@article{MTMT:32898786,
	title = {Sensing microbial infections in the Drosophila melanogaster genetic model organism},
	url = {https://m2.mtmt.hu/api/publication/32898786},
	author = {Liegeois, S. and Ferrandon, D.},
	doi = {10.1007/s00251-021-01239-0},
	journal-iso = {IMMUNOGENETICS},
	journal = {IMMUNOGENETICS},
	volume = {74},
	unique-id = {32898786},
	issn = {0093-7711},
	abstract = {Insects occupy a central position in the biosphere. They are able to resist infections even though they lack an adaptive immune system. Drosophila melanogaster has been used as a potent genetic model to understand innate immunity both in invertebrates and vertebrates. Its immune system includes both humoral and cellular arms. Here, we review how the distinct immune responses are triggered upon sensing infections, with an emphasis on the mechanisms that lead to systemic humoral immune responses. As in plants, the components of the cell wall of microorganisms are detected by dedicated receptors. There is also an induction of the systemic immune response upon sensing the proteolytic activities of microbial virulence factors. The antiviral response mostly relies on sensing double-stranded RNAs generated during the viral infection cycle. This event subsequently triggers either the viral short interfering RNA pathway or a cGAS-like/STING/NF-κB signaling pathway. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.},
	keywords = {Animals; metabolism; GENETICS; signal transduction; signal transduction; animal; innate immunity; innate immunity; Drosophila melanogaster; Drosophila melanogaster; Drosophila melanogaster; Models, Genetic; biological model; Immunity, Innate; NF-kappa B; immunoglobulin enhancer binding protein; Pattern recognition receptors; Effector-triggered immunity; Microbial infections; Pathogen-triggered immunity},
	year = {2022},
	eissn = {1432-1211},
	pages = {35-62}
}

@article{MTMT:32361576,
	title = {Haemocyte-mediated immunity in insects: Cells, processes and associated components in the fight against pathogens and parasites},
	url = {https://m2.mtmt.hu/api/publication/32361576},
	author = {Eleftherianos, Ioannis and Heryanto, Christa and Bassal, Taha and Zhang, Wei and Tettamanti, Gianluca and Mohamed, Amr},
	doi = {10.1111/imm.13390},
	journal-iso = {IMMUNOLOGY},
	journal = {IMMUNOLOGY},
	volume = {164},
	unique-id = {32361576},
	issn = {0019-2805},
	abstract = {The host defence of insects includes a combination of cellular and humoral responses. The cellular arm of the insect innate immune system includes mechanisms that are directly mediated by haemocytes (e.g., phagocytosis, nodulation and encapsulation). In addition, melanization accompanying coagulation, clot formation and wound healing, nodulation and encapsulation processes leads to the formation of cytotoxic redox-cycling melanin precursors and reactive oxygen and nitrogen species. However, demarcation between cellular and humoral immune reactions as two distinct categories is not straightforward. This is because many humoral factors affect haemocyte functions and haemocytes themselves are an important source of many humoral molecules. There is also a considerable overlap between cellular and humoral immune functions that span from recognition of foreign intruders to clot formation. Here, we review these immune reactions starting with the cellular mechanisms that limit haemolymph loss and participate in wound healing and clot formation and advancing to cellular functions that are critical in restricting pathogen movement and replication. This information is important because it highlights that insect cellular immunity is controlled by a multilayered system, different components of which are activated by different pathogens or during the different stages of the infection.},
	keywords = {haematopoiesis; haemocytes; prophenoloxidase; autophagy and apoptosis; cytotoxic intermediates; insect cellular immunity},
	year = {2021},
	eissn = {1365-2567},
	pages = {401-432},
	orcid-numbers = {Eleftherianos, Ioannis/0000-0002-4822-3110; Mohamed, Amr/0000-0003-2788-5534}
}

@article{MTMT:32585067,
	title = {Identification and the immunological role of two Nimrod family genes in the silkworm, Bombyx mori},
	url = {https://m2.mtmt.hu/api/publication/32585067},
	author = {Gul, Isma and Kausar, Saima and You, Qiuxiang and Sun, Wei and Li, Zekun and Abbas, Muhammad Nadeem and Cui, Hongjuan},
	doi = {10.1016/j.ijbiomac.2021.10.083},
	journal-iso = {INT J BIOL MACROMOL},
	journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
	volume = {193},
	unique-id = {32585067},
	issn = {0141-8130},
	year = {2021},
	eissn = {1879-0003},
	pages = {154-165}
}

@article{MTMT:32006366,
	title = {Dense time-course gene expression profiling of the Drosophila melanogaster innate immune response},
	url = {https://m2.mtmt.hu/api/publication/32006366},
	author = {Schlamp, F. and Delbare, S.Y.N. and Early, A.M. and Wells, M.T. and Basu, S. and Clark, A.G.},
	doi = {10.1186/s12864-021-07593-3},
	journal-iso = {BMC GENOMICS},
	journal = {BMC GENOMICS},
	volume = {22},
	unique-id = {32006366},
	issn = {1471-2164},
	abstract = {Background: Immune responses need to be initiated rapidly, and maintained as needed, to prevent establishment and growth of infections. At the same time, resources need to be balanced with other physiological processes. On the level of transcription, studies have shown that this balancing act is reflected in tight control of the initiation kinetics and shutdown dynamics of specific immune genes. Results: To investigate genome-wide expression dynamics and trade-offs after infection at a high temporal resolution, we performed an RNA-seq time course on D. melanogaster with 20 time points post Imd stimulation. A combination of methods, including spline fitting, cluster analysis, and Granger causality inference, allowed detailed dissection of expression profiles, lead-lag interactions, and functional annotation of genes through guilt-by-association. We identified Imd-responsive genes and co-expressed, less well characterized genes, with an immediate-early response and sustained up-regulation up to 5 days after stimulation. In contrast, stress response and Toll-responsive genes, among which were Bomanins, demonstrated early and transient responses. We further observed a strong trade-off with metabolic genes, which strikingly recovered to pre-infection levels before the immune response was fully resolved. Conclusions: This high-dimensional dataset enabled the comprehensive study of immune response dynamics through the parallel application of multiple temporal data analysis methods. The well annotated data set should also serve as a useful resource for further investigation of the D. melanogaster innate immune response, and for the development of methods for analysis of a post-stress transcriptional response time-series at whole-genome scale. © 2021, The Author(s).},
	keywords = {Drosophila melanogaster; immune response; Granger causality; Time course RNA-seq},
	year = {2021},
	eissn = {1471-2164}
}

@article{MTMT:30901164,
	title = {Phagocytosis in Drosophila: From molecules and cellular machinery to physiology},
	url = {https://m2.mtmt.hu/api/publication/30901164},
	author = {Melcarne, C. and Lemaitre, B. and Kurant, E.},
	doi = {10.1016/j.ibmb.2019.04.002},
	journal-iso = {INSECT BIOCHEM MOLEC},
	journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY},
	volume = {109},
	unique-id = {30901164},
	issn = {0965-1748},
	abstract = {Phagocytosis is an evolutionarily conserved mechanism that plays a key role in both host defence and tissue homeostasis in multicellular organisms. A range of surface receptors expressed on different cell types allow discriminating between self and non-self (or altered) material, thus enabling phagocytosis of pathogens and apoptotic cells. The phagocytosis process can be divided into four main steps: 1) binding of the phagocyte to the target particle, 2) particle internalization and phagosome formation, through remodelling of the plasma membrane, 3) phagosome maturation, and 4) particle destruction in the phagolysosome. In this review, we describe our present knowledge on phagocytosis in the fruit fly Drosophila melanogaster, assessing each of the key steps involved in engulfment of both apoptotic cells and bacteria. We also assess the physiological role of phagocytosis in host defence, development and tissue homeostasis.},
	year = {2019},
	eissn = {1879-0240},
	pages = {1-12}
}

@article{MTMT:30641961,
	title = {Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster},
	url = {https://m2.mtmt.hu/api/publication/30641961},
	author = {Melcarne, Claudia and Ramond, Elodie and Dudzic, Jan and Bretscher, Andrew and Kurucz, Judit Éva and Andó, István and Lemaitre, Bruno},
	doi = {10.1111/febs.14857},
	journal-iso = {FEBS J},
	journal = {FEBS JOURNAL},
	volume = {286},
	unique-id = {30641961},
	issn = {1742-464X},
	year = {2019},
	eissn = {1742-4658},
	pages = {2670-2691},
	orcid-numbers = {Andó, István/0000-0002-4648-9396}
}

@article{MTMT:27602651,
	title = {Eater and draper are involved in the periostial haemocyte immune response in the mosquito Anopheles gambiae},
	url = {https://m2.mtmt.hu/api/publication/27602651},
	author = {Sigle, L T and Hillyer, J F},
	doi = {10.1111/imb.12383},
	journal-iso = {INSECT MOL BIOL},
	journal = {INSECT MOLECULAR BIOLOGY},
	volume = {27},
	unique-id = {27602651},
	issn = {0962-1075},
	year = {2018},
	eissn = {1365-2583},
	pages = {429-438}
}

@article{MTMT:3249679,
	title = {Genes encoding cuticular proteins are components of the Nimrod gene cluster in Drosophila.},
	url = {https://m2.mtmt.hu/api/publication/3249679},
	author = {Cinege, Gyöngyi Ilona and Zsámboki, János and Vidal-Quadras, M and Uv, A and Csordás, Gábor and Honti, Viktor and Gábor, Erika and Hegedűs, Zoltán and Varga, Gergely István and Kovács, Attila Lajos and Juhász, Gábor and Williams, MJ and Andó, István and Kurucz, Judit Éva},
	doi = {10.1016/j.ibmb.2017.06.006},
	journal-iso = {INSECT BIOCHEM MOLEC},
	journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY},
	volume = {87},
	unique-id = {3249679},
	issn = {0965-1748},
	abstract = {The Nimrod gene cluster, located on the second chromosome of Drosophila melanogaster, is the largest synthenic unit of the Drosophila genome. Nimrod genes show blood cell specific expression and code for phagocytosis receptors that play a major role in fruit fly innate immune functions. We previously identified three homologous genes (vajk-1, vajk-2 and vajk-3) located within the Nimrod cluster, which are unrelated to the Nimrod genes, but are homologous to a fourth gene (vajk-4) located outside the cluster. Here we show that, unlike the Nimrod candidates, the Vajk proteins are expressed in cuticular structures of the late embryo and the late pupa, indicating that they contribute to cuticular barrier functions.},
	year = {2017},
	eissn = {1879-0240},
	pages = {45-54},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Varga, Gergely István/0000-0001-9073-5788; Juhász, Gábor/0000-0001-8548-8874; Andó, István/0000-0002-4648-9396}
}

@article{MTMT:25771630,
	title = {Insect immunology and hematopoiesis},
	url = {https://m2.mtmt.hu/api/publication/25771630},
	author = {Hillyer, Julian F},
	doi = {10.1016/j.dci.2015.12.006},
	journal-iso = {DEV COMP IMMUNOL},
	journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY},
	volume = {58},
	unique-id = {25771630},
	issn = {0145-305X},
	year = {2016},
	eissn = {1879-0089},
	pages = {102-118}
}

@article{MTMT:2993019,
	title = {Innate immunity},
	url = {https://m2.mtmt.hu/api/publication/2993019},
	author = {Honti, Viktor and Kurucz, Judit Éva and Cinege, Gyöngyi Ilona and Csordás, Gábor and Andó, István},
	journal-iso = {ACTA BIOL SZEGED},
	journal = {ACTA BIOLOGICA SZEGEDIENSIS},
	volume = {59},
	unique-id = {2993019},
	issn = {1588-385X},
	year = {2015},
	eissn = {1588-4082},
	pages = {1-15},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396}
}

@article{MTMT:24785129,
	title = {A novel phagocytic receptor (CgNimC) from Pacific oyster Crassostrea gigas with lipopolysaccharide and gram-negative bacteria binding activity},
	url = {https://m2.mtmt.hu/api/publication/24785129},
	author = {Wang, WL and Liu, R and Zhang, T and Zhang, R and Song, X and Wang, LL and Song, LS},
	doi = {10.1016/j.fsi.2014.12.019},
	journal-iso = {FISH SHELLFISH IMMUN},
	journal = {FISH AND SHELLFISH IMMUNOLOGY},
	volume = {43},
	unique-id = {24785129},
	issn = {1050-4648},
	year = {2015},
	eissn = {1095-9947},
	pages = {103-110}
}

@article{MTMT:2708773,
	title = {In Vivo Immunostaining of Hemocyte Compartments in Drosophila for Live Imaging},
	url = {https://m2.mtmt.hu/api/publication/2708773},
	author = {Csordás, Gábor and Varga, Gergely István and Honti, Viktor and Jankovics, Ferenc and Kurucz, Judit Éva and Andó, István},
	doi = {10.1371/journal.pone.0098191},
	journal-iso = {PLOS ONE},
	journal = {PLOS ONE},
	volume = {9},
	unique-id = {2708773},
	issn = {1932-6203},
	abstract = {In recent years, Drosophila melanogaster has become an attractive model organism in which to study the structure and development of the cellular immune components. The emergence of immunological markers greatly accelerated the identification of the immune cells (hemocytes), while the creation of genetic reporter constructs allowed unique insight into the structural organization of hematopoietic tissues. However, investigation of the hemocyte compartments by the means of immunological markers requires dissection and fixation, which regularly disrupt the delicate structure and hamper the microanatomical characterization. Moreover, the investigation of transgenic reporters alone can be misleading as their expression often differs from the native expression pattern of their respective genes. We describe here a method that combines the reporter constructs and the immunological tools in live imaging, thereby allowing use of the array of available immunological markers while retaining the structural integrity of the hematopoietic compartments. The procedure allows the reversible immobilization of Drosophila larvae for high-resolution confocal imaging and the time-lapse video analysis of in vivo reporters. When combined with our antibody injection-based in situ immunostaining assay, the resulting double labeling of the hemocyte compartments can provide new information on the microanatomy and functional properties of the hematopoietic tissues in an intact state. Although this method was developed to study the immune system of Drosophila melanogaster, we anticipate that such a combination of genetic and immunological markers could become a versatile technique for in vivo studies in other biological systems too.},
	year = {2014},
	eissn = {1932-6203},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Varga, Gergely István/0000-0001-9073-5788; Andó, István/0000-0002-4648-9396}
}

@article{MTMT:24097650,
	title = {Involvement of the Anopheles gambiae Nimrod gene family in mosquito immune responses},
	url = {https://m2.mtmt.hu/api/publication/24097650},
	author = {Estevez-Lao, TY and Hillyer, JF},
	doi = {10.1016/j.ibmb.2013.10.008},
	journal-iso = {INSECT BIOCHEM MOLEC},
	journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY},
	volume = {44},
	unique-id = {24097650},
	issn = {0965-1748},
	year = {2014},
	eissn = {1879-0240},
	pages = {12-22}
}

@article{MTMT:2372553,
	title = {The cell-mediated immunity of Drosophila melanogaster: Hemocyte lineages, immune compartments, microanatomy and regulation.},
	url = {https://m2.mtmt.hu/api/publication/2372553},
	author = {Honti, Viktor and Csordás, Gábor and Kurucz, Judit Éva and Márkus, Róbert and Andó, István},
	doi = {10.1016/j.dci.2013.06.005},
	journal-iso = {DEV COMP IMMUNOL},
	journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY},
	volume = {42},
	unique-id = {2372553},
	issn = {0145-305X},
	abstract = {In the animal kingdom, innate immunity is the first line of defense against invading pathogens. The dangers of microbial and parasitic attacks are countered by similar mechanisms, involving the prototypes of the cell-mediated immune responses, the phagocytosis and encapsulation. Work on Drosophila has played an important role in promoting an understanding of the basic mechanisms of phylogenetically conserved modules of innate immunity. The aim of this review is to survey the developments in the identification and functional definition of immune cell types and the immunological compartments of Drosophila melanogaster. We focus on the molecular and developmental aspects of the blood cell types and compartments, as well as the dynamics of blood cell development and the immune response. Further advances in the characterization of the innate immune mechanisms in Drosophila will provide basic clues to the understanding of the importance of the evolutionary conserved mechanisms of innate immune defenses in the animal kingdom.},
	year = {2014},
	eissn = {1879-0089},
	pages = {47-56},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396}
}

@article{MTMT:2372552,
	title = {Variation of NimC1 expression in Drosophila stocks and transgenic strains.},
	url = {https://m2.mtmt.hu/api/publication/2372552},
	author = {Honti, Viktor and Cinege, Gyöngyi Ilona and Csordás, Gábor and Kurucz, Judit Éva and Zsámboki, János and Evans, CJ and Banerjee, U and Andó, István},
	doi = {10.4161/fly.25654},
	journal-iso = {FLY},
	journal = {FLY},
	volume = {7},
	unique-id = {2372552},
	issn = {1933-6934},
	abstract = {The NimC1 molecule has been described as a phagocytosis receptor, and is being used as a marker for professional phagocytes, the plasmatocytes, in Drosophila melanogaster. In studies including tumor-biology, developmental biology, and cell mediated immunity, monoclonal antibodies (P1a and P1b) to the NimC1 antigen are used. As we observed that these antibodies did not react with plasmatocytes of several strains and genetic combinations, a molecular analysis was performed on the structure of the nimC1 gene. We found 2 deletions and an insertion within the nimC1 gene, which may result in the production of a truncated NimC1 protein. The NimC1 positivity was regained by recombining the mutation with a wild-type allele or by using nimC1 mutant lines under heterozygous conditions. By means of these procedures or gaining access to the recombined stock, NimC1 can be used as a marker for phagocytic cells in the majority of the possible genetic backgrounds.},
	year = {2013},
	eissn = {1933-6942},
	pages = {263-266},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396}
}

@article{MTMT:2463198,
	title = {A novel method for the identification of factors involved in host-pathogen interactions in Drosophila melanogaster.},
	url = {https://m2.mtmt.hu/api/publication/2463198},
	author = {Kari, Beáta and Zsámboki, János and Honti, Viktor and Csordás, Gábor and Márkus, Róbert and Andó, István and Kurucz, Judit Éva},
	doi = {10.1016/j.jim.2013.09.011},
	journal-iso = {J IMMUNOL METHODS},
	journal = {JOURNAL OF IMMUNOLOGICAL METHODS},
	volume = {398-399},
	unique-id = {2463198},
	issn = {0022-1759},
	abstract = {A new method was established, standardized and validated for screening factors involved in the response to septic injury in Drosophila melanogaster. The method, based on inducing lesion by removing the tarsal segments of the first pair of legs of Drosophila adults and exposing them to different bacteria, imitates injury that often occurs in the natural habitat. The method is easy to perform, highly reproducible and suitable for large-scale genetic screens with the aim of identifying factors involved in host-pathogen interactions. The technique was validated by using mutant variations of different components of the immune response, blood clotting as well as the involvement of a number of genes known to be instrumental in the humoral and cell-mediated immune responses of Drosophila was confirmed. Moreover, the combination of the present method with antibiotic treatment allows the screening of potential antimicrobial drugs in vivo.},
	year = {2013},
	eissn = {1872-7905},
	pages = {76-82},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396}
}