TY - JOUR AU - Palacon, Mariana Paravani AU - Barbeiro, Camila de Oliveira AU - Fernandes, Darcy AU - Biancardi, Mariel Ruivo AU - Silveira, Heitor Albergoni AU - Ferrisse, Tulio Morandin AU - Leon, Jorge Esquiche AU - Kujan, Omar AU - Bufalino, Andreia TI - Macrophages CD163+and Factor XIIIa plus Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 6 PG - 12 SN - 1661-6596 DO - 10.3390/ijms24065242 UR - https://m2.mtmt.hu/api/publication/33887202 ID - 33887202 AB - This study aimed to evaluate the density of the dendritic cells (DCs) and macrophages in oral leukoplakia (OL) and proliferative verrucous leukoplakia (PVL) by immunohistochemical analysis. We analysed paraffined tissue samples of PVL (n = 27), OL (n = 20), and inflammatory fibrous hyperplasia (n = 20) as the control group using the immunomarkers for DCs (CD1a, CD207, CD83, CD208 and CD123) and macrophages (CD68, CD163, FXIIIa and CD209). A quantitative analysis of positive cells in the epithelial and subepithelial areas was determined. Our results showed a reduction in CD208+ cells in the subepithelial area of the OL and PVL compared to the control. Additionally, we found a higher density of FXIIIa+ and CD163+ cells in the subepithelial area in PVL compared to the OL and control. Four-way MANOVA revealed a relationship between increased CD123+ cell density in the subepithelial area of "high-risk" samples regardless of disease. Macrophages provide the first line of defence against PVL antigens, suggesting a distinct pattern of innate immune system activation in PVL compared to OL, which may contribute to the complexity and the high rate of malignant transformation in the PVL. LA - English DB - MTMT ER - TY - JOUR AU - Dull, Katalin AU - Fazekas, Fruzsina AU - Törőcsik, Dániel TI - Factor XIII-A in Diseases: Role Beyond Blood Coagulation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 3 PG - 14 SN - 1661-6596 DO - 10.3390/ijms22031459 UR - https://m2.mtmt.hu/api/publication/31848446 ID - 31848446 LA - English DB - MTMT ER - TY - JOUR AU - Nakai, K. TI - Multiple roles of macrophage in skin JF - JOURNAL OF DERMATOLOGICAL SCIENCE J2 - J DERMATOL SCI VL - 104 PY - 2021 IS - 1 SP - 2 EP - 10 PG - 9 SN - 0923-1811 DO - 10.1016/j.jdermsci.2021.08.008 UR - https://m2.mtmt.hu/api/publication/32894638 ID - 32894638 AB - More than 100 years have passed since Elie Metchnikoff discovered macrophage. Over the recent decade, attracting information about macrophage polarization have been reported. This is because many molecules have been identified as markers of macrophage polarization. Additionally, mechanistic insights have been demonstrated by experiments with various stimuli-induced macrophage polarization. Historically and simply, macrophages are divided into M1 (classically activated) and M2 (alternatively activated). However, some of them are not specific yet. Studies in the field of cardiology revealed the plasticity of macrophages and their subsets are divided into details: Mhem, MHb, Mox and M4 macrophages. M2 macrophages were further divided in M2a, M2b, M2c and M2d. There appears to be more phenotypes of macrophages. However, there still lack studies in dermatological field. This review summarizes the spectrum of macrophage activation and finding about various roles of macrophages in the dermatological field. © 2021 LA - English DB - MTMT ER - TY - JOUR AU - Buergler, Christina AU - Vinay, Keshavamurthy AU - Haefliger, Stefanie AU - Kloetgen, Hans-Wilhelm AU - Yawalkar, Nikhil TI - Infliximab reduces activated myeloid dendritic cells, different macrophage subsets and CXCR3-positive cells in granuloma annulare JF - JOURNAL OF DERMATOLOGY J2 - J DERMATOL VL - 46 PY - 2019 IS - 9 SP - 808 EP - 811 PG - 4 SN - 0385-2407 DO - 10.1111/1346-8138.14981 UR - https://m2.mtmt.hu/api/publication/30967582 ID - 30967582 AB - Disseminated granuloma annulare (GA) is a rare granulomatous dermatitis of unknown etiology. Treatment is often challenging and lack of a uniformly effective treatment, adds to the disease morbidity. Tumor necrosis factor (TNF)-alpha is an important cytokine in granuloma formation and previous reports have shown improvement of disseminated GA with anti-TNF-alpha therapy. Nevertheless, the underlying mechanism of actions of TNF-alpha inhibitors in GA remains unclear. Our aim was to evaluate alterations in the inflammatory infiltrate in a patient who experienced complete clearance of GA after treatment with infliximab. A skin biopsy was obtained before and 24 weeks after treatment with infliximab 5 mg/kg at weeks 0, 2, 6, 14 and 24. Immunohistochemical stains were performed in pre- and post-treatment biopsy specimens using CD1a, CD4, CD8, CD11c, CD32, CD68, CD69, CD163, CD183 and human leukocyte antigen (HLA)-DR to characterize alterations of the infiltrates. Parallel with clinical improvement, we observed a marked decrease in myeloid (CD11c) dendritic cells, different macrophage subsets (CD68, CD32, CD163) and T cells. In addition, a marked reduction of activation markers (HLA-DR, CD69) and CD183(+) (CXCR3) cells was observed in post-treatment biopsy specimens. In conclusion, the clinical improvement of disseminated GA by infliximab is paralleled by inhibition of activated myeloid dendritic cells, different macrophage subsets and type 1 T cells. LA - English DB - MTMT ER - TY - JOUR AU - Pap, Nives AU - Bradamante, Mirna AU - Hadzavdic, Suzana Ljubojevic TI - Localized Perforating Granuloma Annulare JF - ACTA DERMATOVENEROLOGICA CROATICA J2 - ACTA DERMATOVENER CR VL - 27 PY - 2019 IS - 1 SP - 33 EP - 36 PG - 4 SN - 1330-027X UR - https://m2.mtmt.hu/api/publication/30967583 ID - 30967583 AB - Perforating granuloma annulare (PGA) is a rare type of granulomatous skin disease. The etiology and pathogenesis of PGA are still unknown. Diagnosis and treatment of PGA is complex and challenging. We present the case of a 31-year-old male patient with a localized form of PGA successfully treated with topical steroids. LA - English DB - MTMT ER - TY - JOUR AU - Lugo-Villarino, Geanncarlo AU - Troegeler, Anthony AU - Balboa, Luciana AU - Lastrucci, Claire AU - Duval, Carine AU - Mercier, Ingrid AU - Benard, Alan AU - Capilla, Florence AU - Al, Saati Talal AU - Poincloux, Renaud AU - Kondova, Ivanela AU - Verreck, Frank A W AU - Cougoule, Celine AU - Maridonneau-Parini, Isabelle AU - Sasiain, Maria del Carmen AU - Neyrolles, Olivier TI - The C-Type Lectin Receptor DC-SIGN Has an Anti-Inflammatory Role in Human M(IL-4) Macrophages in Response to Mycobacterium tuberculosis JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 9 PY - 2018 PG - 15 SN - 1664-3224 DO - 10.3389/fimmu.2018.01123 UR - https://m2.mtmt.hu/api/publication/27613525 ID - 27613525 LA - English DB - MTMT ER - TY - JOUR AU - Asai, Jun TI - What is new in the histogenesis of granulomatous skin diseases? JF - JOURNAL OF DERMATOLOGY J2 - J DERMATOL VL - 44 PY - 2017 IS - 3 SP - 297 EP - 303 PG - 7 SN - 0385-2407 DO - 10.1111/1346-8138.13662 UR - https://m2.mtmt.hu/api/publication/26562207 ID - 26562207 LA - English DB - MTMT ER - TY - JOUR AU - Lovászi, Marianna AU - Mattii, M AU - Eyerich, K AU - Gácsi, Attila AU - Csányi, Erzsébet AU - Kovács, Dóra AU - Ruhl, R AU - Szegedi, Andrea AU - Kemény, Lajos AU - Stahle, M AU - Zouboulis, CC AU - Eyerich, S AU - Törőcsik, Dániel TI - Sebum lipids influence macrophage polarization and activation. JF - BRITISH JOURNAL OF DERMATOLOGY J2 - BRIT J DERMATOL VL - 177 PY - 2017 IS - 6 SP - 1671 EP - 1682 PG - 12 SN - 0007-0963 DO - 10.1111/bjd.15754 UR - https://m2.mtmt.hu/api/publication/3244185 ID - 3244185 AB - BACKGROUND: As lipids are known to regulate macrophage functions it is reasonable to suppose that a sebocyte - macrophage axis mediated by sebum lipids may exist. OBJECTIVE: To investigate if sebocytes could contribute to the differentiation, polarization and function of macrophages with their secreted lipids. METHODS: Oil-red-O lipid staining and Raman spectroscopy were used to assess the dermal lipid content and penetration. Immunohistochemistry was used to analyse the macrophage subsets. Human peripheral blood monocytes were differentiated in the presence of either supernatant from human SZ95 sebocytes or major sebum lipid components and activated with Propionibacterium acnes. Macrophage surface markers and their capacity to uptake FITC-Propionibacterium acnes were detected by FACS measurements. Cytokine protein levels were evaluated by ELISA and Western blot analysis. RESULTS: Sebaceous gland rich skin had an increased dermal lipid content compared to sebaceous gland poor skin to which all the tested sebum component lipids could contribute by penetrating through the dermo-epidermal barrier. Of the lipids, oleic and linoleic acids promoted monocyte differentiation into alternatively activated macrophages. Moreover, linoleic acid also had an anti-inflammatory effect in Propionibacterium acnes activated macrophages, inhibiting the secretion of IL-1B, IL-6 and TNF-Alpha. Squalene, palmitic, stearic and oleic acids augmented the secretion of IL-1B even in the absence of Propionibacterium acnes, while oleic acid had a selective effect of inducing IL-1B, but down-regulating IL-6 and TNF-A secretion. CONCLUSIONS: Our results suggest a role for sebaceous glands in modulating innate immune responses via their secreted lipids that are of possible pathologic and therapeutic relevance. This article is protected by copyright. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Paragh, L AU - Törőcsik, Dániel TI - Factor XIII Subunit A in the Skin: Applications in Diagnosis and Treatment JF - BIOMED RESEARCH INTERNATIONAL J2 - BIOMED RES INT VL - 2017 PY - 2017 PG - 14 SN - 2314-6133 DO - 10.1155/2017/3571861 UR - https://m2.mtmt.hu/api/publication/3345044 ID - 3345044 N1 - Megjegyzés-26828059 N1 Funding details: ERDF, European Regional Development Fund N1 Funding text: Daniel Törócsik was supported through the New National Excellence Program of the Ministry of Human Capacities and by the Hungarian National Research Fund (OTKA NN117020). The work/publication is supported by the GINOP-2.3.2-15-2016-00005 Project. The project is cofinanced by the European Union and the European Regional Development Fund. AB - The role of factor XIII subunit A (FXIII-A) is not restricted to hemostasis. FXIII-A is also present intracellularly in several human cells and serves as a diagnostic marker in a wide range of dermatological diseases from inflammatory conditions to malignancies. In this review, we provide a guide on the still controversial interpretation of dermal cell types expressing FXIII-A and assess the previously described mechanisms behind their accumulation under physiological and pathological conditions of the human skin. We summarize the intracellular functions of FXIII-A as well as its possible sources in the extracellular space of the dermis with a focus on its relevance to skin homeostasis and disease pathogenesis. Finally, the potential role of FXIII-A in wound healing, as a field with long-term therapeutic implications, is also discussed. LA - English DB - MTMT ER - TY - JOUR AU - Vroman, H AU - Hendriks, RW AU - Kool, M TI - Dendritic cell subsets in asthma: Impaired Tolerance or exaggerated inflammation? JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 8 PY - 2017 SN - 1664-3224 DO - 10.3389/fimmu.2017.00941 UR - https://m2.mtmt.hu/api/publication/26824715 ID - 26824715 LA - English DB - MTMT ER - TY - JOUR AU - Soendergaard, Christoffer AU - Kvist, Peter Helding AU - Seidelin, Jakob Benedict AU - Pelzer, Hermann AU - Nielsen, Ole Haagen TI - Systemic and intestinal levels of factor XIII-A: the impact of inflammation on expression in macrophage subtypes JF - JOURNAL OF GASTROENTEROLOGY J2 - J GASTROENTEROL VL - 51 PY - 2016 IS - 8 SP - 796 EP - 807 PG - 12 SN - 0944-1174 DO - 10.1007/s00535-015-1152-2 UR - https://m2.mtmt.hu/api/publication/26215576 ID - 26215576 LA - English DB - MTMT ER - TY - JOUR AU - Alves, J. AU - Barreiros, H. AU - Bártolo, E. TI - Perforating granuloma annulare — an unusual subtype of a common disease JF - HEALTHCARE J2 - HEALTHCARE-BASEL VL - 2 PY - 2014 IS - 3 SP - 338 EP - 345 PG - 8 SN - 2227-9032 DO - 10.3390/healthcare2030338 UR - https://m2.mtmt.hu/api/publication/32894640 ID - 32894640 AB - Perforating granuloma annulare (GA) is a rare subset of GA with an unknown etiology and chronic course. Herein, we report the case of 72 year-old women with a 3-month history of a post-traumatic, persistent, erythematous and exudative plaque located on her left leg. Differential diagnosis included mycobacterial infection, subcutaneous mycosis, perforating dermatoses, pyoderma and squamous cell carcinoma. The histopathology was highly suggestive of a perforating GA. The patient was treated with betamethasone dipropionate cream applied once daily and a complete resolution of the lesion was observed in three weeks. Despite being a very rare subtype of a common disease, perforating granuloma annulare has clinical and histopathological characteristic features that facilitate the differential diagnosis, avoiding unnecessary procedures and inadequate and potentially more invasive treatments. © 2014 by the authors; licensee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Silvano, A TI - Dendritic cells: Phenotypic and functional heterogeneity JF - ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY = ARCHIVIO ITALIANO DI ANATOMIA J2 - ITAL J ANAT EMBRYOL VL - 119 PY - 2014 IS - 3 SP - 304 EP - 330 PG - 27 SN - 1122-6714 DO - 10.13128/IJAE-15563 UR - https://m2.mtmt.hu/api/publication/24590645 ID - 24590645 LA - English DB - MTMT ER -