@article{MTMT:34446740, title = {Distinctive features of Zaprionus indianus hemocyte differentiation and function revealed by transcriptomic analysis}, url = {https://m2.mtmt.hu/api/publication/34446740}, author = {Cinege, Gyöngyi Ilona and Magyar, Lilla Brigitta and Kovács, Henrietta and Varga, Viktória and Bodai, László and Zsindely, Nóra and Nagy, Gábor and Hegedűs, Zoltán and Hultmark, Dan and Andó, István}, doi = {10.3389/fimmu.2023.1322381}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {14}, unique-id = {34446740}, issn = {1664-3224}, year = {2023}, eissn = {1664-3224}, orcid-numbers = {Bodai, László/0000-0001-8411-626X; Zsindely, Nóra/0000-0002-6189-3100; Nagy, Gábor/0000-0001-5464-1135; Andó, István/0000-0002-4648-9396} } @article{MTMT:34576395, title = {The human leukemic oncogene MLL-AF4 promotes hyperplastic growth of hematopoietic tissues in Drosophila larvae}, url = {https://m2.mtmt.hu/api/publication/34576395}, author = {Johannessen, J.A. and Formica, M. and Haukeland, A.L.C. and Bråthen, N.R. and Al, Outa A. and Aarsund, M. and Therrien, M. and Enserink, J.M. and Knævelsrud, H.}, doi = {10.1016/j.isci.2023.107726}, journal-iso = {ISCIENCE}, journal = {ISCIENCE}, volume = {26}, unique-id = {34576395}, abstract = {MLL-rearranged (MLL-r) leukemias are among the leukemic subtypes with poorest survival, and treatment options have barely improved over the last decades. Despite increasing molecular understanding of the mechanisms behind these hematopoietic malignancies, this knowledge has had poor translation into the clinic. Here, we report a Drosophila melanogaster model system to explore the pathways affected in MLL-r leukemia. We show that expression of the human leukemic oncogene MLL-AF4 in the Drosophila hematopoietic system resulted in increased levels of circulating hemocytes and an enlargement of the larval hematopoietic organ, the lymph gland. Strikingly, depletion of Drosophila orthologs of known interactors of MLL-AF4, such as DOT1L, rescued the leukemic phenotype. In agreement, treatment with small-molecule inhibitors of DOT1L also prevented the MLL-AF4-induced leukemia-like phenotype. Taken together, this model provides an in vivo system to unravel the genetic interactors involved in leukemogenesis and offers a system for improved biological understanding of MLL-r leukemia. © 2023 The Author(s)}, keywords = {Oncology; Molecular Biology; CELL BIOLOGY}, year = {2023}, eissn = {2589-0042} } @article{MTMT:33555087, title = {A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/33555087}, author = {Kúthy-Sutus, Enikő and Kharrat, Bayan and Gábor, Erika and Csordás, Gábor and Sinka, Rita and Honti, Viktor}, doi = {10.3390/cells12010024}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {12}, unique-id = {33555087}, abstract = {The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo.}, year = {2023}, eissn = {2073-4409}, orcid-numbers = {Kúthy-Sutus, Enikő/0000-0002-1398-4120; Csordás, Gábor/0000-0001-6871-6839; Sinka, Rita/0000-0003-4040-4184} } @article{MTMT:34261480, title = {Insect-pathogen crosstalk and the cellular-molecular mechanisms of insect immunity: uncovering the underlying signaling pathways and immune regulatory function of non-coding RNAs}, url = {https://m2.mtmt.hu/api/publication/34261480}, author = {Mahanta, Deepak Kumar and Bhoi, Tanmaya Kumar and Komal, J. and Samal, Ipsita and Nikhil, R. M. and Paschapur, Amit Umesh and Singh, Gaurav and Kumar, P. V. Dinesh and Desai, H. R. and Ahmad, Mohammad Abbas and Singh, P. P. and Majhi, Prasanta Kumar and Mukherjee, U. and Singh, Pushpa and Saini, Varun and Shahanaz, N. and Srinivasa, N. and Yele, Yogesh}, doi = {10.3389/fimmu.2023.1169152}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {14}, unique-id = {34261480}, issn = {1664-3224}, abstract = {Multicellular organisms are constantly subjected to pathogens that might be harmful. Although insects lack an adaptive immune system, they possess highly effective anti-infective mechanisms. Bacterial phagocytosis and parasite encapsulation are some forms of cellular responses. Insects often defend themselves against infections through a humoral response. This phenomenon includes the secretion of antimicrobial peptides into the hemolymph. Specific receptors for detecting infection are required for the recognition of foreign pathogens such as the proteins that recognize glucans and peptidoglycans, together referred to as PGRPs and & beta;GRPs. Activation of these receptors leads to the stimulation of signaling pathways which further activates the genes encoding for antimicrobial peptides. Some instances of such pathways are the JAK-STAT, Imd, and Toll. The host immune response that frequently accompanies infections has, however, been circumvented by diseases, which may have assisted insects evolve their own complicated immune systems. The role of ncRNAs in insect immunology has been discussed in several notable studies and reviews. This paper examines the most recent research on the immune regulatory function of ncRNAs during insect-pathogen crosstalk, including insect- and pathogen-encoded miRNAs and lncRNAs, and provides an overview of the important insect signaling pathways and effector mechanisms activated by diverse pathogen invaders.}, keywords = {INSECT; immune system; SIGNALING PATHWAY; Antimicrobial peptide; insect-pathogen crosstalk; effector mechanism}, year = {2023}, eissn = {1664-3224} } @article{MTMT:33777226, title = {Parasitic nematode secreted phospholipase A2 suppresses cellular and humoral immunity by targeting hemocytes in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/33777226}, author = {Parks, S.C. and Okakpu, O.K. and Azizpor, P. and Nguyen, S. and Martinez-Beltran, S. and Claudio, I. and Anesko, K. and Bhatia, A. and Dhillon, H.S. and Dillman, A.R.}, doi = {10.3389/fimmu.2023.1122451}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {14}, unique-id = {33777226}, issn = {1664-3224}, abstract = {A key aspect of parasitic nematode infection is the nematodes’ ability to evade and/or suppress host immunity. This immunomodulatory ability is likely driven by the release of hundreds of excretory/secretory proteins (ESPs) during infection. While ESPs have been shown to display immunosuppressive effects on various hosts, our understanding of the molecular interactions between individual proteins released and host immunity requires further study. We have recently identified a secreted phospholipase A2 (sPLA2) released from the entomopathogenic nematode (EPN) Steinernema carpocapsae we have named Sc-sPLA2. We report that Sc-sPLA2 increased mortality of Drosophila melanogaster infected with Streptococcus pneumoniae and promoted increased bacterial growth. Furthermore, our data showed that Sc-sPLA2 was able to downregulate both Toll and Imd pathway-associated antimicrobial peptides (AMPs) including drosomycin and defensin, in addition to suppressing phagocytosis in the hemolymph. Sc-sPLA2 was also found to be toxic to D. melanogaster with the severity being both dose- and time-dependent. Collectively, our data highlighted that Sc-sPLA2 possessed both toxic and immunosuppressive capabilities. Copyright © 2023 Parks, Okakpu, Azizpor, Nguyen, Martinez-Beltran, Claudio, Anesko, Bhatia, Dhillon and Dillman.}, keywords = {Animals; Male; PHAGOCYTOSIS; PHENOTYPE; ARTICLE; IMMUNOMODULATION; MORTALITY; DROSOPHILA; DROSOPHILA; HEMOCYTES; animal; polymerase chain reaction; high performance liquid chromatography; physiology; amino acid sequence; immunosuppressive agent; Escherichia coli; defensin; nonhuman; animal experiment; Host-Parasite Interactions; Host-Parasite Interactions; Immunity; microbiology; disease severity; Nematoda; host parasite interaction; nematode; parasite; in vitro study; enzyme activity; Mass spectrometry; Gene Expression; Drosophila melanogaster; Drosophila melanogaster; Drosophila melanogaster; protein expression; Arachidonic Acid; phosphatidylcholine; cell culture; phospholipase a2; humoral immunity; humoral immunity; cellular immunity; blood cell; blood cell; protein synthesis; protein purification; metabolomics; bacterial growth; real time polymerase chain reaction; Streptococcus pneumoniae; LIPIDOMICS; Liquid chromatography-Mass spectrometry; electrospray; sPLA2; colony forming unit; phosphoserine; anion exchange; Hemolymph; polypeptide antibiotic agent; ultra performance liquid chromatography; Immunity, Humoral; Multiple reaction monitoring; Listeria monocytogenes; monophenol monooxygenase; Phospholipases A2, Secretory; entomopathogenic nematode; Immune modulation; Steinernema carpocapsae; Secretory protein; secretory phospholipase A2; cytolysis assay; nematodiasis; phosphoethanolamine; drosomycin}, year = {2023}, eissn = {1664-3224} } @article{MTMT:34270655, title = {The Drosophila melanogaster prophenoloxidase system participates in immunity against Zika virus infection}, url = {https://m2.mtmt.hu/api/publication/34270655}, author = {Tafesh-Edwards, Ghada and Eleftherianos, Ioannis}, doi = {10.1002/eji.202350632}, journal-iso = {EUR J IMMUNOL}, journal = {EUROPEAN JOURNAL OF IMMUNOLOGY}, unique-id = {34270655}, issn = {0014-2980}, abstract = {Drosophila melanogaster relies on an evolutionarily conserved innate immune system to protect itself from a wide range of pathogens, making it a convenient genetic model to study various human pathogenic viruses and host antiviral immunity. Here we explore for the first time the contribution of the Drosophila phenoloxidase (PO) system to host survival and defenses against Zika virus (ZIKV) infection by analyzing the role of mutations in the three prophenoloxidase (PPO) genes in female and male flies. We show that only PPO1 and PPO2 genes contribute to host survival and appear to be upregulated following ZIKV infection in Drosophila. Also, we present data suggesting that a complex regulatory system exists between Drosophila PPOs, potentially allowing for a sex-dependent compensation of PPOs by one another or other immune responses such as the Toll, Imd, and JAK/STAT pathways. Furthermore, we show that PPO1 and PPO2 are essential for melanization in the hemolymph and the wound site in flies upon ZIKV infection. Our results reveal an important role played by the melanization pathway in response to ZIKV infection, hence highlighting the importance of this pathway in insect host defense against viral pathogens and potential vector control strategies to alleviate ZIKV outbreaks.}, keywords = {Drosophila melanogaster; Zika virus; Antiviral immunity; prophenoloxidase}, year = {2023}, eissn = {1521-4141}, orcid-numbers = {Eleftherianos, Ioannis/0000-0002-4822-3110} } @article{MTMT:33288601, title = {Drosophila melanogaster as a model to study innate immune memory}, url = {https://m2.mtmt.hu/api/publication/33288601}, author = {Arch, M. and Vidal, M. and Koiffman, R. and Melkie, S.T. and Cardona, P.-J.}, doi = {10.3389/fmicb.2022.991678}, journal-iso = {FRONT MICROBIOL}, journal = {FRONTIERS IN MICROBIOLOGY}, volume = {13}, unique-id = {33288601}, issn = {1664-302X}, abstract = {Over the last decades, research regarding innate immune responses has gained increasing importance. A growing body of evidence supports the notion that the innate arm of the immune system could show memory traits. Such traits are thought to be conserved throughout evolution and provide a survival advantage. Several models are available to study these mechanisms. Among them, we find the fruit fly, Drosophila melanogaster. This non-mammalian model has been widely used for innate immune research since it naturally lacks an adaptive response. Here, we aim to review the latest advances in the study of the memory mechanisms of the innate immune response using this animal model. Copyright © 2022 Arch, Vidal, Koiffman, Melkie and Cardona.}, keywords = {RESISTANCE; MEMORY; INFECTION; TOLERANCE; review; innate immunity; nonhuman; animal model; animal experiment; Drosophila melanogaster; Drosophila melanogaster; immunological memory; Innate immune memory; trained immunity}, year = {2022}, eissn = {1664-302X} } @{MTMT:33288603, title = {Insects and nanoparticles}, url = {https://m2.mtmt.hu/api/publication/33288603}, author = {Bhattacherje, R. and Ghosh, S. and Banerjee, D.}, booktitle = {Nanoparticles and the Immune System: Volume 2: Immune System of Animals}, doi = {10.1515/9783110655872-004}, unique-id = {33288603}, abstract = {The insects have been studied for having a robust innate immune system and recently reported for properties of specificity and memory-like response. Different pathways that contribute to the insect immunity include TOLL, immune deficiency, mitogen-activated protein kinase, c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38 kinase pathways. Other pathways including autophagy and RNA interference pathways have been implicated in antiviral immune responses. Furthermore, different types of antimicrobial peptides also reported in insects that function in diverse ways to combat pathogenic infections. Beneficial insects are directly or indirectly involved in providing various ecosystem services, whereas harmful groups of insects are responsible for crop and stored product damage as well as transmit several types of pathogenic organisms. The induction of cellular and humoral immune responses in insects against diverse array of microbes has been studied with deeper insights with the assistance of various types of nano-particles. Nanoparticles for insect control and against insect vectors have shown promising results and can be bioengineered in different ways for getting beneficial outputs; thus, they are worth exploring. © 2022 Walter de Gruyter GmbH, Berlin/Boston. All rights reserved.}, keywords = {APOPTOSIS; INSECTS; NANOPARTICLES; Arthropoda}, year = {2022}, pages = {61-87} } @article{MTMT:32601795, title = {Effects of ammonia nitrogen stress on the blood cell immunity and liver antioxidant function of Sepia pharaonis}, url = {https://m2.mtmt.hu/api/publication/32601795}, author = {Chai, Y. and Peng, R. and Jiang, M. and Jiang, X. and Han, Q. and Han, Z.}, doi = {10.1016/j.aquaculture.2021.737417}, journal-iso = {AQUACULTURE}, journal = {AQUACULTURE}, volume = {546}, unique-id = {32601795}, issn = {0044-8486}, abstract = {The objective of this study was to investigate toxicity effects of ammonia nitrogen on the cuttlefish Sepia pharaonis. The effects of ammonia nitrogen stress (1, 3, 6, and 12 mg/L) on the blood cell immunity and liver antioxidant function were studied. The results showed that the number of blood cells, respiratory burst activities of blood cells, the phagocytosis rate of blood cells, Cu/Zn–superoxide dismutase (Cu/Zn–SOD) and catalase (CAT) activities and relative gene expression, and MDA content of the cuttlefish S. pharaonis were significantly affected by the ammonia nitrogen (P < 0.05). The number of apoptotic blood cells, respiratory burst activities of blood cells and contents of MDA were increase, phagocytosis of blood cells, SOD and CAT activity were decrease with long-term exposure to low concentration (1 mg/L) or high concentration (>3 mg/L) of ammonia nitrogen stress, and action degree is positively related to the stress of ammonia nitrogen concentration and time length treated. In summary, the ammonia nitrogen stress could result in declining phagocytic percentage, decreasing the activity of phagocytic, SOD and CAT, increasing the contents of MDA. It resulting in an imbalance of non-specific immune system and damaging antioxidant defense system, reducing the ability of eliminating free radicals, damaging cell membrane structure, then its functions of the cells and tissues of the body, which was a contributory cause of ammonia poisoning. © 2021 Elsevier B.V.}, keywords = {GENE; ENZYME; MEMBRANE; AMMONIA; ANTIOXIDANT; fish; TOXICITY; enzyme activity; immune system; Gene Expression; immunosuppression; OXIDATIVE DAMAGE; Sepia pharaonis; Sepia pharaonis; Ammonia toxicity; Sepiidae}, year = {2022}, eissn = {1873-5622} } @article{MTMT:32524824, title = {Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids}, url = {https://m2.mtmt.hu/api/publication/32524824}, author = {Cinege, Gyöngyi Ilona and Magyar, Lilla Brigitta and Kovács, Attila Lajos and Lerner, Zita and Juhász, Gábor and Lukacsovich, David and Winterer, Jochen and Lukacsovich, Tamás and Hegedűs, Zoltán and Kurucz, Judit Éva and Hultmark, Dan and Földy, Csaba and Andó, István}, doi = {10.1159/000520110}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {14}, unique-id = {32524824}, issn = {1662-811X}, year = {2022}, eissn = {1662-8128}, pages = {335-354}, orcid-numbers = {Juhász, Gábor/0000-0001-8548-8874; Winterer, Jochen/0000-0002-6800-6594; Lukacsovich, Tamás/0000-0001-5908-9861; Hultmark, Dan/0000-0002-6506-5855; Andó, István/0000-0002-4648-9396} } @article{MTMT:32365190, title = {Mechanisms and biological impacts of graphene and multi-walled carbon nanotubes on Drosophila melanogaster: Oxidative stress, genotoxic damage, phenotypic variations, locomotor behavior, parasitoid resistance, and cellular immune response}, url = {https://m2.mtmt.hu/api/publication/32365190}, author = {Demir, Esref}, doi = {10.1002/jat.4232}, journal-iso = {J APPL TOXICOL}, journal = {JOURNAL OF APPLIED TOXICOLOGY}, volume = {42}, unique-id = {32365190}, issn = {0260-437X}, abstract = {The use of graphene and multi-walled carbon nanotubes (MWCNTs) has now become rather common in medical applications as well as several other areas thanks to their useful physicochemical properties. While in vitro testing offers some potential, in vivo research into toxic effects of graphene and MWCNTs could yield much more reliable data. Drosophila melanogaster has recently gained significant popularity as a dynamic eukaryotic model in examining toxicity, genotoxicity, and biological effects of exposure to nanomaterials, including oxidative stress, cellular immune response against two strains (NSRef and G486) of parasitoid wasp (Leptopilina boulardi), phenotypic variations, and locomotor behavior risks. D. melanogaster was used as a model organism in our study to identify the potential risks of exposure to graphene (thickness: 2-18 nm) and MWCNTs in different properties (as pure [OD: 10-20 nm short], modified by amide [NH2] [OD: 7-13 nm length: 55 mu m], and modified by carboxyl [COOH] [OD: 30-50 nm and length: 0.5-2 mu m]) at concentrations ranging from 0.1 to 250 mu g/ml. Significant effects were observed at two high doses (100 and 250 mu g/ml) of graphene or MWCNTs. This is the first study to report findings of cellular immune response against hematopoiesis and parasitoids, nanogenotoxicity, phenotypic variations, and locomotor behavior in D. melanogaster.}, keywords = {Drosophila melanogaster; Graphene; genotoxicity; Leptopilina boulardi; Phenotypic variations; Cellular immune response; Multi walled carbon nanotubes; Locomotor behavior; drosophila parasitoids}, year = {2022}, eissn = {1099-1263}, pages = {450-474} } @article{MTMT:33178825, title = {Irradiation and parasitism affect the ability of larval hemocytes of Anastrepha obliqua for phagocytosis and the production of reactive oxygen species}, url = {https://m2.mtmt.hu/api/publication/33178825}, author = {Gomez-Alonso, Itzia and Baltierra-Uribe, Shantal and Sanchez-Torres, Luvia and Cancino-Diaz, Mario and Cancino-Diaz, Juan and Rodriguez-Martinez, Sandra and Ovruski, Sergio M. and Hendrichs, Jorge and Cancino, Jorge}, doi = {10.1002/arch.21953}, journal-iso = {ARCH INSECT BIOCHEM}, journal = {ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY}, unique-id = {33178825}, issn = {0739-4462}, abstract = {The development of the parasitoid Doryctobracon crawfordi (Viereck) (Hymenoptera: Braconidae) in Anastrepha obliqua (McQuart) (Diptera: Tephritidae) larvae is unviable in nature; however, if the host larva is irradiated at 160 Gy, the parasitoid develops and emerges successfully. This suggests that radiation affects the immune responses of A. obliqua larvae, while the underlying mechanisms remain to be revealed. Using optical and electronic microscopies we determined the number and type of hemocyte populations found inside the A. obliqua larvae, either nonirradiated, irradiated at 160 Gy, parasitized by D. crawfordi, or irradiated and parasitized. Based on flow cytometry, the capacity to produce reactive oxygen species (ROS) was determined by the 123-dihydrorhodamine method in those hemocyte cells. Five cell populations were found in the hemolymph of A. obliqua larvae, two of which (granulocytes and plasmatocytes) can phagocytize and produce ROS. A reduction in the number of cells, mainly of the phagocytic type, was observed, as well as the capacity of these cells to produce ROS, when A. obliqua larvae were irradiated. Both radiation and parasitization decreased the ROS production, and when A. obliqua larvae were irradiated followed by parasitization by D. crawfordi, the reduction of the ROS level was even greater. In contrast, a slight increase in the size of these cells was observed in the hemolymph of the parasitized larvae compared to those in nonparasitized larvae. These results suggest that radiation significantly affects the phagocytic cells of A. obliqua and thus permits the development of the parasitoid D. crawfordi.}, keywords = {PHAGOCYTOSIS; Flow Cytometry; Reactive oxygen species; immune responses; host radiation}, year = {2022}, eissn = {1520-6327} } @article{MTMT:33039275, title = {Hematopoietic plasticity mapped in Drosophila and other insects}, url = {https://m2.mtmt.hu/api/publication/33039275}, author = {Hultmark, Dan and Andó, István}, doi = {10.7554/eLife.78906}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {11}, unique-id = {33039275}, issn = {2050-084X}, year = {2022}, eissn = {2050-084X}, orcid-numbers = {Hultmark, Dan/0000-0002-6506-5855; Andó, István/0000-0002-4648-9396} } @article{MTMT:32876730, title = {IDENTIFICATION OF A NEW CRUSTIN AND STUDY OF ITS ANTIBACTERIAL FUNCTION FROM PROCAMBARUS CLARKII}, url = {https://m2.mtmt.hu/api/publication/32876730}, author = {Jiao, H.-Q. and Huo, S.-T. and Yan, L.-M. and Li, Y.-H. and Liu, X.-Q.}, doi = {10.7541/2021.2019.280}, journal-iso = {ACTA HYDROBIOLOGICA SINICA}, journal = {ACTA HYDROBIOLOGICA SINICA}, volume = {46}, unique-id = {32876730}, issn = {1000-3207}, abstract = {The crayfish (Procambarus clarkii) is one of the important economic shrimp species which is widely distributed in the southeastern and central regions of China. In recent years, with the continous rise of peoples’ demand and the continous development of aquaculture industry, various diseases in the aquaculture have frequently outbroken, including bacterial and viral diseases, causing huge economic losses to the crayfish culture industry. As an invertebrate Procambarus clarkii lacks an adaptive immune system, which mainly resists the invasion of pathogens through the innate immune system. Antimicrobial peptide (AMP), as an important effector in the invertebrate’s innate immune system, is one of the substitutes for traditional antibiotics due to its broad-spectrum antibacterial activity and resistance to drug resistance. Crustin is one of the most widely studied antibacterial peptide in Procambarus clarikii. This study amplified and identified a new type of Crustin from Procambarus clarikii, named Pc-CruL. In order to reveal the function of Pc-CruL in Procambarus clarikii, antibacterial activity was determined by means of recombinant expression in vitro. It was hoped to reveal its relationship with pathogens in the form of the prevention and control of the disease and the opening of medicines for Procambarus clarikii. Amplifying the Pc-CruL gene in Procambarus clarikii and ligating this gene to the PGEX-4T-1 vector to construct a prokaryotic expression plasmid. The recombinant plasmid was induced and purified in competent BL21 (DE3) for in vitro and in vivo antibacterial experiment. The CDS region of Pc-CruL contains 330 bp and encodes 109 amino acids. Pc-CruL is expressed in all tissues of normal Procambarus clarikii, with higher expression levels in hemocytes and relatively lower expression levels in hepatopancreas. The results of liquid antibacterial assay showed that Pc-CruL protein significantly restricted the growth of Vibrio parahaemolyticus, Staphylococcus aureus, Aeromonas hydrophila, and Aeromonas veronii, and had broad-spectrum antibacterial activity. The injection of exogenous recombinant Pc-CruL protein could improve the survival rate of infection with Vibrio parahaemolyticus. In general, Pc-CruL is an important immune molecule in Procambarus clarikii and plays a vital role in defending against bacterial infections. © 2022, Institute of Hydrobiology, Chinese Academy of Sciences. All rights reserved.}, keywords = {innate immunity; ANTIMICROBIAL PEPTIDES; Vibrio parahaemolyticus; Procambarus clarkii; crustin}, year = {2022}, pages = {248-256} } @article{MTMT:33050458, title = {Peeling Back the Layers of Lymph Gland Structure and Regulation}, url = {https://m2.mtmt.hu/api/publication/33050458}, author = {Kharrat, Bayan and Csordás, Gábor and Honti, Viktor}, doi = {10.3390/ijms23147767}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {23}, unique-id = {33050458}, issn = {1661-6596}, abstract = {During the past 60 years, the fruit fly, Drosophila melanogaster, has proven to be an excellent model to study the regulation of hematopoiesis. This is not only due to the evolutionarily conserved signalling pathways and transcription factors contributing to blood cell fate, but also to convergent evolution that led to functional similarities in distinct species. An example of convergence is the compartmentalization of blood cells, which ensures the quiescence of hematopoietic stem cells and allows for the rapid reaction of the immune system upon challenges. The lymph gland, a widely studied hematopoietic organ of the Drosophila larva, represents a microenvironment with similar features and functions to classical hematopoietic stem cell niches of vertebrates. Lymph gland studies were effectively supported by the unparalleled toolkit developed in Drosophila, which enabled the high-resolution investigation of the cellular composition and regulatory interaction networks of the lymph gland. In this review, we summarize how our understanding of lymph gland structure and hematopoietic cell-to-cell communication evolved during the past decades and compare their analogous features to those of the vertebrate hematopoietic stem cell niche.}, keywords = {IMMUNE-RESPONSE; DROSOPHILA; matrix protein; Hematopoiesis; SELF-RENEWAL; HEMATOPOIETIC STEM-CELL; Biochemistry & Molecular Biology; HSC; N-cadherin; lymph gland; EMBRYONIC ORIGIN; Drosophila larvae; PROGENITOR MAINTENANCE; HEMOCYTE LINEAGES}, year = {2022}, eissn = {1422-0067}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839} } @article{MTMT:32876726, title = {Intrinsic and Extrinsic Regulation of Hematopoiesis in Drosophila}, url = {https://m2.mtmt.hu/api/publication/32876726}, author = {Koranteng, F. and Cho, B. and Shim, J.}, doi = {10.14348/molcells.2022.2039}, journal-iso = {MOL CELLS}, journal = {MOLECULES AND CELLS}, volume = {45}, unique-id = {32876726}, issn = {1016-8478}, abstract = {Drosophila melanogaster lymph gland, the primary site of hematopoiesis, contains myeloid-like progenitor cells that differentiate into functional hemocytes in the circulation of pupae and adults. Fly hemocytes are dynamic and plastic, and they play diverse roles in the innate immune response and wound healing. Various hematopoietic regulators in the lymph gland ensure the developmental and functional balance between progenitors and mature blood cells. In addition, systemic factors, such as nutrient availability and sensory inputs, integrate environmental variabilities to synchronize the blood development in the lymph gland with larval growth, physiology, and immunity. This review examines the intrinsic and extrinsic factors determining the progenitor states during hemocyte development in the lymph gland and provides new insights for further studies that may extend the frontier of our collective knowledge on hematopoiesis and innate immunity. © 2022, Korean Society for Molecular and Cellular Biology. All rights reserved.}, keywords = {Animals; Adult; PEPTIDE; calcium; DROSOPHILA; DROSOPHILA; HEMOCYTES; stem cell; human; animal; short survey; physiology; innate immunity; nonhuman; larva; larva; sensory stimulation; Wound healing; Drosophila melanogaster; Drosophila melanogaster; Drosophila melanogaster; human cell; adenosine; blood cell; reactive oxygen metabolite; hematopoietic stem cell; 4 aminobutyric acid; HYPOXIA; beta Catenin; malnutrition; platelet derived growth factor; lymph node; Hematopoiesis; Hematopoiesis; Hematopoiesis; succinic acid; Hematopoietic Stem Cells; Notch receptor; uvomorulin; Drosophila Proteins; nutrient availability; Drosophila protein; Pupa; tricarboxylic acid; calcium calmodulin dependent protein kinase II; forkhead transcription factor; frizzled protein; infestation; protein Patched; lymph gland; Drosophila hematopoiesis; hemocyte differentiation; inter-organ regulation; niche regulation; progenitor cell maintenance}, year = {2022}, eissn = {0219-1032}, pages = {101-108} } @article{MTMT:32876724, title = {Advances in the Immune Regulatory Role of Non-Coding RNAs (miRNAs and lncRNAs) in Insect-Pathogen Interactions}, url = {https://m2.mtmt.hu/api/publication/32876724}, author = {Moure, U.A.E. and Tan, T. and Sha, L. and Lu, X. and Shao, Z. and Yang, G. and Wang, Y. and Cui, H.}, doi = {10.3389/fimmu.2022.856457}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {13}, unique-id = {32876724}, issn = {1664-3224}, abstract = {Insects are by far the most abundant and diverse living organisms on earth and are frequently prone to microbial attacks. In other to counteract and overcome microbial invasions, insects have in an evolutionary way conserved and developed immune defense mechanisms such as Toll, immune deficiency (Imd), and JAK/STAT signaling pathways leading to the expression of antimicrobial peptides. These pathways have accessory immune effector mechanisms, such as phagocytosis, encapsulation, melanization, nodulation, RNA interference (RNAi), lysis, autophagy, and apoptosis. However, pathogens evolved strategies that circumvent host immune response following infections, which may have helped insects further sophisticate their immune response mechanisms. The involvement of ncRNAs in insect immunity is undeniable, and several excellent studies or reviews have investigated and described their roles in various insects. However, the functional analyses of ncRNAs in insects upon pathogen attacks are not exhaustive as novel ncRNAs are being increasingly discovered in those organisms. This article gives an overview of the main insect signaling pathways and effector mechanisms activated by pathogen invaders and summarizes the latest findings of the immune modulation role of both insect- and pathogen-encoded ncRNAs, especially miRNAs and lncRNAs during insect–pathogen crosstalk. Copyright © 2022 Moure, Tan, Sha, Lu, Shao, Yang, Wang and Cui.}, keywords = {Animals; GENETICS; INSECT; animal; RNA Interference; RNA Interference; insecta; microRNA; MICRORNAS; RNA, Untranslated; untranslated RNA; long untranslated RNA; Immune modulation; RNA, Long Noncoding; insect immune pathways; insect–pathogen interaction; miRNAs and lncRNAs; mRNA targets}, year = {2022}, eissn = {1664-3224} } @article{MTMT:32876727, title = {Review of the Role of Parasitic Nematode Excretory/Secretory Proteins in Host Immunomodulation}, url = {https://m2.mtmt.hu/api/publication/32876727}, author = {Okakpu, O.K. and Dillman, A.R.}, doi = {10.1645/21-33}, journal-iso = {J PARASITOL}, journal = {JOURNAL OF PARASITOLOGY}, volume = {108}, unique-id = {32876727}, issn = {0022-3395}, abstract = {Parasitic nematodes infect a variety of organisms including insects and vertebrates. To survive, they evade host immune responses to cause morbidity and mortality. Despite the vast clinical knowledge regarding nematode infections and their biological makeup, molecular understanding of the interactions between host and parasite remains poorly understood. The utilization of model systems has thus been employed to help elucidate the molecular interactions of the host immune response during parasitic nematode infection. Using model systems, it has been well established that parasitic nematodes evade host immunity by releasing excretory/secretory proteins (ESPs), which are involved in immunomodulation. Model systems have enabled researchers to characterize further the underlying mechanisms ESPs use to facilitate evasion and modulation of the host immune response. This review assessed notable ESPs from parasitic nematodes that infect vertebrates or insects and have been studied in mechanistic detail. Being able to characterize how ESPs affect the immune systems of hosts on a molecular level increases our understanding of host-parasite interactions and could lead to the identification of novel therapeutic targets and important molecular pathways. © American Society of Parasitologists 2022.}, keywords = {Animals; PROTEIN; CELL; IMMUNOMODULATION; IMMUNOMODULATION; MORTALITY; animal; physiology; Host-Parasite Interactions; Immunity; Immunity; Nematoda; host parasite interaction; host-parasite interaction; nematode; nematode; parasite; parasitology; immune response; IL-10; morbidity; PARASITES; literature review; anti-inflammatory; B cell; T cell; MODEL SYSTEM; Heterorhabditis bacteriophora; immunomodulatory; PPO; Steinernema carpocapsae; AMPs; DCs; EPNs; nematodiasis; PO; ESPs; Nematode Infections}, year = {2022}, eissn = {1937-2345}, pages = {199-208} } @article{MTMT:33217847, title = {Gradual specialization of phagocytic ameboid cells may have impaired regenerative capacities in metazoan lineages}, url = {https://m2.mtmt.hu/api/publication/33217847}, author = {Ribeiro de Lima, Felipe Matheus and Abrahao, Isabella and Pentagna, Nathalia and Carneiro, Katia}, doi = {10.1002/dvdy.543}, journal-iso = {DEV DYNAM}, journal = {DEVELOPMENTAL DYNAMICS}, unique-id = {33217847}, issn = {1058-8388}, abstract = {Animal regeneration is a fascinating field of research that has captured the attention of many generations of scientists. Among the cellular mechanisms underlying tissue and organ regeneration, we highlight the role of phagocytic ameboid cells (PACs). Beyond their ability to engulf nutritional particles, microbes, and apoptotic cells, their involvement in regeneration has been widely documented. It has been extensively described that, at least in part, animal regenerative mechanisms rely on PACs that serve as a hub for a range of critical physiological functions, both in health and disease. Considering the phylogenetics of PAC evolution, and the loss and gain of nutritional, immunological, and regenerative potential across Metazoa, we aim to discuss when and how phagocytic activity was first co-opted to regenerative tissue repair. We propose that the gradual specialization of PACs during metazoan derivation may have contributed to the loss of regenerative potential in animals, with critical impacts on potential translational strategies for regenerative medicine.}, keywords = {phagocytic ameboid cells; animal regeneration; Metazoan derivation}, year = {2022}, eissn = {1097-0177}, orcid-numbers = {Carneiro, Katia/0000-0001-5915-5486} } @article{MTMT:33587927, title = {Hemocytes and fat body cells, the only professional immune cell types in Drosophila, show strikingly different responses to systemic infections}, url = {https://m2.mtmt.hu/api/publication/33587927}, author = {Vaibhvi, V. and Künzel, S. and Roeder, T.}, doi = {10.3389/fimmu.2022.1040510}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {13}, unique-id = {33587927}, issn = {1664-3224}, abstract = {The fruit fly Drosophila is an excellent model to study the response of different immunocompetent organs during systemic infection. In the present study, we intended to test the hypothesis that the only professional immune organs of the fly, the fat body and hemocytes, show substantial similarities in their responses to systemic infection. However, comprehensive transcriptome analysis of isolated organs revealed highly divergent transcript signatures, with the few commonly regulated genes encoding mainly classical immune effectors from the antimicrobial peptide family. The fat body and the hemocytes each have specific reactions that are not present in the other organ. Fat body-specific responses comprised those enabling an improved peptide synthesis and export. This reaction is accompanied by transcriptomic shifts enabling the use of the energy resources of the fat body more efficiently. Hemocytes, on the other hand, showed enhanced signatures related to phagocytosis. Comparing immune-induced signatures of both cell types with those of whole-body responses showed only a minimal correspondence, mostly restricted again to antimicrobial peptide genes. In summary, the two major immunocompetent cell types of Drosophila show highly specific responses to infection, which are closely linked to the primary function of the respective organ in the landscape of the systemic immune response. Copyright © 2022 Vaibhvi, Künzel and Roeder.}, keywords = {Animals; Female; Humans; ADIPOCYTES; PHAGOCYTOSIS; INFECTION; ARTICLE; signal transduction; DROSOPHILA; DROSOPHILA; HEMOCYTES; human; animal; Cell Differentiation; Escherichia coli; innate immunity; innate immunity; controlled study; nonhuman; Gene Amplification; Gene Expression; lipid metabolism; quality control; GENE ONTOLOGY; Sepsis; Sepsis; unclassified drug; messenger rna; immune response; blood cell; protein synthesis; MORPHOGENESIS; bacterial infection; Serratia marcescens; PEPTIDE SYNTHESIS; ANTIMICROBIAL PEPTIDES; transcriptomics; metabolomics; upregulation; interleukin 1beta; down regulation; Bacillus subtilis; real time polymerase chain reaction; Fluorescence microscopy; Adipose Tissue; Adipose Tissue; adipocyte; genetic transcription; RNA extraction; Transcriptome; Transcriptome; Housekeeping gene; colony forming unit; RNA sequence; Body fat; Pectobacterium carotovorum; Hemolymph; oxidative phosphorylation; polypeptide antibiotic agent; fat body; fat body; cytokine response; glycogen metabolism; FAT PAD; insect immunity; Sterile injury; adipokinetic hormone; papilin; lipid body; lipophorin; Drosophila immunity; prophenoloxidase 1; prophenoloxidase 2; fat body cell}, year = {2022}, eissn = {1664-3224} } @article{MTMT:31940120, title = {Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry}, url = {https://m2.mtmt.hu/api/publication/31940120}, author = {Balog, József Ágoston and Honti, Viktor and Kurucz, Judit Éva and Kari, Beáta and Puskás, László and Andó, István and Szebeni, Gábor}, doi = {10.1016/j.gpb.2020.06.022}, journal-iso = {GENOM PROTEOM BIOINF}, journal = {GENOMICS PROTEOMICS & BIOINFORMATICS}, volume = {19}, unique-id = {31940120}, issn = {1672-0229}, year = {2021}, eissn = {2210-3244}, pages = {243-252}, orcid-numbers = {Andó, István/0000-0002-4648-9396; Szebeni, Gábor/0000-0002-6998-5632} } @article{MTMT:32246048, title = {Deciphering of Candida parapsilosis induced immune response in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/32246048}, author = {Csonka, Katalin and Tasi, Zsolt and Vedelek, Viktor and Vágvölgyi, Csaba and Sinka, Rita and Gácser, Attila}, doi = {10.1080/21505594.2021.1980989}, journal-iso = {VIRULENCE}, journal = {VIRULENCE}, volume = {12}, unique-id = {32246048}, issn = {2150-5594}, year = {2021}, eissn = {2150-5608}, pages = {2571-2582}, orcid-numbers = {Vágvölgyi, Csaba/0000-0003-0009-7773; Sinka, Rita/0000-0003-4040-4184} } @article{MTMT:31743832, title = {There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells}, url = {https://m2.mtmt.hu/api/publication/31743832}, author = {Csordás, Gábor and Gábor, Erika and Honti, Viktor}, doi = {10.1016/j.ydbio.2020.10.006}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {469}, unique-id = {31743832}, issn = {0012-1606}, year = {2021}, eissn = {1095-564X}, pages = {135-143}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839} } @article{MTMT:32365195, title = {Identification of Silkworm Hemocyte Subsets and Analysis of Their Response to Baculovirus Infection Based on Single-Cell RNA Sequencing}, url = {https://m2.mtmt.hu/api/publication/32365195}, author = {Feng, Min and Xia, Junming and Fei, Shigang and Peng, Ruoxuan and Wang, Xiong and Zhou, Yaohong and Wang, Pengwei and Swevers, Luc and Sun, Jingchen}, doi = {10.3389/fimmu.2021.645359}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {12}, unique-id = {32365195}, issn = {1664-3224}, abstract = {A wide range of hemocyte types exist in insects but a full definition of the different subclasses is not yet established. The current knowledge of the classification of silkworm hemocytes mainly comes from morphology rather than specific markers, so our understanding of the detailed classification, hemocyte lineage and functions of silkworm hemocytes is very incomplete. Bombyx mori nucleopolyhedrovirus (BmNPV) is a representative member of the baculoviruses and a major pathogen that specifically infects silkworms (Bombyx mori) and causes serious losses in sericulture industry. Here, we performed single-cell RNA sequencing (scRNA-seq) of hemocytes in BmNPV and mock-infected larvae to comprehensively identify silkworm hemocyte subsets and determined specific molecular and cellular characteristics in each hemocyte subset before and after viral infectmadion. A total of 20 cell clusters and their potential marker genes were identified in silkworm hemocytes. All of the hemocyte clusters were infected by BmNPV at 3 days after inoculation. Interestingly, BmNPV infection can cause great changes in the distribution of hemocyte types. The cells appearing in the infection group mainly belong to prohemocytes (PR), while plasmatocytes (PL) and granulocytes (GR) are very much reduced. Furthermore, we found that BmNPV infection suppresses the RNA interference (RNAi) and immune response in the major hemocyte types. In summary, our results revealed the diversity of silkworm hemocytes and provided a rich resource of gene expression profiles for a systems-level understanding of their functions in the uninfected condition and as a response to BmNPV.}, keywords = {HEMOCYTES; Bombyx mori; BmNPV; ScRNA-seq; Lepidopteron}, year = {2021}, eissn = {1664-3224} } @article{MTMT:31867767, title = {Comparative hematopoiesis and signal transduction in model organisms}, url = {https://m2.mtmt.hu/api/publication/31867767}, author = {Gautam, D.K. and Chimata, A.V. and Gutti, R.K. and Paddibhatla, I.}, doi = {10.1002/jcp.30287}, journal-iso = {J CELL PHYSIOL}, journal = {JOURNAL OF CELLULAR PHYSIOLOGY}, volume = {236}, unique-id = {31867767}, issn = {0021-9541}, abstract = {Hematopoiesis is a continuous phenomenon involving the formation of hematopoietic stem cells (HSCs) giving rise to diverse functional blood cells. This developmental process of hematopoiesis is evolutionarily conserved, yet comparably different in various model organisms. Vertebrate HSCs give rise to all types of mature cells of both the myeloid and the lymphoid lineages sequentially colonizing in different anatomical tissues. Signal transduction in HSCs facilitates their potency and specifies branching of lineages. Understanding the hematopoietic signaling pathways is crucial to gain insights into their deregulation in several blood-related disorders. The focus of the review is on hematopoiesis corresponding to different model organisms and pivotal role of indispensable hematopoietic pathways. We summarize and discuss the fundamentals of blood formation in both invertebrate and vertebrates, examining the requirement of key signaling nexus in hematopoiesis. Knowledge obtained from such comparative studies associated with developmental dynamics of hematopoiesis is beneficial to explore the therapeutic options for hematopoietic diseases. © 2021 Wiley Periodicals LLC}, keywords = {BLOOD; MICE; ZEBRAFISH; DROSOPHILA; human; signaling; Hematopoiesis; MODEL ORGANISM}, year = {2021}, eissn = {1097-4652}, pages = {5592-5619} } @article{MTMT:31871641, title = {The preference of Trichopria drosophilae for pupae of Drosophila suzukii is independent of host size}, url = {https://m2.mtmt.hu/api/publication/31871641}, author = {Häussling, B.J.M. and Lienenlüke, J. and Stökl, J.}, doi = {10.1038/s41598-020-80355-5}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {11}, unique-id = {31871641}, issn = {2045-2322}, abstract = {Controlling the cosmopolitan pest Drosophila suzukii (spotted wing drosophila) is a challenge for fruit growers. A promising agent for biological control of that pest are parasitoid wasps. Especially the widespread pupal parasitoid Trichopria drosophilae had shown the ability to parasitise the pest fly. However, as a biocontrol agent, parasitoids can only be effective when they prefer the pest to other insects. Until now studies have been inconsistent concerning the preference of T. drosophilae for D. suzukii and whether the preference depends on pupal volume. To clarify this inconsistency, we used video recordings of parasitisation experiments with a set up to observe the direct host preference of the parasitoid. Additionally, the volume of each host pupa was measured. We found significant preference of T. drosophilae for D. suzukii pupae independent of the pupal size and of the host species the wasps were reared on. The article also discusses the sex ratio and the success of the parasitoid in the different pupae characteristics. © 2021, The Author(s).}, keywords = {Female; Male; ARTICLE; sex ratio; nonhuman; videorecording; Parasitoid; biological control agent; WASP; Pupa; Drosophila suzukii}, year = {2021}, eissn = {2045-2322} } @article{MTMT:32006156, title = {Macrophages and Their Organ Locations Shape Each Other in Development and Homeostasis – A Drosophila Perspective}, url = {https://m2.mtmt.hu/api/publication/32006156}, author = {Mase, A. and Augsburger, J. and Brückner, K.}, doi = {10.3389/fcell.2021.630272}, journal-iso = {FRONT CELL DEV BIOL}, journal = {FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY}, volume = {9}, unique-id = {32006156}, issn = {2296-634X}, abstract = {Across the animal kingdom, macrophages are known for their functions in innate immunity, but they also play key roles in development and homeostasis. Recent insights from single cell profiling and other approaches in the invertebrate model organism Drosophila melanogaster reveal substantial diversity among Drosophila macrophages (plasmatocytes). Together with vertebrate studies that show genuine expression signatures of macrophages based on their organ microenvironments, it is expected that Drosophila macrophage functional diversity is shaped by their anatomical locations and systemic conditions. In vivo evidence for diverse macrophage functions has already been well established by Drosophila genetics: Drosophila macrophages play key roles in various aspects of development and organogenesis, including embryogenesis and development of the nervous, digestive, and reproductive systems. Macrophages further maintain homeostasis in various organ systems and promote regeneration following organ damage and injury. The interdependence and interplay of tissues and their local macrophage populations in Drosophila have implications for understanding principles of organ development and homeostasis in a wide range of species. © Copyright © 2021 Mase, Augsburger and Brückner.}, keywords = {regeneration; MACROPHAGE; Development; Drosophila melanogaster; Homeostasis; Hemocyte; Plasmatocyte; organ microenvironment}, year = {2021}, eissn = {2296-634X} } @article{MTMT:32006123, title = {Signaling cross-talk during development: Context-specific networking of Notch, NF-κB and JNK signaling pathways in Drosophila}, url = {https://m2.mtmt.hu/api/publication/32006123}, author = {Mishra, A.K. and Sharma, V. and Mutsuddi, M. and Mukherjee, A.}, doi = {10.1016/j.cellsig.2021.109937}, journal-iso = {CELL SIGNAL}, journal = {CELLULAR SIGNALLING}, volume = {82}, unique-id = {32006123}, issn = {0898-6568}, abstract = {Multicellular organisms depend on a handful of core signaling pathways that regulate a variety of cell fate choices. Often these relatively simple signals integrate to form a large and complex signaling network to achieve a distinct developmental fate in a context-specific manner. Various pathway-dependent and independent events control the assembly of signaling complexes. Notch pathway is one such conserved signaling mechanism that integrates with other signaling pathways to exhibit a context-dependent pleiotropic output. To understand how Notch signaling provides a spectrum of distinct outputs, it is important to understand various regulatory switches involved in mediating signaling cross-talk of Notch with other pathways. Here, we review our current understanding as to how Notch signal integrates with JNK and NF-κB signaling pathways in Drosophila to regulate various developmental events such as sensory organ precursor formation, innate immunity, dorsal closure, establishment of planar cell polarity as well as during proliferation and tumor progression. We highlight the importance of conserved signaling molecules during these cross-talks and debate further possibilities of novel switches that may be involved in mediating these cross-talk events. © 2021 Elsevier Inc.}, keywords = {DROSOPHILA; NF-κB; Cross-talk; Notch signaling; JNK signaling}, year = {2021}, eissn = {1873-3913} } @article{MTMT:32876736, title = {Extracellular matrix protein N-glycosylation mediates immune self-tolerance in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/32876736}, author = {Mortimer, N.T. and Fischer, M.L. and Waring, A.L. and Pooja, K.R. and Kacsoh, B.Z. and Brantley, S.E. and Keebaugh, E.S. and Hill, J. and Lark, C. and Martin, J. and Bains, P. and Lee, J. and Vrailas-Mortimer, A.D. and Schlenke, T.A.}, doi = {10.1073/pnas.2017460118}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {118}, unique-id = {32876736}, issn = {0027-8424}, abstract = {In order to respond to infection, hosts must distinguish pathogens from their own tissues. This allows for the precise targeting of immune responses against pathogens and also ensures self-tolerance, the ability of the host to protect self tissues from immune damage. One way to maintain self-tolerance is to evolve a self signal and suppress any immune response directed at tissues that carry this signal. Here, we characterize the Drosophila tuSz1 mutant strain, which mounts an aberrant immune response against its own fat body. We demonstrate that this autoimmunity is the result of two mutations: 1) a mutation in the GCS1 gene that disrupts N-glycosylation of extracellular matrix proteins covering the fat body, and 2) a mutation in the Drosophila Janus Kinase ortholog that causes precocious activation of hemocytes. Our data indicate that N-glycans attached to extracellular matrix proteins serve as a self signal and that activated hemocytes attack tissues lacking this signal. The simplicity of this invertebrate self-recognition system and the ubiquity of its constituent parts suggests it may have functional homologs across animals. © 2021 National Academy of Sciences. All rights reserved.}, keywords = {Animals; Adult; Female; Male; GENE; MUTATION; MUTATION; metabolism; GENETICS; ARTICLE; HEMOCYTES; immunology; animal; growth, development and aging; innate immunity; gene mutation; controlled study; nonhuman; Drosophila melanogaster; Drosophila melanogaster; immunological tolerance; immunological tolerance; cell activation; immune response; blood cell; glycosylation; glycosylation; Autoimmunity; Autoimmunity; Extracellular Matrix Proteins; Immune Tolerance; scleroprotein; scleroprotein; Janus kinase; Janus kinase; Janus Kinases; protein glycosylation; Self Tolerance; Drosophila Proteins; Drosophila protein; FAT PAD; SELF-TOLERANCE; self-recognition; protein N-glycosylation; GCS1 gene}, year = {2021}, eissn = {1091-6490} } @article{MTMT:32601796, title = {Activin and BMP Signaling Activity Affects Different Aspects of Host Anti-Nematode Immunity in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/32601796}, author = {Ozakman, Y. and Raval, D. and Eleftherianos, I.}, doi = {10.3389/fimmu.2021.795331}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {12}, unique-id = {32601796}, issn = {1664-3224}, abstract = {The multifaceted functions ranging from cellular and developmental mechanisms to inflammation and immunity have rendered TGF-ß signaling pathways as critical regulators of conserved biological processes. Recent studies have indicated that this evolutionary conserved signaling pathway among metazoans contributes to the Drosophila melanogaster anti-nematode immune response. However, functional characterization of the interaction between TGF-ß signaling activity and the mechanisms activated by the D. melanogaster immune response against parasitic nematode infection remains unexplored. Also, it is essential to evaluate the precise effect of entomopathogenic nematode parasites on the host immune system by separating them from their mutualistic bacteria. Here, we investigated the participation of the TGF-ß signaling branches, activin and bone morphogenetic protein (BMP), to host immune function against axenic or symbiotic Heterorhabditis bacteriophora nematodes (parasites lacking or containing their mutualistic bacteria, respectively). Using D. melanogaster larvae carrying mutations in the genes coding for the TGF-ß extracellular ligands Daw and Dpp, we analyzed the changes in survival ability, cellular immune response, and phenoloxidase (PO) activity during nematode infection. We show that infection with axenic H. bacteriophora decreases the mortality rate of dpp mutants, but not daw mutants. Following axenic or symbiotic H. bacteriophora infection, both daw and dpp mutants contain only plasmatocytes. We further detect higher levels of Dual oxidase gene expression in dpp mutants upon infection with axenic nematodes and Diptericin and Cecropin gene expression in daw mutants upon infection with symbiotic nematodes compared to controls. Finally, following symbiotic H. bacteriophora infection, daw mutants have higher PO activity relative to controls. Together, our findings reveal that while D. melanogaster Dpp/BMP signaling activity modulates the DUOX/ROS response to axenic H. bacteriophora infection, Daw/activin signaling activity modulates the antimicrobial peptide and melanization responses to axenic H. bacteriophora infection. Results from this study expand our current understanding of the molecular and mechanistic interplay between nematode parasites and the host immune system, and the involvement of TGF-ß signaling branches in this process. Such findings will provide valuable insight on the evolution of the immune role of TGF-ß signaling, which could lead to the development of novel strategies for the effective management of human parasitic nematodes. Copyright © 2021 Ozakman, Raval and Eleftherianos.}, keywords = {Inflammation; GENE; ARTICLE; signal transduction; BONE MORPHOGENETIC PROTEIN; DROSOPHILA; innate immunity; nonhuman; enzyme activity; immune system; Gene Expression; PROTEIN FUNCTION; cellular immunity; immune response; reactive oxygen metabolite; nitric oxide; plasma cell; HETERORHABDITIS; reduced nicotinamide adenine dinucleotide phosphate oxidase; loss of function mutation; confocal microscopy; Photorhabdus; log rank test; mortality rate; dual oxidase; ACTIVIN; BMP SIGNALING; nematodiasis; TGF-ß}, year = {2021}, eissn = {1664-3224} } @article{MTMT:32365191, title = {Hemocyte RNA-Seq analysis of Indian malarial vectors Anopheles stephensi and Anopheles culicifacies: From similarities to differences}, url = {https://m2.mtmt.hu/api/publication/32365191}, author = {Rani, Jyoti and Chauhan, Charu and Das, De Tanwee and Kumari, Seena and Sharma, Punita and Tevatiya, Sanjay and Patel, Karan and Mishra, K. Ashwani and Pandey, C. Kailash and Singh, Namita and Dixit, Rajnikant}, doi = {10.1016/j.gene.2021.145810}, journal-iso = {GENE}, journal = {GENE}, volume = {798}, unique-id = {32365191}, issn = {0378-1119}, abstract = {Anopheles stephensi and Anopheles culicifacies are dominant malarial vectors in urban and rural India, respectively. Both species carry significant biological differences in their behavioral adaptation and immunity, but the genetic basis of these variations are still poorly understood. Here, we uncovered the genetic differences of immune blood cells, that influence several immune-physiological responses. We generated, analyzed and compared the hemocyte RNA-Seq database of both mosquitoes. A total of 5,837,223,769 assembled bases collapsed into 7,595 and 3,791 transcripts, originating from hemocytes of laboratory-reared 3-4 days old na & iuml;ve (sugar-fed) mosquitoes, Anopheles stephensi and Anopheles culicifacies respectively. Comparative GO annotation analysis revealed that both mosquito hemocytes encode similar proteins. Furthermore, while An. stephensi hemocytes showed a higher percentage of immune transcripts encoding APHAG (Autophagy), IMD (Im-mune deficiency pathway), PRDX (Peroxiredoxin), SCR (Scavenger receptor), IAP (Inhibitor of apoptosis), GALE (galactoside binding lectins), BGBPs (1,3 beta D glucan binding proteins), CASPs (caspases) and SRRP (Small RNA regulatory pathway), An. culicifacies hemocytes yielded a relatively higher percentage of transcripts encoding CLIP (Clip domain serine protease), FREP (Fibrinogen related proteins), PPO (Prophenol oxidase), SRPN (Serpines), ML (Myeloid differentiation 2-related lipid recognition protein), Toll path and TEP (Thioester protein), family proteins. However, a detailed comparative Interproscan analysis showed An. stephensi mosquito hemocytes encode proteins with increased repeat numbers as compared to An. culicifacies. Notably, we observed an abundance of transcripts showing significant variability of encoded proteins with repeats such as LRR (Leucine rich repeat), WD40 (W-D dipeptide), Ankyrin, Annexin, Tetratricopeptide and Mito-chondrial substrate carrier repeat-containing family proteins, which may have a direct influence on species-specific immune-physiological responses. Summarily, our deep sequencing analysis unraveled that An. stephensi evolved with an expansion of repeat sequences in hemocyte proteins as compared to An. culicifacies, possibly providing an advantage for better adaptation to diverse environments.}, year = {2021}, eissn = {1879-0038}, orcid-numbers = {Singh, Namita/0000-0002-6782-4288} } @article{MTMT:32023128, title = {Regression plane concept for analysing continuous cellular processes with machine learning}, url = {https://m2.mtmt.hu/api/publication/32023128}, author = {Szkalisity, Ábel and Piccinini, Filippo and Beleon, Attila and Balassa, Tamás and Varga, Gergely István and Migh, Ede and Molnár, Csaba and Paavolainen, Lassi and Timonen, Sanna and Banerjee, Indranil and Ikonen, Elina and Yamauchi, Yohei and Andó, István and Peltonen, Jaakko and Pietiäinen, Vilja and Honti, Viktor and Horváth, Péter}, doi = {10.1038/s41467-021-22866-x}, journal-iso = {NAT COMMUN}, journal = {NATURE COMMUNICATIONS}, volume = {12}, unique-id = {32023128}, issn = {2041-1723}, year = {2021}, eissn = {2041-1723}, orcid-numbers = {Piccinini, Filippo/0000-0002-0371-7782; Varga, Gergely István/0000-0001-9073-5788; Molnár, Csaba/0000-0002-6124-1209; Ikonen, Elina/0000-0001-8382-1135; Yamauchi, Yohei/0000-0002-8233-9133; Andó, István/0000-0002-4648-9396; Pietiäinen, Vilja/0000-0003-3125-2406} } @article{MTMT:32601797, title = {Drosophila melanogaster larva injection protocol}, url = {https://m2.mtmt.hu/api/publication/32601797}, author = {Tafesh-Edwards, G. and Kenney, E. and Eleftherianos, I.}, doi = {10.3791/63144}, journal-iso = {JOVE-J VIS EXP}, journal = {JOVE-JOURNAL OF VISUALIZED EXPERIMENTS}, unique-id = {32601797}, issn = {1940-087X}, abstract = {The use of unconventional models to study innate immunity and pathogen virulence provides a valuable alternative to mammalian models, which can be costly and raise ethical issues. Unconventional models are notoriously cheap, easy to handle and culture, and do not take much space. They are genetically amenable and possess complete genome sequences, and their use presents no ethical considerations. The fruit fly Drosophila melanogaster, for instance, has provided great insights into a variety of behavior, development, metabolism, and immunity research. More specifically, D. melanogaster adult flies and larvae possess several innate defense reactions that are shared with vertebrate animals. The mechanisms regulating immune responses have been mostly revealed through genetic and molecular studies in the D. melanogaster model. Here a novel larval injection technique is provided, which will further promote investigations of innate immune processes in D. melanogaster larvae and explore the pathogenesis of a wide range of microbial infections. © 2021 JoVE Journal of Visualized Experiments.}, year = {2021}, eissn = {1940-087X} } @article{MTMT:31868552, title = {Immune cell production is targeted by parasitoid wasp virulence in a drosophila–parasitoid wasp interaction}, url = {https://m2.mtmt.hu/api/publication/31868552}, author = {Trainor, J.E. and Pooja, K.R. and Mortimer, N.T.}, doi = {10.3390/pathogens10010049}, journal-iso = {PATHOGENS}, journal = {PATHOGENS}, volume = {10}, unique-id = {31868552}, abstract = {The interactions between Drosophila melanogaster and the parasitoid wasps that infect Drosophila species provide an important model for understanding host–parasite relationships. Following parasitoid infection, D. melanogaster larvae mount a response in which immune cells (hemocytes) form a capsule around the wasp egg, which then melanizes, leading to death of the parasitoid. Previous studies have found that host hemocyte load; the number of hemocytes available for the encapsulation response; and the production of lamellocytes, an infection induced hemocyte type, are major determinants of host resistance. Parasitoids have evolved various virulence mechanisms to overcome the immune response of the D. melanogaster host, including both active immune suppression by venom proteins and passive immune evasive mechanisms. We identified a previously undescribed parasitoid species, Asobara sp. AsDen, which utilizes an active virulence mechanism to infect D. melanogaster hosts. Asobara sp. AsDen infection inhibits host hemocyte expression of msn, a member of the JNK signaling pathway, which plays a role in lamellocyte production. Asobara sp. AsDen infection restricts the production of lamellocytes as assayed by hemocyte cell morphology and altered msn expression. Our findings suggest that Asobara sp. AsDen infection alters host signaling to suppress immunity. © 2021 by the authors. Li-censee MDPI, Basel, Switzerland.}, keywords = {Female; ARTICLE; VENOM; signal transduction; PHYLOGENY; DROSOPHILA; DROSOPHILA; controlled study; nonhuman; sequence analysis; Drosophila melanogaster; immunocompetent cell; encapsulation; immune response; blood cell; principal component analysis; cell structure; immunosuppressive treatment; bacterial virulence; passive immunization; DNA extraction; Lamellocyte; Parasitoid; Sanger sequencing; Parasitoid wasp; Immune cell; Virulence strategy; hemolymphatic system}, year = {2021}, eissn = {2076-0817}, pages = {1-16} } @article{MTMT:32302569, title = {Morphological Characterisation of Haemocytes in the Mealworm Beetle Tenebrio molitor (Coleoptera, Tenebrionidae)}, url = {https://m2.mtmt.hu/api/publication/32302569}, author = {Vommaro, Maria Luigia and Kurtz, Joachim and Giglio, Anita}, doi = {10.3390/insects12050423}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {12}, unique-id = {32302569}, abstract = {Simple SummaryTenebrio molitor is a pest of stored grain, causing considerable damage. However, its easy maintenance makes this species also interesting as a food source and as a model for physiological, immunological, ecological and evolutionary studies. We used light and transmission electron microscopy to study the morphology of circulating haemocytes. Prohaemocytes, plasmatocytes, granular cells and oenocytoids were described based on their morphological features and staining affinity. Results are a baseline for further study aimed to clarify the structure and function of haemocytes in insects.The immunocompetence of the mealworm beetle Tenebrio molitor has been well investigated at molecular and physiological levels, but information on morphological and functional characteristics of its immune cells (haemocytes) is still scarce and fragmentary. This study provides an updated overview of the morphology of circulating immune cells from mealworm beetle adults, using light and transmission electron microscopy. Based on their affinities for May-Grunwald Giemsa stain, haemocytes were defined as either eosinophilic, basophilic or neutral. Ultrastructural descriptions allowed to detect four main cell types in the haemolymph: prohaemocytes, plasmatocytes, granular cells and oenocytoids. The morphological plasticity of haemocytes and the evidence of mitotic circulating cells, intermediate cell stages, as well as autophagic activities suggest haemocyte proliferation, turnover and transdifferentiation as constantly active processes in the haemolymph. Cytochemical tests revealed differences in the distribution of carbohydrates among cell types underling the great plasticity of the immune response and the direct involvement of circulating immune cells in the resource allocation. In addition, our results provide a detailed morphological description of vesicle trafficking, macro- and microautophagy, apoptotic and necrotic processes, confirming the suitability of T. molitor haemocytes as a model for studying evolutionarily conserved cellular mechanisms.}, keywords = {APOPTOSIS; electron microscopy; Mitosis; Autophagy; exosome; Vesicular trafficking; insect immunity; haemocyte morphology}, year = {2021}, eissn = {2075-4450}, orcid-numbers = {Vommaro, Maria Luigia/0000-0003-0921-8260; Giglio, Anita/0000-0001-6513-5027} } @article{MTMT:31871642, title = {Chitin binding protein from the kuruma shrimp Marsupenaeus japonicus facilitates the clearance of Vibrio anguillarum}, url = {https://m2.mtmt.hu/api/publication/31871642}, author = {Xu, S. and Jing, M. and Kong, D.-M. and Wang, Y.-R. and Zhou, Q. and Liu, W.-Y. and Jiao, F. and Li, Y.-J. and Xie, S.-Y.}, doi = {10.1016/j.dci.2020.103981}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {117}, unique-id = {31871642}, issn = {0145-305X}, abstract = {Peritrophic membrane (PM) refers to a vital physical barrier enabling shrimp to resist pathogen invasion. It primarily consists of chitin and proteins, mostly chitin-binding protein (CBP). CBPs have been identified from microorganisms to higher organisms. In the present study, a CBP, designated MjCBP, was reported from Marsupenaeus japonicus. The open reading frame of MjCBP was 1854 bp, encoding a protein with 618 amino acids (MH544098). To be specific, the theoretical pI and molecular mass of mature MjCBP reached 5.43 and 66064.00 Da, respectively. MjCBP consisted of seven type Ⅱ chitin-binding domains (ChtB D2), which was up-regulated after being challenged with Vibrio anguillarum and then agglutinating several bacteria. In addition, MjCBP and the first chitin-binding domain (CBD1) could bind to several Gram-positive and Gram-negative bacteria via the binding process to lipopolysaccharides and peptidoglycans, whereas CBD1 was not capable of agglutinating bacteria. Moreover, the anterior and posterior segments of CBD1 were synthesized in vitro, and the posterior segment could bind to lipopolysaccharides. However, both segments fail to agglutinate bacteria. Furthermore, MjCBP and CBD1 facilitated the clearance of V. anguillarum in vivo, and the silencing of MjCBP via RNA interference reduced the ability of bacterial clearance. As revealed from the mentioned results, MjCBP acts as an opsonin or pattern recognition receptor to achieve antibacterial immune response in shrimp. © 2020}, keywords = {innate immunity; Marsupenaeus japonicus; chitin-binding protein; peritrophic membrane; Antibacterial response}, year = {2021}, eissn = {1879-0089} } @article{MTMT:32361877, title = {Methotrexate negatively acts on inflammatory responses triggered in Drosophila larva with hyperactive JAK/STAT pathway}, url = {https://m2.mtmt.hu/api/publication/32361877}, author = {Yadav, Ravi Kant and Gautam, Dushyant Kumar and Muj, Chukhu and Balija, Madhu Babu Gajula and Paddibhatla, Indira}, doi = {10.1016/j.dci.2021.104161}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {123}, unique-id = {32361877}, issn = {0145-305X}, abstract = {Drosophila is a valuable paradigm for studying tumorigenesis and cancer. Mutations causing hematopoietic aberrations and melanotic-blood-tumors found in Drosophila mutants are vastly studied. Clear understanding about the blood cells, signaling pathways and the tissues affected during hematopoietic tumor formation provide an opportunity to delineate the effects of cancer therapeutics. Using this simple hematopoietic archetype, we elucidated the effects of the anti-cancer drug, Methotrexate (MTX) on immune responses in two scenarios i.e. against wasp infection and in hematopoietic mutant, hopTum-l. Through this in vivo study we show that MTX impedes the immune responses against wasp infection including the encapsulation response. We further observed that MTX reduces the tumor penetrance in gain-of-function mutants of JAK/STAT pathway, hopTum-l. MTX is antiinflammatory as it hinders not only the immune responses of acute inflammation as observed after wasp infestation, but also chronic inflammatory responses associated with constitutively activated JAK/STAT pathway mutant (hopTum-l) carrying blood tumors.}, keywords = {Inflammation; encapsulation; Hematopoiesis; lamellocytes; MTX; Blood tumor}, year = {2021}, eissn = {1879-0089} } @article{MTMT:31871643, title = {Immune priming in shellfish: A review and an updating mechanistic insight focused on cellular and humoral responses}, url = {https://m2.mtmt.hu/api/publication/31871643}, author = {Yang, W. and Tran, N.T. and Zhu, C.-H. and Yao, D.-F. and Aweya, J.J. and Gong, Y. and Ma, H.-Y. and Zhang, Y.-L. and Li, G.-L. and Li, S.-K.}, doi = {10.1016/j.aquaculture.2020.735831}, journal-iso = {AQUACULTURE}, journal = {AQUACULTURE}, volume = {530}, unique-id = {31871643}, issn = {0044-8486}, abstract = {Immune priming has now been demonstrated as a form of immune memory in a wide range of invertebrate species that lack acquired immune systems. Owing to the specific activation of innate immunity upon reinfection, immune priming has important implications for vaccinating economically valuable shellfish to effectively prevent infectious diseases. To gain a clearer understanding of the underlying mechanisms, extensive research has recently been concentrated on priming responses and better evidence-based mechanistic explanations appear as a prerequisite for the development of prophylactic strategies. This review specially focused on cellular and humoral responses to examine the evidence and inquire into the possible mechanisms involved in broad-spectrum or specific immune priming with particular reference to shellfish, with the aims of providing an overview of the current status of research and helping us better understand the existing knowledge. Current data suggest that both humoral and cellular immune factors are to some extent involved in priming protection, but it is far more likely that cellular immunity is more important in terms of the clearance of pathogens and survival. Antimicrobial peptides and other humoral factors seem to be unable to generate priming specificity, but the only selectivity. Alternatively, the elevated phagocytosis of hemocytes could be linked to the specific recognition of antigens in primed hosts. The molecular mechanism appears to rely upon certain receptors/effectors diversified via alternative splicing that could produce splice variants with specific binding capabilities. Our review concludes that whilst immune priming justifies the theoretical feasibility of ‘vaccination’ for bacterial and viral disease control, the priming effect and its impact on immune modulation remain to be further clarified. The practicality of vaccine strategy, particularly the benefit and efficiency, also needs reevaluations in the future. © 2020 Elsevier B.V.}, keywords = {PHAGOCYTOSIS; PEPTIDE; MEMORY; Bacteria (microorganisms); PATHOGEN; Immunity; infectious disease; invertebrate; immune system; immune response; ANTIMICROBIAL ACTIVITY; Invertebrata; Shellfish; cell component; Innate immune memory; Hemocyte proliferation; Immune specificity}, year = {2021}, eissn = {1873-5622} } @article{MTMT:31468836, title = {Maternal Priming of Offspring Immune System in Drosophila}, url = {https://m2.mtmt.hu/api/publication/31468836}, author = {Bozler, Julianna and Kacsoh, Balint Z. and Bosco, Giovanni}, doi = {10.1534/g3.119.400852}, journal-iso = {G3-GENES GENOM GENET}, journal = {G3-GENES GENOMES GENETICS}, volume = {10}, unique-id = {31468836}, issn = {2160-1836}, abstract = {Immune priming occurs when a past infection experience leads to a more effective immune response upon a secondary exposure to the infection or pathogen. In some instances, parents are able to transmit immune priming to their offspring, creating a subsequent generation with a superior immune capability, through processes that are not yet fully understood. Using a parasitoid wasp, which infects larval stages of Drosophila melanogaster, we describe an example of an intergenerational inheritance of immune priming. This phenomenon is anticipatory in nature and does not rely on parental infection, but rather, when adult fruit flies are cohabitated with a parasitic wasp, they produce offspring that are more capable of mounting a successful immune response against a parasitic macro-infection. This increase in offspring survival correlates with a more rapid induction of lamellocytes, a specialized immune cell. RNA-sequencing of the female germline identifies several differentially expressed genes following wasp exposure, including the peptiodoglycan recognition protein-LB (PGRP-LB). We find that genetic manipulation of maternal PGRP-LB identifies this gene as a key element in this intergenerational phenotype.}, keywords = {Immunity; immune priming; intergenerational; transgenerational; leptopilina heterotoma; leptopilina victoriae; PGRP-LB}, year = {2020}, eissn = {2160-1836}, pages = {165-175}, orcid-numbers = {Bosco, Giovanni/0000-0002-8889-9895} } @article{MTMT:31460653, title = {Temporal specificity and heterogeneity ofDrosophilaimmune cells}, url = {https://m2.mtmt.hu/api/publication/31460653}, author = {Cattenoz, Pierre B. and Sakr, Rosy and Pavlidaki, Alexia and Delaporte, Claude and Riba, Andrea and Molina, Nacho and Hariharan, Nivedita and Mukherjee, Tina and Giangrande, Angela}, doi = {10.15252/embj.2020104486}, journal-iso = {EMBO J}, journal = {EMBO JOURNAL}, volume = {39}, unique-id = {31460653}, issn = {0261-4189}, abstract = {Immune cells provide defense against non-self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity ofDrosophilaimmune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single-cell RNA sequencing, we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct processes, e.g., proliferation, phagocytosis, metabolic homeostasis, and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation, which triggers the differentiation of a novel hemocyte type, the lamellocyte. This first molecular atlas of hemocytes provides insights and paves the way to study the biology of theDrosophilaimmune cells in physiological and pathological conditions.}, keywords = {Drosophila melanogaster; immune cells; Single-cell RNA-seq; wasp infestation}, year = {2020}, eissn = {1460-2075} } @article{MTMT:31868487, title = {Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila}, url = {https://m2.mtmt.hu/api/publication/31868487}, author = {Cho, B. and Yoon, S.-H. and Lee, D. and Koranteng, F. and Tattikota, S.G. and Cha, N. and Shin, M. and Do, H. and Hu, Y. and Oh, S.Y. and Lee, D. and Vipin, Menon A. and Moon, S.J. and Perrimon, N. and Nam, J.-W. and Shim, J.}, doi = {10.1038/s41467-020-18135-y}, journal-iso = {NAT COMMUN}, journal = {NATURE COMMUNICATIONS}, volume = {11}, unique-id = {31868487}, issn = {2041-1723}, abstract = {The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution. © 2020, The Author(s).}, keywords = {Animals; metabolism; BLOOD; GENETICS; ARTICLE; RNA; DROSOPHILA; HEMOCYTES; animal; MAP; Cell Differentiation; Cell Differentiation; physiology; Cytology; nonhuman; Host-Parasite Interactions; Immunity; host parasite interaction; pathogenicity; pathology; parasitology; Vertebrata; immune system; Animals, Genetically Modified; Drosophila melanogaster; Drosophila melanogaster; Gene Expression Profiling; Gene Expression Profiling; cellular immunity; immune response; immune response; blood cell; blood cell; transcriptomics; lymphoid tissue; lymphoid tissue; transgenic animal; Cell Lineage; Cell Lineage; Transcriptome; Transcriptome; Transcriptome; lymph node; Hematopoiesis; Hematopoiesis; detection method; Wasps; myeloid progenitor cell; WASP; WASP; Single-Cell Analysis; single cell analysis; RNA-Seq; cells by body anatomy; cell component; single cell RNA seq; Ectoparasitosis; Ectoparasitic Infestations}, year = {2020}, eissn = {2041-1723} } @article{MTMT:30819399, title = {Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid Zaprionus indianus}, url = {https://m2.mtmt.hu/api/publication/30819399}, author = {Cinege, Gyöngyi Ilona and Lerner, Zita and Magyar, Lilla Brigitta and Soós, Bálint and Tóth, Renáta and Kristó, Ildikó and Vilmos, Péter and Juhász, Gábor and Kovács, Attila Lajos and Hegedűs, Zoltán and Sensen, Christoph W. and Kurucz, Judit Éva and Andó, István}, doi = {10.1159/000502646}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {12}, unique-id = {30819399}, issn = {1662-811X}, year = {2020}, eissn = {1662-8128}, pages = {257-272}, orcid-numbers = {Juhász, Gábor/0000-0001-8548-8874; Andó, István/0000-0002-4648-9396} } @article{MTMT:31686151, title = {Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments}, url = {https://m2.mtmt.hu/api/publication/31686151}, author = {Csordás, Gábor and Grawe, Ferdinand and Uhlirova, Mirka}, doi = {10.7554/eLife.57297}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {9}, unique-id = {31686151}, issn = {2050-084X}, abstract = {Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation. The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood. Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments. We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment. We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association. These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila}, year = {2020}, eissn = {2050-084X}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839} } @article{MTMT:31470099, title = {Drosophilamelanogaster Responses against Entomopathogenic Nematodes: Focus on Hemolymph Clots}, url = {https://m2.mtmt.hu/api/publication/31470099}, author = {Dziedziech, Alexis and Shivankar, Sai and Theopold, Ulrich}, doi = {10.3390/insects11010062}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {11}, unique-id = {31470099}, abstract = {Several insect innate immune mechanisms are activated in response to infection by entomopathogenic nematodes (EPNs). In this review, we focus on the coagulation of hemolymph, which acts to stop bleeding after injury and prevent access of pathogens to the body cavity. After providing a general overview of invertebrate coagulation systems, we discuss recent findings in Drosophila melanogaster which demonstrate that clots protect against EPN infections. Detailed analysis at the cellular level provided insight into the kinetics of the secretion of Drosophila coagulation factors, including non-classical modes of secretion. Roughly, clot formation can be divided into a primary phase in which crosslinking of clot components depends on the activity of Drosophila transglutaminase and a secondary, phenoloxidase (PO)-dependent phase, characterized by further hardening and melanization of the clot matrix. These two phases appear to play distinct roles in two commonly used EPN infection models, namely Heterorhabditis bacteriophora and Steinernema carpocapsae. Finally, we discuss the implications of the coevolution between parasites such as EPNs and their hosts for the dynamics of coagulation factor evolution.}, keywords = {TRANSGLUTAMINASE; COAGULATION; SECRETION; HEMOCYTES; innate immunity; Nematodes; clotting; phenoloxidase; insect immunity}, year = {2020}, eissn = {2075-4450}, orcid-numbers = {Dziedziech, Alexis/0000-0001-7647-7639; Theopold, Ulrich/0000-0002-1009-8254} } @article{MTMT:31486658, title = {Thioester-Containing Proteins in the Drosophila melanogaster Immune Response against the Pathogen Photorhabdus}, url = {https://m2.mtmt.hu/api/publication/31486658}, author = {Eleftherianos, Ioannis and Sachar, Upasana}, doi = {10.3390/insects11020085}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {11}, unique-id = {31486658}, abstract = {The fruit fly Drosophila melanogaster forms a magnificent model for interpreting conserved host innate immune signaling and functional processes in response to microbial assaults. In the broad research field of host-microbe interactions, model hosts are used in conjunction with a variety of pathogenic microorganisms to disentangle host immune system activities and microbial pathogenicity strategies. The pathogen Photorhabdus is considered an established model for analyzing bacterial virulence and symbiosis due to its unique life cycle that extends between two invertebrate hosts: an insect and a parasitic nematode. In recent years, particular focus has been given to the mechanistic participation of the D. melanogaster thioester-containing proteins (TEPs) in the overall immune capacity of the fly upon response against the pathogen Photorhabdus alone or in combination with its specific nematode vector Heterorhabditis bacteriophora. The original role of certain TEPs in the insect innate immune machinery was linked to the antibacterial and antiparasite reaction of the mosquito malaria vector Anopheles gambiae; however, revamped interest in the immune competence of these molecules has recently emerged from the D. melanogaster-Photorhabdus infection system. Here, we review the latest findings on this topic with the expectation that such information will refine our understanding of the evolutionary immune role of TEPs in host immune surveillance.}, keywords = {metabolism; INFECTION; Drosophila melanogaster; humoral immunity; cellular immunity; HETERORHABDITIS; Photorhabdus; thioester-containing proteins}, year = {2020}, eissn = {2075-4450}, orcid-numbers = {Eleftherianos, Ioannis/0000-0002-4822-3110} } @article{MTMT:31302837, title = {Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes}, url = {https://m2.mtmt.hu/api/publication/31302837}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordás, Gábor and Rusvai, Miklós and Honti, Viktor and Kolics, Balázs and Török, Tibor and Williams, Michael J and Kurucz, Judit Éva and Andó, István}, doi = {10.1016/j.dci.2020.103701}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {109}, unique-id = {31302837}, issn = {0145-305X}, abstract = {Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes.}, keywords = {Immunity; monoclonal antibody; Hemocyte; Apis mellifera; Honey bee; insect immunity}, year = {2020}, eissn = {1879-0089}, pages = {103701-103706}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Török, Tibor/0000-0002-2128-1126; Andó, István/0000-0002-4648-9396} } @article{MTMT:33777424, title = {Constitutive activation of cellular immunity underlies the evolution of resistance to infection in drosophila}, url = {https://m2.mtmt.hu/api/publication/33777424}, author = {Leitão, A.B. and Arunkumar, R. and Day, J.P. and Geldman, E.M. and Morin-Poulard, I. and Crozatier, M. and Jiggins, F.M.}, doi = {10.7554/ELIFE.59095}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {9}, unique-id = {33777424}, issn = {2050-084X}, abstract = {Organisms rely on inducible and constitutive immune defences to combat infection. Constitutive immunity enables a rapid response to infection but may carry a cost for uninfected individuals, leading to the prediction that it will be favoured when infection rates are high. When we exposed populations of Drosophila melanogaster to intense parasitism by the parasitoid wasp Leptopilina boulardi, they evolved resistance by developing a more reactive cellular immune response. Using single-cell RNA sequencing, we found that immune-inducible genes had become constitutively upregulated. This was the result of resistant larvae differentiating precursors of specialized immune cells called lamellocytes that were previously only produced after infection. Therefore, populations evolved resistance by genetically hard-wiring the first steps of an induced immune response to become constitutive. © 2020, eLife Sciences Publications Ltd. All rights reserved.}, keywords = {Animals; Female; Female; Male; Male; GENETICS; ARTICLE; EVOLUTION; DROSOPHILA; HEMOCYTES; immunology; animal; gene expression regulation; gene expression regulation; nonhuman; larva; larva; Infections; parasitology; Drosophila melanogaster; Drosophila melanogaster; DISEASE RESISTANCE; DISEASE RESISTANCE; human cell; immunocompetent cell; cellular immunity; cellular immunity; immune response; blood cell; Immunity, Cellular; Biological Evolution; Wasps; WASP; single cell RNA seq}, year = {2020}, eissn = {2050-084X}, pages = {1-24} } @article{MTMT:31486671, title = {Trypanosomatid parasite dynamically changes the transcriptome during infection and modifies honey bee physiology}, url = {https://m2.mtmt.hu/api/publication/31486671}, author = {Liu, Qiushi and Lei, Jing and Darby, Alistair C. and Kadowaki, Tatsuhiko}, doi = {10.1038/s42003-020-0775-x}, journal-iso = {COMMUN BIOL}, journal = {COMMUNICATIONS BIOLOGY}, volume = {3}, unique-id = {31486671}, abstract = {It is still not understood how honey bee parasite changes the gene expression to adapt to the host environment and how the host simultaneously responds to the parasite infection by modifying its own gene expression. To address this question, we studied a trypanosomatid, Lotmaria passim, which can be cultured in medium and inhabit the honey bee hindgut. We found that L. passim decreases mRNAs associated with protein translation, glycolysis, detoxification of radical oxygen species, and kinetoplast respiratory chain to adapt to the anaerobic and nutritionally poor honey bee hindgut during the infection. After the long term infection, the host appears to be in poor nutritional status, indicated by the increase and decrease of take-out and vitellogenin mRNAs, respectively. Simultaneous gene expression profiling of L. passim and honey bee during infection by dual RNA-seq provided insight into how both parasite and host modify their gene expressions. Qiushi Liu et al. study gene expression profiles of honey bees and a known honey bee-trypanosomatid parasite. They show that during infection both the host and the parasite modify their gene expression profiles, shedding light into the host-parasite interactions of infected bees.}, year = {2020}, eissn = {2399-3642} } @article{MTMT:31486666, title = {The Intruding Wolbachia Strain from the Moth Fails to Establish Itself in the Fruit Fly Due to Immune and Exclusion Reactions}, url = {https://m2.mtmt.hu/api/publication/31486666}, author = {Liu, Xin-Chao and Li, Yue-Ru and Dong, Bei and Li, Zheng-Xi}, doi = {10.1007/s00284-020-02067-3}, journal-iso = {CURR MICROBIOL}, journal = {CURRENT MICROBIOLOGY}, volume = {77}, unique-id = {31486666}, issn = {0343-8651}, abstract = {Wolbachia is capable of regulating host reproduction, and thus of great significance in preventing the spread of insect-borne diseases and controlling pest insects. The fruit fly Drosophila melanogaster is an excellent model insect for understanding Wolbachia-host interactions. Here we artificially transferred the wCcep strain from the rice moth Corcyra cephalonica into D. melanogaster by microinjection. Crossing experiments indicated that wCcep could induce a high level of CI in the phylogenetically distant host D. melanogaster and imposed no negative fitness costs on host development and fecundity. Based on quantitative analysis, the titres of wCcep and the native wMel strain were negatively correlated, and wCcep could only be transmitted in the novel host for several generations (G(0) to G(4)) after transinfection. Transcriptome sequencing indicated that the invading wCcep strain induced a significant immune- and stress-related response from the host. An association analysis between the expression of immune genes attacin-D/edin and the titre of Wolbachia by linear regression displayed a negative correlation between them. Our study suggest that the intrusion of wCcep elicited a robust immune response from the host and incurred a competitive exclusion from the native Wolbachia strain, which resulted in the failure of its establishment in D. melanogaster.}, year = {2020}, eissn = {1432-0991}, pages = {2441-2448} } @article{MTMT:31469236, title = {The adipokine NimrodB5 regulates peripheral hematopoiesis in Drosophila}, url = {https://m2.mtmt.hu/api/publication/31469236}, author = {Ramond, Elodie and Petrignani, Bianca and Dudzic, Jan Paul and Boquete, Jean-Philippe and Poidevin, Mickael and Kondo, Shu and Lemaitre, Bruno}, doi = {10.1111/febs.15237}, journal-iso = {FEBS J}, journal = {FEBS JOURNAL}, unique-id = {31469236}, issn = {1742-464X}, abstract = {In animals, growth is regulated by the complex interplay between paracrine and endocrine signals. When food is scarce, tissues compete for nutrients, leading to critical resource allocation and prioritization. Little is known about how the immune system maturation is coordinated with the growth of other tissues. Here, we describe a signaling mechanism that regulates the number of hemocytes (blood cells) according to the nutritional state of the Drosophila larva. Specifically, we found that a secreted protein, NimB5, is produced in the fat body upon nutrient scarcity downstream of metabolic sensors and ecdysone signaling. NimB5 is then secreted and binds to hemocytes to down-regulate their proliferation and adhesion. Blocking this signaling loop results in conditional lethality when larvae are raised on a poor diet, due to excessive hemocyte numbers and insufficient energy storage. Similar regulatory mechanisms shaping the immune system in response to nutrient availability are likely to be widespread in animals.}, keywords = {metabolism; GROWTH; DROSOPHILA; TRADE-OFF; Nimrod; organ prioritization; peripheral hematopoiesis}, year = {2020}, eissn = {1742-4658}, orcid-numbers = {Ramond, Elodie/0000-0003-4775-7070; Lemaitre, Bruno/0000-0001-7970-1667} } @article{MTMT:31486669, title = {A novel WSSV responsive plasma protein from Litopenaeus vannamei contributes to hemocytes anti-apoptosis}, url = {https://m2.mtmt.hu/api/publication/31486669}, author = {Tao, Mengyuan and Liang, Yuqin and Zhang, Yueling and Wang, Fan}, doi = {10.1016/j.molimm.2020.02.007}, journal-iso = {MOL IMMUNOL}, journal = {MOLECULAR IMMUNOLOGY}, volume = {120}, unique-id = {31486669}, issn = {0161-5890}, abstract = {Litopenaeus vannamei, as an important marine aquaculture species, has attracted more and more attentions in past several years. More recently people got its genome fine mapping, which unveiled a gene treasure. In this study, we have identified a novel trypsin-like protein which came from previous WSSV-infected shrimp plasma iTRAQ data. This protein is a 39 kDa protein with 363 amino acids. It contains a conserved trypsin-domain and could be strongly induced with WSSV infection. Interestingly, knockdown of this protein made shrimps vulnerable to WSSV infection. Further exploration unveiled that this fragility was probably due to the fact that knockdown of this protein could cause shrimp hemocytes apoptosis, which indicated that this protein played key roles in preventing shrimp hemocytes from apoptosis. To further explore how LvTLAP protected shrimp hemocytes from apoptosis, GST pull down assay was applied to screen LvTLAP interacting protein in shrimp plasma. L. vannamei growth and transformation-dependent-like protein (LvGTD-like protein) was identified as a LvTLAP interacting protein, which played proapoptotic roles in cells. Thus, a possible explanation for LvTLAP anti-apoptosis activity was that this protein could block LvGTD-like protein proapoptotic activity to protect shrimp hemocytes from death. In general, our study has uncovered a novel WSSV responsive shrimp plasma protein, which played key roles in shrimp hemocytes anti-apoptosis and shrimp against WSSV infection.}, keywords = {plasma protein; SHRIMP; anti-apoptosis}, year = {2020}, eissn = {1872-9142}, pages = {113-121} } @article{MTMT:31688125, title = {Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway}, url = {https://m2.mtmt.hu/api/publication/31688125}, author = {Wan, Bin and Poirie, Marylene and Gatti, Jean-Luc}, doi = {10.1080/21505594.2020.1838116}, journal-iso = {VIRULENCE}, journal = {VIRULENCE}, volume = {11}, unique-id = {31688125}, issn = {2150-5594}, abstract = {Venosomes are extracellular vesicles found in the venom of Leptopilina endoparasitoids wasps, which transport and target virulence factors to impair the parasitoid egg encapsulation by the lamellocytes of their Drosophila melanogaster host larva. Using the co-immunolocalization of fluorescent L. boulardi venosomes and one of the putative-transported virulence factors, LbGAP, with known markers of cellular endocytosis, we show that venosomes endocytosis by lamellocytes is not a process dependent on clathrin or macropinocytosis and internalization seems to bypass the early endosomal compartment Rab5. After internalization, LbGAP colocalizes strongly with flotillin-1 and the GPI-anchored protein Atilla/L1 (a lamellocyte surface marker) suggesting that entry occurs via a flotillin/lipid raft-dependent pathway. Once internalized, venosomes reach all intracellular compartments, including late and recycling endosomes, lysosomes, and the endoplasmic reticulum network. Venosomes therefore enter their target cells by a specific mechanism and the virulence factors are widely distributed in the lamellocytes' compartments to impair their functions.}, keywords = {Drosophila melanogaster; Lysosomes; RAFTS; confocal microscopy; Endosomes; Parasitoid wasps; lamellocytes; venosomes}, year = {2020}, eissn = {2150-5608}, pages = {1512-1521} } @article{MTMT:31685719, title = {C-type lectin-mediated microbial homeostasis is critical for Helicoverpa armigera larval growth and development}, url = {https://m2.mtmt.hu/api/publication/31685719}, author = {Wang, Wenwen and Wang, Guijie and Zhuo, Xiaorong and Liu, Yu and Tang, Lin and Liu, Xusheng and Wang, Jialin}, doi = {10.1371/journal.ppat.1008901}, journal-iso = {PLOS PATHOG}, journal = {PLOS PATHOGENS}, volume = {16}, unique-id = {31685719}, issn = {1553-7366}, abstract = {The immune system of a host functions critically in shaping the composition of the microbiota, and some microbes are involved in regulating host endocrine system and development. However, whether the immune system acts on endocrine and development by shaping the composition of the microbiota remains unclear, and few molecular players or microbes involved in this process have been identified. In the current study, we found that RNA interference of a C-type lectin (HaCTL3) in the cotton bollworm Helicoverpa armigera suppresses ecdysone and juvenile hormone signaling, thus reducing larval body size and delaying pupation. Depletion of HaCTL3 also results in an increased abundance of Enterocuccus mundtii in the hemolymph, which may escape from the gut. Furthermore, HaCTL3 and its controlled antimicrobial peptides (attacin, lebocin, and gloverin) are involved in the clearance of E. mundtii from the hemolymph via phagocytosis or direct bactericidal activity. Injection of E. mundtii into larval hemocoel mimics HaCTL3-depleted phenotypes and suppresses ecdysone and juvenile hormone signaling. Taken together, we conclude that HaCTL3 maintains normal larval growth and development of H. armigera via suppressing the abundance of E. mundtii in the hemolymph. Our results provide the first evidence of an immune system acting on an endocrine system to modulate development via shaping the composition of microbiota in insect hemolymph. Thus, this study will deepen our understanding of the interaction between immunity and development.Author summaryConsidering that a large number of hemocytes and multiple soluble effectors are present in insect hemolymph, it is conventionally believed that healthy insect hemolymph is a hostile environment for bacteria and is, therefore, sterile. However, increasing evidences disprove this opinion, although the interactive mechanism between hemolymph microbiota and insect host, as well as the function of hemolymph microbiota, remain unclear. C-type lectin (CTL), as pattern recognition receptor (PRR), plays important roles in defending against various pathogens. Here we found that various bacteria colonized the hemolymph of the cotton bollworm Helicoverpa armigera. We first reported that an H. armigera CTL (HaCTL3) was involved in modulating larval growth and development. Further study indicated that HaCTL3-mediated homeostasis of Enterocuccus mundtii in the hemolymph is critical for normal larval growth and development. Our study demonstrated that this PRR modulated insect development through shaping hemolymph microbiota, which may represent a novel mechanism of immune system regulation during insect development.}, year = {2020}, eissn = {1553-7374} } @article{MTMT:31468662, title = {Differential Expression of Immune Genes between Two Closely Related Beetle Species with Different Immunocompetence following Attack by Asecodes parvidava}, url = {https://m2.mtmt.hu/api/publication/31468662}, author = {Yang, Xuyue and Fors, Lisa and Slotte, Tanja and Theopold, Ulrich and Binzer-Panchal, Mahesh and Wheat, Christopher W. and Hamback, Peter A.}, doi = {10.1093/gbe/evaa075}, journal-iso = {GENOME BIOL EVOL}, journal = {GENOME BIOLOGY AND EVOLUTION}, volume = {12}, unique-id = {31468662}, issn = {1759-6653}, abstract = {Endoparasitoid wasps are important natural enemies of many insect species and are major selective forces on the host immune system. Despite increased interest in insect antiparasitoid immunity, there is sparse information on the evolutionary dynamics of biological pathways and gene regulation involved in host immune defense outside Drosophila species. We de novo assembled transcriptomes from two beetle species and used time-course differential expression analysis to investigate gene expression differences in closely related species Galerucella pusilla and G. calmariensis that are, respectively, resistant and susceptible against parasitoid infection by Asecodes paividava parasitoids. Approximately 271 million and 224 million paired-ended reads were assembled and filtered to form 52,563 and 59,781 transcripts for G. pusilla and G. calmariensis, respectively. In the whole-transcriptome level, an enrichment of functional categories related to energy production, biosynthetic process, and metabolic process was exhibited in both species. The main difference between species appears to be immune response and wound healing process mounted by G. pusilla larvae. Using reciprocal BLAST against the Drosophila melanogaster proteome, 120 and 121 immune-related genes were identified in G. pusilla and G. calmariensis, respectively. More immune genes were differentially expressed in G. pusilla than in G. calmariensis, in particular genes involved in signaling, hematopoiesis, and melanization. In contrast, only one gene was differentially expressed in G. calmariensis. Our study characterizes important genes and pathways involved in different immune functions after parasitoid infection and supports the role of signaling and hematopoiesis genes as key players in host immunity in Galerucella against parasitoid wasps.}, keywords = {Transcriptome; Hematopoiesis; insect immunity; Parasitoid wasp; Galerucella}, year = {2020}, pages = {522-534}, orcid-numbers = {Yang, Xuyue/0000-0003-2084-1651; Slotte, Tanja/0000-0001-6020-5102; Theopold, Ulrich/0000-0002-1009-8254; Binzer-Panchal, Mahesh/0000-0003-1675-0677} } @article{MTMT:31469237, title = {Mitochondrial and Innate Immunity Transcriptomes from Spodoptera frugiperda Larvae Infected with the Spodoptera frugiperda Ascovirus}, url = {https://m2.mtmt.hu/api/publication/31469237}, author = {Zaghloul, Heba A. H. and Hice, Robert and Bideshi, Dennis K. and Arensburger, Peter and Federici, Brian A.}, doi = {10.1128/JVI.01985-19}, journal-iso = {J VIROL}, journal = {JOURNAL OF VIROLOGY}, volume = {94}, unique-id = {31469237}, issn = {0022-538X}, abstract = {Ascoviruses are large, enveloped DNA viruses that induce remarkable changes in cellular architecture during which the cell is partitioned into numerous vesicles for viral replication. Previous studies have shown that these vesicles arise from a process resembling apoptosis yet which differs after nuclear lysis in that mitochondria are not degraded but are modified by the virus, changing in size, shape, and motility. Moreover, infection does not provoke an obvious innate immune response. Thus, we used in vivo RNA sequencing to determine whether infection by the Spodoptera frugiperda ascovirus 1a (SfAV-1a) modified expression of host mitochondrial, cytoskeletal, and innate immunity genes. We show that transcripts from many mitochondrial genes were similar to those from uninfected controls, whereas others increased slightly during vesicle formation, including those for ATP6, ATP8 synthase, and NADH dehydrogenase subunits, supporting electron microscopy (EM) data that these organelles were conserved for virus replication. Transcripts from 58 of 106 cytoskeletal genes studied increased or decreased more than 2-fold postinfection. More than half coded for mitochondrial motor proteins. Similar increases occurred for innate immunity transcripts and their negative regulators, including those for Toll, melanization, and phagocytosis pathways. However, those for many antimicrobial peptides, such as moricin, increased more than 20-fold. In addition, transcripts for gloverin-3, spod_x_tox, Hdd23, and lebocin, also antimicrobial, increased more than 20-fold. Interestingly, a phenoloxidase inhibitor transcript increased 12-fold, apparently to interfere with melanization. SfAV-1a destroys most fat body cells by 7 days postinfection, so innate immunity gene transcripts apparently occur in remaining cells in this tissue and possibly other major tissues, namely, epidermis and tracheal matrix.IMPORTANCE Ascoviruses are large DNA viruses that infect insects, inducing a cellular pathology that resembles apoptosis but which differs by causing enormous cellular hypertrophy followed by cleavage of the cell into numerous viral vesicles for replication. Previous EM studies suggest that mitochondria are important for vesicle formation. Transcriptome analyses of Spodoptera frugiperda larvae infected with SfAV-1a showed that mitochondrial transcripts were similar to those from uninfected controls or increased slightly during vesicle formation, especially for ATP6, ATP8 synthase, and NADH dehydrogenase subunits. This pattern resembles that for chronic disease-inducing viruses, which conserve mitochondria, differing markedly from viruses causing short-term viral diseases, which degrade mitochondrial DNA. Though mitochondrial transcript increases were low, our results demonstrate that SfAV-1a alters host mitochondrial expression more than any other virus. Regarding innate immunity, although SfAV-1a destroys most fat body cells, certain immunity genes were highly upregulated (greater than 20-fold), suggesting that these transcripts may originate from other tissues.}, keywords = {PHAGOCYTOSIS; TOLL; innate immunity; CYTOSKELETON; VESICLE FORMATION; melanization; membrane biogenesis; Ascovirus; Mitochondrial Genes; innate immunity genes; dual transcriptome; dsDNA virus; mitochondrial DNA transcription}, year = {2020}, eissn = {1098-5514} } @article{MTMT:30901165, title = {Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity}, url = {https://m2.mtmt.hu/api/publication/30901165}, author = {Bailetti, Alessandro A. and Negron-Pineiro, Lenny J. and Dhruva, Vishal and Harsh, Sneh and Lu, Sean and Bosula, Aisha and Bach, Erika A.}, doi = {10.1242/dmm.038679}, journal-iso = {DIS MODEL MECH}, journal = {DISEASE MODELS & MECHANISMS}, volume = {12}, unique-id = {30901165}, issn = {1754-8403}, abstract = {Myeloproliferative neoplasms (MPNs) are clonal hematopoietic disorders that cause excessive production of myeloid cells. Most MPN patients have a point mutation in JAK2 (JAK2(V617F)), which encodes a dominant-active kinase that constitutively triggers JAK/STAT signaling. In Drosophila, this pathway is simplified, with a singleJAK, Hopscotch (Hop), and a single STAT transcription factor, Stat92E. The hop(Tumorous-lethal) [hop(Tum)] allele encodes a dominant-active kinase that induces sustained Stat92E activation. Like MPN patients, hop(Tum) mutants have significantly more myeloid cells, which form invasive tumors. Through an unbiased genetic screen, we found that heterozygosity for Enhancer of Polycomb [E(Pc)], a component of the Tip60 lysine acetyltransferase complex (also known as KAT5 in humans), significantly increased tumor burden in hopTum animals. Hematopoietic depletion of E(Pc) or other Tip60 components in an otherwise wild-type background also induced blood cell tumors. The E(Pc) tumor phenotype was dependent on JAK/STAT activity, as concomitant depletion of hop or Stat92E inhibited tumor formation. Stat92E target genes were significantly upregulated in E(Pc)-mutant myeloid cells, indicating that loss of E(Pc) activates JAK STAT signaling. Neither the hop nor Stat92E gene was upregulated upon hematopoietic E(Pc) depletion, suggesting that the regulation of the JAK STAT pathway by E(Pc) is dependent on substrates other than histones. Indeed, E(Pc) depletion significantly increased expression of Hop protein in myeloid cells. This study indicates that E(Pc) works as a tumor suppressor by attenuating Hop protein expression and ultimately JAK STAT signaling. Since loss-of-function mutations in the human homologs of E(Pc) and Tip60 are frequently observed in cancer, our work could lead to new treatments for MPN patients.This article has an associated First Person interview with the first author of the paper.}, keywords = {DROSOPHILA; Tumor suppressor; MYELOPROLIFERATIVE NEOPLASMS; TIP60; JAK/STAT; E(Pc); Melanotic tumors; Lysine acetyltransferases}, year = {2019}, eissn = {1754-8411} } @article{MTMT:30510237, title = {Drosophila as a Genetic Model for Hematopoiesis}, url = {https://m2.mtmt.hu/api/publication/30510237}, author = {Banerjee, Utpal and Girard, Juliet R. and Goins, Lauren M. and Spratford, Carrie M.}, doi = {10.1534/genetics.118.300223}, journal-iso = {GENETICS}, journal = {GENETICS}, volume = {211}, unique-id = {30510237}, issn = {0016-6731}, abstract = {In this FlyBook chapter, we present a survey of the current literature on the development of the hematopoietic system in Drosophila. The Drosophila blood system consists entirely of cells that function in innate immunity, tissue integrity, wound healing, and various forms of stress response, and are therefore functionally similar to myeloid cells in mammals. The primary cell types are specialized for phagocytic, melanization, and encapsulation functions. As in mammalian systems, multiple sites of hematopoiesis are evident in Drosophila and the mechanisms involved in this process employ many of the same molecular strategies that exemplify blood development in humans. Drosophila blood progenitors respond to internal and external stress by coopting developmental pathways that involve both local and systemic signals. An important goal of these Drosophila studies is to develop the tools and mechanisms critical to further our understanding of human hematopoiesis during homeostasis and dysfunction.}, keywords = {DROSOPHILA; innate immunity; Hematopoiesis; stress response; Lamellocyte; Hemocyte; Plasmatocyte; lymph gland; FlyBook; crystal cell}, year = {2019}, eissn = {1943-2631}, pages = {367-417} } @article{MTMT:30908689, title = {JAK/STAT signaling in insect innate immunity}, url = {https://m2.mtmt.hu/api/publication/30908689}, author = {Bang, In Seok}, doi = {10.1111/1748-5967.12384}, journal-iso = {ENTOMOL RES}, journal = {ENTOMOLOGICAL RESEARCH}, volume = {49}, unique-id = {30908689}, issn = {1738-2297}, abstract = {Lack of an adaptive strategy to combat infection creates opportunities for the innate immune system to guide invertebrate defense mechanisms. The innate immunity signaling cascades in invertebrates are elaborate, complex, and pathogen-specific. Among invertebrates, the most extended repertoire of molecules that function in the regulatory signaling pathways has been observed in insects. This is highlighted by the fact that antimicrobial peptide (AMP) production against pathogens is orchestrated through diverse immune pathways, either independently or through cross-talk mechanisms. The Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway coordinates immune responses from cytokines and regulates multiple homeostasis mechanisms in the host. That pathway has been implicated in the regulation of cell growth, differentiation, apoptosis, and inflammatory reactions. Many novel therapeutic interventions for tumors have been aimed at inhibitors of the JAK/STAT cascade. The regulatory pathway has much fewer components in Drosophila, and human homologs of almost all the critical pathway components and negative regulators have been identified. Loss-of-function mutation analysis and RNA interference-based gene silencing modeling have produced functional characterization of the core components and negative regulators in Drosophila melanogaster, Aedes aegypti, and Anopheles gambiae, and in some hymenopteran and lepidopteran species. The genome-wide analysis of the coleopteran species, Tribolium castaneum and Tenebrio molitor have been explored for elucidation of their JAK/STAT pathway regulatory components. Considering the promise of the JAK/STAT pathway in the mammalian model, the regulatory pathway in insects seems interesting especially for understanding pathogen surveillance mechanisms.}, keywords = {Host-Pathogen Interactions; JAK; Antiviral response; insect immunity; SOCS; STAT signaling}, year = {2019}, eissn = {1748-5967}, pages = {339-353} } @article{MTMT:30908690, title = {Harpalus (Pseudoophonus) rufipes as a model to study cellular and humoral immune defence strategies in coleopteran species}, url = {https://m2.mtmt.hu/api/publication/30908690}, author = {Cavaliere, F. and Brandmayr, P. and Giulianini, P. G. and Vommaro, M. L. and Giglio, A.}, journal-iso = {INV SURVIVAL J (ISJ)}, journal = {INVERTEBRATE SURVIVAL JOURNAL}, volume = {16}, unique-id = {30908690}, issn = {1824-307X}, abstract = {Carabids are of special interest as environmental quality assessment indicators of exposure to xenobiotic and for pest control. In agroecosystems, they can be exposed to a wide range of pathogens and environmental pollution exerting a stronger selection on their innate immune systems. Therefore, information on species-specific immunocompetence is necessary to complete the ecological framework of ground beetles. In this study, cellular and humoral responses were characterized in adults of Harpalus (Pseudoophonus) rufipes (De Geer, 1774) to define a baseline knowledge for future ecotoxicological studies. The circulating hemocytes were characterized by light and transmission electron microscopy and in vivo assay performed by injecting latex beads to identify phagocytizing hemocytes. Ultrastructural analyses revealed four morphologically distinct types of circulating hemocytes: prohemocytes, plasmatocytes, granular cells and oenocytoids. Differential hemocyte counts showed that plasmatocytes and granular cells were the most abundant circulating cell types and granular cells exhibited phagocytic activity following immune challenge with latex beads. Mitotic figures and non-differentiated hemocytes observed under light microscopy indicate a continuous cell turnover in the hemolymph. Melanotic nodules found 2h after the immune challenge were formed to immobilize the latex beads. Phenoloxidase (PO) assays revealed an increase of basal PO activity in hemolymph after immune system activation with lipopolysaccharide (LPS). However, the LPS-stimulated adults showed no significant variation in the lysozyme-like enzyme activity in hemolymph. Based on these results, H. rufipes displays a rapid, non-specific immune response involving cellular and humoral effectors that both sequester and clear pathogens.}, keywords = {PHAGOCYTOSIS; HEMOCYTES; ultrastructure; lysozyme; NODULATION; phenoloxidase; Carabid beetles}, year = {2019}, eissn = {1824-307X}, pages = {92-104} } @article{MTMT:30907377, title = {Pericardin, a Drosophila collagen, facilitates accumulation of hemocytes at the heart}, url = {https://m2.mtmt.hu/api/publication/30907377}, author = {Cevik, Duygu and Acker, Meryl and Michalski, Camilla and Jacobs, J. Roger}, doi = {10.1016/j.ydbio.2019.06.006}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {454}, unique-id = {30907377}, issn = {0012-1606}, abstract = {Hematopoietic cell lineages support organismal needs by responding to positional and systemic signals that balance proliferative and differentiation events. Drosophila provides an excellent genetic model to dissect these signals, where the activity of cues in the hemolymph or substrate can be traced to determination and differentiation events of well characterized hemocyte types. Plasmatocytes in third instar larvae increase in number in response to infection and in anticipation of metamorphosis. Here we characterize hemocyte clustering, proliferation and transdifferentiation on the heart or dorsal vessel. Hemocytes accumulate on the inner foldings of the heart basement membrane, where they move with heart contraction, and are in proximity to the heart ostia and pericardial nephrocytes. The numbers of hemocytes vary, but increase transiently before pupariation, and decrease by 4 h before pupa formation. During their accumulation at the heart, plasmatocytes can proliferate and can transdifferentiate into crystal cells. Serrate expressing cells as well as lamellocyte-like, Atilla expressing ensheathing cells are associated with some, but not all hemocyte clusters. Hemocyte aggregation is enhanced by the presence of a heart specific Collagen, Pericardin, but not the associated pericardial cells. The varied and transient number of hemocytes in the pericardial compartment suggests that this is not a hematopoietic hub, but a niche supporting differentiation and rapid dispersal in response to systemic signals.}, keywords = {INFECTION; niche; extracellular matrix; Hematopoiesis; Lamellocyte; Plasmatocyte; dorsal vessel; klf15; Viking. lonely heart}, year = {2019}, eissn = {1095-564X}, pages = {52-65} } @article{MTMT:30908691, title = {The prophenoloxidase system in Drosophila participates in the anti-nematode immune response}, url = {https://m2.mtmt.hu/api/publication/30908691}, author = {Cooper, Dustin and Wuebbolt, Caitlin and Heryanto, Christa and Eleftherianos, Ioannis}, doi = {10.1016/j.molimm.2019.03.008}, journal-iso = {MOL IMMUNOL}, journal = {MOLECULAR IMMUNOLOGY}, volume = {109}, unique-id = {30908691}, issn = {0161-5890}, abstract = {Drosophila melanogaster relies on an evolutionarily conserved innate immune system to protect itself from potentially deadly pathogens. One of the earliest pathways activated after injury or infection is the melanization pathway, which is responsible for synthesizing and depositing melanin at the site of injury, or onto invading microbes. Three genes, PPO1-3, encoding prophenoloxidase (PPO), an inactive precursor of phenoloxidase (PO), are responsible for the production of melanin after their activation via immune challenge. One pathogen capable of infecting D. melanogaster are entomopathogenic nematodes. Steinernema carpocapsae nematodes exist in a mutualistic relationship with Xenorhabdus nematophila bacteria and are an important biological control agent for controlling insect pests. The nematode-bacteria complex (symbiotic nematodes) can be separated, creating "axenic" nematodes, devoid of their associated bacteria, which are still capable of infecting and killing D. melanogaster. In order to investigate how the D. melanogaster melanization pathway contributes to the anti nematode immune response, symbiotic and axenic S. carpocapsae were used to study D. melanogaster survival, PPO gene expression, and activation of PPO to PO. Our research suggests that the expression of all three D. melanogaster PPO genes contributes to survival, however only PPO1 or PPO3 appear to be up-regulated during axenic or symbiotic nematode infection. Additionally, we present data suggesting that a complex regulatory system exists between PPOs, potentially allowing for the compensation of PPOs by one another. Further, we found that axenic nematode infection leads to higher levels of PO, suggesting that X. nematophila suppresses this activation. We also report for the first time the differentiation of lamellocytes, a specialized type of hemocytes in D. melanogaster, in response to symbiotic S. carpocapsae nematode infection. Our results suggest an important role played by the melanization pathway in response to nematode infection, and demonstrate how this response can be manipulated by S. carpocapsae nematodes and their mutualistic X. nematophila bacteria.}, keywords = {DROSOPHILA; innate immunity; Steinernema; prophenoloxidase; Parasitic nematode}, year = {2019}, eissn = {1872-9142}, pages = {88-98} } @article{MTMT:30908366, title = {Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes}, url = {https://m2.mtmt.hu/api/publication/30908366}, author = {Fiorotti, Jessica and Sadok Menna-Barreto, Rubem Figueiredo and Golo, Patricia Silva and Balduino Coutinho-Rodrigues, Caio Junior and Barbosa Bitencourt, Ricardo Oliveira and Spadacci-Morena, Diva Denelle and Angelo, Isabele da Costa and Elias Pinheiro Bittencourt, Vania Rita}, doi = {10.3389/fphys.2019.00654}, journal-iso = {FRONT PHYSIOL}, journal = {FRONTIERS IN PHYSIOLOGY}, volume = {10}, unique-id = {30908366}, abstract = {Metarhizium is an entomopathogenic fungus widely employed in the biological control of arthropods. Hemocytes present in the hemolymph of invertebrates are the cells involved in the immune response of arthropods. Despite this, knowledge about Rhipicephalus microplus hemocytes morphological aspects as well as their role in response to the fungal infection is scarce. The present study aimed to analyze the hemocytes of R. microplus females after Metarhizium robertsii infection, using light and electron microscopy approaches associated with the cytotoxicity evaluation. Five types of hemocytes (prohemocytes, spherulocytes, plasmatocytes, granulocytes, and oenocytoids) were described in the hemolymph of uninfected ticks, while only prohemocytes, granulocytes, and plasmatocytes were observed in fungusinfected tick females. Twenty-four hours after the fungal infection, only granulocytes and plasmatocytes were detected in the transmission electron microscopy analysis. Hemocytes from fungus-infected tick females showed several cytoplasmic vacuoles with different electron densities, and lipid droplets in close contact to low electron density vacuoles, as well as the formation of autophagosomes and subcellular material in different stages of degradation could also be observed. M. robertsii propagules were more toxic to tick hemocytes in the highest concentration tested (1.0 x 10(8) conidia mL(-1)). Interestingly, the lowest fungus concentration did not affect significantly the cell viability. Microanalysis showed that cells granules from fungus-infected and uninfected ticks had similar composition. This study addressed the first report of fungal cytotoxicity analyzing ultrastructural effects on hemocytes of R. microplus infected with entomopathogenic fungi. These results open new perspectives for the comprehension of ticks physiology and pathology, allowing the identification of new targets for the biological control.}, keywords = {Immunity; Cell Death; Fungal infection; Entomopathogenic fungi; tick}, year = {2019}, eissn = {1664-042X} } @article{MTMT:30907275, title = {Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process}, url = {https://m2.mtmt.hu/api/publication/30907275}, author = {Kim-Jo, Chami and Gatti, Jean-Luc and Poirie, Marylene}, doi = {10.3389/fphys.2019.00603}, journal-iso = {FRONT PHYSIOL}, journal = {FRONTIERS IN PHYSIOLOGY}, volume = {10}, unique-id = {30907275}, abstract = {Host-parasitoid interactions are among the most studied interactions between invertebrates because of their fundamental interest - the evolution of original traits in parasitoids - and applied, parasitoids being widely used in biological control. Immunity, and in particular cellular immunity, is central in these interactions, the host encapsulation response being specific for large foreign bodies such as parasitoid eggs. Although already well studied in this species, recent data on Drosophila melanogaster have unquestionably improved knowledge of invertebrate cellular immunity. At the same time, the venomics of parasitoids has expanded, notably those of Drosophila. Here, we summarize and discuss these advances, with a focus on an emerging "time-dependent" view of interactions outcome at the intra- and interspecific level. We also present issues still in debate and prospects for study. Data on the Drosophila-parasitoid model paves the way to new concepts in insect immunity as well as parasitoid wasp strategies to overcome it.}, keywords = {VENOM; DROSOPHILA; Immunity; encapsulation; Hematopoiesis; Parasitoid wasp; Leptopilina}, year = {2019}, eissn = {1664-042X} } @article{MTMT:30901161, title = {Chemical depletion of phagocytic immune cells in Anopheles gambiae reveals dual roles of mosquito hemocytes in anti-Plasmodium immunity}, url = {https://m2.mtmt.hu/api/publication/30901161}, author = {Kwon, Hyeogsun and Smith, Ryan C.}, doi = {10.1073/pnas.1900147116}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {116}, unique-id = {30901161}, issn = {0027-8424}, abstract = {Mosquito immunity is composed of both cellular and humoral factors that provide protection from invading pathogens. Immune cells known as hemocytes, have been intricately associated with phagocytosis and innate immune signaling. However, the lack of genetic tools has limited hemocyte study despite their importance in mosquito anti-Plasmodium immunity. To address these limitations, we employ the use of a chemical-based treatment to deplete phagocytic immune cells in Anopheles gambiae, demonstrating the role of phagocytes in complement recognition and prophenoloxidase production that limit the ookinete and oocyst stages of malaria parasite development, respectively. Through these experiments, we also define specific subtypes of phagocytic immune cells in An. gambiae, providing insights beyond the morphological characteristics that traditionally define mosquito hemocyte populations. Together, this study represents a significant advancement in our understanding of the roles of mosquito phagocytes in mosquito vector competence and demonstrates the utility of clodronate liposomes as an important tool in the study of invertebrate immunity.}, keywords = {HEMOCYTES; innate immunity; Malaria; Anopheles gambiae; phagocyte depletion}, year = {2019}, eissn = {1091-6490}, pages = {14119-14128} } @article{MTMT:30495153, title = {Phagocytosis: what's on the menu?}, url = {https://m2.mtmt.hu/api/publication/30495153}, author = {Lancaster, Charlene E. and Ho, Cheuk Y. and Hipolito, Victoria E. B. and Botelho, Roberto J. and Terebiznik, Mauricio R.}, doi = {10.1139/bcb-2018-0008}, journal-iso = {BIOCHEM CELL BIOL}, journal = {BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE}, volume = {97}, unique-id = {30495153}, issn = {0829-8211}, abstract = {Phagocytosis is an evolutionarily conserved process. In Protozoa, phagocytosis fulfills a feeding mechanism, while in Metazoa, phagocytosis diversified to play multiple organismal roles, including immune defence, tissue homeostasis, and remodeling. Accordingly, phagocytes display a high level of plasticity in their capacity to recognize, engulf, and process targets that differ in composition and morphology. Here, we review how phagocytosis adapts to its multiple roles and discuss in particular the effect of target morphology in phagocytic uptake and phagosome maturation.}, keywords = {PHAGOCYTOSIS; target morphology; phagocytic plasticity; phagosomal maturation; phagocytic cup}, year = {2019}, eissn = {1208-6002}, pages = {21-29} } @article{MTMT:31149703, title = {Independent effects on cellular and humoral immune responses underlie genotype-by-genotype interactions between Drosophila and parasitoids}, url = {https://m2.mtmt.hu/api/publication/31149703}, author = {Leitão, A.B. and Bian, X. and Day, J.P. and Pitton, S. and Demir, E. and Jiggins, F.M.}, doi = {10.1371/journal.ppat.1008084}, journal-iso = {PLOS PATHOG}, journal = {PLOS PATHOGENS}, volume = {15}, unique-id = {31149703}, issn = {1553-7366}, abstract = {It is common to find abundant genetic variation in host resistance and parasite infectivity within populations, with the outcome of infection frequently depending on genotype-specific interactions. Underlying these effects are complex immune defenses that are under the control of both host and parasite genes. We have found extensive variation in Drosophila melanogaster’s immune response against the parasitoid wasp Leptopilina boulardi. Some aspects of the immune response, such as phenoloxidase activity, are predominantly affected by the host genotype. Some, such as upregulation of the complement-like protein Tep1, are controlled by the parasite genotype. Others, like the differentiation of immune cells called lamellocytes, depend on the specific combination of host and parasite genotypes. These observations illustrate how the outcome of infection depends on independent genetic effects on different aspects of host immunity. As parasite-killing results from the concerted action of different components of the immune response, these observations provide a physiological mechanism to generate phenomena like epistasis and genotype-interactions that underlie models of coevolution. © 2019 Leitão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.}, keywords = {Female; Male; GENE; ARTICLE; Cell Differentiation; Genotype; controlled study; nonhuman; cell proliferation; enzyme activity; Blood Cell Count; Gene Expression; Drosophila melanogaster; encapsulation; host resistance; humoral immunity; cellular immunity; blood cell; Parasitoid; WASP; parasite virulence; monophenol monooxygenase; host microbe interaction; Tep1 gene}, year = {2019}, eissn = {1553-7374} } @article{MTMT:31149702, title = {SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila}, url = {https://m2.mtmt.hu/api/publication/31149702}, author = {Paddibhatla, I. and Gautam, D.K. and Mishra, R.K.}, doi = {10.1016/j.ydbio.2019.08.008}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {456}, unique-id = {31149702}, issn = {0012-1606}, abstract = {Proper genetic and epigenetic regulation is necessary to maintain the identity and integrity of cells. Enzymes involved in post-transcriptional modifications of histones are key factors in epigenetic mechanisms. Such modifications are also gaining importance for their role in growth and development of cancer. SETDB1 catalyzes the epigenetic mark of lysine-9 methylation of histone-3. In this study, we explored the role of SETDB1 in Drosophila hematopoiesis. We show that SETDB1 controls the differentiation of matured blood cells in wandering third instar larvae. There are three matured blood cells in wild type Drosophila melanogaster: plasmatocytes, crystal cells and lamellocytes. We found that loss-of-function mutants of SETDB1 show hematopoietic defects; increased blood cell proliferation, decreased number of crystal cells, greater differentiation of blood cells into lamellocytes, dysplasia of the anterior lobes of lymph gland and presence of hematopoietic tumors. Cell type specific knockdown of SETDB1 provided similar phenotype i.e., decreased number of crystal cells and an increase in lamellocyte differentiation. In animals with loss of function of SETDB1, Notch pathway was downregulated. Further, over-expression of SETDB1 in blood cells resulted in an increase in the number of crystal cells. This increase is accompanied with an increase in the number of NotchICD expressing cells. We therefore performed genetic rescue using UAS-GAL4 system to rescue loss of function SETDB1 mutants. Our data show that the rescued larvae carrying a wild type copy of SETDB1 in mutant background are devoid of blood tumors. We have identified a novel dual function of SETDB1 methylatransferase as a critical regulator of two of the matured hemocytes, crystal cells and lamellocytes. We propose a novel role of SETDB1 in modulating the differentiation of crystal cells and lamellocytes from a common progenitor and underscore the importance of SETDB1 in Drosophila blood tumor suppression. © 2019 The Authors}, keywords = {ARTICLE; Cell Differentiation; priority journal; controlled study; nonhuman; cell proliferation; Drosophila melanogaster; unclassified drug; Blood cells; blood cell; plasma cell; hematologic malignancy; loss of function mutation; dysplasia; Hematopoiesis; Hematopoiesis; Notch signaling; Lamellocyte; histone methyltransferase; SETDB1; crystal cells; lamellocytes; lymph glands; crystal cell; Microtumors; SETDB1 protein}, year = {2019}, eissn = {1095-564X}, pages = {74-85} } @article{MTMT:30907595, title = {Simu-dependent clearance of dying cells regulates macrophage function and inflammation resolution}, url = {https://m2.mtmt.hu/api/publication/30907595}, author = {Roddie, Hannah Grace and Armitage, Emma Louise and Coates, Jonathon Alexis and Johnston, Simon Andrew and Evans, Iwan Robert}, doi = {10.1371/journal.pbio.2006741}, journal-iso = {PLOS BIOL}, journal = {PLOS BIOLOGY}, volume = {17}, unique-id = {30907595}, issn = {1544-9173}, abstract = {Macrophages encounter and clear apoptotic cells during normal development and homeostasis, including at numerous sites of pathology. Clearance of apoptotic cells has been intensively studied, but the effects of macrophage-apoptotic cell interactions on macrophage behaviour are poorly understood. Using Drosophila embryos, we have exploited the ease of manipulating cell death and apoptotic cell clearance in this model to identify that the loss of the apoptotic cell clearance receptor Six-microns-under (Simu) leads to perturbation of macrophage migration and inflammatory responses via pathological levels of apoptotic cells. Removal of apoptosis ameliorates these phenotypes, while acute induction of apoptosis phenocopies these defects and reveals that phagocytosis of apoptotic cells is not necessary for their anti-inflammatory action. Furthermore, Simu is necessary for clearance of necrotic debris and retention of macrophages at wounds. Thus, Simu is a general detector of damaged self and represents a novel molecular player regulating macrophages during resolution of inflammation.}, year = {2019}, eissn = {1545-7885} } @article{MTMT:30901163, title = {Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors}, url = {https://m2.mtmt.hu/api/publication/30901163}, author = {Schmid, Martin R. and Dziedziech, Alexis and Arefin, Badrul and Kienzle, Thomas and Wang, Zhi and Akhter, Munira and Berka, Jakub and Theopold, Ulrich}, doi = {10.1016/j.ibmb.2019.04.007}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {109}, unique-id = {30901163}, issn = {0965-1748}, abstract = {In most insects, hemolymph coagulation, which is analogous to mammalian blood clotting, involves close collaboration between humoral and cellular components. To gain insights into the secretion of cellular clotting factors, we created tagged versions of three different clotting factors. Our focus was on factors which are released in a non-classical manner and to characterize them in comparison to a protein that is classically released, namely Glutactin (Glt). Transglutaminase-A (Tg) and Prophenoloxidase 2 (PPO2), both of which lack signal peptide sequences, have been previously demonstrated to be released from plasmatocytes and crystal cells (CCs) respectively, the two hemocyte classes in naive larvae. We found that at the molecular level, Tg secretion resembles the release of tissue transglutaminase in mammals. Specifically, Drosophila Tg is associated with vesicular membranes and remains membrane-bound after release, in contrast to Glt, which we found localizes to a different class of vesicles and is integrated into clot fibers. PPO2 on the other hand, is set free from CCs through cytolysis. We confirm that PPO2 is a central component of the cytosolic crystals and find that the distribution of PPO2 appears to vary across crystals and cells. We propose a tentative scheme for the secretory events during early and late hemolymph coagulation.}, keywords = {TRANSGLUTAMINASE; COAGULATION; SECRETION; BACTERIA; Drosophila melanogaster; Defense; Hemolymph; clotting; Wounding; prophenoloxidase; Non-classical secretion; Glutactin; Septic injury}, year = {2019}, eissn = {1879-0240}, pages = {63-71}, orcid-numbers = {Arefin, Badrul/0000-0003-1117-9125} } @article{MTMT:30510577, title = {A comparative global proteomic analysis of the hematopoietic lineages in the crustacean Pacifastacus leniusculus}, url = {https://m2.mtmt.hu/api/publication/30510577}, author = {Soderhall, Irene and Junkunlo, Kingkamon}, doi = {10.1016/j.dci.2018.11.016}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {92}, unique-id = {30510577}, issn = {0145-305X}, abstract = {In crustaceans as in other arthropods, the circulating hemocytes are vital for protecting the animal against attacking microorganisms. As many hemocytes are destroyed early during an infection, new hemocytes must fast get in place to prevent disperse of a pathogenic microbe, In order to understand the hematopoietic process in more detail we here report a complete proteomic analysis from purified cell types from the APC of the hematopoietic tissue, via the remaining parts of the HPT to the mature semigranular and granular hemocytes. Several possible cell type specific proteins are detected and new putative biomarkers within the crayfish hematopoietic lineage that can be used to increase the understanding of how the differentiation process is regulated is described.}, keywords = {innate immunity; Crustacea; invertebrate; biomarker; proteome; Hematopoiesis}, year = {2019}, eissn = {1879-0089}, pages = {170-178} } @article{MTMT:31149704, title = {The initial stage of tumorigenesis in drosophila epithelial tissues}, url = {https://m2.mtmt.hu/api/publication/31149704}, author = {Tamori, Y.}, doi = {10.1007/978-3-030-23629-8_5}, journal-iso = {ADV EXP MED BIOL}, journal = {ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY}, volume = {1167}, unique-id = {31149704}, issn = {0065-2598}, abstract = {Cancer development originates in a single mutant cell transformed from a normal cell, including further evolution of pro-tumor cells through additional mutations into malignant cancer tissues. Data from recent studies, however, suggest that most pro-tumor cells do not develop into tumors but remain dormant within or are prophylactically eliminated from tissues unless bestowed with additional driver mutations. Drosophila melanogaster has provided very efficient model systems, such as imaginal discs and ovarian follicular epithelia, to study the initial stage of tumorigenesis. This review will focus on the behaviors of emerging pro-tumor cells surrounded by normal cells and situations where they initiate tumor development. © Springer Nature Switzerland AG 2019.}, keywords = {Animals; COMPETITION; animal; nonhuman; epithelium; epithelium; epithelium; pathology; disease course; Drosophila melanogaster; Drosophila melanogaster; human cell; human tissue; cancer staging; CARCINOGENESIS; CARCINOGENESIS; ovary follicle; cancer tissue; tumor cell; Tumorigenesis; cell mutant; Drosophila Proteins; Drosophila protein; imaginal disc; IMAGINAL DISCS; cell competition; epithelial tissues; Tumor hotspot}, year = {2019}, eissn = {2214-8019}, pages = {87-103} } @article{MTMT:30509856, title = {Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/30509856}, author = {Valanne, Susanna and Salminen, Tiina S. and Jarvela-Stolting, Mirva and Vesala, Laura and Ramet, Mika}, doi = {10.1371/journal.ppat.1007504}, journal-iso = {PLOS PATHOG}, journal = {PLOS PATHOGENS}, volume = {15}, unique-id = {30509856}, issn = {1553-7366}, abstract = {Non-coding RNAs have important roles in regulating physiology, including immunity. Here, we performed transcriptome profiling of immune-responsive genes in Drosophila melanogaster during a Gram-positive bacterial infection, concentrating on long non-coding RNA (lncRNA) genes. The gene most highly induced by a Micrococcus luteus infection was CR44404, named Induced by Infection (lincRNA-IBIN). lincRNA-IBIN is induced by both Gram-positive and Gram-negative bacteria in Drosophila adults and parasitoid wasp Leptopilina boulardi in Drosophila larvae, as well as by the activation of the Toll or the Imd pathway in unchallenged flies. We show that upon infection, lincRNA-IBIN is expressed in the fat body, in hemocytes and in the gut, and its expression is regulated by NF-B signaling and the chromatin modeling brahma complex. In the fat body, overexpression of lincRNA-IBIN affected the expression of Toll pathway -mediated genes. Notably, overexpression of lincRNA-IBIN in unchallenged flies elevated sugar levels in the hemolymph by enhancing the expression of genes important for glucose retrieval. These data show that lncRNA genes play a role in Drosophila immunity and indicate that lincRNA-IBIN acts as a link between innate immune responses and metabolism.}, year = {2019}, eissn = {1553-7374} } @article{MTMT:30585796, title = {Headcase is a Repressor of Lamellocyte Fate in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/30585796}, author = {Varga, Gergely István and Csordás, Gábor and Cinege, Gyöngyi Ilona and Jankovics, Ferenc and Sinka, Rita and Kurucz, Judit Éva and Andó, István and Honti, Viktor}, doi = {10.3390/genes10030173}, journal-iso = {GENES-BASEL}, journal = {GENES}, volume = {10}, unique-id = {30585796}, issn = {2073-4425}, abstract = {Due to the evolutionary conservation of the regulation of hematopoiesis, Drosophila provides an excellent model organism to study blood cell differentiation and hematopoietic stem cell (HSC) maintenance. The larvae of Drosophila melanogaster respond to immune induction with the production of special effector blood cells, the lamellocytes, which encapsulate and subsequently kill the invader. Lamellocytes differentiate as a result of a concerted action of all three hematopoietic compartments of the larva: the lymph gland, the circulating hemocytes, and the sessile tissue. Within the lymph gland, the communication of the functional zones, the maintenance of HSC fate, and the differentiation of effector blood cells are regulated by a complex network of signaling pathways. Applying gene conversion, mutational analysis, and a candidate based genetic interaction screen, we investigated the role of Headcase (Hdc), the homolog of the tumor suppressor HECA in the hematopoiesis of Drosophila. We found that naive loss-of-function hdc mutant larvae produce lamellocytes, showing that Hdc has a repressive role in effector blood cell differentiation. We demonstrate that hdc genetically interacts with the Hedgehog and the Decapentaplegic pathways in the hematopoietic niche of the lymph gland. By adding further details to the model of blood cell fate regulation in the lymph gland of the larva, our findings contribute to the better understanding of HSC maintenance.}, keywords = {DIFFERENTIATION; DROSOPHILA; innate immunity; blood cell; niche; Hematopoiesis; Hemocyte}, year = {2019}, eissn = {2073-4425}, orcid-numbers = {Varga, Gergely István/0000-0001-9073-5788; Csordás, Gábor/0000-0001-6871-6839; Sinka, Rita/0000-0003-4040-4184; Andó, István/0000-0002-4648-9396} } @article{MTMT:30510582, title = {Hedgehog signaling from the Posterior Signaling Center maintains U-shaped expression and a prohemocyte population in Drosophila}, url = {https://m2.mtmt.hu/api/publication/30510582}, author = {Baldeosingh, Rajkumar and Gao, Hongjuan and Wu, Xiaorong and Fossett, Nancy}, doi = {10.1016/j.ydbio.2018.06.020}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {441}, unique-id = {30510582}, issn = {0012-1606}, abstract = {Hematopoietic progenitor choice between multipotency and differentiation is tightly regulated by intrinsic factors and extrinsic signals from the surrounding microenvironment. The Drosophila melanogaster hematopoietic lymph gland has emerged as a powerful tool to investigate mechanisms that regulate hematopoietic progenitor choice in vivo. The lymph gland contains progenitor cells, which share key characteristics with mammalian hematopoietic progenitors such as quiescence, multipotency and niche dependence. The lymph gland is zonally arranged, with progenitors located in medullary zone, differentiating cells in the cortical zone, and the stem cell niche or Posterior Signaling Center (PSC) residing at the base of the medullary zone (MZ). This arrangement facilitates investigations into how signaling from the microenvironment controls progenitor choice. The Drosophila Friend of GATA transcriptional regulator, U-shaped, is a conserved hematopoietic regulator. To identify additional novel intrinsic and extrinsic regulators that interface with U-shaped to control hematopoiesis, we conducted an in vivo screen for factors that genetically interact with u-shaped. Smoothened, a downstream effector of Hedgehog signaling, was one of the factors identified in the screen. Here we report our studies that characterized the relationship between Smoothened and U-shaped. We showed that the PSC and Hedgehog signaling are required for U-shaped expression and that U-shaped is an important intrinsic progenitor regulator. These observations identify a potential link between the progenitor regulatory machinery and extrinsic signals from the PSC. Furthermore, we showed that both Hedgehog signaling and the PSC are required to maintain a subpopulation of progenitors. This led to a delineation of PSC-dependent versus PSC-independent progenitors and provided further evidence that the MZ progenitor population is heterogeneous. Overall, we have identified a connection between a conserved hematopoietic master regulator and a putative stem cell niche, which adds to our understanding of how signals from the microenvironment regulate progenitor multipotency.}, year = {2018}, eissn = {1095-564X}, pages = {132-145} } @article{MTMT:30510499, title = {From Drosophila Blood Cells to Human Leukemia}, url = {https://m2.mtmt.hu/api/publication/30510499}, author = {Boulet, Manon and Miller, Marion and Vandel, Laurence and Waltzer, Lucas}, doi = {10.1007/978-981-13-0529-0_11}, journal-iso = {ADV EXP MED BIOL}, journal = {ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY}, volume = {1076}, unique-id = {30510499}, issn = {0065-2598}, abstract = {The hematopoietic system plays a critical role in establishing the proper response against invading pathogens or in removing cancerous cells. Furthermore, deregulations of the hematopoietic differentiation program are at the origin of numerous diseases including leukemia. Importantly, many aspects of blood cell development have been conserved from human to Drosophila. Hence, Drosophila has emerged as a potent genetic model to study blood cell development and leukemia in vivo. In this chapter, we give a brief overview of the Drosophila hematopoietic system, and we provide a protocol for the dissection and the immunostaining of the larval lymph gland, the most studied hematopoietic organ in Drosophila. We then focus on the various paradigms that have been used in fly to investigate how conserved genes implicated in leukemogenesis control blood cell development. Specific examples of Drosophila models for leukemia are presented, with particular attention to the most translational ones. Finally, we discuss some limitations and potential improvements of Drosophila models for studying blood cell cancer.}, keywords = {LEUKEMIA; DROSOPHILA; SCREEN; Hematopoiesis}, year = {2018}, eissn = {2214-8019}, pages = {195-214} } @article{MTMT:27523475, title = {CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera}, url = {https://m2.mtmt.hu/api/publication/27523475}, author = {Cheng, Yang and Lin, Zhe and Wang, Ju-Mei and Xing, Long-Sheng and Xiong, Guang-Hua and Zou, Zhen}, doi = {10.1016/j.dci.2018.02.010}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {84}, unique-id = {27523475}, issn = {0145-305X}, year = {2018}, eissn = {1879-0089}, pages = {142-152} } @article{MTMT:31149706, title = {The interplay between immunity and aging in Drosophila}, url = {https://m2.mtmt.hu/api/publication/31149706}, author = {Flatt, T. and Garschall, K.}, doi = {10.12688/f1000research.13117.1}, journal-iso = {F1000RESEARCH}, journal = {F1000RESEARCH}, volume = {7}, unique-id = {31149706}, issn = {2046-1402}, abstract = {Here, we provide a brief review of the mechanistic connections between immunity and aging-a fundamental biological relationship that remains poorly understood-by considering two intertwined questions: how does aging affect immunity, and how does immunity affect aging? On the one hand, aging contributes to the deterioration of immune function and predisposes the organism to infections ("immuno-senescence"). On the other hand, excessive activation of the immune system can accelerate degenerative processes, cause inflammation and immunopathology, and thus promote aging ("inflammaging"). Interestingly, several recent lines of evidence support the hypothesis that restrained or curbed immune activity at old age (that is, optimized age-dependent immune homeostasis) might actually improve realized immune function and thereby promote longevity. We focus mainly on insights from Drosophila, a powerful genetic model system in which both immunity and aging have been extensively studied, and conclude by outlining several unresolved questions in the field. © 2018 Garschall K and Flatt T.}, keywords = {Inflammation; GENE; metabolism; DEGENERATION; INFECTION; EVOLUTION; signal transduction; LONGEVITY; LONGEVITY; DROSOPHILA; DROSOPHILA; toll like receptor; review; disease predisposition; nonhuman; Immunity; Immunity; Aging; Aging; immune response; immunopathology; upregulation; lifespan; immunoglobulin enhancer binding protein; evidence based medicine; deterioration; cellular stress response; immunosenescence; immunosenescence; genetic model; microbiome; inflammaging}, year = {2018} } @article{MTMT:27523477, title = {Innate immune memory: An evolutionary perspective}, url = {https://m2.mtmt.hu/api/publication/27523477}, author = {Gourbal, Benjamin and Pinaud, Silvain and Beckers, Gerold J M and Van, Der Meer Jos W M and Conrath, Uwe and Netea, Mihai G}, doi = {10.1111/imr.12647}, journal-iso = {IMMUNOL REV}, journal = {IMMUNOLOGICAL REVIEWS}, volume = {283}, unique-id = {27523477}, issn = {0105-2896}, year = {2018}, eissn = {1600-065X}, pages = {21-40}, orcid-numbers = {Conrath, Uwe/0000-0002-8503-1943} } @article{MTMT:27523474, title = {Characterisation and functional comparison of single-CRD and multidomain containing galectins CgGal-2 and CgGal-3 from oyster Crassostrea gigas}, url = {https://m2.mtmt.hu/api/publication/27523474}, author = {Huang, Mengmeng and Zhou, Tao and Wu, Yuehong and Fei, Hui and Wang, Gaoyang and Li, Zhi and Lei, Yutong and Liu, Qian and Sun, Cong and Lv, Zhengbing and Xu, Xue-Wei}, doi = {10.1016/j.fsi.2018.04.029}, journal-iso = {FISH SHELLFISH IMMUN}, journal = {FISH AND SHELLFISH IMMUNOLOGY}, volume = {78}, unique-id = {27523474}, issn = {1050-4648}, year = {2018}, eissn = {1095-9947}, pages = {238-247} } @article{MTMT:30514422, title = {Time-course analysis of Drosophila suzukii interaction with endoparasitoid wasps evidences a delayed encapsulation response compared to D-melanogaster}, url = {https://m2.mtmt.hu/api/publication/30514422}, author = {Iacovone, Alessia and Ris, Nicolas and Poirie, Marylene and Gatti, Jean-Luc}, doi = {10.1371/journal.pone.0201573}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {13}, unique-id = {30514422}, issn = {1932-6203}, abstract = {Drosophila suzukii (the spotted-wing Drosophila) appears to be unsuitable for the development of most Drosophila larval endoparasitoids, be they sympatric or not. Here, we questioned the physiological bases of this widespread failure by characterizing the interactions between D. suzukii and various parasitoid species (Asobara japonica, Leptopiiina boulardi, Leptopilina heterotoma and Leptopilina victoriae) and comparing them with those observed with D. meianogaster, a rather appropriate host. All parasitoids were able to oviposit in L1 and L2 larval stages of both hosts but their propensity to parasitize was higher on D. meianogaster. A. japonica and, to a much lesser extent, L. heterotoma, were the two species able to successfully develop in D. suzukii, the failure of the parasitism resulting either in the parasitoid encapsulation (notably with L. heterotoma) or the host and parasitoid deaths (especially with L. boulardi and L. victoriae). Compared to D. meianogaster, encapsulation in D. suzukii was strongly delayed and led, if successful, to the production of much larger capsules in surviving flies and, in the event of failure, to the death of both partners because of an uncontrolled melanization. The results thus revealed a different timing of the immune response to parasitoids in D. suzukii compared to D. melanogaster with a lose-lose outcome for parasitoids (generally unsuccessful development) and hosts (high mortality and possible reduction of the fitness of survivors). Finally, these results might suggest that some European endoparasitoids of Drosophila interact with this pest in the field in an unmeasurable way, since they kill their host without reproductive success.}, year = {2018}, eissn = {1932-6203} } @article{MTMT:31149601, title = {Detecting proliferation of adult hemocytes in Drosophila by BrdU incorporation [version 1; referees: 2 approved]}, url = {https://m2.mtmt.hu/api/publication/31149601}, author = {Mandal, L. and Ghosh, S. and Mandal, S.}, doi = {10.12688/wellcomeopenres.14560.1}, journal-iso = {WELLCOME OPEN RESEARCH}, journal = {WELLCOME OPEN RESEARCH}, volume = {3}, unique-id = {31149601}, issn = {2398-502X}, abstract = {Drosophila and mammalian hematopoiesis share several similarities that ranges from phases to the battery of transcription factors and signaling molecules that execute this process. These resounding similarities along with the rich genetic tools available in fruitfly makes it a popular invertebrate model to study blood cell development both during normal and aberrant conditions. The larval system is the most extensively studied to date. Several studies have shown that these hemocytes just like mammalian counterpart proliferate and get routinely regenerated upon infection. However, employing the same protocol it was concluded that blood cell proliferation although abundant in larval stages is absent in adult fruitfly. The current protocol describes the strategies that can be employed to document the hemocyte proliferation in adulthood. The fact that a subset of blood cells tucked away in the hematopoietic hub are not locked in senescence, rather they still harbour the proliferative capacity to tide over challenges was successfully demonstrated by this method. Although we have adopted bacterial infection as a bait to evoke this proliferative capacity of the hemocytes, we envision that it can also efficiently characterize the proliferative responses of hemocytes in tumorigenic conditions as well as scenarios of environmental and metabolic stresses during adulthood. © 2018 Ghosh S et al.}, keywords = {INFECTION; PROLIFERATION; MACROPHAGE; Hematopoiesis; Adult Drosophila}, year = {2018} } @article{MTMT:27524971, title = {DNA extracellular traps are part of the immune repertoire of Periplaneta americana}, url = {https://m2.mtmt.hu/api/publication/27524971}, author = {Nascimento, M T C and Silva, K P and Garcia, M C F and Medeiros, M N and Machado, E A and Nascimento, S B and Saraiva, E M}, doi = {10.1016/j.dci.2018.01.012}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {84}, unique-id = {27524971}, issn = {0145-305X}, year = {2018}, eissn = {1879-0089}, pages = {62-70} } @article{MTMT:30514419, title = {An in vitro method for studying subcellular rearrangements during cell polarization in Drosophila melanogaster hemocytes}, url = {https://m2.mtmt.hu/api/publication/30514419}, author = {Sofia Edwards, Sandra and Delgado, Maria Graciela and Nader, Guilherme Pedreira de Freitas and Piel, Matthieu and Bellaiche, Yohanns and Lennon-Dumenil, Ana Maria and Glavic, Alvaro}, doi = {10.1016/j.mod.2018.08.003}, journal-iso = {MECH DEVELOP}, journal = {MECHANISMS OF DEVELOPMENT}, volume = {154}, unique-id = {30514419}, issn = {0925-4773}, abstract = {Thanks to the power of Drosophila genetics, this animal model has been a precious tool for scientists to uncover key processes associated to innate immunity. The fly immune system relies on a population of macrophage-like cells, also referred to as hemocytes, which are highly migratory and phagocytic, and can easily be followed in vivo. These cells have shown to play important roles in fly development, both at the embryonic and pupal stages. However, there is no robust assay for the study of hemocyte migration in vitro, which limits our understanding of the molecular mechanisms involved. Here, we contribute to fill this gap by showing that hemocytes adopt a polarized morphology upon ecdysone stimulation, allowing the study of the cytoskeleton rearrangements and organelle reorganization that take place during the first step of cell locomotion.}, keywords = {DROSOPHILA; HEMOCYTES; MIGRATION; In Vitro; POLARIZATION; PROTOCOL; ecdysone}, year = {2018}, eissn = {1872-6356}, pages = {277-286} } @article{MTMT:27048470, title = {Proprotein convertase Furin1 expression in the Drosophila fat body is essential for a normal antimicrobial peptide response and bacterial host defense}, url = {https://m2.mtmt.hu/api/publication/27048470}, author = {Aittomaki, Saara and Valanne, Susanna and Lehtinen, Tapio and Matikainen, Sampsa and Nyman, Tuula A and Ramet, Mika and Pesu, Marko}, doi = {10.1096/fj.201700296R}, journal-iso = {FASEB J}, journal = {FASEB JOURNAL}, volume = {31}, unique-id = {27048470}, issn = {0892-6638}, year = {2017}, eissn = {1530-6860}, pages = {4770-4782} } @article{MTMT:26891561, title = {A Genetic Screen Reveals an Unexpected Role for Yorkie Signaling in JAK/STAT-Dependent Hematopoietic Malignancies in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/26891561}, author = {Anderson, Abigail M and Bailetti, Alessandro A and Rodkin, Elizabeth and De, Atish and Bach, Erika A}, doi = {10.1534/g3.117.044172}, journal-iso = {G3-GENES GENOM GENET}, journal = {G3-GENES GENOMES GENETICS}, volume = {7}, unique-id = {26891561}, issn = {2160-1836}, year = {2017}, eissn = {2160-1836}, pages = {2427-2438} } @article{MTMT:26714387, title = {The Immune Phenotype of Three Drosophila Leukemia Models}, url = {https://m2.mtmt.hu/api/publication/26714387}, author = {Arefin, Badrul and Kunc, Martin and Krautz, Robert and Theopold, Ulrich}, doi = {10.1534/g3.117.039487}, journal-iso = {G3-GENES GENOM GENET}, journal = {G3-GENES GENOMES GENETICS}, volume = {7}, unique-id = {26714387}, issn = {2160-1836}, year = {2017}, eissn = {2160-1836}, pages = {2139-2149} } @article{MTMT:27049410, title = {Comparative analysis of gene expression profiles for several migrating cell types identifies cell migration regulators}, url = {https://m2.mtmt.hu/api/publication/27049410}, author = {Bae, Young-Kyung and Macabenta, Frank and Curtis, Heather Leigh and Stathopoulos, Angelike}, doi = {10.1016/j.mod.2017.04.004}, journal-iso = {MECH DEVELOP}, journal = {MECHANISMS OF DEVELOPMENT}, volume = {148}, unique-id = {27049410}, issn = {0925-4773}, year = {2017}, eissn = {1872-6356}, pages = {40-55} } @article{MTMT:26377430, title = {Human NUP98-HOXA9 promotes hyperplastic growth of hematopoietic tissues in Drosophila}, url = {https://m2.mtmt.hu/api/publication/26377430}, author = {Baril, Caroline and Gavory, Gwenaelle and Bidla, Gawa and Knaevelsrud, Helene and Sauvageau, Guy and Therrien, Marc}, doi = {10.1016/j.ydbio.2016.11.003}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {421}, unique-id = {26377430}, issn = {0012-1606}, year = {2017}, eissn = {1095-564X}, pages = {16-26} } @article{MTMT:26536119, title = {Symbiont-induced odorant binding proteins mediate insect host hematopoiesis}, url = {https://m2.mtmt.hu/api/publication/26536119}, author = {Benoit, Joshua B and Vigneron, Aurelien and Broderick, Nichole A and Wu, Yineng and Sun, Jennifer S and Carlson, John R and Aksoy, Serap and Weiss, Brian L}, doi = {10.7554/eLife.19535}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {6}, unique-id = {26536119}, issn = {2050-084X}, year = {2017}, eissn = {2050-084X}, orcid-numbers = {Aksoy, Serap/0000-0001-9941-143X} } @article{MTMT:26715309, title = {Drosophila as a Model for Human Diseases-Focus on Innate Immunity in Barrier Epithelia}, url = {https://m2.mtmt.hu/api/publication/26715309}, author = {Bergman, P and Esfahani, S Seyedoleslami and Engstrom, Y}, doi = {10.1016/bs.ctdb.2016.07.002}, editor = {Pick, L}, journal-iso = {CURR TOP DEV BIOL}, journal = {CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY}, volume = {121}, unique-id = {26715309}, issn = {0070-2153}, year = {2017}, eissn = {1557-8933}, pages = {29-81} } @article{MTMT:27055182, title = {dOCRL maintains immune cell quiescence by regulating endosomal traffic}, url = {https://m2.mtmt.hu/api/publication/27055182}, author = {Del Signore, Steven J and Biber, Sarah A and Lehmann, Katherine S and Heimler, Stephanie R and Rosenfeld, Benjamin H and Eskin, Tania L and Sweeney, Sean T and Rodal, Avital A}, doi = {10.1371/journal.pgen.1007052}, journal-iso = {PLOS GENET}, journal = {PLOS GENETICS}, volume = {13}, unique-id = {27055182}, issn = {1553-7390}, year = {2017}, eissn = {1553-7404} } @article{MTMT:26891459, title = {Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps}, url = {https://m2.mtmt.hu/api/publication/26891459}, author = {Dostalova, Anna and Rommelaere, Samuel and Poidevin, Mickael and Lemaitre, Bruno}, doi = {10.1186/s12915-017-0408-0}, journal-iso = {BMC BIOL}, journal = {BMC BIOLOGY}, volume = {15}, unique-id = {26891459}, issn = {1741-7007}, year = {2017}, eissn = {1741-7007}, orcid-numbers = {Lemaitre, Bruno/0000-0001-7970-1667} } @article{MTMT:26988892, title = {Insect immunity to entomopathogenic nematodes and their mutualistic bacteria}, url = {https://m2.mtmt.hu/api/publication/26988892}, author = {Eleftherianos, I and Shokal, U and Yadav, S and Kenney, E and Maldonado, T}, doi = {10.1007/82_2016_52}, journal-iso = {CURR TOP MICROBIOL}, journal = {CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY}, volume = {402}, unique-id = {26988892}, issn = {0070-217X}, year = {2017}, eissn = {2196-9965}, pages = {123-156} } @article{MTMT:26536855, title = {A novel mode of induction of the humoral innate immune response in Drosophila larvae}, url = {https://m2.mtmt.hu/api/publication/26536855}, author = {Kenmoku, Hiroyuki and Hori, Aki and Kuraishi, Takayuki and Kurata, Shoichiro}, doi = {10.1242/dmm.027102}, journal-iso = {DIS MODEL MECH}, journal = {DISEASE MODELS & MECHANISMS}, volume = {10}, unique-id = {26536855}, issn = {1754-8403}, year = {2017}, eissn = {1754-8411}, pages = {271-281} } @article{MTMT:26891460, title = {Modulation of occluding junctions alters the hematopoietic niche to trigger immune activation}, url = {https://m2.mtmt.hu/api/publication/26891460}, author = {Khadilkar, Rohan J and Vogl, Wayne and Goodwin, Katharine and Tanentzapf, Guy}, doi = {10.7554/eLife.28081}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {6}, unique-id = {26891460}, issn = {2050-084X}, year = {2017}, eissn = {2050-084X} } @article{MTMT:26898817, title = {Anopheles gambiae larvae mount stronger immune responses against bacterial infection than adults: evidence of adaptive decoupling in mosquitoes}, url = {https://m2.mtmt.hu/api/publication/26898817}, author = {League, Garrett P and Estevez-Lao, Tania Y and Yan, Yan and Garcia-Lopez, Valeria A and Hillyer, Julian F}, doi = {10.1186/s13071-017-2302-6}, journal-iso = {PARASITE VECT}, journal = {PARASITES AND VECTORS}, volume = {10}, unique-id = {26898817}, issn = {1756-3305}, year = {2017}, eissn = {1756-3305} } @article{MTMT:27049321, title = {Parallel and costly changes to cellular immunity underlie the evolution of parasitoid resistance in three Drosophila species}, url = {https://m2.mtmt.hu/api/publication/27049321}, author = {McGonigle, John E and Leitao, Alexandre B and Ommeslag, Sarah and Smith, Sophie and Day, Jonathan P and Jiggins, Francis M}, doi = {10.1371/journal.ppat.1006683}, journal-iso = {PLOS PATHOG}, journal = {PLOS PATHOGENS}, volume = {13}, unique-id = {27049321}, issn = {1553-7366}, year = {2017}, eissn = {1553-7374} } @inbook{MTMT:27048320, title = {Phagocytosis in Insect Immunity}, url = {https://m2.mtmt.hu/api/publication/27048320}, author = {Nazario-Toole, Ashley E and Wu, Louisa P}, booktitle = {Advances in Insect Physiology}, doi = {10.1016/bs.aiip.2016.12.001}, unique-id = {27048320}, year = {2017}, pages = {35-82} } @article{MTMT:26698807, title = {The nociception genes painless and Piezo are required for the cellular immune response of Drosophila larvae to wasp parasitization}, url = {https://m2.mtmt.hu/api/publication/26698807}, author = {Tokusumi, Yumiko and Tokusumi, Tsuyoshi and Schulz, Robert A}, doi = {10.1016/j.bbrc.2017.03.116}, journal-iso = {BIOCHEM BIOPH RES CO}, journal = {BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS}, volume = {486}, unique-id = {26698807}, issn = {0006-291X}, year = {2017}, eissn = {1090-2104}, pages = {893-897} } @{MTMT:31149707, title = {Role of the Microbiota During Development of the Arthropod Vector Immune System}, url = {https://m2.mtmt.hu/api/publication/31149707}, author = {Vigneron, A. and Weiss, B.L.}, booktitle = {Arthropod Vector: Controller of Disease Transmission}, doi = {10.1016/B978-0-12-805350-8.00009-X}, volume = {1}, unique-id = {31149707}, abstract = {Arthropod disease vectors and the bacterial microbes they carry exhibit varying levels of coevolutionary integration, and these associations reflect distinct host-microbe physiological codependences. The mosquito microbiota, which is largely transient and environmentally acquired during larval and adult stages, facilitates host maturation and modulates vector competency during adulthood. Unlike mosquitoes, tsetse flies are obligate blood feeders and thus house a steadfast population of vertically transmitted obligate, commensal, and parasitic bacterial symbionts. Tsetse that undergo development in the absence of their indigenous microbiota are severely immunocompromised during adulthood. Specifically, these "aposymbiotic" flies lack a functional cellular immune system and fail to produce a structurally robust peritrophic matrix midgut barrier. Thus, aposymbiotic tsetse adults are unusually susceptible to systemic infection with normally nonpathogenic Escherichia coli and midgut infection with parasitic trypanosomes. Further studies related to deciphering molecular mechanisms that underlie microbiota-modulated maturation of the arthropod disease vector immune system can lead to the development of novel pathogen transmission blocking control strategies. © 2017 Elsevier Inc. All rights reserved.}, keywords = {immune system; Microbiota; host-symbiont interaction; Arthropod disease vector}, year = {2017}, pages = {161-172} }