TY - JOUR AU - Coleman-Gosser, Nikki AU - Hu, Yanhui AU - Raghuvanshi, Shiva AU - Stitzinger, Shane AU - Chen, Weihang AU - Luhur, Arthur AU - Mariyappa, Daniel AU - Josifov, Molly AU - Zelhof, Andrew AU - Mohr, Stephanie E. AU - Perrimon, Norbert AU - Simcox, Amanda TI - Continuous muscle, glial, epithelial, neuronal, and hemocyte cell lines for Drosophila research JF - ELIFE J2 - ELIFE VL - 12 PY - 2023 PG - 27 SN - 2050-084X DO - 10.7554/eLife.85814 UR - https://m2.mtmt.hu/api/publication/34261233 ID - 34261233 N1 - Export Date: 28 November 2023 AB - Expression of activated Ras, Ras(V12), provides Drosophila cultured cells with a proliferation and survival advantage that simplifies the generation of continuous cell lines. Here, we used lineage-restricted Ras(V12) expression to generate continuous cell lines of muscle, glial, and epithelial cell type. Additionally, cell lines with neuronal and hemocyte characteristics were isolated by cloning from cell cultures established with broad Ras(V12) expression. Differentiation with the hormone ecdysone caused maturation of cells from mesoderm lines into active muscle tissue and enhanced dendritic features in neuronal-like lines. Transcriptome analysis showed expression of key cell-type-specific genes and the expected alignment with single-cell sequencing and in situ data. Overall, the technique has produced in vitro cell models with characteristics of glia, epithelium, muscle, nerve, and hemocyte. The cells and associated data are available from the Drosophila Genomic Resource Center. LA - English DB - MTMT ER - TY - JOUR AU - Heron, R. AU - Amato, C. AU - Wood, W. AU - Davidson, A.J. TI - Understanding the diversity and dynamics of in vivo efferocytosis: Insights from the fly embryo JF - IMMUNOLOGICAL REVIEWS J2 - IMMUNOL REV PY - 2023 SN - 0105-2896 DO - 10.1111/imr.13266 UR - https://m2.mtmt.hu/api/publication/34132755 ID - 34132755 N1 - Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, United Kingdom School of Cancer Sciences, Wolfson Wohl Cancer Research Centre, University of Glasgow, Glasgow, United Kingdom Export Date: 8 September 2023 CODEN: IMRED Correspondence Address: Wood, W.; Institute for Regeneration and Repair, 5 Little France Drive, United Kingdom; email: w.wood@ed.ac.uk Correspondence Address: Davidson, A.J.; Institute for Regeneration and Repair, United Kingdom; email: andrew.davidson@glasgow.ac.uk LA - English DB - MTMT ER - TY - JOUR AU - Kúthy-Sutus, Enikő AU - Kharrat, Bayan AU - Gábor, Erika AU - Csordás, Gábor AU - Sinka, Rita AU - Honti, Viktor TI - A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 1 PG - 15 SN - 2073-4409 DO - 10.3390/cells12010024 UR - https://m2.mtmt.hu/api/publication/33555087 ID - 33555087 N1 - Export Date: 24 January 2023 AB - The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo. LA - English DB - MTMT ER - TY - JOUR AU - Quicray, M. AU - Wilhelm, L. AU - Enriquez, T. AU - He, S. AU - Scheifler, M. AU - Visser, B. TI - The Drosophila-parasitizing wasp Leptopilina heterotoma: A comprehensive model system in ecology and evolution JF - ECOLOGY AND EVOLUTION J2 - ECOL EVOL VL - 13 PY - 2023 IS - 1 SN - 2045-7758 DO - 10.1002/ece3.9625 UR - https://m2.mtmt.hu/api/publication/33628955 ID - 33628955 N1 - Export Date: 8 February 2023 AB - The parasitoid Leptopilina heterotoma has been used as a model system for more than 70 years, contributing greatly to diverse research areas in ecology and evolution. Here, we synthesized the large body of work on L. heterotoma with the aim to identify new research avenues that could be of interest also for researchers studying other parasitoids and insects. We start our review with a description of typical L. heterotoma characteristics, as well as that of the higher taxonomic groups to which this species belongs. We then continue discussing host suitability and immunity, foraging behaviors, as well as fat accumulation and life histories. We subsequently shift our focus towards parasitoid-parasitoid interactions, including L. heterotoma coexistence within the larger guild of Drosophila parasitoids, chemical communication, as well as mating and population structuring. We conclude our review by highlighting the assets of L. heterotoma as a model system, including its intermediate life history syndromes, the ease of observing and collecting natural hosts and wasps, as well as recent genomic advances. © 2023 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Magyar, Lilla Brigitta AU - Kovács, Attila Lajos AU - Lerner, Zita AU - Juhász, Gábor AU - Lukacsovich, David AU - Winterer, Jochen AU - Lukacsovich, Tamás AU - Hegedűs, Zoltán AU - Kurucz, Judit Éva AU - Hultmark, Dan AU - Földy, Csaba AU - Andó, István TI - Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 14 PY - 2022 IS - 4 SP - 335 EP - 354 PG - 20 SN - 1662-811X DO - 10.1159/000520110 UR - https://m2.mtmt.hu/api/publication/32524824 ID - 32524824 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Hultmark, Dan AU - Andó, István TI - Hematopoietic plasticity mapped in Drosophila and other insects JF - ELIFE J2 - ELIFE VL - 11 PY - 2022 PG - 36 SN - 2050-084X DO - 10.7554/eLife.78906 UR - https://m2.mtmt.hu/api/publication/33039275 ID - 33039275 N1 - Funding Agency and Grant Number: Vetenskapsradet [2018-05114]; Hungarian Science Foundation [K135877] Funding text: Vetenskapsradet 2018-05114 Dan HultmarkHungarian Science Foundation K135877 Istvan AndoThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. LA - English DB - MTMT ER - TY - JOUR AU - Koranteng, F. AU - Cho, B. AU - Shim, J. TI - Intrinsic and Extrinsic Regulation of Hematopoiesis in Drosophila JF - MOLECULES AND CELLS J2 - MOL CELLS VL - 45 PY - 2022 IS - 3 SP - 101 EP - 108 PG - 8 SN - 1016-8478 DO - 10.14348/molcells.2022.2039 UR - https://m2.mtmt.hu/api/publication/32876726 ID - 32876726 N1 - Export Date: 14 June 2022 CODEN: MOCEE AB - Drosophila melanogaster lymph gland, the primary site of hematopoiesis, contains myeloid-like progenitor cells that differentiate into functional hemocytes in the circulation of pupae and adults. Fly hemocytes are dynamic and plastic, and they play diverse roles in the innate immune response and wound healing. Various hematopoietic regulators in the lymph gland ensure the developmental and functional balance between progenitors and mature blood cells. In addition, systemic factors, such as nutrient availability and sensory inputs, integrate environmental variabilities to synchronize the blood development in the lymph gland with larval growth, physiology, and immunity. This review examines the intrinsic and extrinsic factors determining the progenitor states during hemocyte development in the lymph gland and provides new insights for further studies that may extend the frontier of our collective knowledge on hematopoiesis and innate immunity. © 2022, Korean Society for Molecular and Cellular Biology. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Vogel, M. AU - Shah, P. N. AU - Voulgari-Kokota, A. AU - Maistrou, S. AU - Aartsma, Y. AU - Beukeboom, L. W. AU - Salles, J. Falcao AU - Van Loon, J. J. A. AU - Dicke, M. AU - Wertheim, B. TI - Health of the black soldier fly and house fly under mass-rearing conditions: innate immunity and the role of the microbiome JF - Journal of Insects as Food and Feed J2 - J INSECTS FOOD FEED VL - 8 PY - 2022 IS - 8 SP - 857 EP - 878 PG - 22 SN - 2352-4588 DO - 10.3920/JIFF2021.0151 UR - https://m2.mtmt.hu/api/publication/33181589 ID - 33181589 N1 - Cited By :6 Export Date: 11 May 2023 AB - Rearing insects for food and feed is a rapidly growing industry, because it provides excellent opportunities for a sustainable approach to animal protein production. Two fly species, the black soldier fly (BSF) and the house fly (HF), naturally live in decaying organic matter (e.g. compost), and can thus be effectively reared on organic rest streams from the food and agricultural industry. The adoption of these insects as mini-livestock on microbially rich substrates, however, requires us to address how we can safeguard insect health under mass-rearing conditions. In this review, we discuss what is known about the innate immunity of insects in general, especially focusing on a comparative approach to current knowledge for the two dipteran species BSF and HE. We also discuss environmental factors that may affect innate immunity in mass-rearing settings, including temperature, insect densities and diet composition. Furthermore, we address the role of the microbiome in insect health and the associations of these fly species with detrimental or beneficial microbes. Finally, we present a perspective on important open scientific questions for optimizing the mass rearing of these insects with respect to their health and welfare. LA - English DB - MTMT ER - TY - JOUR AU - Wertheim, B. TI - Adaptations and counter-adaptations in Drosophila host–parasitoid interactions: advances in the molecular mechanisms JF - CURRENT OPINION IN INSECT SCIENCE J2 - CURR OPIN INSECT SCI VL - 51 PY - 2022 SN - 2214-5745 DO - 10.1016/j.cois.2022.100896 UR - https://m2.mtmt.hu/api/publication/32876692 ID - 32876692 N1 - Export Date: 14 June 2022 AB - Both hosts and parasitoids evolved a diverse array of traits and strategies for their antagonistic interactions, affecting their chances of encounter, attack and survival after parasitoid attack. This review summarizes the recent progress that has been made in elucidating the molecular mechanisms of these adaptations and counter-adaptations in various Drosophila host–parasitoid interactions. For the hosts, it focuses on the neurobiological and genetic control of strategies in Drosophila adults and larvae of avoidance or escape behaviours upon sensing the parasitoids, and the immunological defences involving diverse classes of haemocytes. For the parasitoids, it highlights their behavioural strategies in host finding, as well as the rich variety of venom components that evolved and were partially acquired through horizontal gene transfer. Recent studies revealed the mechanisms by which these venom components manipulate their parasitized hosts in exhibiting escape behaviour to avoid superparasitism, and their counter-strategies to evade or obstruct the hosts’ immunological defences. © 2022 The Author(s) LA - English DB - MTMT ER - TY - JOUR AU - Wu, Xiaotong AU - Wu, Zhiwei AU - Ye, Xiqian AU - Pang, Lan AU - Sheng, Yifeng AU - Wang, Zehua AU - Zhou, Yuenan AU - Zhu, Jiachen AU - Hu, Rongmin AU - Zhou, Sicong AU - Chen, Jiani AU - Wang, Zhizhi AU - Shi, Min AU - Huang, Jianhua AU - Chen, Xuexin TI - The Dual Functions of a Bracovirus C-Type Lectin in Caterpillar Immune Response Manipulation JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 13 PY - 2022 PG - 16 SN - 1664-3224 DO - 10.3389/fimmu.2022.877027 UR - https://m2.mtmt.hu/api/publication/32972124 ID - 32972124 N1 - Funding Agency and Grant Number: Key Project of Laboratory of Lingnan Modern Agriculture [NT2021003]; Key Program of National Natural Science Foundation of China [31630060]; National Key Research and Development Program of China [2019YFD0300104]; National Science Fund for Excellent Young Scholars [31622048]; National Science Foundation of China [32172467, 31672079]; Zhejiang Provincial Natural Science Foundation [LR18C140001] Funding text: This work was jointly supported by the Key Project of Laboratory of Lingnan Modern Agriculture (NT2021003), Key Program of National Natural Science Foundation of China (31630060), National Key Research and Development Program of China (2019YFD0300104) to XC, the National Science Fund for Excellent Young Scholars (31622048), and the National Science Foundation of China (32172467) to JH, and the National Science Foundation of China (31672079), and Zhejiang Provincial Natural Science Foundation (LR18C140001) to MS. AB - Parasitoids are widespread in natural ecosystems and normally equipped with diverse viral factors to defeat host immune responses. On the other hand, parasitoids can enhance the antibacterial abilities and improve the hypoimmunity traits of parasitized hosts that may encounter pathogenic infections. These adaptive strategies guarantee the survival of parasitoid offspring, yet their underlying mechanisms are poorly understood. Here, we focused on Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella, and found that C. vestalis parasitization decreases the number of host hemocytes, leading to disruption of the encapsulation reaction. We further found that one bracovirus C-type lectin gene, CvBV_28-1, is highly expressed in the hemocytes of parasitized hosts and participates in suppressing the proliferation rate of host hemocytes, which in turn reduces their population and represses the process of encapsulation. Moreover, CvBV_28-1 presents a classical bacterial clearance ability via the agglutination response in a Ca2+-dependent manner in response to gram-positive bacteria. Our study provides insights into the innovative strategy of a parasitoid-derived viral gene that has dual functions to manipulate host immunity for a successful parasitism. LA - English DB - MTMT ER - TY - JOUR AU - Yan, Yan AU - Sigle, Leah T. AU - Rinker, David C. AU - Estevez-Lao, Tania Y. AU - Capra, John A. AU - Hillyer, Julian F. TI - The immune deficiency and c-Jun N-terminal kinase pathways drive the functional integration of the immune and circulatory systems of mosquitoes JF - OPEN BIOLOGY J2 - OPEN BIOL VL - 12 PY - 2022 IS - 9 PG - 14 SN - 2046-2441 DO - 10.1098/rsob.220111 UR - https://m2.mtmt.hu/api/publication/33181490 ID - 33181490 AB - The immune and circulatory systems of animals are functionally integrated. In mammals, the spleen and lymph nodes filter and destroy microbes circulating in the blood and lymph, respectively. In insects, immune cells that surround the heart valves (ostia), called periostial haemocytes, destroy pathogens in the areas of the body that experience the swiftest haemolymph (blood) flow. An infection recruits additional periostial haemocytes, amplifying heart-associated immune responses. Although the structural mechanics of periostial haemocyte aggregation have been defined, the genetic factors that regulate this process remain less understood. Here, we conducted RNA sequencing in the African malaria mosquito, Anopheles gainhiac, and discovered that an infection upregulates multiple components of the immune deficiency (IMD) and c-Jun N-terminal kinase (JNK) pathways in the heart with periostial haemo cytes. This upregulahon is greater in the heart with periostial haemocytes than in the circulating haemocytes or the entire abdomen. RNA interference-based knockdown then showed that the IMD and JNK pathways drive periostial haemocyte aggregation and alter phagocytosis and melanization on the heart, thereby demonstrating that these pathways regulate the functional integration between the immune and circulatory systems. Understanding how insects fight infection lays the foundation for novel strategies that could protect beneficial insects and harm detrimental ones. LA - English DB - MTMT ER - TY - JOUR AU - Yu, S. AU - Luo, F. AU - Xu, Y. AU - Zhang, Y. AU - Jin, L.H. TI - Drosophila Innate Immunity Involves Multiple Signaling Pathways and Coordinated Communication Between Different Tissues JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 13 PY - 2022 PG - 23 SN - 1664-3224 DO - 10.3389/fimmu.2022.905370 UR - https://m2.mtmt.hu/api/publication/33041863 ID - 33041863 N1 - Export Date: 8 August 2022 AB - The innate immune response provides the first line of defense against invading pathogens, and immune disorders cause a variety of diseases. The fruit fly Drosophila melanogaster employs multiple innate immune reactions to resist infection. First, epithelial tissues function as physical barriers to prevent pathogen invasion. In addition, macrophage-like plasmatocytes eliminate intruders through phagocytosis, and lamellocytes encapsulate large particles, such as wasp eggs, that cannot be phagocytosed. Regarding humoral immune responses, the fat body, equivalent to the mammalian liver, secretes antimicrobial peptides into hemolymph, killing bacteria and fungi. Drosophila has been shown to be a powerful in vivo model for studying the mechanism of innate immunity and host-pathogen interactions because Drosophila and higher organisms share conserved signaling pathways and factors. Moreover, the ease with which Drosophila genetic and physiological characteristics can be manipulated prevents interference by adaptive immunity. In this review, we discuss the signaling pathways activated in Drosophila innate immunity, namely, the Toll, Imd, JNK, JAK/STAT pathways, and other factors, as well as relevant regulatory networks. We also review the mechanisms by which different tissues, including hemocytes, the fat body, the lymph gland, muscles, the gut and the brain coordinate innate immune responses. Furthermore, the latest studies in this field are outlined in this review. In summary, understanding the mechanism underlying innate immunity orchestration in Drosophila will help us better study human innate immunity-related diseases. Copyright © 2022 Yu, Luo, Xu, Zhang and Jin. LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Gábor, Erika AU - Honti, Viktor TI - There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 469 PY - 2021 SP - 135 EP - 143 PG - 9 SN - 0012-1606 DO - 10.1016/j.ydbio.2020.10.006 UR - https://m2.mtmt.hu/api/publication/31743832 ID - 31743832 N1 - Cited By :5 Export Date: 14 June 2022 CODEN: DEBIA LA - English DB - MTMT ER - TY - JOUR AU - Eleftherianos, Ioannis AU - Heryanto, Christa AU - Bassal, Taha AU - Zhang, Wei AU - Tettamanti, Gianluca AU - Mohamed, Amr TI - Haemocyte-mediated immunity in insects: Cells, processes and associated components in the fight against pathogens and parasites JF - IMMUNOLOGY J2 - IMMUNOLOGY VL - 164 PY - 2021 IS - 3 SP - 401 EP - 432 PG - 32 SN - 0019-2805 DO - 10.1111/imm.13390 UR - https://m2.mtmt.hu/api/publication/32361576 ID - 32361576 N1 - Infection and Innate Immunity Laboratory, Department of Biological Sciences, Institute for Biomedical Sciences, The George Washington University, Washington, DC, United States Department of Entomology, Faculty of Science, Cairo University, Giza, Egypt State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Napoli Federico II, Napoli, Italy Cited By :4 Export Date: 19 January 2022 CODEN: IMMUA Correspondence Address: Eleftherianos, I.; Infection and Innate Immunity Laboratory, United States; email: ioannise@gwu.edu Correspondence Address: Mohamed, A.; Department of Entomology, Egypt; email: mamr@sci.cu.edu.eg AB - The host defence of insects includes a combination of cellular and humoral responses. The cellular arm of the insect innate immune system includes mechanisms that are directly mediated by haemocytes (e.g., phagocytosis, nodulation and encapsulation). In addition, melanization accompanying coagulation, clot formation and wound healing, nodulation and encapsulation processes leads to the formation of cytotoxic redox-cycling melanin precursors and reactive oxygen and nitrogen species. However, demarcation between cellular and humoral immune reactions as two distinct categories is not straightforward. This is because many humoral factors affect haemocyte functions and haemocytes themselves are an important source of many humoral molecules. There is also a considerable overlap between cellular and humoral immune functions that span from recognition of foreign intruders to clot formation. Here, we review these immune reactions starting with the cellular mechanisms that limit haemolymph loss and participate in wound healing and clot formation and advancing to cellular functions that are critical in restricting pathogen movement and replication. This information is important because it highlights that insect cellular immunity is controlled by a multilayered system, different components of which are activated by different pathogens or during the different stages of the infection. LA - English DB - MTMT ER - TY - JOUR AU - Evans, Cory J. AU - Olson, John M. AU - Mondal, Bama Charan AU - Kandimalla, Pratyush AU - Abbasi, Ariano AU - Abdusamad, Mai M. AU - Acosta, Osvaldo AU - Ainsworth, Julia A. AU - Akram, Haris M. AU - Albert, Ralph B. AU - Alegria-Leal, Elitzander AU - Alexander, Kai Y. AU - Ayala, Angelica C. AU - Balashova, Nataliya S. AU - Barber, Rebecca M. AU - Bassi, Harmanjit AU - Bennion, Sean P. AU - Beyder, Miriam AU - Bhatt, Kush V AU - Bhoot, Chinmay AU - Bradshaw, Aaron W. AU - Brannigan, Tierney G. AU - Cao, Boyu AU - Cashell, Yancey Y. AU - Chai, Timothy AU - Chan, Alex W. AU - Chan, Carissa AU - Chang, Inho AU - Chang, Jonathan AU - Chang, Michael T. AU - Chang, Patrick W. AU - Chang, Stephen AU - Chari, Neel AU - Chassiakos, Alexander J. AU - Chen, Iris E. AU - Chen, Vivian K. AU - Chen, Zheying AU - Cheng, Marsha R. AU - Chiang, Mimi AU - Chiu, Vivian AU - Choi, Sharon AU - Chung, Jun Ho AU - Contreras, Liset AU - Corona, Edgar AU - Cruz, Courtney J. AU - Cruz, Renae L. AU - Dang, Jefferson M. AU - Dasari, Suhas P. AU - De la Fuente, Justin R. O. AU - Del Rio, Oscar M. A. AU - Dennis, Emily R. AU - Dertsakyan, Petros S. AU - Dey, Ipsita AU - Distler, Rachel S. AU - Dong, Zhiqiao AU - Dorman, Leah C. AU - Douglass, Mark A. AU - Ehresman, Allysen B. AU - Fu, Ivy H. AU - Fua, Andrea AU - Full, Sean M. AU - Ghaffari-Rafi, Arash AU - Ghani, Asmar Abdul AU - Giap, Bosco AU - Gill, Sonia AU - Gill, Zafar S. AU - Gills, Nicholas J. AU - Godavarthi, Sindhuja AU - Golnazarian, Talin AU - Goyal, Raghav AU - Gray, Ricardo AU - Grunfeld, Alexander M. AU - Gu, Kelly M. AU - Gutierrez, Natalia C. AU - Ha, An N. AU - Hamid, Iman AU - Hanson, Ashley AU - Hao, Celesti AU - He, Chongbin AU - He, Mengshi AU - Hedtke, Joshua P. AU - Hernandez, Ysrael K. AU - Hlaing, Hnin AU - Hobby, Faith A. AU - Hoi, Karen AU - Hope, Ashley C. AU - Hosseinian, Sahra M. AU - Hsu, Alice AU - Hsueh, Jennifer AU - Hu, Eileen AU - Hu, Spencer S. AU - Huang, Stephanie AU - Huang, Wilson AU - Huynh, Melanie AU - Javier, Carmen AU - Jeon, Na Eun AU - Ji, Sunjong AU - Johal, Jasmin AU - John, Amala AU - Johnson, Lauren AU - Kadakia, Saurin AU - Kakade, Namrata AU - Kamel, Sarah AU - Kaur, Ravinder AU - Khatra, Jagteshwar S. AU - Kho, Jeffrey A. AU - Kim, Caleb AU - Kim, Emily Jin-Kyung AU - Kim, Hee Jong AU - Kim, Hyun Wook AU - Kim, Jin Hee AU - Kim, Seong Ah AU - Kim, Woo Kyeom AU - Kit, Brian AU - La, Cindy AU - Lai, Jonathan AU - Lam, Vivian AU - Nguyen Khoi Le, null AU - Lee, Chi Ju AU - Lee, Dana AU - Lee, Dong Yeon AU - Lee, James AU - Lee, Jason AU - Lee, Jessica AU - Lee, Ju-Yeon AU - Lee, Sharon AU - Lee, Terrence C. AU - Lee, Victoria AU - Li, Amber J. AU - Li, Jialing AU - Libro, Alexandra M. AU - Lien, Irvin C. AU - Lim, Mia AU - Lin, Jeffrey M. AU - Liu, Connie Y. AU - Liu, Steven C. AU - Louie, Irene AU - Lu, Shijia W. AU - Luo, William Y. AU - Luu, Tiffany AU - Madrigal, Josef T. AU - Mai, Yishan AU - Miya, Darron I AU - Mohammadi, Mina AU - Mohanta, Sayonika AU - Mokwena, Tebogo AU - Montoya, Tonatiuh AU - Mould, Dallas L. AU - Murata, Mark R. AU - Muthaiya, Janani AU - Naicker, Seethim AU - Neebe, Mallory R. AU - Ngo, Amy AU - Ngo, Duy Q. AU - Ngo, Jamie A. AU - Nguyen, Anh T. AU - Nguyen, Huy C. X. AU - Nguyen, Rina H. AU - Nguyen, Thao T. T. AU - Nguyen, Vincent T. AU - Nishida, Kevin AU - Oh, Seo-Kyung AU - Omi, Kristen M. AU - Onglatco, Mary C. AU - Almazan, Guadalupe Ortega AU - Paguntalan, Jahzeel AU - Panchal, Maharshi AU - Pang, Stephanie AU - Parikh, Harin B. AU - Patel, Purvi D. AU - Patel, Trisha H. AU - Petersen, Julia E. AU - Pham, Steven AU - Phan-Everson, Tien M. AU - Pokhriyal, Megha AU - Popovich, Davis W. AU - Quaal, Adam T. AU - Querubin, Karl AU - Resendiz, Anabel AU - Riabkova, Nadezhda AU - Rong, Fred AU - Salarkia, Sarah AU - Sama, Nateli AU - Sang, Elaine AU - Sanville, David A. AU - Schoen, Emily R. AU - Shen, Zhouyang AU - Siangchin, Ken AU - Sibal, Gabrielle AU - Sin, Garuem AU - Sjarif, Jasmine AU - Smith, Christopher J. AU - Soeboer, Annisa N. AU - Sosa, Cristian AU - Spitters, Derek AU - Stender, Bryan AU - Su, Chloe C. AU - Summapund, Jenny AU - Sun, Beatrice J. AU - Sutanto, Christine AU - Tan, Jaime S. AU - Tan, Nguon L. AU - Tangmatitam, Parich AU - Trac, Cindy K. AU - Tran, Conny AU - Tran, Daniel AU - Tran, Duy AU - Tran, Vina AU - Truong, Patrick A. AU - Tsai, Brandon L. AU - Tsai, Pei-Hua AU - Tsui, C. Kimberly AU - Uriu, Jackson K. AU - Venkatesh, Sanan AU - Vo, Maique AU - Nhat-Thi Vo, null AU - Phuong Vo, null AU - Voros, Timothy C. AU - Wan, Yuan AU - Wang, Eric AU - Wang, Jeffrey AU - Wang, Michael K. AU - Wang, Yuxuan AU - Wei, Siman AU - Wilson, Matthew N. AU - Wong, Daniel AU - Wu, Elliott AU - Xing, Hanning AU - Xu, Jason P. AU - Yaftaly, Sahar AU - Yan, Kimberly AU - Yang, Evan AU - Yang, Rebecca AU - Yao, Tony AU - Yeo, Patricia AU - Yip, Vivian AU - Yogi, Puja AU - Young, Gloria Chin AU - Yung, Maggie M. AU - Zai, Alexander AU - Zhang, Christine AU - Zhang, Xiao X. AU - Zhao, Zijun AU - Zhou, Raymond AU - Zhou, Ziqi AU - Abutouk, Mona AU - Aguirre, Brian AU - Ao, Chon AU - Baranoff, Alexis AU - Beniwal, Angad AU - Cai, Zijie AU - Chan, Ryan AU - Chien, Kenneth Chang AU - Chaudhary, Umar AU - Chin, Patrick AU - Chowdhury, Praptee AU - Dalie, Jamlah AU - Du, Eric Y. AU - Estrada, Alec AU - Feng, Erwin AU - Ghaly, Monica AU - Graf, Rose AU - Hernandez, Eduardo AU - Herrera, Kevin AU - Ho, Vivien W. AU - Honeychurch, Kaitlyn AU - Hou, Yurianna AU - Huang, Jo M. AU - Ishii, Momoko AU - James, Nicholas AU - Jang, Gah-Eun AU - Jin, Daphne AU - Juarez, Jesse AU - Kesaf, Ayse Elif AU - Khalsa, Sat Kartar AU - Kim, Hannah AU - Kovsky, Jenna AU - Kuang, Chak Lon AU - Kumar, Shraddha AU - Lam, Gloria AU - Lee, Ceejay AU - Lee, Grace AU - Li, Li AU - Lin, Joshua AU - Liu, Josephine AU - Ly, Janice AU - Ma, Austin AU - Markovic, Hannah AU - Medina, Cristian AU - Mungcal, Jonelle AU - Naranbaatar, Bilguudei AU - Patel, Kayla AU - Petersen, Lauren AU - Phan, Amanda AU - Phung, Malcolm AU - Priasti, Nadiyah AU - Ruano, Nancy AU - Salim, Tanveer AU - Schnell, Kristen AU - Shah, Paras AU - Shen, Jinhua AU - Stutzman, Nathan AU - Sukhina, Alisa AU - Tian, Rayna AU - Vega-Loza, Andrea AU - Wang, Joyce AU - Wang, Jun AU - Watanabe, Rina AU - Wei, Brandon AU - Xie, Lillian AU - Ye, Jessica AU - Zhao, Jeffrey AU - Zimmerman, Jill AU - Bracken, Colton AU - Capili, Jason AU - Char, Andrew AU - Chen, Michel AU - Huang, Pingdi AU - Ji, Sena AU - Kim, Emily AU - Kim, Kenneth AU - Ko, Julie AU - Laput, Sean Louise G. AU - Law, Sam AU - Lee, Sang Kuk AU - Lee, Olivia AU - Lim, David AU - Lin, Eric AU - Marik, Kyle AU - Mytych, Josh AU - O'Laughlin, Andie AU - Pak, Jensen AU - Park, Claire AU - Ryu, Ruth AU - Shinde, Ashwin AU - Sosa, Manny AU - Waite, Nick AU - Williams, Mane AU - Wong, Richard AU - Woo, Jocelyn AU - Woo, Jonathan AU - Yepuri, Vishaal AU - Yim, Dorothy AU - Dan Huynh, null AU - Wijiewarnasurya, Dinali AU - Shapiro, Casey AU - Levis-Fitzgerald, Marc AU - Jaworski, Leslie AU - Lopatto, David AU - Clark, Ira E. AU - Johnson, Tracy AU - Banerjee, Utpal TI - A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 11 PY - 2021 IS - 1 PG - 23 SN - 2160-1836 DO - 10.1093/g3journal/jkaa028 UR - https://m2.mtmt.hu/api/publication/32362075 ID - 32362075 N1 - Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, United States Biomedical Research Minor, University of California, Los Angeles, Los Angeles, CA 90095, United States Center for the Advancement of Teaching, University of California, Los Angeles, Los Angeles, CA 90095, United States Department of Psychology, Grinnell College, Grinnell, IA 50112, United States Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, United States UCLA Broad Stem Cell Research Center, University of California, Los Angeles, Los Angeles, CA 90095, United States Department of Biology, Loyola Marymount University, Los Angeles, CA, United States DNA Learning Center, Cold Spring Harbor Asia, Suzhou, Jiangsu, China Cytogenetics Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India Export Date: 19 January 2022 Correspondence Address: Evans, C.J.; Department of Biology, 1 LMU Drive, United States; email: Cory.Evans@lmu.edu Correspondence Address: Banerjee, U.; Department of Molecular, United States; email: banerjee@mbi.ucla.edu AB - Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach. LA - English DB - MTMT ER - TY - JOUR AU - Herbath, M. AU - Fabry, Z. AU - Sandor, M. TI - Current concepts in granulomatous immune responses JF - BIOLOGIA FUTURA J2 - BIOL FUTURA VL - 72 PY - 2021 IS - 1 SP - 61 EP - 68 PG - 8 SN - 2676-8615 DO - 10.1007/s42977-021-00077-1 UR - https://m2.mtmt.hu/api/publication/32000172 ID - 32000172 N1 - Export Date: 5 May 2021 Correspondence Address: Herbath, M.; Department of Pathology and Laboratory Medicine, United States; email: herbath@wisc.edu Export Date: 10 May 2021 Correspondence Address: Herbath, M.; Department of Pathology and Laboratory Medicine, United States; email: herbath@wisc.edu AB - Persistent irritants that are resistant to innate and cognate immunity induce granulomas. These macrophage-dominated lesions that partially isolate the healthy tissue from the irritant and the irritant induced inflammation. Particles, toxins, autoantigens and infectious agents can induce granulomas. The corresponding lesions can be protective for the host but they can also cause damage and such damage has been associated with the pathology of more than a hundred human diseases. Recently, multiple molecular mechanisms underlying how normal macrophages transform into granuloma-inducing macrophages have been discovered and new information has been gathered, indicating how these lesions are initiated, spread and regulated. In this review, differences between the innate and cognate granuloma pathways are discussed by summarizing how the dendritic cell-T cell axis changes granulomatous immunity. Granuloma lesions are highly dynamic and depend on continuous cell replacement. This feature provides new therapeutic approaches to treat granulomatous diseases. © 2021, Akadémiai Kiadó Zrt. LA - English DB - MTMT ER - TY - JOUR AU - Järvelä-Stölting, M. AU - Vesala, L. AU - Maasdorp, M.K. AU - Ciantar, J. AU - Rämet, M. AU - Valanne, S. TI - Proteasome α6 Subunit Negatively Regulates the JAK/STAT Pathway and Blood Cell Activation in Drosophila melanogaster JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 SN - 1664-3224 DO - 10.3389/fimmu.2021.729631 UR - https://m2.mtmt.hu/api/publication/32587143 ID - 32587143 N1 - Laboratory of Experimental Immunology, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland Research Unit for Pediatrics, Pediatric Neurology, Pediatric Surgery, Child Psychiatry, Dermatology, Clinical Genetics, Obstetrics and Gynecology, Otorhinolaryngology and Ophthalmology, Faculty of Medicine, University of Oulu, Oulu, Finland Medical Research Center Oulu, University of Oulu, Oulu, Finland Department of Children and Adolescents, Oulu University Hospital, University of Oulu, Oulu, Finland Export Date: 11 January 2022 Correspondence Address: Valanne, S.; Laboratory of Experimental Immunology, Finland; email: susanna.valanne@tuni.fi AB - JAK/STAT signaling regulates central biological functions such as development, cell differentiation and immune responses. In Drosophila, misregulated JAK/STAT signaling in blood cells (hemocytes) induces their aberrant activation. Using mass spectrometry to analyze proteins associated with a negative regulator of the JAK/STAT pathway, and by performing a genome-wide RNAi screen, we identified several components of the proteasome complex as negative regulators of JAK/STAT signaling in Drosophila. A selected proteasome component, Prosα6, was studied further. In S2 cells, Prosα6 silencing decreased the amount of the known negative regulator of the pathway, ET, leading to enhanced expression of a JAK/STAT pathway reporter gene. Silencing of Prosα6 in vivo resulted in activation of the JAK/STAT pathway, leading to the formation of lamellocytes, a specific hemocyte type indicative of hemocyte activation. This hemocyte phenotype could be partially rescued by simultaneous knockdown of either the Drosophila STAT transcription factor, or MAPKK in the JNK-pathway. Our results suggest a role for the proteasome complex components in the JAK/STAT pathway in Drosophila blood cells both in vitro and in vivo. Copyright © 2021 Järvelä-Stölting, Vesala, Maasdorp, Ciantar, Rämet and Valanne. LA - English DB - MTMT ER - TY - JOUR AU - Khalili, Dilan AU - Kalcher, Christina AU - Baumgartner, Stefan AU - Theopold, Ulrich TI - Anti-Fibrotic Activity of an Antimicrobial Peptide in a Drosophila Model JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 13 PY - 2021 IS - 6 SP - 376 EP - 390 PG - 15 SN - 1662-811X DO - 10.1159/000516104 UR - https://m2.mtmt.hu/api/publication/32362077 ID - 32362077 N1 - Department of Molecular Biosciences, The Wenner-Gren Institute (MBW), Stockholm University, Stockholm, Sweden Department of Experimental Medical Sciences, Lund University, Lund, Sweden Cited By :1 Export Date: 19 January 2022 Correspondence Address: Theopold, U.; Department of Molecular Biosciences, Sweden; email: uli.theopold@su.se AB - Fibrotic lesions accompany several pathological conditions, including tumors. We show that expression of a dominant-active form of the Ras oncogene in Drosophila salivary glands (SGs) leads to redistribution of components of the basement membrane (BM) and fibrotic lesions. Similar to several types of mammalian fibrosis, the disturbed BM attracts clot components, including insect transglutaminase and phenoloxidase. SG epithelial cells show reduced apicobasal polarity accompanied by a loss of secretory activity. Both the fibrotic lesions and the reduced cell polarity are alleviated by ectopic expression of the antimicrobial peptide drosomycin (Drs), which also restores the secretory activity of the SGs. In addition to extracellular matrix components, both Drs and F-actin localize to fibrotic lesions. LA - English DB - MTMT ER - TY - JOUR AU - Mase, A. AU - Augsburger, J. AU - Brückner, K. TI - Macrophages and Their Organ Locations Shape Each Other in Development and Homeostasis – A Drosophila Perspective JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 9 PY - 2021 SN - 2296-634X DO - 10.3389/fcell.2021.630272 UR - https://m2.mtmt.hu/api/publication/32006156 ID - 32006156 N1 - Export Date: 10 May 2021 Correspondence Address: Brückner, K.; Department of Cell and Tissue Biology, United States; email: katja.brueckner@ucsf.edu Correspondence Address: Brückner, K.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, United States; email: katja.brueckner@ucsf.edu Correspondence Address: Brückner, K.; Cardiovascular Research Institute, United States; email: katja.brueckner@ucsf.edu AB - Across the animal kingdom, macrophages are known for their functions in innate immunity, but they also play key roles in development and homeostasis. Recent insights from single cell profiling and other approaches in the invertebrate model organism Drosophila melanogaster reveal substantial diversity among Drosophila macrophages (plasmatocytes). Together with vertebrate studies that show genuine expression signatures of macrophages based on their organ microenvironments, it is expected that Drosophila macrophage functional diversity is shaped by their anatomical locations and systemic conditions. In vivo evidence for diverse macrophage functions has already been well established by Drosophila genetics: Drosophila macrophages play key roles in various aspects of development and organogenesis, including embryogenesis and development of the nervous, digestive, and reproductive systems. Macrophages further maintain homeostasis in various organ systems and promote regeneration following organ damage and injury. The interdependence and interplay of tissues and their local macrophage populations in Drosophila have implications for understanding principles of organ development and homeostasis in a wide range of species. © Copyright © 2021 Mase, Augsburger and Brückner. LA - English DB - MTMT ER - TY - JOUR AU - Mishra, M. AU - Panda, M. TI - Reactive oxygen species: the root cause of nanoparticle-induced toxicity in Drosophila melanogaster JF - FREE RADICAL RESEARCH J2 - FREE RADIC RES VL - 55 PY - 2021 IS - 6 SP - 671 EP - 687 PG - 17 SN - 1071-5762 DO - 10.1080/10715762.2021.1914335 UR - https://m2.mtmt.hu/api/publication/32006124 ID - 32006124 N1 - Export Date: 10 May 2021 CODEN: FRARE Correspondence Address: Mishra, M.; Neural Developmental Biology Lab, India AB - Nanotechnology is a rapidly developing technology in the twenty-first century. Nanomaterials are extensively used in numerous industries including cosmetics, food, medicines, industries, agriculture, etc. Along with its wide application toxicity is also reported from studies of various model organisms including Drosophila. The toxicity reflects cytotoxicity, genotoxicity, and teratogenicity. The current study correlates the toxicity as a consequence of reactive oxygen species (ROS) generated owing to the presence of nanoparticles with the living cell. ROS mainly includes hydroxyl ions, peroxide ions, superoxide anions, singlet oxygen, and hypochlorous acids. An elevated level of ROS can damage the cells by various means. To protect the body from excess ROS, living cells possess a set of antioxidant enzymes which includes peroxidase, glutathione peroxidase, and catalase. If the antioxidant enzymes cannot nullify the elevated ROS level than DNA damage, cell damage, cytotoxicity, apoptosis, and uncontrolled cell regulations occur resulting in abnormal physiological and genotoxic conditions. Herewith, we are reporting various morphological and physiological defects caused after nanoparticle treatment as a function of redox imbalance. © 2021 Informa UK Limited, trading as Taylor & Francis Group. LA - English DB - MTMT ER - TY - JOUR AU - Morin-Poulard, Ismael AU - Tian, Yushun AU - Vanzo, Nathalie AU - Crozatier, Michele TI - Drosophila as a Model to Study Cellular Communication Between the Hematopoietic Niche and Blood Progenitors Under Homeostatic Conditions and in Response to an Immune Stress JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 PG - 11 SN - 1664-3224 DO - 10.3389/fimmu.2021.719349 UR - https://m2.mtmt.hu/api/publication/32361805 ID - 32361805 N1 - Export Date: 19 January 2022 Correspondence Address: Crozatier, M.; MCD, France AB - In adult mammals, blood cells are formed from hematopoietic stem progenitor cells, which are controlled by a complex cellular microenvironment called "niche". Drosophila melanogaster is a powerful model organism to decipher the mechanisms controlling hematopoiesis, due both to its limited number of blood cell lineages and to the conservation of genes and signaling pathways throughout bilaterian evolution. Insect blood cells or hemocytes are similar to the mammalian myeloid lineage that ensures innate immunity functions. Like in vertebrates, two waves of hematopoiesis occur in Drosophila. The first wave takes place during embryogenesis. The second wave occurs at larval stages, where two distinct hematopoietic sites are identified: subcuticular hematopoietic pockets and a specialized hematopoietic organ called the lymph gland. In both sites, hematopoiesis is regulated by distinct niches. In hematopoietic pockets, sensory neurons of the peripheral nervous system provide a microenvironment that promotes embryonic hemocyte expansion and differentiation. In the lymph gland blood cells are produced from hematopoietic progenitors. A small cluster of cells called Posterior Signaling Centre (PSC) and the vascular system, along which the lymph gland develops, act collectively as a niche, under homeostatic conditions, to control the balance between maintenance and differentiation of lymph gland progenitors. In response to an immune stress such as wasp parasitism, lymph gland hematopoiesis is drastically modified and shifts towards emergency hematopoiesis, leading to increased progenitor proliferation and their differentiation into lamellocyte, a specific blood cell type which will neutralize the parasite. The PSC is essential to control this emergency response. In this review, we summarize Drosophila cellular and molecular mechanisms involved in the communication between the niche and hematopoietic progenitors, both under homeostatic and stress conditions. Finally, we discuss similarities between mechanisms by which niches regulate hematopoietic stem/progenitor cells in Drosophila and mammals. LA - English DB - MTMT ER - TY - JOUR AU - Mortimer, Nathan T. AU - Fischer, Mary L. AU - Waring, Ashley L. AU - Pooja, K. R. AU - Kacsoh, Balint Z. AU - Brantley, Susanna E. AU - Keebaugh, Erin S. AU - Hill, Joshua AU - Lark, Chris AU - Martin, Julia AU - Bains, Pravleen AU - Lee, Jonathan AU - Vrailas-Mortimer, Alysia D. AU - Schlenke, Todd A. TI - Extracellular matrix protein N-glycosylation mediates immune self-tolerance in Drosophila melanogaster JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 118 PY - 2021 IS - 39 PG - 12 SN - 0027-8424 DO - 10.1073/pnas.2017460118|1of12 UR - https://m2.mtmt.hu/api/publication/34589425 ID - 34589425 N1 - Funding Agency and Grant Number: NIH [R35GM133760, R01AI081879]; NSF [DBI1828136]; Bloomington Drosophila Stock Center (NIH) [P40OD018537] Funding text: We thank two anonymous reviewers, as well as members of the N.T.M. and T.A.S. laboratories, for detailed and constructive feedback that greatly improved this manuscript. Research reported in this publication was supported by NIH Awards R35GM133760 to N.T.M. and R01AI081879 to T.A.S. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. The Illinois State University Confocal Microscopy Facility was funded by NSF Grant DBI1828136. AB - In order to respond to infection, hosts must distinguish pathogens from their own tissues. This allows for the precise targeting of immune responses against pathogens and also ensures self-tolerance, the ability of the host to protect self tissues from immune damage. One way to maintain self-tolerance is to evolve a self signal and suppress any immune response directed at tissues that carry this signal. Here, we characterize the Drosophila tuSz1 mutant strain, which mounts an aberrant immune response against its own fat body. We demonstrate that this autoimmunity is the result of two mutations: 1) a mutation in the GCS1 gene that disrupts N-glycosylation of extracellular matrix proteins covering the fat body, and 2) a mutation in the Drosophila Janus Kinase ortholog that causes precocious activation of hemocytes. Our data indicate that N-glycans attached to extracellular matrix proteins serve as a self signal and that activated hemocytes attack tissues lacking this signal. The simplicity of this invertebrate self-recognition system and the ubiquity of its constituent parts suggests it may have functional homologs across animals. LA - English DB - MTMT ER - TY - JOUR AU - Mortimer, N.T. AU - Fischer, M.L. AU - Waring, A.L. AU - Pooja, K.R. AU - Kacsoh, B.Z. AU - Brantley, S.E. AU - Keebaugh, E.S. AU - Hill, J. AU - Lark, C. AU - Martin, J. AU - Bains, P. AU - Lee, J. AU - Vrailas-Mortimer, A.D. AU - Schlenke, T.A. TI - Extracellular matrix protein N-glycosylation mediates immune self-tolerance in Drosophila melanogaster JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 118 PY - 2021 IS - 39 SN - 0027-8424 DO - 10.1073/pnas.2017460118 UR - https://m2.mtmt.hu/api/publication/32876736 ID - 32876736 N1 - Export Date: 14 June 2022 CODEN: PNASA AB - In order to respond to infection, hosts must distinguish pathogens from their own tissues. This allows for the precise targeting of immune responses against pathogens and also ensures self-tolerance, the ability of the host to protect self tissues from immune damage. One way to maintain self-tolerance is to evolve a self signal and suppress any immune response directed at tissues that carry this signal. Here, we characterize the Drosophila tuSz1 mutant strain, which mounts an aberrant immune response against its own fat body. We demonstrate that this autoimmunity is the result of two mutations: 1) a mutation in the GCS1 gene that disrupts N-glycosylation of extracellular matrix proteins covering the fat body, and 2) a mutation in the Drosophila Janus Kinase ortholog that causes precocious activation of hemocytes. Our data indicate that N-glycans attached to extracellular matrix proteins serve as a self signal and that activated hemocytes attack tissues lacking this signal. The simplicity of this invertebrate self-recognition system and the ubiquity of its constituent parts suggests it may have functional homologs across animals. © 2021 National Academy of Sciences. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Nanda, K.P. AU - Firdaus, H. TI - Dietary cadmium (Cd) reduces hemocyte level by induction of apoptosis in Drosophila melanogaster JF - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY AND PHARMACOLOGY J2 - COMP BIOCHEM PHYS C VL - 250 PY - 2021 SN - 1532-0456 DO - 10.1016/j.cbpc.2021.109188 UR - https://m2.mtmt.hu/api/publication/32601957 ID - 32601957 N1 - Export Date: 19 January 2022 CODEN: CBPPF Correspondence Address: Firdaus, H.; Department of Life Sciences, Ratu-Lohardaga Road, Brambe, India; email: hena.firdaus@cuj.ac.in AB - Drosophila melanogaster larvae ensure continuous proliferation and differentiation of hemocytes to maintain a fixed range of different blood cell types during its various stages of development. Variation in this number is often an indicator of animal well-being, its genotype or an effect of environmental perturbation, including exposure to heavy metals. The present study investigates the effect of Cd on larval hemocytes. Embryos were allowed to grow in metal media till third instar larvae and finally circulating hemocyte were collected. The number of major hemocytes, plasmatocytes and crystal cells was determined to be lowered in Cd exposed animals. Our results also showed modulation of antioxidant biology of Cd exposed hemocytes by changing the major antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) activity, and decreasing reduced glutathione (GSH) levels in hemocytes suspended in the hemolymph. Acridine orange (AO) staining further revealed induction of apoptosis in hemocytes of metal treated larvae. Our results suggest a negative impact of Cd exposure on the hemocytes of the Drosophila larvae culminating in their lowered count by induction of apoptosis. © 2021 Elsevier Inc. LA - English DB - MTMT ER - TY - JOUR AU - Spratford, C.M. AU - Goins, L.M. AU - Chi, F. AU - Girard, J.R. AU - Macias, S.N. AU - Ho, V.W. AU - Banerjee, U. TI - Intermediate progenitor cells provide a transition between hematopoietic progenitors and their differentiated descendants JF - DEVELOPMENT J2 - DEVELOPMENT VL - 148 PY - 2021 IS - 24 SN - 0950-1991 DO - 10.1242/dev.200216 UR - https://m2.mtmt.hu/api/publication/32601905 ID - 32601905 N1 - Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, United States Molecular Biology Institute, University of California, Los Angeles, United States Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, United States Department of Biological Chemistry, University of California, Los Angeles, United States Export Date: 19 January 2022 CODEN: DEVPE Correspondence Address: Banerjee, U.; Department of Molecular, United States; email: banerjee@mbi.ucla.edu AB - Genetic and genomic analysis in Drosophila suggests that hematopoietic progenitors likely transition into terminal fates via intermediate progenitors (IPs) with some characteristics of either, but perhaps maintaining IP-specific markers. In the past, IPs have not been directly visualized and investigated owing to lack of appropriate genetic tools. Here, we report a Split GAL4 construct, CHIZ-GAL4, that identifies IPs as cells physically juxtaposed between true progenitors and differentiating hemocytes. IPs are a distinct cell type with a unique cell-cycle profile and they remain multipotent for all blood cell fates. In addition, through their dynamic control of the Notch ligand Serrate, IPs specify the fate of direct neighbors. The Ras pathway controls the number of IP cells and promotes their transition into differentiating cells. This study suggests that it would be useful to characterize such intermediate populations of cells in mammalian hematopoietic systems. © 2021. Published by The Company of Biologists Ltd LA - English DB - MTMT ER - TY - JOUR AU - Tattikota, Sudhir Gopal AU - Perrimon, Norbert TI - Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing JF - BIO-PROTOCOL J2 - BIO-PROTOCOL VL - 11 PY - 2021 IS - 16 PG - 8 SN - 2331-8325 DO - 10.21769/BioProtoc.4127 UR - https://m2.mtmt.hu/api/publication/32362073 ID - 32362073 N1 - Export Date: 19 January 2022 Correspondence Address: Tattikota, S.G.; Department of Genetics, United States; email: sudhir_gt@hms.harvard.edu Correspondence Address: Perrimon, N.; Department of Genetics, United States; email: perrimon@genetics.med.harvard.edu AB - Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain high-quality single cells in suspension. Recently, we used this approach to characterize Drosophila blood cells (hemocytes). Here, we provide a detailed protocol for obtaining single hemocytes in suspension, which can be used for microfluidics-based scRNA-seq platforms. This protocol involves the simple bleeding of third instar larvae and the subsequent purification of the hemolymph using either Optiprep-based gradient centrifugation or traditional centrifugation methods to obtain single hemocytes of high quality for scRNA-seq. Importantly, this method for single-hemocyte preparation is straightforward and reproducible, with negligible issues associated with cell viability as the entire procedure involves no enzymatic dissociation.[GRAPHICS]. LA - English DB - MTMT ER - TY - JOUR AU - Trainor, J.E. AU - Pooja, K.R. AU - Mortimer, N.T. TI - Immune cell production is targeted by parasitoid wasp virulence in a drosophila–parasitoid wasp interaction JF - PATHOGENS J2 - PATHOGENS VL - 10 PY - 2021 IS - 1 SP - 1 EP - 16 PG - 16 SN - 2076-0817 DO - 10.3390/pathogens10010049 UR - https://m2.mtmt.hu/api/publication/31868552 ID - 31868552 N1 - Export Date: 12 February 2021 Correspondence Address: Mortimer, N.T.; School of Biological Sciences, United States; email: ntmorti@ilstu.edu Export Date: 14 February 2021 Correspondence Address: Mortimer, N.T.; School of Biological Sciences, United States; email: ntmorti@ilstu.edu AB - The interactions between Drosophila melanogaster and the parasitoid wasps that infect Drosophila species provide an important model for understanding host–parasite relationships. Following parasitoid infection, D. melanogaster larvae mount a response in which immune cells (hemocytes) form a capsule around the wasp egg, which then melanizes, leading to death of the parasitoid. Previous studies have found that host hemocyte load; the number of hemocytes available for the encapsulation response; and the production of lamellocytes, an infection induced hemocyte type, are major determinants of host resistance. Parasitoids have evolved various virulence mechanisms to overcome the immune response of the D. melanogaster host, including both active immune suppression by venom proteins and passive immune evasive mechanisms. We identified a previously undescribed parasitoid species, Asobara sp. AsDen, which utilizes an active virulence mechanism to infect D. melanogaster hosts. Asobara sp. AsDen infection inhibits host hemocyte expression of msn, a member of the JNK signaling pathway, which plays a role in lamellocyte production. Asobara sp. AsDen infection restricts the production of lamellocytes as assayed by hemocyte cell morphology and altered msn expression. Our findings suggest that Asobara sp. AsDen infection alters host signaling to suppress immunity. © 2021 by the authors. Li-censee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Wan, Bin AU - Belghazi, Maya AU - Lemauf, Severine AU - Poirie, Marylene AU - Gatti, Jean-Luc TI - Proteomics of purified lamellocytes from Drosophila melanogaster HopTum-l identifies new membrane proteins and networks involved in their functions JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 134 PY - 2021 PG - 21 SN - 0965-1748 DO - 10.1016/j.ibmb.2021.103584 UR - https://m2.mtmt.hu/api/publication/32358968 ID - 32358968 N1 - Université Côte d'Azur, INRAE, CNRS, Institute Sophia-Agrobiotech, Sophia Antipolis, France Institute of NeuroPhysiopathology (INP), UMR7051, CNRS, Aix-Marseille Université, Marseille, 13015, France Cited By :1 Export Date: 19 January 2022 CODEN: IBMBE Correspondence Address: Gatti, J.-L.; Sophia Agrobiotech Institute (ISA), Sophia Antipolis, France; email: jean-luc.gatti@inrae.fr AB - In healthy Drosophila melanogaster larvae, plasmatocytes and crystal cells account for 95% and 5% of the hemocytes, respectively. A third type of hemocytes, lamellocytes, are rare, but their number increases after oviposition by parasitoid wasps. The lamellocytes form successive layers around the parasitoid egg, leading to its encapsulation and melanization, and finally the death of this intruder. However, the total number of lamellocytes per larva remains quite low even after parasitoid infestation, making direct biochemical studies difficult. Here, we used the HopTum-l mutant strain that constitutively produces large numbers of lamellocytes to set up a purification method and analyzed their major proteins by 2D gel electrophoresis and their plasma membrane surface proteins by 1D SDS-PAGE after affinity purification. Mass spectrometry identified 430 proteins from 2D spots and 344 affinity-purified proteins from 1D bands, for a total of 639 unique proteins. Known lamellocyte markers such as PPO3 and the myospheroid integrin were among the components identified with specific chaperone proteins. Affinity purification detected other integrins, as well as a wide range of integrin-associated proteins involved in the formation and function of cell-cell junctions. Overall, the newly identified proteins indicate that these cells are highly adapted to the encapsulation process (recognition, motility, adhesion, signaling), but may also have several other physiological functions (such as secretion and internalization of vesicles) under different signaling pathways. These results provide the basis for further in vivo and in vitro studies of lamellocytes, including the development of new markers to identify coexisting populations and their respective origins and functions in Drosophila immunity. LA - English DB - MTMT ER - TY - JOUR AU - Yang, L. AU - Qiu, L.-M. AU - Fang, Q. AU - Stanley, D.W. AU - Ye, G.-Y. TI - Cellular and humoral immune interactions between Drosophila and its parasitoids JF - INSECT SCIENCE J2 - INSECT SCI VL - 28 PY - 2021 IS - 5 SP - 1208 EP - 1227 PG - 20 SN - 1672-9609 DO - 10.1111/1744-7917.12863 UR - https://m2.mtmt.hu/api/publication/31596046 ID - 31596046 N1 - State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China USDA Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, MO, United States Cited By :28 Export Date: 15 February 2024 Correspondence Address: Ye, G.-Y.; State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, China; email: chu@zju.edu.cn AB - The immune interactions occurring between parasitoids and their host insects, especially in Drosophila–wasp models, have long been the research focus of insect immunology and parasitology. Parasitoid infestation in Drosophila is counteracted by its multiple natural immune defense systems, which include cellular and humoral immunity. Occurring in the hemocoel, cellular immune responses involve the proliferation, differentiation, migration and spreading of host hemocytes and parasitoid encapsulation by them. Contrastingly, humoral immune responses rely more heavily on melanization and on the Toll, Imd and Jak/Stat immune pathways associated with antimicrobial peptides along with stress factors. On the wasps’ side, successful development is achieved by introducing various virulence factors to counteract immune responses of Drosophila. Some or all of these factors manipulate the host's immunity for successful parasitism. Here we review current knowledge of the cellular and humoral immune interactions between Drosophila and its parasitoids, focusing on the defense mechanisms used by Drosophila and the strategies evolved by parasitic wasps to outwit it. © 2020 Institute of Zoology, Chinese Academy of Sciences LA - English DB - MTMT ER - TY - JOUR AU - Al Outa, Amani AU - Abubaker, Dana AU - Madi, Joelle AU - Nasr, Rihab AU - Shirinian, Margret TI - The Leukemic Fly: Promises and Challenges JF - CELLS J2 - CELLS-BASEL VL - 9 PY - 2020 IS - 7 PG - 19 SN - 2073-4409 DO - 10.3390/cells9071737 UR - https://m2.mtmt.hu/api/publication/31468414 ID - 31468414 AB - Leukemia involves different types of blood cancers, which lead to significant mortality and morbidity. Murine models of leukemia have been instrumental in understanding the biology of the disease and identifying therapeutics. However, such models are time consuming and expensive in high throughput genetic and drug screening.Drosophilamelanogasterhas emerged as an invaluable in vivo model for studying different diseases, including cancer. Fruit flies possess several hematopoietic processes and compartments that are in close resemblance to their mammalian counterparts. A number of studies succeeded in characterizing the fly's response upon the expression of human leukemogenic proteins in hematopoietic and non-hematopoietic tissues. Moreover, some of these studies showed that these models are amenable to genetic screening. However, none were reported to be tested for drug screening. In this review, we describe theDrosophilahematopoietic system, briefly focusing on leukemic diseases in which fruit flies have been used. We discuss myeloid and lymphoid leukemia fruit fly models and we further highlight their roles for future therapeutic screening. In conclusion, fruit fly leukemia models constitute an interesting area which could speed up the process of integrating new therapeutics when complemented with mammalian models. LA - English DB - MTMT ER - TY - JOUR AU - Bozler, Julianna AU - Kacsoh, Balint Z. AU - Bosco, Giovanni TI - Maternal Priming of Offspring Immune System in Drosophila JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 10 PY - 2020 IS - 1 SP - 165 EP - 175 PG - 11 SN - 2160-1836 DO - 10.1534/g3.119.400852 UR - https://m2.mtmt.hu/api/publication/31468836 ID - 31468836 N1 - Cited By :7 Export Date: 19 January 2022 Correspondence Address: Bozler, J.; Dartmouth Medical School, Vail 609, 74 College St, United States; email: Lita.Kacsoh@gmail.com AB - Immune priming occurs when a past infection experience leads to a more effective immune response upon a secondary exposure to the infection or pathogen. In some instances, parents are able to transmit immune priming to their offspring, creating a subsequent generation with a superior immune capability, through processes that are not yet fully understood. Using a parasitoid wasp, which infects larval stages of Drosophila melanogaster, we describe an example of an intergenerational inheritance of immune priming. This phenomenon is anticipatory in nature and does not rely on parental infection, but rather, when adult fruit flies are cohabitated with a parasitic wasp, they produce offspring that are more capable of mounting a successful immune response against a parasitic macro-infection. This increase in offspring survival correlates with a more rapid induction of lamellocytes, a specialized immune cell. RNA-sequencing of the female germline identifies several differentially expressed genes following wasp exposure, including the peptiodoglycan recognition protein-LB (PGRP-LB). We find that genetic manipulation of maternal PGRP-LB identifies this gene as a key element in this intergenerational phenotype. LA - English DB - MTMT ER - TY - JOUR AU - Cattenoz, Pierre B. AU - Sakr, Rosy AU - Pavlidaki, Alexia AU - Delaporte, Claude AU - Riba, Andrea AU - Molina, Nacho AU - Hariharan, Nivedita AU - Mukherjee, Tina AU - Giangrande, Angela TI - Temporal specificity and heterogeneity ofDrosophilaimmune cells JF - EMBO JOURNAL J2 - EMBO J VL - 39 PY - 2020 IS - 12 PG - 25 SN - 0261-4189 DO - 10.15252/embj.2020104486 UR - https://m2.mtmt.hu/api/publication/31460653 ID - 31460653 N1 - Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France Centre National de la Recherche Scientifique, UMR7104, Illkirch, France Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France Université de Strasbourg, Illkirch, France Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, India The University of Trans-disciplinary Health Sciences and Technology, Bangalore, India Cited By :31 Export Date: 19 January 2022 CODEN: EMJOD Correspondence Address: Cattenoz, P.B.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut de Génétique et de Biologie Moléculaire et CellulaireFrance; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Centre National de la Recherche Scientifique, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Centre National de la Recherche Scientifique, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Institut National de la Santé et de la Recherche Médicale, France; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Institut National de la Santé et de la Recherche Médicale, France; email: angela@igbmc.fr Correspondence Address: Cattenoz, P.B.; Université de StrasbourgFrance; email: cattenoz@igbmc.fr Correspondence Address: Giangrande, A.; Université de StrasbourgFrance; email: angela@igbmc.fr AB - Immune cells provide defense against non-self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity ofDrosophilaimmune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single-cell RNA sequencing, we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct processes, e.g., proliferation, phagocytosis, metabolic homeostasis, and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation, which triggers the differentiation of a novel hemocyte type, the lamellocyte. This first molecular atlas of hemocytes provides insights and paves the way to study the biology of theDrosophilaimmune cells in physiological and pathological conditions. LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Lerner, Zita AU - Magyar, Lilla Brigitta AU - Soós, Bálint AU - Tóth, Renáta AU - Kristó, Ildikó AU - Vilmos, Péter AU - Juhász, Gábor AU - Kovács, Attila Lajos AU - Hegedűs, Zoltán AU - Sensen, Christoph W. AU - Kurucz, Judit Éva AU - Andó, István TI - Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid Zaprionus indianus JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 12 PY - 2020 IS - 3 SP - 257 EP - 272 PG - 16 SN - 1662-811X DO - 10.1159/000502646 UR - https://m2.mtmt.hu/api/publication/30819399 ID - 30819399 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Grawe, Ferdinand AU - Uhlirova, Mirka TI - Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 PG - 27 SN - 2050-084X DO - 10.7554/eLife.57297 UR - https://m2.mtmt.hu/api/publication/31686151 ID - 31686151 N1 - Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany Molecular Cell Biology, Institute I for Anatomy, University of Cologne Medical School, Cologne, Germany Export Date: 8 March 2021 Correspondence Address: Csordás, G.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: cgabor@uni-koeln.de Correspondence Address: Uhlirova, M.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: mirka.uhlirova@uni-koeln.de Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany Molecular Cell Biology, Institute I for Anatomy, University of Cologne Medical School, Cologne, Germany Cited By :1 Export Date: 10 May 2021 Correspondence Address: Csordás, G.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: cgabor@uni-koeln.de Correspondence Address: Uhlirova, M.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: mirka.uhlirova@uni-koeln.de AB - Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation. The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood. Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments. We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment. We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association. These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila LA - English DB - MTMT ER - TY - JOUR AU - Du, Jie AU - Lin, Zhe AU - Volovych, Olga AU - Lu, Zhiqiang AU - Zou, Zhen TI - A RhoGAP venom protein from Microplitis mediator suppresses the cellular response of its host Helicoverpa armigera JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 108 PY - 2020 PG - 11 SN - 0145-305X DO - 10.1016/j.dci.2020.103675 UR - https://m2.mtmt.hu/api/publication/31468869 ID - 31468869 N1 - State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, 100049, China Department of Entomology, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, 311300, China Cited By :1 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Zou, Z.; State Key Laboratory of Integrated Management of Pest Insects and Rodents, China; email: zouzhen@ioz.ac.cn AB - Female parasitoid wasps normally inject virulence factors together with eggs into their host to counter host immunity defenses. A newly identified RhoGAP protein in the venom of Microplitis mediator compromises the cellular immunity of its host, Helicoverpa armigera. RhoGAP1 proteins entered H. armigera hemocytes, and the host cellular cytoskeleton was disrupted. Depletion of MmGAP1 by injection of dsRNA or antibody increased the wasp egg encapsulation rate. An immunoprecipitation assay of overexpressed MmGAP1 protein in a Helicoverpa cell line showed that MmGAP1 interacts with many cellular cytoskeleton associated proteins as well as Rho GTPases. A yeast two-hybrid and a pull-down assay demonstrated that MmGAP1 interacts with H. armigera RhoA and Cdc42. These results show that the RhoGAP protein in M. mediator can destroy the H. armigera hemocyte cellular cytoskeleton, restrain host cellular immune defense, and increase the probability of successful parasitism. LA - English DB - MTMT ER - TY - JOUR AU - Dziedziech, Alexis AU - Shivankar, Sai AU - Theopold, Ulrich TI - Drosophilamelanogaster Responses against Entomopathogenic Nematodes: Focus on Hemolymph Clots JF - INSECTS J2 - INSECTS VL - 11 PY - 2020 IS - 1 PG - 11 SN - 2075-4450 DO - 10.3390/insects11010062 UR - https://m2.mtmt.hu/api/publication/31470099 ID - 31470099 N1 - Cited By :5 Export Date: 19 January 2022 Correspondence Address: Theopold, U.; Department of Molecular Biosciences, Sweden; email: uli.theopold@su.se AB - Several insect innate immune mechanisms are activated in response to infection by entomopathogenic nematodes (EPNs). In this review, we focus on the coagulation of hemolymph, which acts to stop bleeding after injury and prevent access of pathogens to the body cavity. After providing a general overview of invertebrate coagulation systems, we discuss recent findings in Drosophila melanogaster which demonstrate that clots protect against EPN infections. Detailed analysis at the cellular level provided insight into the kinetics of the secretion of Drosophila coagulation factors, including non-classical modes of secretion. Roughly, clot formation can be divided into a primary phase in which crosslinking of clot components depends on the activity of Drosophila transglutaminase and a secondary, phenoloxidase (PO)-dependent phase, characterized by further hardening and melanization of the clot matrix. These two phases appear to play distinct roles in two commonly used EPN infection models, namely Heterorhabditis bacteriophora and Steinernema carpocapsae. Finally, we discuss the implications of the coevolution between parasites such as EPNs and their hosts for the dynamics of coagulation factor evolution. LA - English DB - MTMT ER - TY - JOUR AU - Ghosh, S. AU - Ghosh, S. AU - Mandal, L. TI - Drosophila metamorphosis involves hemocyte mediated macroendocytosis and efferocytosis JF - INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY J2 - INT J DEV BIOL VL - 64 PY - 2020 IS - 1-3 SP - 309 EP - 319 PG - 11 SN - 0214-6282 DO - 10.1387/ijdb.190215lm UR - https://m2.mtmt.hu/api/publication/31868548 ID - 31868548 N1 - Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge CityPunjab, India Eunice Kennedy Shriver National Institute of Child Health and Human development (NICHD)National Institute of Health (NIH), Bethesda, MD 20892-3758, United States Export Date: 12 February 2021 CODEN: IJDBE Correspondence Address: Mandal, L.; Developmental Genetics Laboratory, Sector 81, P.O. Manauli, India; email: lolitika@iisermohali.ac.in AB - Drosophila hemocytes are majorly associated with immune responses, but they also undertake several non-immune functions that are crucial during various stages of development. The activity and behaviour of hemocytes are least documented during the metamorphic phase of fly development. Here we describe the activity, form and behaviour of the most abundant type of hemocyte in Drosophila melanogaster, the “plasmatocyte,” throughout pupal development. Our study reveals different forms of plasmatocytes laden with varying degrees of histolyzing debris (muscle and fat) which extend beyond the size of the cell itself, highlighting the phagocytic capacity of these plasmatocytes. Interestingly, the engulfment of apoptotic debris by plasmatocytes is an actin-dependent process, and by the end of metamorphosis, clearance is achieved. The uptake of apoptotic debris consisting of muscles and lipids by the plasmatocytes provides us a model that can be employed to dissect out the relevant components of macroendocytosis and lipid-loaded phagocytosis.This understanding, by itself, is crucial for addressing the emerging role of phagocytes in physiology and pathophysiology. © 2020 UPV/EHU Press. LA - English DB - MTMT ER - TY - JOUR AU - Hillyer, J.F. AU - Pass, G. TI - The insect circulatory system: Structure, function, and evolution JF - ANNUAL REVIEW OF ENTOMOLOGY J2 - ANNU REV ENTOMOL VL - 65 PY - 2020 SP - 121 EP - 143 PG - 23 SN - 0066-4170 DO - 10.1146/annurev-ento-011019-025003 UR - https://m2.mtmt.hu/api/publication/31149641 ID - 31149641 AB - Although the insect circulatory system is involved in a multitude of vital physiological processes, it has gone grossly understudied. This review highlights this critical physiological system by detailing the structure and function of the circulatory organs, including the dorsal heart and the accessory pulsatile organs that supply hemolymph to the appendages. It also emphasizes how the circulatory system develops and ages and how, by means of reflex bleeding and functional integration with the immune system, it supports mechanisms for defense against predators and microbial invaders, respectively. Beyond that, this review details evolutionary trends and novelties associated with this system, as well as the ways in which this system also plays critical roles in thermoregulation and tracheal ventilation in high-performance fliers. Finally, this review highlights how novel discoveries could be harnessed for the control of vector-borne diseases and for translational medicine, and it details principal knowledge gaps that necessitate further investigation. Copyright © 2020 by Annual Reviews. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Koranteng, Ferdinand AU - Cha, Nuri AU - Shin, Mingyu AU - Shim, Jiwon TI - The Role of Lozenge in Drosophila Hematopoiesis JF - MOLECULES AND CELLS J2 - MOL CELLS VL - 43 PY - 2020 IS - 2 SP - 114 EP - 120 PG - 7 SN - 1016-8478 DO - 10.14348/molcells.2019.0249 UR - https://m2.mtmt.hu/api/publication/31470092 ID - 31470092 AB - Drosophila hematopoiesis is comparable to mammalian differentiation of myeloid lineages, and therefore, has been a useful model organism in illustrating the molecular and genetic basis for hematopoiesis. Multiple novel regulators and signals have been uncovered using the tools of Drosophila genetics. A Runt domain protein, lozenge, is one of the first players recognized and closely studied in the hematopoietic lineage specification. Here, we explore the role of lozenge in determination of prohemocytes into a special class of hemocyte, namely the crystal cell, and discuss molecules and signals controlling the lozenge function and its implication in immunity and stress response. Given the highly conserved nature of Runt domain in both invertebrates and vertebrates, studies in Drosophila will enlighten our perspectives on Runx-mediated development and pathologies. LA - English DB - MTMT ER - TY - JOUR AU - Lan, Wenwen AU - Liu, Sumin AU - Zhao, Long AU - Su, Ying TI - Regulation ofDrosophilaHematopoiesis in Lymph Gland: From a Developmental Signaling Point of View JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 15 PG - 15 SN - 1661-6596 DO - 10.3390/ijms21155246 UR - https://m2.mtmt.hu/api/publication/31686813 ID - 31686813 N1 - Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003, China College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China Fisheries College, Ocean University of China, Qingdao, 266003, China Cited By :1 Export Date: 19 January 2022 Correspondence Address: Zhao, L.; Institute of Evolution & Marine Biodiversity, China; email: zhaolong@ouc.edu.cn Correspondence Address: Zhao, L.; Fisheries College, China; email: zhaolong@ouc.edu.cn Correspondence Address: Su, Y.; Institute of Evolution & Marine Biodiversity, China; email: suying@ouc.edu.cn Correspondence Address: Su, Y.; College of Marine Life Sciences, China; email: suying@ouc.edu.cn AB - TheDrosophilahematopoietic system is becoming increasingly attractive for its simple blood cell lineage and its developmental and functional parallels with the vertebrate system. As the dedicated organ forDrosophilalarval hematopoiesis, the lymph gland harbors both multipotent stem-like progenitor cells and differentiated blood cells. The balance between progenitor maintenance and differentiation in the lymph gland must be precisely and tightly controlled. Multiple developmental signaling pathways, such as Notch, Hedgehog, and Wnt/Wingless, have been demonstrated to regulate the hematopoietic processes in the lymph gland. Focusing on blood cell maintenance and differentiation, this article summarizes the functions of several classic developmental signaling pathways for lymph gland growth and patterning, highlighting the important roles of developmental signaling during lymph gland development as well asDrosophilalarval hematopoiesis. LA - English DB - MTMT ER - TY - JOUR AU - Lapointe, Jason F. AU - McCarthy, Connor D. AU - Dunphy, Gary B. AU - Mandato, Craig A. TI - Physiological evidence of integrin-antibody reactive proteins influencing the innate cellular immune responses of larval Galleria mellonella hemocytes JF - INSECT SCIENCE J2 - INSECT SCI VL - 27 PY - 2020 IS - 2 SP - 239 EP - 255 PG - 17 SN - 1672-9609 DO - 10.1111/1744-7917.12646 UR - https://m2.mtmt.hu/api/publication/31470100 ID - 31470100 N1 - Department of Anatomy and Cell Biology, McGill University, Montreal, Canada Department of Natural Resource Sciences, Macdonald Campus, Ste. Anne De Bellevue, Canada Cited By :3 Export Date: 19 January 2022 Correspondence Address: Dunphy, G.B.; Department of Natural Resource Sciences, Ste. Anne De Bellevue, Canada; email: gary.dunphy@mcgill.ca AB - Larval Galleria mellonella (L.) hemocytes form microaggregates in response to stimulation by Gram-positive bacteria. Hemocyte adhesion to foreign materials is mediated by the cAMP/ protein kinase A pathway and the beta-subunit of cholera toxin using a cAMP-independent mechanism. Cholera toxin-induced microaggregation was inhibited by the integrin inhibitory RGDS peptide, implying integrins may be part of the mechanism. Based on the types of mammalian integrin-antibody reactive proteins affecting hemocyte adhesion and bacterial-induced responses alpha(5), alpha(v), beta(1), and beta(3) subunits occurred on both granular cell and plasmatocyte hemocyte subtypes. A fluorescent band representing the binding of rabbit alpha(5)-integrin subunit antibodies occurred between adhering heterotypic hemocytes. The frequency of the bands was increased by cholera toxin. The alpha(5) and beta(1) rabbit integrin subunit antibodies inhibited removal of Bacillus subtilis (Cohn) from the hemolymph in vivo. A alpha(5) beta(1)-specific synthetic peptide blocker similarly diminished hemocyte function whereas the alpha(v) beta(3)-specific inhibitory peptide and the corresponding integrin subunit antibodies did not influence nonself hemocyte activities. Western blots revealed several proteins reacting with a given integrin-antibody subtype. Thus integrin-antibody reactive proteins (which may include integrins) with possible alpha(5) and beta(1) epitopes modulate immediate hemocyte function. Confocal microscopy established plasmatocyte adhesion to and rosetting over substrata followed by granular cell microaggregate adhesion to plasmatocytes during early stage nodulation. LA - English DB - MTMT ER - TY - JOUR AU - Li, Miaomiao AU - Meng, Qian AU - Zhang, Huan AU - Ni, Ruoyao AU - Zhou, Guiling AU - Zhao, Yanni AU - Wu, Peipei AU - Shu, Ruihao AU - Qin, Qilian AU - Zhang, Jihong TI - Vegetative development and host immune interaction of Ophiocordyceps sinensis within the hemocoel of the ghost moth larva, Thitarodes xiaojinensis JF - JOURNAL OF INVERTEBRATE PATHOLOGY J2 - J INVERTEBR PATHOL VL - 170 PY - 2020 PG - 10 SN - 0022-2011 DO - 10.1016/j.jip.2020.107331 UR - https://m2.mtmt.hu/api/publication/31470094 ID - 31470094 N1 - State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China Cited By :5 Export Date: 19 January 2022 CODEN: JIVPA Correspondence Address: Qin, Q.; State Key Laboratory of Integrated Management of Pest Insects and Rodents, China; email: qinql@ioz.ac.cn AB - Ophiocordyceps sinensis is an entomopathogenic fungus that infects ghost moth larva, forming the most valuable and rare traditional Chinese medicine, Chinese cordyceps. Our knowledge of the basic morphology and developmental biology of Chinese cordyceps is limited. In this study, morphological and ultrastructural observations of O. sinensis development in the hemocoel of Thitarodes xiaojinensis were obtained by multiple light and electron microscopy techniques, and the host immune reaction activities were determined. Our results indicated that fungal cells in the host hemocoel underwent morphotype transformations from blastospores to prehyphae to hyphae in sequence. The fusiform yeast-like blastospores were the initial cell type present in the host hemocoel and remained for 5 months or more; the encapsulation reaction and phenoloxidase activity of T. xiaojinensis hemolymph were inhibited during this period. When larvae entered the last instar, the blastospores switched to prehyphae and expanded throughout the host tissues, and then hyphae germinated from the prehyphae and mycelia formed, which finally led to host death. Considering the distinct differences between blastospores and hyphae, we identified prehyphae, which play important roles in fungal expansion, hyphae germination, and fusion formation among filaments. Notably, the elongation of prehyphae was strongly presumed to occur through fission but without separation of the two sister cells, in contrast to blastospore budding. During the morphotype transformation, the amount and composition of lipid droplets changed greatly, suggesting their important roles in these events. Overall, we provide a morphological and ultrastructural characterization of O. sinensis vegetative development within the hemocoel of T. xiaojinensis, identify and name the prehypha fungal cell type in entomopathogenic fungi for the first time, and conclude that O. sinensis infection causes sustained immunosuppression in T. xiaojinensis. LA - English DB - MTMT ER - TY - JOUR AU - Madhwal, S. AU - Shin, M. AU - Kapoor, A. AU - Goyal, M. AU - Joshi, M.K. AU - Rehman, P.M.U. AU - Gor, K. AU - Shim, J. AU - Mukherjee, T. TI - Metabolic control of cellular immune-competency by odors in drosophila JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 SP - 1 EP - 93 PG - 93 SN - 2050-084X DO - 10.7554/ELIFE.60376 UR - https://m2.mtmt.hu/api/publication/31868549 ID - 31868549 N1 - Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, Bangalore, 560065, India Department of Life Science, College of Natural Science, Hanyang University, Seoul, 04763, South Korea Vellore Institute of Technology, Katpadi Road, Vellore, Tamil Nadu 632014, India Research Institute for Natural Science, Hanyang University, Seoul, 04763, South Korea Manipal Academy of Higher Education, Manipal, Karnataka 576104, India The University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka 560064, India Export Date: 12 February 2021 Correspondence Address: Shim, J.; Department of Life Science, South Korea; email: jshim@hanyang.ac.kr Correspondence Address: Mukherjee, T.; Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, India; email: tinam@instem.res.in Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, Bangalore, 560065, India Department of Life Science, College of Natural Science, Hanyang University, Seoul, 04763, South Korea Vellore Institute of Technology, Katpadi Road, Vellore, Tamil Nadu 632014, India Research Institute for Natural Science, Hanyang University, Seoul, 04763, South Korea Manipal Academy of Higher Education, Manipal, Karnataka 576104, India The University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka 560064, India Export Date: 14 February 2021 Correspondence Address: Shim, J.; Department of Life Science, South Korea; email: jshim@hanyang.ac.kr Correspondence Address: Mukherjee, T.; Institute for Stem Cell Science and Regenerative Medicine (inStem), Bellary Road, India; email: tinam@instem.res.in LA - English DB - MTMT ER - TY - JOUR AU - Powers, Joseph C. AU - Turangan, Raymar AU - Joosse, Bryan A. AU - Hillyer, Julian F. TI - Adult Mosquitoes Infected with Bacteria Early in Life Have Stronger Antimicrobial Responses and More Hemocytes after Reinfection Later in Life JF - INSECTS J2 - INSECTS VL - 11 PY - 2020 IS - 6 PG - 15 SN - 2075-4450 DO - 10.3390/insects11060331 UR - https://m2.mtmt.hu/api/publication/31470095 ID - 31470095 N1 - Cited By :4 Export Date: 19 January 2022 Correspondence Address: Hillyer, J.F.; Department of Biological Sciences, United States; email: julian.hillyer@vanderbilt.edu AB - The immunological strategies employed by insects to overcome infection vary with the type of infection and may change with experience. We investigated how a bacterial infection in the hemocoel of the African malaria mosquito,Anopheles gambiae, prepares the immune system to face a subsequent bacterial infection. For this, adult female mosquitoes were separated into three groups-unmanipulated, injured, or infected withEscherichia coli-and five days later all the mosquitoes were infected with a different strain ofE. coli. We found that an injury or a bacterial infection early in life enhances the ability of mosquitoes to kill bacteria later in life. This protection results in higher mosquito survival and is associated with an increased hemocyte density, altered phagocytic activity by individual hemocytes, and the increased expression of nitric oxide synthase and perhaps prophenoloxidase 6. Protection from a second infection likely occurs because of heightened immune awareness due to an already existing infection instead of memory arising from an earlier, cured infection. This study highlights the dynamic nature of the mosquito immune response and how one infection prepares mosquitoes to survive a subsequent infection. LA - English DB - MTMT ER - TY - JOUR AU - Ramond, Elodie AU - Dudzic, Jan Paul AU - Lemaitre, Bruno TI - Comparative RNA-Seq analyses ofDrosophilaplasmatocytes reveal gene specific signatures in response to clean injury and septic injury JF - PLOS ONE J2 - PLOS ONE VL - 15 PY - 2020 IS - 6 PG - 27 SN - 1932-6203 DO - 10.1371/journal.pone.0235294 UR - https://m2.mtmt.hu/api/publication/31468577 ID - 31468577 N1 - Cited By :9 Export Date: 19 January 2022 CODEN: POLNC Correspondence Address: Ramond, E.; Global Health Institute, Switzerland; email: elodie.ramond@inserm.fr AB - Drosophila melanogaster's blood cells (hemocytes) play essential roles in wound healing and are involved in clearing microbial infections. Here, we report the transcriptional changes of larval plasmatocytes after clean injury or infection with the Gram-negative bacteriumEscherichia colior the Gram-positive bacteriumStaphylococcus aureuscompared to hemocytes recovered from unchallenged larvae via RNA-Sequencing. This study reveals 676 differentially expressed genes (DEGs) in hemocytes from clean injury samples compared to unchallenged samples, and 235 and 184 DEGs inE.coliandS.aureussamples respectively compared to clean injury samples. The clean injury samples showed enriched DEGs for immunity, clotting, cytoskeleton, cell migration, hemocyte differentiation, and indicated a metabolic reprogramming to aerobic glycolysis, a well-defined metabolic adaptation observed in mammalian macrophages. Microbial infections trigger significant transcription of immune genes, with significant differences between theE.coliandS.aureussamples suggesting that hemocytes have the ability to engage various programs upon infection. Collectively, our data bring new insights onDrosophilahemocyte function and open the route to post-genomic functional analysis of the cellular immune response. LA - English DB - MTMT ER - TY - BOOK AU - Röszer, Tamás TI - The M2 Macrophage T3 - Progress in Inflammation Research Series, ISSN 1422-7746 ; 86. PB - Springer Netherlands CY - Cham PY - 2020 SP - 400 SP - 224 SN - 9783030504809 DO - 10.1007/978-3-030-50480-9 UR - https://m2.mtmt.hu/api/publication/32661451 ID - 32661451 AB - Macrophages are core components of the innate immune system. Once activated, they may have either pro- or anti-inflammatory effects that include pathogen killing, safe disposal of apoptotic cells or tissue renewal. The activation state of macrophages is conceptualized by the so-called M1/M2 model of polarization. M2 macrophages are not simply antagonists of M1 macrophages; rather, they represent a network of tissue resident macrophages with roles in tissue development and organ homeostasis. M2 macrophages govern functions at the interfaces of immunity, tissue development and turnover, metabolism, and endocrine signaling. Dysfunction in M2 macrophages can ruin the healthy interplay between the immune system and metabolic processes, and lead to diseases such as insulin resistance, metabolic syndrome, and type 1 and 2 diabetes mellitus. Furthermore, M2 macrophages are essential for healthy tissue development and immunological self-tolerance. Worryingly, these functions of M2 macrophages can also be disrupted, resulting in tumor growth and autoimmunity. This book comprehensively discusses the biology of M2 macrophages, summarizes the current state of knowledge, and highlights key questions that remain unanswered. LA - English DB - MTMT ER - TY - JOUR AU - Shin, Mingyu AU - Cha, Nuri AU - Koranteng, Ferdinand AU - Cho, Bumsik AU - Shim, Jiwon TI - Subpopulation of Macrophage-Like Plasmatocytes Attenuates Systemic Growth via JAK/STAT in the Drosophila Fat Body JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 11 PY - 2020 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2020.00063 UR - https://m2.mtmt.hu/api/publication/31467726 ID - 31467726 N1 - Department of Life Science, College of Natural Science, Hanyang University, Seoul, South Korea Research Institute for Natural Science, College of Natural Science, Hanyang University, Seoul, South Korea Research Institute for Convergence of Basic Sciences, College of Natural Science, Hanyang University, Seoul, South Korea Cited By :6 Export Date: 19 January 2022 Correspondence Address: Shim, J.; Department of Life Science, South Korea; email: jshim@hanyang.ac.kr AB - Drosophila hemocytes, like those of mammals, are given rise from two distinctive phases during both the embryonic and larval hematopoiesis. Embryonically derived hemocytes, mostly composed of macrophage-like plasmatocytes, are largely identified by genetic markers. However, the cellular diversity and distinct functions of possible subpopulations within plasmatocytes have not been explored in Drosophila larvae. Here, we show that larval plasmatocytes exhibit differential expressions of Hemolectin (Hml) and Peroxidasin (Pxn) during development. Moreover, removal of plasmatocytes by overexpressing pro-apoptotic genes, hid and reaper in Hml-positive plasmatocytes, feeding high sucrose diet, or wasp infestation results in increased circulating hemocytes that are Hml-negative. Interestingly these Hml-negative plasmatocytes retain Pxn expression, and animals expressing Hml-negative and Pxn-positive subtype largely attenuate growth and abrogate metabolism. Furthermore, elevated levels of a cytokine, unpaired 3, are detected when Hml-positive hemocytes are ablated, which in turn activates JAK/STAT activity in several tissues including the fat body. Finally, we observed that insulin signaling is inhibited in this background, which can be recovered by concurrent loss of upd3. Overall, this study highlights heterogeneity in Drosophila plasmatocytes and a functional plasticity of each subtype, which reaffirms extension of their role beyond immunity into metabolic regulation for cooperatively maintaining internal homeostatic balance. LA - English DB - MTMT ER - TY - JOUR AU - Tattikota, Sudhir Gopal AU - Cho, Bumsik AU - Liu, Yifang AU - Hu, Yanhui AU - Barrera, Victor AU - Steinbaugh, Michael J. AU - Yoon, Sang-Ho AU - Comjean, Aram AU - Li, Fangge AU - Dervis, Franz AU - Hung, Ruei-Jiun AU - Nam, Jin-Wu AU - Sui, Shannan Ho AU - Shim, Jiwon AU - Perrimon, Norbert TI - A single-cell survey of Drosophila blood JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 PG - 35 SN - 2050-084X DO - 10.7554/eLife.54818 UR - https://m2.mtmt.hu/api/publication/31468416 ID - 31468416 N1 - Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States Department of Life Science, Hanyang University, Seoul, South Korea Harvard TH Chan Bioinformatics Core, Boston, United States Howard Hughes Medical Institute, Boston, United States Cited By :37 Export Date: 19 January 2022 Correspondence Address: Tattikota, S.G.; Department of Genetics, United States; email: sudhir_gt@hms.harvard.edu Correspondence Address: Perrimon, N.; Department of Genetics, United States; email: perrimon@receptor.med.harvard.edu Correspondence Address: Perrimon, N.; Howard Hughes Medical InstituteUnited States; email: perrimon@receptor.med.harvard.edu AB - Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions. LA - English DB - MTMT ER - TY - JOUR AU - von Bredow, Yvette M. AU - von Bredow, Christoph-Ruediger AU - Trenczek, Tina E. TI - A novel site of haematopoiesis and appearance and dispersal of distinct haemocyte types in the Manduca sexta embryo (Insecta, Lepidoptera) JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 111 PY - 2020 PG - 20 SN - 0145-305X DO - 10.1016/j.dci.2020.103722 UR - https://m2.mtmt.hu/api/publication/31470097 ID - 31470097 N1 - Justus–Liebig–Universität Gießen, Institut für Allgemeine Zoologie und Entwicklungsbiologie, Zelluläre Erkennungs– und Abwehrprozesse, Stephanstraße 24, Gießen35390, Germany Technische Universität Dresden, Fakultät Biologie, Institut für Zoologie, Professur für Allgemeine Zoologie und Entwicklungsbiologie, Zellescher Web 20 b, Dresden, 01217, Germany Cited By :2 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: von Bredow, Y.M.; Justus–Liebig–Universität Gießen, Stephanstraße 24, Gießen, Germany; email: Yvette.von.Bredow@mail.de AB - With a set of haemocyte specific markers novel findings on haematopoiesis in the Manduca sexta embryo are presented. We identify a hitherto unknown paired haematopoietic cluster, the abdominal haemocyte cluster in abdominal segment 7 (A7-HCC). These clusters are localised at distinct positions and are established at around katatrepsis. Later in embryogenesis, the A7-HCCs disintegrate, thereby releasing numerous embryonic plasmatocytes which disperse both anteriorly and posteriorly. These cells follow stereotypic migration routes projecting anteriorly. The thoracic larval haematopoietic organs are established at around midembryogenesis. We identify embryonic oenocytoids in the M. sexta embryo for the first time. They appear in the head region roughly at the same time as the A7-HCCs occur and successively disperse in the body cavity during development. Localisation of the prophenoloxidase (proPO) mRNA and of the proPO protein are identical. Morphological, cytometric and antigenic traits show three independently generated haemocyte types during embryogenesis. LA - English DB - MTMT ER - TY - JOUR AU - Bailetti, Alessandro A. AU - Negron-Pineiro, Lenny J. AU - Dhruva, Vishal AU - Harsh, Sneh AU - Lu, Sean AU - Bosula, Aisha AU - Bach, Erika A. TI - Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity JF - DISEASE MODELS & MECHANISMS J2 - DIS MODEL MECH VL - 12 PY - 2019 IS - 5 PG - 15 SN - 1754-8403 DO - 10.1242/dmm.038679 UR - https://m2.mtmt.hu/api/publication/30901165 ID - 30901165 N1 - Department of Biochemistry and Molecular Pharmacology, New York University, School of Medicine, New York, NY 10016, United States Helen L. and Martin S. Kimmel Center for Stem Cell Biology, New York University, School of Medicine, New York, NY 10016, United States Program in Epithelial Biology, Department of Dermatology, Stanford University, School of Medicine, Stanford, CA 94305, United States Cited By :5 Export Date: 19 January 2022 Correspondence Address: Bach, E.A.; Department of Biochemistry and Molecular Pharmacology, United States; email: erika.bach@nyu.edu AB - Myeloproliferative neoplasms (MPNs) are clonal hematopoietic disorders that cause excessive production of myeloid cells. Most MPN patients have a point mutation in JAK2 (JAK2(V617F)), which encodes a dominant-active kinase that constitutively triggers JAK/STAT signaling. In Drosophila, this pathway is simplified, with a singleJAK, Hopscotch (Hop), and a single STAT transcription factor, Stat92E. The hop(Tumorous-lethal) [hop(Tum)] allele encodes a dominant-active kinase that induces sustained Stat92E activation. Like MPN patients, hop(Tum) mutants have significantly more myeloid cells, which form invasive tumors. Through an unbiased genetic screen, we found that heterozygosity for Enhancer of Polycomb [E(Pc)], a component of the Tip60 lysine acetyltransferase complex (also known as KAT5 in humans), significantly increased tumor burden in hopTum animals. Hematopoietic depletion of E(Pc) or other Tip60 components in an otherwise wild-type background also induced blood cell tumors. The E(Pc) tumor phenotype was dependent on JAK/STAT activity, as concomitant depletion of hop or Stat92E inhibited tumor formation. Stat92E target genes were significantly upregulated in E(Pc)-mutant myeloid cells, indicating that loss of E(Pc) activates JAK STAT signaling. Neither the hop nor Stat92E gene was upregulated upon hematopoietic E(Pc) depletion, suggesting that the regulation of the JAK STAT pathway by E(Pc) is dependent on substrates other than histones. Indeed, E(Pc) depletion significantly increased expression of Hop protein in myeloid cells. This study indicates that E(Pc) works as a tumor suppressor by attenuating Hop protein expression and ultimately JAK STAT signaling. Since loss-of-function mutations in the human homologs of E(Pc) and Tip60 are frequently observed in cancer, our work could lead to new treatments for MPN patients.This article has an associated First Person interview with the first author of the paper. LA - English DB - MTMT ER - TY - JOUR AU - Banerjee, Utpal AU - Girard, Juliet R. AU - Goins, Lauren M. AU - Spratford, Carrie M. TI - Drosophila as a Genetic Model for Hematopoiesis JF - GENETICS J2 - GENETICS VL - 211 PY - 2019 IS - 2 SP - 367 EP - 417 PG - 51 SN - 0016-6731 DO - 10.1534/genetics.118.300223 UR - https://m2.mtmt.hu/api/publication/30510237 ID - 30510237 N1 - Export Date: 13 May 2019 CODEN: GENTA Correspondence Address: Banerjee, U.; University of California, 610 Charles E Young Drive East, United States; email: banerjee@mbi.ucla.edu AB - In this FlyBook chapter, we present a survey of the current literature on the development of the hematopoietic system in Drosophila. The Drosophila blood system consists entirely of cells that function in innate immunity, tissue integrity, wound healing, and various forms of stress response, and are therefore functionally similar to myeloid cells in mammals. The primary cell types are specialized for phagocytic, melanization, and encapsulation functions. As in mammalian systems, multiple sites of hematopoiesis are evident in Drosophila and the mechanisms involved in this process employ many of the same molecular strategies that exemplify blood development in humans. Drosophila blood progenitors respond to internal and external stress by coopting developmental pathways that involve both local and systemic signals. An important goal of these Drosophila studies is to develop the tools and mechanisms critical to further our understanding of human hematopoiesis during homeostasis and dysfunction. LA - English DB - MTMT ER - TY - JOUR AU - Bosch, Pablo Sanchez AU - Makhijani, Kalpana AU - Herboso, Leire AU - Gold, Katrina S. AU - Baginsky, Rowan AU - Woodcock, Katie J. AU - Alexander, Brandy AU - Kukar, Katelyn AU - Corcoran, Sean AU - Jacobs, Thea AU - Ouyang, Debra AU - Wong, Corinna AU - Ramond, Elodie J. V. AU - Rhiner, Christa AU - Moreno, Eduardo AU - Lemaitre, Bruno AU - Geissmann, Frederic AU - Bruckner, Katja TI - Adult Drosophila Lack Hematopoiesis but Rely on a Blood Cell Reservoir at the Respiratory Epithelia to Relay Infection Signals to Surrounding Tissues JF - DEVELOPMENTAL CELL J2 - DEV CELL VL - 51 PY - 2019 IS - 6 SP - 787 EP - + PG - 22 SN - 1534-5807 DO - 10.1016/j.devcel.2019.10.017 UR - https://m2.mtmt.hu/api/publication/31072775 ID - 31072775 N1 - Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, United States Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, United States Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, United States King's College London, London, United Kingdom Memorial Sloan Kettering Cancer Center, New York, NY, United States EPFL, Lausanne, Switzerland Champalimaud Center for the Unknown, Lisbon, Portugal Cited By :1 Export Date: 19 February 2020 CODEN: DCEEB Correspondence Address: Brückner, K.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San FranciscoUnited States; email: katja.brueckner@ucsf.edu Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, United States Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, United States Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, United States King's College London, London, United Kingdom Memorial Sloan Kettering Cancer Center, New York, NY, United States EPFL, Lausanne, Switzerland Champalimaud Center for the Unknown, Lisbon, Portugal Cited By :6 Export Date: 12 February 2021 CODEN: DCEEB Correspondence Address: Brückner, K.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, United States; email: katja.brueckner@ucsf.edu AB - The use of adult Drosophila melanogaster as a model for hematopoiesis or organismal immunity has been debated. Addressing this question, we identify an extensive reservoir of blood cells (hemocytes) at the respiratory epithelia (tracheal air sacs) of the thorax and head. Lineage tracing and functional analyses demonstrate that the majority of adult hemocytes are phagocytic macrophages (plasmatocytes) from the embryonic lineage that parallels vertebrate tissue macrophages. Surprisingly, we find no sign of adult hemocyte expansion. Instead, hemocytes play a role in relaying an innate immune response to the blood cell reservoir: through Mid signaling and the Jak/Stat pathway ligand Upd3, hemocytes act as sentinels of bacterial infection, inducing expression of the antimicrobial peptide Drosocin in respiratory epithelia and colocalizing fat body domains. Drosocin expression in turn promotes animal survival after infection. Our work identifies a multisignal relay of organismal humoral immunity, establishing adult Drosophila as model for inter-organ immunity. LA - English DB - MTMT ER - TY - JOUR AU - Cevik, Duygu AU - Acker, Meryl AU - Michalski, Camilla AU - Jacobs, J. Roger TI - Pericardin, a Drosophila collagen, facilitates accumulation of hemocytes at the heart JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 454 PY - 2019 IS - 1 SP - 52 EP - 65 PG - 14 SN - 0012-1606 DO - 10.1016/j.ydbio.2019.06.006 UR - https://m2.mtmt.hu/api/publication/30907377 ID - 30907377 N1 - Cited By :10 Export Date: 19 January 2022 CODEN: DEBIA Correspondence Address: Jacobs, J.R.; Department of Biology, 1280 Main St. West, Canada; email: jacobsr@mcmaster.ca AB - Hematopoietic cell lineages support organismal needs by responding to positional and systemic signals that balance proliferative and differentiation events. Drosophila provides an excellent genetic model to dissect these signals, where the activity of cues in the hemolymph or substrate can be traced to determination and differentiation events of well characterized hemocyte types. Plasmatocytes in third instar larvae increase in number in response to infection and in anticipation of metamorphosis. Here we characterize hemocyte clustering, proliferation and transdifferentiation on the heart or dorsal vessel. Hemocytes accumulate on the inner foldings of the heart basement membrane, where they move with heart contraction, and are in proximity to the heart ostia and pericardial nephrocytes. The numbers of hemocytes vary, but increase transiently before pupariation, and decrease by 4 h before pupa formation. During their accumulation at the heart, plasmatocytes can proliferate and can transdifferentiate into crystal cells. Serrate expressing cells as well as lamellocyte-like, Atilla expressing ensheathing cells are associated with some, but not all hemocyte clusters. Hemocyte aggregation is enhanced by the presence of a heart specific Collagen, Pericardin, but not the associated pericardial cells. The varied and transient number of hemocytes in the pericardial compartment suggests that this is not a hematopoietic hub, but a niche supporting differentiation and rapid dispersal in response to systemic signals. LA - English DB - MTMT ER - TY - JOUR AU - Kim-Jo, Chami AU - Gatti, Jean-Luc AU - Poirie, Marylene TI - Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process JF - FRONTIERS IN PHYSIOLOGY J2 - FRONT PHYSIOL VL - 10 PY - 2019 PG - 8 SN - 1664-042X DO - 10.3389/fphys.2019.00603 UR - https://m2.mtmt.hu/api/publication/30907275 ID - 30907275 N1 - Cited By :19 Export Date: 19 January 2022 Correspondence Address: Poirié, M.; INRA, France; email: marylene.poirie@univ-cotedazur.fr AB - Host-parasitoid interactions are among the most studied interactions between invertebrates because of their fundamental interest - the evolution of original traits in parasitoids - and applied, parasitoids being widely used in biological control. Immunity, and in particular cellular immunity, is central in these interactions, the host encapsulation response being specific for large foreign bodies such as parasitoid eggs. Although already well studied in this species, recent data on Drosophila melanogaster have unquestionably improved knowledge of invertebrate cellular immunity. At the same time, the venomics of parasitoids has expanded, notably those of Drosophila. Here, we summarize and discuss these advances, with a focus on an emerging "time-dependent" view of interactions outcome at the intra- and interspecific level. We also present issues still in debate and prospects for study. Data on the Drosophila-parasitoid model paves the way to new concepts in insect immunity as well as parasitoid wasp strategies to overcome it. LA - English DB - MTMT ER - TY - JOUR AU - Li, Fang AU - Xu, Limei AU - Hui, Xuan AU - Huang, Wanzhen AU - Yang, Feng TI - Directed differentiation of granular cells from crayfish hematopoietic tissue cells JF - FISH AND SHELLFISH IMMUNOLOGY J2 - FISH SHELLFISH IMMUN VL - 88 PY - 2019 SP - 28 EP - 35 PG - 8 SN - 1050-4648 DO - 10.1016/j.fsi.2019.02.054 UR - https://m2.mtmt.hu/api/publication/30912668 ID - 30912668 N1 - Key Laboratory of Marine Genetic Resources, State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China Fujian Key Laboratory of Marine Genetic Resources, Xiamen, 361005, China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Cited By :2 Export Date: 19 January 2022 CODEN: FSIME Correspondence Address: Li, F.; Third Institute of Oceanography, 184# Daxue Road, China; email: lifang@tio.org.cn AB - Hemocytes are the major immune cells of crustaceans. New hemocyte production is required throughout the life cycle of these animals to maintain a functional immune system. The mechanism of crustacean hematopoiesis has just begun to be understood and new methods are needed for the investigation of this process. Here we report the directed differentiation of granular cells (GCs) from the hematopoietic tissue (HPT) cells of Cherax quadricarinatus in vitro. We started by providing the cultured HPT cells with different additives to induce possible differentiation. We found that crayfish muscle extract greatly promoted the physical status of the cells and induced the formation of refractile cytoplasmic granules. The transcription of marker genes and the production of functional prophenoloxidase further confirmed the formation of mature GCs. In our experiments, young GCs usually started to develop in 2 weeks post induction and over 60% of the cells became mature within 3-4 weeks. This is the first time that the fully differentiation of crustacean hemocytes is accomplished in vitro. It provides a powerful tool for in-depth study of crustacean hematopoiesis. LA - English DB - MTMT ER - TY - JOUR AU - Melcarne, Claudia AU - Ramond, Elodie AU - Dudzic, Jan AU - Bretscher, Andrew AU - Kurucz, Judit Éva AU - Andó, István AU - Lemaitre, Bruno TI - Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster JF - FEBS JOURNAL J2 - FEBS J VL - 286 PY - 2019 IS - 14 SP - 2670 EP - 2691 PG - 22 SN - 1742-464X DO - 10.1111/febs.14857 UR - https://m2.mtmt.hu/api/publication/30641961 ID - 30641961 N1 - Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary Cited By :13 Export Date: 19 January 2022 CODEN: FJEOA Correspondence Address: Melcarne, C.; Global Health Institute, Switzerland; email: claudia.melcarne@epfl.ch LA - English DB - MTMT ER - TY - JOUR AU - Paddibhatla, I. AU - Gautam, D.K. AU - Mishra, R.K. TI - SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 456 PY - 2019 IS - 1 SP - 74 EP - 85 PG - 12 SN - 0012-1606 DO - 10.1016/j.ydbio.2019.08.008 UR - https://m2.mtmt.hu/api/publication/31149702 ID - 31149702 N1 - Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad, Telangana state 500007, India Stem Cells and Haematological Disorders Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana state 500046, India Export Date: 31 January 2020 CODEN: DEBIA Correspondence Address: Paddibhatla, I.; Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, India; email: paddibhatla@gmail.com Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad, Telangana state 500007, India Stem Cells and Haematological Disorders Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana state 500046, India Export Date: 3 February 2020 CODEN: DEBIA Correspondence Address: Paddibhatla, I.; Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, India; email: paddibhatla@gmail.com Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad, Telangana state 500007, India Stem Cells and Haematological Disorders Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana state 500046, India Export Date: 19 February 2020 CODEN: DEBIA Correspondence Address: Paddibhatla, I.; Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, India; email: paddibhatla@gmail.com AB - Proper genetic and epigenetic regulation is necessary to maintain the identity and integrity of cells. Enzymes involved in post-transcriptional modifications of histones are key factors in epigenetic mechanisms. Such modifications are also gaining importance for their role in growth and development of cancer. SETDB1 catalyzes the epigenetic mark of lysine-9 methylation of histone-3. In this study, we explored the role of SETDB1 in Drosophila hematopoiesis. We show that SETDB1 controls the differentiation of matured blood cells in wandering third instar larvae. There are three matured blood cells in wild type Drosophila melanogaster: plasmatocytes, crystal cells and lamellocytes. We found that loss-of-function mutants of SETDB1 show hematopoietic defects; increased blood cell proliferation, decreased number of crystal cells, greater differentiation of blood cells into lamellocytes, dysplasia of the anterior lobes of lymph gland and presence of hematopoietic tumors. Cell type specific knockdown of SETDB1 provided similar phenotype i.e., decreased number of crystal cells and an increase in lamellocyte differentiation. In animals with loss of function of SETDB1, Notch pathway was downregulated. Further, over-expression of SETDB1 in blood cells resulted in an increase in the number of crystal cells. This increase is accompanied with an increase in the number of NotchICD expressing cells. We therefore performed genetic rescue using UAS-GAL4 system to rescue loss of function SETDB1 mutants. Our data show that the rescued larvae carrying a wild type copy of SETDB1 in mutant background are devoid of blood tumors. We have identified a novel dual function of SETDB1 methylatransferase as a critical regulator of two of the matured hemocytes, crystal cells and lamellocytes. We propose a novel role of SETDB1 in modulating the differentiation of crystal cells and lamellocytes from a common progenitor and underscore the importance of SETDB1 in Drosophila blood tumor suppression. © 2019 The Authors LA - English DB - MTMT ER - TY - JOUR AU - Valanne, Susanna AU - Salminen, Tiina S. AU - Jarvela-Stolting, Mirva AU - Vesala, Laura AU - Ramet, Mika TI - Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 15 PY - 2019 IS - 1 PG - 28 SN - 1553-7366 DO - 10.1371/journal.ppat.1007504 UR - https://m2.mtmt.hu/api/publication/30509856 ID - 30509856 N1 - Laboratory of Experimental Immunology, BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland PEDEGO Research Unit, and Medical Research Center Oulu, University of Oulu, Department of Children and Adolescents, Oulu University Hospital, Oulu, Finland Department of Pediatrics, Tampere University Hospital, Tampere, Finland Cited By :18 Export Date: 19 January 2022 Correspondence Address: Rämet, M.; Laboratory of Experimental Immunology, Finland; email: mika.ramet@uta.fi AB - Non-coding RNAs have important roles in regulating physiology, including immunity. Here, we performed transcriptome profiling of immune-responsive genes in Drosophila melanogaster during a Gram-positive bacterial infection, concentrating on long non-coding RNA (lncRNA) genes. The gene most highly induced by a Micrococcus luteus infection was CR44404, named Induced by Infection (lincRNA-IBIN). lincRNA-IBIN is induced by both Gram-positive and Gram-negative bacteria in Drosophila adults and parasitoid wasp Leptopilina boulardi in Drosophila larvae, as well as by the activation of the Toll or the Imd pathway in unchallenged flies. We show that upon infection, lincRNA-IBIN is expressed in the fat body, in hemocytes and in the gut, and its expression is regulated by NF-B signaling and the chromatin modeling brahma complex. In the fat body, overexpression of lincRNA-IBIN affected the expression of Toll pathway -mediated genes. Notably, overexpression of lincRNA-IBIN in unchallenged flies elevated sugar levels in the hemolymph by enhancing the expression of genes important for glucose retrieval. These data show that lncRNA genes play a role in Drosophila immunity and indicate that lincRNA-IBIN acts as a link between innate immune responses and metabolism. LA - English DB - MTMT ER - TY - JOUR AU - Varga, Gergely István AU - Csordás, Gábor AU - Cinege, Gyöngyi Ilona AU - Jankovics, Ferenc AU - Sinka, Rita AU - Kurucz, Judit Éva AU - Andó, István AU - Honti, Viktor TI - Headcase is a Repressor of Lamellocyte Fate in Drosophila melanogaster JF - GENES J2 - GENES-BASEL VL - 10 PY - 2019 IS - 3 PG - 17 SN - 2073-4425 DO - 10.3390/genes10030173 UR - https://m2.mtmt.hu/api/publication/30585796 ID - 30585796 N1 - Laboratory of Immunology, Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Laboratory of Drosophila Germ Cell Differentiation, Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Department of Genetics, Faculty of Science and Informatics, University of Szeged, Szeged, 6726, Hungary Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, 50931, Germany Cited By :3 Export Date: 19 January 2022 Correspondence Address: Andó, I.; Laboratory of Immunology, Hungary; email: ando.istvan@brc.mta.hu AB - Due to the evolutionary conservation of the regulation of hematopoiesis, Drosophila provides an excellent model organism to study blood cell differentiation and hematopoietic stem cell (HSC) maintenance. The larvae of Drosophila melanogaster respond to immune induction with the production of special effector blood cells, the lamellocytes, which encapsulate and subsequently kill the invader. Lamellocytes differentiate as a result of a concerted action of all three hematopoietic compartments of the larva: the lymph gland, the circulating hemocytes, and the sessile tissue. Within the lymph gland, the communication of the functional zones, the maintenance of HSC fate, and the differentiation of effector blood cells are regulated by a complex network of signaling pathways. Applying gene conversion, mutational analysis, and a candidate based genetic interaction screen, we investigated the role of Headcase (Hdc), the homolog of the tumor suppressor HECA in the hematopoiesis of Drosophila. We found that naive loss-of-function hdc mutant larvae produce lamellocytes, showing that Hdc has a repressive role in effector blood cell differentiation. We demonstrate that hdc genetically interacts with the Hedgehog and the Decapentaplegic pathways in the hematopoietic niche of the lymph gland. By adding further details to the model of blood cell fate regulation in the lymph gland of the larva, our findings contribute to the better understanding of HSC maintenance. LA - English DB - MTMT ER - TY - JOUR AU - Wan, Bin AU - Goguet, Emilie AU - Ravallec, Marc AU - Pierre, Olivier AU - Lemauf, Severine AU - Volkoff, Anne-Nathalie AU - Gatti, Jean-Luc AU - Poirie, Marylone TI - Venom Atypical Extracellular Vesicles as Interspecies Vehicles of Virulence Factors Involved in Host Specificity: The Case of a Drosophila Parasitoid Wasp JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 10 PY - 2019 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2019.01688 UR - https://m2.mtmt.hu/api/publication/30907274 ID - 30907274 N1 - Université Côte d'Azur, CNRS, ISA, Sophia Antipolis, France Univ. Montpellier, UMR 1333 'Microorganism and Insect Diversity, Genomes and Interactions' (DGIMI), Montpellier, France State Key Laboratory of Rice Biology and Ministry of Agricultural and Rural Affairs, Institute of Insect Sciences, Zhejiang University, Hangzhou, China Henry M. Jackson Foundation at the Uniformed Services University of the Health Sciences, Department of Microbiology and Immunology, Bethesda, MD, United States Cited By :19 Export Date: 19 January 2022 Correspondence Address: Poirié, M.; Université Côte d'Azur, France; email: marylene.poirie@univ-cotedazur.fr AB - Endoparasitoid wasps, which lay eggs inside the bodies of other insects, use various strategies to protect their offspring from the host immune response. The hymenopteran species of the genus Leptopilina, parasites of Drosophila, rely on the injection of a venom which contains proteins and peculiar vesicles (hereafter venosomes). We show here that the injection of purified L. boulardi venosomes is sufficient to impair the function of the Drosophila melanogaster lamellocytes, a hemocyte type specialized in the defense against wasp eggs, and thus the parasitic success of the wasp. These venosomes seem to have a unique extracellular biogenesis in the wasp venom apparatus where they acquire specific secreted proteins/virulence factors and act as a transport system to deliver these compounds into host lamellocytes. The level of venosomes entry into lamellocytes of different Drosophila species was correlated with the rate of parasitism success of the wasp, suggesting that this venosome-cell interaction may represent a new evolutionary level of host-parasitoid specificity. LA - English DB - MTMT ER - TY - JOUR AU - Baldeosingh, Rajkumar AU - Gao, Hongjuan AU - Wu, Xiaorong AU - Fossett, Nancy TI - Hedgehog signaling from the Posterior Signaling Center maintains U-shaped expression and a prohemocyte population in Drosophila JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 441 PY - 2018 IS - 1 SP - 132 EP - 145 PG - 14 SN - 0012-1606 DO - 10.1016/j.ydbio.2018.06.020 UR - https://m2.mtmt.hu/api/publication/30510582 ID - 30510582 N1 - Graduate Program in Life Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, United States Center for Vascular and Inflammatory Diseases and the Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201, United States The Wilmer Eye Institute, Johns Hopkins University School of Medicine, 400N. Broadway M002 P, Baltimore, MD 21231, United States Cited By :19 Export Date: 19 January 2022 CODEN: DEBIA Correspondence Address: Fossett, N.; University of Maryland, 800 W. Baltimore Street, Room 215, United States; email: nfossett@som.umaryland.edu AB - Hematopoietic progenitor choice between multipotency and differentiation is tightly regulated by intrinsic factors and extrinsic signals from the surrounding microenvironment. The Drosophila melanogaster hematopoietic lymph gland has emerged as a powerful tool to investigate mechanisms that regulate hematopoietic progenitor choice in vivo. The lymph gland contains progenitor cells, which share key characteristics with mammalian hematopoietic progenitors such as quiescence, multipotency and niche dependence. The lymph gland is zonally arranged, with progenitors located in medullary zone, differentiating cells in the cortical zone, and the stem cell niche or Posterior Signaling Center (PSC) residing at the base of the medullary zone (MZ). This arrangement facilitates investigations into how signaling from the microenvironment controls progenitor choice. The Drosophila Friend of GATA transcriptional regulator, U-shaped, is a conserved hematopoietic regulator. To identify additional novel intrinsic and extrinsic regulators that interface with U-shaped to control hematopoiesis, we conducted an in vivo screen for factors that genetically interact with u-shaped. Smoothened, a downstream effector of Hedgehog signaling, was one of the factors identified in the screen. Here we report our studies that characterized the relationship between Smoothened and U-shaped. We showed that the PSC and Hedgehog signaling are required for U-shaped expression and that U-shaped is an important intrinsic progenitor regulator. These observations identify a potential link between the progenitor regulatory machinery and extrinsic signals from the PSC. Furthermore, we showed that both Hedgehog signaling and the PSC are required to maintain a subpopulation of progenitors. This led to a delineation of PSC-dependent versus PSC-independent progenitors and provided further evidence that the MZ progenitor population is heterogeneous. Overall, we have identified a connection between a conserved hematopoietic master regulator and a putative stem cell niche, which adds to our understanding of how signals from the microenvironment regulate progenitor multipotency. LA - English DB - MTMT ER - TY - JOUR AU - Bazzi, Wael AU - Cattenoz, Pierre B AU - Delaporte, Claude AU - Dasari, Vasanthi AU - Sakr, Rosy AU - Yuasa, Yoshihiro AU - Giangrande, Angela TI - Embryonic hematopoiesis modulates the inflammatory response and larval hematopoiesis in Drosophila JF - ELIFE J2 - ELIFE VL - 7 PY - 2018 PG - 30 SN - 2050-084X DO - 10.7554/eLife.34890.001 UR - https://m2.mtmt.hu/api/publication/27602855 ID - 27602855 AB - Recent lineage tracing analyses have significantly improved our understanding of immune system development and highlighted the importance of the different hematopoietic waves. The current challenge is to understand whether these waves interact and whether this affects the function of the immune system. Here we report a molecular pathway regulating the immune response and involving the communication between embryonic and larval hematopoietic waves in Drosophila. Down-regulating the transcription factor Gcm specific to embryonic hematopoiesis enhances the larval phenotypes induced by over-expressing the pro-inflammatory Jak/Stat pathway or by wasp infestation. Gcm works by modulating the transduction of the Upd cytokines to the site of larval hematopoiesis and hence the response to chronic Jak/Stat overexpression and acute wasp infestation immune challenges. Thus, homeostatic interactions control the function of the immune system in physiology and pathology. Our data also indicate that a transiently expressed developmental pathway has a long-lasting effect on the immune response. © Bazzi et al. LA - English DB - MTMT ER - TY - JOUR AU - Boulet, Manon AU - Miller, Marion AU - Vandel, Laurence AU - Waltzer, Lucas TI - From Drosophila Blood Cells to Human Leukemia JF - ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY J2 - ADV EXP MED BIOL VL - 1076 PY - 2018 SP - 195 EP - 214 PG - 20 SN - 0065-2598 DO - 10.1007/978-981-13-0529-0_11 UR - https://m2.mtmt.hu/api/publication/30510499 ID - 30510499 N1 - Cited By :8 Export Date: 15 February 2024 CODEN: AEMBA Correspondence Address: Waltzer, L.; Université Clermont Auvergne, France; email: lucas.waltzer@uca.fr AB - The hematopoietic system plays a critical role in establishing the proper response against invading pathogens or in removing cancerous cells. Furthermore, deregulations of the hematopoietic differentiation program are at the origin of numerous diseases including leukemia. Importantly, many aspects of blood cell development have been conserved from human to Drosophila. Hence, Drosophila has emerged as a potent genetic model to study blood cell development and leukemia in vivo. In this chapter, we give a brief overview of the Drosophila hematopoietic system, and we provide a protocol for the dissection and the immunostaining of the larval lymph gland, the most studied hematopoietic organ in Drosophila. We then focus on the various paradigms that have been used in fly to investigate how conserved genes implicated in leukemogenesis control blood cell development. Specific examples of Drosophila models for leukemia are presented, with particular attention to the most translational ones. Finally, we discuss some limitations and potential improvements of Drosophila models for studying blood cell cancer. LA - English DB - MTMT ER - TY - JOUR AU - Hiroyasu, Aoi AU - DeWitt, David C. AU - Goodman, Alan G. TI - Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis JF - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS J2 - JOVE-J VIS EXP PY - 2018 IS - 135 PG - 12 SN - 1940-087X DO - 10.3791/57077 UR - https://m2.mtmt.hu/api/publication/30425005 ID - 30425005 N1 - Export Date: 12 October 2021 AB - During the pathogenic infection of Drosophila melanogaster, hemocytes play an important role in the immune response throughout the infection. Thus, the goal of this protocol is to develop a method to visualize the pathogen invasion in a specific immune compartment of flies, namely hemocytes. Using the method presented here, up to 3 x 10(6) live hemocytes can be obtained from 200 Drosophila 3rd instar larvae in 30 min for ex vivo infection. Alternatively, hemocytes can be infected in vivo through injection of 3rd instar larvae followed by hemocyte extraction up to 24 h post-infection. These infected primary cells were fixed, stained, and imaged using confocal microscopy. Then, 3D representations were generated from the images to definitively show pathogen invasion. Additionally, high-quality RNA for qRT-PCR can be obtained for the detection of pathogen mRNA following infection, and sufficient protein can be extracted from these cells for Western blot analysis. Taken together, we present a method for definite reconciliation of pathogen invasion and confirmation of infection using bacterial and viral pathogen types and an efficient method for hemocyte extraction to obtain enough live hemocytes from Drosophila larvae for ex vivo and in vivo infection experiments. LA - English DB - MTMT ER - TY - JOUR AU - Mandal, L. AU - Ghosh, S. AU - Mandal, S. TI - Detecting proliferation of adult hemocytes in Drosophila by BrdU incorporation [version 1; referees: 2 approved] JF - WELLCOME OPEN RESEARCH J2 - WELLCOME OPEN RESEARCH VL - 3 PY - 2018 SN - 2398-502X DO - 10.12688/wellcomeopenres.14560.1 UR - https://m2.mtmt.hu/api/publication/31149601 ID - 31149601 N1 - Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-Mohali, Manauli, Punjab, 140306, India Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-Mohali, Manauli, Punjab, 140306, India Export Date: 31 January 2020 Correspondence Address: Mandal, L.; Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-MohaliIndia; email: lolitika@iisermohali.ac.in Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-Mohali, Manauli, Punjab, 140306, India Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research-Mohali, Manauli, Punjab, 140306, India Cited By :1 Export Date: 12 February 2021 Correspondence Address: Mandal, L.; Developmental Genetics Laboratory, India; email: lolitika@iisermohali.ac.in AB - Drosophila and mammalian hematopoiesis share several similarities that ranges from phases to the battery of transcription factors and signaling molecules that execute this process. These resounding similarities along with the rich genetic tools available in fruitfly makes it a popular invertebrate model to study blood cell development both during normal and aberrant conditions. The larval system is the most extensively studied to date. Several studies have shown that these hemocytes just like mammalian counterpart proliferate and get routinely regenerated upon infection. However, employing the same protocol it was concluded that blood cell proliferation although abundant in larval stages is absent in adult fruitfly. The current protocol describes the strategies that can be employed to document the hemocyte proliferation in adulthood. The fact that a subset of blood cells tucked away in the hematopoietic hub are not locked in senescence, rather they still harbour the proliferative capacity to tide over challenges was successfully demonstrated by this method. Although we have adopted bacterial infection as a bait to evoke this proliferative capacity of the hemocytes, we envision that it can also efficiently characterize the proliferative responses of hemocytes in tumorigenic conditions as well as scenarios of environmental and metabolic stresses during adulthood. © 2018 Ghosh S et al. LA - English DB - MTMT ER - TY - JOUR AU - Mohamed, Amr A AU - Ali, Mona M AU - Dorrah, Moataza A AU - Bassal, Taha T M TI - Mediation of inducible nitric oxide and immune-reactive lysozymes biosynthesis by eicosanoid and biogenic amines in flesh flies JF - INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE J2 - INT J TROP INSECT SC VL - 38 PY - 2018 IS - 1 SP - 93 EP - 104 PG - 12 SN - 1742-7584 DO - 10.1017/S1742758417000315 UR - https://m2.mtmt.hu/api/publication/27347640 ID - 27347640 N1 - Cited By :14 Export Date: 19 January 2022 LA - English DB - MTMT ER - TY - JOUR AU - Tokusumi, Yumiko AU - Tokusumi, Tsuyoshi AU - Schulz, Robert A TI - Mechanical stress to Drosophila larvae stimulates a cellular immune response through the JAK/STAT signaling pathway JF - BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS J2 - BIOCHEM BIOPH RES CO VL - 502 PY - 2018 IS - 3 SP - 415 EP - 421 PG - 7 SN - 0006-291X DO - 10.1016/j.bbrc.2018.05.192 UR - https://m2.mtmt.hu/api/publication/27602854 ID - 27602854 N1 - Cited By :10 Export Date: 19 January 2022 CODEN: BBRCA Correspondence Address: Schulz, R.A.; Department of Biological Sciences, United States; email: rschulz@nd.edu LA - English DB - MTMT ER - TY - JOUR AU - Anderson, Abigail M AU - Bailetti, Alessandro A AU - Rodkin, Elizabeth AU - De, Atish AU - Bach, Erika A TI - A Genetic Screen Reveals an Unexpected Role for Yorkie Signaling in JAK/STAT-Dependent Hematopoietic Malignancies in Drosophila melanogaster JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 7 PY - 2017 IS - 8 SP - 2427 EP - 2438 PG - 12 SN - 2160-1836 DO - 10.1534/g3.117.044172 UR - https://m2.mtmt.hu/api/publication/26891561 ID - 26891561 N1 - Megjegyzés-26822334 OA gold LA - English DB - MTMT ER - TY - CHAP AU - Brüser, Lena AU - Bogdan, Sven ED - Jockusch, BM TI - Molecular Control of Actin Dynamics In Vivo: Insights from Drosophila T2 - The Actin Cytoskeleton PB - Springer Netherlands CY - Cham (Németország) SN - 9783319463711 T3 - Handbook of Experimental Pharmacology, ISSN 0171-2004 ; 235. PB - Springer Netherlands PY - 2017 SP - 285 EP - 310 PG - 26 DO - 10.1007/164_2016_33 UR - https://m2.mtmt.hu/api/publication/26274581 ID - 26274581 N1 - Cited By :7 Export Date: 17 October 2023 Correspondence Address: Bogdan, S.; Institute for Neurobiology, Badestrasse 9, Germany; email: sbogdan@uni-muenster.de LA - English DB - MTMT ER - TY - JOUR AU - Del Signore, Steven J AU - Biber, Sarah A AU - Lehmann, Katherine S AU - Heimler, Stephanie R AU - Rosenfeld, Benjamin H AU - Eskin, Tania L AU - Sweeney, Sean T AU - Rodal, Avital A TI - dOCRL maintains immune cell quiescence by regulating endosomal traffic JF - PLOS GENETICS J2 - PLOS GENET VL - 13 PY - 2017 IS - 10 PG - 29 SN - 1553-7390 DO - 10.1371/journal.pgen.1007052 UR - https://m2.mtmt.hu/api/publication/27055182 ID - 27055182 N1 - Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA, United States Department of Biology, University of York, York, United Kingdom Cited By :6 Export Date: 4 May 2021 Correspondence Address: Rodal, A.A.; Rosenstiel Basic Medical Sciences Research Center, United States; email: arodal@brandeis.edu LA - English DB - MTMT ER - TY - JOUR AU - Dostalova, Anna AU - Rommelaere, Samuel AU - Poidevin, Mickael AU - Lemaitre, Bruno TI - Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps JF - BMC BIOLOGY J2 - BMC BIOL VL - 15 PY - 2017 PG - 16 SN - 1741-7007 DO - 10.1186/s12915-017-0408-0 UR - https://m2.mtmt.hu/api/publication/26891459 ID - 26891459 N1 - Global Health Institute, School of Life Sciences, École Polytechnique Fédérale Lausanne (EPFL), Lausanne, CH-1015, Switzerland Institute for Integrative Biology of the Cell, Université Paris-Saclay, CEA, CNRS, Université Paris Sud, 1 Avenue de la Terrasse, Gif-sur-Yvette, 91198, France Cited By :40 Export Date: 19 January 2022 Correspondence Address: Dostálová, A.; Global Health Institute, Switzerland; email: anna.svarovska@gmail.com LA - English DB - MTMT ER - TY - JOUR AU - El, Chamy Laure AU - Matt, Nicolas AU - Reichhart, Jean-Marc TI - Advances in Myeloid-Like Cell Origins and Functions in the Model Organism Drosophila melanogaster JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 5 PY - 2017 IS - 1 PG - 17 SN - 2165-0497 DO - 10.1128/microbiolspec.MCHD-0038-2016 UR - https://m2.mtmt.hu/api/publication/26536389 ID - 26536389 N1 - Laboratoire de Génétique de la drosophile et virulence microbienne, UR. EGFEM, Faculté des Sciences, Université Saint-Joseph de Beyrouth, B.P. 17-5208, Mar Mikhaël Beyrouth, 1104 2020, Lebanon Université de Strasbourg, UPR 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg Cedex, 67084, France Cited By :3 Export Date: 19 January 2022 Correspondence Address: El Chamy, L.; Laboratoire de Génétique de la drosophile et virulence microbienne, B.P. 17-5208, Lebanon; email: Laure.chamy@usj.edu.lb LA - English DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Török, Tibor AU - Medzihradszky F., Katalin AU - Darula, Zsuzsanna AU - Andó, István AU - Kurucz, Judit Éva TI - Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 76 PY - 2017 SP - 403 EP - 411 PG - 9 SN - 0145-305X DO - 10.1016/j.dci.2017.07.013 UR - https://m2.mtmt.hu/api/publication/3249751 ID - 3249751 N1 - Megjegyzés-27090701 Megjegyzés-26822333 OA No LA - English DB - MTMT ER - TY - JOUR AU - Hughes, S AU - Woollard, A TI - RUNX in invertebrates JF - ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY J2 - ADV EXP MED BIOL VL - 962 PY - 2017 SP - 3 EP - 18 PG - 16 SN - 0065-2598 DO - 10.1007/978-981-10-3233-2_1 UR - https://m2.mtmt.hu/api/publication/26674050 ID - 26674050 LA - English DB - MTMT ER - TY - JOUR AU - Khadilkar, Rohan J AU - Vogl, Wayne AU - Goodwin, Katharine AU - Tanentzapf, Guy TI - Modulation of occluding junctions alters the hematopoietic niche to trigger immune activation JF - ELIFE J2 - ELIFE VL - 6 PY - 2017 PG - 30 SN - 2050-084X DO - 10.7554/eLife.28081 UR - https://m2.mtmt.hu/api/publication/26891460 ID - 26891460 N1 - Cited By :16 Export Date: 14 June 2022 LA - English DB - MTMT ER - TY - JOUR AU - Louradour, Isabelle AU - Sharma, Anurag AU - Morin-Poulard, Ismael AU - Letourneau, Manon AU - Vincent, Alain AU - Crozatier, Michele AU - Vanzo, Nathalie TI - Reactive oxygen species-dependent Toll/NF-kappa B activation in the Drosophila hematopoietic niche confers resistance to wasp parasitism JF - ELIFE J2 - ELIFE VL - 6 PY - 2017 PG - 22 SN - 2050-084X DO - 10.7554/eLife.25496 UR - https://m2.mtmt.hu/api/publication/27260897 ID - 27260897 N1 - Centre de Biologie du Développement, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France National Institutes of Health, Bethesda, United States Department of Biomedical Sciences, NU Centre for Science Education and Research, Nitte University, Mangalore, India Hubrecht Institute, Utrecht, Netherlands Cited By :35 Export Date: 19 January 2022 Correspondence Address: Crozatier, M.; Centre de Biologie du Développement, France; email: michele.crozatier-borde@univ-tlse3.fr LA - English DB - MTMT ER - TY - JOUR AU - Parker, BJ AU - Barribeau, SM AU - Laughton, AM AU - Griffin, LH AU - Gerardo, NM TI - Life-history strategy determines constraints on immune function JF - JOURNAL OF ANIMAL ECOLOGY J2 - J ANIM ECOL VL - 86 PY - 2017 IS - 3 SP - 473 EP - 483 PG - 11 SN - 0021-8790 DO - 10.1111/1365-2656.12657 UR - https://m2.mtmt.hu/api/publication/26674052 ID - 26674052 N1 - N1 Funding details: Emory University N1 Funding details: NIH, National Institutes of Health N1 Funding details: 31003A-116057, NSF, National Science Foundation N1 Funding details: 31003A-116057, NSF, National Sleep Foundation N1 Funding details: DBI-1306387, NSF, National Science Foundation N1 Funding details: DBI-1306387, NSF, National Sleep Foundation N1 Funding details: IOS-1025853, NSF, National Science Foundation N1 Funding details: IOS-1025853, NSF, National Sleep Foundation N1 Funding text: Members of the Gerardo laboratory, J. Brisson, N. Moran and two anonymous reviewers provided valuable feedback on drafts of this manuscript. This work was supported by NSF grant IOS-1025853 to N.M.G. B.J.P. was supported by a graduate research fellowship and NSF grant DBI-1306387. S.M.B. was supported by the Swiss NSF (# 31003A-116057 to Paul Schmid-Hempel). L.G. was supported by Emory University's NIH IRACDA FIRST Postdoctoral Program. LA - English DB - MTMT ER - TY - JOUR AU - Tokusumi, Tsuyoshi AU - Tokusumi, Yumiko AU - Brahier, Mark S AU - Lam, Victoria AU - Stoller-Conrad, Jessica R AU - Kroeger, Paul T AU - Schulz, Robert A TI - Screening and Analysis of Janelia FlyLight Project Enhancer-Gal4 Strains Identifies Multiple Gene Enhancers Active During Hematopoiesis in Normal and Wasp-Challenged Drosophila Larvae JF - G3-GENES GENOMES GENETICS J2 - G3-GENES GENOM GENET VL - 7 PY - 2017 IS - 2 SP - 437 EP - 448 PG - 12 SN - 2160-1836 DO - 10.1534/g3.116.034439 UR - https://m2.mtmt.hu/api/publication/26536387 ID - 26536387 N1 - Cited By :12 Export Date: 19 January 2022 Correspondence Address: Schulz, R.A.; Department of Biological Sciences, 147 Galvin Life Science Building, United States; email: rschulz@nd.edu LA - English DB - MTMT ER - TY - JOUR AU - Wang, Pan AU - Zhuo, Xiao-Rong AU - Tang, Lin AU - Liu, Xu-Sheng AU - Wang, Yu-Feng AU - Wang, Guo-Xiu AU - Yu, Xiao-Qiang AU - Wang, Jia-Lin TI - C-type lectin interacting with beta-integrin enhances hemocytic encapsulation in the cotton bollworm, Helicoverpa armigera JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 86 PY - 2017 SP - 29 EP - 40 PG - 12 SN - 0965-1748 DO - 10.1016/j.ibmb.2017.05.005 UR - https://m2.mtmt.hu/api/publication/26714786 ID - 26714786 LA - English DB - MTMT ER - TY - JOUR AU - Wang, Weilin AU - Li, Meijia AU - Wang, Lingling AU - Chen, Hao AU - Liu, Zhaoqun AU - Jia, Zhihao AU - Qiu, Limei AU - Song, Linsheng TI - The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 67 PY - 2017 SP - 221 EP - 228 PG - 8 SN - 0145-305X DO - 10.1016/j.dci.2016.09.017 UR - https://m2.mtmt.hu/api/publication/26377205 ID - 26377205 LA - English DB - MTMT ER - TY - JOUR AU - Wood, Will AU - Martin, Paul TI - Macrophage Functions in Tissue Patterning and Disease: New Insights from the Fly JF - DEVELOPMENTAL CELL J2 - DEV CELL VL - 40 PY - 2017 IS - 3 SP - 221 EP - 233 PG - 13 SN - 1534-5807 DO - 10.1016/j.devcel.2017.01.001 UR - https://m2.mtmt.hu/api/publication/26698809 ID - 26698809 N1 - Department of Cellular and Molecular Medicine, Biomedical Sciences, University of Bristol, Bristol, BS8 1TD, United Kingdom Departments of Biochemistry and Physiology, Pharmacology and Neuroscience, Biomedical Sciences, University of Bristol, Bristol, BS8 1TD, United Kingdom School of Medicine, Cardiff University, Cardiff, CF14 4XN, United Kingdom Lee Kong Chiang School of Medicine, Nanyang Technological University, Singapore, 636921, Singapore Cited By :44 Export Date: 19 January 2022 CODEN: DCEEB Correspondence Address: Wood, W.; Department of Cellular and Molecular Medicine, United Kingdom; email: w.wood@bristol.ac.uk LA - English DB - MTMT ER - TY - JOUR AU - Zhang, Chen U AU - Cadigan, Ken M TI - The matrix protein Tiggrin regulates plasmatocyte maturation in Drosophila larva JF - DEVELOPMENT J2 - DEVELOPMENT VL - 144 PY - 2017 IS - 13 SP - 2415 EP - 2427 PG - 13 SN - 0950-1991 DO - 10.1242/dev.149641 UR - https://m2.mtmt.hu/api/publication/26878576 ID - 26878576 N1 - Megjegyzés-26674049 N1 Funding details: G012896, U-M, University of Michigan N1 Funding details: GM108468, NIH, National Institutes of Health N1 Funding text: This work was funded by grants from the National Institutes of Health (GM108468), American Heart Association (12PRE9520018) and the University of Michigan Comprehensive Cancer Center (G012896). Deposited in PMC for release after 12 months. Megjegyzés-26674059 N1 Funding details: G012896, U-M, University of Michigan N1 Funding details: GM108468, NIH, National Institutes of Health N1 Funding text: This work was funded by grants from the National Institutes of Health (GM108468), American Heart Association (12PRE9520018) and the University of Michigan Comprehensive Cancer Center (G012896). Deposited in PMC for release after 12 months. Megjegyzés-26674062 N1 Funding details: G012896, U-M, University of Michigan N1 Funding details: GM108468, NIH, National Institutes of Health N1 Funding text: This work was funded by grants from the National Institutes of Health (GM108468), American Heart Association (12PRE9520018) and the University of Michigan Comprehensive Cancer Center (G012896). Deposited in PMC for release after 12 months. Megjegyzés-26674033 N1 Funding details: G012896, U-M, University of Michigan N1 Funding details: GM108468, NIH, National Institutes of Health N1 Funding text: This work was funded by grants from the National Institutes of Health (GM108468), American Heart Association (12PRE9520018) and the University of Michigan Comprehensive Cancer Center (G012896). Deposited in PMC for release after 12 months. LA - English DB - MTMT ER - TY - JOUR AU - Anderl, I AU - Vesala, L AU - Ihalainen, TO AU - Vanha-Aho, LM AU - Andó, István AU - Ramet, M AU - Hultmark, D TI - Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection. JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 12 PY - 2016 IS - 7 SP - e1005746 SN - 1553-7366 DO - 10.1371/journal.ppat.1005746 UR - https://m2.mtmt.hu/api/publication/3096913 ID - 3096913 N1 - WoS:hiba:000383366400030 2019-03-03 19:41 első oldal nem egyezik AB - Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail. LA - English DB - MTMT ER - TY - JOUR AU - Cattenoz, Pierre B AU - Giangrande, Angela TI - Revisiting the role of the Gcm transcription factor, from master regulator to Swiss army knife JF - FLY J2 - FLY VL - 10 PY - 2016 IS - 4 SP - 210 EP - 218 PG - 9 SN - 1933-6934 DO - 10.1080/19336934.2016.1212793 UR - https://m2.mtmt.hu/api/publication/26203670 ID - 26203670 LA - English DB - MTMT ER - TY - JOUR AU - Dey, Nidhi Sharma AU - Ramesh, Parvathy AU - Chugh, Mayank AU - Mandal, Sudip AU - Mandal, Lolitika TI - Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila JF - ELIFE J2 - ELIFE VL - 5 PY - 2016 PG - 24 SN - 2050-084X DO - 10.7554/eLife.18295 UR - https://m2.mtmt.hu/api/publication/26377269 ID - 26377269 N1 - Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India Center for Plant Molecular Biology, University of Tuebingen, Tuebingen, Germany Cited By :31 Export Date: 19 January 2022 Correspondence Address: Mandal, L.; Developmental Genetics Laboratory, India; email: lolitika@iisermohali.ac.in LA - English DB - MTMT ER - TY - JOUR AU - Gao, Hongjuan AU - Baldeosingh, Rajkumar AU - Wu, Xiaorong AU - Fossett, Nancy TI - The Friend of GATA Transcriptional Co-Regulator, U-Shaped, Is a Downstream Antagonist of Dorsal-Driven Prohemocyte Differentiation in Drosophila JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 5 PG - 24 SN - 1932-6203 DO - 10.1371/journal.pone.0155372 UR - https://m2.mtmt.hu/api/publication/26016211 ID - 26016211 N1 - Cited By :5 Export Date: 30 June 2022 CODEN: POLNC LA - English DB - MTMT ER - TY - JOUR AU - Giordani, Giorgia AU - Barraco, Marilena AU - Giangrande, Angela AU - Martinelli, Giovanni AU - Guadagnuolo, Viviana AU - Simonetti, Giorgia AU - Perini, Giovanni AU - Bernardoni, Roberto TI - The human Smoothened inhibitor PF-04449913 induces exit from quiescence and loss of multipotent Drosophila hematopoietic progenitor cells JF - ONCOTARGET J2 - ONCOTARGET VL - 7 PY - 2016 IS - 34 SP - 55313 EP - 55327 PG - 15 SN - 1949-2553 DO - 10.18632/oncotarget.10879 UR - https://m2.mtmt.hu/api/publication/26203994 ID - 26203994 N1 - Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Bologna, Italy Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch, 67404, France Department of Experimental, Diagnostic and Specialty Medicine (DIMES), Institute of Hematology 'L. e A. Seràgnoli', University of Bologna, Bologna, Italy Health Sciences and Technology - Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Ozzano Emilia, Italy Department of Biological Sciences, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, United Kingdom Institute of Hematology, 'L e A Seràgnoli', S. Orsola-Malpighi Hospital, Bologna, Italy Cited By :14 Export Date: 19 January 2022 Correspondence Address: Bernardoni, R.; Department of Pharmacy and Biotechnology (FaBiT), Italy; email: roberto.bernardoni@unibo.it LA - English DB - MTMT ER - TY - JOUR AU - Hillyer, Julian F TI - Insect immunology and hematopoiesis JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 58 PY - 2016 SP - 102 EP - 118 PG - 17 SN - 0145-305X DO - 10.1016/j.dci.2015.12.006 UR - https://m2.mtmt.hu/api/publication/25771630 ID - 25771630 N1 - Cited By :187 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Hillyer, J.F.; Department of Biological Sciences, VU Station B 35-1634, United States; email: julian.hillyer@vanderbilt.edu LA - English DB - MTMT ER - TY - JOUR AU - Kari, Beáta AU - Csordás, Gábor AU - Honti, Viktor AU - Cinege, Gyöngyi Ilona AU - Williams, MJ AU - Andó, István AU - Kurucz, Judit Éva TI - The raspberry Gene Is Involved in the Regulation of the Cellular Immune Response in Drosophila melanogaster JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 3 PG - 13 SN - 1932-6203 DO - 10.1371/journal.pone.0150910 UR - https://m2.mtmt.hu/api/publication/3045263 ID - 3045263 N1 - Biological Research Centre of Hungarian Academy of Sciences, Immunology Unit, Institute of Genetics, Szeged, Hungary Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden Cited By :6 Export Date: 14 February 2021 CODEN: POLNC AB - Drosophila is an extremely useful model organism for understanding how innate immune mechanisms defend against microbes and parasitoids. Large foreign objects trigger a potent cellular immune response in Drosophila larva. In the case of endoparasitoid wasp eggs, this response includes hemocyte proliferation, lamellocyte differentiation and eventual encapsulation of the egg. The encapsulation reaction involves the attachment and spreading of hemocytes around the egg, which requires cytoskeletal rearrangements, changes in adhesion properties and cell shape, as well as melanization of the capsule. Guanine nucleotide metabolism has an essential role in the regulation of pathways necessary for this encapsulation response. Here, we show that the Drosophila inosine 5'-monophosphate dehydrogenase (IMPDH), encoded by raspberry (ras), is centrally important for a proper cellular immune response against eggs from the parasitoid wasp Leptopilina boulardi. Notably, hemocyte attachment to the egg and subsequent melanization of the capsule are deficient in hypomorphic ras mutant larvae, which results in a compromised cellular immune response and increased survival of the parasitoid. LA - English DB - MTMT ER - TY - JOUR AU - League, Garrett P AU - Hillyer, Julian F TI - Functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts JF - BMC BIOLOGY J2 - BMC BIOL VL - 14 PY - 2016 PG - 17 SN - 1741-7007 DO - 10.1186/s12915-016-0305-y UR - https://m2.mtmt.hu/api/publication/26203993 ID - 26203993 N1 - Cited By :20 Export Date: 30 June 2022 LA - English DB - MTMT ER - TY - JOUR AU - Letourneau, Manon AU - Lapraz, Francois AU - Sharma, Anurag AU - Vanzo, Nathalie AU - Waltzer, Lucas AU - Crozatier, Michele TI - Drosophila hematopoiesis under normal conditions and in response to immune stress JF - FEBS LETTERS J2 - FEBS LETT VL - 590 PY - 2016 IS - 22 SP - 4034 EP - 4051 PG - 18 SN - 0014-5793 DO - 10.1002/1873-3468.12327 UR - https://m2.mtmt.hu/api/publication/26377268 ID - 26377268 N1 - Megjegyzés-26322880 N1 Funding details: ANR, Agence Nationale de la Recherche N1 Funding details: ARC, Association pour la Recherche sur le Cancer N1 Funding details: CNRS, Centre National de la Recherche Scientifique N1 Funding details: IFCPAR, Indo-French Centre for the Promotion of Advanced Research N1 Funding text: We thank M. Meister and C. Monod for critical reading of the manuscript. Work in the authors’ laboratory is supported by the CNRS, University Toulouse III, Ministère de la Recherche (ANR « programme blanc »), ARC (Association pour la Recherche sur le Cancer) and CEFIPRA (Centre Franco-Indien pour la Promotion de la Recherche Avancée). ML, FL, AS, NV, LW and MC wrote the manuscript. ML made the figures, LW & MC designed the review. LA - English DB - MTMT ER - TY - JOUR AU - Lőrincz, Péter AU - Lakatos, Zsolt AU - Varga, Ágnes AU - Maruzs, Tamás AU - Simon-Vecsei, Zsófia Judit AU - Darula, Zsuzsanna AU - Benkő, Péter AU - Csordás, Gábor AU - Lippai, Mónika AU - Andó, István AU - Hegedűs, Krisztina AU - Medzihradszky F., Katalin AU - Takáts, Szabolcs AU - Juhász, Gábor TI - MiniCORVET is a Vps8-containing early endosomal tether in Drosophila JF - ELIFE J2 - ELIFE VL - 5 PY - 2016 PG - 27 SN - 2050-084X DO - 10.7554/eLife.14226 UR - https://m2.mtmt.hu/api/publication/30901168 ID - 30901168 N1 - Cited By :33 Export Date: 30 June 2022 AB - Yeast studies identified two heterohexameric tethering complexes, which consist of 4 shared (Vps11, Vps16, Vps18 and Vps33) and 2 specific subunits: Vps3 and Vps8 (CORVET) versus Vps39 and Vps41 (HOPS). CORVET is an early and HOPS is a late endosomal tether. The function of HOPS is well known in animal cells, while CORVET is poorly characterized. Here we show that Drosophila Vps8 is highly expressed in hemocytes and nephrocytes, and localizes to early endosomes despite the lack of a clear Vps3 homolog. We find that Vps8 forms a complex and acts together with Vps16A, Dor/Vps18 and Car/Vps33A, and loss of any of these proteins leads to fragmentation of endosomes. Surprisingly, Vps11 deletion causes enlargement of endosomes, similar to loss of the HOPS-specific subunits Vps39 and Lt/Nps41. We thus identify a 4 subunit-containing miniCORVET complex as an unconventional early endosomal tether in Drosophila. LA - English DB - MTMT ER - TY - JOUR AU - Paredes, Juan C AU - Herren, Jeremy K AU - Schupfer, Fanny AU - Lemaitre, Bruno TI - The Role of Lipid Competition for Endosymbiont-Mediated Protection against Parasitoid Wasps in Drosophila JF - MBIO J2 - MBIO VL - 7 PY - 2016 IS - 4 PG - 8 SN - 2161-2129 DO - 10.1128/mBio.01006-16 UR - https://m2.mtmt.hu/api/publication/26203667 ID - 26203667 LA - English DB - MTMT ER - TY - JOUR AU - Parsons, Brendon AU - Foley, Edan TI - Cellular immune defenses of Drosophila melanogaster JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 58 PY - 2016 SP - 95 EP - 101 PG - 7 SN - 0145-305X DO - 10.1016/j.dci.2015.12.019 UR - https://m2.mtmt.hu/api/publication/25771631 ID - 25771631 N1 - Cited By :34 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Foley, E.; Department of Medical Microbiology and Immunology, Canada; email: efoley@ualberta.ca LA - English DB - MTMT ER - TY - JOUR AU - Reimels, TA AU - Pfleger, CM TI - Methods to examine the lymph gland and hemocytes in Drosophila larvae JF - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS J2 - JOVE-J VIS EXP VL - 2016 PY - 2016 IS - 117 SN - 1940-087X DO - 10.3791/54544 UR - https://m2.mtmt.hu/api/publication/26674053 ID - 26674053 N1 - N1 Funding details: 1257939, NSF, National Science Foundation N1 Funding details: R01CA140451, NCI, National Cancer Institute N1 Funding details: R01CA140451, NIH, National Institutes of Health N1 Funding details: T32CA078207, NCI, National Cancer Institute N1 Funding details: T32CA078207, NIH, National Institutes of Health N1 Funding details: W81XWH-14-1-0059, DOD, U.S. Department of Defense N1 Funding text: We thank Matthew O'Connell, Maryam Jahanshahi, and Andreas Jenny for assistance. We thank István Andó for plasmatocyte-specific antibodies, Utpal Banerjee for dome-meso-EBFP2 flies, Julian Martinez-Agosto for antp>GFP flies, and Michael O'Connor for ptth and ptth>grim flies. These methods were developed with support by the Kimmel Foundation, the Leukemia & Lymphoma Society, NIH/NCI R01CA140451, NSF 1257939, DOD/NFRP W81XWH-14-1-0059, and NIH/NCI T32CA078207. Megjegyzés-26295705 N1 Funding details: 1257939, NSF, National Science Foundation N1 Funding details: R01CA140451, NCI, National Cancer Institute N1 Funding details: R01CA140451, NIH, National Institutes of Health N1 Funding details: T32CA078207, NCI, National Cancer Institute N1 Funding details: T32CA078207, NIH, National Institutes of Health N1 Funding details: W81XWH-14-1-0059, DOD, U.S. Department of Defense LA - English DB - MTMT ER - TY - JOUR AU - Schmid, Martin R AU - Anderl, Ines AU - Vo, Hoa T M AU - Valanne, Susanna AU - Yang, Hairu AU - Kronhamn, Jesper AU - Ramet, Mika AU - Rusten, Tor Erik AU - Hultmark, Dan TI - Genetic Screen in Drosophila Larvae Links ird1 Function to Toll Signaling in the Fat Body and Hemocyte Motility JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 7 PG - 30 SN - 1932-6203 DO - 10.1371/journal.pone.0159473 UR - https://m2.mtmt.hu/api/publication/26237558 ID - 26237558 N1 - Department of Molecular Biology, Umeå University, Umeå, Sweden BioMediTech, University of Tampere, Tampere, Finland PEDEGO Research Center, Medical Research Center Oulu, University of Oulu, Oulu University Hospital, Oulu, Finland Department of Molecular Cell Biology, Oslo University Hospital, Centre for Cancer Biomedicine, University of Oslo, Oslo, Norway Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden Cited By :6 Export Date: 23 September 2021 CODEN: POLNC LA - English DB - MTMT ER - TY - JOUR AU - Sigle, LT AU - Hillyer, JF TI - Mosquito hemocytes preferentially aggregate and phagocytose pathogens in the periostial regions of the heart that experience the most hemolymph flow JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 55 PY - 2016 SP - 90 EP - 101 PG - 12 SN - 0145-305X DO - 10.1016/j.dci.2015.10.018 UR - https://m2.mtmt.hu/api/publication/25573127 ID - 25573127 LA - English DB - MTMT ER - TY - JOUR AU - Vanha-aho, Leena-Maija AU - Valanne, Susanna AU - Ramet, Mika TI - Cytokines in Drosophila immunity JF - IMMUNOLOGY LETTERS J2 - IMMUNOL LETT VL - 170 PY - 2016 SP - 42 EP - 51 PG - 10 SN - 0165-2478 DO - 10.1016/j.imlet.2015.12.005 UR - https://m2.mtmt.hu/api/publication/25771633 ID - 25771633 LA - English DB - MTMT ER - TY - JOUR AU - Vogelweith, F AU - Moret, Y AU - Monceau, K AU - Thiéry, D AU - Moreau, J TI - The relative abundance of hemocyte types in a polyphagous moth larva depends on diet JF - JOURNAL OF INSECT PHYSIOLOGY J2 - J INSECT PHYSIOL VL - 88 PY - 2016 SP - 33 EP - 39 PG - 7 SN - 0022-1910 DO - 10.1016/j.jinsphys.2016.02.010 UR - https://m2.mtmt.hu/api/publication/25573126 ID - 25573126 LA - English DB - MTMT ER - TY - JOUR AU - Anderl, Ines AU - Hultmark, Dan TI - New ways to make a blood cell JF - ELIFE J2 - ELIFE VL - 4 PY - 2015 PG - 3 SN - 2050-084X DO - 10.7554/eLife.06877 UR - https://m2.mtmt.hu/api/publication/24796091 ID - 24796091 LA - English DB - MTMT ER -