@article{MTMT:34609146, title = {The 2022 Russia invasion on Ukraine: The biofuel energy security challenge on Ukraine and some related countries}, url = {https://m2.mtmt.hu/api/publication/34609146}, author = {Esonye, Chizoo and Esonye, Constance Okechukwu and Agha, Emmanuel Obiahu and Ume, Cyril Sunday and Njemanze, Chizoma Vivian and Eyisi, Chimezie Emmanuel and Adepoju, Tunde Folunsho}, doi = {10.1016/j.heliyon.2023.e21483}, journal-iso = {HELIYON}, journal = {HELIYON}, volume = {9}, unique-id = {34609146}, abstract = {Europe, United States and other countries make moves to compensate for the loss of Ukraine's exports by diverting crops meant for biofuels into food production and to relax some of the biofuels mandates due to the challenges of 2022 Russian invasion on Ukraine (RIU). Ukraine as a leading exporter of cereals and oil crops in the world has suffered reduction in exports of grain products since the inception of the invasion. This work critically evaluates the immediate and remote potential effects of the RIU on Ukraine's biofuel capacity and potentials, energy strategies and policies, direct impacts of the war on Ukraine and Russia and other countries' biofuels policies were evaluated. Ukraine is found to be a very important big player on global biofuels energy security and policies. It was observed that there are more significant impacts of the war on Ukraine biofuel industry than that of Russia due to very low priority on the development of Liquid biofuels (LBs) in Russia. Recommendations based on the concepts of national energy security and independence were elucidated. Post invasion-strategies such as development of framework for management of post-war waste, ensuring strict adherence to EU biofuels sustainable directives, and implementation of Bioplus-project for Ukraine government were underpinned. This article provides useful information, guidelines and directives that would enable Ukraine government and other countries facing energy insecurity and political crises to restore energy independence and national economy.}, keywords = {sustainability; policy; STRATEGY; Biofuels; Energy security; Russia -Ukraine invasion}, year = {2023}, eissn = {2405-8440} } @article{MTMT:34278442, title = {A framework for the design of sustainable multi-input second-generation biorefineries through process simulation: A case study for the valorization of lignocellulosic and starchy waste from the plantain agro-industry}, url = {https://m2.mtmt.hu/api/publication/34278442}, author = {Gomez, James A. and Matallana, Luis G. and Teixeira, Jose A. and Sanchez, Oscar J.}, doi = {10.1016/j.cherd.2023.06.004}, journal-iso = {CHEM ENG RES DES}, journal = {CHEMICAL ENGINEERING RESEARCH AND DESIGN}, volume = {195}, unique-id = {34278442}, issn = {0263-8762}, abstract = {The plantain agro-industry generates different residues in the harvest and post-harvest stages. Therefore, new processes for its valorization are required. The aim of this research was to propose a general methodological framework for the design and analysis of multi -input biorefineries for the valorization of residues with a high content of lignocellulosic and starchy materials. This approach was based on the independent processing of the starchy and lignocellulosic materials in the first steps of the biorefinery and relies on process simulation and hierarchic process design procedures. A case study for the design of a multi-input biorefinery for the valorization of residues from the plantain agro-in-dustry was performed by applying the framework proposed. The biorefinery was simu-lated using SuperPro Designer software. The results obtained suggest that the best alternative for the valorization of plantain residues under Colombian conditions corre-sponded to the following distribution of key residues: 100% leaf sheaths processed into the natural fiber production section, and 25% peels and 25% rachis processed into the starch extraction and liquefaction section; with this distribution, an NPV of $21,348,000 can be achieved. This work shows that waste from the plantain agro-industry exhibits high po-tential as a feedstock for the production of value-added products.& COPY; 2023 The Authors. Published by Elsevier Ltd on behalf of Institution of Chemical Engineers. This is an open access article under the CC BY-NC-ND license (http://creati-vecommons.org/licenses/by-nc-nd/4.0/).}, keywords = {anaerobic digestion; Starch hydrolysis; CELLULOSE HYDROLYSIS; Process scheduling; Process flowsheeting; Musa AAB Simmonds}, year = {2023}, eissn = {1744-3563}, pages = {551-571} } @article{MTMT:33891816, title = {Comparative study of ethanol production from sodium hydroxide pretreated rice straw residue using Saccharomyces cerevisiae and Zymomonas mobilis}, url = {https://m2.mtmt.hu/api/publication/33891816}, author = {Kumar, Naveen and Yadav, Anita and Singh, Gulab and Singh, Ajay and Kumar, Pankaj and Aggarwal, Neeraj K.}, doi = {10.1007/s00203-023-03468-1}, journal-iso = {ARCH MICROBIOL}, journal = {ARCHIVES OF MICROBIOLOGY}, volume = {205}, unique-id = {33891816}, issn = {0302-8933}, abstract = {Rice straw is a suitable alternative to a cheaper carbohydrate source for the production of ethanol. For pretreatment efficiency, different sodium hydroxide concentrations (0.5-2.5% w/v) were tested. When compared to other concentrations, rice straw processed with 2% NaOH (w/v) yielded more sugar (8.17 +/- 0.01 mg/ml). An alkali treatment induces effective delignification and swelling of biomass. The pretreatment of rice straw with 2% sodium hydroxide (w/v) is able to achieve 55.34% delignification with 53.30% cellulose enrichment. The current study shows the effectiveness of crude cellulolytic preparation from Aspergillus niger resulting in 80.51 +/- 0.4% cellulose hydrolysis. Rice straw hydrolysate was fermented using ethanologenic Saccharomyces cerevisiae (yeast) and Zymomonas mobilis (bacteria). Overall, superior efficiency of sugar conversion to ethanol 70.34 +/- 0.3% was obtained with the yeast compared to bacterial strain 39.18 +/- 0.5%. The current study showed that pretreatment with sodium hydroxide is an effective method for producing ethanol from rice straw and yeast strain S. cerevisiae having greater fermentative potential for bioethanol production than bacterial strain Z. mobilis.}, keywords = {Ethanol; FERMENTATION; PRETREATMENT; enzymatic hydrolysis; Rice straw}, year = {2023}, eissn = {1432-072X} } @article{MTMT:33891818, title = {Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch}, url = {https://m2.mtmt.hu/api/publication/33891818}, author = {Li, Hong-Fei and Tian, Linyue and Lian, Guoli and Fan, Li-Hai and Li, Zheng-Jun}, doi = {10.3389/fbioe.2023.1130368}, journal-iso = {FRONT BIOENG BIOTECHNOL}, journal = {FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY}, volume = {11}, unique-id = {33891818}, issn = {2296-4185}, abstract = {Vibrio alginolyticus LHF01 was engineered to efficiently produce poly-3-hydroxybutyrate (PHB) from starch in this study. Firstly, the ability of Vibrio alginolyticus LHF01 to directly accumulate PHB using soluble starch as the carbon source was explored, and the highest PHB titer of 2.06 g/L was obtained in 18 h shake flask cultivation. Then, with the analysis of genomic information of V. alginolyticus LHF01, the PHB synthesis operon and amylase genes were identified. Subsequently, the effects of overexpressing PHB synthesis operon and amylase on PHB production were studied. Especially, with the co-expression of PHB synthesis operon and amylase, the starch consumption rate was improved and the PHB titer was more than doubled. The addition of 20 g/L insoluble corn starch could be exhausted in 6-7 h cultivation, and the PHB titer was 4.32 g/L. To the best of our knowledge, V. alginolyticus was firstly engineered to produce PHB with the direct utilization of starch, and this stain can be considered as a novel host to produce PHB using starch as the raw material.}, keywords = {Starch; amylase; metabolic engineering; Vibrio alginolyticus; Poly-3-hydroxybutyrate}, year = {2023}, eissn = {2296-4185}, orcid-numbers = {Tian, Linyue/0000-0003-1503-0950} } @article{MTMT:33891817, title = {High-pressure microwave-assisted pretreatment of softwood, hardwood and non-wood biomass using different solvents in the production of cellulosic ethanol}, url = {https://m2.mtmt.hu/api/publication/33891817}, author = {Mikulski, Dawid and Klosowski, Grzegorz}, doi = {10.1186/s13068-023-02272-9}, journal-iso = {BIOTECHN BIOFUEL BIOPROD}, journal = {BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS}, volume = {16}, unique-id = {33891817}, abstract = {BackgroundPretreatment is an indispensable stage of the preparation of lignocellulosic biomass with key significance for the effectiveness of hydrolysis and the efficiency of the production of cellulosic ethanol. A significant increase in the susceptibility of the raw material to further degradation can be attained as a result of effective delignification in high-pressure conditions. With this in mind, a method of high-pressure pretreatment using microwave radiation and various solvents (water, 40% w/v NaCS, 1% v/v H2SO4, 1% w/v NaOH or 60% v/v EtOH with an addition of 1% v/v H2SO4) was developed, enabling the acquisition of biomass with an increased susceptibility to the process of enzymatic hydrolysis. The medium obtained in this way can be used for the production of cellulosic ethanol via high-gravity technology (lignocellulosic media containing from 15 to 20% dry weight of biomass). For every type of biomass (pine chips, beech chips and wheat straw), a solvent was selected to be used during the pretreatment, guaranteeing the acquisition of a medium highly susceptible to the process of enzymatic hydrolysis.ResultsThe highest efficiency of the hydrolysis of biomass, amounting to 71.14 +/- 0.97% (glucose concentration 109.26 +/- 3.49 g/L) was achieved for wheat straw subjected to microwave-assisted pretreatment using 40% w/v NaCS. Fermentation of this medium produced ethanol concentration at the level of 53.84 +/- 1.25 g/L. A slightly lower effectiveness of enzymatic hydrolysis (62.21 +/- 0.62%) was achieved after high-pressure microwave-assisted pretreatment of beech chips using 1% w/v NaOH. The hydrolysate contained glucose in the concentration of 91.78 +/- 1.91 g/L, and the acquired concentration of ethanol after fermentation amounted to 49.07 +/- 2.06 g/L. In the case of pine chips, the most effective delignification was achieved using 60% v/v EtOH with the addition of 1% v/v H2SO4, but after enzymatic hydrolysis, the concentration of glucose in hydrolysate was lower than in the other raw materials and amounted to 39.15 +/- 1.62 g/L (the concentration of ethanol after fermentation was ca. 19.67 +/- 0.98 g/L). The presence of xylose and galactose was also determined in the obtained fermentation media. The highest initial concentration of these carbohydrates (21.39 +/- 1.44 g/L) was observed in beech chips media after microwave-assisted pretreatment using NaOH. The use of wheat straw after pretreatment using EtOH with an addition of 1% v/v H2SO4 for the preparation of fermentation medium, results in the generation of the initial concentration of galactose and xylose at the level of 19.03 +/- 0.38 g/L.ConclusionThe achieved results indicate a high effectiveness of the enzymatic hydrolysis of the biomass subjected to high-pressure microwave-assisted pretreatment. The final effect depends on the combined use of correctly selected solvents for the different sources of lignocellulosic biomass. On the basis of the achieved results, we can say that the presented method indicates a very high potential in the area of its use for the production of cellulosic ethanol involving high-gravity technology.}, keywords = {BIOMASS; Bioethanol; High-pressure microwave-assisted pretreatment; HG technology}, year = {2023}, eissn = {2731-3654}, orcid-numbers = {Mikulski, Dawid/0000-0001-9989-3029} } @article{MTMT:33891815, title = {Delignification and Densification as a Route to Enable the Use of Wheat Straw for Structural Materials}, url = {https://m2.mtmt.hu/api/publication/33891815}, author = {Neudecker, Felix and Jakob, Matthias and Bodner, Sabine C. and Keckes, Jozef and Buerstmayr, Hermann and Gindl-Altmutter, Wolfgang}, doi = {10.1021/acssuschemeng.3c01375}, journal-iso = {ACS SUSTAIN CHEM ENG}, journal = {ACS SUSTAINABLE CHEMISTRY & ENGINEERING}, unique-id = {33891815}, issn = {2168-0485}, abstract = {Cereal straw is an abundant byproduct of crop production. Despite little current use in materials, the biologically optimized structure, which carries the weight of a structure up to 1.5 m high and protects it against environmental impacts, shows considerable potential. In order to be able to use this renewable material for structural purposes, barriers such as small dimensions and a low density have to be overcome. In the present study, lignin was partially removed from wheat straw using an alkaline solution with the aim of increasing the relative cellulose content and improving malleability. Thereafter, a homogeneous, dense, and multi layered material was produced by densification at elevated temperatures. Outstanding improvements in mechanical performance were obtained compared to the untreated raw material. For the optimum variant, tensile strength increased by a factor of three (average strength 218 MPa) and elastic modulus increased by a factor of four (average 13 GPa), which is within the range of established wood-based structural materials. We propose that mild alkaline treatment as carried out in the present study may significantly facilitate the development of high-value structural materials based on little utilized cereal straw.}, keywords = {DELIGNIFICATION; tensile strength; Densification; AGRICULTURAL BY-PRODUCTS; wheat straw; Alkaline treatment}, year = {2023}, eissn = {2168-0485} } @article{MTMT:33891819, title = {Recent advances in consolidated bioprocessing for conversion of lignocellulosic biomass into bioethanol-A review}, url = {https://m2.mtmt.hu/api/publication/33891819}, author = {Periyasamy, Selvakumar and Isabel, J. Beula and Kavitha, S. and Karthik, V. and Mohamed, Badr A. and Gizaw, Desta Getachew and Sivashanmugam, P. and Aminabhavi, Tejraj M.}, doi = {10.1016/j.cej.2022.139783}, journal-iso = {CHEM ENG J}, journal = {CHEMICAL ENGINEERING JOURNAL}, volume = {453}, unique-id = {33891819}, issn = {1385-8947}, abstract = {As the world's population grows, it will be necessary to make long-term technical advancements to meet energy needs without jeopardizing the planet's scarce resources. There are several benefits to using renewable energy systems, such as stabilizing energy supply and demand, ensuring food security and economic stability, and protecting the environment from pollution. Bioethanol is a possible alternative for a renewable and long-term energy source. However, for long-term survival and economic viability, it is critical to focus on lower-cost ethanol production for this fuel to compete with petroleum. To make biofuel commercially viable, converting lignocellulose to bioethanol might be more cost-effective, environmentally friendly, and efficient, but it would still require substantial process research and optimization. The production cost of value-added products, i.e., bioethanol, can be reduced by using consolidated bioprocessing to combine enzyme synthesis and secretion with the hydrolysis of polysaccharides and fermentation of accessible sugars in a single operation. This review focuses on biomass pretreatments, process enhancements, recombinant microbial catalysts and enzymes, and metabolic engineering to consolidate bioprocessing. Besides, it delivers an overview to facilitate future research in ligno-cellulosic biomass for bioethanol production.}, keywords = {PRETREATMENT; Bioethanol; lignocellulose; microbial consortium; consolidated bioprocessing}, year = {2023}, eissn = {1873-3212}, orcid-numbers = {Mohamed, Badr A./0000-0002-5550-886X; Aminabhavi, Tejraj M./0000-0002-5613-3916} } @article{MTMT:34278443, title = {Biorefineries development from agricultural byproducts: Value addition and circular bioeconomy}, url = {https://m2.mtmt.hu/api/publication/34278443}, author = {Srivastava, Rajesh K. and Sarangi, Prakash Kumar and Shadangi, Krushna Prasad and Sasmal, Soumya and Gupta, Vijai Kumar and Govarthanan, Muthusamy and Sahoo, Uttam Kumar and Subudhi, Sanjukta}, doi = {10.1016/j.scp.2023.100970}, journal-iso = {SUSTAIN CHEM PHARM}, journal = {SUSTAINABLE CHEMISTRY AND PHARMACY}, volume = {32}, unique-id = {34278443}, issn = {2352-5541}, abstract = {The increase in the human population in the world, industrialization, and rapid urbanization cre-ates a big challenge for the agricultural revolution. Hence, to enhance the crop's productivity modern agricultural technology, hybrid seeds; management of full irrigation facilities, and the use of various fertilizers, and pesticides are being adopted. Though such advanced techniques en-hance productivity, they also produce a huge amount of agro-waste. The proper waste manage-ment of such wastes is a headache for the farmers due to the lack of dumping lands, proper trans-portation, removal facilities, and lack of planned utilization. Since direct burning of the wastes do air, water, and soil pollution, a systematic utilization of these cheap wastes for the development of value-added products and chemicals will promote a clean and healthy environment for the bi-otic and abiotic components. A proper, efficient, and effective treatment system can break down waste biomass into simple forms and can be easily consumed by our natural and engineered mi-crobial systems to produce bio-chemicals and bio-fuels. Value-added products from agro wastes are now needed every hour as far as the sustainable production of energy and biochemical is an important concern. Agro-waste utilization-based biorefineries can provide a big pillar for the de-velopment of a circular bio-economy. The review emphasizes the value-added products gener-ated from agricultural byproducts/waste residue's utility, towards sustainability and a clean envi-ronment.}, keywords = {sustainability; Pre-Treatment; agrowaste; Components analysis; Chemicals synthesis}, year = {2023}, eissn = {2352-5541}, orcid-numbers = {Sasmal, Soumya/0000-0002-9763-2500} } @article{MTMT:33003549, title = {Enzymatic and Acidic Hydrolysis of Cull Potatoes for Production of Fermentable Sugars}, url = {https://m2.mtmt.hu/api/publication/33003549}, author = {Bansal, Riya and Katyal, Priya and Jain, Deepali}, doi = {10.1002/star.202100202}, journal-iso = {STARCH-STARKE}, journal = {STARCH-STARKE}, volume = {74}, unique-id = {33003549}, issn = {0038-9056}, abstract = {Cull potato is an under-utilized biomass being produced in different states of India. Using cull potatoes as a substrate for bioethanol production can overcome the post-harvest losses as well as making the process economical. In the present study, comparative hydrolysis of cull potatoes is carried out using enzymes and hydrochloric acid to study the effect of variables on saccharification efficiency. For enzymatic hydrolysis, samples are liquefied using crude enzyme from Bacillus sp. and then saccharification of liquefied samples is optimized using central composite rotatable design (CCRD) in Response surface methodology (RSM) w.r.t pH (4-7), temperature (25-40 degrees C) and incubation period (15-60 min) at 5% substrate concentration with glucoamylase of Aspergillus niger. Acidic hydrolysis is performed at different acid concentration, i.e., 1-5 (v/v) at variable time intervals (15-60 min) with 1-5% substrate concentration at 90 degrees C. Highest saccharification efficiency is observed with acidic hydrolysis 85.2% at substrate concentration of (4%) and at acid concentration (4% v/v) after 45 min of incubation, while with enzymatic hydrolysis it is very close to acidic hydrolysis, i.e., 83.2% at 25 degrees C temperature and at pH 4 after 30 min of incubation.}, keywords = {Glucoamylase; Response surface methodology (RSM); Aspergillus spp; cull potatoes; saccharification efficiency}, year = {2022}, eissn = {1521-379X}, orcid-numbers = {Bansal, Riya/0000-0002-5314-5452} } @article{MTMT:33873645, title = {A plasmid-free Zymomonas mobilis mutant strain reducing reactive oxygen species for efficient bioethanol production using industrial effluent of xylose mother liquor}, url = {https://m2.mtmt.hu/api/publication/33873645}, author = {Geng, Binan and Liu, Shuyi and Chen, Yunhao and Wu, Yalun and Wang, Yi and Zhou, Xuan and Li, Han and Li, Mian and Yang, Shihui}, doi = {10.3389/fbioe.2022.1110513}, journal-iso = {FRONT BIOENG BIOTECHNOL}, journal = {FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY}, volume = {10}, unique-id = {33873645}, issn = {2296-4185}, abstract = {Genome minimization is an effective way for industrial chassis development. In this study, Zymomonas mobilis ZMNP, a plasmid-free mutant strain of Z. mobilis ZM4 with four native plasmids deleted, was constructed using native type I-F CRISPR-Cas system. Cell growth of ZMNP under different temperatures and industrial effluent of xylose mother liquor were examined to investigate the impact of native plasmid removal. Despite ZMNP grew similarly as ZM4 under different temperatures, ZMNP had better xylose mother liquor utilization than ZM4. In addition, genomic, transcriptomic, and proteomic analyses were applied to unravel the molecular changes between ZM4 and ZMNP. Whole-genome resequencing result indicated that an S267P mutation in the C-terminal of OxyR, a peroxide-sensing transcriptional regulator, probably alters the transcription initiation of antioxidant genes for stress responses. Transcriptomic and proteomic studies illustrated that the reason that ZMNP utilized the toxic xylose mother liquor better than ZM4 was probably due to the upregulation of genes in ZMNP involving in stress responses as well as cysteine biosynthesis to accelerate the intracellular ROS detoxification and nucleic acid damage repair. This was further confirmed by lower ROS levels in ZMNP compared to ZM4 in different media supplemented with furfural or ethanol. The upregulation of stress response genes due to the OxyR mutation to accelerate ROS detoxification and DNA/RNA repair not only illustrates the underlying mechanism of the robustness of ZMNP in the toxic xylose mother liquor, but also provides an idea for the rational design of synthetic inhibitor-tolerant microorganisms for economic lignocellulosic biochemical production.}, keywords = {proteomics; Reactive oxygen species (ROS); OXYR; Zymomonas mobilis; Whole-genome resequencing (WGR); transcriptomics (RNA-seq); plasmid-free; xylose mother liquor}, year = {2022}, eissn = {2296-4185} } @article{MTMT:34278444, title = {THE SIGNIF ICANCE OF HARVEST RESIDUES IN THE SUSTAINABLE MANAGEMENT OF ARABLE LAND. II. HARVEST RESIDUES MANAGEMENT}, url = {https://m2.mtmt.hu/api/publication/34278444}, author = {Novo, Przulj and Vesna, Tunguz and Zoran, Jovovic and Ana, Velimirovic}, doi = {10.7251/afts.2022.1427.049PCOBISS.RS-ID137253377}, journal-iso = {ARCH TECHN SCI}, journal = {ARHIV ZA TEHNICKE NAUKE / ARCHIVES FOR TECHNICAL SCIENCES}, unique-id = {34278444}, issn = {1840-4855}, abstract = {Harvest residues (HR) are one of the important aspects of sustainable management in agriculture, representing a significant portion of organic matter (OM) that can be retained or removed from agro-ecosystems. There are several ways to manage plant residues: (i) burning, (ii) incorporation in soil, (iii) leaving plant residues after harvest on the soil surface in form of mulch, (iv) undersowing crops in HR and (v) baling and removing HR from the plot. Burning is the most unreasonable action in agricultural production, which is prohibited by legal regulations in force. The burning means a complete loss of OM -N and S are irretrievably lost in the air, mineral substances remain in ash, and microorganisms in the soil surface layer are destroyed. Incorporation of HR may be complete or partial, depending on the soil cultivation method applied.This HR management method has a number of soil benefits: increases content of nutrients, OM and humus; increases microbiological activity; improves soil water, air and physical properties; improves soil structure, ultimately contributing to the increase in soil fertility and plant growth. Two practical approaches that were once common, have been neglected. Namely, undersowing and mixed cultivation where various species are simultaneously grown with or between plantings of a main crop. Removal of HR indirectly leads to a decrease of the organic matter in the soil. In essence, there is a need to determine the size of acceptors and the return rates of organic residues of different quality, and to increase the efficiency of nutrient cycling from residues through various land acceptors and crops, with minimal losses from the system.}, keywords = {soil quality; Soil organic matter; Harvest residues; incorporation of harvest residues}, year = {2022}, eissn = {2233-0046}, pages = {49-56} } @article{MTMT:33224765, title = {Integrated biorefinery approaches for the industrialization of cellulosic ethanol fuel}, url = {https://m2.mtmt.hu/api/publication/33224765}, author = {Qiao, Jie and Cui, Haiyang and Wang, Minghui and Fu, Xianshen and Wang, Xinyue and Li, Xiujuan and Huang, He}, doi = {10.1016/j.biortech.2022.127516}, journal-iso = {BIORESOUR TECHNOL}, journal = {BIORESOURCE TECHNOLOGY}, volume = {360}, unique-id = {33224765}, issn = {0960-8524}, abstract = {Lignocellulosic biomass is an abundant and sustainable raw material, but its conversion into ethanol fuel has not yet achieved large-scale industrialization and economic benefits. Integrated biorefineries have been widely identified as the key to achieving this goal. Here, four promising routes were summarized to assemble the new industrial plants for cellulose-based fuels and chemicals, including 1) integration of cellulase production systems into current cellulosic ethanol processes; 2) combination of processes and facilities between cellulosic ethanol and first-generation ethanol; 3) application of enzyme-free saccharification processes and computational ap-proaches to increase the bioethanol yield and optimize the integration process; 4) production of multiple products to maximize the value derived from the lignocellulosic biomass. Finally, the remaining challenges and perspectives of this field are also discussed.}, keywords = {Ethanol; LIGNOCELLULOSIC BIOMASS; integrated biorefinery}, year = {2022}, eissn = {1873-2976}, orcid-numbers = {Cui, Haiyang/0000-0001-8360-0447; Li, Xiujuan/0000-0001-7642-721X} } @article{MTMT:33645608, title = {Effect of Fertilization with Meat and Bone Meal on the Production of Biofuel Obtained from Corn Grain}, url = {https://m2.mtmt.hu/api/publication/33645608}, author = {Stępień, Arkadiusz and Rejmer, Wojciech}, doi = {10.3390/en16010021}, journal-iso = {ENERGIES}, journal = {ENERGIES}, volume = {16}, unique-id = {33645608}, issn = {1996-1073}, abstract = {The large volumes of meat and bone meal (MBM) being produced are leading to an increased demand for research into innovative methods of utilizing MBM and obtaining further benefits. The object of this study is to analyze the efficiency of bioethanol and biodiesel production obtained from corn grain fertilized with meat and bone meal produced from animal waste. For the realization of this study, a four-year field experiment was carried out with grain corn fertilized with different doses of meat and bone meal in comparison to fertilization with mineral fertilizers and no fertilization. Fertilization with meat and bone meal should be considered not only for its direct effect but also for the after-effect. The effect of meat and bone meals on obtaining a grain yield higher than that obtained on objects without fertilization and those fertilized with mineral fertilizers was noticeable after applying higher doses from the third year of the study. Fertilization with meat and bone meals did not significantly affect the average fat content of grain, and it only slightly affected the starch content. The positive effect of meat and bone meals on the yield of bioethanol from grain extracted from one hectare was responsible for their yield-forming effect. The differences obtained between years and between fertilizer variants in the yields of ethyl biodiesel and methyl biodiesel per one hectare were mainly related to grain yields, rather than the obtained volume per 1 kg from grain.}, year = {2022}, eissn = {1996-1073}, pages = {21}, orcid-numbers = {Stępień, Arkadiusz/0000-0002-1138-9715; Rejmer, Wojciech/0000-0002-1955-1553} } @article{MTMT:32391955, title = {Emerging Synergies on the Co-treatment of Spent Coffee Grounds and Brewer's Spent Grains for Ethanol Production}, url = {https://m2.mtmt.hu/api/publication/32391955}, author = {Barampouti, Elli Maria and Grammatikos, Christos and Stoumpou, Vasileia and Malamis, Dimitris and Mai, Sofia}, doi = {10.1007/s12649-021-01543-6}, journal-iso = {WASTE BIOMASS VALORI}, journal = {WASTE AND BIOMASS VALORIZATION}, unique-id = {32391955}, issn = {1877-2641}, abstract = {Purpose Given that the depletion of fossil fuels is an important issue that should be tackled urgently, alternative routes are being sought for biofuels production. This paper, under this context, studied the potential synergies of co-treatment of two kinds of agro-waste (brewers' spent grains, BSG and spent coffee grounds, SCG) towards the direction of biodiesel and bioethanol production, alleviating at the same time their treatment and management issues. Methods The technical feasibility of oil extraction and ethanol production was studied and optimized in both laboratory and bench scale. A treatment train including alkaline pretreatment of SCG and dilute acid pretreatment of BSG, neutralization of the solutions by mixing them, enzymatic saccharification and ethanolic fermentation led to the production of bioethanol. Results The co-treatment of BSG and SCG proved beneficial and optimum experimental conditions were defined by setting the bioethanol production cost as optimization parameter. The lowest ethanol production cost (3.9euro/kg) was observed for scenario 4 (SCG/BSG mixture, chemical pretreatment (0.5 N, 5 h), enzymatic hydrolysis (400 mu L/g cellulose, 16 h)) and scenario 5 (SCG/BSG mixture, chemical pretreatment (0.5 N, 5 h), enzymatic hydrolysis (800 mu L/g cellulose, 5 h)). Further upscaling of the process verified the technical feasibility of process units leading to a further increase of ethanol yield and thus a decrease in the production cost. Conclusions Conclusively, it was demonstrated that the recovery and extraction of the oil content of BSG and SCG and the achievement of high ethanol yields is technically feasible. Within this context, an integrated biorefinery was presented, making evident that these substrates could reverse the eminent energy crisis via biofuels production. Graphic Abstract}, keywords = {enzymatic hydrolysis; Agro-waste; Valorisation; oil extraction}, year = {2021}, eissn = {1877-265X}, orcid-numbers = {Barampouti, Elli Maria/0000-0003-4871-8786} } @article{MTMT:32391956, title = {Pretreatment of finger millet straw (Eleusine coracana) for enzymatic hydrolysis towards bioethanol production}, url = {https://m2.mtmt.hu/api/publication/32391956}, author = {Lamichhane, Ganesh and Khadka, Sujan and Acharya, Ashis and Parajuli, Niranjan}, doi = {10.1007/s13399-021-01633-4}, journal-iso = {BIOMASS CONV BIOREFINERY}, journal = {BIOMASS CONVERSION AND BIOREFINERY}, unique-id = {32391956}, issn = {2190-6815}, abstract = {To obtain high yields of products through bioconversion processes, biomass must be pretreated to change its recalcitrant structures. The key goal of this analysis is to compare the findings in terms of total reducing sugar yield after organic solvents and hot water pretreatment on finger millet straw (FMS) using laboratory-scale equipment. Accordingly, the effect of two separate pretreatments, i.e., liquid hot water, and organic solvent (ethanol) at autoclave, and furnace temperatures, was investigated in this study to increase the enzymatic conversion of FMS under optimal conditions. The biomass recovery was found highest under the liquid hot water (autoclave; 9.477 +/- 0.017%) pretreatment conditions. In addition, the cellulose content improved significantly with pretreatment, increasing from 41.7 +/- 0.74 to 55.33 +/- 0.89%, while the hemicellulose and lignin contents significantly decreased. The untreated sample having a lignin amount of around 6.42 +/- 0.21% after different pretreatment methods dropped significantly (p = 0.00737**) and was found to have a 39.8 +/- 1.84% delignification rate based on the ethanol autoclave method. The findings of the study revealed that the FMS is severely affected by liquid hot water and organic solvent pretreatment in the autoclave than the furnace. After the pretreatment procedure, the maximum amount of crystalline cellulose in biomass was transformed to the amorphous state, as shown by the Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The pretreated cellulase blend enzyme sample was most active on the biomass treated with 1:20% (w/v) liquid hot water combined with autoclave heating at 121 degrees C, with 15 psi for 20 min, resulting in highest total reducing sugars; thus, results showed that the pretreated sample (0.75 g) contains 60.94 mg/g of fermentative sugars based on a 15% (w/v) ratio which claimed to be the fact that this is a better result than any other research by using FMS.}, keywords = {organic solvent; FINGER MILLET; total reducing sugars; Cellulase blend enzyme}, year = {2021}, eissn = {2190-6823}, orcid-numbers = {Lamichhane, Ganesh/0000-0002-4352-7457; Khadka, Sujan/0000-0003-1451-7804; Acharya, Ashis/0000-0002-9369-7219; Parajuli, Niranjan/0000-0002-9233-6489} } @article{MTMT:32391957, title = {Recent advances in bioethanol production from Lignocellulosic biomass}, url = {https://m2.mtmt.hu/api/publication/32391957}, author = {Lamichhane, Ganesh and Acharya, Ashis and Poudel, Darbin Kumar and Aryal, Babita and Gyawali, Narayan and Niraula, Purushottam and Phuyal, Sita Ram and Budhathoki, Prakriti and Bk, Ganesh and Parajuli, Niranjan}, doi = {10.1080/15435075.2021.1880910}, journal-iso = {INT J GREEN ENERGY}, journal = {INTERNATIONAL JOURNAL OF GREEN ENERGY}, volume = {18}, unique-id = {32391957}, issn = {1543-5075}, abstract = {The field of bioethanol production has seen rapid advancements in the past few decades. Despite the advancements, bioethanol production's recent trends cannot prove effective and efficient transportation fuel. Fossil fuels, which currently power-ups most energy demands, from nonrenewable energies, meaning it will bring adverse effects to the environment, including ozone layer depletion and global warming. It makes bioethanol the best alternative to fossil fuels, owing to the fact than it is considered carbon-neutral. In some parts of the world, bioethanol is utilized as an alternative fuel by using the lignocellulosic (LC) residues, and prioritizing its production as better feedstocks available could fulfill the void created in the transportation fuel sector. Separate hydrolysis and co-fermentation (SSCF) and Pre-hydrolysis and simultaneous saccharification and co-fermentation (PSSCF) are superior to all techniques to achieve saccharification, hexose, and pentose fermentation in a one-pot reaction. For the feedstock modification, the genome engineering and cell wall modification are advanced methods so far. As of not long ago, there is no cost-effective and financially achievable pretreatment technique for the removal of lignin that could be industrialized to satisfy the worldwide requirement for bioethanol. In this review we provide the present scenario of energy demand and advancement on bioethanol technology and challenges. Mainly, altering the LC biomass composition, the robust enzyme that enables to hydrolyzed and maximum fermentable sugar yield, and effectively fermenting bacteria and yeast, which is genetically engineered for simultaneous fermentation with the high rate, is future stepping research which should be emphasized in the bioethanol industry.}, keywords = {Bioethanol; Alternative fuel; Thermotolerant yeast; Robust enzyme; Future model}, year = {2021}, eissn = {1543-5083}, pages = {731-744}, orcid-numbers = {Lamichhane, Ganesh/0000-0002-4352-7457; Acharya, Ashis/0000-0002-9369-7219; Gyawali, Narayan/0000-0001-7810-7260; Niraula, Purushottam/0000-0003-0562-6275; Budhathoki, Prakriti/0000-0001-9768-459X; Parajuli, Niranjan/0000-0002-9233-6489} } @article{MTMT:33003548, title = {Degree of Biomass Conversion in the Integrated Production of Bioethanol and Biogas}, url = {https://m2.mtmt.hu/api/publication/33003548}, author = {Pilarski, Krzysztof and Pilarska, Agnieszka A. and Boniecki, Piotr and Niedbala, Gniewko and Witaszek, Kamil and Piekutowska, Magdalena and Idzior-Haufa, Malgorzata and Wawrzyniak, Agnieszka}, doi = {10.3390/en14227763}, journal-iso = {ENERGIES}, journal = {ENERGIES}, volume = {14}, unique-id = {33003548}, issn = {1996-1073}, abstract = {The integrated production of bioethanol and biogas makes it possible to optimise the production of carriers from renewable raw materials. The installation analysed in this experimental paper was a hybrid system, in which waste from the production of bioethanol was used in a biogas plant with a capacity of 1 MWe. The main objective of this study was to determine the energy potential of biomass used for the production of bioethanol and biogas. Based on the results obtained, the conversion rate of the biomass-maize, in this case-into bioethanol was determined as the efficiency of the process of bioethanol production. A biomass conversion study was conducted for 12 months, during which both maize grains and stillage were sampled once per quarter (QU-I, QU-II, QU-III, QU-IV; QU-quarter) for testing. Between 342 L (QU-II) and 370 L (QU-I) of ethanol was obtained from the organic matter subjected to alcoholic fermentation. The mass that did not undergo conversion to bioethanol ranged from 269.04 kg to 309.50 kg, which represented 32.07% to 36.95% of the organic matter that was subjected to the process of bioethanol production. On that basis, it was concluded that only two-thirds of the organic matter was converted into bioethanol. The remaining part-post-production waste in the form of stillage-became a valuable raw material for the production of biogas, containing one-third of the biodegradable fraction. Under laboratory conditions, between 30.5 m(3) (QU-I) and 35.6 m(3) (QU-II) of biogas per 1 Mg of FM (FM-fresh matter) was obtained, while under operating conditions, between 29.2 m(3) (QU-I) and 33.2 m(3) (QU-II) of biogas was acquired from 1 Mg of FM. The Biochemical Methane Potential Correction Coefficient (BMPCC), which was calculated based on the authors' formula, ranged from 3.2% to 7.4% in the analysed biogas installation.}, keywords = {Biogas; Bioethanol; Stillage; biomass conversion degree; biochemical methane potential correction coefficient (BMPCC)}, year = {2021}, eissn = {1996-1073}, orcid-numbers = {Pilarski, Krzysztof/0000-0003-0810-8086; Boniecki, Piotr/0000-0002-9239-4072; Witaszek, Kamil/0000-0002-8897-6459; Piekutowska, Magdalena/0000-0002-5301-4719; Wawrzyniak, Agnieszka/0000-0002-7949-7560} } @article{MTMT:31059579, title = {An overview of integration opportunities for sustainable bioethanol production from first- and second-generation sugar-based feedstocks}, url = {https://m2.mtmt.hu/api/publication/31059579}, author = {Ayodele, Bamidele Victor and Alsaffar, May Ali and Mustapa, Siti Indati}, doi = {10.1016/j.jclepro.2019.118857}, journal-iso = {J CLEAN PROD}, journal = {JOURNAL OF CLEANER PRODUCTION}, volume = {245}, unique-id = {31059579}, issn = {0959-6526}, abstract = {The production of bioethanol from second generation feedstocks which are mainly lignocellulosic biomass provides the opportunities for a cleaner, and low carbon biofuel that can serve as an alternative to fossil fuel. However, one major constrain is the high cost of production due to expensive pre-treatment technologies. On the other hand, bioethanol production using first generation feedstock is a proven and well-established technology with high bioethanol productivity and yield, but the process is identified with the issues of food-to-fuel debate and high environmental impact from land use charge. Integrating second generation bioethanol production process with the well proven first generation bioethanol production facilities has the potential of harnessing the synergistic effect that could maximize the technical, economic and environmental benefits of the integrated first and second generation process. This study examined literature on studies related to first generation bioethanol production, second generation bioethanol production and the integrated first and second generation processes with the aim of identifying the extent of integration opportunities for first and second generation bioethanol production from different sugar-based feedstocks. The review put into consideration the different process scenarios available in terms of the technical, economic and environmental benefits of the bioethanol production processes. The benefits of the different integration scenarios for bioethanol production were considered in comparison with the standalone first generation and second generation bioethanol production scenarios. Based on the consideration of the different integration scenarios, some recommendations were proposed for attention in future research on the integration of first and second generation bioethanol production from sugar-based feedstocks. (C) 2019 Elsevier Ltd. All rights reserved.}, keywords = {Bioethanol; Sugarcane; Second generation; First generation; Integration opportunity}, year = {2020}, eissn = {1879-1786} } @article{MTMT:31701994, title = {Predictive Modelling of Sugar Release from Blended Garden Wastes in a Microwave-Assisted Hot Water Process}, url = {https://m2.mtmt.hu/api/publication/31701994}, author = {Bai, Ruxue and Wang, Wen and Yu, Qiang and Zhang, Qinghua and Kong, Xiaoying and Sun, Yongming and Zhuang, Xinshu and Wang, Zhongming and Yuan, Zhenhong}, doi = {10.1007/s12649-019-00932-2}, journal-iso = {WASTE BIOMASS VALORI}, journal = {WASTE AND BIOMASS VALORIZATION}, unique-id = {31701994}, issn = {1877-2641}, abstract = {Due to supply difficulties caused by seasonal availability, fuel ethanol production from a single lignocellulosic biomass species is always uneconomical at commercial scale. The utilization of blended biomass offers a potential solution to this problem. In this study, a prediction model was developed to evaluate the sugar release from blended garden wastes in a microwave-assisted hot water (MHW) process. The optimum blending ratio ofBauhinia blakeana Dunn(BB), rice straw (RS) and sugarcane bagasse (SC) was found to be 2:3:5, which promised a high total xylose yield of 67.82% (using a process temperature of 186 degrees C for 43 min). While the yield of xylose could only reach 52.47% when adopting the single garden waste of BB as feedstock under the same condition. Furthermore, the use of blended materials provided a cost savings of 22.23% as compared with the single RS feedstock, and no significant difference was found in the release of total xylose. The model developed was found to accurately predict the total xylose released from blended garden feedstocks. In addition, a steady supply of lignocellulosic biomass for fuel ethanol production could also be generated.[GRAPHICS].}, keywords = {PRETREATMENT; Garden waste; Microwave-assisted hot water; Blending biomass}, year = {2020}, eissn = {1877-265X} } @article{MTMT:31701989, title = {Assessing the aggregated environmental benefits from by-product and utility synergies in the Swedish biofuel industry}, url = {https://m2.mtmt.hu/api/publication/31701989}, author = {Martin, Michael and Wetterlund, Elisabeth and Hackl, Roman and Holmgren, Kristina M. and Peck, Philip}, doi = {10.1080/17597269.2017.1387752}, journal-iso = {BIOFUELS-UK}, journal = {BIOFUELS}, volume = {11}, unique-id = {31701989}, issn = {1759-7269}, abstract = {The production of biofuels in Sweden has increased significantly in the past years in order to reduce fossil fuel dependence and mitigate climate impacts. Nonetheless, current methodological guidelines for assessing the GHG savings from the use of biofuels do not fully account for benefits from by-products and other utilities (e.g. waste heat and electricity) from biofuel production. This study therefore reviews the aggregated environmental performance of these multi-functional biofuel systems by assessing impacts and benefits from relevant production processes in Sweden in order to improve the decision base for biofuel producers and policymakers in the transition to a bio-based and circular economy. This was done by (1) conducting a mapping of the Swedish biofuel production portfolio, (2) developing future production scenarios, and (3) application of life cycle assessment methodology to assess the environmental performance of the production processes. Special focus was provided to review the potential benefits from replacing conventional products and services with by-products and utilities. The results provide evidence that failure to account for non-fuel-related benefits from biofuel production leads to an underestimation of the contribution of biofuels to reduce greenhouse gas emissions and other environmental impacts when replacing fossil fuels, showing the importance of their multi-functionality.}, keywords = {BENEFITS; Biofuels; LCA; SCENARIOS; BY-PRODUCTS; Industrial Symbiosis; consequential}, year = {2020}, eissn = {1759-7277}, pages = {683-698}, orcid-numbers = {Martin, Michael/0000-0003-3014-8930; Wetterlund, Elisabeth/0000-0002-4597-4082} } @article{MTMT:31701990, title = {A strategy for synergistic ethanol yield and improved production predictability through blending feedstocks}, url = {https://m2.mtmt.hu/api/publication/31701990}, author = {Persson, Michael and Galbe, Mats and Wallberg, Ola}, doi = {10.1186/s13068-020-01791-z}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {13}, unique-id = {31701990}, issn = {1754-6834}, abstract = {Background The integration of first- and second-generation bioethanol processes has the potential to accelerate the establishment of second-generation bioethanol on the market. Cofermenting pretreated wheat straw with a glucose-rich process stream, such as wheat grain hydrolysate, in a simultaneous saccharification and fermentation process could address the technical issues faced during the biological conversion of lignocellulose to ethanol. For example, doing so can increase the final ethanol concentration in the broth and mitigate the effects of inhibitors formed during the pretreatment. Previous research has indicated that blends of first- and second-generation substrates during simultaneous saccharification and fermentation have synergistic effects on the final ethanol yield, an important parameter in the process economy. In this study, enzymatic hydrolysis and simultaneous saccharification and fermentation were examined using blends of pretreated wheat straw and saccharified wheat grain at various ratios. The aim of this study was to determine the underlying mechanisms of the synergy of blending with regard to the yield and volumetric productivity of ethanol. Results Replacing 25% of the pretreated wheat straw with wheat grain hydrolysate during simultaneous saccharification and fermentation was sufficient to decrease the residence time needed to deplete soluble glucose from 96 to 24 h and shift the rate-limiting step from ethanol production to the rate of enzymatic hydrolysis. Further, a synergistic effect on ethanol yield was observed with blended substrates, coinciding with lower glycerol production. Also, blending substrates had no effect on the yield of enzymatic hydrolysis. Conclusions The effects of substrate blending on the volumetric productivity of ethanol were attributed to changes in the relative rates of cell growth and cell death due to alterations in the concentrations of substrate and pretreatment-derived inhibitors. The synergistic effect of substrate blending on ethanol yield was attributed in part to the decreased production of cell mass and glycerol. Thus, it is preferable to perform simultaneous saccharification and fermentation with substrate blends rather than pure substrates with regard to yield, productivity, and the robustness of the process.}, keywords = {HYDROLYSIS; Ethanol; FERMENTATION; Process integration; SSF; Substrate blending; Pretreated wheat straw; Saccharified wheat grain; Blending synergy; Fermentation dynamics}, year = {2020}, eissn = {1754-6834}, orcid-numbers = {Persson, Michael/0000-0001-8655-647X} } @article{MTMT:31448480, title = {Analysis of Animal Bedding Heterogeneity for Potential Use in Biorefineries Based on Farmyard Manure}, url = {https://m2.mtmt.hu/api/publication/31448480}, author = {Sanchis-Sebastia, Miguel and Erdei, Borbala and Kovacs, Krisztina and Galbe, Mats and Wallberg, Ola}, doi = {10.1007/s12649-018-00578-6}, journal-iso = {WASTE BIOMASS VALORI}, journal = {WASTE AND BIOMASS VALORIZATION}, volume = {11}, unique-id = {31448480}, issn = {1877-2641}, abstract = {Purpose Animal bedding, a mixture of straw and manure, could be used as a feedstock for bioenergy production, but its heterogeneity poses new challenges in its use in biorefineries. We have investigated the origin of this heterogeneity and quantified it, and discuss its impact on bioenergy production. Methods Samples were collected from the bedding at different points and depths and analysed by first separating the manure from the straw by means of washing with water, and then determining the chemical composition of the liquid and solid fractions. Results The results supported our hypothesis that animal bedding behaves as a combination of several layers at different stages of degradation. Analysis revealed that the layers with higher organic content in the manure exhibited a poorer performance during the washing, since the residence time in the barn alters the washing profile of the organic fraction in the manure. It was also found that the variability in the composition of animal bedding was much greater than in other agricultural feedstocks: the manure content in animal bedding varied from 26 to 41%, and the content of fermentable carbohydrates varied by 20%. Total carbon and total nitrogen analyses showed that these changes in composition also affected the C/N ratio of the material, and thus its suitability as a feedstock for anaerobic digestion. Conclusions This implies that the residence time in the barn affects not only the heterogeneity of the properties of animal bedding, but also the best way to process it in a biorefinery.}, keywords = {Bioenergy; MANURE; Compositional analysis; Animal bedding}, year = {2020}, eissn = {1877-265X}, pages = {2387-2395}, orcid-numbers = {Galbe, Mats/0000-0003-3290-6467; Wallberg, Ola/0000-0001-5697-1277} } @article{MTMT:31448479, title = {Assessment of the feedstock supply for siting single- and multiple-feedstock biorefineries in the USA and identification of prevalent feedstocks}, url = {https://m2.mtmt.hu/api/publication/31448479}, author = {Sharma, Bhavna and Brandt, Craig and McCullough-Amal, Devita and Langholtz, Matthew and Webb, Erin}, doi = {10.1002/bbb.2091}, journal-iso = {BIOFUEL BIOPROD BIOR}, journal = {BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR}, volume = {14}, unique-id = {31448479}, issn = {1932-104X}, abstract = {An integrated multi-feedstock bioenergy (i.e., biofuel, biopower, or bioproduct) supply system has potential to reduce biomass supply system uncertainties and costs. This study identifies optimal configurations of multi-feedstock biomass-to-biorefinery supply chains and pertinent feedstock combinations based on spatial distribution of feedstock and lowest delivered cost to the biorefinery. We used the Supply Characterization Model (SCM) to allocate feedstock supplies to candidate biorefinery facilities. Model runs were performed for herbaceous energy crops, agriculture residue, and woody biomass available in 2017, 2022, 2025, and 2030 as estimated by the Policy Analysis System (POLYSYS) and Forest Sustainable and Economic Analysis Model (ForSEAM) models. Three feedstock supply scenarios were compared: (a) an herbaceous scenario: switchgrass, miscanthus, biosorghum, and corn stover; (b) a woody scenario: coppice wood, noncoppice wood, whole trees, and forestry residues, and (c) a mixed scenario: a combination of all feedstocks in herbaceous and woody scenarios. By 2030 the analyses predicted that 323, 168, and 473 biorefineries were sited in the herbaceous, woody, and mixed scenario, respectively, in the conterminous USA. Feedstock mixes supplied to the biorefineries were mostly dominated by a single feedstock. The most prominent feedstock mixes identified were: (1) switchgrass and miscanthus; (2) coppice and noncoppice wood; and (3) coppice wood, noncoppice wood, switchgrass and miscanthus. Biorefineries using multi-feedstock would be beneficial for growth of bioeconomy, however flexible and cost-effective conversion platforms should be developed to efficiently utilize multiple feedstocks. This analysis identifies biorefinery locations and feedstock supply mixes while minimizing delivered feedstock costs based on spatial and temporal feedstock availability. (c) 2020 Society of Chemical Industry and John Wiley & Sons, Ltd}, keywords = {Modeling; spatial analysis; Feedstock; Biorefinery; herbaceous; woody}, year = {2020}, eissn = {1932-1031}, pages = {578-593}, orcid-numbers = {Langholtz, Matthew/0000-0002-8153-7154} } @article{MTMT:31448478, title = {Tracking strategic developments for conferring xylose utilization/fermentation bySaccharomyces cerevisiae}, url = {https://m2.mtmt.hu/api/publication/31448478}, author = {Sharma, Shalley and Arora, Anju}, doi = {10.1186/s13213-020-01590-9}, journal-iso = {ANN MICROBIOL}, journal = {ANNALS OF MICROBIOLOGY}, volume = {70}, unique-id = {31448478}, issn = {1590-4261}, abstract = {Purpose Efficient ethanol production through lignocellulosic biomass hydrolysates could solve energy crisis as it is economically sustainable and ecofriendly.Saccharomyces cerevisiaeis the work horse for lignocellulosic bioethanol production at industrial level. But its inability to ferment and utilize xylose limits the overall efficacy of the process. Method Data for the review was selected using different sources, such as Biofuels digest, Statista, International energy agency (IEA). Google scholar was used as a search engine to search literature for yeast metabolic engineering approaches. Keywords used were metabolic engineering of yeast for bioethanol production from lignocellulosic biomass. Result Through these approaches, interconnected pathways can be targeted randomly. Moreover, the improved strains genetic makeup can help us understand the mechanisms involved for this purpose. Conclusion This review discusses all possible approaches for metabolic engineering of yeast. These approaches may reveal unknown hidden mechanisms and construct ways for the researchers to produce novel and modified strains.}, keywords = {ADAPTATION; Saccharomyces cerevisiae; metabolic engineering; PROTOPLAST FUSION; Random approaches}, year = {2020}, eissn = {1869-2044} } @article{MTMT:31423583, title = {Saccharification and fermentation of pretreated banana leaf waste for ethanol production}, url = {https://m2.mtmt.hu/api/publication/31423583}, author = {Suhag, Meenakshi and Kumar, Anil and Singh, Joginder}, doi = {10.1007/s42452-020-03215-x}, journal-iso = {SN APPL SCI}, journal = {SN APPLIED SCIENCES}, volume = {2}, unique-id = {31423583}, issn = {2523-3963}, abstract = {The present work reveals the potential applicability of banana leaf waste as feedstock for bioethanol production as very less work has been reported on this feedstock. For achieving the same, the performance of crude cellulases of Aspergillus niger JD-11 is explored for saccharification of untreated and pretreated (alkaline, dilute acid and steam) banana leaf waste. Therefore, to determine the best saccharifying conditions, the effects of various parameters such as enzyme loading (5-15 FPU/g), temperature (40-50 degrees C), surfactant addition (0.05-0.15% Tween 80 and PEG 6000) and substrate concentrations (2-6%) were studied at 150 rpm and pH 5.0. The crude cellulases yielded maximum reducing sugars (524.83 mg/g) from the dilute acid pretreated banana leaf waste at 45 degrees C, 15 FPU/g enzyme loading and 2% substrate loading in the presence of 0.15% PEG 6000 (w/v) as surfactant. The fermentation of enzymatic hydrolysate was carried out using initial reducing sugars concentration of 40 g/L in the medium at pH 5.5, 150 rpm and 30 degrees C for 30 h with the help of Saccharomyces cerevisiae. Ethanol production was estimated at every 6 h and maximum ethanol yield of 0.38 g/g sugar and productivity of 1.28 g/L/h was obtained after 12 h of fermentation of banana leaf waste hydrolysate. Thus, the easily obtained banana leaf waste could be a promising feedstock for bioethanol production.}, keywords = {Ethanol; FERMENTATION; PRETREATMENT; SACCHARIFICATION; Cellulases; reducing sugars; Banana leaf waste; Surfactants (PEG 6000 and Tween 80)}, year = {2020}, eissn = {2523-3971} } @article{MTMT:31448477, title = {Overexpression of an endogenous raw starch digesting mesophilic alpha-amylase gene in Bacillus amyloliquefaciens Z3 by in vitro methylation protocol}, url = {https://m2.mtmt.hu/api/publication/31448477}, author = {Tang, Shizhe and Xu, Tingliang and Peng, Jing and Zhou, Kaiyan and Zhu, Yuling and Zhou, Wenbo and Cheng, Haina and Zhou, Hongbo}, doi = {10.1002/jsfa.10332}, journal-iso = {J SCI FOOD AGR}, journal = {JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE}, volume = {100}, unique-id = {31448477}, issn = {0022-5142}, abstract = {BACKGROUND Mesophilic alpha-amylases function effectively at low temperatures with high rates of catalysis and require less energy for starch hydrolysis. Bacillus amyloliquefaciens is an essential producer of mesophilic alpha-amylases. However, because of the existence of the restriction-modification system, introducing exogenous DNAs into wild-type B. amyloliquefaciens is especially tricky.RESULTS alpha-Amylase producer B. amyloliquefaciens strain Z3 was screened and used as host for endogenous alpha-amylase gene expression. In vitro methylation was performed in recombinant plasmid pWB980-amyZ3. With the in vitro methylation, the transformation efficiency was increased to 0.96 x 10(2) colony-forming units mu g(-1) plasmid DNA. A positive transformant BAZ3-16 with the highest alpha-amylase secreting capacity was chosen for further experiments. The alpha-amylase activity of strain BAZ3-16 reached 288.70 +/- 16.15 U mL(-1) in the flask and 386.03 +/- 16.25 U mL(-1) in the 5-L stirred-tank fermenter, respectively. The Bacillus amyloliquefaciens Z3 expression system shows excellent genetic stability and high-level extracellular production of the target protein. Moreover, the synergistic interaction of AmyZ3 with amyloglucosidase was determined during the hydrolysis of raw starch. The hydrolysis degree reached 92.34 +/- 3.41% for 100 g L-1 raw corn starch and 81.30 +/- 2.92% for 100 g L-1 raw cassava starch after 24 h, respectively.CONCLUSION Methylation of the plasmid DNA removes a substantial barrier for transformation of B. amyloliquefaciens strain Z3. Furthermore, the exceptional ability to hydrolyze starch makes alpha-amylase AmyZ3 and strain BAZ3-16 valuable in the starch industry. (c) 2020 Society of Chemical Industry}, keywords = {Genetic engineering; restriction-modification system; Bacillus amyloliquefaciens; mesophilic alpha-amylase; industrial strain; raw starch hydrolysis}, year = {2020}, eissn = {1097-0010}, pages = {3013-3023} } @article{MTMT:31701992, title = {Production of Cellulosic Ethanol from Enzymatically Hydrolysed Wheat Straws}, url = {https://m2.mtmt.hu/api/publication/31701992}, author = {Ursachi, Vasile-Florin and Gutt, Gheorghe}, doi = {10.3390/app10217638}, journal-iso = {APPL SCI-BASEL}, journal = {APPLIED SCIENCES-BASEL}, volume = {10}, unique-id = {31701992}, abstract = {The aim of this study is to find the optimal pretreatment conditions and hydrolysis in order to obtain a high yield of bioethanol from wheat straw. The pretreatments were performed with different concentrations of sulphuric acid 1, 2 and 3% (v/v), and were followed by an enzymatic hydrolysis that was performed by varying the solid-to-liquid ratio (1/20, 1/25 and 1/30 g/mL) and the enzyme dose (30/30 mu L/g, 60/60 mu L/g and 90/90 mu L/g Viscozyme(R) L/Celluclast(R) 1.5 L). This mix of enzymes was used for the first time in the hydrolysis process of wheat straws which was previously pretreated with dilute sulfuric acid. Scanning electron microscopy indicated significant differences in the structural composition of the samples because of the pretreatment with H2SO4 at different concentrations, and ATR-FTIR analysis highlighted the changes in the chemical composition in the pretreated wheat straw as compared to the untreated one. HPLC-RID was used to identify and quantify the carbohydrates content resulted from enzymatic hydrolysis to evaluate the potential of using wheat straws as a raw material for production of cellulosic ethanol in Romania. The highest degradation of lignocellulosic material was obtained in the case of pretreatment with 3% H2SO4 (v/v), a solid-to-liquid ratio of 1/30 and an enzyme dose of 90/90 mu L/g. Simultaneous saccharification and fermentation were performed using Saccharomyces cerevisiae yeast, and for monitoring the fermentation process a BlueSens equipment was used provided with ethanol, O-2 and CO2 cap sensors mounted on the fermentation flasks. The highest concentration of bioethanol was obtained after 48 h of fermentation and it reached 1.20% (v/v).}, keywords = {HYDROLYSIS; FERMENTATION; PRETREATMENT; Bioethanol; Wheat straws}, year = {2020}, eissn = {2076-3417} } @article{MTMT:31701995, title = {Development and characterization of acidic-pH-tolerant mutants of Zymomonas mobilis through adaptation and next-generation sequencing-based genome resequencing and RNA-Seq}, url = {https://m2.mtmt.hu/api/publication/31701995}, author = {Yang, Qing and Yang, Yongfu and Tang, Ying and Wang, Xia and Chen, Yunhao and Shen, Wei and Zhan, Yangyang and Gao, Junjie and Wu, Bo and He, Mingxiong and Chen, Shouwen and Yang, Shihui}, doi = {10.1186/s13068-020-01781-1}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {13}, unique-id = {31701995}, issn = {1754-6834}, abstract = {Background: Acid pretreatment is a common strategy used to break down the hemicellulose component of the lignocellulosic biomass to release pentoses, and a subsequent enzymatic hydrolysis step is usually applied to release hexoses from the cellulose. The hydrolysate after pretreatment and enzymatic hydrolysis containing both hexoses and pentoses can then be used as substrates for biochemical production. However, the acid-pretreated liquor can also be directly used as the substrate for microbial fermentation, which has an acidic pH and contains inhibitory compounds generated during pretreatment. Although the natural ethanologenic bacteriumZymomonas mobiliscan grow in a broad range of pH 3.5 similar to 7.5, cell growth and ethanol fermentation are still affected under acidic-pH conditions below pH 4.0.Results: In this study, adaptive laboratory evolution (ALE) strategy was applied to adaptZ. mobilisunder acidic-pH conditions. Two mutant strains named 3.6M and 3.5M with enhanced acidic pH tolerance were selected and confirmed, of which 3.5M grew better than ZM4 but worse than 3.6M in acidic-pH conditions that is served as a reference strain between 3.6M and ZM4 to help unravel the acidic-pH tolerance mechanism. Mutant strains 3.5M and 3.6M exhibited 50 similar to 130% enhancement on growth rate, 4 similar to 9 h reduction on fermentation time to consume glucose, and 20 similar to 63% improvement on ethanol productivity than wild-type ZM4 at pH 3.8. Next-generation sequencing (NGS)-based whole-genome resequencing (WGR) and RNA-Seq technologies were applied to unravel the acidic-pH tolerance mechanism of mutant strains. WGR result indicated that compared to wild-type ZM4, 3.5M and 3.6M have seven and five single nucleotide polymorphisms (SNPs), respectively, among which four are shared in common. Additionally, RNA-Seq result showed that the upregulation of genes involved in glycolysis and the downregulation of flagellar and mobility related genes would help generate and redistribute cellular energy to resist acidic pH while keeping normal biological processes inZ. mobilis. Moreover, genes involved in RND efflux pump, ATP-binding cassette (ABC) transporter, proton consumption, and alkaline metabolite production were significantly upregulated in mutants under the acidic-pH condition compared with ZM4, which could help maintain the pH homeostasis in mutant strains for acidic-pH resistance. Furthermore, our results demonstrated that in mutant 3.6M, genes encoding F(1)F(0)ATPase to pump excess protons out of cells were upregulated under pH 3.8 compared to pH 6.2. This difference might help mutant 3.6M manage acidic conditions better than ZM4 and 3.5M. A few gene targets were then selected for genetics study to explore their role in acidic pH tolerance, and our results demonstrated that the expression of two operons in the shuttle plasmids,ZMO0956-ZMO0958encoding cytochrome bc1 complex andZMO1428-ZMO1432encoding RND efflux pump, could helpZ. mobilistolerate acidic-pH conditions.Conclusion: An acidic-pH-tolerant mutant 3.6M obtained through this study can be used for commercial bioethanol production under acidic fermentation conditions. In addition, the molecular mechanism of acidic pH tolerance ofZ. mobiliswas further proposed, which can facilitate future research on rational design of synthetic microorganisms with enhanced tolerance against acidic-pH conditions. Moreover, the strategy developed in this study combining approaches of ALE, genome resequencing, RNA-Seq, and classical genetics study for mutant evolution and characterization can be applied in other industrial microorganisms.}, keywords = {RNA-Seq; next-generation sequencing (NGS); Zymomonas mobilis; Adaptive laboratory evolution (ALE); Acidic pH tolerance; Whole-genome resequencing (WGR)}, year = {2020}, eissn = {1754-6834} } @article{MTMT:31450033, title = {Characterization of an alkali-tolerant, thermostable, and multifunctional GH5 family endoglucanase fromThermoactinospora rubraYIM 77501(T)for prebiotic production}, url = {https://m2.mtmt.hu/api/publication/31450033}, author = {Yin, Yi-Rui and Li, Tao and Sang, Peng and Yang, Run-Fen and Liu, Hong-Yan and Xiao, Min and Li, Shuai and Yang, Li-Quan and Li, Wen-Jun}, doi = {10.1007/s13399-020-00848-1}, journal-iso = {BIOMASS CONV BIOREFINERY}, journal = {BIOMASS CONVERSION AND BIOREFINERY}, unique-id = {31450033}, issn = {2190-6815}, abstract = {A novel endoglucanase gene (1425 bp), designatedthrcel5A, was cloned fromThermoactinospora rubraYIM 77501(T)and determined to be a member of glycoside hydrolase family 5. The putative amino acid sequence displayed 76% conservation with reported endoglucanases (GenBank: SCG57304.1) fromMicromonospora siamensis.Thrcel5Awas expressed inEscherichia coliBL 21 (DE3) and purified using Ni2+-affinity chromatography. The resulting purified protein displayed high hydrolytic activity against the sodium salt of carboxymethyl cellulose and beta-(1, 3; 1, 4)-glucans from barley and beechwood xylan, with specific activities of 85.7 +/- 1.5, 120.3 +/- 2.6, and 22.9 +/- 1.1 U/mg, respectively. The optimal pH and temperature for the recombinant enzyme were determined to be 8.5 and 60 degrees C, respectively. Additionally, ThrCel5A was thermotolerant as it retained more than 60% of its original activity after an incubation at 60 degrees C for 2 h. Moreover, ThrCel5A can hydrolyze beta-(1, 3; 1, 4)-glucan into prebiotics, such as cellobiose, cellotriose, and cellotetrose. Its endoglucanase activity was significantly affected by link sequences and CBM2. Due to being an alkali-tolerant, thermostable, and multifunctional cellulolytic enzyme, ThrCel5A is an attractive candidate for use in production of prebiotics.}, keywords = {THERMOSTABLE; Thermoactinospora rubraYIM 77501(T); Alkali-tolerant; Multifunctional endoglucanase}, year = {2020}, eissn = {2190-6823} } @article{MTMT:31573268, title = {The development of a biorefining strategy for the production of biofuel from sorghum milling waste}, url = {https://m2.mtmt.hu/api/publication/31573268}, author = {El-Imam, Amina M. Ahmed and Greetham, Darren and Du, Chenyu and Dyer, Paul S.}, doi = {10.1016/j.bej.2019.107288}, journal-iso = {BIOCHEM ENG J}, journal = {BIOCHEMICAL ENGINEERING JOURNAL}, volume = {150}, unique-id = {31573268}, issn = {1369-703X}, abstract = {The potential of using sorghum milling waste for the development of a biorefining strategy for the production of bioethanol was investigated. Both red and white sorghum were processed using a traditional Nigerian wetmilling process to sorghum flour. The sorghum milling waste, sorghum bran, was hydrolysed using both enzymatic and dilute acid hydrolysis to produce a generable fermentation feedstock. The hydrolysates were subsequently investigated for fermentative biofuel production. Following a hydrolysis step, a medium containing (similar to)61 g/L glucose was obtained. Trace presence of inhibitors was detected in the hydrolysates and sufficient nitrogen content to support microorganism growth and bioethanol production. In test bioethanol production experiments using the sorghum milling waste derived hydrolysates only, 24.35 g/L bioethanol was produced by a yeast Kluyveromyces marxianus, equivalent to a yield of 0.15 g bioethanol per g of sorghum milling waste.}, keywords = {HYDROLYSIS; Bioethanol; Biorefinery; Sorghum bran; sorghum milling waste; Yeast screening}, year = {2019}, eissn = {1873-295X} } @article{MTMT:31058008, title = {Enhanced co-generation of cellulosic ethanol and methane with the starch/sugar-rich waste mixtures and Tween 80 in fed-batch mode}, url = {https://m2.mtmt.hu/api/publication/31058008}, author = {Fan, Meishan and Li, Jun and Bi, Guican and Ye, Guangying and Zhang, Hongdan and Xie, Jun}, doi = {10.1186/s13068-019-1562-0}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {12}, unique-id = {31058008}, issn = {1754-6834}, abstract = {Background The mixed-feedstock fermentation is a promising approach to enhancing the co-generation of cellulosic ethanol and methane from sugarcane bagasse (SCB) and molasses. However, the unmatched supply of the SCB and molasses remains a main obstacle built upon binary feedstock. Here, we propose a cellulose-starch-sugar ternary waste combinatory approach to overcome this bottleneck by integrating the starch-rich waste of Dioscorea composita Hemls. extracted residue (DER) in mixed fermentation. Results The substrates of the pretreated SCB, DER and molasses with varying ratios were conducted at a relatively low solids loading of 12%, and the optimal mixture ratio of 1:0.5:0.5 for the pretreated SCB/DER/molasses was determined by evaluating the ethanol concentration and yield. Nevertheless, it was found that the ethanol yield decreased from 79.19 +/- 0.20 to 62.31 +/- 0.61% when the solids loading increased from 12 to 44% in batch modes, regardless of the fact that the co-fermentation of three-component feedstock was performed under the optimal condition defined above. Hence, different fermentation processes such as fed-batch and fed-batch + Tween 80 were implemented to further improve the ethanol concentration and yield at higher solids loading ranging between 36 and 44%. The highest ethanol concentration of 91.82 +/- 0.86 g/L (69.33 +/- 0.46% of theoretical yield) was obtained with fed-batch + Tween 80 mode during the simultaneous saccharification and fermentation at a high solids loading of 44%. Moreover, after the ethanol recovery, the remaining stillage was digested for biomethane production and finally yielded 320.72 +/- 6.98 mL/g of volatile solids. Conclusions Integrated DER into the combination of SCB and molasses would be beneficial for ethanol production. The co-generation of bioethanol and biomethane by mixed cellulose-starch-sugar waste turns out to be a sustainable solution to improve the overall efficacy in biorefinery.}, keywords = {Biomethane; Bioethanol; Sugarcane bagasse; high solids loading; Cellulose-starch-sugar waste; Fed batch}, year = {2019}, eissn = {1754-6834} } @article{MTMT:31059584, title = {Enzymatic hydrolysis of hemicellulose from pretreated Finger millet Check for (Eleusine coracana) straw by recombinant endo-1,4-beta-xylanase updates and exo-1.4-beta-xylosidase}, url = {https://m2.mtmt.hu/api/publication/31059584}, author = {Jamaldheen, Sumitha Banu and Thakur, Abhijeet and Moholkar, Vijayanand S. and Goyal, Arun}, doi = {10.1016/j.ijbiomac.2019.06.010}, journal-iso = {INT J BIOL MACROMOL}, journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES}, volume = {135}, unique-id = {31059584}, issn = {0141-8130}, abstract = {This study focuses on enzymatic saccharification of hemicellulose part of the pretreated Finger millet straw (FMS) for production of xylose. The variation in the carbohydrate composition of FMS was analysed when subjected to different pretreatments. The recombinant endo-1.4-beta-xylanase (CtXynl 1A) was most active on the FMS pretreated with 1% (w/v) NaOH combined with oven heating at 120 degrees C for 20 min, resulting in a total reducing sugar yield (TRS) of 32 mg/g pretreated biomass. The pretreatment aided in concentrating the holocellulose content from 69.3% of raw powdered FMS to 76.4%. The post-treatment solid biomass yield was 0.36 g/g raw biomass. The two-step optimization of hemicellulose saccharification from the above pretreated FMS with i) endo1.4-beta-xylanase (CtXyn11A) at 55 degrees C and ii) exo-1.4-beta-xylosidase (BoGH43A) at 37 degrees C, both at pH 7.5 by BoxBehnken design yielded the TRS of 70 mg/g pretreated biomass. The percentage conversion of xylan to xylose by CtXynl IA and BoGH43A was 24.7%. (C) 2019 Elsevier B.V. All rights reserved.}, keywords = {recombinant enzymes; Finger millet straw; Xylan conversion}, year = {2019}, eissn = {1879-0003}, pages = {1098-1106} } @article{MTMT:31059580, title = {Hydrotreatment of pyrolysis liquids derived from second-generation bioethanol production residues over NiMo and CoMo catalysts}, url = {https://m2.mtmt.hu/api/publication/31059580}, author = {Priharto, Neil and Ronsse, Frederik and Prins, Wolter and Hita, Idoia and Deuss, Peter J. and Heeres, Hero Jan}, doi = {10.1016/j.biombioe.2019.05.005}, journal-iso = {BIOMASS BIOENERGY}, journal = {BIOMASS & BIOENERGY}, volume = {126}, unique-id = {31059580}, issn = {0961-9534}, abstract = {Lignin-rich digested stillage from second-generation bioethanol production is a unique biomass-derived feedstock, not only because it contains high amounts of lignin but also due to its residual amounts of cellulose and hemicellulose. In this study, catalytic hydrotreatment experiments were conducted on pyrolysis liquids obtained from the lignin-rich feedstock using sulphided NiMo/Al2O3 and CoMo/Al2O3 catalysts. The aim was to obtain a high conversion of the initial pyrolysis feed into a hydrotreated oil with a high phenolics and aromatics fractions. Experiments were carried out in a stirred batch reactor at 350 degrees C and 10 MPa of H-2 (initial pressure). Product oils were obtained in about 60-65% w/w, the remainder being an aqueous phase (12-14% w/w), solids (7-8% w/w) and gas phase components (all on initial pyrolysis oil feed basis). The product oils were characterised in detail using various techniques (elemental composition, GCxGC-FID, GPC, and 2D HSQC NMR). The oxygen content was reduced from 23% w/w in the pyrolysis oils to 7.5-11.5% in the hydrotreated oils, indicative of the occurrence of hydrodeoxygenation reactions. This was also evident from the chemical composition, showing an increase in the amounts of low molecular weight aromatics, alkylphenolics, alkanes and cycloalkanes in hydrotreated oils. Performance of the two catalysts was compared, and a higher degree of deoxygenation was observed for the NiMo catalyst. The implications of the findings for the valorisation of second-generation bioethanol residues are also discussed.}, keywords = {Biobased chemicals; Hydrotreatment; Lignin-rich digested stillage; Pyrolysis liquids; Sulfided catalysts}, year = {2019}, eissn = {1873-2909}, pages = {84-93}, orcid-numbers = {Ronsse, Frederik/0000-0002-3290-9177} } @article{MTMT:31059583, title = {Introducing low-quality feedstocks in bioethanol production: efficient conversion of the lignocellulose fraction of animal bedding through steam pretreatment}, url = {https://m2.mtmt.hu/api/publication/31059583}, author = {Sanchis-Sebastia, Miguel and Erdei, Borbala and Kovacs, Krisztina and Galbe, Mats and WallbergE, Ola}, doi = {10.1186/s13068-019-1558-9}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {12}, unique-id = {31059583}, issn = {1754-6834}, abstract = {Background Animal bedding remains an underutilized source of raw material for bioethanol production, despite the economic and environmental benefits of its use. Further research concerning the optimization of the production process is needed, as previously tested pretreatment methods have not increased the conversion efficiency to the levels necessary for commercialization of the process. Results We propose steam pretreatment of animal bedding, consisting of a mixture of straw and cow manure, to deliver higher ethanol yields. The temperature, residence time and pH were optimized through response-surface modeling, where pretreatment was evaluated based on the ethanol yield obtained through simultaneous saccharification and fermentation of the whole pretreated slurry. The results show that the best conditions for steam pretreatment are 200 degrees C, for 5 min at pH 2, at which an ethanol yield of about 70% was obtained. Moreover, the model also showed that the pH had the greatest influence on the ethanol yield, followed by the temperature and then the residence time. Conclusions Based on these results, it appears that steam pretreatment could unlock the potential of animal bedding, as the same conversion efficiencies were achieved as for higher-quality feedstocks such as wheat straw.}, keywords = {Bioethanol; Animal bedding; Steam pretreatment; Response-surface modeling}, year = {2019}, eissn = {1754-6834} } @article{MTMT:30565436, title = {Process integration for ethanol production from corn and corn stover as mixed substrates}, url = {https://m2.mtmt.hu/api/publication/30565436}, author = {Yu, Jianming and Xu, Zhaoxian and Liu, Lei and Chen, Sitong and Wang, Shengwei and Jin, Mingjie}, doi = {10.1016/j.biortech.2019.01.112}, journal-iso = {BIORESOUR TECHNOL}, journal = {BIORESOURCE TECHNOLOGY}, volume = {279}, unique-id = {30565436}, issn = {0960-8524}, abstract = {This work investigated all possible process integration strategies for ethanol production from corn and dilute acid pretreated corn stover (CS) as mixed substrates. Three corn to pretreated CS ratios (20%: 10%, 10%: 20% and 5%: 25%) were examined. When the ratio of corn to pretreated CS was 20%: 10%, the process integration strategy that mixed corn with CS hydrolysate for liquefaction followed by SSF resulted in the highest ethanol titer of 99.3 g/L. Mixing liquefied corn with pretreated CS for hydrolysis/saccharification followed by fermentation was the best strategy for the other two ratios. The strategy of mixing liquefied corn with pretreated CS for 6 h hydrolysis followed by fermentation showed the highest productivity for all the tested ratios.}, keywords = {Cellulosic ethanol; Corn ethanol; Integrated ethanol production process; The first generation ethanol; The second generation ethanol}, year = {2019}, eissn = {1873-2976}, pages = {10-16} } @article{MTMT:27570770, title = {Crop diversity for mixed first and second generation ethanol production}, url = {https://m2.mtmt.hu/api/publication/27570770}, author = {Buck, Michael and Senn, Thomas}, doi = {10.1080/17597269.2016.1266233}, journal-iso = {BIOFUELS-UK}, journal = {BIOFUELS}, volume = {9}, unique-id = {27570770}, issn = {1759-7269}, year = {2018}, eissn = {1759-7277}, pages = {291-303} } @article{MTMT:27570762, title = {Integrated bioethanol production from mixtures of corn and corn stover}, url = {https://m2.mtmt.hu/api/publication/27570762}, author = {Chen, Sitong and Xu, Zhaoxian and Li, Xiujuan and Yu, Jianming and Cai, Mufeng and Jin, Mingjie}, doi = {10.1016/j.biortech.2018.02.125}, journal-iso = {BIORESOUR TECHNOL}, journal = {BIORESOURCE TECHNOLOGY}, volume = {258}, unique-id = {27570762}, issn = {0960-8524}, year = {2018}, eissn = {1873-2976}, pages = {18-25} } @article{MTMT:30565440, title = {PERFORMANCE AND KINETIC STUDY OF THE ANAEROBIC CO-DIGESTION OF COCOA HUSK AND DIGESTED COW MANURE WITH HIGH ORGANIC LOADING RATE}, url = {https://m2.mtmt.hu/api/publication/30565440}, author = {Darwin and Ilham, Muhammad and Fazil, Afrizal}, journal-iso = {INMATEH AGRICULT ENG}, journal = {INMATEH - AGRICULTURAL ENGINEERING}, volume = {55}, unique-id = {30565440}, issn = {2068-2239}, abstract = {Biogas is a clean and cheap renewable energy that can be used for generating electricity. One of the current methods applied to enhance biogas production was through anaerobic co-digestion. The current study revealed that anaerobic digestion of cow manure co-digested with cocoa husk produced higher biogas production (348.3 mL/day) than anaerobic digestion of cow manure alone (26.5 mL/day). Even if a high organic loading rate (OLR) (4.173 kg VS/m(3).day) was applied to the reactor of anaerobic co-digestion, no inhibition was found at which pH was stable at 7.08. The results suggested that high OLR and short HRT (10 days) did not inhibit biogas production.}, keywords = {Biogas production; ANAEROBIC CO-DIGESTION; cocoa husk; Short HRT}, year = {2018}, pages = {131-140} } @article{MTMT:30565439, title = {Integrating sugarcane molasses into sequential cellulosic biofuel production based on SSF process of high solid loading}, url = {https://m2.mtmt.hu/api/publication/30565439}, author = {Fan, Meishan and Zhang, Shuaishuai and Ye, Guangying and Zhang, Hongdan and Xie, Jun}, doi = {10.1186/s13068-018-1328-0}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {11}, unique-id = {30565439}, issn = {1754-6834}, abstract = {BackgroundSugarcane bagasse (SCB) is one of the most promising lignocellulosic biomasses for use in the production of biofuels. However, bioethanol production from pure SCB fermentation is still limited by its high process cost and low fermentation efficiency. Sugarcane molasses, as a carbohydrate-rich biomass, can provide fermentable sugars for ethanol production. Herein, to reduce high processing costs, molasses was integrated into lignocellulosic ethanol production in batch modes to improve the fermentation system and to boost the final ethanol concentration and yield.ResultsThe co-fermentation of pretreated SCB and molasses at ratios of 3:1 (mixture A) and 1:1 (mixture B) were conducted at solid loadings of 12% to 32%, and the fermentation of pretreated SCB alone at the same solid loading was also compared. At a solid loading of 32%, the ethanol concentrations of 64.10g/L, 74.69g/L, and 75.64g/L were obtained from pure SCB, mixture A, and mixture B, respectively. To further boost the ethanol concentration, the fermentation of mixture B (1:1), with higher solid loading from 36 to 48%, was also implemented. The highest ethanol concentration of 94.20g/L was generated at a high solid loading of 44%, with an ethanol yield of 72.37%. In addition, after evaporation, the wastewater could be converted to biogas by anaerobic digestion. The final methane production of 312.14mL/g volatile solids (VS) was obtained, and the final chemical oxygen demand removal and VS degradation efficiency was 85.9% and 95.9%, respectively.ConclusionsMolasses could provide a good environment for the growth of yeast and inoculum. Integrating sugarcane molasses into sequential cellulosic biofuel production could improve the utilization of biomass resources.}, keywords = {Biogas; Bioethanol; Sugarcane bagasse; High solid loading; Co-fermentation; Sugarcane molasses}, year = {2018}, eissn = {1754-6834} } @article{MTMT:30565438, title = {A review of integration strategies of lignocelluloses and other wastes in 1st generation bioethanol processes}, url = {https://m2.mtmt.hu/api/publication/30565438}, author = {Ferreira, Jorge A. and Brancoli, Pedro and Agnihotri, Swarnima and Bolton, Kim and Taherzadeh, Mohammad J.}, doi = {10.1016/j.procbio.2018.09.006}, journal-iso = {PROCESS BIOCHEM}, journal = {PROCESS BIOCHEMISTRY}, volume = {75}, unique-id = {30565438}, issn = {1359-5113}, abstract = {First-generation ethanol plants offer successful, commercial-scale bioprocesses that can, at least partially, replace fossil fuels. They can act as platforms to integrate lignocelluloses, wastes and residuals when establishing 2nd generation ethanol. The present review gathers recent insights on the integration of intrinsic and extrinsic substrates into lot generation ethanol plants, through microbial conversion or cogeneration systems. It shows that, among different lot generation ethanol plants, sugar-based ethanol by-products, dominate integration studies characterized by strong techno-economic and life-cycle assessment components. In comparison, there are fewer studies that focus on grain-derived lignocellulosic residuals and other wastes. There is consensus that integrating second generation feedstocks into first generation plants can have positive techno-economic and environmental impacts. In addition to realizing production of ethanol from 2nd generation feedstocks, these possibilities can impact waste management by establishing relevant biorefineries and circular economy. They can also supply a wide range of renewable products. Considering the potential of this waste management strategy, further research on these and many other substrates is needed. This will shed light on the effect of the integration, the relevant types of microorganisms and pretreatments, and of other physical parameters on the effectiveness of running lot generation plants with integrated second generation feedstocks.}, keywords = {Filamentous fungi; Wastes; Biorefinery; Lignocelluloses; 1st generation ethanol; 2nd dgeneration ethanol}, year = {2018}, eissn = {1873-3298}, pages = {173-186} } @article{MTMT:27593662, title = {Catalytic Transformation of Cellulose and Its Derivatives into Functionalized Organic Acids}, url = {https://m2.mtmt.hu/api/publication/27593662}, author = {Li, Shi and Deng, Weiping and Wang, Shanshan and Wang, Pan and An, Dongli and Li, Yanyun and Zhang, Qinghong and Wang, Ye}, doi = {10.1002/cssc.201800440}, journal-iso = {CHEMSUSCHEM}, journal = {CHEMSUSCHEM}, volume = {11}, unique-id = {27593662}, issn = {1864-5631}, year = {2018}, eissn = {1864-564X}, pages = {1995-2028}, orcid-numbers = {Deng, Weiping/0000-0002-9108-3651; Wang, Ye/0000-0003-0764-2279} } @article{MTMT:27570761, title = {Integrated bioethanol production from triticale grain and lignocellulosic straw in Western Canada}, url = {https://m2.mtmt.hu/api/publication/27570761}, author = {Mupondwa, Edmund and Li, Xue and Tabil, Lope}, doi = {10.1016/j.indcrop.2018.02.070}, journal-iso = {IND CROP PROD}, journal = {INDUSTRIAL CROPS AND PRODUCTS}, volume = {117}, unique-id = {27570761}, issn = {0926-6690}, year = {2018}, eissn = {1872-633X}, pages = {75-87} } @article{MTMT:30565437, title = {Process design and optimization of bioethanol production from cassava bagasse using statistical design and genetic algorithm}, url = {https://m2.mtmt.hu/api/publication/30565437}, author = {Sivamani, Selvaraju and Baskar, Rajoo}, doi = {10.1080/10826068.2018.1514512}, journal-iso = {PREP BIOCHEM BIOTECH}, journal = {PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY}, volume = {48}, unique-id = {30565437}, issn = {1082-6068}, abstract = {Bioethanol production from agro-industrial residues is gaining attention because of the limited production of starch grains and sugarcane, and food-fuel conflict. The aim of the present study is to maximize the bioethanol production using cassava bagasse as a feedstock. Enzymatic liquefaction, by alpha-amylase, followed by simultaneous saccharification and fermentation (SSF), using glucoamylase and Zymomonas mobilis MTCC 2427, was investigated for bioethanol production from cassava bagasse. The factors influencing ethanol production process were identified and screened for significant factors using Plackett-Burman design. The significant factors (cassava bagasse concentration (10-50 g/L), concentration of alpha-amylase (5-25% (v/v), and temperature of fermentation (27-37 degrees C)) were optimized by employing Box-Behnken design and genetic algorithm. The maximum ethanol concentrations of 25.594 g/L and 25.910 g/L were obtained from Box-Behnken design and genetic algorithm, respectively, under optimum conditions. Thus, the study provides valuable insights in utilizing the cost-effective industrial residue, cassava bagasse, for the bioethanol production.}, keywords = {Simultaneous saccharification and fermentation; Cassava bagasse; enzymatic liquefaction; statistical design}, year = {2018}, eissn = {1532-2297}, pages = {834-841} } @article{MTMT:27570765, title = {Integrated bioethanol production to boost low-concentrated cellulosic ethanol without sacrificing ethanol yield}, url = {https://m2.mtmt.hu/api/publication/27570765}, author = {Xu, Youjie and Zhang, Meng and Roozeboom, Kraig and Wang, Donghai}, doi = {10.1016/j.biortech.2017.11.056}, journal-iso = {BIORESOUR TECHNOL}, journal = {BIORESOURCE TECHNOLOGY}, volume = {250}, unique-id = {27570765}, issn = {0960-8524}, year = {2018}, eissn = {1873-2976}, pages = {299-305} } @article{MTMT:27600332, title = {Ethanol production from mixtures of sugarcane bagasse and Dioscorea composita extracted residue with high solid loading}, url = {https://m2.mtmt.hu/api/publication/27600332}, author = {Ye, Guangying and Zeng, Defu and Zhang, Shuaishuai and Fan, Meishan and Zhang, Hongdan and Xie, Jun}, doi = {10.1016/j.biortech.2018.02.008}, journal-iso = {BIORESOUR TECHNOL}, journal = {BIORESOURCE TECHNOLOGY}, volume = {257}, unique-id = {27600332}, issn = {0960-8524}, year = {2018}, eissn = {1873-2976}, pages = {23-29} } @article{MTMT:27570764, title = {Production of Bio-Ethanol by Integrating Microwave-Assisted Dilute Sulfuric Acid Pretreated Sugarcane Bagasse Slurry with Molasses}, url = {https://m2.mtmt.hu/api/publication/27570764}, author = {Yu, Na and Tan, Li and Sun, Zhao-Yong and Tang, Yue-Qin and Kida, Kenji}, doi = {10.1007/s12010-017-2651-9}, journal-iso = {APPL BIOCHEM BIOTECH}, journal = {APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY}, volume = {185}, unique-id = {27570764}, issn = {0273-2289}, year = {2018}, eissn = {1559-0291}, pages = {191-206} } @article{MTMT:26752398, title = {Production of electricity from ethanol and ammonium based wastewater via photo-electrochemical process}, url = {https://m2.mtmt.hu/api/publication/26752398}, author = {Ibrahim, N and Kamarudin, S K and Minggu, L J}, doi = {10.1016/j.ijhydene.2016.04.137}, journal-iso = {INT J HYDROGEN ENERG}, journal = {INTERNATIONAL JOURNAL OF HYDROGEN ENERGY}, volume = {42}, unique-id = {26752398}, issn = {0360-3199}, year = {2017}, eissn = {1879-3487}, pages = {9051-9062} } @article{MTMT:26921451, title = {Prospecting Agro-waste Cocktail: Supplementation for Cellulase Production by a Newly Isolated Thermophilic B-licheniformis 2D55}, url = {https://m2.mtmt.hu/api/publication/26921451}, author = {Kazeem, Muinat Olanike and Shah, Umi Kalsom Md and Baharuddin, Azhari Samsu and AbdulRahman, Nor' Aini}, doi = {10.1007/s12010-017-2401-z}, journal-iso = {APPL BIOCHEM BIOTECH}, journal = {APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY}, volume = {182}, unique-id = {26921451}, issn = {0273-2289}, year = {2017}, eissn = {1559-0291}, pages = {1318-1340} } 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journal-iso = {GREEN CHEM}, journal = {GREEN CHEMISTRY}, volume = {19}, unique-id = {26921450}, issn = {1463-9262}, year = {2017}, eissn = {1463-9270}, pages = {3468-3492} } @article{MTMT:26752397, title = {Integrating starchy substrate into cellulosic ethanol production to boost ethanol titers and yields}, url = {https://m2.mtmt.hu/api/publication/26752397}, author = {Xu, Youjie and Wang, Donghai}, doi = {10.1016/j.apenergy.2017.03.035}, journal-iso = {APPL ENERG}, journal = {APPLIED ENERGY}, volume = {195}, unique-id = {26752397}, issn = {0306-2619}, year = {2017}, eissn = {1872-9118}, pages = {196-203} } @article{MTMT:26228765, title = {Recent trends in bioethanol production from food processing byproducts}, url = {https://m2.mtmt.hu/api/publication/26228765}, author = {Akbas, Meltem Yesilcimen and Stark, Benjamin C}, doi = {10.1007/s10295-016-1821-z}, journal-iso = {J IND MICROBIOL BIOT}, journal = {JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY}, volume = {43}, unique-id = {26228765}, issn = {1367-5435}, year = {2016}, eissn = {1476-5535}, pages = {1593-1609} } @article{MTMT:25428772, title = {Harvest index and straw yield of five classes of wheat}, url = {https://m2.mtmt.hu/api/publication/25428772}, author = {Dai, Jing and Bean, Brent and Brown, Bradford and Bruening, William and Edwards, Jeff and Flowers, Michael and Karow, Russell and Lee, Chad and Morgan, Gaylon and Ottman, Michael and Ransom, Joel and Wiersma, Jochum}, doi = {10.1016/j.biombioe.2015.12.023}, journal-iso = {BIOMASS BIOENERGY}, journal = {BIOMASS & BIOENERGY}, volume = {85}, unique-id = {25428772}, issn = {0961-9534}, year = {2016}, eissn = {1873-2909}, pages = {223-227} } @article{MTMT:26041116, title = {Ethanol production from a biomass mixture of furfural residues with green liquor-peroxide saccarified cassava liquid}, url = {https://m2.mtmt.hu/api/publication/26041116}, author = {Ji, Li and Zheng, Tianran and Zhao, Pengxiang and Zhang, Weiming and Jiang, Jianxin}, doi = {10.1186/s12896-016-0278-5}, journal-iso = {BMC BIOTECHNOL}, journal = {BMC BIOTECHNOLOGY}, volume = {16}, unique-id = {26041116}, issn = {1472-6750}, year = {2016}, eissn = {1472-6750} } @article{MTMT:25799133, title = {Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila}, url = {https://m2.mtmt.hu/api/publication/25799133}, author = {Ji, Shi-Qi and Wang, Bing and Lu, Ming and Li, Fu-Li}, doi = {10.1186/s13068-016-0494-1}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {9}, unique-id = {25799133}, issn = {1754-6834}, year = {2016}, eissn = {1754-6834} } @article{MTMT:25360257, title = {Techno-economic evaluation of integrated first- and second-generation ethanol production from grain and straw}, url = {https://m2.mtmt.hu/api/publication/25360257}, author = {Joelsson, Elisabeth and Erdei, Borbala and Galbe, Mats and Wallberg, Ola}, doi = {10.1186/s13068-015-0423-8}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {9}, unique-id = {25360257}, issn = {1754-6834}, year = {2016}, eissn = {1754-6834} } @article{MTMT:26395020, title = {Reuse of concentrated cellulose wastewater: Microwave-assisted synthesis of organic fertilizer with water-retaining property}, url = {https://m2.mtmt.hu/api/publication/26395020}, author = {Ma, Wei and Duan, Shibo and Tan, Dazhi and Cheng, Zihong and Meng, Fanqing and Yang, Lei and Li, Xuechao}, doi = {10.1016/j.jece.2015.06.019}, journal-iso = {J ENVIRON CHEM ENG}, journal = {JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING}, volume = {4}, unique-id = {26395020}, issn = {2213-3437}, year = {2016}, eissn = {2213-3437}, pages = {511-515} } @article{MTMT:26395021, title = {Using tomography images to study the mixing of wheat straw slurries}, url = {https://m2.mtmt.hu/api/publication/26395021}, author = {Movafagh, Hiva and Turcotte, Ginette and Ein-Mozaffari, Farhad}, doi = {10.1080/17597269.2015.1138038}, journal-iso = {BIOFUELS-UK}, journal = {BIOFUELS}, volume = {7}, unique-id = {26395021}, issn = {1759-7269}, year = {2016}, eissn = {1759-7277}, pages = {365-375} } @article{MTMT:26395019, title = {Enhanced Endoglucanase Production by Bacillus aerius on Mixed Lignocellulosic Substrates}, url = {https://m2.mtmt.hu/api/publication/26395019}, author = {Oke, Mushafau Adebayo and Annuar, Mohamad Suffian Mohamad and Simarani, Khanom}, doi = {10.15376/biores.11.3.5854-5869}, journal-iso = {BIORESOURCES}, journal = {BIORESOURCES}, volume = {11}, unique-id = {26395019}, issn = {1930-2126}, year = {2016}, eissn = {1930-2126}, pages = {5854-5869} } @article{MTMT:26228767, title = {Optimization of Enzymatic Hydrolysis of Steam Pretreated Triticale Straw}, url = {https://m2.mtmt.hu/api/publication/26228767}, author = {Pengilly, C and Garcia-Aparicio, M P and Diedericks, D and Gorgens, J F}, doi = {10.1007/s12155-016-9741-3}, journal-iso = {BIOENERG RES}, journal = {BIOENERGY RESEARCH}, volume = {9}, unique-id = {26228767}, issn = {1939-1234}, year = {2016}, eissn = {1939-1242}, pages = {851-863} } @article{MTMT:26041117, title = {Yield-determining components in high-solid integrated first and second generation bioethanol production from cassava residues, furfual residues and corn}, url = {https://m2.mtmt.hu/api/publication/26041117}, author = {Tang, Yong and Dou, Xiaoli and Jiang, Jianxin and Lei, Fuhou and Liu, Zuguang}, doi = {10.1039/c6ra08036g}, journal-iso = {RSC ADV}, journal = {RSC ADVANCES}, volume = {6}, unique-id = {26041117}, issn = {2046-2069}, year = {2016}, eissn = {2046-2069}, pages = {50373-50383} } @article{MTMT:26228766, title = {The Effect of Lignin Content and Freeness of Pulp on the Bioethanol Productivity of Jabon Wood}, url = {https://m2.mtmt.hu/api/publication/26228766}, author = {Wistara, Nyoman J and Pelawi, Rospita and Fatriasari, Widya}, doi = {10.1007/s12649-016-9510-8}, journal-iso = {WASTE BIOMASS VALORI}, journal = {WASTE AND BIOMASS VALORIZATION}, volume = {7}, unique-id = {26228766}, issn = {1877-2641}, year = {2016}, eissn = {1877-265X}, pages = {1141-1146} } @article{MTMT:25799132, title = {Elevated atmospheric [CO2] stimulates sugar accumulation and cellulose degradation rates of rice straw}, url = {https://m2.mtmt.hu/api/publication/25799132}, author = {Zhu, Chunwu and Xu, Xi and Wang, Dan and Zhu, Jianguo and Liu, Gang and Seneweera, Saman}, doi = {10.1111/gcbb.12277}, journal-iso = {GCB BIOENERGY}, journal = {GLOBAL CHANGE BIOLOGY-BIOENERGY}, volume = {8}, unique-id = {25799132}, issn = {1757-1693}, year = {2016}, eissn = {1757-1707}, pages = {579-587} } @article{MTMT:24904270, title = {Does size matter? Separations on guard columns for fast sample analysis applied to bioenergy research}, url = {https://m2.mtmt.hu/api/publication/24904270}, author = {Bauer, Stefan and Ibanez, Ana B}, doi = {10.1186/s12896-015-0159-3}, journal-iso = {BMC BIOTECHNOL}, journal = {BMC BIOTECHNOLOGY}, volume = {15}, unique-id = {24904270}, issn = {1472-6750}, year = {2015}, eissn = {1472-6750} } @article{MTMT:24901221, title = {Optimizing Ethanol and Methane Production from Steam-pretreated, Phosphoric Acid-impregnated Corn Stover}, url = {https://m2.mtmt.hu/api/publication/24901221}, author = {Bondesson, Pia-Maria and Dupuy, Aurelie and Galbe, Mats and Zacchi, Guido}, doi = {10.1007/s12010-014-1358-4}, journal-iso = {APPL BIOCHEM BIOTECH}, journal = {APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY}, volume = {175}, unique-id = {24901221}, issn = {0273-2289}, year = {2015}, eissn = {1559-0291}, pages = {1371-1388} } @article{MTMT:25360261, title = {Bioethanol production from steam explosion pretreated and alkali extracted Cistus ladanifer (rockrose)}, url = {https://m2.mtmt.hu/api/publication/25360261}, author = {Ferro, Miguel D and Fernandes, Maria C and Paulino, Ana F C and Prozil, Sonia O and Gravitis, Janis and Evtuguin, Dmitry V and Xavier, Ana M R B}, doi = {10.1016/j.bej.2015.04.009}, journal-iso = {BIOCHEM ENG J}, journal = {BIOCHEMICAL ENGINEERING JOURNAL}, volume = {104}, unique-id = {25360261}, issn = {1369-703X}, year = {2015}, eissn = {1873-295X}, pages = {98-105} } @article{MTMT:25360259, title = {Enhanced short chain fatty acids production from waste activated sludge conditioning with typical agricultural residues: carbon source composition regulates community functions}, url = {https://m2.mtmt.hu/api/publication/25360259}, author = {Guo, Zechong and Zhou, Aijuan and Yang, Chunxue and Liang, Bin and Sangeetha, Thangavel and He, Zhangwei and Wang, Ling and Cai, Weiwei and Wang, Aijie and Liu, Wenzong}, doi = {10.1186/s13068-015-0369-x}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {8}, unique-id = {25360259}, issn = {1754-6834}, year = {2015}, eissn = {1754-6834} } @article{MTMT:24901526, title = {Sustainable bio-ethanol production from agro-residues: A review}, url = {https://m2.mtmt.hu/api/publication/24901526}, author = {Gupta, Anubhuti and Verma, Jay Prakash}, doi = {10.1016/j.rser.2014.08.032}, journal-iso = {RENEW SUST ENERG REV}, journal = {RENEWABLE & SUSTAINABLE ENERGY REVIEWS}, volume = {41}, unique-id = {24901526}, issn = {1364-0321}, year = {2015}, eissn = {1879-0690}, pages = {550-567} } @article{MTMT:24904269, title = {Enhanced Ethanol Production with Mixed Lignocellulosic Substrates from Commercial Furfural and Cassava Residues}, url = {https://m2.mtmt.hu/api/publication/24904269}, author = {Ji, Li and Yu, Hailong and Liu, Zhiping and Jiang, Jianxin and Sun, Dafeng}, journal-iso = {BIORESOURCES}, journal = {BIORESOURCES}, volume = {10}, unique-id = {24904269}, issn = {1930-2126}, year = {2015}, eissn = {1930-2126}, pages = {1162-1173} } @article{MTMT:25635413, title = {Xylose fermentation as a challenge for commercialization of lignocellulosic fuels and chemicals}, url = {https://m2.mtmt.hu/api/publication/25635413}, author = {Nogue, Violeta Sanchez and Karhumaa, Kaisa}, doi = {10.1007/s10529-014-1756-2}, journal-iso = {BIOTECHNOL LETT}, journal = {BIOTECHNOLOGY LETTERS}, volume = {37}, unique-id = {25635413}, issn = {0141-5492}, year = {2015}, eissn = {1573-6776}, pages = {761-772} } @article{MTMT:24904268, title = {From wheat straw to bioethanol: integrative analysis of a separate hydrolysis and co-fermentation process with implemented enzyme production}, url = {https://m2.mtmt.hu/api/publication/24904268}, author = {Novy, Vera and Longus, Karin and Nidetzky, Bernd}, doi = {10.1186/s13068-015-0232-0}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {8}, unique-id = {24904268}, issn = {1754-6834}, year = {2015}, eissn = {1754-6834} } @article{MTMT:25360262, title = {Analysis of Wheat Straw Biodiversity for Use as a Feedstock for Biofuel Production}, url = {https://m2.mtmt.hu/api/publication/25360262}, author = {Tishler, Yifat and Samach, Aviva and Rogachev, Ilana and Elbaum, Rivka and Levy, Avraham A}, doi = {10.1007/s12155-015-9631-0}, journal-iso = {BIOENERG RES}, journal = {BIOENERGY RESEARCH}, volume = {8}, unique-id = {25360262}, issn = {1939-1234}, year = {2015}, eissn = {1939-1242}, pages = {1831-1839} } @article{MTMT:25360323, title = {Fermentation of rice bran hydrolysate to ethanol using Zymomonas mobilis biofilm immobilization on DEAE-cellulose}, url = {https://m2.mtmt.hu/api/publication/25360323}, author = {Todhanakasem, Tatsaporn and Narkmit, Tipong and Areerat, Kamonchanok and Thanonkeo, Pornthap}, doi = {10.1016/j.ejbt.2015.03.007}, journal-iso = {ELECTRON J BIOTECHN}, journal = {ELECTRONIC JOURNAL OF BIOTECHNOLOGY}, volume = {18}, unique-id = {25360323}, issn = {0717-3458}, year = {2015}, eissn = {0717-3458}, pages = {196-201} } @article{MTMT:24656302, title = {Co-fermentation of hemicellulose and starch from barley straw and grain for efficient pentoses utilization in acetone-butanol-ethanol production}, url = {https://m2.mtmt.hu/api/publication/24656302}, author = {Yang, M and Kuittinen, S and Zhang, JH and Vepsalainen, J and Keinanen, M and Pappinen, A}, doi = {10.1016/j.biortech.2014.12.005}, journal-iso = {BIORESOUR TECHNOL}, journal = {BIORESOURCE TECHNOLOGY}, volume = {179}, unique-id = {24656302}, issn = {0960-8524}, year = {2015}, eissn = {1873-2976}, pages = {128-135} } @article{MTMT:24656311, title = {Influence of dilute acid and alkali pretreatment on reducing sugar production from corncobs by crude enzymatic method: A comparative study}, url = {https://m2.mtmt.hu/api/publication/24656311}, author = {Baadhe, RR and Potumarthi, R and Mekala, NK}, doi = {10.1016/j.biortech.2014.03.117}, journal-iso = {BIORESOUR TECHNOL}, journal = {BIORESOURCE TECHNOLOGY}, volume = {162}, unique-id = {24656311}, issn = {0960-8524}, year = {2014}, eissn = {1873-2976}, pages = {213-217} } @article{MTMT:24662609, title = {Do New Cellulolytic Enzyme Preparations Affect the Industrial Strategies for High Solids Lignocellulosic Ethanol Production?}, url = {https://m2.mtmt.hu/api/publication/24662609}, author = {Cannella, D and Jorgensen, H}, doi = {10.1002/bit.25098}, journal-iso = {BIOTECHNOL BIOENG}, journal = {BIOTECHNOLOGY AND BIOENGINEERING}, volume = {111}, unique-id = {24662609}, issn = {0006-3592}, year = {2014}, eissn = {1097-0290}, pages = {59-68} } @article{MTMT:24656306, title = {Life cycle greenhouse gas (GHG) impacts of a novel process for converting food waste to ethanol and co-products}, url = {https://m2.mtmt.hu/api/publication/24656306}, author = {Ebner, J and Babbitt, C and Winer, M and Hilton, B and Williamson, A}, doi = {10.1016/j.apenergy.2014.04.099}, journal-iso = {APPL ENERG}, journal = {APPLIED ENERGY}, volume = {130}, unique-id = {24656306}, issn = {0306-2619}, year = {2014}, eissn = {1872-9118}, pages = {86-93} } @article{MTMT:24656309, title = {Increased bioethanol production from commercial tobacco cultivars overexpressing thioredoxin f grown under field conditions}, url = {https://m2.mtmt.hu/api/publication/24656309}, author = {Farran, I and Fernandez-San, Millan A and Ancin, M and Larraya, L and Veramendi, J}, doi = {10.1007/s11032-014-0047-x}, journal-iso = {MOL BREEDING}, journal = {MOLECULAR BREEDING}, volume = {34}, unique-id = {24656309}, issn = {1380-3743}, year = {2014}, eissn = {1572-9788}, pages = {457-469} } @article{MTMT:24685081, title = {On-site cellulase production by Trichoderma reesei 3EMS35 mutant and same vessel saccharification and fermentation of acid treated wheat straw for ethanol production}, url = {https://m2.mtmt.hu/api/publication/24685081}, author = {Khokhar, ZU and Syed, QU and Wu, J and Athar, MA}, journal-iso = {EXCLI J}, journal = {EXCLI JOURNAL}, volume = {13}, unique-id = {24685081}, issn = {1611-2156}, year = {2014}, eissn = {1611-2156}, pages = {82-97} } @article{MTMT:24656312, title = {Characteristics of Corn Stover Pretreated with Liquid Hot Water and Fed-Batch Semi-Simultaneous Saccharification and Fermentation for Bioethanol Production}, url = {https://m2.mtmt.hu/api/publication/24656312}, author = {Li, XZ and Lu, J and Zhao, J and Qu, YB}, doi = {10.1371/journal.pone.0095455}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {9}, unique-id = {24656312}, issn = {1932-6203}, year = {2014}, eissn = {1932-6203} } @article{MTMT:24656313, title = {Process intensification through microbial strain evolution: mixed glucose-xylose fermentation in wheat straw hydrolyzates by three generations of recombinant Saccharomyces cerevisiae}, url = {https://m2.mtmt.hu/api/publication/24656313}, author = {Novy, V and Krahulec, S and Wegleiter, M and Muller, G and Longus, K and Klimacek, M and Nidetzky, B}, doi = {10.1186/1754-6834-7-49}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {7}, unique-id = {24656313}, issn = {1754-6834}, year = {2014}, eissn = {1754-6834} } @article{MTMT:24656308, title = {Biofilm production by Zymomonas mobilis enhances ethanol production and tolerance to toxic inhibitors from rice bran hydrolysate}, url = {https://m2.mtmt.hu/api/publication/24656308}, author = {Todhanakasem, T and Sangsutthiseree, A and Areerat, K and Young, GM and Thanonkeo, P}, doi = {10.1016/j.nbt.2014.06.002}, journal-iso = {NEW BIOTECHNOL}, journal = {NEW BIOTECHNOLOGY}, volume = {31}, unique-id = {24656308}, issn = {1871-6784}, year = {2014}, eissn = {1876-4347}, pages = {451-459} } @article{MTMT:24656310, title = {The use of (green field) biomass pretreatment liquor for fermentative butanol production and the catalytic oxidation of biobutanol}, url = {https://m2.mtmt.hu/api/publication/24656310}, author = {Yang, M and Kuittinen, S and Keinanen, M and Vepsalainen, J and Romar, H and Tynjala, P and Lassi, U and Pappinen, A}, doi = {10.1016/j.cherd.2014.01.015}, journal-iso = {CHEM ENG RES DES}, journal = {CHEMICAL ENGINEERING RESEARCH AND DESIGN}, volume = {92}, unique-id = {24656310}, issn = {0263-8762}, year = {2014}, eissn = {1744-3563}, pages = {1531-1538} } @article{MTMT:24003685, title = {Possibilities for sustainable biorefineries based on agricultural residues - A case study of potential straw-based ethanol production in Sweden}, url = {https://m2.mtmt.hu/api/publication/24003685}, author = {Ekman, A and Wallberg, O and Joelsson, E and Börjesson, P}, doi = {10.1016/j.apenergy.2012.07.016}, journal-iso = {APPL ENERG}, journal = {APPLIED ENERGY}, volume = {102}, unique-id = {24003685}, issn = {0306-2619}, year = {2013}, eissn = {1872-9118}, pages = {299-308} } @article{MTMT:24003690, title = {Simultaneous saccharification and co-fermentation of whole wheat in integrated ethanol production}, url = {https://m2.mtmt.hu/api/publication/24003690}, author = {Erdei, B and Galbe, M and Zacchi, G}, doi = {10.1016/j.biombioe.2013.05.032}, journal-iso = {BIOMASS BIOENERGY}, journal = {BIOMASS & BIOENERGY}, volume = {56}, unique-id = {24003690}, issn = {0961-9534}, year = {2013}, eissn = {1873-2909}, pages = {506-514} } @article{MTMT:24656317, title = {SSF of steam-pretreated wheat straw with the addition of saccharified or fermented wheat meal in integrated bioethanol production}, url = {https://m2.mtmt.hu/api/publication/24656317}, author = {Erdei, B and Hancz, D and Galbe, M and Zacchi, G}, doi = {10.1186/1754-6834-6-169}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {6}, unique-id = {24656317}, issn = {1754-6834}, year = {2013}, eissn = {1754-6834} } @{MTMT:24003689, title = {Fermentable Sugars from Lignocellulosic Biomass: Technical Challenges}, url = {https://m2.mtmt.hu/api/publication/24003689}, author = {Vijai Kumar, Gupta and Tuohy, Maria G and Potumarthi, Ravichandra and Baadhe, RamaRaju and Bhattacharya, Sankar}, booktitle = {Biofuel Technologies}, doi = {10.1007/978-3-642-34519-7_1}, publisher = {Springer Netherlands}, unique-id = {24003689}, year = {2013}, pages = {3-27} } @article{MTMT:24003691, title = {p-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans}, url = {https://m2.mtmt.hu/api/publication/24003691}, author = {Kawai, Y and Noda, S and Ogino, C and Takeshima, Y and Okai, N and Tanaka, T and Kondo, A}, doi = {10.1186/1475-2859-12-45}, journal-iso = {MICROB CELL FACT}, journal = {MICROBIAL CELL FACTORIES}, volume = {12}, unique-id = {24003691}, issn = {1475-2859}, year = {2013}, eissn = {1475-2859} } @article{MTMT:24003687, title = {Bioconversion of sawdust into ethanol using dilute sulfuric acid-assisted continuous twin screw-driven reactor pretreatment and fed-batch simultaneous saccharification and fermentation}, url = {https://m2.mtmt.hu/api/publication/24003687}, author = {Kim, T H and Choi, C H and Oh, K K}, doi = {10.1016/j.biortech.2012.11.125}, journal-iso = {BIORESOUR TECHNOL}, journal = {BIORESOURCE TECHNOLOGY}, volume = {130}, unique-id = {24003687}, issn = {0960-8524}, year = {2013}, eissn = {1873-2976}, pages = {306-313} } @article{MTMT:24003688, title = {Waste paper sludge as a potential biomass for bio-ethanol production}, url = {https://m2.mtmt.hu/api/publication/24003688}, author = {Prasetyo, J and Park, E Y}, doi = {10.1007/s11814-013-0003-1}, journal-iso = {KOREAN J CHEM ENG}, journal = {KOREAN JOURNAL OF CHEMICAL ENGINEERING}, volume = {30}, unique-id = {24003688}, issn = {0256-1115}, year = {2013}, eissn = {1975-7220}, pages = {253-261} } @article{MTMT:23983114, title = {Feasibility of simultaneous saccharification and juice co-fermentation on hydrothermal pretreated sweet sorghum bagasse for ethanol production}, url = {https://m2.mtmt.hu/api/publication/23983114}, author = {Rohowsky, B and Häßler, T and Gladis, A and Remmele, E and Schieder, D and Faulstich, M}, doi = {10.1016/j.apenergy.2012.03.039}, journal-iso = {APPL ENERG}, journal = {APPLIED ENERGY}, volume = {102}, unique-id = {23983114}, issn = {0306-2619}, year = {2013}, eissn = {1872-9118}, pages = {211-219} } @article{MTMT:24003677, title = {Saccharification versus simultaneous saccharification and fermentation of kraft pulp}, url = {https://m2.mtmt.hu/api/publication/24003677}, author = {Bauer, N A and Gibbons, W R}, journal-iso = {IJABE}, journal = {INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING}, volume = {5}, unique-id = {24003677}, issn = {1934-6344}, year = {2012}, eissn = {1934-6352}, pages = {48-55} } @article{MTMT:24003676, title = {Separate hydrolysis and co-fermentation for improved xylose utilization in integrated ethanol production from wheat meal and wheat straw}, url = {https://m2.mtmt.hu/api/publication/24003676}, author = {Borbála, Erdei and Balázs, Frankó and Mats, Galbe and Guido, Zacchi}, doi = {10.1186/1754-6834-5-12}, journal-iso = {BIOTECHNOL BIOFUELS}, journal = {BIOTECHNOLOGY FOR BIOFUELS}, volume = {5}, unique-id = {24003676}, issn = {1754-6834}, year = {2012}, eissn = {1754-6834}, pages = {1-12} } @article{MTMT:2685405, title = {Process considerations of a biorefinery producing value-added products from corn fibre}, url = {https://m2.mtmt.hu/api/publication/2685405}, author = {Fehér, Csaba and Barta, Zsolt and Réczey, Istvánné}, doi = {10.3311/pp.ch.2012-1.02}, journal-iso = {PERIOD POLYTECH CHEM ENG}, journal = {PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING}, volume = {56}, unique-id = {2685405}, issn = {0324-5853}, year = {2012}, eissn = {1587-3765}, pages = {9-19} } @article{MTMT:24003684, title = {Iron Oxide Filled Magnetic Carbon Nanotube-Enzyme Conjugates for Recycling of Amyloglucosidase: Toward Useful Applications in Biofuel Production Process}, url = {https://m2.mtmt.hu/api/publication/24003684}, author = {Goh, Wei Jiang and Makam, Venkata S and Hu, Jun and Kang, Lifeng and Zheng, Minrui and Yoong, Sia Lee and Udalagama, Chammika N B and Pastorin, Giorgia}, doi = {10.1021/la303046m}, journal-iso = {LANGMUIR}, journal = {LANGMUIR}, volume = {28}, unique-id = {24003684}, issn = {0743-7463}, year = {2012}, eissn = {1520-5827}, pages = {16864-16873} } @article{MTMT:23975422, title = {Ethanol Production from Soybean Fiber, a Co-product of Aqueous Oil Extraction, Using a Soaking in Aqueous Ammonia Pretreatment}, url = {https://m2.mtmt.hu/api/publication/23975422}, author = {Karki, B and Maurer, D and Box, S and Kim, TH and Jung, S}, doi = {10.1007/s11746-012-2016-z}, journal-iso = {J AM OIL CHEM SOC}, journal = {JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY}, volume = {89}, unique-id = {23975422}, issn = {0003-021X}, year = {2012}, eissn = {1558-9331}, pages = {1345-1353} } @article{MTMT:24003680, title = {Benzoic acid fermentation from starch and cellulose via a plant-like β-oxidation pathway in Streptomyces maritimus}, url = {https://m2.mtmt.hu/api/publication/24003680}, author = {Noda, S and Kitazono, E and Tanaka, T and Ogino, C and Kondo, A}, doi = {10.1186/1475-2859-11-49}, journal-iso = {MICROB CELL FACT}, journal = {MICROBIAL CELL FACTORIES}, volume = {11}, unique-id = {24003680}, issn = {1475-2859}, year = {2012}, eissn = {1475-2859} } @article{MTMT:24003682, title = {Comparison of Escherichia coli KO11 and Saccharomyces cerevisiae ATCC 96581 in fermenting Pinus patula hydrolysate pretreated at different steam explosion severity}, url = {https://m2.mtmt.hu/api/publication/24003682}, author = {Nyangi, Chacha and Nils, Dyrset and Godliving, Mtui and Jamidu, Katima and Godwill, Mrema}, journal-iso = {AFR J BIOTECHNOL}, journal = {AFRICAN JOURNAL OF BIOTECHNOLOGY}, volume = {11}, unique-id = {24003682}, issn = {1684-5315}, year = {2012}, pages = {9054-9063} }