TY - JOUR AU - Bazzi, W. AU - Monticelli, S. AU - Delaporte, C. AU - Riet, C. AU - Giangrande, A. AU - Cattenoz, P.B. TI - Gcm counteracts Toll-induced inflammation and impacts hemocyte number through cholinergic signaling JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 SN - 1664-3224 DO - 10.3389/fimmu.2023.1293766 UR - https://m2.mtmt.hu/api/publication/34576476 ID - 34576476 N1 - Université de Strasbourg, IGBMC, UMR 7104- UMR-S 1258, Illkirch, France CNRS, UMR 7104, Illkirch, France Inserm, UMR-S 1258, Illkirch, France IGBMC, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France Export Date: 13 February 2024 Correspondence Address: Giangrande, A.; Université de Strasbourg, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Université de Strasbourg, France; email: cattenoz@igbmc.fr AB - Hemocytes, the myeloid-like immune cells of Drosophila, fulfill a variety of functions that are not completely understood, ranging from phagocytosis to transduction of inflammatory signals. We here show that downregulating the hemocyte-specific Glial cell deficient/Glial cell missing (Glide/Gcm) transcription factor enhances the inflammatory response to the constitutive activation of the Toll pathway. This correlates with lower levels of glutathione S-transferase, suggesting an implication of Glide/Gcm in reactive oxygen species (ROS) signaling and calling for a widespread anti-inflammatory potential of Glide/Gcm. In addition, our data reveal the expression of acetylcholine receptors in hemocytes and that Toll activation affects their expressions, disclosing a novel aspect of the inflammatory response mediated by neurotransmitters. Finally, we provide evidence for acetylcholine receptor nicotinic acetylcholine receptor alpha 6 (nAchRalpha6) regulating hemocyte proliferation in a cell autonomous fashion and for non-cell autonomous cholinergic signaling regulating the number of hemocytes. Altogether, this study provides new insights on the molecular pathways involved in the inflammatory response. Copyright © 2023 Bazzi, Monticelli, Delaporte, Riet, Giangrande and Cattenoz. LA - English DB - MTMT ER - TY - JOUR AU - Johannessen, J.A. AU - Formica, M. AU - Haukeland, A.L.C. AU - Bråthen, N.R. AU - Al, Outa A. AU - Aarsund, M. AU - Therrien, M. AU - Enserink, J.M. AU - Knævelsrud, H. TI - The human leukemic oncogene MLL-AF4 promotes hyperplastic growth of hematopoietic tissues in Drosophila larvae JF - ISCIENCE J2 - ISCIENCE VL - 26 PY - 2023 IS - 10 SN - 2589-0042 DO - 10.1016/j.isci.2023.107726 UR - https://m2.mtmt.hu/api/publication/34576395 ID - 34576395 N1 - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway Department of Molecular Medicine, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway Institute for Research in Immunology and Cancer, Laboratory of Intracellular Signaling, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Montréal, QC H3C 3J7, Canada Département de pathologie et de biologie cellulaire, Université de Montréal, Montréal, QC H3C 3J7, Canada Section for Biochemistry and Molecular Biology, The Department of Biosciences, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway Export Date: 13 February 2024 Correspondence Address: Knævelsrud, H.; Department of Molecular Cell Biology, Norway; email: helene.knavelsrud@medisin.uio.no AB - MLL-rearranged (MLL-r) leukemias are among the leukemic subtypes with poorest survival, and treatment options have barely improved over the last decades. Despite increasing molecular understanding of the mechanisms behind these hematopoietic malignancies, this knowledge has had poor translation into the clinic. Here, we report a Drosophila melanogaster model system to explore the pathways affected in MLL-r leukemia. We show that expression of the human leukemic oncogene MLL-AF4 in the Drosophila hematopoietic system resulted in increased levels of circulating hemocytes and an enlargement of the larval hematopoietic organ, the lymph gland. Strikingly, depletion of Drosophila orthologs of known interactors of MLL-AF4, such as DOT1L, rescued the leukemic phenotype. In agreement, treatment with small-molecule inhibitors of DOT1L also prevented the MLL-AF4-induced leukemia-like phenotype. Taken together, this model provides an in vivo system to unravel the genetic interactors involved in leukemogenesis and offers a system for improved biological understanding of MLL-r leukemia. © 2023 The Author(s) LA - English DB - MTMT ER - TY - JOUR AU - Kúthy-Sutus, Enikő AU - Kharrat, Bayan AU - Gábor, Erika AU - Csordás, Gábor AU - Sinka, Rita AU - Honti, Viktor TI - A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 1 PG - 15 SN - 2073-4409 DO - 10.3390/cells12010024 UR - https://m2.mtmt.hu/api/publication/33555087 ID - 33555087 N1 - Export Date: 24 January 2023 AB - The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo. LA - English DB - MTMT ER - TY - JOUR AU - Bakopoulos, D. AU - Whisstock, J.C. AU - Warr, C.G. AU - Johnson, T.K. TI - Macrophage self-renewal is regulated by transient expression of PDGF- and VEGF-related factor 2 JF - FEBS JOURNAL J2 - FEBS J VL - 289 PY - 2022 IS - 13 SP - 3735 EP - 3751 PG - 17 SN - 1742-464X DO - 10.1111/febs.16364 UR - https://m2.mtmt.hu/api/publication/32876827 ID - 32876827 N1 - Export Date: 14 June 2022 CODEN: FJEOA AB - Macrophages are an ancient blood cell lineage critical for homeostasis and defence against pathogens. Although their numbers were long thought to be sustained solely by haematopoietic organs, it has recently become clear that their proliferation, or self-renewal, also plays a major role. In the Drosophila larva, macrophages undergo a phase of rapid self-renewal, making this an attractive model for elucidating the signals and regulatory mechanisms involved. However, a central self-renewal pathway has not been identified in this system. Here, we show that the PDGF- and VEGF-receptor related (Pvr) pathway fulfils this role. Our data show that two of the three known Pvr ligands, PDGF- and VEGF-related factor 2 (Pvf2) and Pvf3, are major determinants of overall macrophage numbers, yet they each act in a temporally independent manner and via distinct mechanisms. While Pvf3 is needed prior to the self-renewal period, we find that Pvf2 is critical specifically for expanding the larval macrophage population. We further show that Pvf2 is a potent macrophage mitogen that is kept at limiting quantities by its transient expression in a remarkably small number of blood cells. Together, these data support a novel mechanism for the regulation of macrophage self-renewal rates by the dynamic transcriptional control of Pvf2. Given the strong parallels that exist between Drosophila and vertebrate macrophage systems, it is likely that a similar self-renewal control mechanism is at play across animal phyla. © 2022 Federation of European Biochemical Societies LA - English DB - MTMT ER - TY - JOUR AU - Feng, M. AU - Swevers, L. AU - Sun, J. TI - Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 13 PY - 2022 SN - 1664-3224 DO - 10.3389/fimmu.2022.852702 UR - https://m2.mtmt.hu/api/publication/32876826 ID - 32876826 N1 - Export Date: 14 June 2022 AB - Within the hemolymph, insect hemocytes constitute a heterogeneous population of macrophage-like cells that play important roles in innate immunity, homeostasis and development. Classification of hemocytes in different subtypes by size, morphology and biochemical or immunological markers has been difficult and only in Drosophila extensive genetic analysis allowed the construction of a coherent picture of hemocyte differentiation from pro-hemocytes to granulocytes, crystal cells and plasmatocytes. However, the advent of high-throughput single cell technologies, such as single cell RNA sequencing (scRNA-seq), is bound to have a high impact on the study of hemocytes subtypes and their phenotypes in other insects for which a sophisticated genetic toolbox is not available. Instead of averaging gene expression across all cells as occurs in bulk-RNA-seq, scRNA-seq allows high-throughput and specific visualization of the differentiation status of individual cells. With scRNA-seq, interesting cell types can be identified in heterogeneous populations and direct analysis of rare cell types is possible. Next to its ability to profile the transcriptomes of individual cells in tissue samples, scRNA-seq can be used to propose marker genes that are characteristic of different hemocyte subtypes and predict their functions. In this perspective, the identities of the different marker genes that were identified by scRNA-seq analysis to define 13 distinct cell clusters of hemocytes in larvae of the silkworm, Bombyx mori, are discussed in detail. The analysis confirms the broad division of hemocytes in granulocytes, plasmatocytes, oenocytoids and perhaps spherulocytes but also reveals considerable complexity at the molecular level and highly specialized functions. In addition, predicted hemocyte marker genes in Bombyx generally show only limited convergence with the genes that are considered characteristic for hemocyte subtypes in Drosophila. Copyright © 2022 Feng, Swevers and Sun. LA - English DB - MTMT ER - TY - JOUR AU - Hultmark, Dan AU - Andó, István TI - Hematopoietic plasticity mapped in Drosophila and other insects JF - ELIFE J2 - ELIFE VL - 11 PY - 2022 PG - 36 SN - 2050-084X DO - 10.7554/eLife.78906 UR - https://m2.mtmt.hu/api/publication/33039275 ID - 33039275 N1 - Funding Agency and Grant Number: Vetenskapsradet [2018-05114]; Hungarian Science Foundation [K135877] Funding text: Vetenskapsradet 2018-05114 Dan HultmarkHungarian Science Foundation K135877 Istvan AndoThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. LA - English DB - MTMT ER - TY - JOUR AU - Kharrat, Bayan AU - Csordás, Gábor AU - Honti, Viktor TI - Peeling Back the Layers of Lymph Gland Structure and Regulation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 14 PG - 16 SN - 1661-6596 DO - 10.3390/ijms23147767 UR - https://m2.mtmt.hu/api/publication/33050458 ID - 33050458 N1 - Cited By :2 Export Date: 24 January 2023 AB - During the past 60 years, the fruit fly, Drosophila melanogaster, has proven to be an excellent model to study the regulation of hematopoiesis. This is not only due to the evolutionarily conserved signalling pathways and transcription factors contributing to blood cell fate, but also to convergent evolution that led to functional similarities in distinct species. An example of convergence is the compartmentalization of blood cells, which ensures the quiescence of hematopoietic stem cells and allows for the rapid reaction of the immune system upon challenges. The lymph gland, a widely studied hematopoietic organ of the Drosophila larva, represents a microenvironment with similar features and functions to classical hematopoietic stem cell niches of vertebrates. Lymph gland studies were effectively supported by the unparalleled toolkit developed in Drosophila, which enabled the high-resolution investigation of the cellular composition and regulatory interaction networks of the lymph gland. In this review, we summarize how our understanding of lymph gland structure and hematopoietic cell-to-cell communication evolved during the past decades and compare their analogous features to those of the vertebrate hematopoietic stem cell niche. LA - English DB - MTMT ER - TY - JOUR AU - Balog, József Ágoston AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Kari, Beáta AU - Puskás, László AU - Andó, István AU - Szebeni, Gábor TI - Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry JF - GENOMICS PROTEOMICS & BIOINFORMATICS J2 - GENOM PROTEOM BIOINF VL - 19 PY - 2021 IS - 2 SP - 243 EP - 252 PG - 10 SN - 1672-0229 DO - 10.1016/j.gpb.2020.06.022 UR - https://m2.mtmt.hu/api/publication/31940120 ID - 31940120 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030, GINOP-2.3.2-15-2016-00035, NKFI NN118207, NKFI K120142, NKFI 120140, OTKA K-131484]; New National Excellence Program of the Ministry for Innovation and Technology, Hungary [UNKP-19-4-SZTE-36]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [BO/00139/17/8] Funding text: This work was supported by the grants from the National Research, Development and Innovation Office, Hungary (Grant Nos. GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030 to LGP, GINOP-2.3.2-15-2016-00035 to E ' K, NKFI NN118207 and NKFI K120142 to IA, NKFI 120140 to E ' K, and OTKA K-131484 to VH). Ga ' bor J. Szebeni was supported by the New National Excellence Program of the Ministry for Innovation and Technology, Hungary (Grant No. UNKP-19-4-SZTE-36) and by the Ja ' nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (Grant No. BO/00139/17/8). We are grateful to Mrs. Olga Kovalcsik for the technical help. LA - English DB - MTMT ER - TY - JOUR AU - Boulet, M. AU - Renaud, Y. AU - Lapraz, F. AU - Benmimoun, B. AU - Vandel, L. AU - Waltzer, L. TI - Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 9 PY - 2021 SN - 2296-634X DO - 10.3389/fcell.2021.739357 UR - https://m2.mtmt.hu/api/publication/32601830 ID - 32601830 N1 - Université Clermont Auvergne, Centre National de la Recherche Scientifique, Institut National de la Sante et de la Recherche Medicale, Institut Génétique Reproduction et Développement, Clermont-Ferrand, France Centre de Biologie du Développement, Centre de Biologie Intégrative, Université de Toulouse, Centre National de la Recherche Scientifique, Université Paul Sabatier, Toulouse, France Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut National de la Sante et de la Recherche Medicale, Institut de Biologie Valrose, Nice, France Institut Pasteur, Centre National de la Recherche Scientifique, UMR 3738, Paris, France Export Date: 19 January 2022 Correspondence Address: Waltzer, L.; Université Clermont Auvergne, France; email: lucas.waltzer@uca.fr AB - While many studies have described Drosophila embryonic and larval blood cells, the hematopoietic system of the imago remains poorly characterized and conflicting data have been published concerning adult hematopoiesis. Using a combination of blood cell markers, we show that the adult hematopoietic system is essentially composed of a few distinct mature blood cell types. In addition, our transcriptomics results indicate that adult and larval blood cells have both common and specific features and it appears that adult hemocytes reactivate many genes expressed in embryonic blood cells. Interestingly, we identify a small set of blood cells that does not express differentiation markers but rather maintains the expression of the progenitor marker domeMeso. Yet, we show that these cells are derived from the posterior signaling center, a specialized population of cells present in the larval lymph gland, rather than from larval blood cell progenitors, and that their maintenance depends on the EBF transcription factor Collier. Furthermore, while these cells are normally quiescent, we find that some of them can differentiate and proliferate in response to bacterial infection. In sum, our results indicate that adult flies harbor a small population of specialized cells with limited hematopoietic potential and further support the idea that no substantial hematopoiesis takes place during adulthood. © Copyright © 2021 Boulet, Renaud, Lapraz, Benmimoun, Vandel and Waltzer. LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Gábor, Erika AU - Honti, Viktor TI - There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 469 PY - 2021 SP - 135 EP - 143 PG - 9 SN - 0012-1606 DO - 10.1016/j.ydbio.2020.10.006 UR - https://m2.mtmt.hu/api/publication/31743832 ID - 31743832 N1 - Cited By :5 Export Date: 14 June 2022 CODEN: DEBIA LA - English DB - MTMT ER - TY - JOUR AU - Eleftherianos, Ioannis AU - Heryanto, Christa AU - Bassal, Taha AU - Zhang, Wei AU - Tettamanti, Gianluca AU - Mohamed, Amr TI - Haemocyte-mediated immunity in insects: Cells, processes and associated components in the fight against pathogens and parasites JF - IMMUNOLOGY J2 - IMMUNOLOGY VL - 164 PY - 2021 IS - 3 SP - 401 EP - 432 PG - 32 SN - 0019-2805 DO - 10.1111/imm.13390 UR - https://m2.mtmt.hu/api/publication/32361576 ID - 32361576 N1 - Infection and Innate Immunity Laboratory, Department of Biological Sciences, Institute for Biomedical Sciences, The George Washington University, Washington, DC, United States Department of Entomology, Faculty of Science, Cairo University, Giza, Egypt State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Napoli Federico II, Napoli, Italy Cited By :4 Export Date: 19 January 2022 CODEN: IMMUA Correspondence Address: Eleftherianos, I.; Infection and Innate Immunity Laboratory, United States; email: ioannise@gwu.edu Correspondence Address: Mohamed, A.; Department of Entomology, Egypt; email: mamr@sci.cu.edu.eg AB - The host defence of insects includes a combination of cellular and humoral responses. The cellular arm of the insect innate immune system includes mechanisms that are directly mediated by haemocytes (e.g., phagocytosis, nodulation and encapsulation). In addition, melanization accompanying coagulation, clot formation and wound healing, nodulation and encapsulation processes leads to the formation of cytotoxic redox-cycling melanin precursors and reactive oxygen and nitrogen species. However, demarcation between cellular and humoral immune reactions as two distinct categories is not straightforward. This is because many humoral factors affect haemocyte functions and haemocytes themselves are an important source of many humoral molecules. There is also a considerable overlap between cellular and humoral immune functions that span from recognition of foreign intruders to clot formation. Here, we review these immune reactions starting with the cellular mechanisms that limit haemolymph loss and participate in wound healing and clot formation and advancing to cellular functions that are critical in restricting pathogen movement and replication. This information is important because it highlights that insect cellular immunity is controlled by a multilayered system, different components of which are activated by different pathogens or during the different stages of the infection. LA - English DB - MTMT ER - TY - JOUR AU - Frankenreiter, L. AU - Gahr, B.M. AU - Schmid, H. AU - Zimmermann, M. AU - Deichsel, S. AU - Hoffmeister, P. AU - Turkiewicz, A. AU - Borggrefe, T. AU - Oswald, F. AU - Nagel, A.C. TI - Phospho-Site Mutations in Transcription Factor Suppressor of Hairless Impact Notch Signaling Activity During Hematopoiesis in Drosophila JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 9 PY - 2021 SN - 2296-634X DO - 10.3389/fcell.2021.658820 UR - https://m2.mtmt.hu/api/publication/32006305 ID - 32006305 N1 - Department of General Genetics (190g), Institute of Biology (190), University of Hohenheim, Stuttgart, Germany Department of Internal Medicine 1, Center for Internal Medicine, University Medical Center Ulm, Ulm, Germany Institute of Biochemistry, Justus-Liebig University of Giessen, Giessen, Germany Export Date: 10 May 2021 Correspondence Address: Nagel, A.C.; Department of General Genetics (190g), Germany; email: anja.nagel@uni-hohenheim.de AB - The highly conserved Notch signaling pathway controls a multitude of developmental processes including hematopoiesis. Here, we provide evidence for a novel mechanism of tissue-specific Notch regulation involving phosphorylation of CSL transcription factors within the DNA-binding domain. Earlier we found that a phospho-mimetic mutation of the Drosophila CSL ortholog Suppressor of Hairless [Su(H)] at Ser269 impedes DNA-binding. By genome-engineering, we now introduced phospho-specific Su(H) mutants at the endogenous Su(H) locus, encoding either a phospho-deficient [Su(H)S269A] or a phospho-mimetic [Su(H)S269D] isoform. Su(H)S269D mutants were defective of Notch activity in all analyzed tissues, consistent with impaired DNA-binding. In contrast, the phospho-deficient Su(H)S269A mutant did not generally augment Notch activity, but rather specifically in several aspects of blood cell development. Unexpectedly, this process was independent of the corepressor Hairless acting otherwise as a general Notch antagonist in Drosophila. This finding is in agreement with a novel mode of Notch regulation by posttranslational modification of Su(H) in the context of hematopoiesis. Importantly, our studies of the mammalian CSL ortholog (RBPJ/CBF1) emphasize a potential conservation of this regulatory mechanism: phospho-mimetic RBPJS221D was dysfunctional in both the fly as well as two human cell culture models, whereas phospho-deficient RBPJS221A rather gained activity during fly hematopoiesis. Thus, dynamic phosphorylation of CSL-proteins within the DNA-binding domain provides a novel means to fine-tune Notch signal transduction in a context-dependent manner. © Copyright © 2021 Frankenreiter, Gahr, Schmid, Zimmermann, Deichsel, Hoffmeister, Turkiewicz, Borggrefe, Oswald and Nagel. LA - English DB - MTMT ER - TY - JOUR AU - Morin-Poulard, Ismael AU - Tian, Yushun AU - Vanzo, Nathalie AU - Crozatier, Michele TI - Drosophila as a Model to Study Cellular Communication Between the Hematopoietic Niche and Blood Progenitors Under Homeostatic Conditions and in Response to an Immune Stress JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 PG - 11 SN - 1664-3224 DO - 10.3389/fimmu.2021.719349 UR - https://m2.mtmt.hu/api/publication/32361805 ID - 32361805 N1 - Export Date: 19 January 2022 Correspondence Address: Crozatier, M.; MCD, France AB - In adult mammals, blood cells are formed from hematopoietic stem progenitor cells, which are controlled by a complex cellular microenvironment called "niche". Drosophila melanogaster is a powerful model organism to decipher the mechanisms controlling hematopoiesis, due both to its limited number of blood cell lineages and to the conservation of genes and signaling pathways throughout bilaterian evolution. Insect blood cells or hemocytes are similar to the mammalian myeloid lineage that ensures innate immunity functions. Like in vertebrates, two waves of hematopoiesis occur in Drosophila. The first wave takes place during embryogenesis. The second wave occurs at larval stages, where two distinct hematopoietic sites are identified: subcuticular hematopoietic pockets and a specialized hematopoietic organ called the lymph gland. In both sites, hematopoiesis is regulated by distinct niches. In hematopoietic pockets, sensory neurons of the peripheral nervous system provide a microenvironment that promotes embryonic hemocyte expansion and differentiation. In the lymph gland blood cells are produced from hematopoietic progenitors. A small cluster of cells called Posterior Signaling Centre (PSC) and the vascular system, along which the lymph gland develops, act collectively as a niche, under homeostatic conditions, to control the balance between maintenance and differentiation of lymph gland progenitors. In response to an immune stress such as wasp parasitism, lymph gland hematopoiesis is drastically modified and shifts towards emergency hematopoiesis, leading to increased progenitor proliferation and their differentiation into lamellocyte, a specific blood cell type which will neutralize the parasite. The PSC is essential to control this emergency response. In this review, we summarize Drosophila cellular and molecular mechanisms involved in the communication between the niche and hematopoietic progenitors, both under homeostatic and stress conditions. Finally, we discuss similarities between mechanisms by which niches regulate hematopoietic stem/progenitor cells in Drosophila and mammals. LA - English DB - MTMT ER - TY - JOUR AU - Rodrigues, D. AU - VijayRaghavan, K. AU - Waltzer, L. AU - Inamdar, M.S. TI - Intact in situ Preparation of the Drosophila melanogaster Lymph Gland for a Comprehensive Analysis of Larval Hematopoiesis JF - BIO-PROTOCOL J2 - BIO-PROTOCOL VL - 11 PY - 2021 IS - 21 SN - 2331-8325 DO - 10.21769/BioProtoc.4204 UR - https://m2.mtmt.hu/api/publication/32601829 ID - 32601829 N1 - Export Date: 19 January 2022 Correspondence Address: Inamdar, M.S.; Jawaharlal Nehru Centre for Advanced Scientific ResearchIndia; email: inamdar@jncasr.ac.in AB - [Abstract] Blood cells have a limited lifespan and are replenished by a small number of hematopoietic stem and progenitor cells (HSPCs). Adult vertebrate hematopoiesis occurs in the bone marrow, liver, and spleen, rendering a comprehensive analysis of the entire HSPC pool nearly impossible. The Drosophila blood system is well studied and has developmental, molecular, and functional parallels with that of vertebrates. Unlike vertebrates, post-embryonic hematopoiesis in Drosophila is essentially restricted to the larval lymph gland (LG), a multi-lobed organ that flanks the dorsal vessel. Because the anterior-most or primary lobes of the LG are easy to dissect out, their cellular and molecular characteristics have been studied in considerable detail. The 2-3 pairs of posterior lobes are more delicate and fragile and have largely been ignored. However, posterior lobes harbor a significant blood progenitor pool, and several hematopoietic mutants show differences in phenotype between the anterior and posterior lobes. Hence, a comprehensive analysis of the LG is important for a thorough understanding of Drosophila hematopoiesis. Most studies focus on isolating the primary lobes by methods that generally dislodge and damage other lobes. To obtain preparations of the whole LG, including intact posterior lobes, here we provide a detailed protocol for larval fillet dissection. This allows accessing and analyzing complete LG lobes, along with dorsal vessel and pericardial cells. We demonstrate that tissue architecture and integrity is maintained and provide methods for quantitative analysis. This protocol can be used to quickly and effectively isolate complete LGs from first instar larval to pupal stages and can be implemented with ease. © 2021 Bio-protocol LLC. All Rights Reserved. LA - English DB - MTMT ER - TY - JOUR AU - Szkalisity, Ábel AU - Piccinini, Filippo AU - Beleon, Attila AU - Balassa, Tamás AU - Varga, Gergely István AU - Migh, Ede AU - Molnár, Csaba AU - Paavolainen, Lassi AU - Timonen, Sanna AU - Banerjee, Indranil AU - Ikonen, Elina AU - Yamauchi, Yohei AU - Andó, István AU - Peltonen, Jaakko AU - Pietiäinen, Vilja AU - Honti, Viktor AU - Horváth, Péter TI - Regression plane concept for analysing continuous cellular processes with machine learning JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 12 PY - 2021 IS - 1 PG - 9 SN - 2041-1723 DO - 10.1038/s41467-021-22866-x UR - https://m2.mtmt.hu/api/publication/32023128 ID - 32023128 N1 - Cited By :2 Export Date: 14 June 2022 LA - English DB - MTMT ER - TY - JOUR AU - Trainor, J.E. AU - Pooja, K.R. AU - Mortimer, N.T. TI - Immune cell production is targeted by parasitoid wasp virulence in a drosophila–parasitoid wasp interaction JF - PATHOGENS J2 - PATHOGENS VL - 10 PY - 2021 IS - 1 SP - 1 EP - 16 PG - 16 SN - 2076-0817 DO - 10.3390/pathogens10010049 UR - https://m2.mtmt.hu/api/publication/31868552 ID - 31868552 N1 - Export Date: 12 February 2021 Correspondence Address: Mortimer, N.T.; School of Biological Sciences, United States; email: ntmorti@ilstu.edu Export Date: 14 February 2021 Correspondence Address: Mortimer, N.T.; School of Biological Sciences, United States; email: ntmorti@ilstu.edu AB - The interactions between Drosophila melanogaster and the parasitoid wasps that infect Drosophila species provide an important model for understanding host–parasite relationships. Following parasitoid infection, D. melanogaster larvae mount a response in which immune cells (hemocytes) form a capsule around the wasp egg, which then melanizes, leading to death of the parasitoid. Previous studies have found that host hemocyte load; the number of hemocytes available for the encapsulation response; and the production of lamellocytes, an infection induced hemocyte type, are major determinants of host resistance. Parasitoids have evolved various virulence mechanisms to overcome the immune response of the D. melanogaster host, including both active immune suppression by venom proteins and passive immune evasive mechanisms. We identified a previously undescribed parasitoid species, Asobara sp. AsDen, which utilizes an active virulence mechanism to infect D. melanogaster hosts. Asobara sp. AsDen infection inhibits host hemocyte expression of msn, a member of the JNK signaling pathway, which plays a role in lamellocyte production. Asobara sp. AsDen infection restricts the production of lamellocytes as assayed by hemocyte cell morphology and altered msn expression. Our findings suggest that Asobara sp. AsDen infection alters host signaling to suppress immunity. © 2021 by the authors. Li-censee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Wan, Bin AU - Belghazi, Maya AU - Lemauf, Severine AU - Poirie, Marylene AU - Gatti, Jean-Luc TI - Proteomics of purified lamellocytes from Drosophila melanogaster HopTum-l identifies new membrane proteins and networks involved in their functions JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 134 PY - 2021 PG - 21 SN - 0965-1748 DO - 10.1016/j.ibmb.2021.103584 UR - https://m2.mtmt.hu/api/publication/32358968 ID - 32358968 N1 - Université Côte d'Azur, INRAE, CNRS, Institute Sophia-Agrobiotech, Sophia Antipolis, France Institute of NeuroPhysiopathology (INP), UMR7051, CNRS, Aix-Marseille Université, Marseille, 13015, France Cited By :1 Export Date: 19 January 2022 CODEN: IBMBE Correspondence Address: Gatti, J.-L.; Sophia Agrobiotech Institute (ISA), Sophia Antipolis, France; email: jean-luc.gatti@inrae.fr AB - In healthy Drosophila melanogaster larvae, plasmatocytes and crystal cells account for 95% and 5% of the hemocytes, respectively. A third type of hemocytes, lamellocytes, are rare, but their number increases after oviposition by parasitoid wasps. The lamellocytes form successive layers around the parasitoid egg, leading to its encapsulation and melanization, and finally the death of this intruder. However, the total number of lamellocytes per larva remains quite low even after parasitoid infestation, making direct biochemical studies difficult. Here, we used the HopTum-l mutant strain that constitutively produces large numbers of lamellocytes to set up a purification method and analyzed their major proteins by 2D gel electrophoresis and their plasma membrane surface proteins by 1D SDS-PAGE after affinity purification. Mass spectrometry identified 430 proteins from 2D spots and 344 affinity-purified proteins from 1D bands, for a total of 639 unique proteins. Known lamellocyte markers such as PPO3 and the myospheroid integrin were among the components identified with specific chaperone proteins. Affinity purification detected other integrins, as well as a wide range of integrin-associated proteins involved in the formation and function of cell-cell junctions. Overall, the newly identified proteins indicate that these cells are highly adapted to the encapsulation process (recognition, motility, adhesion, signaling), but may also have several other physiological functions (such as secretion and internalization of vesicles) under different signaling pathways. These results provide the basis for further in vivo and in vitro studies of lamellocytes, including the development of new markers to identify coexisting populations and their respective origins and functions in Drosophila immunity. LA - English DB - MTMT ER - TY - JOUR AU - Yang, L. AU - Qiu, L.-M. AU - Fang, Q. AU - Stanley, D.W. AU - Ye, G.-Y. TI - Cellular and humoral immune interactions between Drosophila and its parasitoids JF - INSECT SCIENCE J2 - INSECT SCI VL - 28 PY - 2021 IS - 5 SP - 1208 EP - 1227 PG - 20 SN - 1672-9609 DO - 10.1111/1744-7917.12863 UR - https://m2.mtmt.hu/api/publication/31596046 ID - 31596046 N1 - State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China USDA Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, MO, United States Cited By :28 Export Date: 15 February 2024 Correspondence Address: Ye, G.-Y.; State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, China; email: chu@zju.edu.cn AB - The immune interactions occurring between parasitoids and their host insects, especially in Drosophila–wasp models, have long been the research focus of insect immunology and parasitology. Parasitoid infestation in Drosophila is counteracted by its multiple natural immune defense systems, which include cellular and humoral immunity. Occurring in the hemocoel, cellular immune responses involve the proliferation, differentiation, migration and spreading of host hemocytes and parasitoid encapsulation by them. Contrastingly, humoral immune responses rely more heavily on melanization and on the Toll, Imd and Jak/Stat immune pathways associated with antimicrobial peptides along with stress factors. On the wasps’ side, successful development is achieved by introducing various virulence factors to counteract immune responses of Drosophila. Some or all of these factors manipulate the host's immunity for successful parasitism. Here we review current knowledge of the cellular and humoral immune interactions between Drosophila and its parasitoids, focusing on the defense mechanisms used by Drosophila and the strategies evolved by parasitic wasps to outwit it. © 2020 Institute of Zoology, Chinese Academy of Sciences LA - English DB - MTMT ER - TY - JOUR AU - Bozler, Julianna AU - Kacsoh, Balint Z. AU - Bosco, Giovanni TI - Maternal Priming of Offspring Immune System in Drosophila JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 10 PY - 2020 IS - 1 SP - 165 EP - 175 PG - 11 SN - 2160-1836 DO - 10.1534/g3.119.400852 UR - https://m2.mtmt.hu/api/publication/31468836 ID - 31468836 N1 - Cited By :7 Export Date: 19 January 2022 Correspondence Address: Bozler, J.; Dartmouth Medical School, Vail 609, 74 College St, United States; email: Lita.Kacsoh@gmail.com AB - Immune priming occurs when a past infection experience leads to a more effective immune response upon a secondary exposure to the infection or pathogen. In some instances, parents are able to transmit immune priming to their offspring, creating a subsequent generation with a superior immune capability, through processes that are not yet fully understood. Using a parasitoid wasp, which infects larval stages of Drosophila melanogaster, we describe an example of an intergenerational inheritance of immune priming. This phenomenon is anticipatory in nature and does not rely on parental infection, but rather, when adult fruit flies are cohabitated with a parasitic wasp, they produce offspring that are more capable of mounting a successful immune response against a parasitic macro-infection. This increase in offspring survival correlates with a more rapid induction of lamellocytes, a specialized immune cell. RNA-sequencing of the female germline identifies several differentially expressed genes following wasp exposure, including the peptiodoglycan recognition protein-LB (PGRP-LB). We find that genetic manipulation of maternal PGRP-LB identifies this gene as a key element in this intergenerational phenotype. LA - English DB - MTMT ER - TY - JOUR AU - Cattenoz, Pierre B. AU - Sakr, Rosy AU - Pavlidaki, Alexia AU - Delaporte, Claude AU - Riba, Andrea AU - Molina, Nacho AU - Hariharan, Nivedita AU - Mukherjee, Tina AU - Giangrande, Angela TI - Temporal specificity and heterogeneity ofDrosophilaimmune cells JF - EMBO JOURNAL J2 - EMBO J VL - 39 PY - 2020 IS - 12 PG - 25 SN - 0261-4189 DO - 10.15252/embj.2020104486 UR - https://m2.mtmt.hu/api/publication/31460653 ID - 31460653 N1 - Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France Centre National de la Recherche Scientifique, UMR7104, Illkirch, France Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France Université de Strasbourg, Illkirch, France Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, India The University of Trans-disciplinary Health Sciences and Technology, Bangalore, India Cited By :31 Export Date: 19 January 2022 CODEN: EMJOD Correspondence Address: Cattenoz, P.B.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Centre National de la Recherche Scientifique, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Centre National de la Recherche Scientifique, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Institut National de la Santé et de la Recherche Médicale, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut National de la Santé et de la Recherche Médicale, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Université de StrasbourgFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Université de StrasbourgFrance; email: angela@igbmc.fr AB - Immune cells provide defense against non-self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity ofDrosophilaimmune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single-cell RNA sequencing, we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct processes, e.g., proliferation, phagocytosis, metabolic homeostasis, and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation, which triggers the differentiation of a novel hemocyte type, the lamellocyte. This first molecular atlas of hemocytes provides insights and paves the way to study the biology of theDrosophilaimmune cells in physiological and pathological conditions. LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Lerner, Zita AU - Magyar, Lilla Brigitta AU - Soós, Bálint AU - Tóth, Renáta AU - Kristó, Ildikó AU - Vilmos, Péter AU - Juhász, Gábor AU - Kovács, Attila Lajos AU - Hegedűs, Zoltán AU - Sensen, Christoph W. AU - Kurucz, Judit Éva AU - Andó, István TI - Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid Zaprionus indianus JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 12 PY - 2020 IS - 3 SP - 257 EP - 272 PG - 16 SN - 1662-811X DO - 10.1159/000502646 UR - https://m2.mtmt.hu/api/publication/30819399 ID - 30819399 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Grawe, Ferdinand AU - Uhlirova, Mirka TI - Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 PG - 27 SN - 2050-084X DO - 10.7554/eLife.57297 UR - https://m2.mtmt.hu/api/publication/31686151 ID - 31686151 N1 - Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany Molecular Cell Biology, Institute I for Anatomy, University of Cologne Medical School, Cologne, Germany Export Date: 8 March 2021 Correspondence Address: Csordás, G.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: cgabor@uni-koeln.de Correspondence Address: Uhlirova, M.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: mirka.uhlirova@uni-koeln.de Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany Molecular Cell Biology, Institute I for Anatomy, University of Cologne Medical School, Cologne, Germany Cited By :1 Export Date: 10 May 2021 Correspondence Address: Csordás, G.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: cgabor@uni-koeln.de Correspondence Address: Uhlirova, M.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: mirka.uhlirova@uni-koeln.de AB - Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation. The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood. Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments. We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment. We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association. These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila LA - English DB - MTMT ER - TY - JOUR AU - Lan, Wenwen AU - Liu, Sumin AU - Zhao, Long AU - Su, Ying TI - Regulation ofDrosophilaHematopoiesis in Lymph Gland: From a Developmental Signaling Point of View JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 15 PG - 15 SN - 1661-6596 DO - 10.3390/ijms21155246 UR - https://m2.mtmt.hu/api/publication/31686813 ID - 31686813 N1 - Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003, China College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China Fisheries College, Ocean University of China, Qingdao, 266003, China Cited By :1 Export Date: 19 January 2022 Correspondence Address: Zhao, L.; Institute of Evolution & Marine Biodiversity, China; email: zhaolong@ouc.edu.cn Correspondence Address: Zhao, L.; Fisheries College, China; email: zhaolong@ouc.edu.cn Correspondence Address: Su, Y.; Institute of Evolution & Marine Biodiversity, China; email: suying@ouc.edu.cn Correspondence Address: Su, Y.; College of Marine Life Sciences, China; email: suying@ouc.edu.cn AB - TheDrosophilahematopoietic system is becoming increasingly attractive for its simple blood cell lineage and its developmental and functional parallels with the vertebrate system. As the dedicated organ forDrosophilalarval hematopoiesis, the lymph gland harbors both multipotent stem-like progenitor cells and differentiated blood cells. The balance between progenitor maintenance and differentiation in the lymph gland must be precisely and tightly controlled. Multiple developmental signaling pathways, such as Notch, Hedgehog, and Wnt/Wingless, have been demonstrated to regulate the hematopoietic processes in the lymph gland. Focusing on blood cell maintenance and differentiation, this article summarizes the functions of several classic developmental signaling pathways for lymph gland growth and patterning, highlighting the important roles of developmental signaling during lymph gland development as well asDrosophilalarval hematopoiesis. LA - English DB - MTMT ER - TY - JOUR AU - Leitão, A.B. AU - Arunkumar, R. AU - Day, J.P. AU - Geldman, E.M. AU - Morin-Poulard, I. AU - Crozatier, M. AU - Jiggins, F.M. TI - Constitutive activation of cellular immunity underlies the evolution of resistance to infection in drosophila JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 SP - 1 EP - 24 PG - 24 SN - 2050-084X DO - 10.7554/ELIFE.59095 UR - https://m2.mtmt.hu/api/publication/33777424 ID - 33777424 N1 - Department of Genetics, University of Cambridge, Cambridge, United Kingdom Centre de Biologie du Développement, Centre de Biologie Intégrative, University Paul Sabatier, Toulouse, France Cited By :11 Export Date: 27 April 2023 Correspondence Address: Leitão, A.B.; Department of Genetics, United Kingdom; email: ac2016@cam.ac.uk Correspondence Address: Jiggins, F.M.email: fmj1001@cam.ac.uk AB - Organisms rely on inducible and constitutive immune defences to combat infection. Constitutive immunity enables a rapid response to infection but may carry a cost for uninfected individuals, leading to the prediction that it will be favoured when infection rates are high. When we exposed populations of Drosophila melanogaster to intense parasitism by the parasitoid wasp Leptopilina boulardi, they evolved resistance by developing a more reactive cellular immune response. Using single-cell RNA sequencing, we found that immune-inducible genes had become constitutively upregulated. This was the result of resistant larvae differentiating precursors of specialized immune cells called lamellocytes that were previously only produced after infection. Therefore, populations evolved resistance by genetically hard-wiring the first steps of an induced immune response to become constitutive. © 2020, eLife Sciences Publications Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Luo, Fangzhou AU - Yu, Shichao AU - Jin, Li Hua TI - The Posterior Signaling Center Is an Important Microenvironment for Homeostasis of the Drosophila Lymph Gland JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 8 PY - 2020 PG - 15 SN - 2296-634X DO - 10.3389/fcell.2020.00382 UR - https://m2.mtmt.hu/api/publication/31469409 ID - 31469409 N1 - Cited By :3 Export Date: 19 January 2022 Correspondence Address: Jin, L.H.; Department of Genetics, China; email: lhjin2000@hotmail.com AB - Hematopoiesis is a necessary process for development and immune defense in Drosophila from the embryonic period to adulthood. There are two main stages in this process: the first stage occurs in the head mesoderm during the embryonic stage, and the second occurs in a specialized hematopoietic organ along the dorsal vessel, the lymph gland, during the larval stage. The lymph gland consists of paired lobes, each of which has distinct regions: the cortical zone (CZ), which contains mature hemocytes; the medullary zone (MZ), which contains hematopoietic progenitors; and the posterior signaling center (PSC), which specifically expresses the early B-cell factor (EBF) transcription factor Collier (Col) and the HOX factor Antennapedia (Antp) to form a microenvironment similar to that of the mammalian bone marrow hematopoietic stem cell niche. The PSC plays a key role in regulating hematopoietic progenitor differentiation. Moreover, the PSC contributes to the cellular immune response to wasp parasitism triggered by elevated ROS levels. Two recent studies have revealed that hematopoietic progenitor maintenance is directly regulated by Col expressed in the MZ and is independent of the PSC, challenging the traditional model. In this review, we summarize the regulatory networks of PSC cell proliferation, the controversy regarding PSC-mediated regulation of hematopoietic progenitor differentiation, and the wasp egg infection response. In addition, we discuss why the PSC is an ideal model for investigating mammalian hematopoietic stem cell niches and leukemia. LA - English DB - MTMT ER - TY - JOUR AU - Madhwal, S. AU - Shin, M. AU - Kapoor, A. AU - Goyal, M. AU - Joshi, M.K. AU - Rehman, P.M.U. AU - Gor, K. AU - Shim, J. AU - Mukherjee, T. TI - Metabolic control of cellular immune-competency by odors in drosophila JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 SP - 1 EP - 93 PG - 93 SN - 2050-084X DO - 10.7554/ELIFE.60376 UR - https://m2.mtmt.hu/api/publication/31868549 ID - 31868549 N1 - Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, Bangalore, 560065, India Department of Life Science, College of Natural Science, Hanyang University, Seoul, 04763, South Korea Vellore Institute of Technology, Katpadi Road, Vellore, Tamil Nadu 632014, India Research Institute for Natural Science, Hanyang University, Seoul, 04763, South Korea Manipal Academy of Higher Education, Manipal, Karnataka 576104, India The University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka 560064, India Export Date: 12 February 2021 Correspondence Address: Shim, J.; Department of Life Science, South Korea; email: jshim@hanyang.ac.kr Correspondence Address: Mukherjee, T.; Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, India; email: tinam@instem.res.in Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, Bangalore, 560065, India Department of Life Science, College of Natural Science, Hanyang University, Seoul, 04763, South Korea Vellore Institute of Technology, Katpadi Road, Vellore, Tamil Nadu 632014, India Research Institute for Natural Science, Hanyang University, Seoul, 04763, South Korea Manipal Academy of Higher Education, Manipal, Karnataka 576104, India The University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka 560064, India Export Date: 14 February 2021 Correspondence Address: Shim, J.; Department of Life Science, South Korea; email: jshim@hanyang.ac.kr Correspondence Address: Mukherjee, T.; Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, India; email: tinam@instem.res.in LA - English DB - MTMT ER - TY - JOUR AU - Nunes, Catarina AU - Sucena, Elio AU - Koyama, Takashi TI - Endocrine regulation of immunity in insects JF - FEBS JOURNAL J2 - FEBS J PY - 2020 PG - 20 SN - 1742-464X DO - 10.1111/febs.15581 UR - https://m2.mtmt.hu/api/publication/31686969 ID - 31686969 N1 - Cited By :7 Export Date: 19 January 2022 CODEN: FJEOA Correspondence Address: Nunes, C.; Instituto Gulbenkian de CiênciaPortugal; email: cdnunes@igc.gulbenkian.pt Correspondence Address: Sucena, É.; Instituto Gulbenkian de CiênciaPortugal; email: esucena@igc.gulbenkian.pt Correspondence Address: Koyama, T.; Department of Biology, Denmark; email: takashi.koyama@bio.ku.dk AB - Organisms have constant contact with potentially harmful agents that can compromise their fitness. However, most of the times these agents fail to cause serious disease by virtue of the rapid and efficient immune responses elicited in the host that can range from behavioural adaptations to immune system triggering. The immune system of insects does not comprise the adaptive arm, making it less complex than that of vertebrates, but key aspects of the activation and regulation of innate immunity are conserved across different phyla. This is the case for the hormonal regulation of immunity as a part of the broad organismal responses to external conditions under different internal states. In insects, depending on the physiological circumstances, distinct hormones either enhance or suppress the immune response integrating individual (and often collective) responses physiologically and behaviourally. In this review, we provide an overview of our current knowledge on the endocrine regulation of immunity in insects, its mechanisms and implications on metabolic adaptation and behaviour. We highlight the importance of this multilayered regulation of immunity in survival and reproduction (fitness) and its dependence on the hormonal integration with other mechanisms and life-history traits. LA - English DB - MTMT ER - TY - JOUR AU - Ramond, Elodie AU - Dudzic, Jan Paul AU - Lemaitre, Bruno TI - Comparative RNA-Seq analyses ofDrosophilaplasmatocytes reveal gene specific signatures in response to clean injury and septic injury JF - PLOS ONE J2 - PLOS ONE VL - 15 PY - 2020 IS - 6 PG - 27 SN - 1932-6203 DO - 10.1371/journal.pone.0235294 UR - https://m2.mtmt.hu/api/publication/31468577 ID - 31468577 N1 - Cited By :9 Export Date: 19 January 2022 CODEN: POLNC Correspondence Address: Ramond, E.; Global Health Institute, Switzerland; email: elodie.ramond@inserm.fr AB - Drosophila melanogaster's blood cells (hemocytes) play essential roles in wound healing and are involved in clearing microbial infections. Here, we report the transcriptional changes of larval plasmatocytes after clean injury or infection with the Gram-negative bacteriumEscherichia colior the Gram-positive bacteriumStaphylococcus aureuscompared to hemocytes recovered from unchallenged larvae via RNA-Sequencing. This study reveals 676 differentially expressed genes (DEGs) in hemocytes from clean injury samples compared to unchallenged samples, and 235 and 184 DEGs inE.coliandS.aureussamples respectively compared to clean injury samples. The clean injury samples showed enriched DEGs for immunity, clotting, cytoskeleton, cell migration, hemocyte differentiation, and indicated a metabolic reprogramming to aerobic glycolysis, a well-defined metabolic adaptation observed in mammalian macrophages. Microbial infections trigger significant transcription of immune genes, with significant differences between theE.coliandS.aureussamples suggesting that hemocytes have the ability to engage various programs upon infection. Collectively, our data bring new insights onDrosophilahemocyte function and open the route to post-genomic functional analysis of the cellular immune response. LA - English DB - MTMT ER - TY - JOUR AU - Ramond, Elodie AU - Petrignani, Bianca AU - Dudzic, Jan Paul AU - Boquete, Jean-Philippe AU - Poidevin, Mickael AU - Kondo, Shu AU - Lemaitre, Bruno TI - The adipokine NimrodB5 regulates peripheral hematopoiesis in Drosophila JF - FEBS JOURNAL J2 - FEBS J PY - 2020 PG - 28 SN - 1742-464X DO - 10.1111/febs.15237 UR - https://m2.mtmt.hu/api/publication/31469236 ID - 31469236 N1 - Global Health Institute, School of Life Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland Centre de Génétique Moléculaire CNRS, Université Pierre et Marie Curie, Gif-sur-Yvette, France Invertebrate Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima, Japan Cited By :8 Export Date: 19 January 2022 CODEN: FJEOA Correspondence Address: Lemaitre, B.; Global Health Institute, Switzerland; email: bruno.lemaitre@epfl.ch AB - In animals, growth is regulated by the complex interplay between paracrine and endocrine signals. When food is scarce, tissues compete for nutrients, leading to critical resource allocation and prioritization. Little is known about how the immune system maturation is coordinated with the growth of other tissues. Here, we describe a signaling mechanism that regulates the number of hemocytes (blood cells) according to the nutritional state of the Drosophila larva. Specifically, we found that a secreted protein, NimB5, is produced in the fat body upon nutrient scarcity downstream of metabolic sensors and ecdysone signaling. NimB5 is then secreted and binds to hemocytes to down-regulate their proliferation and adhesion. Blocking this signaling loop results in conditional lethality when larvae are raised on a poor diet, due to excessive hemocyte numbers and insufficient energy storage. Similar regulatory mechanisms shaping the immune system in response to nutrient availability are likely to be widespread in animals. LA - English DB - MTMT ER - TY - JOUR AU - Tattikota, Sudhir Gopal AU - Cho, Bumsik AU - Liu, Yifang AU - Hu, Yanhui AU - Barrera, Victor AU - Steinbaugh, Michael J. AU - Yoon, Sang-Ho AU - Comjean, Aram AU - Li, Fangge AU - Dervis, Franz AU - Hung, Ruei-Jiun AU - Nam, Jin-Wu AU - Sui, Shannan Ho AU - Shim, Jiwon AU - Perrimon, Norbert TI - A single-cell survey of Drosophila blood JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 PG - 35 SN - 2050-084X DO - 10.7554/eLife.54818 UR - https://m2.mtmt.hu/api/publication/31468416 ID - 31468416 N1 - Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States Department of Life Science, Hanyang University, Seoul, South Korea Harvard TH Chan Bioinformatics Core, Boston, United States Howard Hughes Medical Institute, Boston, United States Cited By :37 Export Date: 19 January 2022 Correspondence Address: Tattikota, S.G.; Department of Genetics, United States; email: sudhir_gt@hms.harvard.edu Correspondence Address: Perrimon, N.; Department of Genetics, United States; email: perrimon@receptor.med.harvard.edu Correspondence Address: Perrimon, N.; Howard Hughes Medical InstituteUnited States; email: perrimon@receptor.med.harvard.edu AB - Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions. LA - English DB - MTMT ER - TY - JOUR AU - Bailetti, Alessandro A. AU - Negron-Pineiro, Lenny J. AU - Dhruva, Vishal AU - Harsh, Sneh AU - Lu, Sean AU - Bosula, Aisha AU - Bach, Erika A. TI - Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity JF - DISEASE MODELS & MECHANISMS J2 - DIS MODEL MECH VL - 12 PY - 2019 IS - 5 PG - 15 SN - 1754-8403 DO - 10.1242/dmm.038679 UR - https://m2.mtmt.hu/api/publication/30901165 ID - 30901165 N1 - Department of Biochemistry and Molecular Pharmacology, New York University, School of Medicine, New York, NY 10016, United States Helen L. and Martin S. Kimmel Center for Stem Cell Biology, New York University, School of Medicine, New York, NY 10016, United States Program in Epithelial Biology, Department of Dermatology, Stanford University, School of Medicine, Stanford, CA 94305, United States Cited By :5 Export Date: 19 January 2022 Correspondence Address: Bach, E.A.; Department of Biochemistry and Molecular Pharmacology, United States; email: erika.bach@nyu.edu AB - Myeloproliferative neoplasms (MPNs) are clonal hematopoietic disorders that cause excessive production of myeloid cells. Most MPN patients have a point mutation in JAK2 (JAK2(V617F)), which encodes a dominant-active kinase that constitutively triggers JAK/STAT signaling. In Drosophila, this pathway is simplified, with a singleJAK, Hopscotch (Hop), and a single STAT transcription factor, Stat92E. The hop(Tumorous-lethal) [hop(Tum)] allele encodes a dominant-active kinase that induces sustained Stat92E activation. Like MPN patients, hop(Tum) mutants have significantly more myeloid cells, which form invasive tumors. Through an unbiased genetic screen, we found that heterozygosity for Enhancer of Polycomb [E(Pc)], a component of the Tip60 lysine acetyltransferase complex (also known as KAT5 in humans), significantly increased tumor burden in hopTum animals. Hematopoietic depletion of E(Pc) or other Tip60 components in an otherwise wild-type background also induced blood cell tumors. The E(Pc) tumor phenotype was dependent on JAK/STAT activity, as concomitant depletion of hop or Stat92E inhibited tumor formation. Stat92E target genes were significantly upregulated in E(Pc)-mutant myeloid cells, indicating that loss of E(Pc) activates JAK STAT signaling. Neither the hop nor Stat92E gene was upregulated upon hematopoietic E(Pc) depletion, suggesting that the regulation of the JAK STAT pathway by E(Pc) is dependent on substrates other than histones. Indeed, E(Pc) depletion significantly increased expression of Hop protein in myeloid cells. This study indicates that E(Pc) works as a tumor suppressor by attenuating Hop protein expression and ultimately JAK STAT signaling. Since loss-of-function mutations in the human homologs of E(Pc) and Tip60 are frequently observed in cancer, our work could lead to new treatments for MPN patients.This article has an associated First Person interview with the first author of the paper. LA - English DB - MTMT ER - TY - JOUR AU - Banerjee, Utpal AU - Girard, Juliet R. AU - Goins, Lauren M. AU - Spratford, Carrie M. TI - Drosophila as a Genetic Model for Hematopoiesis JF - GENETICS J2 - GENETICS VL - 211 PY - 2019 IS - 2 SP - 367 EP - 417 PG - 51 SN - 0016-6731 DO - 10.1534/genetics.118.300223 UR - https://m2.mtmt.hu/api/publication/30510237 ID - 30510237 N1 - Export Date: 13 May 2019 CODEN: GENTA Correspondence Address: Banerjee, U.; University of California, 610 Charles E Young Drive East, United States; email: banerjee@mbi.ucla.edu AB - In this FlyBook chapter, we present a survey of the current literature on the development of the hematopoietic system in Drosophila. The Drosophila blood system consists entirely of cells that function in innate immunity, tissue integrity, wound healing, and various forms of stress response, and are therefore functionally similar to myeloid cells in mammals. The primary cell types are specialized for phagocytic, melanization, and encapsulation functions. As in mammalian systems, multiple sites of hematopoiesis are evident in Drosophila and the mechanisms involved in this process employ many of the same molecular strategies that exemplify blood development in humans. Drosophila blood progenitors respond to internal and external stress by coopting developmental pathways that involve both local and systemic signals. An important goal of these Drosophila studies is to develop the tools and mechanisms critical to further our understanding of human hematopoiesis during homeostasis and dysfunction. LA - English DB - MTMT ER - TY - JOUR AU - Kim-Jo, Chami AU - Gatti, Jean-Luc AU - Poirie, Marylene TI - Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process JF - FRONTIERS IN PHYSIOLOGY J2 - FRONT PHYSIOL VL - 10 PY - 2019 PG - 8 SN - 1664-042X DO - 10.3389/fphys.2019.00603 UR - https://m2.mtmt.hu/api/publication/30907275 ID - 30907275 N1 - Cited By :19 Export Date: 19 January 2022 Correspondence Address: Poirié, M.; INRA, France; email: marylene.poirie@univ-cotedazur.fr AB - Host-parasitoid interactions are among the most studied interactions between invertebrates because of their fundamental interest - the evolution of original traits in parasitoids - and applied, parasitoids being widely used in biological control. Immunity, and in particular cellular immunity, is central in these interactions, the host encapsulation response being specific for large foreign bodies such as parasitoid eggs. Although already well studied in this species, recent data on Drosophila melanogaster have unquestionably improved knowledge of invertebrate cellular immunity. At the same time, the venomics of parasitoids has expanded, notably those of Drosophila. Here, we summarize and discuss these advances, with a focus on an emerging "time-dependent" view of interactions outcome at the intra- and interspecific level. We also present issues still in debate and prospects for study. Data on the Drosophila-parasitoid model paves the way to new concepts in insect immunity as well as parasitoid wasp strategies to overcome it. LA - English DB - MTMT ER - TY - JOUR AU - Melcarne, Claudia AU - Ramond, Elodie AU - Dudzic, Jan AU - Bretscher, Andrew AU - Kurucz, Judit Éva AU - Andó, István AU - Lemaitre, Bruno TI - Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster JF - FEBS JOURNAL J2 - FEBS J VL - 286 PY - 2019 IS - 14 SP - 2670 EP - 2691 PG - 22 SN - 1742-464X DO - 10.1111/febs.14857 UR - https://m2.mtmt.hu/api/publication/30641961 ID - 30641961 N1 - Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary Cited By :13 Export Date: 19 January 2022 CODEN: FJEOA Correspondence Address: Melcarne, C.; Global Health Institute, Switzerland; email: claudia.melcarne@epfl.ch LA - English DB - MTMT ER - TY - CHAP AU - Odenthal, Johanna AU - Brinkkoetter, Paul Thomas ED - Weimbs, Thomas TI - Drosophila melanogaster and its nephrocytes: A versatile model for glomerular research T2 - Methods in Kidney Cell Biology - Part B PB - Elsevier Academic Press CY - San Diego (CA) SN - 9780128203361 T3 - Methods in Cell Biology, ISSN 0091-679X ; 154. PY - 2019 SP - 217 EP - + PG - 8 DO - 10.1016/bs.mcb.2019.03.011 UR - https://m2.mtmt.hu/api/publication/31568110 ID - 31568110 N1 - Cited By :2 Export Date: 19 January 2022 Correspondence Address: Brinkkoetter, P.T.; Department II of Internal Medicine and Center for Molecular Medicine Cologne, Germany; email: paul.brinkkoetter@uk-koeln.de AB - Glomerular disorders are a predominant cause of chronic kidney diseases and end-stage renal failure. Especially podocytes, epithelial cells which represent the outermost part of the filtration barrier, are affected by disease and experience a gradual loss of function. Despite recent advances in identifying potential pathways underlying podocyte injury, treatment remains challenging. It is therefore desirable to employ suitable model organisms in order to study glomerular disease and elucidate affected pathways. Due to its diverse ways of genetic manipulation and high genomic conservation, Drosophila melanogaster is a powerful model organism for biomedical research. The fly was recently used to assess podocytopathies by exploiting the nephrocyte system. Nephrocytes are spherical cells within the body cavity of the fly responsible for detoxification and clearance of unwanted substances. More importantly, they share many characteristics with mammalian podocytes. Here, we summarize how to use Drosophila as a model organism for podocyte research. We discuss examples of techniques that can be used to genetically manipulate nephrocytes and provide protocols for nephrocyte isolation and for morphological as well as functional analysis. LA - English DB - MTMT ER - TY - JOUR AU - Roddie, Hannah Grace AU - Armitage, Emma Louise AU - Coates, Jonathon Alexis AU - Johnston, Simon Andrew AU - Evans, Iwan Robert TI - Simu-dependent clearance of dying cells regulates macrophage function and inflammation resolution JF - PLOS BIOLOGY J2 - PLOS BIOL VL - 17 PY - 2019 IS - 5 PG - 34 SN - 1544-9173 DO - 10.1371/journal.pbio.2006741 UR - https://m2.mtmt.hu/api/publication/30907595 ID - 30907595 N1 - Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, United Kingdom The Bateson Centre, University of Sheffield, Sheffield, United Kingdom Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom Export Date: 31 January 2020 CODEN: PBLIB AB - Macrophages encounter and clear apoptotic cells during normal development and homeostasis, including at numerous sites of pathology. Clearance of apoptotic cells has been intensively studied, but the effects of macrophage-apoptotic cell interactions on macrophage behaviour are poorly understood. Using Drosophila embryos, we have exploited the ease of manipulating cell death and apoptotic cell clearance in this model to identify that the loss of the apoptotic cell clearance receptor Six-microns-under (Simu) leads to perturbation of macrophage migration and inflammatory responses via pathological levels of apoptotic cells. Removal of apoptosis ameliorates these phenotypes, while acute induction of apoptosis phenocopies these defects and reveals that phagocytosis of apoptotic cells is not necessary for their anti-inflammatory action. Furthermore, Simu is necessary for clearance of necrotic debris and retention of macrophages at wounds. Thus, Simu is a general detector of damaged self and represents a novel molecular player regulating macrophages during resolution of inflammation. LA - English DB - MTMT ER - TY - JOUR AU - Varga, Gergely István AU - Csordás, Gábor AU - Cinege, Gyöngyi Ilona AU - Jankovics, Ferenc AU - Sinka, Rita AU - Kurucz, Judit Éva AU - Andó, István AU - Honti, Viktor TI - Headcase is a Repressor of Lamellocyte Fate in Drosophila melanogaster JF - GENES J2 - GENES-BASEL VL - 10 PY - 2019 IS - 3 PG - 17 SN - 2073-4425 DO - 10.3390/genes10030173 UR - https://m2.mtmt.hu/api/publication/30585796 ID - 30585796 N1 - Laboratory of Immunology, Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Laboratory of Drosophila Germ Cell Differentiation, Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Department of Genetics, Faculty of Science and Informatics, University of Szeged, Szeged, 6726, Hungary Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, 50931, Germany Cited By :3 Export Date: 19 January 2022 Correspondence Address: Andó, I.; Laboratory of Immunology, Hungary; email: ando.istvan@brc.mta.hu AB - Due to the evolutionary conservation of the regulation of hematopoiesis, Drosophila provides an excellent model organism to study blood cell differentiation and hematopoietic stem cell (HSC) maintenance. The larvae of Drosophila melanogaster respond to immune induction with the production of special effector blood cells, the lamellocytes, which encapsulate and subsequently kill the invader. Lamellocytes differentiate as a result of a concerted action of all three hematopoietic compartments of the larva: the lymph gland, the circulating hemocytes, and the sessile tissue. Within the lymph gland, the communication of the functional zones, the maintenance of HSC fate, and the differentiation of effector blood cells are regulated by a complex network of signaling pathways. Applying gene conversion, mutational analysis, and a candidate based genetic interaction screen, we investigated the role of Headcase (Hdc), the homolog of the tumor suppressor HECA in the hematopoiesis of Drosophila. We found that naive loss-of-function hdc mutant larvae produce lamellocytes, showing that Hdc has a repressive role in effector blood cell differentiation. We demonstrate that hdc genetically interacts with the Hedgehog and the Decapentaplegic pathways in the hematopoietic niche of the lymph gland. By adding further details to the model of blood cell fate regulation in the lymph gland of the larva, our findings contribute to the better understanding of HSC maintenance. LA - English DB - MTMT ER - TY - JOUR AU - Baldeosingh, Rajkumar AU - Gao, Hongjuan AU - Wu, Xiaorong AU - Fossett, Nancy TI - Hedgehog signaling from the Posterior Signaling Center maintains U-shaped expression and a prohemocyte population in Drosophila JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 441 PY - 2018 IS - 1 SP - 132 EP - 145 PG - 14 SN - 0012-1606 DO - 10.1016/j.ydbio.2018.06.020 UR - https://m2.mtmt.hu/api/publication/30510582 ID - 30510582 N1 - Graduate Program in Life Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, United States Center for Vascular and Inflammatory Diseases and the Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201, United States The Wilmer Eye Institute, Johns Hopkins University School of Medicine, 400N. Broadway M002 P, Baltimore, MD 21231, United States Cited By :19 Export Date: 19 January 2022 CODEN: DEBIA Correspondence Address: Fossett, N.; University of Maryland, 800 W. Baltimore Street, Room 215, United States; email: nfossett@som.umaryland.edu AB - Hematopoietic progenitor choice between multipotency and differentiation is tightly regulated by intrinsic factors and extrinsic signals from the surrounding microenvironment. The Drosophila melanogaster hematopoietic lymph gland has emerged as a powerful tool to investigate mechanisms that regulate hematopoietic progenitor choice in vivo. The lymph gland contains progenitor cells, which share key characteristics with mammalian hematopoietic progenitors such as quiescence, multipotency and niche dependence. The lymph gland is zonally arranged, with progenitors located in medullary zone, differentiating cells in the cortical zone, and the stem cell niche or Posterior Signaling Center (PSC) residing at the base of the medullary zone (MZ). This arrangement facilitates investigations into how signaling from the microenvironment controls progenitor choice. The Drosophila Friend of GATA transcriptional regulator, U-shaped, is a conserved hematopoietic regulator. To identify additional novel intrinsic and extrinsic regulators that interface with U-shaped to control hematopoiesis, we conducted an in vivo screen for factors that genetically interact with u-shaped. Smoothened, a downstream effector of Hedgehog signaling, was one of the factors identified in the screen. Here we report our studies that characterized the relationship between Smoothened and U-shaped. We showed that the PSC and Hedgehog signaling are required for U-shaped expression and that U-shaped is an important intrinsic progenitor regulator. These observations identify a potential link between the progenitor regulatory machinery and extrinsic signals from the PSC. Furthermore, we showed that both Hedgehog signaling and the PSC are required to maintain a subpopulation of progenitors. This led to a delineation of PSC-dependent versus PSC-independent progenitors and provided further evidence that the MZ progenitor population is heterogeneous. Overall, we have identified a connection between a conserved hematopoietic master regulator and a putative stem cell niche, which adds to our understanding of how signals from the microenvironment regulate progenitor multipotency. LA - English DB - MTMT ER - TY - JOUR AU - Bazzi, Wael AU - Cattenoz, Pierre B AU - Delaporte, Claude AU - Dasari, Vasanthi AU - Sakr, Rosy AU - Yuasa, Yoshihiro AU - Giangrande, Angela TI - Embryonic hematopoiesis modulates the inflammatory response and larval hematopoiesis in Drosophila JF - ELIFE J2 - ELIFE VL - 7 PY - 2018 PG - 30 SN - 2050-084X DO - 10.7554/eLife.34890.001 UR - https://m2.mtmt.hu/api/publication/27602855 ID - 27602855 AB - Recent lineage tracing analyses have significantly improved our understanding of immune system development and highlighted the importance of the different hematopoietic waves. The current challenge is to understand whether these waves interact and whether this affects the function of the immune system. Here we report a molecular pathway regulating the immune response and involving the communication between embryonic and larval hematopoietic waves in Drosophila. Down-regulating the transcription factor Gcm specific to embryonic hematopoiesis enhances the larval phenotypes induced by over-expressing the pro-inflammatory Jak/Stat pathway or by wasp infestation. Gcm works by modulating the transduction of the Upd cytokines to the site of larval hematopoiesis and hence the response to chronic Jak/Stat overexpression and acute wasp infestation immune challenges. Thus, homeostatic interactions control the function of the immune system in physiology and pathology. Our data also indicate that a transiently expressed developmental pathway has a long-lasting effect on the immune response. © Bazzi et al. LA - English DB - MTMT ER - TY - JOUR AU - Boulet, Manon AU - Miller, Marion AU - Vandel, Laurence AU - Waltzer, Lucas TI - From Drosophila Blood Cells to Human Leukemia JF - ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY J2 - ADV EXP MED BIOL VL - 1076 PY - 2018 SP - 195 EP - 214 PG - 20 SN - 0065-2598 DO - 10.1007/978-981-13-0529-0_11 UR - https://m2.mtmt.hu/api/publication/30510499 ID - 30510499 N1 - Cited By :8 Export Date: 15 February 2024 CODEN: AEMBA Correspondence Address: Waltzer, L.; Université Clermont Auvergne, France; email: lucas.waltzer@uca.fr AB - The hematopoietic system plays a critical role in establishing the proper response against invading pathogens or in removing cancerous cells. Furthermore, deregulations of the hematopoietic differentiation program are at the origin of numerous diseases including leukemia. Importantly, many aspects of blood cell development have been conserved from human to Drosophila. Hence, Drosophila has emerged as a potent genetic model to study blood cell development and leukemia in vivo. In this chapter, we give a brief overview of the Drosophila hematopoietic system, and we provide a protocol for the dissection and the immunostaining of the larval lymph gland, the most studied hematopoietic organ in Drosophila. We then focus on the various paradigms that have been used in fly to investigate how conserved genes implicated in leukemogenesis control blood cell development. Specific examples of Drosophila models for leukemia are presented, with particular attention to the most translational ones. Finally, we discuss some limitations and potential improvements of Drosophila models for studying blood cell cancer. LA - English DB - MTMT ER - TY - JOUR AU - Hao, Yangguang AU - Yu, Shichao AU - Luo, Fangzhou AU - Jin, Li Hua TI - Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila JF - CELL COMMUNICATION AND SIGNALING J2 - CELL COMM SIGN VL - 16 PY - 2018 PG - 20 SN - 1478-811X DO - 10.1186/s12964-018-0305-3 UR - https://m2.mtmt.hu/api/publication/30510502 ID - 30510502 N1 - Department of Genetics, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China Department of Translational Medicine Research Center, Shenyang Medical College, Shenyang, 110034, China Cited By :7 Export Date: 19 January 2022 Correspondence Address: Jin, L.H.; Department of Genetics, China; email: lhjin@nefu.edu.cn AB - Background: The regulatory mechanisms of hematopoiesis and cellular immunity show a high degree of similarity between insects and mammals, and Drosophila has become a good model for investigating cellular immune responses. Jumeau (Jumu) is a member of the winged-helix/forkhead (FKH) transcription factor family and is required for Drosophila development. Adult jumu mutant flies show defective hemocyte phagocytosis and a weaker defense capability against pathogen infection. Here, we further investigated the role of jumu in the regulation of larval hemocyte development and phagocytosis. LA - English DB - MTMT ER - TY - JOUR AU - Anderson, Abigail M AU - Bailetti, Alessandro A AU - Rodkin, Elizabeth AU - De, Atish AU - Bach, Erika A TI - A Genetic Screen Reveals an Unexpected Role for Yorkie Signaling in JAK/STAT-Dependent Hematopoietic Malignancies in Drosophila melanogaster JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 7 PY - 2017 IS - 8 SP - 2427 EP - 2438 PG - 12 SN - 2160-1836 DO - 10.1534/g3.117.044172 UR - https://m2.mtmt.hu/api/publication/26891561 ID - 26891561 N1 - Megjegyzés-26822334 OA gold LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Zsámboki, János AU - Vidal-Quadras, M AU - Uv, A AU - Csordás, Gábor AU - Honti, Viktor AU - Gábor, Erika AU - Hegedűs, Zoltán AU - Varga, Gergely István AU - Kovács, Attila Lajos AU - Juhász, Gábor AU - Williams, MJ AU - Andó, István AU - Kurucz, Judit Éva TI - Genes encoding cuticular proteins are components of the Nimrod gene cluster in Drosophila. JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 87 PY - 2017 SP - 45 EP - 54 PG - 10 SN - 0965-1748 DO - 10.1016/j.ibmb.2017.06.006 UR - https://m2.mtmt.hu/api/publication/3249679 ID - 3249679 N1 - Immunology Unit, Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, P.O.Box 521, Szeged, H-6701, Hungary Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SE-40530, Sweden Laboratory of Bioinformatics, Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, P.O.Box 521, Szeged, H-6701, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Department of Neuroscience, Functional Pharmacology, Uppsala University, Sweden Institution För Neurovetenskap BMC, Husargatan 3, Box 593 751 24, Uppsala, Sweden Cited By :10 Export Date: 7 November 2023 CODEN: IBMBE Correspondence Address: Kurucz, É.; Immunology Unit, P.O.Box 521, Hungary; email: kurucz.eva@brc.mta.hu AB - The Nimrod gene cluster, located on the second chromosome of Drosophila melanogaster, is the largest synthenic unit of the Drosophila genome. Nimrod genes show blood cell specific expression and code for phagocytosis receptors that play a major role in fruit fly innate immune functions. We previously identified three homologous genes (vajk-1, vajk-2 and vajk-3) located within the Nimrod cluster, which are unrelated to the Nimrod genes, but are homologous to a fourth gene (vajk-4) located outside the cluster. Here we show that, unlike the Nimrod candidates, the Vajk proteins are expressed in cuticular structures of the late embryo and the late pupa, indicating that they contribute to cuticular barrier functions. LA - English DB - MTMT ER - TY - JOUR AU - Del Signore, Steven J AU - Biber, Sarah A AU - Lehmann, Katherine S AU - Heimler, Stephanie R AU - Rosenfeld, Benjamin H AU - Eskin, Tania L AU - Sweeney, Sean T AU - Rodal, Avital A TI - dOCRL maintains immune cell quiescence by regulating endosomal traffic JF - PLOS GENETICS J2 - PLOS GENET VL - 13 PY - 2017 IS - 10 PG - 29 SN - 1553-7390 DO - 10.1371/journal.pgen.1007052 UR - https://m2.mtmt.hu/api/publication/27055182 ID - 27055182 N1 - Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA, United States Department of Biology, University of York, York, United Kingdom Cited By :6 Export Date: 4 May 2021 Correspondence Address: Rodal, A.A.; Rosenstiel Basic Medical Sciences Research Center, United States; email: arodal@brandeis.edu LA - English DB - MTMT ER - TY - CHAP AU - El, Chamy L. AU - Matt, N. AU - Reichhart, J.-M. ED - Gordon, Siamon TI - Advances in myeloid-like cell origins and functions in the model organism Drosophila melanogaster T2 - Myeloid Cells in Health and Disease PB - ASM Press CY - Washington DC SN - 9781683670667 PY - 2017 SP - 59 EP - 77 PG - 19 DO - 10.1128/9781555819194.ch5 UR - https://m2.mtmt.hu/api/publication/34589227 ID - 34589227 N1 - Laboratoire de Génétique de la Drosophile et Virulence Microbienne, UR. EGFEM, Faculté des Sciences, Université Saint-Joseph de Beyrouth, B.P. 17-5208 Mar Mikhaël, Beyrouth, 1104 2020, Lebanon Université de Strasbourg, UPR 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg Cedex, 67084, France Export Date: 15 February 2024 AB - Innate immunity shields all metazoans against infections. Its main features, including sensing, signaling, and effector mechanisms, are conserved from invertebrates to vertebrates. The hallmark of innate immunity is its reliance on a limited set of non-clonally-distributed receptors, which detect signature molecules of microbial origin and activate subsequent effector mechanisms. This concept, coined by Charles Janeway in 1989 as the self-versus-microbial-nonself discrimination system, has opened a large field of research for the so-called pattern recognition receptors (PRRs) and their cognate microbial elicitors, the pathogen-associated molecular patterns (1). Drosophila has rapidly emerged as a particularly suitable model organism for this research. Indeed, like all invertebrates, Drosophila exclusively relies on an innate immune system, which fends off infections in highly contaminated environments. Most importantly, Drosophila has benefited from more than a century of laboratory-use experience, yielding a wide array of molecular and genetic tools. Investigations on the defense reactions in flies rapidly provided valuable insights into the evolutionary conservation between insects and mammals, including humans, of the signal transduction pathways that control the innate immune system (2). Most prominent is the seminal finding in 1996 of the chief role of the Toll signaling pathway in the control of fungal infections in Drosophila (3). This study paved the way for the identification of the first mammalian PRR, Toll-like receptor 4 (the launching member of the TLR family), and the understanding of the innate immune system’s molecular mechanisms for sensing, signaling, and activation of adaptive immunity (4 - 6). Following more than 2 decades of in-depth analysis exploiting several infection models combined with genetic and genomic approaches, research on the Drosophila immune system revealed complex interconnected humoral and cellular processes, both of which show striking similarities with those of mammals. In this review, we provide a global view of the Drosophila host defense while drawing particular attention to the role of its monocyte-macrophage-like cells, the plasmatocytes. We provide general insights on the recent advances in Drosophila hematopoiesis and give a comprehensive summary on the so-far identified receptors involved in microbial detection, binding, and the ensuing internalization processes. © 2017 American Society for Microbiology, Washington, DC. LA - English DB - MTMT ER - TY - CHAP AU - El, Chamy L. AU - Matt, N. AU - Reichhart, J.-M. ED - Gordon, Siamon TI - Advances in myeloid-like cell origins and functions in the model organism Drosophila melanogaster T2 - Myeloid Cells in Health and Disease PB - ASM Press CY - Washington DC SN - 9781683670667 PY - 2017 SP - 59 EP - 77 PG - 19 DO - 10.1128/9781555819194.ch5 UR - https://m2.mtmt.hu/api/publication/32006335 ID - 32006335 N1 - Laboratoire de Génétique de la Drosophile et Virulence Microbienne, UR. EGFEM, Faculté des Sciences, Université Saint-Joseph de Beyrouth, B.P. 17-5208 Mar Mikhaël, Beyrouth, 1104 2020, Lebanon Université de Strasbourg, UPR 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg Cedex, 67084, France Export Date: 10 May 2021 AB - Innate immunity shields all metazoans against infections. Its main features, including sensing, signaling, and effector mechanisms, are conserved from invertebrates to vertebrates. The hallmark of innate immunity is its reliance on a limited set of non-clonally-distributed receptors, which detect signature molecules of microbial origin and activate subsequent effector mechanisms. This concept, coined by Charles Janeway in 1989 as the self-versus-microbial-nonself discrimination system, has opened a large field of research for the so-called pattern recognition receptors (PRRs) and their cognate microbial elicitors, the pathogen-associated molecular patterns (1). Drosophila has rapidly emerged as a particularly suitable model organism for this research. Indeed, like all invertebrates, Drosophila exclusively relies on an innate immune system, which fends off infections in highly contaminated environments. Most importantly, Drosophila has benefited from more than a century of laboratory-use experience, yielding a wide array of molecular and genetic tools. Investigations on the defense reactions in flies rapidly provided valuable insights into the evolutionary conservation between insects and mammals, including humans, of the signal transduction pathways that control the innate immune system (2). Most prominent is the seminal finding in 1996 of the chief role of the Toll signaling pathway in the control of fungal infections in Drosophila (3). This study paved the way for the identification of the first mammalian PRR, Toll-like receptor 4 (the launching member of the TLR family), and the understanding of the innate immune system’s molecular mechanisms for sensing, signaling, and activation of adaptive immunity (4 - 6). Following more than 2 decades of in-depth analysis exploiting several infection models combined with genetic and genomic approaches, research on the Drosophila immune system revealed complex interconnected humoral and cellular processes, both of which show striking similarities with those of mammals. In this review, we provide a global view of the Drosophila host defense while drawing particular attention to the role of its monocyte-macrophage-like cells, the plasmatocytes. We provide general insights on the recent advances in Drosophila hematopoiesis and give a comprehensive summary on the so-far identified receptors involved in microbial detection, binding, and the ensuing internalization processes. © 2017 American Society for Microbiology, Washington, DC. LA - English DB - MTMT ER - TY - JOUR AU - El, Chamy Laure AU - Matt, Nicolas AU - Reichhart, Jean-Marc TI - Advances in Myeloid-Like Cell Origins and Functions in the Model Organism Drosophila melanogaster JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 5 PY - 2017 IS - 1 PG - 17 SN - 2165-0497 DO - 10.1128/microbiolspec.MCHD-0038-2016 UR - https://m2.mtmt.hu/api/publication/26536389 ID - 26536389 N1 - Laboratoire de Génétique de la drosophile et virulence microbienne, UR. EGFEM, Faculté des Sciences, Université Saint-Joseph de Beyrouth, B.P. 17-5208, Mar Mikhaël Beyrouth, 1104 2020, Lebanon Université de Strasbourg, UPR 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg Cedex, 67084, France Cited By :3 Export Date: 19 January 2022 Correspondence Address: El Chamy, L.; Laboratoire de Génétique de la drosophile et virulence microbienne, B.P. 17-5208, Lebanon; email: Laure.chamy@usj.edu.lb LA - English DB - MTMT ER - TY - JOUR AU - Louradour, Isabelle AU - Sharma, Anurag AU - Morin-Poulard, Ismael AU - Letourneau, Manon AU - Vincent, Alain AU - Crozatier, Michele AU - Vanzo, Nathalie TI - Reactive oxygen species-dependent Toll/NF-kappa B activation in the Drosophila hematopoietic niche confers resistance to wasp parasitism JF - ELIFE J2 - ELIFE VL - 6 PY - 2017 PG - 22 SN - 2050-084X DO - 10.7554/eLife.25496 UR - https://m2.mtmt.hu/api/publication/27260897 ID - 27260897 N1 - Centre de Biologie du Développement, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France National Institutes of Health, Bethesda, United States Department of Biomedical Sciences, NU Centre for Science Education and Research, Nitte University, Mangalore, India Hubrecht Institute, Utrecht, Netherlands Cited By :35 Export Date: 19 January 2022 Correspondence Address: Crozatier, M.; Centre de Biologie du Développement, France; email: michele.crozatier-borde@univ-tlse3.fr LA - English DB - MTMT ER - TY - JOUR AU - Tokusumi, Tsuyoshi AU - Tokusumi, Yumiko AU - Brahier, Mark S AU - Lam, Victoria AU - Stoller-Conrad, Jessica R AU - Kroeger, Paul T AU - Schulz, Robert A TI - Screening and Analysis of Janelia FlyLight Project Enhancer-Gal4 Strains Identifies Multiple Gene Enhancers Active During Hematopoiesis in Normal and Wasp-Challenged Drosophila Larvae JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 7 PY - 2017 IS - 2 SP - 437 EP - 448 PG - 12 SN - 2160-1836 DO - 10.1534/g3.116.034439 UR - https://m2.mtmt.hu/api/publication/26536387 ID - 26536387 N1 - Cited By :12 Export Date: 19 January 2022 Correspondence Address: Schulz, R.A.; Department of Biological Sciences, 147 Galvin Life Science Building, United States; email: rschulz@nd.edu LA - English DB - MTMT ER - TY - JOUR AU - Anderl, I AU - Vesala, L AU - Ihalainen, TO AU - Vanha-Aho, LM AU - Andó, István AU - Ramet, M AU - Hultmark, D TI - Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection. JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 12 PY - 2016 IS - 7 SP - e1005746 SN - 1553-7366 DO - 10.1371/journal.ppat.1005746 UR - https://m2.mtmt.hu/api/publication/3096913 ID - 3096913 N1 - WoS:hiba:000383366400030 2019-03-03 19:41 első oldal nem egyezik AB - Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail. LA - English DB - MTMT ER - TY - JOUR AU - Bryant, William B AU - Michel, Kristin TI - Anopheles gambiae hemocytes exhibit transient states of activation JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 55 PY - 2016 SP - 119 EP - 129 PG - 11 SN - 0145-305X DO - 10.1016/j.dci.2015.10.020 UR - https://m2.mtmt.hu/api/publication/25771414 ID - 25771414 N1 - Cited By :22 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Michel, K.; Division of Biology, United States; email: kmichel@ksu.edu LA - English DB - MTMT ER - TY - JOUR AU - Dey, Nidhi Sharma AU - Ramesh, Parvathy AU - Chugh, Mayank AU - Mandal, Sudip AU - Mandal, Lolitika TI - Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila JF - ELIFE J2 - ELIFE VL - 5 PY - 2016 PG - 24 SN - 2050-084X DO - 10.7554/eLife.18295 UR - https://m2.mtmt.hu/api/publication/26377269 ID - 26377269 N1 - Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India Center for Plant Molecular Biology, University of Tuebingen, Tuebingen, Germany Cited By :31 Export Date: 19 January 2022 Correspondence Address: Mandal, L.; Developmental Genetics Laboratory, India; email: lolitika@iisermohali.ac.in LA - English DB - MTMT ER - TY - JOUR AU - Forbes-Beadle, Lauren AU - Crossman, Tova AU - Johnson, Travis K AU - Burke, Richard AU - Warr, Coral G AU - Whisstock, James C TI - Development of the Cellular Immune System of Drosophila Requires the Membrane Attack Complex/Perforin-Like Protein Torso-Like JF - GENETICS J2 - GENETICS VL - 204 PY - 2016 IS - 2 SP - 675 EP - U1161 PG - 11 SN - 0016-6731 DO - 10.1534/genetics.115.185462 UR - https://m2.mtmt.hu/api/publication/26203751 ID - 26203751 N1 - Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, VIC 3800, Australia School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia Cited By :8 Export Date: 19 January 2022 CODEN: GENTA Correspondence Address: Warr, C.G.; School of Biological Sciences, Australia; email: coral.warr@monash.edu LA - English DB - MTMT ER - TY - JOUR AU - Hillyer, Julian F TI - Insect immunology and hematopoiesis JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 58 PY - 2016 SP - 102 EP - 118 PG - 17 SN - 0145-305X DO - 10.1016/j.dci.2015.12.006 UR - https://m2.mtmt.hu/api/publication/25771630 ID - 25771630 N1 - Cited By :187 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Hillyer, J.F.; Department of Biological Sciences, VU Station B 35-1634, United States; email: julian.hillyer@vanderbilt.edu LA - English DB - MTMT ER - TY - JOUR AU - Letourneau, Manon AU - Lapraz, Francois AU - Sharma, Anurag AU - Vanzo, Nathalie AU - Waltzer, Lucas AU - Crozatier, Michele TI - Drosophila hematopoiesis under normal conditions and in response to immune stress JF - FEBS LETTERS J2 - FEBS LETT VL - 590 PY - 2016 IS - 22 SP - 4034 EP - 4051 PG - 18 SN - 0014-5793 DO - 10.1002/1873-3468.12327 UR - https://m2.mtmt.hu/api/publication/26377268 ID - 26377268 N1 - Megjegyzés-26322880 N1 Funding details: ANR, Agence Nationale de la Recherche N1 Funding details: ARC, Association pour la Recherche sur le Cancer N1 Funding details: CNRS, Centre National de la Recherche Scientifique N1 Funding details: IFCPAR, Indo-French Centre for the Promotion of Advanced Research N1 Funding text: We thank M. Meister and C. Monod for critical reading of the manuscript. Work in the authors’ laboratory is supported by the CNRS, University Toulouse III, Ministère de la Recherche (ANR « programme blanc »), ARC (Association pour la Recherche sur le Cancer) and CEFIPRA (Centre Franco-Indien pour la Promotion de la Recherche Avancée). ML, FL, AS, NV, LW and MC wrote the manuscript. ML made the figures, LW & MC designed the review. LA - English DB - MTMT ER - TY - JOUR AU - Schmid, Martin R AU - Anderl, Ines AU - Vo, Hoa T M AU - Valanne, Susanna AU - Yang, Hairu AU - Kronhamn, Jesper AU - Ramet, Mika AU - Rusten, Tor Erik AU - Hultmark, Dan TI - Genetic Screen in Drosophila Larvae Links ird1 Function to Toll Signaling in the Fat Body and Hemocyte Motility JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 7 PG - 30 SN - 1932-6203 DO - 10.1371/journal.pone.0159473 UR - https://m2.mtmt.hu/api/publication/26237558 ID - 26237558 N1 - Department of Molecular Biology, Umeå University, Umeå, Sweden BioMediTech, University of Tampere, Tampere, Finland PEDEGO Research Center, Medical Research Center Oulu, University of Oulu, Oulu University Hospital, Oulu, Finland Department of Molecular Cell Biology, Oslo University Hospital, Centre for Cancer Biomedicine, University of Oslo, Oslo, Norway Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden Cited By :6 Export Date: 23 September 2021 CODEN: POLNC LA - English DB - MTMT ER - TY - CHAP AU - Smith, VJ AU - Accorsi, A AU - Malagoli, D ED - Davide, Malagoli TI - Hematopoiesis and hemocytes in pancrustacean and molluscan models T2 - The Evolution of the Immune System: Conservation and Diversification PB - Elsevier Inc. CY - Amsterdam SN - 9780128020135 PB - Elsevier Inc. PY - 2016 SP - 1 EP - 28 PG - 28 DO - 10.1016/B978-0-12-801975-7.00001-3 UR - https://m2.mtmt.hu/api/publication/26674055 ID - 26674055 N1 - Scottish Oceans Institute, School of Biology, University of St Andrews, St Andrews, Fife, United Kingdom Stowers Institute for Medical Research, Kansas City, MO, United States Howard Hughes Medical Institute, Stowers Institute for Medical Research, Kansas City, MO, United States Department of Life Sciences, University of Modena and Reggio Emilia, Biology Building, Modena, Italy Cited By :10 Export Date: 19 January 2022 Correspondence Address: Smith, V.J.; Scottish Oceans Institute, United Kingdom LA - English DB - MTMT ER - TY - JOUR AU - Anderl, Ines AU - Hultmark, Dan TI - New ways to make a blood cell JF - ELIFE J2 - ELIFE VL - 4 PY - 2015 PG - 3 SN - 2050-084X DO - 10.7554/eLife.06877 UR - https://m2.mtmt.hu/api/publication/24796091 ID - 24796091 LA - English DB - MTMT ER - TY - JOUR AU - Bretscher, AJ AU - Honti, Viktor AU - Binggeli, O AU - Burri, O AU - Poidevin, M AU - Kurucz, Judit Éva AU - Zsámboki, János AU - Andó, István AU - Lemaitre, B TI - The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster. JF - BIOLOGY OPEN J2 - BIOL OPEN VL - 4 PY - 2015 IS - 3 SP - 355 EP - 363 PG - 9 SN - 2046-6390 DO - 10.1242/bio.201410595 UR - https://m2.mtmt.hu/api/publication/2853633 ID - 2853633 AB - Eater is an EGF-like repeat transmembrane receptor of the Nimrod family and is expressed in Drosophila hemocytes. Eater was initially identified for its role in phagocytosis of both Gram-positive and Gram-negative bacteria. We have deleted eater and show that it appears to be required for efficient phagocytosis of Gram-positive but not Gram-negative bacteria. However, the most striking phenotype of eater deficient larvae is the near absence of sessile hemocytes, both plasmatocyte and crystal cell types. The eater deletion is the first loss of function mutation identified that causes absence of the sessile hemocyte state. Our study shows that Eater is required cell-autonomously in plasmatocytes for sessility. However, the presence of crystal cells in the sessile compartment requires Eater in plasmatocytes. We also show that eater deficient hemocytes exhibit a cell adhesion defect. Collectively, our data uncovers a new requirement of Eater in enabling hemocyte attachment at the sessile compartment and points to a possible role of Nimrod family members in hemocyte adhesion. LA - English DB - MTMT ER - TY - JOUR AU - Ghosh, S AU - Singh, A AU - Mandal, S AU - Mandal, L TI - Active Hematopoietic Hubs in Drosophila Adults Generate Hemocytes and Contribute to Immune Response JF - DEVELOPMENTAL CELL J2 - DEV CELL VL - 33 PY - 2015 IS - 4 SP - 478 EP - 488 PG - 11 SN - 1534-5807 DO - 10.1016/j.devcel.2015.03.014 UR - https://m2.mtmt.hu/api/publication/24982068 ID - 24982068 N1 - Cited By :78 Export Date: 30 June 2022 CODEN: DCEEB LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Andó, István TI - Innate immunity JF - ACTA BIOLOGICA SZEGEDIENSIS J2 - ACTA BIOL SZEGED VL - 59 PY - 2015 IS - Suppl. 1 SP - 1 EP - 15 PG - 15 SN - 1588-385X UR - https://m2.mtmt.hu/api/publication/2993019 ID - 2993019 N1 - Export Date: 19 January 2022 CODEN: ABSCC Correspondence Address: Andó, I.; Immunology Unit, Hungary; email: ando@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Leitao, Alexandre B AU - Sucena, Elio TI - Drosophila sessile hemocyte clusters are true hematopoietic tissues that regulate larval blood cell differentiation JF - ELIFE J2 - ELIFE VL - 4 PY - 2015 IS - 4 SP - 1 EP - 38 PG - 16 SN - 2050-084X DO - 10.7554/eLife.06166 UR - https://m2.mtmt.hu/api/publication/24797542 ID - 24797542 N1 - Cited By :64 Export Date: 19 January 2022 Correspondence Address: Leitão, A.B.; Instituto Gulbenkian de Ciência, Apartado 14, Portugal; email: aleitao@igc.gulbenkian.pt AB - Virtually all species of coelomate animals contain blood cells that display a division of labor necessary for homeostasis. This functional partition depends upon the balance between proliferation and differentiation mostly accomplished in the hematopoietic organs. In Drosophila melanogaster, the lymph gland produces plasmatocytes and crystal cells that are not released until pupariation. Yet, throughout larval development, both hemocyte types increase in numbers. Mature plasmatocytes can proliferate but it is not known if crystal cell numbers increase by self-renewal or by de novo differentiation. We show that new crystal cells in third instar larvae originate through a Notch-dependent process of plasmatocyte transdifferentiation. This process occurs in the sessile clusters and is contingent upon the integrity of these structures. The existence of this hematopoietic tissue, relying on structure-dependent signaling events to promote blood homeostasis, creates a new paradigm for addressing outstanding questions in Drosophila hematopoiesis and establishing further parallels with vertebrate systems. © 2015, eLife Sciences Publications Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Lerner, Zita AU - Honti, Viktor AU - Csordás, Gábor AU - Zsámboki, János AU - Cinege, Gyöngyi Ilona AU - Párducz, Árpád AU - Lukacsovich, Tamás AU - Kurucz, Judit Éva AU - Andó, István TI - Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 7 PY - 2015 IS - 4 SP - 340 EP - 353 PG - 14 SN - 1662-811X DO - 10.1159/000369618 UR - https://m2.mtmt.hu/api/publication/2853634 ID - 2853634 N1 - Megjegyzés-25258522 Hiányzó Jelleg: 'JOUR\n\nArticle' Admin megjegyzés-25258522 tblcategory: (Category) ('JOUR\n\nArticle') #Jelleg AB - We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in the ananassae subgroup of Drosophilidae. Here, we describe the functional and ultrastructural characteristics of this novel blood cell type as well as its characterization with a set of discriminative immunological markers. MGHs are encapsulating cells that isolate and kill the parasite without melanization. They share some properties with but differ considerably from lamellocytes, the encapsulating cells of Drosophila melanogaster, the broadly used model organism in studies of innate immunity. MGHs are nonproliferative effector cells that are derived from phagocytic cells of the sessile tissue and the circulation, but do not exhibit phagocytic activity. In contrast to lamellocytes, MGHs are gigantic cells with filamentous projections and contain many nuclei, which are the result of the fusion of several cells. Although the structure of lamellocytes and MGHs differ remarkably, their function in the elimination of parasites is similar, which is potentially the result of the convergent evolution of interactions between hosts and parasites in different geographic regions. MGHs are highly motile and share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation. © 2015 S. Karger AG, Basel LA - English DB - MTMT ER - TY - JOUR AU - Neyen, Claudine AU - Binggeli, Olivier AU - Roversi, Pietro AU - Bertin, Lise AU - Sleiman, Maroun Bou AU - Lemaitre, Bruno TI - The Black cells phenotype is caused by a point mutation in the Drosophila pro-phenoloxidase 1 gene that triggers melanization and hematopoietic defects JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 50 PY - 2015 IS - 2 SP - 166 EP - 174 PG - 9 SN - 0145-305X DO - 10.1016/j.dci.2014.12.011 UR - https://m2.mtmt.hu/api/publication/24796089 ID - 24796089 N1 - Global Health Institute, Swiss Federal Institute of Technology, Station 19, Lausanne, CH-1015, Switzerland Department of Biochemistry, University of Oxford, Oxford, X13QU, United Kingdom Cited By :15 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Neyen, C.; EPFL-SV-GHI-UPLEM, Station 19, Switzerland LA - English DB - MTMT ER - TY - JOUR AU - Reimels, Theresa A AU - Pfleger, Cathie M TI - Drosophila Rabex-5 restricts Notch activity in hematopoietic cells and maintains hematopoietic homeostasis JF - JOURNAL OF CELL SCIENCE J2 - J CELL SCI VL - 128 PY - 2015 IS - 24 SP - 4512 EP - 4525 PG - 14 SN - 0021-9533 DO - 10.1242/jcs.174433 UR - https://m2.mtmt.hu/api/publication/25771415 ID - 25771415 N1 - Cited By :5 Export Date: 30 June 2022 CODEN: JNCSA LA - English DB - MTMT ER - TY - JOUR AU - Vanha-aho, LM AU - Anderl, I AU - Vesala, L AU - Hultmark, D AU - Valanne, S AU - Ramet, M TI - Edin Expression in the Fat Body Is Required in the Defense Against Parasitic Wasps in Drosophila melanogaster JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 11 PY - 2015 IS - 5 PG - 18 SN - 1553-7366 DO - 10.1371/journal.ppat.1004895 UR - https://m2.mtmt.hu/api/publication/24975884 ID - 24975884 N1 - Laboratory of Experimental Immunology, BioMediTech, University of Tampere, Tampere, Finland Laboratory of Genetic Immunology, BioMediTech, University of Tampere, Tampere, Finland Department of Molecular Biology, Umeå University, Umeå, Sweden Department of Pediatrics, Tampere University Hospital, Tampere, Finland PEDEGO Research Center, and Medical Research Center Oulu, University of Oulu and Department of Children and Adolescents, Oulu University Hospital, Oulu, Finland Cited By :26 Export Date: 19 January 2022 Correspondence Address: Rämet, M.; Laboratory of Experimental Immunology, Finland; email: mika.ramet@uta.fi LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Varga, Gergely István AU - Honti, Viktor AU - Jankovics, Ferenc AU - Kurucz, Judit Éva AU - Andó, István TI - In Vivo Immunostaining of Hemocyte Compartments in Drosophila for Live Imaging JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 6 PG - 6 SN - 1932-6203 DO - 10.1371/journal.pone.0098191 UR - https://m2.mtmt.hu/api/publication/2708773 ID - 2708773 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Developmental Genetics Unit, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Cited By :5 Export Date: 31 January 2020 CODEN: POLNC Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Developmental Genetics Unit, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Cited By :8 Export Date: 20 April 2021 CODEN: POLNC AB - In recent years, Drosophila melanogaster has become an attractive model organism in which to study the structure and development of the cellular immune components. The emergence of immunological markers greatly accelerated the identification of the immune cells (hemocytes), while the creation of genetic reporter constructs allowed unique insight into the structural organization of hematopoietic tissues. However, investigation of the hemocyte compartments by the means of immunological markers requires dissection and fixation, which regularly disrupt the delicate structure and hamper the microanatomical characterization. Moreover, the investigation of transgenic reporters alone can be misleading as their expression often differs from the native expression pattern of their respective genes. We describe here a method that combines the reporter constructs and the immunological tools in live imaging, thereby allowing use of the array of available immunological markers while retaining the structural integrity of the hematopoietic compartments. The procedure allows the reversible immobilization of Drosophila larvae for high-resolution confocal imaging and the time-lapse video analysis of in vivo reporters. When combined with our antibody injection-based in situ immunostaining assay, the resulting double labeling of the hemocyte compartments can provide new information on the microanatomy and functional properties of the hematopoietic tissues in an intact state. Although this method was developed to study the immune system of Drosophila melanogaster, we anticipate that such a combination of genetic and immunological markers could become a versatile technique for in vivo studies in other biological systems too. LA - English DB - MTMT ER - TY - JOUR AU - Fors, L AU - Markus, R AU - Theopold, U AU - Hamback, PA TI - Differences in Cellular Immune Competence Explain Parasitoid Resistance for Two Coleopteran Species JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 9 PG - 8 SN - 1932-6203 DO - 10.1371/journal.pone.0108795 UR - https://m2.mtmt.hu/api/publication/24356904 ID - 24356904 N1 - Cited By :19 Export Date: 30 June 2022 CODEN: POLNC LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Csordás, Gábor AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Andó, István TI - The cell-mediated immunity of Drosophila melanogaster: Hemocyte lineages, immune compartments, microanatomy and regulation. JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 42 PY - 2014 IS - 1 SP - 47 EP - 56 PG - 10 SN - 0145-305X DO - 10.1016/j.dci.2013.06.005 UR - https://m2.mtmt.hu/api/publication/2372553 ID - 2372553 N1 - Cited By :124 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Andó, I.; Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary; email: ando.istvan@brc.mta.hu AB - In the animal kingdom, innate immunity is the first line of defense against invading pathogens. The dangers of microbial and parasitic attacks are countered by similar mechanisms, involving the prototypes of the cell-mediated immune responses, the phagocytosis and encapsulation. Work on Drosophila has played an important role in promoting an understanding of the basic mechanisms of phylogenetically conserved modules of innate immunity. The aim of this review is to survey the developments in the identification and functional definition of immune cell types and the immunological compartments of Drosophila melanogaster. We focus on the molecular and developmental aspects of the blood cell types and compartments, as well as the dynamics of blood cell development and the immune response. Further advances in the characterization of the innate immune mechanisms in Drosophila will provide basic clues to the understanding of the importance of the evolutionary conserved mechanisms of innate immune defenses in the animal kingdom. LA - English DB - MTMT ER - TY - JOUR AU - Myllymaki, H AU - Ramet, M TI - JAK/STAT Pathway in Drosophila Immunity JF - SCANDINAVIAN JOURNAL OF IMMUNOLOGY J2 - SCAND J IMMUNOL VL - 79 PY - 2014 IS - 6 SP - 377 EP - 385 PG - 9 SN - 0300-9475 DO - 10.1111/sji.12170 UR - https://m2.mtmt.hu/api/publication/24399545 ID - 24399545 LA - English DB - MTMT ER - TY - JOUR AU - Schmid, Martin R AU - Anderl, Ines AU - Vesala, Laura AU - Vanha-aho, Leena-Maija AU - Deng, Xiao-Juan AU - Ramet, Mika AU - Hultmark, Dan TI - Control of Drosophila Blood Cell Activation via Toll Signaling in the Fat Body JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 8 PG - 10 SN - 1932-6203 DO - 10.1371/journal.pone.0102568 UR - https://m2.mtmt.hu/api/publication/24874773 ID - 24874773 LA - English DB - MTMT ER - TY - JOUR AU - Small, C. AU - Ramroop, J. AU - Otazo, M. AU - Huang, L.H. AU - Saleque, S. AU - Govind, S. TI - An unexpected link between notch signaling and ROS in restricting the differentiation of hematopoietic progenitors in Drosophila JF - GENETICS J2 - GENETICS VL - 197 PY - 2014 IS - 2 SP - 471 EP - 483 PG - 13 SN - 0016-6731 DO - 10.1534/genetics.113.159210 UR - https://m2.mtmt.hu/api/publication/32913596 ID - 32913596 N1 - Cited By :37 Export Date: 30 June 2022 CODEN: GENTA AB - A fundamental question in hematopoietic development is how multipotent progenitors achieve precise identities, while the progenitors themselves maintain quiescence. In Drosophila melanogaster larvae, multipotent hematopoietic progenitors support the production of three lineages, exhibit quiescence in response to cues from a niche, and from their differentiated progeny. Infection by parasitic wasps alters the course of hematopoiesis. Here we address the role of Notch (N) signaling in lamellocyte differentiation in response to wasp infection. We show that Notch activity is moderately high and ubiquitous in all cells of the lymph gland lobes, with crystal cells exhibiting the highest levels. Wasp infection reduces Notch activity, which results in fewer crystal cells and more lamellocytes. Robust lamellocyte differentiation is induced even in N mutants. Using RNA interference knockdown of N, Serrate, and neuralized (neur), and twin clone analysis of a N null allele, we show that all three genes inhibit lamellocyte differentiation. However, unlike its cell-autonomous function in crystal cell development, Notch's inhibitory influence on lamellocyte differentiation is not cell autonomous. High levels of reactive oxygen species in the lymph gland lobes, but not in the niche, accompany NRNAi-induced lamellocyte differentiation and lobe dispersal. Our results define a novel dual role for Notch signaling in maintaining competence for basal hematopoiesis: while crystal cell development is encouraged, lamellocytic fate remains repressed. Repression of Notch signaling in fly hematopoiesis is important for host defense against natural parasitic wasp infections. These findings can serve as a model to understand how reactive oxygen species and Notch signals are integrated and interpreted in vivo. © 2014 by the Genetics Society of America. LA - English DB - MTMT ER - TY - JOUR AU - Fossett, N TI - Signal transduction pathways, intrinsic regulators, and the control of cell fate choice JF - BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS J2 - BBA-GEN SUBJECTS VL - 1830 PY - 2013 IS - 2 SP - 2375 EP - 2384 PG - 10 SN - 0304-4165 DO - 10.1016/j.bbagen.2012.06.005 UR - https://m2.mtmt.hu/api/publication/23238862 ID - 23238862 LA - English DB - MTMT ER - TY - JOUR AU - Gao, Hongjuan AU - Wu, Xiaorong AU - Fossett, Nancy TI - Drosophila E-Cadherin Functions in Hematopoietic Progenitors to Maintain Multipotency and Block Differentiation JF - PLOS ONE J2 - PLOS ONE VL - 8 PY - 2013 IS - 9 PG - 14 SN - 1932-6203 DO - 10.1371/journal.pone.0074684 UR - https://m2.mtmt.hu/api/publication/25022905 ID - 25022905 N1 - Megjegyzés-23469603 N1 Funding Details: NIH, National Institutes of Health Megjegyzés-23469622 N1 Funding Details: NIH, National Institutes of Health Megjegyzés-23469598 N1 Funding Details: NIH, National Institutes of Health LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Kurucz, Judit Éva AU - Zsámboki, János AU - Evans, CJ AU - Banerjee, U AU - Andó, István TI - Variation of NimC1 expression in Drosophila stocks and transgenic strains. JF - FLY J2 - FLY VL - 7 PY - 2013 IS - 4 SP - 263 EP - 266 PG - 4 SN - 1933-6934 DO - 10.4161/fly.25654 UR - https://m2.mtmt.hu/api/publication/2372552 ID - 2372552 AB - The NimC1 molecule has been described as a phagocytosis receptor, and is being used as a marker for professional phagocytes, the plasmatocytes, in Drosophila melanogaster. In studies including tumor-biology, developmental biology, and cell mediated immunity, monoclonal antibodies (P1a and P1b) to the NimC1 antigen are used. As we observed that these antibodies did not react with plasmatocytes of several strains and genetic combinations, a molecular analysis was performed on the structure of the nimC1 gene. We found 2 deletions and an insertion within the nimC1 gene, which may result in the production of a truncated NimC1 protein. The NimC1 positivity was regained by recombining the mutation with a wild-type allele or by using nimC1 mutant lines under heterozygous conditions. By means of these procedures or gaining access to the recombined stock, NimC1 can be used as a marker for phagocytic cells in the majority of the possible genetic backgrounds. LA - English DB - MTMT ER - TY - JOUR AU - King, JG AU - Hillyer, JF TI - Spatial and temporal in vivo analysis of circulating and sessile immune cells in mosquitoes: hemocyte mitosis following infection JF - BMC BIOLOGY J2 - BMC BIOL VL - 11 PY - 2013 SN - 1741-7007 DO - 10.1186/1741-7007-11-55 UR - https://m2.mtmt.hu/api/publication/23238861 ID - 23238861 LA - English DB - MTMT ER - TY - CHAP AU - McGarry, MP TI - The Evolutionary Origins and Presence of Eosinophils in Extant Species T2 - Eosinophils in Health and Disease PB - Elsevier Inc. SN - 9780123943859 PB - Elsevier Inc. PY - 2013 SP - 13 EP - 18 PG - 6 DO - 10.1016/B978-0-12-394385-9.00002-X UR - https://m2.mtmt.hu/api/publication/23469621 ID - 23469621 LA - English DB - MTMT ER - TY - JOUR AU - Havard, S AU - Doury, G AU - Ravallec, M AU - Brehélin, M AU - Prévost, G AU - Eslin, P TI - Structural and functional characterization of pseudopodocyte, a shaggy immune cell produced by two Drosophila species of the obscura group JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 36 PY - 2012 IS - 2 SP - 323 EP - 331 PG - 300 SN - 0145-305X DO - 10.1016/j.dci.2011.05.009 UR - https://m2.mtmt.hu/api/publication/21893824 ID - 21893824 N1 - Cited By :13 Export Date: 30 June 2022 CODEN: DCIMD LA - English DB - MTMT ER - TY - JOUR AU - Kacsoh, BZ AU - Schlenke, TA TI - High Hemocyte Load Is Associated with Increased Resistance against Parasitoids in Drosophila suzukii, a Relative of D. melanogaster JF - PLOS ONE J2 - PLOS ONE VL - 7 PY - 2012 IS - 4 SN - 1932-6203 DO - 10.1371/journal.pone.0034721 UR - https://m2.mtmt.hu/api/publication/22865289 ID - 22865289 LA - English DB - MTMT ER - TY - JOUR AU - Keebaugh, ES AU - Schlenke, TA TI - Adaptive Evolution of a Novel Drosophila Lectin Induced by Parasitic Wasp Attack JF - MOLECULAR BIOLOGY AND EVOLUTION J2 - MOL BIOL EVOL VL - 29 PY - 2012 IS - 2 SP - 565 EP - 577 PG - 13 SN - 0737-4038 DO - 10.1093/molbev/msr191 UR - https://m2.mtmt.hu/api/publication/22865270 ID - 22865270 LA - English DB - MTMT ER - TY - JOUR AU - Sampson, CJ AU - Valanne, S AU - Fauvarque, MO AU - Hultmark, D AU - Ramet, M AU - Williams, MJ TI - The RhoGEF Zizimin-related acts in the Drosophila cellular immune response via the Rho GTPases Rac2 and Cdc42 JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 38 PY - 2012 IS - 1 SP - 160 EP - 168 PG - 9 SN - 0145-305X DO - 10.1016/j.dci.2012.05.004 UR - https://m2.mtmt.hu/api/publication/22865283 ID - 22865283 N1 - Funding Agency and Grant Number: Swedish Research Council; Swedish Cancer Society; College of Life Sciences and Medicine, University of Aberdeen, Aberdeen, UK Funding text: The authors would like to thank Dr. L Van Aelst for the human Dock7 antibody. The work presented was funded in part by Dan Hultmark's grants from the Swedish Research Council and the Swedish Cancer Society. It was also supported by funds provided by the College of Life Sciences and Medicine, University of Aberdeen, Aberdeen, UK. LA - English DB - MTMT ER - TY - JOUR AU - Zaidman-Remy, A AU - Regan, JC AU - Brandao, AS AU - Jacinto, A TI - The Drosophila larva as a tool to study gut-associated macrophages: PI3K regulates a discrete hemocyte population at the proventriculus JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 36 PY - 2012 IS - 4 SP - 638 EP - 647 PG - 10 SN - 0145-305X DO - 10.1016/j.dci.2011.10.013 UR - https://m2.mtmt.hu/api/publication/22865258 ID - 22865258 N1 - Funding Agency and Grant Number: ARC; FCT [SFRH/BPD/44613/2008]; EMBO [ALTF 178-2009]; [PTDC/BIA-BCM/65872/2006]; [ERC-RESEAL/208631]; Fundação para a Ciência e a Tecnologia [SFRH/BPD/44613/2008] Funding Source: FCT Funding text: We are grateful to L. Teixeira, E. Sucena, A. Leith, J. Veenstra, L. Carvalho, I. Campos, K. Hanson, P. Liehl, E. Liehl, N. Matova and S. Prag for discussion, J. Rino and A. Temudo for assistance with confocal microscopes, C. Carret for suggestions on statistical analyses, I. Matos, A. Dias, M. Carapuco and A. Roberto for technical support, and T. Tokusumi, F. Missirlis, S. Sinenko S. Prag and P. Worth for providing fly lines. This work was supported by PTDC/BIA-BCM/65872/2006 and ERC-RESEAL/208631 Grants (A.J.), ARC and FCT SFRH/BPD/44613/2008 (A.Z.R.) and EMBO ALTF 178-2009 (J.R.). 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