg of FM. The Biochemical Methane Potential Correction Coefficient (BMPCC), which was calculated based on the authors' formula, ranged from 3.2% to 7.4% in the analysed biogas installation. LA - English DB - MTMT ER - TY - JOUR AU - Ayodele, Bamidele Victor AU - Alsaffar, May Ali AU - Mustapa, Siti Indati TI - An overview of integration opportunities for sustainable bioethanol production from first- and second-generation sugar-based feedstocks JF - JOURNAL OF CLEANER PRODUCTION J2 - J CLEAN PROD VL - 245 PY - 2020 PG - 20 SN - 0959-6526 DO - 10.1016/j.jclepro.2019.118857 UR - https://m2.mtmt.hu/api/publication/31059579 ID - 31059579 AB - The production of bioethanol from second generation feedstocks which are mainly lignocellulosic biomass provides the opportunities for a cleaner, and low carbon biofuel that can serve as an alternative to fossil fuel. However, one major constrain is the high cost of production due to expensive pre-treatment technologies. On the other hand, bioethanol production using first generation feedstock is a proven and well-established technology with high bioethanol productivity and yield, but the process is identified with the issues of food-to-fuel debate and high environmental impact from land use charge. Integrating second generation bioethanol production process with the well proven first generation bioethanol production facilities has the potential of harnessing the synergistic effect that could maximize the technical, economic and environmental benefits of the integrated first and second generation process. This study examined literature on studies related to first generation bioethanol production, second generation bioethanol production and the integrated first and second generation processes with the aim of identifying the extent of integration opportunities for first and second generation bioethanol production from different sugar-based feedstocks. The review put into consideration the different process scenarios available in terms of the technical, economic and environmental benefits of the bioethanol production processes. The benefits of the different integration scenarios for bioethanol production were considered in comparison with the standalone first generation and second generation bioethanol production scenarios. Based on the consideration of the different integration scenarios, some recommendations were proposed for attention in future research on the integration of first and second generation bioethanol production from sugar-based feedstocks. (C) 2019 Elsevier Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Bai, Ruxue AU - Wang, Wen AU - Yu, Qiang AU - Zhang, Qinghua AU - Kong, Xiaoying AU - Sun, Yongming AU - Zhuang, Xinshu AU - Wang, Zhongming AU - Yuan, Zhenhong TI - Predictive Modelling of Sugar Release from Blended Garden Wastes in a Microwave-Assisted Hot Water Process JF - WASTE AND BIOMASS VALORIZATION J2 - WASTE BIOMASS VALORI PY - 2020 PG - 10 SN - 1877-2641 DO - 10.1007/s12649-019-00932-2 UR - https://m2.mtmt.hu/api/publication/31701994 ID - 31701994 AB - Due to supply difficulties caused by seasonal availability, fuel ethanol production from a single lignocellulosic biomass species is always uneconomical at commercial scale. The utilization of blended biomass offers a potential solution to this problem. In this study, a prediction model was developed to evaluate the sugar release from blended garden wastes in a microwave-assisted hot water (MHW) process. The optimum blending ratio ofBauhinia blakeana Dunn(BB), rice straw (RS) and sugarcane bagasse (SC) was found to be 2:3:5, which promised a high total xylose yield of 67.82% (using a process temperature of 186 degrees C for 43 min). While the yield of xylose could only reach 52.47% when adopting the single garden waste of BB as feedstock under the same condition. Furthermore, the use of blended materials provided a cost savings of 22.23% as compared with the single RS feedstock, and no significant difference was found in the release of total xylose. The model developed was found to accurately predict the total xylose released from blended garden feedstocks. In addition, a steady supply of lignocellulosic biomass for fuel ethanol production could also be generated.[GRAPHICS]. LA - English DB - MTMT ER - TY - JOUR AU - Martin, Michael AU - Wetterlund, Elisabeth AU - Hackl, Roman AU - Holmgren, Kristina M. AU - Peck, Philip TI - Assessing the aggregated environmental benefits from by-product and utility synergies in the Swedish biofuel industry JF - BIOFUELS J2 - BIOFUELS-UK VL - 11 PY - 2020 IS - 6 SP - 683 EP - 698 PG - 16 SN - 1759-7269 DO - 10.1080/17597269.2017.1387752 UR - https://m2.mtmt.hu/api/publication/31701989 ID - 31701989 AB - The production of biofuels in Sweden has increased significantly in the past years in order to reduce fossil fuel dependence and mitigate climate impacts. Nonetheless, current methodological guidelines for assessing the GHG savings from the use of biofuels do not fully account for benefits from by-products and other utilities (e.g. waste heat and electricity) from biofuel production. This study therefore reviews the aggregated environmental performance of these multi-functional biofuel systems by assessing impacts and benefits from relevant production processes in Sweden in order to improve the decision base for biofuel producers and policymakers in the transition to a bio-based and circular economy. This was done by (1) conducting a mapping of the Swedish biofuel production portfolio, (2) developing future production scenarios, and (3) application of life cycle assessment methodology to assess the environmental performance of the production processes. Special focus was provided to review the potential benefits from replacing conventional products and services with by-products and utilities. The results provide evidence that failure to account for non-fuel-related benefits from biofuel production leads to an underestimation of the contribution of biofuels to reduce greenhouse gas emissions and other environmental impacts when replacing fossil fuels, showing the importance of their multi-functionality. LA - English DB - MTMT ER - TY - JOUR AU - Persson, Michael AU - Galbe, Mats AU - Wallberg, Ola TI - A strategy for synergistic ethanol yield and improved production predictability through blending feedstocks JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 13 PY - 2020 IS - 1 PG - 11 SN - 1754-6834 DO - 10.1186/s13068-020-01791-z UR - https://m2.mtmt.hu/api/publication/31701990 ID - 31701990 AB - Background The integration of first- and second-generation bioethanol processes has the potential to accelerate the establishment of second-generation bioethanol on the market. Cofermenting pretreated wheat straw with a glucose-rich process stream, such as wheat grain hydrolysate, in a simultaneous saccharification and fermentation process could address the technical issues faced during the biological conversion of lignocellulose to ethanol. For example, doing so can increase the final ethanol concentration in the broth and mitigate the effects of inhibitors formed during the pretreatment. Previous research has indicated that blends of first- and second-generation substrates during simultaneous saccharification and fermentation have synergistic effects on the final ethanol yield, an important parameter in the process economy. In this study, enzymatic hydrolysis and simultaneous saccharification and fermentation were examined using blends of pretreated wheat straw and saccharified wheat grain at various ratios. The aim of this study was to determine the underlying mechanisms of the synergy of blending with regard to the yield and volumetric productivity of ethanol. Results Replacing 25% of the pretreated wheat straw with wheat grain hydrolysate during simultaneous saccharification and fermentation was sufficient to decrease the residence time needed to deplete soluble glucose from 96 to 24 h and shift the rate-limiting step from ethanol production to the rate of enzymatic hydrolysis. Further, a synergistic effect on ethanol yield was observed with blended substrates, coinciding with lower glycerol production. Also, blending substrates had no effect on the yield of enzymatic hydrolysis. Conclusions The effects of substrate blending on the volumetric productivity of ethanol were attributed to changes in the relative rates of cell growth and cell death due to alterations in the concentrations of substrate and pretreatment-derived inhibitors. The synergistic effect of substrate blending on ethanol yield was attributed in part to the decreased production of cell mass and glycerol. Thus, it is preferable to perform simultaneous saccharification and fermentation with substrate blends rather than pure substrates with regard to yield, productivity, and the robustness of the process. LA - English DB - MTMT ER - TY - JOUR AU - Sanchis-Sebastia, Miguel AU - Erdei, Borbala AU - Kovacs, Krisztina AU - Galbe, Mats AU - Wallberg, Ola TI - Analysis of Animal Bedding Heterogeneity for Potential Use in Biorefineries Based on Farmyard Manure JF - WASTE AND BIOMASS VALORIZATION J2 - WASTE BIOMASS VALORI VL - 11 PY - 2020 IS - 6 SP - 2387 EP - 2395 PG - 9 SN - 1877-2641 DO - 10.1007/s12649-018-00578-6 UR - https://m2.mtmt.hu/api/publication/31448480 ID - 31448480 AB - Purpose Animal bedding, a mixture of straw and manure, could be used as a feedstock for bioenergy production, but its heterogeneity poses new challenges in its use in biorefineries. We have investigated the origin of this heterogeneity and quantified it, and discuss its impact on bioenergy production. Methods Samples were collected from the bedding at different points and depths and analysed by first separating the manure from the straw by means of washing with water, and then determining the chemical composition of the liquid and solid fractions. Results The results supported our hypothesis that animal bedding behaves as a combination of several layers at different stages of degradation. Analysis revealed that the layers with higher organic content in the manure exhibited a poorer performance during the washing, since the residence time in the barn alters the washing profile of the organic fraction in the manure. It was also found that the variability in the composition of animal bedding was much greater than in other agricultural feedstocks: the manure content in animal bedding varied from 26 to 41%, and the content of fermentable carbohydrates varied by 20%. Total carbon and total nitrogen analyses showed that these changes in composition also affected the C/N ratio of the material, and thus its suitability as a feedstock for anaerobic digestion. Conclusions This implies that the residence time in the barn affects not only the heterogeneity of the properties of animal bedding, but also the best way to process it in a biorefinery. LA - English DB - MTMT ER - TY - JOUR AU - Sharma, Bhavna AU - Brandt, Craig AU - McCullough-Amal, Devita AU - Langholtz, Matthew AU - Webb, Erin TI - Assessment of the feedstock supply for siting single- and multiple-feedstock biorefineries in the USA and identification of prevalent feedstocks JF - BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR J2 - BIOFUEL BIOPROD BIOR VL - 14 PY - 2020 IS - 3 SP - 578 EP - 593 PG - 16 SN - 1932-104X DO - 10.1002/bbb.2091 UR - https://m2.mtmt.hu/api/publication/31448479 ID - 31448479 AB - An integrated multi-feedstock bioenergy (i.e., biofuel, biopower, or bioproduct) supply system has potential to reduce biomass supply system uncertainties and costs. This study identifies optimal configurations of multi-feedstock biomass-to-biorefinery supply chains and pertinent feedstock combinations based on spatial distribution of feedstock and lowest delivered cost to the biorefinery. We used the Supply Characterization Model (SCM) to allocate feedstock supplies to candidate biorefinery facilities. Model runs were performed for herbaceous energy crops, agriculture residue, and woody biomass available in 2017, 2022, 2025, and 2030 as estimated by the Policy Analysis System (POLYSYS) and Forest Sustainable and Economic Analysis Model (ForSEAM) models. Three feedstock supply scenarios were compared: (a) an herbaceous scenario: switchgrass, miscanthus, biosorghum, and corn stover; (b) a woody scenario: coppice wood, noncoppice wood, whole trees, and forestry residues, and (c) a mixed scenario: a combination of all feedstocks in herbaceous and woody scenarios. By 2030 the analyses predicted that 323, 168, and 473 biorefineries were sited in the herbaceous, woody, and mixed scenario, respectively, in the conterminous USA. Feedstock mixes supplied to the biorefineries were mostly dominated by a single feedstock. The most prominent feedstock mixes identified were: (1) switchgrass and miscanthus; (2) coppice and noncoppice wood; and (3) coppice wood, noncoppice wood, switchgrass and miscanthus. Biorefineries using multi-feedstock would be beneficial for growth of bioeconomy, however flexible and cost-effective conversion platforms should be developed to efficiently utilize multiple feedstocks. This analysis identifies biorefinery locations and feedstock supply mixes while minimizing delivered feedstock costs based on spatial and temporal feedstock availability. (c) 2020 Society of Chemical Industry and John Wiley & Sons, Ltd LA - English DB - MTMT ER - TY - JOUR AU - Sharma, Shalley AU - Arora, Anju TI - Tracking strategic developments for conferring xylose utilization/fermentation bySaccharomyces cerevisiae JF - ANNALS OF MICROBIOLOGY J2 - ANN MICROBIOL VL - 70 PY - 2020 IS - 1 PG - 17 SN - 1590-4261 DO - 10.1186/s13213-020-01590-9 UR - https://m2.mtmt.hu/api/publication/31448478 ID - 31448478 AB - Purpose Efficient ethanol production through lignocellulosic biomass hydrolysates could solve energy crisis as it is economically sustainable and ecofriendly.Saccharomyces cerevisiaeis the work horse for lignocellulosic bioethanol production at industrial level. But its inability to ferment and utilize xylose limits the overall efficacy of the process. Method Data for the review was selected using different sources, such as Biofuels digest, Statista, International energy agency (IEA). Google scholar was used as a search engine to search literature for yeast metabolic engineering approaches. Keywords used were metabolic engineering of yeast for bioethanol production from lignocellulosic biomass. Result Through these approaches, interconnected pathways can be targeted randomly. Moreover, the improved strains genetic makeup can help us understand the mechanisms involved for this purpose. Conclusion This review discusses all possible approaches for metabolic engineering of yeast. These approaches may reveal unknown hidden mechanisms and construct ways for the researchers to produce novel and modified strains. LA - English DB - MTMT ER - TY - JOUR AU - Suhag, Meenakshi AU - Kumar, Anil AU - Singh, Joginder TI - Saccharification and fermentation of pretreated banana leaf waste for ethanol production JF - SN APPLIED SCIENCES J2 - SN APPL SCI VL - 2 PY - 2020 IS - 8 PG - 9 SN - 2523-3963 DO - 10.1007/s42452-020-03215-x UR - https://m2.mtmt.hu/api/publication/31423583 ID - 31423583 N1 - Cited By :7 Export Date: 14 October 2022 Correspondence Address: Singh, J.; Laboratory of Environmental Biotechnology, India; email: jssdahiya@gmail.com AB - The present work reveals the potential applicability of banana leaf waste as feedstock for bioethanol production as very less work has been reported on this feedstock. For achieving the same, the performance of crude cellulases of Aspergillus niger JD-11 is explored for saccharification of untreated and pretreated (alkaline, dilute acid and steam) banana leaf waste. Therefore, to determine the best saccharifying conditions, the effects of various parameters such as enzyme loading (5-15 FPU/g), temperature (40-50 degrees C), surfactant addition (0.05-0.15% Tween 80 and PEG 6000) and substrate concentrations (2-6%) were studied at 150 rpm and pH 5.0. The crude cellulases yielded maximum reducing sugars (524.83 mg/g) from the dilute acid pretreated banana leaf waste at 45 degrees C, 15 FPU/g enzyme loading and 2% substrate loading in the presence of 0.15% PEG 6000 (w/v) as surfactant. The fermentation of enzymatic hydrolysate was carried out using initial reducing sugars concentration of 40 g/L in the medium at pH 5.5, 150 rpm and 30 degrees C for 30 h with the help of Saccharomyces cerevisiae. Ethanol production was estimated at every 6 h and maximum ethanol yield of 0.38 g/g sugar and productivity of 1.28 g/L/h was obtained after 12 h of fermentation of banana leaf waste hydrolysate. Thus, the easily obtained banana leaf waste could be a promising feedstock for bioethanol production. LA - English DB - MTMT ER - TY - JOUR AU - Tang, Shizhe AU - Xu, Tingliang AU - Peng, Jing AU - Zhou, Kaiyan AU - Zhu, Yuling AU - Zhou, Wenbo AU - Cheng, Haina AU - Zhou, Hongbo TI - Overexpression of an endogenous raw starch digesting mesophilic alpha-amylase gene in Bacillus amyloliquefaciens Z3 by in vitro methylation protocol JF - JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE J2 - J SCI FOOD AGR VL - 100 PY - 2020 IS - 7 SP - 3013 EP - 3023 PG - 11 SN - 0022-5142 DO - 10.1002/jsfa.10332 UR - https://m2.mtmt.hu/api/publication/31448477 ID - 31448477 AB - BACKGROUND Mesophilic alpha-amylases function effectively at low temperatures with high rates of catalysis and require less energy for starch hydrolysis. Bacillus amyloliquefaciens is an essential producer of mesophilic alpha-amylases. However, because of the existence of the restriction-modification system, introducing exogenous DNAs into wild-type B. amyloliquefaciens is especially tricky.RESULTS alpha-Amylase producer B. amyloliquefaciens strain Z3 was screened and used as host for endogenous alpha-amylase gene expression. In vitro methylation was performed in recombinant plasmid pWB980-amyZ3. With the in vitro methylation, the transformation efficiency was increased to 0.96 x 10(2) colony-forming units mu g(-1) plasmid DNA. A positive transformant BAZ3-16 with the highest alpha-amylase secreting capacity was chosen for further experiments. The alpha-amylase activity of strain BAZ3-16 reached 288.70 +/- 16.15 U mL(-1) in the flask and 386.03 +/- 16.25 U mL(-1) in the 5-L stirred-tank fermenter, respectively. The Bacillus amyloliquefaciens Z3 expression system shows excellent genetic stability and high-level extracellular production of the target protein. Moreover, the synergistic interaction of AmyZ3 with amyloglucosidase was determined during the hydrolysis of raw starch. The hydrolysis degree reached 92.34 +/- 3.41% for 100 g L-1 raw corn starch and 81.30 +/- 2.92% for 100 g L-1 raw cassava starch after 24 h, respectively.CONCLUSION Methylation of the plasmid DNA removes a substantial barrier for transformation of B. amyloliquefaciens strain Z3. Furthermore, the exceptional ability to hydrolyze starch makes alpha-amylase AmyZ3 and strain BAZ3-16 valuable in the starch industry. (c) 2020 Society of Chemical Industry LA - English DB - MTMT ER - TY - JOUR AU - Ursachi, Vasile-Florin AU - Gutt, Gheorghe TI - Production of Cellulosic Ethanol from Enzymatically Hydrolysed Wheat Straws JF - APPLIED SCIENCES-BASEL J2 - APPL SCI-BASEL VL - 10 PY - 2020 IS - 21 PG - 22 SN - 2076-3417 DO - 10.3390/app10217638 UR - https://m2.mtmt.hu/api/publication/31701992 ID - 31701992 AB - The aim of this study is to find the optimal pretreatment conditions and hydrolysis in order to obtain a high yield of bioethanol from wheat straw. The pretreatments were performed with different concentrations of sulphuric acid 1, 2 and 3% (v/v), and were followed by an enzymatic hydrolysis that was performed by varying the solid-to-liquid ratio (1/20, 1/25 and 1/30 g/mL) and the enzyme dose (30/30 mu L/g, 60/60 mu L/g and 90/90 mu L/g Viscozyme(R) L/Celluclast(R) 1.5 L). This mix of enzymes was used for the first time in the hydrolysis process of wheat straws which was previously pretreated with dilute sulfuric acid. Scanning electron microscopy indicated significant differences in the structural composition of the samples because of the pretreatment with H2SO4 at different concentrations, and ATR-FTIR analysis highlighted the changes in the chemical composition in the pretreated wheat straw as compared to the untreated one. HPLC-RID was used to identify and quantify the carbohydrates content resulted from enzymatic hydrolysis to evaluate the potential of using wheat straws as a raw material for production of cellulosic ethanol in Romania. The highest degradation of lignocellulosic material was obtained in the case of pretreatment with 3% H2SO4 (v/v), a solid-to-liquid ratio of 1/30 and an enzyme dose of 90/90 mu L/g. Simultaneous saccharification and fermentation were performed using Saccharomyces cerevisiae yeast, and for monitoring the fermentation process a BlueSens equipment was used provided with ethanol, O-2 and CO2 cap sensors mounted on the fermentation flasks. The highest concentration of bioethanol was obtained after 48 h of fermentation and it reached 1.20% (v/v). LA - English DB - MTMT ER - TY - JOUR AU - Yang, Qing AU - Yang, Yongfu AU - Tang, Ying AU - Wang, Xia AU - Chen, Yunhao AU - Shen, Wei AU - Zhan, Yangyang AU - Gao, Junjie AU - Wu, Bo AU - He, Mingxiong AU - Chen, Shouwen AU - Yang, Shihui TI - Development and characterization of acidic-pH-tolerant mutants of Zymomonas mobilis through adaptation and next-generation sequencing-based genome resequencing and RNA-Seq JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 13 PY - 2020 IS - 1 PG - 17 SN - 1754-6834 DO - 10.1186/s13068-020-01781-1 UR - https://m2.mtmt.hu/api/publication/31701995 ID - 31701995 AB - Background: Acid pretreatment is a common strategy used to break down the hemicellulose component of the lignocellulosic biomass to release pentoses, and a subsequent enzymatic hydrolysis step is usually applied to release hexoses from the cellulose. The hydrolysate after pretreatment and enzymatic hydrolysis containing both hexoses and pentoses can then be used as substrates for biochemical production. However, the acid-pretreated liquor can also be directly used as the substrate for microbial fermentation, which has an acidic pH and contains inhibitory compounds generated during pretreatment. Although the natural ethanologenic bacteriumZymomonas mobiliscan grow in a broad range of pH 3.5 similar to 7.5, cell growth and ethanol fermentation are still affected under acidic-pH conditions below pH 4.0.Results: In this study, adaptive laboratory evolution (ALE) strategy was applied to adaptZ. mobilisunder acidic-pH conditions. Two mutant strains named 3.6M and 3.5M with enhanced acidic pH tolerance were selected and confirmed, of which 3.5M grew better than ZM4 but worse than 3.6M in acidic-pH conditions that is served as a reference strain between 3.6M and ZM4 to help unravel the acidic-pH tolerance mechanism. Mutant strains 3.5M and 3.6M exhibited 50 similar to 130% enhancement on growth rate, 4 similar to 9 h reduction on fermentation time to consume glucose, and 20 similar to 63% improvement on ethanol productivity than wild-type ZM4 at pH 3.8. Next-generation sequencing (NGS)-based whole-genome resequencing (WGR) and RNA-Seq technologies were applied to unravel the acidic-pH tolerance mechanism of mutant strains. WGR result indicated that compared to wild-type ZM4, 3.5M and 3.6M have seven and five single nucleotide polymorphisms (SNPs), respectively, among which four are shared in common. Additionally, RNA-Seq result showed that the upregulation of genes involved in glycolysis and the downregulation of flagellar and mobility related genes would help generate and redistribute cellular energy to resist acidic pH while keeping normal biological processes inZ. mobilis. Moreover, genes involved in RND efflux pump, ATP-binding cassette (ABC) transporter, proton consumption, and alkaline metabolite production were significantly upregulated in mutants under the acidic-pH condition compared with ZM4, which could help maintain the pH homeostasis in mutant strains for acidic-pH resistance. Furthermore, our results demonstrated that in mutant 3.6M, genes encoding F(1)F(0)ATPase to pump excess protons out of cells were upregulated under pH 3.8 compared to pH 6.2. This difference might help mutant 3.6M manage acidic conditions better than ZM4 and 3.5M. A few gene targets were then selected for genetics study to explore their role in acidic pH tolerance, and our results demonstrated that the expression of two operons in the shuttle plasmids,ZMO0956-ZMO0958encoding cytochrome bc1 complex andZMO1428-ZMO1432encoding RND efflux pump, could helpZ. mobilistolerate acidic-pH conditions.Conclusion: An acidic-pH-tolerant mutant 3.6M obtained through this study can be used for commercial bioethanol production under acidic fermentation conditions. In addition, the molecular mechanism of acidic pH tolerance ofZ. mobiliswas further proposed, which can facilitate future research on rational design of synthetic microorganisms with enhanced tolerance against acidic-pH conditions. Moreover, the strategy developed in this study combining approaches of ALE, genome resequencing, RNA-Seq, and classical genetics study for mutant evolution and characterization can be applied in other industrial microorganisms. LA - English DB - MTMT ER - TY - JOUR AU - Yin, Yi-Rui AU - Li, Tao AU - Sang, Peng AU - Yang, Run-Fen AU - Liu, Hong-Yan AU - Xiao, Min AU - Li, Shuai AU - Yang, Li-Quan AU - Li, Wen-Jun TI - Characterization of an alkali-tolerant, thermostable, and multifunctional GH5 family endoglucanase fromThermoactinospora rubraYIM 77501(T)for prebiotic production JF - BIOMASS CONVERSION AND BIOREFINERY J2 - BIOMASS CONV BIOREFINERY PY - 2020 PG - 10 SN - 2190-6815 DO - 10.1007/s13399-020-00848-1 UR - https://m2.mtmt.hu/api/publication/31450033 ID - 31450033 AB - A novel endoglucanase gene (1425 bp), designatedthrcel5A, was cloned fromThermoactinospora rubraYIM 77501(T)and determined to be a member of glycoside hydrolase family 5. The putative amino acid sequence displayed 76% conservation with reported endoglucanases (GenBank: SCG57304.1) fromMicromonospora siamensis.Thrcel5Awas expressed inEscherichia coliBL 21 (DE3) and purified using Ni2+-affinity chromatography. The resulting purified protein displayed high hydrolytic activity against the sodium salt of carboxymethyl cellulose and beta-(1, 3; 1, 4)-glucans from barley and beechwood xylan, with specific activities of 85.7 +/- 1.5, 120.3 +/- 2.6, and 22.9 +/- 1.1 U/mg, respectively. The optimal pH and temperature for the recombinant enzyme were determined to be 8.5 and 60 degrees C, respectively. Additionally, ThrCel5A was thermotolerant as it retained more than 60% of its original activity after an incubation at 60 degrees C for 2 h. Moreover, ThrCel5A can hydrolyze beta-(1, 3; 1, 4)-glucan into prebiotics, such as cellobiose, cellotriose, and cellotetrose. Its endoglucanase activity was significantly affected by link sequences and CBM2. Due to being an alkali-tolerant, thermostable, and multifunctional cellulolytic enzyme, ThrCel5A is an attractive candidate for use in production of prebiotics. LA - English DB - MTMT ER - TY - JOUR AU - El-Imam, Amina M. Ahmed AU - Greetham, Darren AU - Du, Chenyu AU - Dyer, Paul S. TI - The development of a biorefining strategy for the production of biofuel from sorghum milling waste JF - BIOCHEMICAL ENGINEERING JOURNAL J2 - BIOCHEM ENG J VL - 150 PY - 2019 PG - 11 SN - 1369-703X DO - 10.1016/j.bej.2019.107288 UR - https://m2.mtmt.hu/api/publication/31573268 ID - 31573268 AB - The potential of using sorghum milling waste for the development of a biorefining strategy for the production of bioethanol was investigated. Both red and white sorghum were processed using a traditional Nigerian wetmilling process to sorghum flour. The sorghum milling waste, sorghum bran, was hydrolysed using both enzymatic and dilute acid hydrolysis to produce a generable fermentation feedstock. The hydrolysates were subsequently investigated for fermentative biofuel production. Following a hydrolysis step, a medium containing (similar to)61 g/L glucose was obtained. Trace presence of inhibitors was detected in the hydrolysates and sufficient nitrogen content to support microorganism growth and bioethanol production. In test bioethanol production experiments using the sorghum milling waste derived hydrolysates only, 24.35 g/L bioethanol was produced by a yeast Kluyveromyces marxianus, equivalent to a yield of 0.15 g bioethanol per g of sorghum milling waste. LA - English DB - MTMT ER - TY - JOUR AU - Fan, Meishan AU - Li, Jun AU - Bi, Guican AU - Ye, Guangying AU - Zhang, Hongdan AU - Xie, Jun TI - Enhanced co-generation of cellulosic ethanol and methane with the starch/sugar-rich waste mixtures and Tween 80 in fed-batch mode JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 12 PY - 2019 IS - 1 PG - 12 SN - 1754-6834 DO - 10.1186/s13068-019-1562-0 UR - https://m2.mtmt.hu/api/publication/31058008 ID - 31058008 AB - Background The mixed-feedstock fermentation is a promising approach to enhancing the co-generation of cellulosic ethanol and methane from sugarcane bagasse (SCB) and molasses. However, the unmatched supply of the SCB and molasses remains a main obstacle built upon binary feedstock. Here, we propose a cellulose-starch-sugar ternary waste combinatory approach to overcome this bottleneck by integrating the starch-rich waste of Dioscorea composita Hemls. extracted residue (DER) in mixed fermentation. Results The substrates of the pretreated SCB, DER and molasses with varying ratios were conducted at a relatively low solids loading of 12%, and the optimal mixture ratio of 1:0.5:0.5 for the pretreated SCB/DER/molasses was determined by evaluating the ethanol concentration and yield. Nevertheless, it was found that the ethanol yield decreased from 79.19 +/- 0.20 to 62.31 +/- 0.61% when the solids loading increased from 12 to 44% in batch modes, regardless of the fact that the co-fermentation of three-component feedstock was performed under the optimal condition defined above. Hence, different fermentation processes such as fed-batch and fed-batch + Tween 80 were implemented to further improve the ethanol concentration and yield at higher solids loading ranging between 36 and 44%. The highest ethanol concentration of 91.82 +/- 0.86 g/L (69.33 +/- 0.46% of theoretical yield) was obtained with fed-batch + Tween 80 mode during the simultaneous saccharification and fermentation at a high solids loading of 44%. Moreover, after the ethanol recovery, the remaining stillage was digested for biomethane production and finally yielded 320.72 +/- 6.98 mL/g of volatile solids. Conclusions Integrated DER into the combination of SCB and molasses would be beneficial for ethanol production. The co-generation of bioethanol and biomethane by mixed cellulose-starch-sugar waste turns out to be a sustainable solution to improve the overall efficacy in biorefinery. LA - English DB - MTMT ER - TY - JOUR AU - Jamaldheen, Sumitha Banu AU - Thakur, Abhijeet AU - Moholkar, Vijayanand S. AU - Goyal, Arun TI - Enzymatic hydrolysis of hemicellulose from pretreated Finger millet Check for (Eleusine coracana) straw by recombinant endo-1,4-beta-xylanase updates and exo-1.4-beta-xylosidase JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 135 PY - 2019 SP - 1098 EP - 1106 PG - 9 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2019.06.010 UR - https://m2.mtmt.hu/api/publication/31059584 ID - 31059584 AB - This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose. The variation in the carbohydrate composition of FMS was analysed when subjected to different pretreatments. The recombinant endo-1.4-beta-xylanase (CtXynl 1A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 degrees C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass. The pretreatment aided in concentrating the holocellulose content from 69.3% of raw powdered FMS to 76.4%. The post-treatment solid biomass yield was 0.36 g/g raw biomass. The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo1.4-beta-xylanase (CtXyn11A) at 55 degrees C and ii) exo-1.4-beta-xylosidase (BoGH43A) at 37 degrees C, both at pH 7.5 by BoxBehnken design yielded the TRS of 70 mg/g pretreated biomass. The percentage conversion of xylan to xylose by CtXynl IA and BoGH43A was 24.7%. (C) 2019 Elsevier B.V. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Priharto, Neil AU - Ronsse, Frederik AU - Prins, Wolter AU - Hita, Idoia AU - Deuss, Peter J. AU - Heeres, Hero Jan TI - Hydrotreatment of pyrolysis liquids derived from second-generation bioethanol production residues over NiMo and CoMo catalysts JF - BIOMASS & BIOENERGY J2 - BIOMASS BIOENERGY VL - 126 PY - 2019 SP - 84 EP - 93 PG - 10 SN - 0961-9534 DO - 10.1016/j.biombioe.2019.05.005 UR - https://m2.mtmt.hu/api/publication/31059580 ID - 31059580 AB - Lignin-rich digested stillage from second-generation bioethanol production is a unique biomass-derived feedstock, not only because it contains high amounts of lignin but also due to its residual amounts of cellulose and hemicellulose. In this study, catalytic hydrotreatment experiments were conducted on pyrolysis liquids obtained from the lignin-rich feedstock using sulphided NiMo/Al2O3 and CoMo/Al2O3 catalysts. The aim was to obtain a high conversion of the initial pyrolysis feed into a hydrotreated oil with a high phenolics and aromatics fractions. Experiments were carried out in a stirred batch reactor at 350 degrees C and 10 MPa of H-2 (initial pressure). Product oils were obtained in about 60-65% w/w, the remainder being an aqueous phase (12-14% w/w), solids (7-8% w/w) and gas phase components (all on initial pyrolysis oil feed basis). The product oils were characterised in detail using various techniques (elemental composition, GCxGC-FID, GPC, and 2D HSQC NMR). The oxygen content was reduced from 23% w/w in the pyrolysis oils to 7.5-11.5% in the hydrotreated oils, indicative of the occurrence of hydrodeoxygenation reactions. This was also evident from the chemical composition, showing an increase in the amounts of low molecular weight aromatics, alkylphenolics, alkanes and cycloalkanes in hydrotreated oils. Performance of the two catalysts was compared, and a higher degree of deoxygenation was observed for the NiMo catalyst. The implications of the findings for the valorisation of second-generation bioethanol residues are also discussed. LA - English DB - MTMT ER - TY - JOUR AU - Sanchis-Sebastia, Miguel AU - Erdei, Borbala AU - Kovacs, Krisztina AU - Galbe, Mats AU - WallbergE, Ola TI - Introducing low-quality feedstocks in bioethanol production: efficient conversion of the lignocellulose fraction of animal bedding through steam pretreatment JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 12 PY - 2019 IS - 1 PG - 10 SN - 1754-6834 DO - 10.1186/s13068-019-1558-9 UR - https://m2.mtmt.hu/api/publication/31059583 ID - 31059583 AB - Background Animal bedding remains an underutilized source of raw material for bioethanol production, despite the economic and environmental benefits of its use. Further research concerning the optimization of the production process is needed, as previously tested pretreatment methods have not increased the conversion efficiency to the levels necessary for commercialization of the process. Results We propose steam pretreatment of animal bedding, consisting of a mixture of straw and cow manure, to deliver higher ethanol yields. The temperature, residence time and pH were optimized through response-surface modeling, where pretreatment was evaluated based on the ethanol yield obtained through simultaneous saccharification and fermentation of the whole pretreated slurry. The results show that the best conditions for steam pretreatment are 200 degrees C, for 5 min at pH 2, at which an ethanol yield of about 70% was obtained. Moreover, the model also showed that the pH had the greatest influence on the ethanol yield, followed by the temperature and then the residence time. Conclusions Based on these results, it appears that steam pretreatment could unlock the potential of animal bedding, as the same conversion efficiencies were achieved as for higher-quality feedstocks such as wheat straw. LA - English DB - MTMT ER - TY - JOUR AU - Yu, Jianming AU - Xu, Zhaoxian AU - Liu, Lei AU - Chen, Sitong AU - Wang, Shengwei AU - Jin, Mingjie TI - Process integration for ethanol production from corn and corn stover as mixed substrates JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 279 PY - 2019 SP - 10 EP - 16 PG - 7 SN - 0960-8524 DO - 10.1016/j.biortech.2019.01.112 UR - https://m2.mtmt.hu/api/publication/30565436 ID - 30565436 AB - This work investigated all possible process integration strategies for ethanol production from corn and dilute acid pretreated corn stover (CS) as mixed substrates. Three corn to pretreated CS ratios (20%: 10%, 10%: 20% and 5%: 25%) were examined. When the ratio of corn to pretreated CS was 20%: 10%, the process integration strategy that mixed corn with CS hydrolysate for liquefaction followed by SSF resulted in the highest ethanol titer of 99.3 g/L. Mixing liquefied corn with pretreated CS for hydrolysis/saccharification followed by fermentation was the best strategy for the other two ratios. The strategy of mixing liquefied corn with pretreated CS for 6 h hydrolysis followed by fermentation showed the highest productivity for all the tested ratios. LA - English DB - MTMT ER - TY - JOUR AU - Buck, Michael AU - Senn, Thomas TI - Crop diversity for mixed first and second generation ethanol production JF - BIOFUELS J2 - BIOFUELS-UK VL - 9 PY - 2018 IS - 3 SP - 291 EP - 303 PG - 13 SN - 1759-7269 DO - 10.1080/17597269.2016.1266233 UR - https://m2.mtmt.hu/api/publication/27570770 ID - 27570770 LA - English DB - MTMT ER - TY - JOUR AU - Chen, Sitong AU - Xu, Zhaoxian AU - Li, Xiujuan AU - Yu, Jianming AU - Cai, Mufeng AU - Jin, Mingjie TI - Integrated bioethanol production from mixtures of corn and corn stover JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 258 PY - 2018 SP - 18 EP - 25 PG - 8 SN - 0960-8524 DO - 10.1016/j.biortech.2018.02.125 UR - https://m2.mtmt.hu/api/publication/27570762 ID - 27570762 LA - English DB - MTMT ER - TY - JOUR AU - Darwin, null AU - Ilham, Muhammad AU - Fazil, Afrizal TI - PERFORMANCE AND KINETIC STUDY OF THE ANAEROBIC CO-DIGESTION OF COCOA HUSK AND DIGESTED COW MANURE WITH HIGH ORGANIC LOADING RATE JF - INMATEH - AGRICULTURAL ENGINEERING J2 - INMATEH AGRICULT ENG VL - 55 PY - 2018 IS - 2 SP - 131 EP - 140 PG - 10 SN - 2068-2239 UR - https://m2.mtmt.hu/api/publication/30565440 ID - 30565440 AB - Biogas is a clean and cheap renewable energy that can be used for generating electricity. One of the current methods applied to enhance biogas production was through anaerobic co-digestion. The current study revealed that anaerobic digestion of cow manure co-digested with cocoa husk produced higher biogas production (348.3 mL/day) than anaerobic digestion of cow manure alone (26.5 mL/day). Even if a high organic loading rate (OLR) (4.173 kg VS/m(3).day) was applied to the reactor of anaerobic co-digestion, no inhibition was found at which pH was stable at 7.08. The results suggested that high OLR and short HRT (10 days) did not inhibit biogas production. LA - English DB - MTMT ER - TY - JOUR AU - Fan, Meishan AU - Zhang, Shuaishuai AU - Ye, Guangying AU - Zhang, Hongdan AU - Xie, Jun TI - Integrating sugarcane molasses into sequential cellulosic biofuel production based on SSF process of high solid loading JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 11 PY - 2018 PG - 9 SN - 1754-6834 DO - 10.1186/s13068-018-1328-0 UR - https://m2.mtmt.hu/api/publication/30565439 ID - 30565439 AB - BackgroundSugarcane bagasse (SCB) is one of the most promising lignocellulosic biomasses for use in the production of biofuels. However, bioethanol production from pure SCB fermentation is still limited by its high process cost and low fermentation efficiency. Sugarcane molasses, as a carbohydrate-rich biomass, can provide fermentable sugars for ethanol production. Herein, to reduce high processing costs, molasses was integrated into lignocellulosic ethanol production in batch modes to improve the fermentation system and to boost the final ethanol concentration and yield.ResultsThe co-fermentation of pretreated SCB and molasses at ratios of 3:1 (mixture A) and 1:1 (mixture B) were conducted at solid loadings of 12% to 32%, and the fermentation of pretreated SCB alone at the same solid loading was also compared. At a solid loading of 32%, the ethanol concentrations of 64.10g/L, 74.69g/L, and 75.64g/L were obtained from pure SCB, mixture A, and mixture B, respectively. To further boost the ethanol concentration, the fermentation of mixture B (1:1), with higher solid loading from 36 to 48%, was also implemented. The highest ethanol concentration of 94.20g/L was generated at a high solid loading of 44%, with an ethanol yield of 72.37%. In addition, after evaporation, the wastewater could be converted to biogas by anaerobic digestion. The final methane production of 312.14mL/g volatile solids (VS) was obtained, and the final chemical oxygen demand removal and VS degradation efficiency was 85.9% and 95.9%, respectively.ConclusionsMolasses could provide a good environment for the growth of yeast and inoculum. Integrating sugarcane molasses into sequential cellulosic biofuel production could improve the utilization of biomass resources. LA - English DB - MTMT ER - TY - JOUR AU - Ferreira, Jorge A. AU - Brancoli, Pedro AU - Agnihotri, Swarnima AU - Bolton, Kim AU - Taherzadeh, Mohammad J. TI - A review of integration strategies of lignocelluloses and other wastes in 1st generation bioethanol processes JF - PROCESS BIOCHEMISTRY J2 - PROCESS BIOCHEM VL - 75 PY - 2018 SP - 173 EP - 186 PG - 14 SN - 1359-5113 DO - 10.1016/j.procbio.2018.09.006 UR - https://m2.mtmt.hu/api/publication/30565438 ID - 30565438 AB - First-generation ethanol plants offer successful, commercial-scale bioprocesses that can, at least partially, replace fossil fuels. They can act as platforms to integrate lignocelluloses, wastes and residuals when establishing 2nd generation ethanol. The present review gathers recent insights on the integration of intrinsic and extrinsic substrates into lot generation ethanol plants, through microbial conversion or cogeneration systems. It shows that, among different lot generation ethanol plants, sugar-based ethanol by-products, dominate integration studies characterized by strong techno-economic and life-cycle assessment components. In comparison, there are fewer studies that focus on grain-derived lignocellulosic residuals and other wastes. There is consensus that integrating second generation feedstocks into first generation plants can have positive techno-economic and environmental impacts. In addition to realizing production of ethanol from 2nd generation feedstocks, these possibilities can impact waste management by establishing relevant biorefineries and circular economy. They can also supply a wide range of renewable products. Considering the potential of this waste management strategy, further research on these and many other substrates is needed. This will shed light on the effect of the integration, the relevant types of microorganisms and pretreatments, and of other physical parameters on the effectiveness of running lot generation plants with integrated second generation feedstocks. LA - English DB - MTMT ER - TY - JOUR AU - Li, Shi AU - Deng, Weiping AU - Wang, Shanshan AU - Wang, Pan AU - An, Dongli AU - Li, Yanyun AU - Zhang, Qinghong AU - Wang, Ye TI - Catalytic Transformation of Cellulose and Its Derivatives into Functionalized Organic Acids JF - CHEMSUSCHEM J2 - CHEMSUSCHEM VL - 11 PY - 2018 IS - 13 SP - 1995 EP - 2028 PG - 34 SN - 1864-5631 DO - 10.1002/cssc.201800440 UR - https://m2.mtmt.hu/api/publication/27593662 ID - 27593662 LA - English DB - MTMT ER - TY - JOUR AU - Mupondwa, Edmund AU - Li, Xue AU - Tabil, Lope TI - Integrated bioethanol production from triticale grain and lignocellulosic straw in Western Canada JF - INDUSTRIAL CROPS AND PRODUCTS J2 - IND CROP PROD VL - 117 PY - 2018 SP - 75 EP - 87 PG - 13 SN - 0926-6690 DO - 10.1016/j.indcrop.2018.02.070 UR - https://m2.mtmt.hu/api/publication/27570761 ID - 27570761 LA - English DB - MTMT ER - TY - JOUR AU - Sivamani, Selvaraju AU - Baskar, Rajoo TI - Process design and optimization of bioethanol production from cassava bagasse using statistical design and genetic algorithm JF - PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY J2 - PREP BIOCHEM BIOTECH VL - 48 PY - 2018 IS - 9 SP - 834 EP - 841 PG - 8 SN - 1082-6068 DO - 10.1080/10826068.2018.1514512 UR - https://m2.mtmt.hu/api/publication/30565437 ID - 30565437 AB - Bioethanol production from agro-industrial residues is gaining attention because of the limited production of starch grains and sugarcane, and food-fuel conflict. The aim of the present study is to maximize the bioethanol production using cassava bagasse as a feedstock. Enzymatic liquefaction, by alpha-amylase, followed by simultaneous saccharification and fermentation (SSF), using glucoamylase and Zymomonas mobilis MTCC 2427, was investigated for bioethanol production from cassava bagasse. The factors influencing ethanol production process were identified and screened for significant factors using Plackett-Burman design. The significant factors (cassava bagasse concentration (10-50 g/L), concentration of alpha-amylase (5-25% (v/v), and temperature of fermentation (27-37 degrees C)) were optimized by employing Box-Behnken design and genetic algorithm. The maximum ethanol concentrations of 25.594 g/L and 25.910 g/L were obtained from Box-Behnken design and genetic algorithm, respectively, under optimum conditions. Thus, the study provides valuable insights in utilizing the cost-effective industrial residue, cassava bagasse, for the bioethanol production. LA - English DB - MTMT ER - TY - JOUR AU - Xu, Youjie AU - Zhang, Meng AU - Roozeboom, Kraig AU - Wang, Donghai TI - Integrated bioethanol production to boost low-concentrated cellulosic ethanol without sacrificing ethanol yield JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 250 PY - 2018 SP - 299 EP - 305 PG - 7 SN - 0960-8524 DO - 10.1016/j.biortech.2017.11.056 UR - https://m2.mtmt.hu/api/publication/27570765 ID - 27570765 LA - English DB - MTMT ER - TY - JOUR AU - Ye, Guangying AU - Zeng, Defu AU - Zhang, Shuaishuai AU - Fan, Meishan AU - Zhang, Hongdan AU - Xie, Jun TI - Ethanol production from mixtures of sugarcane bagasse and Dioscorea composita extracted residue with high solid loading JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 257 PY - 2018 SP - 23 EP - 29 PG - 7 SN - 0960-8524 DO - 10.1016/j.biortech.2018.02.008 UR - https://m2.mtmt.hu/api/publication/27600332 ID - 27600332 LA - English DB - MTMT ER - TY - JOUR AU - Yu, Na AU - Tan, Li AU - Sun, Zhao-Yong AU - Tang, Yue-Qin AU - Kida, Kenji TI - Production of Bio-Ethanol by Integrating Microwave-Assisted Dilute Sulfuric Acid Pretreated Sugarcane Bagasse Slurry with Molasses JF - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY J2 - APPL BIOCHEM BIOTECH VL - 185 PY - 2018 IS - 1 SP - 191 EP - 206 PG - 16 SN - 0273-2289 DO - 10.1007/s12010-017-2651-9 UR - https://m2.mtmt.hu/api/publication/27570764 ID - 27570764 LA - English DB - MTMT ER - TY - JOUR AU - Ibrahim, N AU - Kamarudin, S K AU - Minggu, L J TI - Production of electricity from ethanol and ammonium based wastewater via photo-electrochemical process JF - INTERNATIONAL JOURNAL OF HYDROGEN ENERGY J2 - INT J HYDROGEN ENERG VL - 42 PY - 2017 IS - Kuala Lumpur SP - 9051 EP - 9062 PG - 12 SN - 0360-3199 DO - 10.1016/j.ijhydene.2016.04.137 UR - https://m2.mtmt.hu/api/publication/26752398 ID - 26752398 LA - English DB - MTMT ER - TY - JOUR AU - Kazeem, Muinat Olanike AU - Shah, Umi Kalsom Md AU - Baharuddin, Azhari Samsu AU - AbdulRahman, Nor' Aini TI - Prospecting Agro-waste Cocktail: Supplementation for Cellulase Production by a Newly Isolated Thermophilic B-licheniformis 2D55 JF - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY J2 - APPL BIOCHEM BIOTECH VL - 182 PY - 2017 IS - 4 SP - 1318 EP - 1340 PG - 23 SN - 0273-2289 DO - 10.1007/s12010-017-2401-z UR - https://m2.mtmt.hu/api/publication/26921451 ID - 26921451 LA - English DB - MTMT ER - TY - JOUR AU - Muller, Gerdt AU - Kalyani, Dayanand Chandrahas AU - Horn, Svein Jarle TI - LPMOs in cellulase mixtures affect fermentation strategies for lactic acid production from lignocellulosic biomass JF - BIOTECHNOLOGY AND BIOENGINEERING J2 - BIOTECHNOL BIOENG VL - 114 PY - 2017 IS - 3 SP - 552 EP - 559 PG - 8 SN - 0006-3592 DO - 10.1002/bit.26091 UR - https://m2.mtmt.hu/api/publication/26568195 ID - 26568195 N1 - Cited By :24 Export Date: 30 January 2024 CODEN: BIBIA LA - English DB - MTMT ER - TY - JOUR AU - Nair, Ramkumar B AU - Kalif, Mahdi AU - Ferreira, Jorge A AU - Taherzadeh, Mohammad J AU - Lennartsson, Patrik R TI - Mild-temperature dilute acid pretreatment for integration of first and second generation ethanol processes JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 245 PY - 2017 SP - 145 EP - 151 PG - 7 SN - 0960-8524 DO - 10.1016/j.biortech.2017.08.125 UR - https://m2.mtmt.hu/api/publication/27084550 ID - 27084550 LA - English DB - MTMT ER - TY - JOUR AU - Nongthombam, Grihalakshmi D AU - Labala, Rajendra K AU - Das, Sudripta AU - Handique, Pratap J AU - Talukdar, Narayan C TI - Evaluation and Selection of Potential Biomass Sources of North-East India towards Sustainable Bioethanol Production JF - FRONTIERS IN ENERGY RESEARCH J2 - FRONT ENERGY RES VL - 5 PY - 2017 PG - 13 SN - 2296-598X DO - 10.3389/fenrg.2017.00016 UR - https://m2.mtmt.hu/api/publication/27084551 ID - 27084551 LA - English DB - MTMT ER - TY - JOUR AU - Settle, Amy E AU - Berstis, Laura AU - Rorrer, Nicholas A AU - Roman-Leshkov, Yuriy AU - Beckham, Gregg T AU - Richards, Ryan M AU - Vardon, Derek R TI - Heterogeneous Diels-Alder catalysis for biomass-derived aromatic compounds JF - GREEN CHEMISTRY J2 - GREEN CHEM VL - 19 PY - 2017 IS - 15 SP - 3468 EP - 3492 PG - 25 SN - 1463-9262 DO - 10.1039/c7gc00992e UR - https://m2.mtmt.hu/api/publication/26921450 ID - 26921450 LA - English DB - MTMT ER - TY - JOUR AU - Xu, Youjie AU - Wang, Donghai TI - Integrating starchy substrate into cellulosic ethanol production to boost ethanol titers and yields JF - APPLIED ENERGY J2 - APPL ENERG VL - 195 PY - 2017 SP - 196 EP - 203 PG - 8 SN - 0306-2619 DO - 10.1016/j.apenergy.2017.03.035 UR - https://m2.mtmt.hu/api/publication/26752397 ID - 26752397 LA - English DB - MTMT ER - TY - JOUR AU - Akbas, Meltem Yesilcimen AU - Stark, Benjamin C TI - Recent trends in bioethanol production from food processing byproducts JF - JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY J2 - J IND MICROBIOL BIOT VL - 43 PY - 2016 IS - 11 SP - 1593 EP - 1609 PG - 17 SN - 1367-5435 DO - 10.1007/s10295-016-1821-z UR - https://m2.mtmt.hu/api/publication/26228765 ID - 26228765 LA - English DB - MTMT ER - TY - JOUR AU - Dai, Jing AU - Bean, Brent AU - Brown, Bradford AU - Bruening, William AU - Edwards, Jeff AU - Flowers, Michael AU - Karow, Russell AU - Lee, Chad AU - Morgan, Gaylon AU - Ottman, Michael AU - Ransom, Joel AU - Wiersma, Jochum TI - Harvest index and straw yield of five classes of wheat JF - BIOMASS & BIOENERGY J2 - BIOMASS BIOENERGY VL - 85 PY - 2016 SP - 223 EP - 227 PG - 5 SN - 0961-9534 DO - 10.1016/j.biombioe.2015.12.023 UR - https://m2.mtmt.hu/api/publication/25428772 ID - 25428772 LA - English DB - MTMT ER - TY - JOUR AU - Ji, Li AU - Zheng, Tianran AU - Zhao, Pengxiang AU - Zhang, Weiming AU - Jiang, Jianxin TI - Ethanol production from a biomass mixture of furfural residues with green liquor-peroxide saccarified cassava liquid JF - BMC BIOTECHNOLOGY J2 - BMC BIOTECHNOL VL - 16 PY - 2016 PG - 11 SN - 1472-6750 DO - 10.1186/s12896-016-0278-5 UR - https://m2.mtmt.hu/api/publication/26041116 ID - 26041116 LA - English DB - MTMT ER - TY - JOUR AU - Ji, Shi-Qi AU - Wang, Bing AU - Lu, Ming AU - Li, Fu-Li TI - Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 9 PY - 2016 PG - 10 SN - 1754-6834 DO - 10.1186/s13068-016-0494-1 UR - https://m2.mtmt.hu/api/publication/25799133 ID - 25799133 LA - English DB - MTMT ER - TY - JOUR AU - Joelsson, Elisabeth AU - Erdei, Borbala AU - Galbe, Mats AU - Wallberg, Ola TI - Techno-economic evaluation of integrated first- and second-generation ethanol production from grain and straw JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 9 PY - 2016 PG - 16 SN - 1754-6834 DO - 10.1186/s13068-015-0423-8 UR - https://m2.mtmt.hu/api/publication/25360257 ID - 25360257 LA - English DB - MTMT ER - TY - JOUR AU - Ma, Wei AU - Duan, Shibo AU - Tan, Dazhi AU - Cheng, Zihong AU - Meng, Fanqing AU - Yang, Lei AU - Li, Xuechao TI - Reuse of concentrated cellulose wastewater: Microwave-assisted synthesis of organic fertilizer with water-retaining property JF - 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