TY - JOUR AU - Kinoshita, Yuriko AU - Shiratsuchi, Naoka AU - Araki, Mayo AU - Inoue, Yoshihiro H. TI - Anti-Tumor Effect of Turandot Proteins Induced via the JAK/STAT Pathway in the mxc Hematopoietic Tumor Mutant in Drosophila JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 16 PG - 21 SN - 2073-4409 DO - 10.3390/cells12162047 UR - https://m2.mtmt.hu/api/publication/34261481 ID - 34261481 AB - Several antimicrobial peptides suppress the growth of lymph gland (LG) tumors in Drosophila multi sex comb (mxc) mutant larvae. The activity of another family of polypeptides, called Turandots, is also induced via the JAK/STAT pathway after bacterial infection; however, their influence on Drosophila tumors remains unclear. The JAK/STAT pathway was activated in LG tumors, fat body, and circulating hemocytes of mutant larvae. The mRNA levels of Turandot (Tot) genes increased markedly in the mutant fat body and declined upon silencing Stat92E in the fat body, indicating the involvement of the JAK/STAT pathway. Furthermore, significantly enhanced tumor growth upon a fat-body-specific silencing of the mRNAs demonstrated the antitumor effects of these proteins. The proteins were found to be incorporated into small vesicles in mutant circulating hemocytes (as previously reported for several antimicrobial peptides) but not normal cells. In addition, more hemocytes containing these proteins were found to be associated with tumors. The mutant LGs contained activated effector caspases, and a fat-body-specific silencing of Tots inhibited apoptosis and increased the number of mitotic cells in the LG, thereby suggesting that the proteins inhibited tumor cell proliferation. Thus, Tot proteins possibly exhibit antitumor effects via the induction of apoptosis and inhibition of cell proliferation. LA - English DB - MTMT ER - TY - JOUR AU - Kúthy-Sutus, Enikő AU - Kharrat, Bayan AU - Gábor, Erika AU - Csordás, Gábor AU - Sinka, Rita AU - Honti, Viktor TI - A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 1 PG - 15 SN - 2073-4409 DO - 10.3390/cells12010024 UR - https://m2.mtmt.hu/api/publication/33555087 ID - 33555087 N1 - Export Date: 24 January 2023 AB - The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo. LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Magyar, Lilla Brigitta AU - Kovács, Attila Lajos AU - Lerner, Zita AU - Juhász, Gábor AU - Lukacsovich, David AU - Winterer, Jochen AU - Lukacsovich, Tamás AU - Hegedűs, Zoltán AU - Kurucz, Judit Éva AU - Hultmark, Dan AU - Földy, Csaba AU - Andó, István TI - Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 14 PY - 2022 IS - 4 SP - 335 EP - 354 PG - 20 SN - 1662-811X DO - 10.1159/000520110 UR - https://m2.mtmt.hu/api/publication/32524824 ID - 32524824 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Feng, M. AU - Swevers, L. AU - Sun, J. TI - Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 13 PY - 2022 SN - 1664-3224 DO - 10.3389/fimmu.2022.852702 UR - https://m2.mtmt.hu/api/publication/32876826 ID - 32876826 N1 - Export Date: 14 June 2022 AB - Within the hemolymph, insect hemocytes constitute a heterogeneous population of macrophage-like cells that play important roles in innate immunity, homeostasis and development. Classification of hemocytes in different subtypes by size, morphology and biochemical or immunological markers has been difficult and only in Drosophila extensive genetic analysis allowed the construction of a coherent picture of hemocyte differentiation from pro-hemocytes to granulocytes, crystal cells and plasmatocytes. However, the advent of high-throughput single cell technologies, such as single cell RNA sequencing (scRNA-seq), is bound to have a high impact on the study of hemocytes subtypes and their phenotypes in other insects for which a sophisticated genetic toolbox is not available. Instead of averaging gene expression across all cells as occurs in bulk-RNA-seq, scRNA-seq allows high-throughput and specific visualization of the differentiation status of individual cells. With scRNA-seq, interesting cell types can be identified in heterogeneous populations and direct analysis of rare cell types is possible. Next to its ability to profile the transcriptomes of individual cells in tissue samples, scRNA-seq can be used to propose marker genes that are characteristic of different hemocyte subtypes and predict their functions. In this perspective, the identities of the different marker genes that were identified by scRNA-seq analysis to define 13 distinct cell clusters of hemocytes in larvae of the silkworm, Bombyx mori, are discussed in detail. The analysis confirms the broad division of hemocytes in granulocytes, plasmatocytes, oenocytoids and perhaps spherulocytes but also reveals considerable complexity at the molecular level and highly specialized functions. In addition, predicted hemocyte marker genes in Bombyx generally show only limited convergence with the genes that are considered characteristic for hemocyte subtypes in Drosophila. Copyright © 2022 Feng, Swevers and Sun. LA - English DB - MTMT ER - TY - JOUR AU - Kharrat, Bayan AU - Csordás, Gábor AU - Honti, Viktor TI - Peeling Back the Layers of Lymph Gland Structure and Regulation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 14 PG - 16 SN - 1661-6596 DO - 10.3390/ijms23147767 UR - https://m2.mtmt.hu/api/publication/33050458 ID - 33050458 N1 - Cited By :2 Export Date: 24 January 2023 AB - During the past 60 years, the fruit fly, Drosophila melanogaster, has proven to be an excellent model to study the regulation of hematopoiesis. This is not only due to the evolutionarily conserved signalling pathways and transcription factors contributing to blood cell fate, but also to convergent evolution that led to functional similarities in distinct species. An example of convergence is the compartmentalization of blood cells, which ensures the quiescence of hematopoietic stem cells and allows for the rapid reaction of the immune system upon challenges. The lymph gland, a widely studied hematopoietic organ of the Drosophila larva, represents a microenvironment with similar features and functions to classical hematopoietic stem cell niches of vertebrates. Lymph gland studies were effectively supported by the unparalleled toolkit developed in Drosophila, which enabled the high-resolution investigation of the cellular composition and regulatory interaction networks of the lymph gland. In this review, we summarize how our understanding of lymph gland structure and hematopoietic cell-to-cell communication evolved during the past decades and compare their analogous features to those of the vertebrate hematopoietic stem cell niche. LA - English DB - MTMT ER - TY - JOUR AU - Balog, József Ágoston AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Kari, Beáta AU - Puskás, László AU - Andó, István AU - Szebeni, Gábor TI - Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry JF - GENOMICS PROTEOMICS & BIOINFORMATICS J2 - GENOM PROTEOM BIOINF VL - 19 PY - 2021 IS - 2 SP - 243 EP - 252 PG - 10 SN - 1672-0229 DO - 10.1016/j.gpb.2020.06.022 UR - https://m2.mtmt.hu/api/publication/31940120 ID - 31940120 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030, GINOP-2.3.2-15-2016-00035, NKFI NN118207, NKFI K120142, NKFI 120140, OTKA K-131484]; New National Excellence Program of the Ministry for Innovation and Technology, Hungary [UNKP-19-4-SZTE-36]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [BO/00139/17/8] Funding text: This work was supported by the grants from the National Research, Development and Innovation Office, Hungary (Grant Nos. GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030 to LGP, GINOP-2.3.2-15-2016-00035 to E ' K, NKFI NN118207 and NKFI K120142 to IA, NKFI 120140 to E ' K, and OTKA K-131484 to VH). Ga ' bor J. Szebeni was supported by the New National Excellence Program of the Ministry for Innovation and Technology, Hungary (Grant No. UNKP-19-4-SZTE-36) and by the Ja ' nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (Grant No. BO/00139/17/8). We are grateful to Mrs. Olga Kovalcsik for the technical help. LA - English DB - MTMT ER - TY - JOUR AU - Cattenoz, P.B. AU - Monticelli, S. AU - Pavlidaki, A. AU - Giangrande, A. TI - Toward a Consensus in the Repertoire of Hemocytes Identified in Drosophila JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 9 PY - 2021 SN - 2296-634X DO - 10.3389/fcell.2021.643712 UR - https://m2.mtmt.hu/api/publication/32006062 ID - 32006062 N1 - Export Date: 10 May 2021 Correspondence Address: Cattenoz, P.B.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Centre National de la Recherche Scientifique, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Centre National de la Recherche Scientifique, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Institut National de la Santé et de la Recherche MédicaleFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut National de la Santé et de la Recherche MédicaleFrance; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Université de StrasbourgFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Université de StrasbourgFrance; email: angela@igbmc.fr AB - The catalog of the Drosophila immune cells was until recently limited to three major cell types, based on morphology, function and few molecular markers. Three recent single cell studies highlight the presence of several subgroups, revealing a large diversity in the molecular signature of the larval immune cells. Since these studies rely on somewhat different experimental and analytical approaches, we here compare the datasets and identify eight common, robust subgroups associated to distinct functions such as proliferation, immune response, phagocytosis or secretion. Similar comparative analyses with datasets from different stages and tissues disclose the presence of larval immune cells resembling embryonic hemocyte progenitors and the expression of specific properties in larval immune cells associated with peripheral tissues. © Copyright © 2021 Cattenoz, Monticelli, Pavlidaki and Giangrande. LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Gábor, Erika AU - Honti, Viktor TI - There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 469 PY - 2021 SP - 135 EP - 143 PG - 9 SN - 0012-1606 DO - 10.1016/j.ydbio.2020.10.006 UR - https://m2.mtmt.hu/api/publication/31743832 ID - 31743832 N1 - Cited By :5 Export Date: 14 June 2022 CODEN: DEBIA LA - English DB - MTMT ER - TY - JOUR AU - Morin-Poulard, Ismael AU - Tian, Yushun AU - Vanzo, Nathalie AU - Crozatier, Michele TI - Drosophila as a Model to Study Cellular Communication Between the Hematopoietic Niche and Blood Progenitors Under Homeostatic Conditions and in Response to an Immune Stress JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 PG - 11 SN - 1664-3224 DO - 10.3389/fimmu.2021.719349 UR - https://m2.mtmt.hu/api/publication/32361805 ID - 32361805 N1 - Export Date: 19 January 2022 Correspondence Address: Crozatier, M.; MCD, France AB - In adult mammals, blood cells are formed from hematopoietic stem progenitor cells, which are controlled by a complex cellular microenvironment called "niche". Drosophila melanogaster is a powerful model organism to decipher the mechanisms controlling hematopoiesis, due both to its limited number of blood cell lineages and to the conservation of genes and signaling pathways throughout bilaterian evolution. Insect blood cells or hemocytes are similar to the mammalian myeloid lineage that ensures innate immunity functions. Like in vertebrates, two waves of hematopoiesis occur in Drosophila. The first wave takes place during embryogenesis. The second wave occurs at larval stages, where two distinct hematopoietic sites are identified: subcuticular hematopoietic pockets and a specialized hematopoietic organ called the lymph gland. In both sites, hematopoiesis is regulated by distinct niches. In hematopoietic pockets, sensory neurons of the peripheral nervous system provide a microenvironment that promotes embryonic hemocyte expansion and differentiation. In the lymph gland blood cells are produced from hematopoietic progenitors. A small cluster of cells called Posterior Signaling Centre (PSC) and the vascular system, along which the lymph gland develops, act collectively as a niche, under homeostatic conditions, to control the balance between maintenance and differentiation of lymph gland progenitors. In response to an immune stress such as wasp parasitism, lymph gland hematopoiesis is drastically modified and shifts towards emergency hematopoiesis, leading to increased progenitor proliferation and their differentiation into lamellocyte, a specific blood cell type which will neutralize the parasite. The PSC is essential to control this emergency response. In this review, we summarize Drosophila cellular and molecular mechanisms involved in the communication between the niche and hematopoietic progenitors, both under homeostatic and stress conditions. Finally, we discuss similarities between mechanisms by which niches regulate hematopoietic stem/progenitor cells in Drosophila and mammals. LA - English DB - MTMT ER - TY - JOUR AU - Spratford, C.M. AU - Goins, L.M. AU - Chi, F. AU - Girard, J.R. AU - Macias, S.N. AU - Ho, V.W. AU - Banerjee, U. TI - Intermediate progenitor cells provide a transition between hematopoietic progenitors and their differentiated descendants JF - DEVELOPMENT J2 - DEVELOPMENT VL - 148 PY - 2021 IS - 24 SN - 0950-1991 DO - 10.1242/dev.200216 UR - https://m2.mtmt.hu/api/publication/32601905 ID - 32601905 N1 - Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, United States Molecular Biology Institute, University of California, Los Angeles, United States Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, United States Department of Biological Chemistry, University of California, Los Angeles, United States Export Date: 19 January 2022 CODEN: DEVPE Correspondence Address: Banerjee, U.; Department of Molecular, United States; email: banerjee@mbi.ucla.edu AB - Genetic and genomic analysis in Drosophila suggests that hematopoietic progenitors likely transition into terminal fates via intermediate progenitors (IPs) with some characteristics of either, but perhaps maintaining IP-specific markers. In the past, IPs have not been directly visualized and investigated owing to lack of appropriate genetic tools. Here, we report a Split GAL4 construct, CHIZ-GAL4, that identifies IPs as cells physically juxtaposed between true progenitors and differentiating hemocytes. IPs are a distinct cell type with a unique cell-cycle profile and they remain multipotent for all blood cell fates. In addition, through their dynamic control of the Notch ligand Serrate, IPs specify the fate of direct neighbors. The Ras pathway controls the number of IP cells and promotes their transition into differentiating cells. This study suggests that it would be useful to characterize such intermediate populations of cells in mammalian hematopoietic systems. © 2021. Published by The Company of Biologists Ltd LA - English DB - MTMT ER - TY - JOUR AU - Wan, Bin AU - Belghazi, Maya AU - Lemauf, Severine AU - Poirie, Marylene AU - Gatti, Jean-Luc TI - Proteomics of purified lamellocytes from Drosophila melanogaster HopTum-l identifies new membrane proteins and networks involved in their functions JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 134 PY - 2021 PG - 21 SN - 0965-1748 DO - 10.1016/j.ibmb.2021.103584 UR - https://m2.mtmt.hu/api/publication/32358968 ID - 32358968 N1 - Université Côte d'Azur, INRAE, CNRS, Institute Sophia-Agrobiotech, Sophia Antipolis, France Institute of NeuroPhysiopathology (INP), UMR7051, CNRS, Aix-Marseille Université, Marseille, 13015, France Cited By :1 Export Date: 19 January 2022 CODEN: IBMBE Correspondence Address: Gatti, J.-L.; Sophia Agrobiotech Institute (ISA), Sophia Antipolis, France; email: jean-luc.gatti@inrae.fr AB - In healthy Drosophila melanogaster larvae, plasmatocytes and crystal cells account for 95% and 5% of the hemocytes, respectively. A third type of hemocytes, lamellocytes, are rare, but their number increases after oviposition by parasitoid wasps. The lamellocytes form successive layers around the parasitoid egg, leading to its encapsulation and melanization, and finally the death of this intruder. However, the total number of lamellocytes per larva remains quite low even after parasitoid infestation, making direct biochemical studies difficult. Here, we used the HopTum-l mutant strain that constitutively produces large numbers of lamellocytes to set up a purification method and analyzed their major proteins by 2D gel electrophoresis and their plasma membrane surface proteins by 1D SDS-PAGE after affinity purification. Mass spectrometry identified 430 proteins from 2D spots and 344 affinity-purified proteins from 1D bands, for a total of 639 unique proteins. Known lamellocyte markers such as PPO3 and the myospheroid integrin were among the components identified with specific chaperone proteins. Affinity purification detected other integrins, as well as a wide range of integrin-associated proteins involved in the formation and function of cell-cell junctions. Overall, the newly identified proteins indicate that these cells are highly adapted to the encapsulation process (recognition, motility, adhesion, signaling), but may also have several other physiological functions (such as secretion and internalization of vesicles) under different signaling pathways. These results provide the basis for further in vivo and in vitro studies of lamellocytes, including the development of new markers to identify coexisting populations and their respective origins and functions in Drosophila immunity. LA - English DB - MTMT ER - TY - JOUR AU - Cattenoz, Pierre B. AU - Sakr, Rosy AU - Pavlidaki, Alexia AU - Delaporte, Claude AU - Riba, Andrea AU - Molina, Nacho AU - Hariharan, Nivedita AU - Mukherjee, Tina AU - Giangrande, Angela TI - Temporal specificity and heterogeneity ofDrosophilaimmune cells JF - EMBO JOURNAL J2 - EMBO J VL - 39 PY - 2020 IS - 12 PG - 25 SN - 0261-4189 DO - 10.15252/embj.2020104486 UR - https://m2.mtmt.hu/api/publication/31460653 ID - 31460653 N1 - Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France Centre National de la Recherche Scientifique, UMR7104, Illkirch, France Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France Université de Strasbourg, Illkirch, France Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, India The University of Trans-disciplinary Health Sciences and Technology, Bangalore, India Cited By :31 Export Date: 19 January 2022 CODEN: EMJOD Correspondence Address: Cattenoz, P.B.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Centre National de la Recherche Scientifique, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Centre National de la Recherche Scientifique, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Institut National de la Santé et de la Recherche Médicale, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut National de la Santé et de la Recherche Médicale, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Université de StrasbourgFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Université de StrasbourgFrance; email: angela@igbmc.fr AB - Immune cells provide defense against non-self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity ofDrosophilaimmune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single-cell RNA sequencing, we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct processes, e.g., proliferation, phagocytosis, metabolic homeostasis, and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation, which triggers the differentiation of a novel hemocyte type, the lamellocyte. This first molecular atlas of hemocytes provides insights and paves the way to study the biology of theDrosophilaimmune cells in physiological and pathological conditions. LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Lerner, Zita AU - Magyar, Lilla Brigitta AU - Soós, Bálint AU - Tóth, Renáta AU - Kristó, Ildikó AU - Vilmos, Péter AU - Juhász, Gábor AU - Kovács, Attila Lajos AU - Hegedűs, Zoltán AU - Sensen, Christoph W. AU - Kurucz, Judit Éva AU - Andó, István TI - Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid Zaprionus indianus JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 12 PY - 2020 IS - 3 SP - 257 EP - 272 PG - 16 SN - 1662-811X DO - 10.1159/000502646 UR - https://m2.mtmt.hu/api/publication/30819399 ID - 30819399 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Fu, Yulong AU - Huang, Xiaohu AU - Zhang, Peng AU - van de Leemput, Joyce AU - Han, Zhe TI - Single-cell RNA sequencing identifies novel cell types in Drosophila blood JF - JOURNAL OF GENETICS AND GENOMICS J2 - J GENET GENOMICS VL - 47 PY - 2020 IS - 4 SP - 175 EP - 186 PG - 12 SN - 1673-8527 DO - 10.1016/j.jgg.2020.02.004 UR - https://m2.mtmt.hu/api/publication/31469242 ID - 31469242 N1 - Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, United States Divisions of Immunotherapy, University of Maryland School of Medicine, Baltimore, MD 21201, United States Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, United States Cited By :21 Export Date: 19 January 2022 Correspondence Address: Han, Z.; Center for Precision Disease Modeling, 670 West Baltimore Street, 4052 HSF3, United States; email: zhan@som.umaryland.edu AB - Drosophila has been extensively used to model the human blood-immune system, as both systems share many developmental and immune response mechanisms. However, while many human blood cell types have been identified, only three were found in flies: plasmatocytes, crystal cells and lamellocytes. To better understand the complexity of fly blood system, we used single-cell RNA sequencing technology to generate comprehensive gene expression profiles for Drosophila circulating blood cells. In addition to the known cell types, we identified two new Drosophila blood cell types: thanacytes and primocytes. Thanacytes, which express many stimulus response genes, are involved in distinct responses to different types of bacteria. Primocytes, which express cell fate commitment and signaling genes, appear to be involved in keeping stem cells in the circulating blood. Furthermore, our data revealed four novel plasmatocyte subtypes (Ppn(+), CAH7(+), Lsp(+) and reservoir plasmatocytes), each with unique molecular identities and distinct predicted functions. We also identified cross-species markers from Drosophila hemocytes to human blood cells. Our analysis unveiled a more complex Drosophila blood system and broadened the scope of using Drosophila to model human blood system in development and disease. Copyright (C) 2020, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Wan, Bin AU - Yang, Lei AU - Zhang, Jiao AU - Qiu, Liming AU - Fang, Qi AU - Yao, Hongwei AU - Poirie, Marylene AU - Gatti, Jean-Luc AU - Ye, Gongyin TI - The Venom of the Ectoparasitoid WaspPachycrepoideus vindemiae(Hymenoptera: Pteromalidae) Induces Apoptosis ofDrosophila melanogasterHemocytes JF - INSECTS J2 - INSECTS VL - 11 PY - 2020 IS - 6 PG - 14 SN - 2075-4450 DO - 10.3390/insects11060363 UR - https://m2.mtmt.hu/api/publication/31470141 ID - 31470141 AB - The pupal ectoparasitoidPachycrepoideus vindemiaeinjects venom into its fly hosts prior to oviposition. We have shown that this venom causes immune suppression inDrosophila melanogasterpupa but the mechanism involved remained unclear. Here, we show using transgenicD. melanogasterwith fluorescent hemocytes that the in vivo number of plasmatocytes and lamellocytes decreases after envenomation while it has a limited effect on crystal cells. After in vitro incubation with venom, the cytoskeleton of plasmatocytes underwent rearrangement with actin aggregation around the internal vacuoles, which increased with incubation time and venom concentration. The venom also decreased the lamellocytes adhesion capacity and induced nucleus fragmentation. Electron microscopy observation revealed that the shape of the nucleus and mitochondria became irregular after in vivo incubation with venom and confirmed the increased vacuolization with the formation of autophagosomes-like structures. Almost all venom-treated hemocytes became positive for TUNEL assays, indicating massive induced apoptosis. In support, the caspase inhibitor Z-VAD-FMK attenuated the venom-induced morphological changes suggesting an involvement of caspases. Our data indicate thatP. vindemiaevenom inhibitsD. melanogasterhost immunity by inducing strong apoptosis in hemocytes. These assays will help identify the individual venom component(s) responsible and the precise mechanism(s)/pathway(s) involved. LA - English DB - MTMT ER - TY - JOUR AU - Banerjee, Utpal AU - Girard, Juliet R. AU - Goins, Lauren M. AU - Spratford, Carrie M. TI - Drosophila as a Genetic Model for Hematopoiesis JF - GENETICS J2 - GENETICS VL - 211 PY - 2019 IS - 2 SP - 367 EP - 417 PG - 51 SN - 0016-6731 DO - 10.1534/genetics.118.300223 UR - https://m2.mtmt.hu/api/publication/30510237 ID - 30510237 N1 - Export Date: 13 May 2019 CODEN: GENTA Correspondence Address: Banerjee, U.; University of California, 610 Charles E Young Drive East, United States; email: banerjee@mbi.ucla.edu AB - In this FlyBook chapter, we present a survey of the current literature on the development of the hematopoietic system in Drosophila. The Drosophila blood system consists entirely of cells that function in innate immunity, tissue integrity, wound healing, and various forms of stress response, and are therefore functionally similar to myeloid cells in mammals. The primary cell types are specialized for phagocytic, melanization, and encapsulation functions. As in mammalian systems, multiple sites of hematopoiesis are evident in Drosophila and the mechanisms involved in this process employ many of the same molecular strategies that exemplify blood development in humans. Drosophila blood progenitors respond to internal and external stress by coopting developmental pathways that involve both local and systemic signals. An important goal of these Drosophila studies is to develop the tools and mechanisms critical to further our understanding of human hematopoiesis during homeostasis and dysfunction. LA - English DB - MTMT ER - TY - JOUR AU - Varga, Gergely István AU - Csordás, Gábor AU - Cinege, Gyöngyi Ilona AU - Jankovics, Ferenc AU - Sinka, Rita AU - Kurucz, Judit Éva AU - Andó, István AU - Honti, Viktor TI - Headcase is a Repressor of Lamellocyte Fate in Drosophila melanogaster JF - GENES J2 - GENES-BASEL VL - 10 PY - 2019 IS - 3 PG - 17 SN - 2073-4425 DO - 10.3390/genes10030173 UR - https://m2.mtmt.hu/api/publication/30585796 ID - 30585796 N1 - Laboratory of Immunology, Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Laboratory of Drosophila Germ Cell Differentiation, Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Department of Genetics, Faculty of Science and Informatics, University of Szeged, Szeged, 6726, Hungary Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, 50931, Germany Cited By :3 Export Date: 19 January 2022 Correspondence Address: Andó, I.; Laboratory of Immunology, Hungary; email: ando.istvan@brc.mta.hu AB - Due to the evolutionary conservation of the regulation of hematopoiesis, Drosophila provides an excellent model organism to study blood cell differentiation and hematopoietic stem cell (HSC) maintenance. The larvae of Drosophila melanogaster respond to immune induction with the production of special effector blood cells, the lamellocytes, which encapsulate and subsequently kill the invader. Lamellocytes differentiate as a result of a concerted action of all three hematopoietic compartments of the larva: the lymph gland, the circulating hemocytes, and the sessile tissue. Within the lymph gland, the communication of the functional zones, the maintenance of HSC fate, and the differentiation of effector blood cells are regulated by a complex network of signaling pathways. Applying gene conversion, mutational analysis, and a candidate based genetic interaction screen, we investigated the role of Headcase (Hdc), the homolog of the tumor suppressor HECA in the hematopoiesis of Drosophila. We found that naive loss-of-function hdc mutant larvae produce lamellocytes, showing that Hdc has a repressive role in effector blood cell differentiation. We demonstrate that hdc genetically interacts with the Hedgehog and the Decapentaplegic pathways in the hematopoietic niche of the lymph gland. By adding further details to the model of blood cell fate regulation in the lymph gland of the larva, our findings contribute to the better understanding of HSC maintenance. LA - English DB - MTMT ER - TY - JOUR AU - Wan, Bin AU - Goguet, Emilie AU - Ravallec, Marc AU - Pierre, Olivier AU - Lemauf, Severine AU - Volkoff, Anne-Nathalie AU - Gatti, Jean-Luc AU - Poirie, Marylone TI - Venom Atypical Extracellular Vesicles as Interspecies Vehicles of Virulence Factors Involved in Host Specificity: The Case of a Drosophila Parasitoid Wasp JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 10 PY - 2019 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2019.01688 UR - https://m2.mtmt.hu/api/publication/30907274 ID - 30907274 N1 - Université Côte d'Azur, CNRS, ISA, Sophia Antipolis, France Univ. Montpellier, UMR 1333 'Microorganism and Insect Diversity, Genomes and Interactions' (DGIMI), Montpellier, France State Key Laboratory of Rice Biology and Ministry of Agricultural and Rural Affairs, Institute of Insect Sciences, Zhejiang University, Hangzhou, China Henry M. Jackson Foundation at the Uniformed Services University of the Health Sciences, Department of Microbiology and Immunology, Bethesda, MD, United States Cited By :19 Export Date: 19 January 2022 Correspondence Address: Poirié, M.; Université Côte d'Azur, France; email: marylene.poirie@univ-cotedazur.fr AB - Endoparasitoid wasps, which lay eggs inside the bodies of other insects, use various strategies to protect their offspring from the host immune response. The hymenopteran species of the genus Leptopilina, parasites of Drosophila, rely on the injection of a venom which contains proteins and peculiar vesicles (hereafter venosomes). We show here that the injection of purified L. boulardi venosomes is sufficient to impair the function of the Drosophila melanogaster lamellocytes, a hemocyte type specialized in the defense against wasp eggs, and thus the parasitic success of the wasp. These venosomes seem to have a unique extracellular biogenesis in the wasp venom apparatus where they acquire specific secreted proteins/virulence factors and act as a transport system to deliver these compounds into host lamellocytes. The level of venosomes entry into lamellocytes of different Drosophila species was correlated with the rate of parasitism success of the wasp, suggesting that this venosome-cell interaction may represent a new evolutionary level of host-parasitoid specificity. LA - English DB - MTMT ER - TY - JOUR AU - Anderl, I AU - Vesala, L AU - Ihalainen, TO AU - Vanha-Aho, LM AU - Andó, István AU - Ramet, M AU - Hultmark, D TI - Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection. JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 12 PY - 2016 IS - 7 SP - e1005746 SN - 1553-7366 DO - 10.1371/journal.ppat.1005746 UR - https://m2.mtmt.hu/api/publication/3096913 ID - 3096913 N1 - WoS:hiba:000383366400030 2019-03-03 19:41 első oldal nem egyezik AB - Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail. LA - English DB - MTMT ER - TY - JOUR AU - Guillou, Aurelien AU - Troha, Katia AU - Wang, Hui AU - Franc, Nathalie C AU - Buchon, Nicolas TI - The Drosophila CD36 Homologue croquemort Is Required to Maintain Immune and Gut Homeostasis during Development and Aging JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 12 PY - 2016 IS - 10 PG - 27 SN - 1553-7366 DO - 10.1371/journal.ppat.1005961 UR - https://m2.mtmt.hu/api/publication/26377211 ID - 26377211 N1 - Department of Entomology, Cornell University, Ithaca, NY, United States Department of Cell & Molecular Biology, The Scripps Research Institute, La Jolla, CA, United States Cited By :33 Export Date: 19 January 2022 Correspondence Address: Buchon, N.; Department of Entomology, United States; email: nicolas.buchon@cornell.edu LA - English DB - MTMT ER - TY - JOUR AU - Kari, Beáta AU - Csordás, Gábor AU - Honti, Viktor AU - Cinege, Gyöngyi Ilona AU - Williams, MJ AU - Andó, István AU - Kurucz, Judit Éva TI - The raspberry Gene Is Involved in the Regulation of the Cellular Immune Response in Drosophila melanogaster JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 3 PG - 13 SN - 1932-6203 DO - 10.1371/journal.pone.0150910 UR - https://m2.mtmt.hu/api/publication/3045263 ID - 3045263 N1 - Biological Research Centre of Hungarian Academy of Sciences, Immunology Unit, Institute of Genetics, Szeged, Hungary Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden Cited By :6 Export Date: 14 February 2021 CODEN: POLNC AB - Drosophila is an extremely useful model organism for understanding how innate immune mechanisms defend against microbes and parasitoids. Large foreign objects trigger a potent cellular immune response in Drosophila larva. In the case of endoparasitoid wasp eggs, this response includes hemocyte proliferation, lamellocyte differentiation and eventual encapsulation of the egg. The encapsulation reaction involves the attachment and spreading of hemocytes around the egg, which requires cytoskeletal rearrangements, changes in adhesion properties and cell shape, as well as melanization of the capsule. Guanine nucleotide metabolism has an essential role in the regulation of pathways necessary for this encapsulation response. Here, we show that the Drosophila inosine 5'-monophosphate dehydrogenase (IMPDH), encoded by raspberry (ras), is centrally important for a proper cellular immune response against eggs from the parasitoid wasp Leptopilina boulardi. Notably, hemocyte attachment to the egg and subsequent melanization of the capsule are deficient in hypomorphic ras mutant larvae, which results in a compromised cellular immune response and increased survival of the parasitoid. LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Andó, István TI - Innate immunity JF - ACTA BIOLOGICA SZEGEDIENSIS J2 - ACTA BIOL SZEGED VL - 59 PY - 2015 IS - Suppl. 1 SP - 1 EP - 15 PG - 15 SN - 1588-385X UR - https://m2.mtmt.hu/api/publication/2993019 ID - 2993019 N1 - Export Date: 19 January 2022 CODEN: ABSCC Correspondence Address: Andó, I.; Immunology Unit, Hungary; email: ando@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Reitman, Zachary J AU - Sinenko, Sergey A AU - Spana, Eric P AU - Yan, Hai TI - Genetic dissection of leukemia-associated IDH1 and IDH2 mutants and D-2-hydroxyglutarate in Drosophila JF - BLOOD J2 - BLOOD VL - 125 PY - 2015 IS - 2 SP - 336 EP - 345 PG - 10 SN - 0006-4971 DO - 10.1182/blood-2014-05-577940 UR - https://m2.mtmt.hu/api/publication/24797539 ID - 24797539 N1 - Export Date: 20 April 2021 CODEN: BLOOA Export Date: 22 April 2021 CODEN: BLOOA LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Varga, Gergely István AU - Honti, Viktor AU - Jankovics, Ferenc AU - Kurucz, Judit Éva AU - Andó, István TI - In Vivo Immunostaining of Hemocyte Compartments in Drosophila for Live Imaging JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 6 PG - 6 SN - 1932-6203 DO - 10.1371/journal.pone.0098191 UR - https://m2.mtmt.hu/api/publication/2708773 ID - 2708773 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Developmental Genetics Unit, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Cited By :5 Export Date: 31 January 2020 CODEN: POLNC Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Developmental Genetics Unit, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Cited By :8 Export Date: 20 April 2021 CODEN: POLNC AB - In recent years, Drosophila melanogaster has become an attractive model organism in which to study the structure and development of the cellular immune components. The emergence of immunological markers greatly accelerated the identification of the immune cells (hemocytes), while the creation of genetic reporter constructs allowed unique insight into the structural organization of hematopoietic tissues. However, investigation of the hemocyte compartments by the means of immunological markers requires dissection and fixation, which regularly disrupt the delicate structure and hamper the microanatomical characterization. Moreover, the investigation of transgenic reporters alone can be misleading as their expression often differs from the native expression pattern of their respective genes. We describe here a method that combines the reporter constructs and the immunological tools in live imaging, thereby allowing use of the array of available immunological markers while retaining the structural integrity of the hematopoietic compartments. The procedure allows the reversible immobilization of Drosophila larvae for high-resolution confocal imaging and the time-lapse video analysis of in vivo reporters. When combined with our antibody injection-based in situ immunostaining assay, the resulting double labeling of the hemocyte compartments can provide new information on the microanatomy and functional properties of the hematopoietic tissues in an intact state. Although this method was developed to study the immune system of Drosophila melanogaster, we anticipate that such a combination of genetic and immunological markers could become a versatile technique for in vivo studies in other biological systems too. LA - English DB - MTMT ER - TY - JOUR AU - Evans, CJ AU - Liu, T AU - Banerjee, U TI - Drosophila hematopoiesis: Markers and methods for molecular genetic analysis JF - METHODS J2 - METHODS VL - 68 PY - 2014 IS - 1 SP - 242 EP - 251 PG - 10 SN - 1046-2023 DO - 10.1016/j.ymeth.2014.02.038 UR - https://m2.mtmt.hu/api/publication/24399547 ID - 24399547 N1 - Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, United States Department of Biological Chemistry, University of California, Los Angeles, CA 90095, United States Molecular Biology Institute, University of California, Los Angeles, CA 90095, United States Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, United States Cited By :49 Export Date: 19 January 2022 CODEN: MTHDE Correspondence Address: Banerjee, U.; Department of Molecular, , Los Angeles, CA 90095, United States; email: banerjee@mbi.ucla.edu LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Csordás, Gábor AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Andó, István TI - The cell-mediated immunity of Drosophila melanogaster: Hemocyte lineages, immune compartments, microanatomy and regulation. JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 42 PY - 2014 IS - 1 SP - 47 EP - 56 PG - 10 SN - 0145-305X DO - 10.1016/j.dci.2013.06.005 UR - https://m2.mtmt.hu/api/publication/2372553 ID - 2372553 N1 - Cited By :124 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Andó, I.; Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary; email: ando.istvan@brc.mta.hu AB - In the animal kingdom, innate immunity is the first line of defense against invading pathogens. The dangers of microbial and parasitic attacks are countered by similar mechanisms, involving the prototypes of the cell-mediated immune responses, the phagocytosis and encapsulation. Work on Drosophila has played an important role in promoting an understanding of the basic mechanisms of phylogenetically conserved modules of innate immunity. The aim of this review is to survey the developments in the identification and functional definition of immune cell types and the immunological compartments of Drosophila melanogaster. We focus on the molecular and developmental aspects of the blood cell types and compartments, as well as the dynamics of blood cell development and the immune response. Further advances in the characterization of the innate immune mechanisms in Drosophila will provide basic clues to the understanding of the importance of the evolutionary conserved mechanisms of innate immune defenses in the animal kingdom. LA - English DB - MTMT ER - TY - JOUR AU - Kacsoh, BZ AU - Bozler, J AU - Schlenke, TA TI - A role for nematocytes in the cellular immune response of the Drosophilid Zaprionus indianus JF - PARASITOLOGY J2 - PARASITOLOGY VL - 141 PY - 2014 IS - 5 SP - 697 EP - 715 PG - 19 SN - 0031-1820 DO - 10.1017/S0031182013001431 UR - https://m2.mtmt.hu/api/publication/24783909 ID - 24783909 N1 - Cited By :13 Export Date: 19 January 2022 CODEN: PARAA Correspondence Address: Schlenke, T.A.; Biology Department, 1510 Clifton Road NE, United States; email: tschlen@emory.edu LA - English DB - MTMT ER - TY - JOUR AU - Sampson, Christopher J AU - Amin, Unum AU - Couso, Juan-Pablo TI - Activation of Drosophila hemocyte motility by the ecdysone hormone JF - BIOLOGY OPEN J2 - BIOL OPEN VL - 2 PY - 2013 IS - 12 SP - 1412 EP - 1420 PG - 9 SN - 2046-6390 DO - 10.1242/bio.20136619 UR - https://m2.mtmt.hu/api/publication/24854315 ID - 24854315 N1 - Cited By :17 Export Date: 12 February 2021 Correspondence Address: Couso, J.-P.; JMS Building, United Kingdom; email: J.P.Couso@sussex.ac.uk LA - English DB - MTMT ER - TY - JOUR AU - Zsámboki, János AU - Csordás, Gábor AU - Honti, Viktor AU - Pintér, Lajos AU - Bajusz, Izabella AU - Galgóczi, László Norbert AU - Andó, István AU - Kurucz, Judit Éva TI - Drosophila Nimrod proteins bind bacteria JF - CENTRAL EUROPEAN JOURNAL OF BIOLOGY J2 - CENT EUR J BIOL VL - 8 PY - 2013 IS - 7 SP - 633 EP - 645 PG - 13 SN - 1895-104X DO - 10.2478/s11535-013-0183-4 UR - https://m2.mtmt.hu/api/publication/2326115 ID - 2326115 N1 - Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, H-6726 Szeged, Hungary Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary Cited By :14 Export Date: 19 January 2022 Correspondence Address: Kurucz, É.; Institute of Genetics, , H-6726 Szeged, Hungary; email: kurucz.eva@brc.mta.hu AB - Engulfment of foreign particles by phagocytes is initiated by the engagement of phagocytic receptors. We have previously reported that NimC1 is involved in the phagocytosis of bacteria in Drosophila melanogaster. We have identified a family of genes, the Nimrod gene superfamily, encoding characteristic NIM domain containing structural homologues of NimC1. In this work we studied the bacterium-binding properties of the Nimrod proteins by using a novel immunofluorescencebased flow cytometric assay. This method proved to be highly reproducible and suitable for investigations of the bacteriumbinding capacities of putative phagocytosis receptors. We found that NimC1, NimA, NimB1 and NimB2 bind bacteria significantly but differently. In this respect they are similar to other NIM domain containing receptors Eater and Draper. LA - English DB - MTMT ER - TY - JOUR AU - Howell, L AU - Sampson, CJ AU - Xavier, MJ AU - Bolukbasi, E AU - Heck, MMS AU - Williams, MJ TI - A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response JF - IMMUNOGENETICS J2 - IMMUNOGENETICS VL - 64 PY - 2012 IS - 2 SP - 155 EP - 161 PG - 7 SN - 0093-7711 DO - 10.1007/s00251-011-0571-3 UR - https://m2.mtmt.hu/api/publication/22865290 ID - 22865290 N1 - Institute of Biological and Environmental Sciences, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ, United Kingdom Queen's Medical Research Institute, Centre for Cardiovascular Science, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, United Kingdom UCL Institute of Healthy Ageing Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden Cited By :22 Export Date: 19 January 2022 CODEN: IMNGB Correspondence Address: Williams, M.J.; Department of Neuroscience, Functional Pharmacology, , Uppsala, Sweden; email: michael.williams@neuro.uu.se LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Kurucz, Judit Éva AU - Jankovics, Ferenc AU - Andó, István TI - Cell lineage tracing reveals the plasticity of the hemocyte lineages and of the hematopoietic compartments in drosophila melanogaster JF - MOLECULAR IMMUNOLOGY J2 - MOL IMMUNOL VL - 47 PY - 2010 IS - 11-12 SP - 1997 EP - 2004 PG - 8 SN - 0161-5890 DO - 10.1016/j.molimm.2010.04.017 UR - https://m2.mtmt.hu/api/publication/1921048 ID - 1921048 N1 - Cited By :83 Export Date: 19 January 2022 CODEN: IMCHA Correspondence Address: Andó, I.; Institute of Genetics, Temesvári krt. 62, 6726 Szeged, Csongrád, Hungary; email: ando@brc.hu LA - English DB - MTMT ER -