TY - JOUR AU - Liegeois, S. AU - Ferrandon, D. TI - Sensing microbial infections in the Drosophila melanogaster genetic model organism JF - IMMUNOGENETICS J2 - IMMUNOGENETICS VL - 74 PY - 2022 IS - 1 SP - 35 EP - 62 PG - 28 SN - 0093-7711 DO - 10.1007/s00251-021-01239-0 UR - https://m2.mtmt.hu/api/publication/32898786 ID - 32898786 N1 - Cited By :1 Export Date: 22 June 2022 CODEN: IMNGB AB - Insects occupy a central position in the biosphere. They are able to resist infections even though they lack an adaptive immune system. Drosophila melanogaster has been used as a potent genetic model to understand innate immunity both in invertebrates and vertebrates. Its immune system includes both humoral and cellular arms. Here, we review how the distinct immune responses are triggered upon sensing infections, with an emphasis on the mechanisms that lead to systemic humoral immune responses. As in plants, the components of the cell wall of microorganisms are detected by dedicated receptors. There is also an induction of the systemic immune response upon sensing the proteolytic activities of microbial virulence factors. The antiviral response mostly relies on sensing double-stranded RNAs generated during the viral infection cycle. This event subsequently triggers either the viral short interfering RNA pathway or a cGAS-like/STING/NF-κB signaling pathway. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. LA - English DB - MTMT ER - TY - JOUR AU - Eleftherianos, Ioannis AU - Heryanto, Christa AU - Bassal, Taha AU - Zhang, Wei AU - Tettamanti, Gianluca AU - Mohamed, Amr TI - Haemocyte-mediated immunity in insects: Cells, processes and associated components in the fight against pathogens and parasites JF - IMMUNOLOGY J2 - IMMUNOLOGY VL - 164 PY - 2021 IS - 3 SP - 401 EP - 432 PG - 32 SN - 0019-2805 DO - 10.1111/imm.13390 UR - https://m2.mtmt.hu/api/publication/32361576 ID - 32361576 N1 - Infection and Innate Immunity Laboratory, Department of Biological Sciences, Institute for Biomedical Sciences, The George Washington University, Washington, DC, United States Department of Entomology, Faculty of Science, Cairo University, Giza, Egypt State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Napoli Federico II, Napoli, Italy Cited By :4 Export Date: 19 January 2022 CODEN: IMMUA Correspondence Address: Eleftherianos, I.; Infection and Innate Immunity Laboratory, United States; email: ioannise@gwu.edu Correspondence Address: Mohamed, A.; Department of Entomology, Egypt; email: mamr@sci.cu.edu.eg AB - The host defence of insects includes a combination of cellular and humoral responses. The cellular arm of the insect innate immune system includes mechanisms that are directly mediated by haemocytes (e.g., phagocytosis, nodulation and encapsulation). In addition, melanization accompanying coagulation, clot formation and wound healing, nodulation and encapsulation processes leads to the formation of cytotoxic redox-cycling melanin precursors and reactive oxygen and nitrogen species. However, demarcation between cellular and humoral immune reactions as two distinct categories is not straightforward. This is because many humoral factors affect haemocyte functions and haemocytes themselves are an important source of many humoral molecules. There is also a considerable overlap between cellular and humoral immune functions that span from recognition of foreign intruders to clot formation. Here, we review these immune reactions starting with the cellular mechanisms that limit haemolymph loss and participate in wound healing and clot formation and advancing to cellular functions that are critical in restricting pathogen movement and replication. This information is important because it highlights that insect cellular immunity is controlled by a multilayered system, different components of which are activated by different pathogens or during the different stages of the infection. LA - English DB - MTMT ER - TY - JOUR AU - Gul, Isma AU - Kausar, Saima AU - You, Qiuxiang AU - Sun, Wei AU - Li, Zekun AU - Abbas, Muhammad Nadeem AU - Cui, Hongjuan TI - Identification and the immunological role of two Nimrod family genes in the silkworm, Bombyx mori JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 193 PY - 2021 SP - 154 EP - 165 PG - 12 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2021.10.083 UR - https://m2.mtmt.hu/api/publication/32585067 ID - 32585067 N1 - Export Date: 3 August 2022 LA - English DB - MTMT ER - TY - JOUR AU - Schlamp, F. AU - Delbare, S.Y.N. AU - Early, A.M. AU - Wells, M.T. AU - Basu, S. AU - Clark, A.G. TI - Dense time-course gene expression profiling of the Drosophila melanogaster innate immune response JF - BMC GENOMICS J2 - BMC GENOMICS VL - 22 PY - 2021 IS - 1 SN - 1471-2164 DO - 10.1186/s12864-021-07593-3 UR - https://m2.mtmt.hu/api/publication/32006366 ID - 32006366 N1 - Molecular Biology and Genetics, Cornell University, Ithaca, NY, United States Statistics and Data Science, Cornell University, Ithaca, NY, United States Export Date: 10 May 2021 CODEN: BGMEE Correspondence Address: Schlamp, F.; Molecular Biology and Genetics, United States; email: mfs97@cornell.edu Correspondence Address: Clark, A.G.; Molecular Biology and Genetics, United States; email: ac347@cornell.edu Correspondence Address: Basu, S.; Statistics and Data Science, United States; email: sb2457@cornell.edu AB - Background: Immune responses need to be initiated rapidly, and maintained as needed, to prevent establishment and growth of infections. At the same time, resources need to be balanced with other physiological processes. On the level of transcription, studies have shown that this balancing act is reflected in tight control of the initiation kinetics and shutdown dynamics of specific immune genes. Results: To investigate genome-wide expression dynamics and trade-offs after infection at a high temporal resolution, we performed an RNA-seq time course on D. melanogaster with 20 time points post Imd stimulation. A combination of methods, including spline fitting, cluster analysis, and Granger causality inference, allowed detailed dissection of expression profiles, lead-lag interactions, and functional annotation of genes through guilt-by-association. We identified Imd-responsive genes and co-expressed, less well characterized genes, with an immediate-early response and sustained up-regulation up to 5 days after stimulation. In contrast, stress response and Toll-responsive genes, among which were Bomanins, demonstrated early and transient responses. We further observed a strong trade-off with metabolic genes, which strikingly recovered to pre-infection levels before the immune response was fully resolved. Conclusions: This high-dimensional dataset enabled the comprehensive study of immune response dynamics through the parallel application of multiple temporal data analysis methods. The well annotated data set should also serve as a useful resource for further investigation of the D. melanogaster innate immune response, and for the development of methods for analysis of a post-stress transcriptional response time-series at whole-genome scale. © 2021, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Melcarne, C. AU - Lemaitre, B. AU - Kurant, E. TI - Phagocytosis in Drosophila: From molecules and cellular machinery to physiology JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 109 PY - 2019 SP - 1 EP - 12 PG - 12 SN - 0965-1748 DO - 10.1016/j.ibmb.2019.04.002 UR - https://m2.mtmt.hu/api/publication/30901164 ID - 30901164 N1 - Global Health Institute, School of Life Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland Department of Human Biology, Faculty of Natural Sciences, University of Haifa, Haifa, 34988, Israel Cited By :25 Export Date: 19 January 2022 CODEN: IBMBE Correspondence Address: Lemaitre, B.; Global Health Institute, Switzerland; email: bruno.lemaitre@epfl.ch AB - Phagocytosis is an evolutionarily conserved mechanism that plays a key role in both host defence and tissue homeostasis in multicellular organisms. A range of surface receptors expressed on different cell types allow discriminating between self and non-self (or altered) material, thus enabling phagocytosis of pathogens and apoptotic cells. The phagocytosis process can be divided into four main steps: 1) binding of the phagocyte to the target particle, 2) particle internalization and phagosome formation, through remodelling of the plasma membrane, 3) phagosome maturation, and 4) particle destruction in the phagolysosome. In this review, we describe our present knowledge on phagocytosis in the fruit fly Drosophila melanogaster, assessing each of the key steps involved in engulfment of both apoptotic cells and bacteria. We also assess the physiological role of phagocytosis in host defence, development and tissue homeostasis. LA - English DB - MTMT ER - TY - JOUR AU - Melcarne, Claudia AU - Ramond, Elodie AU - Dudzic, Jan AU - Bretscher, Andrew AU - Kurucz, Judit Éva AU - Andó, István AU - Lemaitre, Bruno TI - Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster JF - FEBS JOURNAL J2 - FEBS J VL - 286 PY - 2019 IS - 14 SP - 2670 EP - 2691 PG - 22 SN - 1742-464X DO - 10.1111/febs.14857 UR - https://m2.mtmt.hu/api/publication/30641961 ID - 30641961 N1 - Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary Cited By :13 Export Date: 19 January 2022 CODEN: FJEOA Correspondence Address: Melcarne, C.; Global Health Institute, Switzerland; email: claudia.melcarne@epfl.ch LA - English DB - MTMT ER - TY - JOUR AU - Sigle, L T AU - Hillyer, J F TI - Eater and draper are involved in the periostial haemocyte immune response in the mosquito Anopheles gambiae JF - INSECT MOLECULAR BIOLOGY J2 - INSECT MOL BIOL VL - 27 PY - 2018 IS - 4 SP - 429 EP - 438 PG - 10 SN - 0962-1075 DO - 10.1111/imb.12383 UR - https://m2.mtmt.hu/api/publication/27602651 ID - 27602651 N1 - Cited By :7 Export Date: 19 January 2022 CODEN: IMBIE Correspondence Address: Hillyer, J.F.; Department of Biological Sciences, United States; email: julian.hillyer@vanderbilt.edu LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Zsámboki, János AU - Vidal-Quadras, M AU - Uv, A AU - Csordás, Gábor AU - Honti, Viktor AU - Gábor, Erika AU - Hegedűs, Zoltán AU - Varga, Gergely István AU - Kovács, Attila Lajos AU - Juhász, Gábor AU - Williams, MJ AU - Andó, István AU - Kurucz, Judit Éva TI - Genes encoding cuticular proteins are components of the Nimrod gene cluster in Drosophila. JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 87 PY - 2017 SP - 45 EP - 54 PG - 10 SN - 0965-1748 DO - 10.1016/j.ibmb.2017.06.006 UR - https://m2.mtmt.hu/api/publication/3249679 ID - 3249679 N1 - Immunology Unit, Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, P.O.Box 521, Szeged, H-6701, Hungary Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SE-40530, Sweden Laboratory of Bioinformatics, Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, P.O.Box 521, Szeged, H-6701, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Department of Neuroscience, Functional Pharmacology, Uppsala University, Sweden Institution För Neurovetenskap BMC, Husargatan 3, Box 593 751 24, Uppsala, Sweden Cited By :10 Export Date: 7 November 2023 CODEN: IBMBE Correspondence Address: Kurucz, É.; Immunology Unit, P.O.Box 521, Hungary; email: kurucz.eva@brc.mta.hu AB - The Nimrod gene cluster, located on the second chromosome of Drosophila melanogaster, is the largest synthenic unit of the Drosophila genome. Nimrod genes show blood cell specific expression and code for phagocytosis receptors that play a major role in fruit fly innate immune functions. We previously identified three homologous genes (vajk-1, vajk-2 and vajk-3) located within the Nimrod cluster, which are unrelated to the Nimrod genes, but are homologous to a fourth gene (vajk-4) located outside the cluster. Here we show that, unlike the Nimrod candidates, the Vajk proteins are expressed in cuticular structures of the late embryo and the late pupa, indicating that they contribute to cuticular barrier functions. LA - English DB - MTMT ER - TY - JOUR AU - Hillyer, Julian F TI - Insect immunology and hematopoiesis JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 58 PY - 2016 SP - 102 EP - 118 PG - 17 SN - 0145-305X DO - 10.1016/j.dci.2015.12.006 UR - https://m2.mtmt.hu/api/publication/25771630 ID - 25771630 N1 - Cited By :187 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Hillyer, J.F.; Department of Biological Sciences, VU Station B 35-1634, United States; email: julian.hillyer@vanderbilt.edu LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Andó, István TI - Innate immunity JF - ACTA BIOLOGICA SZEGEDIENSIS J2 - ACTA BIOL SZEGED VL - 59 PY - 2015 IS - Suppl. 1 SP - 1 EP - 15 PG - 15 SN - 1588-385X UR - https://m2.mtmt.hu/api/publication/2993019 ID - 2993019 N1 - Export Date: 19 January 2022 CODEN: ABSCC Correspondence Address: Andó, I.; Immunology Unit, Hungary; email: ando@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Wang, WL AU - Liu, R AU - Zhang, T AU - Zhang, R AU - Song, X AU - Wang, LL AU - Song, LS TI - A novel phagocytic receptor (CgNimC) from Pacific oyster Crassostrea gigas with lipopolysaccharide and gram-negative bacteria binding activity JF - FISH AND SHELLFISH IMMUNOLOGY J2 - FISH SHELLFISH IMMUN VL - 43 PY - 2015 IS - 1 SP - 103 EP - 110 PG - 8 SN - 1050-4648 DO - 10.1016/j.fsi.2014.12.019 UR - https://m2.mtmt.hu/api/publication/24785129 ID - 24785129 N1 - Cited By :23 Export Date: 14 June 2022 CODEN: FSIME LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Varga, Gergely István AU - Honti, Viktor AU - Jankovics, Ferenc AU - Kurucz, Judit Éva AU - Andó, István TI - In Vivo Immunostaining of Hemocyte Compartments in Drosophila for Live Imaging JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 6 PG - 6 SN - 1932-6203 DO - 10.1371/journal.pone.0098191 UR - https://m2.mtmt.hu/api/publication/2708773 ID - 2708773 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Developmental Genetics Unit, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Cited By :5 Export Date: 31 January 2020 CODEN: POLNC Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Developmental Genetics Unit, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Cited By :8 Export Date: 20 April 2021 CODEN: POLNC AB - In recent years, Drosophila melanogaster has become an attractive model organism in which to study the structure and development of the cellular immune components. The emergence of immunological markers greatly accelerated the identification of the immune cells (hemocytes), while the creation of genetic reporter constructs allowed unique insight into the structural organization of hematopoietic tissues. However, investigation of the hemocyte compartments by the means of immunological markers requires dissection and fixation, which regularly disrupt the delicate structure and hamper the microanatomical characterization. Moreover, the investigation of transgenic reporters alone can be misleading as their expression often differs from the native expression pattern of their respective genes. We describe here a method that combines the reporter constructs and the immunological tools in live imaging, thereby allowing use of the array of available immunological markers while retaining the structural integrity of the hematopoietic compartments. The procedure allows the reversible immobilization of Drosophila larvae for high-resolution confocal imaging and the time-lapse video analysis of in vivo reporters. When combined with our antibody injection-based in situ immunostaining assay, the resulting double labeling of the hemocyte compartments can provide new information on the microanatomy and functional properties of the hematopoietic tissues in an intact state. Although this method was developed to study the immune system of Drosophila melanogaster, we anticipate that such a combination of genetic and immunological markers could become a versatile technique for in vivo studies in other biological systems too. LA - English DB - MTMT ER - TY - JOUR AU - Estevez-Lao, TY AU - Hillyer, JF TI - Involvement of the Anopheles gambiae Nimrod gene family in mosquito immune responses JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 44 PY - 2014 SP - 12 EP - 22 PG - 11 SN - 0965-1748 DO - 10.1016/j.ibmb.2013.10.008 UR - https://m2.mtmt.hu/api/publication/24097650 ID - 24097650 LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Csordás, Gábor AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Andó, István TI - The cell-mediated immunity of Drosophila melanogaster: Hemocyte lineages, immune compartments, microanatomy and regulation. JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 42 PY - 2014 IS - 1 SP - 47 EP - 56 PG - 10 SN - 0145-305X DO - 10.1016/j.dci.2013.06.005 UR - https://m2.mtmt.hu/api/publication/2372553 ID - 2372553 N1 - Cited By :124 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Andó, I.; Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary; email: ando.istvan@brc.mta.hu AB - In the animal kingdom, innate immunity is the first line of defense against invading pathogens. The dangers of microbial and parasitic attacks are countered by similar mechanisms, involving the prototypes of the cell-mediated immune responses, the phagocytosis and encapsulation. Work on Drosophila has played an important role in promoting an understanding of the basic mechanisms of phylogenetically conserved modules of innate immunity. The aim of this review is to survey the developments in the identification and functional definition of immune cell types and the immunological compartments of Drosophila melanogaster. We focus on the molecular and developmental aspects of the blood cell types and compartments, as well as the dynamics of blood cell development and the immune response. Further advances in the characterization of the innate immune mechanisms in Drosophila will provide basic clues to the understanding of the importance of the evolutionary conserved mechanisms of innate immune defenses in the animal kingdom. LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Kurucz, Judit Éva AU - Zsámboki, János AU - Evans, CJ AU - Banerjee, U AU - Andó, István TI - Variation of NimC1 expression in Drosophila stocks and transgenic strains. JF - FLY J2 - FLY VL - 7 PY - 2013 IS - 4 SP - 263 EP - 266 PG - 4 SN - 1933-6934 DO - 10.4161/fly.25654 UR - https://m2.mtmt.hu/api/publication/2372552 ID - 2372552 AB - The NimC1 molecule has been described as a phagocytosis receptor, and is being used as a marker for professional phagocytes, the plasmatocytes, in Drosophila melanogaster. In studies including tumor-biology, developmental biology, and cell mediated immunity, monoclonal antibodies (P1a and P1b) to the NimC1 antigen are used. As we observed that these antibodies did not react with plasmatocytes of several strains and genetic combinations, a molecular analysis was performed on the structure of the nimC1 gene. We found 2 deletions and an insertion within the nimC1 gene, which may result in the production of a truncated NimC1 protein. The NimC1 positivity was regained by recombining the mutation with a wild-type allele or by using nimC1 mutant lines under heterozygous conditions. By means of these procedures or gaining access to the recombined stock, NimC1 can be used as a marker for phagocytic cells in the majority of the possible genetic backgrounds. LA - English DB - MTMT ER - TY - JOUR AU - Kari, Beáta AU - Zsámboki, János AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Andó, István AU - Kurucz, Judit Éva TI - A novel method for the identification of factors involved in host-pathogen interactions in Drosophila melanogaster. JF - JOURNAL OF IMMUNOLOGICAL METHODS J2 - J IMMUNOL METHODS VL - 398-399 PY - 2013 SP - 76 EP - 82 PG - 7 SN - 0022-1759 DO - 10.1016/j.jim.2013.09.011 UR - https://m2.mtmt.hu/api/publication/2463198 ID - 2463198 N1 - WoS:hiba:000329011500009 2019-03-03 20:54 kötet nem egyezik AB - A new method was established, standardized and validated for screening factors involved in the response to septic injury in Drosophila melanogaster. The method, based on inducing lesion by removing the tarsal segments of the first pair of legs of Drosophila adults and exposing them to different bacteria, imitates injury that often occurs in the natural habitat. The method is easy to perform, highly reproducible and suitable for large-scale genetic screens with the aim of identifying factors involved in host-pathogen interactions. The technique was validated by using mutant variations of different components of the immune response, blood clotting as well as the involvement of a number of genes known to be instrumental in the humoral and cell-mediated immune responses of Drosophila was confirmed. Moreover, the combination of the present method with antibiotic treatment allows the screening of potential antimicrobial drugs in vivo. LA - English DB - MTMT ER -