TY - JOUR AU - Dixit, Mandeep AU - Shukla, Pratyoosh TI - Multi-efficient endoglucanase from Aspergillus niger MPS25 and its potential applications in saccharification of wheat straw and waste paper deinking JF - CHEMOSPHERE J2 - CHEMOSPHERE VL - 313 PY - 2023 PG - 10 SN - 0045-6535 DO - 10.1016/j.chemosphere.2022.137298 UR - https://m2.mtmt.hu/api/publication/33891586 ID - 33891586 AB - The deinking in the paper industry is in great demand, and microbial enzymes are key players. In the present study, the endoglucanase production from newly isolated fungi Aspergillus niger MPS25 is reported. The optimization of endoglucanase production was carried out using one factor at a time approach resulting in endoglucanase activity (2.37 IU/ml) at 45 degrees C and pH 8 in submerged fermentation (SmF), which shows optimum enzyme activity at 60 degrees C. Interestingly, the metal ions viz. Co2+ stimulated the endoglucanase activity, whereas Mn2+ reduced the enzyme activity, which shows that this enzyme can be used for effluent treatment released through deinking. The enzymatic hydrolysis of wheat straw produced 26.96 +/- 0.108 mg/g of reducing sugars, indicating its potential in saccharification and the biofuel industry. Furthermore, the validation of the deinking efficiency of this enzyme resulted in improved deinking of mixed office waste and old newspapers by 31.5% and 20.4%, respectively. The strength properties, viz. burst factor and tear index, breaking length, and tensile index of the handmade paper sheets, were also improved which were analyzed by the scanning electron micrographs. The FTIR and XRD analysis of pulp provided insights into the changes in functional groups and cellulose crystallinity, respectively. These results indicate that multi-efficient endoglucanase from Aspergillus niger MPS25 is suitable for enzyme-based eco-friendly deinking for waste paper recycling and lignocellulosic biomass saccharification. LA - English DB - MTMT ER - TY - JOUR AU - Novy, Vera AU - Nielsen, Fredrik AU - Seiboth, Bernhard AU - Nidetzky, Bernd TI - The influence of feedstock characteristics on enzyme production in Trichoderma reesei. a review on productivity, gene regulation and secretion profiles. TS - a review on productivity, gene regulation and secretion profiles. JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 12 PY - 2019 PG - 16 SN - 1754-6834 DO - 10.1186/s13068-019-1571-z UR - https://m2.mtmt.hu/api/publication/30879237 ID - 30879237 N1 - Journal Article; Review AB - Biorefineries, designed for the production of lignocellulose-based chemicals and fuels, are receiving increasing attention from the public, governments, and industries. A major obstacle for biorefineries to advance to commercial scale is the high cost of the enzymes required to derive the fermentable sugars from the feedstock used. As summarized in this review, techno-economic studies suggest co-localization and integration of enzyme manufacturing with the cellulosic biorefinery as the most promising alternative to alleviate this problem. Thus, cultivation of Trichoderma reesei, the principal producer of lignocellulolytic enzymes, on the lignocellulosic biomass processed on-site can reduce the cost of enzyme manufacturing. Further, due to a complex gene regulation machinery, the fungus can adjust the gene expression of the lignocellulolytic enzymes towards the characteristics of the feedstock, increasing the hydrolytic efficiency of the produced enzyme cocktail. Despite extensive research over decades, the underlying regulatory mechanisms are not fully elucidated. One aspect that has received relatively little attention in literature is the influence the characteristics of a lignocellulosic substrate, i.e., its chemical and physical composition, has on the produced enzyme mixture. Considering that the fungus is dependent on efficient enzymatic degradation of the lignocellulose for continuous supply of carbon and energy, a relationship between feedstock characteristics and secretome composition can be expected. The aim of this review was to systematically collect, appraise, and aggregate data and integrate results from studies analyzing enzyme production by T. reesei on insoluble cellulosic model substrates and lignocellulosic biomass. The results show that there is a direct effect of the substrate's complexity (rated by structure, composition of the lignin-carbohydrate complex, and recalcitrance in enzymatic saccharification) on enzyme titers and the composition of specific activities in the secretome. It further shows that process-related factors, such as substrate loading and cultivation set-up, are direct targets for increasing enzyme yields. The literature on transcriptome and secretome composition further supports the proposed influence of substrate-related factors on the expression of lignocellulolytic enzymes. This review provides insights into the interrelation between the characteristics of the substrate and the enzyme production by T. reesei, which may help to advance integrated enzyme manufacturing of substrate-specific enzymes cocktails at scale. LA - English DB - MTMT ER - TY - JOUR AU - Bagewadi, Zabin K AU - Mulla, Sikandar I AU - Ninnekar, Harichandra Z TI - Optimization of endoglucanase production from Trichoderma harzianum strain HZN11 by central composite design under response surface methodology JF - BIOMASS CONVERSION AND BIOREFINERY J2 - BIOMASS CONV BIOREFINERY VL - 8 PY - 2018 IS - 2 SP - 305 EP - 316 PG - 12 SN - 2190-6815 DO - 10.1007/s13399-017-0285-3 UR - https://m2.mtmt.hu/api/publication/27567136 ID - 27567136 LA - English DB - MTMT ER - TY - JOUR AU - de Eugenio, L I AU - Mendez-Liter, J A AU - de los, Rios V AU - Prieto, A AU - Martinez, M J TI - beta-1,4-endoglucanases from Talaromyces amestolkiae: Production of glucooligosaccharides from different beta-glucans JF - BIOCATALYSIS AND BIOTRANSFORMATION J2 - BIOCATAL BIOTRANSFOR VL - 36 PY - 2018 IS - 1 SP - 68 EP - 77 PG - 10 SN - 1024-2422 DO - 10.1080/10242422.2017.1306741 UR - https://m2.mtmt.hu/api/publication/27314653 ID - 27314653 LA - English DB - MTMT ER - TY - JOUR AU - de Eugenio, Laura I AU - Mendez-Liter, Juan A AU - Nieto-Dominguez, Manuel AU - Alonso, Lola AU - Gil-Munoz, Jesus AU - Barriuso, Jorge AU - Prieto, Alicia AU - Jesus, Martinez Maria TI - Differential beta-glucosidase expression as a function of carbon source availability in Talaromyces amestolkiae: a genomic and proteomic approach JF - BIOTECHNOLOGY FOR BIOFUELS J2 - BIOTECHNOL BIOFUELS VL - 10 PY - 2017 PG - 14 SN - 1754-6834 DO - 10.1186/s13068-017-0844-7 UR - https://m2.mtmt.hu/api/publication/26750346 ID - 26750346 LA - English DB - MTMT ER - TY - JOUR AU - Zheng, Wei AU - Zheng, Qin AU - Xue, Yiyun AU - Hu, Jiajun AU - Gao, Min-Tian TI - Influence of rice straw polyphenols on cellulase production by Trichoderma reesei JF - JOURNAL OF BIOSCIENCE AND BIOENGINEERING J2 - J BIOSCI BIOENG VL - 123 PY - 2017 IS - 6 SP - 731 EP - 738 PG - 8 SN - 1389-1723 DO - 10.1016/j.jbiosc.2017.01.009 UR - https://m2.mtmt.hu/api/publication/26749415 ID - 26749415 LA - English DB - MTMT ER - 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167 PY - 2012 IS - 5 SP - 959 EP - 972 PG - 14 SN - 0273-2289 DO - 10.1007/s12010-012-9645-4 UR - https://m2.mtmt.hu/api/publication/23975336 ID - 23975336 LA - English DB - MTMT ER - TY - JOUR AU - Rodriguez-Gomez, D AU - Lehmann, L AU - Schultz-Jensen, N AU - Bjerre, AB AU - Hobley, TJ TI - Examining the Potential of Plasma-Assisted Pretreated Wheat Straw for Enzyme Production by Trichoderma reesei JF - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY J2 - APPL BIOCHEM BIOTECH VL - 166 PY - 2012 IS - 8 SP - 2051 EP - 2063 PG - 13 SN - 0273-2289 DO - 10.1007/s12010-012-9631-x UR - https://m2.mtmt.hu/api/publication/24033023 ID - 24033023 LA - English DB - MTMT ER - TY - JOUR AU - de Castro, AM AU - de Carvalho, MLD AU - Leite, SGF AU - Pereira, N TI - Cellulases from Penicillium funiculosum: production, properties and application to cellulose hydrolysis JF - JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY J2 - J IND MICROBIOL BIOT VL - 37 PY - 2010 IS - 2 SP - 151 EP - 158 PG - 8 SN - 1367-5435 DO - 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23975332 LA - English DB - MTMT ER - TY - JOUR AU - Ike, M AU - Park, J AU - Tabuse, M AU - Tokuyasu, K TI - Cellulase production on glucose-based media by the UV-irradiated mutants of Trichoderma reesei JF - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY J2 - APPL MICROBIOL BIOT VL - 87 PY - 2010 IS - 6 SP - 2059 EP - 2066 PG - 8 SN - 0175-7598 DO - 10.1007/s00253-010-2683-3 UR - https://m2.mtmt.hu/api/publication/24030530 ID - 24030530 LA - English DB - MTMT ER - TY - JOUR AU - Kumar, R AU - Singh, S AU - Singh, OV TI - Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives JF - JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY J2 - J IND MICROBIOL BIOT VL - 35 PY - 2008 IS - 5 SP - 377 EP - 391 PG - 15 SN - 1367-5435 DO - 10.1007/s10295-008-0327-8 UR - https://m2.mtmt.hu/api/publication/23975341 ID - 23975341 LA - English DB - MTMT ER - TY - JOUR AU - Elfstrand, S AU - Hedlund, K AU - Martensson, A TI - Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring JF - APPLIED SOIL ECOLOGY J2 - APPL SOIL ECOL VL - 35 PY - 2007 IS - 3 SP - 610 EP - 621 PG - 12 SN - 0929-1393 DO - 10.1016/j.apsoil.2006.09.011 UR - https://m2.mtmt.hu/api/publication/23975330 ID - 23975330 LA - English DB - MTMT ER -