@article{MTMT:34261233, title = {Continuous muscle, glial, epithelial, neuronal, and hemocyte cell lines for Drosophila research}, url = {https://m2.mtmt.hu/api/publication/34261233}, author = {Coleman-Gosser, Nikki and Hu, Yanhui and Raghuvanshi, Shiva and Stitzinger, Shane and Chen, Weihang and Luhur, Arthur and Mariyappa, Daniel and Josifov, Molly and Zelhof, Andrew and Mohr, Stephanie E. and Perrimon, Norbert and Simcox, Amanda}, doi = {10.7554/eLife.85814}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {12}, unique-id = {34261233}, issn = {2050-084X}, abstract = {Expression of activated Ras, Ras(V12), provides Drosophila cultured cells with a proliferation and survival advantage that simplifies the generation of continuous cell lines. Here, we used lineage-restricted Ras(V12) expression to generate continuous cell lines of muscle, glial, and epithelial cell type. Additionally, cell lines with neuronal and hemocyte characteristics were isolated by cloning from cell cultures established with broad Ras(V12) expression. Differentiation with the hormone ecdysone caused maturation of cells from mesoderm lines into active muscle tissue and enhanced dendritic features in neuronal-like lines. Transcriptome analysis showed expression of key cell-type-specific genes and the expected alignment with single-cell sequencing and in situ data. Overall, the technique has produced in vitro cell models with characteristics of glia, epithelium, muscle, nerve, and hemocyte. The cells and associated data are available from the Drosophila Genomic Resource Center.}, keywords = {MUSCLE; Hemocyte; epithelial; Neuronal; Glial; cell-type-specific cell lines}, year = {2023}, eissn = {2050-084X}, orcid-numbers = {Mariyappa, Daniel/0000-0003-4775-1656} } @article{MTMT:34132755, title = {Understanding the diversity and dynamics of in vivo efferocytosis: Insights from the fly embryo}, url = {https://m2.mtmt.hu/api/publication/34132755}, author = {Heron, R. and Amato, C. and Wood, W. and Davidson, A.J.}, doi = {10.1111/imr.13266}, journal-iso = {IMMUNOL REV}, journal = {IMMUNOLOGICAL REVIEWS}, unique-id = {34132755}, issn = {0105-2896}, year = {2023}, eissn = {1600-065X} } @article{MTMT:33555087, title = {A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/33555087}, author = {Kúthy-Sutus, Enikő and Kharrat, Bayan and Gábor, Erika and Csordás, Gábor and Sinka, Rita and Honti, Viktor}, doi = {10.3390/cells12010024}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {12}, unique-id = {33555087}, abstract = {The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo.}, year = {2023}, eissn = {2073-4409}, orcid-numbers = {Kúthy-Sutus, Enikő/0000-0002-1398-4120; Csordás, Gábor/0000-0001-6871-6839; Sinka, Rita/0000-0003-4040-4184} } @article{MTMT:33628955, title = {The Drosophila-parasitizing wasp Leptopilina heterotoma: A comprehensive model system in ecology and evolution}, url = {https://m2.mtmt.hu/api/publication/33628955}, author = {Quicray, M. and Wilhelm, L. and Enriquez, T. and He, S. and Scheifler, M. and Visser, B.}, doi = {10.1002/ece3.9625}, journal-iso = {ECOL EVOL}, journal = {ECOLOGY AND EVOLUTION}, volume = {13}, unique-id = {33628955}, issn = {2045-7758}, abstract = {The parasitoid Leptopilina heterotoma has been used as a model system for more than 70 years, contributing greatly to diverse research areas in ecology and evolution. Here, we synthesized the large body of work on L. heterotoma with the aim to identify new research avenues that could be of interest also for researchers studying other parasitoids and insects. We start our review with a description of typical L. heterotoma characteristics, as well as that of the higher taxonomic groups to which this species belongs. We then continue discussing host suitability and immunity, foraging behaviors, as well as fat accumulation and life histories. We subsequently shift our focus towards parasitoid-parasitoid interactions, including L. heterotoma coexistence within the larger guild of Drosophila parasitoids, chemical communication, as well as mating and population structuring. We conclude our review by highlighting the assets of L. heterotoma as a model system, including its intermediate life history syndromes, the ease of observing and collecting natural hosts and wasps, as well as recent genomic advances. © 2023 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.}, keywords = {LIPIDS; Virulence; fitness; Associative learning; endosymbiont; Sex pheromones; host-parasitoid community}, year = {2023}, eissn = {2045-7758} } @article{MTMT:32524824, title = {Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids}, url = {https://m2.mtmt.hu/api/publication/32524824}, author = {Cinege, Gyöngyi Ilona and Magyar, Lilla Brigitta and Kovács, Attila Lajos and Lerner, Zita and Juhász, Gábor and Lukacsovich, David and Winterer, Jochen and Lukacsovich, Tamás and Hegedűs, Zoltán and Kurucz, Judit Éva and Hultmark, Dan and Földy, Csaba and Andó, István}, doi = {10.1159/000520110}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {14}, unique-id = {32524824}, issn = {1662-811X}, year = {2022}, eissn = {1662-8128}, pages = {335-354}, orcid-numbers = {Juhász, Gábor/0000-0001-8548-8874; Winterer, Jochen/0000-0002-6800-6594; Lukacsovich, Tamás/0000-0001-5908-9861; Hultmark, Dan/0000-0002-6506-5855; Andó, István/0000-0002-4648-9396} } @article{MTMT:33039275, title = {Hematopoietic plasticity mapped in Drosophila and other insects}, url = {https://m2.mtmt.hu/api/publication/33039275}, author = {Hultmark, Dan and Andó, István}, doi = {10.7554/eLife.78906}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {11}, unique-id = {33039275}, issn = {2050-084X}, year = {2022}, eissn = {2050-084X}, orcid-numbers = {Hultmark, Dan/0000-0002-6506-5855; Andó, István/0000-0002-4648-9396} } @article{MTMT:32876726, title = {Intrinsic and Extrinsic Regulation of Hematopoiesis in Drosophila}, url = {https://m2.mtmt.hu/api/publication/32876726}, author = {Koranteng, F. and Cho, B. and Shim, J.}, doi = {10.14348/molcells.2022.2039}, journal-iso = {MOL CELLS}, journal = {MOLECULES AND CELLS}, volume = {45}, unique-id = {32876726}, issn = {1016-8478}, abstract = {Drosophila melanogaster lymph gland, the primary site of hematopoiesis, contains myeloid-like progenitor cells that differentiate into functional hemocytes in the circulation of pupae and adults. Fly hemocytes are dynamic and plastic, and they play diverse roles in the innate immune response and wound healing. Various hematopoietic regulators in the lymph gland ensure the developmental and functional balance between progenitors and mature blood cells. In addition, systemic factors, such as nutrient availability and sensory inputs, integrate environmental variabilities to synchronize the blood development in the lymph gland with larval growth, physiology, and immunity. This review examines the intrinsic and extrinsic factors determining the progenitor states during hemocyte development in the lymph gland and provides new insights for further studies that may extend the frontier of our collective knowledge on hematopoiesis and innate immunity. © 2022, Korean Society for Molecular and Cellular Biology. All rights reserved.}, keywords = {Animals; Adult; PEPTIDE; calcium; DROSOPHILA; DROSOPHILA; HEMOCYTES; stem cell; human; animal; short survey; physiology; innate immunity; nonhuman; larva; larva; sensory stimulation; Wound healing; Drosophila melanogaster; Drosophila melanogaster; Drosophila melanogaster; human cell; adenosine; blood cell; reactive oxygen metabolite; hematopoietic stem cell; 4 aminobutyric acid; HYPOXIA; beta Catenin; malnutrition; platelet derived growth factor; lymph node; Hematopoiesis; Hematopoiesis; Hematopoiesis; succinic acid; Hematopoietic Stem Cells; Notch receptor; uvomorulin; Drosophila Proteins; nutrient availability; Drosophila protein; Pupa; tricarboxylic acid; calcium calmodulin dependent protein kinase II; forkhead transcription factor; frizzled protein; infestation; protein Patched; lymph gland; Drosophila hematopoiesis; hemocyte differentiation; inter-organ regulation; niche regulation; progenitor cell maintenance}, year = {2022}, eissn = {0219-1032}, pages = {101-108} } @article{MTMT:33181589, title = {Health of the black soldier fly and house fly under mass-rearing conditions: innate immunity and the role of the microbiome}, url = {https://m2.mtmt.hu/api/publication/33181589}, author = {Vogel, M. and Shah, P. N. and Voulgari-Kokota, A. and Maistrou, S. and Aartsma, Y. and Beukeboom, L. W. and Salles, J. Falcao and Van Loon, J. J. A. and Dicke, M. and Wertheim, B.}, doi = {10.3920/JIFF2021.0151}, journal-iso = {J INSECTS FOOD FEED}, journal = {Journal of Insects as Food and Feed}, volume = {8}, unique-id = {33181589}, abstract = {Rearing insects for food and feed is a rapidly growing industry, because it provides excellent opportunities for a sustainable approach to animal protein production. Two fly species, the black soldier fly (BSF) and the house fly (HF), naturally live in decaying organic matter (e.g. compost), and can thus be effectively reared on organic rest streams from the food and agricultural industry. The adoption of these insects as mini-livestock on microbially rich substrates, however, requires us to address how we can safeguard insect health under mass-rearing conditions. In this review, we discuss what is known about the innate immunity of insects in general, especially focusing on a comparative approach to current knowledge for the two dipteran species BSF and HE. We also discuss environmental factors that may affect innate immunity in mass-rearing settings, including temperature, insect densities and diet composition. Furthermore, we address the role of the microbiome in insect health and the associations of these fly species with detrimental or beneficial microbes. Finally, we present a perspective on important open scientific questions for optimizing the mass rearing of these insects with respect to their health and welfare.}, keywords = {TEMPERATURE; DENSITY; innate immunity; DIET; microbiome}, year = {2022}, eissn = {2352-4588}, pages = {857-878} } @article{MTMT:32876692, title = {Adaptations and counter-adaptations in Drosophila host–parasitoid interactions: advances in the molecular mechanisms}, url = {https://m2.mtmt.hu/api/publication/32876692}, author = {Wertheim, B.}, doi = {10.1016/j.cois.2022.100896}, journal-iso = {CURR OPIN INSECT SCI}, journal = {CURRENT OPINION IN INSECT SCIENCE}, volume = {51}, unique-id = {32876692}, issn = {2214-5745}, abstract = {Both hosts and parasitoids evolved a diverse array of traits and strategies for their antagonistic interactions, affecting their chances of encounter, attack and survival after parasitoid attack. This review summarizes the recent progress that has been made in elucidating the molecular mechanisms of these adaptations and counter-adaptations in various Drosophila host–parasitoid interactions. For the hosts, it focuses on the neurobiological and genetic control of strategies in Drosophila adults and larvae of avoidance or escape behaviours upon sensing the parasitoids, and the immunological defences involving diverse classes of haemocytes. For the parasitoids, it highlights their behavioural strategies in host finding, as well as the rich variety of venom components that evolved and were partially acquired through horizontal gene transfer. Recent studies revealed the mechanisms by which these venom components manipulate their parasitized hosts in exhibiting escape behaviour to avoid superparasitism, and their counter-strategies to evade or obstruct the hosts’ immunological defences. © 2022 The Author(s)}, year = {2022}, eissn = {2214-5753} } @article{MTMT:32972124, title = {The Dual Functions of a Bracovirus C-Type Lectin in Caterpillar Immune Response Manipulation}, url = {https://m2.mtmt.hu/api/publication/32972124}, author = {Wu, Xiaotong and Wu, Zhiwei and Ye, Xiqian and Pang, Lan and Sheng, Yifeng and Wang, Zehua and Zhou, Yuenan and Zhu, Jiachen and Hu, Rongmin and Zhou, Sicong and Chen, Jiani and Wang, Zhizhi and Shi, Min and Huang, Jianhua and Chen, Xuexin}, doi = {10.3389/fimmu.2022.877027}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {13}, unique-id = {32972124}, issn = {1664-3224}, abstract = {Parasitoids are widespread in natural ecosystems and normally equipped with diverse viral factors to defeat host immune responses. On the other hand, parasitoids can enhance the antibacterial abilities and improve the hypoimmunity traits of parasitized hosts that may encounter pathogenic infections. These adaptive strategies guarantee the survival of parasitoid offspring, yet their underlying mechanisms are poorly understood. Here, we focused on Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella, and found that C. vestalis parasitization decreases the number of host hemocytes, leading to disruption of the encapsulation reaction. We further found that one bracovirus C-type lectin gene, CvBV_28-1, is highly expressed in the hemocytes of parasitized hosts and participates in suppressing the proliferation rate of host hemocytes, which in turn reduces their population and represses the process of encapsulation. Moreover, CvBV_28-1 presents a classical bacterial clearance ability via the agglutination response in a Ca2+-dependent manner in response to gram-positive bacteria. Our study provides insights into the innovative strategy of a parasitoid-derived viral gene that has dual functions to manipulate host immunity for a successful parasitism.}, keywords = {C-TYPE LECTIN; immunosuppression; Hypoimmunity; agglutination; bracovirus; hemocytes proliferation}, year = {2022}, eissn = {1664-3224} } @article{MTMT:33181490, title = {The immune deficiency and c-Jun N-terminal kinase pathways drive the functional integration of the immune and circulatory systems of mosquitoes}, url = {https://m2.mtmt.hu/api/publication/33181490}, author = {Yan, Yan and Sigle, Leah T. and Rinker, David C. and Estevez-Lao, Tania Y. and Capra, John A. and Hillyer, Julian F.}, doi = {10.1098/rsob.220111}, journal-iso = {OPEN BIOL}, journal = {OPEN BIOLOGY}, volume = {12}, unique-id = {33181490}, abstract = {The immune and circulatory systems of animals are functionally integrated. In mammals, the spleen and lymph nodes filter and destroy microbes circulating in the blood and lymph, respectively. In insects, immune cells that surround the heart valves (ostia), called periostial haemocytes, destroy pathogens in the areas of the body that experience the swiftest haemolymph (blood) flow. An infection recruits additional periostial haemocytes, amplifying heart-associated immune responses. Although the structural mechanics of periostial haemocyte aggregation have been defined, the genetic factors that regulate this process remain less understood. Here, we conducted RNA sequencing in the African malaria mosquito, Anopheles gainhiac, and discovered that an infection upregulates multiple components of the immune deficiency (IMD) and c-Jun N-terminal kinase (JNK) pathways in the heart with periostial haemo cytes. This upregulahon is greater in the heart with periostial haemocytes than in the circulating haemocytes or the entire abdomen. RNA interference-based knockdown then showed that the IMD and JNK pathways drive periostial haemocyte aggregation and alter phagocytosis and melanization on the heart, thereby demonstrating that these pathways regulate the functional integration between the immune and circulatory systems. Understanding how insects fight infection lays the foundation for novel strategies that could protect beneficial insects and harm detrimental ones.}, keywords = {PHAGOCYTOSIS; INSECT; HEART; melanization; haemocytes}, year = {2022}, eissn = {2046-2441} } @article{MTMT:33041863, title = {Drosophila Innate Immunity Involves Multiple Signaling Pathways and Coordinated Communication Between Different Tissues}, url = {https://m2.mtmt.hu/api/publication/33041863}, author = {Yu, S. and Luo, F. and Xu, Y. and Zhang, Y. and Jin, L.H.}, doi = {10.3389/fimmu.2022.905370}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {13}, unique-id = {33041863}, issn = {1664-3224}, abstract = {The innate immune response provides the first line of defense against invading pathogens, and immune disorders cause a variety of diseases. The fruit fly Drosophila melanogaster employs multiple innate immune reactions to resist infection. First, epithelial tissues function as physical barriers to prevent pathogen invasion. In addition, macrophage-like plasmatocytes eliminate intruders through phagocytosis, and lamellocytes encapsulate large particles, such as wasp eggs, that cannot be phagocytosed. Regarding humoral immune responses, the fat body, equivalent to the mammalian liver, secretes antimicrobial peptides into hemolymph, killing bacteria and fungi. Drosophila has been shown to be a powerful in vivo model for studying the mechanism of innate immunity and host-pathogen interactions because Drosophila and higher organisms share conserved signaling pathways and factors. Moreover, the ease with which Drosophila genetic and physiological characteristics can be manipulated prevents interference by adaptive immunity. In this review, we discuss the signaling pathways activated in Drosophila innate immunity, namely, the Toll, Imd, JNK, JAK/STAT pathways, and other factors, as well as relevant regulatory networks. We also review the mechanisms by which different tissues, including hemocytes, the fat body, the lymph gland, muscles, the gut and the brain coordinate innate immune responses. Furthermore, the latest studies in this field are outlined in this review. In summary, understanding the mechanism underlying innate immunity orchestration in Drosophila will help us better study human innate immunity-related diseases. Copyright © 2022 Yu, Luo, Xu, Zhang and Jin.}, keywords = {DROSOPHILA; innate immunity; immune response; SIGNALING PATHWAY; Tissue communication}, year = {2022}, eissn = {1664-3224} } @article{MTMT:31743832, title = {There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells}, url = {https://m2.mtmt.hu/api/publication/31743832}, author = {Csordás, Gábor and Gábor, Erika and Honti, Viktor}, doi = {10.1016/j.ydbio.2020.10.006}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {469}, unique-id = {31743832}, issn = {0012-1606}, year = {2021}, eissn = {1095-564X}, pages = {135-143}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839} } @article{MTMT:32361576, title = {Haemocyte-mediated immunity in insects: Cells, processes and associated components in the fight against pathogens and parasites}, url = {https://m2.mtmt.hu/api/publication/32361576}, author = {Eleftherianos, Ioannis and Heryanto, Christa and Bassal, Taha and Zhang, Wei and Tettamanti, Gianluca and Mohamed, Amr}, doi = {10.1111/imm.13390}, journal-iso = {IMMUNOLOGY}, journal = {IMMUNOLOGY}, volume = {164}, unique-id = {32361576}, issn = {0019-2805}, abstract = {The host defence of insects includes a combination of cellular and humoral responses. The cellular arm of the insect innate immune system includes mechanisms that are directly mediated by haemocytes (e.g., phagocytosis, nodulation and encapsulation). In addition, melanization accompanying coagulation, clot formation and wound healing, nodulation and encapsulation processes leads to the formation of cytotoxic redox-cycling melanin precursors and reactive oxygen and nitrogen species. However, demarcation between cellular and humoral immune reactions as two distinct categories is not straightforward. This is because many humoral factors affect haemocyte functions and haemocytes themselves are an important source of many humoral molecules. There is also a considerable overlap between cellular and humoral immune functions that span from recognition of foreign intruders to clot formation. Here, we review these immune reactions starting with the cellular mechanisms that limit haemolymph loss and participate in wound healing and clot formation and advancing to cellular functions that are critical in restricting pathogen movement and replication. This information is important because it highlights that insect cellular immunity is controlled by a multilayered system, different components of which are activated by different pathogens or during the different stages of the infection.}, keywords = {haematopoiesis; haemocytes; prophenoloxidase; autophagy and apoptosis; cytotoxic intermediates; insect cellular immunity}, year = {2021}, eissn = {1365-2567}, pages = {401-432}, orcid-numbers = {Eleftherianos, Ioannis/0000-0002-4822-3110; Mohamed, Amr/0000-0003-2788-5534} } @article{MTMT:32362075, title = {A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience}, url = {https://m2.mtmt.hu/api/publication/32362075}, author = {Evans, Cory J. and Olson, John M. and Mondal, Bama Charan and Kandimalla, Pratyush and Abbasi, Ariano and Abdusamad, Mai M. and Acosta, Osvaldo and Ainsworth, Julia A. and Akram, Haris M. and Albert, Ralph B. and Alegria-Leal, Elitzander and Alexander, Kai Y. and Ayala, Angelica C. and Balashova, Nataliya S. and Barber, Rebecca M. and Bassi, Harmanjit and Bennion, Sean P. and Beyder, Miriam and Bhatt, Kush V and Bhoot, Chinmay and Bradshaw, Aaron W. and Brannigan, Tierney G. and Cao, Boyu and Cashell, Yancey Y. and Chai, Timothy and Chan, Alex W. and Chan, Carissa and Chang, Inho and Chang, Jonathan and Chang, Michael T. and Chang, Patrick W. and Chang, Stephen and Chari, Neel and Chassiakos, Alexander J. and Chen, Iris E. and Chen, Vivian K. and Chen, Zheying and Cheng, Marsha R. and Chiang, Mimi and Chiu, Vivian and Choi, Sharon and Chung, Jun Ho and Contreras, Liset and Corona, Edgar and Cruz, Courtney J. and Cruz, Renae L. and Dang, Jefferson M. and Dasari, Suhas P. and De la Fuente, Justin R. O. and Del Rio, Oscar M. A. and Dennis, Emily R. and Dertsakyan, Petros S. and Dey, Ipsita and Distler, Rachel S. and Dong, Zhiqiao and Dorman, Leah C. and Douglass, Mark A. and Ehresman, Allysen B. and Fu, Ivy H. and Fua, Andrea and Full, Sean M. and Ghaffari-Rafi, Arash and Ghani, Asmar Abdul and Giap, Bosco and Gill, Sonia and Gill, Zafar S. and Gills, Nicholas J. and Godavarthi, Sindhuja and Golnazarian, Talin and Goyal, Raghav and Gray, Ricardo and Grunfeld, Alexander M. and Gu, Kelly M. and Gutierrez, Natalia C. and Ha, An N. and Hamid, Iman and Hanson, Ashley and Hao, Celesti and He, Chongbin and He, Mengshi and Hedtke, Joshua P. and Hernandez, Ysrael K. and Hlaing, Hnin and Hobby, Faith A. and Hoi, Karen and Hope, Ashley C. and Hosseinian, Sahra M. and Hsu, Alice and Hsueh, Jennifer and Hu, Eileen and Hu, Spencer S. and Huang, Stephanie and Huang, Wilson and Huynh, Melanie and Javier, Carmen and Jeon, Na Eun and Ji, Sunjong and Johal, Jasmin and John, Amala and Johnson, Lauren and Kadakia, Saurin and Kakade, Namrata and Kamel, Sarah and Kaur, Ravinder and Khatra, Jagteshwar S. and Kho, Jeffrey A. and Kim, Caleb and Kim, Emily Jin-Kyung and Kim, Hee Jong and Kim, Hyun Wook and Kim, Jin Hee and Kim, Seong Ah and Kim, Woo Kyeom and Kit, Brian and La, Cindy and Lai, Jonathan and Lam, Vivian and Nguyen Khoi Le and Lee, Chi Ju and Lee, Dana and Lee, Dong Yeon and Lee, James and Lee, Jason and Lee, Jessica and Lee, Ju-Yeon and Lee, Sharon and Lee, Terrence C. and Lee, Victoria and Li, Amber J. and Li, Jialing and Libro, Alexandra M. and Lien, Irvin C. and Lim, Mia and Lin, Jeffrey M. and Liu, Connie Y. and Liu, Steven C. and Louie, Irene and Lu, Shijia W. and Luo, William Y. and Luu, Tiffany and Madrigal, Josef T. and Mai, Yishan and Miya, Darron I and Mohammadi, Mina and Mohanta, Sayonika and Mokwena, Tebogo and Montoya, Tonatiuh and Mould, Dallas L. and Murata, Mark R. and Muthaiya, Janani and Naicker, Seethim and Neebe, Mallory R. and Ngo, Amy and Ngo, Duy Q. and Ngo, Jamie A. and Nguyen, Anh T. and Nguyen, Huy C. X. and Nguyen, Rina H. and Nguyen, Thao T. T. and Nguyen, Vincent T. and Nishida, Kevin and Oh, Seo-Kyung and Omi, Kristen M. and Onglatco, Mary C. and Almazan, Guadalupe Ortega and Paguntalan, Jahzeel and Panchal, Maharshi and Pang, Stephanie and Parikh, Harin B. and Patel, Purvi D. and Patel, Trisha H. and Petersen, Julia E. and Pham, Steven and Phan-Everson, Tien M. and Pokhriyal, Megha and Popovich, Davis W. and Quaal, Adam T. and Querubin, Karl and Resendiz, Anabel and Riabkova, Nadezhda and Rong, Fred and Salarkia, Sarah and Sama, Nateli and Sang, Elaine and Sanville, David A. and Schoen, Emily R. and Shen, Zhouyang and Siangchin, Ken and Sibal, Gabrielle and Sin, Garuem and Sjarif, Jasmine and Smith, Christopher J. and Soeboer, Annisa N. and Sosa, Cristian and Spitters, Derek and Stender, Bryan and Su, Chloe C. and Summapund, Jenny and Sun, Beatrice J. and Sutanto, Christine and Tan, Jaime S. and Tan, Nguon L. and Tangmatitam, Parich and Trac, Cindy K. and Tran, Conny and Tran, Daniel and Tran, Duy and Tran, Vina and Truong, Patrick A. and Tsai, Brandon L. and Tsai, Pei-Hua and Tsui, C. Kimberly and Uriu, Jackson K. and Venkatesh, Sanan and Vo, Maique and Nhat-Thi Vo and Phuong Vo and Voros, Timothy C. and Wan, Yuan and Wang, Eric and Wang, Jeffrey and Wang, Michael K. and Wang, Yuxuan and Wei, Siman and Wilson, Matthew N. and Wong, Daniel and Wu, Elliott and Xing, Hanning and Xu, Jason P. and Yaftaly, Sahar and Yan, Kimberly and Yang, Evan and Yang, Rebecca and Yao, Tony and Yeo, Patricia and Yip, Vivian and Yogi, Puja and Young, Gloria Chin and Yung, Maggie M. and Zai, Alexander and Zhang, Christine and Zhang, Xiao X. and Zhao, Zijun and Zhou, Raymond and Zhou, Ziqi and Abutouk, Mona and Aguirre, Brian and Ao, Chon and Baranoff, Alexis and Beniwal, Angad and Cai, Zijie and Chan, Ryan and Chien, Kenneth Chang and Chaudhary, Umar and Chin, Patrick and Chowdhury, Praptee and Dalie, Jamlah and Du, Eric Y. and Estrada, Alec and Feng, Erwin and Ghaly, Monica and Graf, Rose and Hernandez, Eduardo and Herrera, Kevin and Ho, Vivien W. and Honeychurch, Kaitlyn and Hou, Yurianna and Huang, Jo M. and Ishii, Momoko and James, Nicholas and Jang, Gah-Eun and Jin, Daphne and Juarez, Jesse and Kesaf, Ayse Elif and Khalsa, Sat Kartar and Kim, Hannah and Kovsky, Jenna and Kuang, Chak Lon and Kumar, Shraddha and Lam, Gloria and Lee, Ceejay and Lee, Grace and Li, Li and Lin, Joshua and Liu, Josephine and Ly, Janice and Ma, Austin and Markovic, Hannah and Medina, Cristian and Mungcal, Jonelle and Naranbaatar, Bilguudei and Patel, Kayla and Petersen, Lauren and Phan, Amanda and Phung, Malcolm and Priasti, Nadiyah and Ruano, Nancy and Salim, Tanveer and Schnell, Kristen and Shah, Paras and Shen, Jinhua and Stutzman, Nathan and Sukhina, Alisa and Tian, Rayna and Vega-Loza, Andrea and Wang, Joyce and Wang, Jun and Watanabe, Rina and Wei, Brandon and Xie, Lillian and Ye, Jessica and Zhao, Jeffrey and Zimmerman, Jill and Bracken, Colton and Capili, Jason and Char, Andrew and Chen, Michel and Huang, Pingdi and Ji, Sena and Kim, Emily and Kim, Kenneth and Ko, Julie and Laput, Sean Louise G. and Law, Sam and Lee, Sang Kuk and Lee, Olivia and Lim, David and Lin, Eric and Marik, Kyle and Mytych, Josh and O'Laughlin, Andie and Pak, Jensen and Park, Claire and Ryu, Ruth and Shinde, Ashwin and Sosa, Manny and Waite, Nick and Williams, Mane and Wong, Richard and Woo, Jocelyn and Woo, Jonathan and Yepuri, Vishaal and Yim, Dorothy and Dan Huynh and Wijiewarnasurya, Dinali and Shapiro, Casey and Levis-Fitzgerald, Marc and Jaworski, Leslie and Lopatto, David and Clark, Ira E. and Johnson, Tracy and Banerjee, Utpal}, doi = {10.1093/g3journal/jkaa028}, journal-iso = {G3-GENES GENOM GENET}, journal = {G3-GENES GENOMES GENETICS}, volume = {11}, unique-id = {32362075}, issn = {2160-1836}, abstract = {Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach.}, keywords = {BLOOD; education; Hematopoiesis; RNAi; Cure}, year = {2021}, eissn = {2160-1836}, orcid-numbers = {Mondal, Bama Charan/0000-0001-9694-7714; Hsu, Alice/0000-0001-6609-2559; Lee, Jason/0000-0003-3487-1042; Markovic, Hannah/0000-0002-9962-773X; Stutzman, Nathan/0000-0002-6722-5656; Lee, Olivia/0000-0002-8854-3709} } @article{MTMT:32000172, title = {Current concepts in granulomatous immune responses}, url = {https://m2.mtmt.hu/api/publication/32000172}, author = {Herbath, M. and Fabry, Z. and Sandor, M.}, doi = {10.1007/s42977-021-00077-1}, journal-iso = {BIOL FUTURA}, journal = {BIOLOGIA FUTURA}, volume = {72}, unique-id = {32000172}, issn = {2676-8615}, abstract = {Persistent irritants that are resistant to innate and cognate immunity induce granulomas. These macrophage-dominated lesions that partially isolate the healthy tissue from the irritant and the irritant induced inflammation. Particles, toxins, autoantigens and infectious agents can induce granulomas. The corresponding lesions can be protective for the host but they can also cause damage and such damage has been associated with the pathology of more than a hundred human diseases. Recently, multiple molecular mechanisms underlying how normal macrophages transform into granuloma-inducing macrophages have been discovered and new information has been gathered, indicating how these lesions are initiated, spread and regulated. In this review, differences between the innate and cognate granuloma pathways are discussed by summarizing how the dendritic cell-T cell axis changes granulomatous immunity. Granuloma lesions are highly dynamic and depend on continuous cell replacement. This feature provides new therapeutic approaches to treat granulomatous diseases. © 2021, Akadémiai Kiadó Zrt.}, keywords = {DENDRITIC CELLS; granuloma; VEGF; Cell traffic}, year = {2021}, eissn = {2676-8607}, pages = {61-68} } @article{MTMT:32587143, title = {Proteasome α6 Subunit Negatively Regulates the JAK/STAT Pathway and Blood Cell Activation in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/32587143}, author = {Järvelä-Stölting, M. and Vesala, L. and Maasdorp, M.K. and Ciantar, J. and Rämet, M. and Valanne, S.}, doi = {10.3389/fimmu.2021.729631}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {12}, unique-id = {32587143}, issn = {1664-3224}, abstract = {JAK/STAT signaling regulates central biological functions such as development, cell differentiation and immune responses. In Drosophila, misregulated JAK/STAT signaling in blood cells (hemocytes) induces their aberrant activation. Using mass spectrometry to analyze proteins associated with a negative regulator of the JAK/STAT pathway, and by performing a genome-wide RNAi screen, we identified several components of the proteasome complex as negative regulators of JAK/STAT signaling in Drosophila. A selected proteasome component, Prosα6, was studied further. In S2 cells, Prosα6 silencing decreased the amount of the known negative regulator of the pathway, ET, leading to enhanced expression of a JAK/STAT pathway reporter gene. Silencing of Prosα6 in vivo resulted in activation of the JAK/STAT pathway, leading to the formation of lamellocytes, a specific hemocyte type indicative of hemocyte activation. This hemocyte phenotype could be partially rescued by simultaneous knockdown of either the Drosophila STAT transcription factor, or MAPKK in the JNK-pathway. Our results suggest a role for the proteasome complex components in the JAK/STAT pathway in Drosophila blood cells both in vitro and in vivo. Copyright © 2021 Järvelä-Stölting, Vesala, Maasdorp, Ciantar, Rämet and Valanne.}, keywords = {RNA Interference; Drosophila melanogaster; Lamellocyte; Hemocyte; Fruit fly; JAK/STAT PATHWAY; Eye Transformer; the proteasome complex}, year = {2021}, eissn = {1664-3224} } @article{MTMT:32362077, title = {Anti-Fibrotic Activity of an Antimicrobial Peptide in a Drosophila Model}, url = {https://m2.mtmt.hu/api/publication/32362077}, author = {Khalili, Dilan and Kalcher, Christina and Baumgartner, Stefan and Theopold, Ulrich}, doi = {10.1159/000516104}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {13}, unique-id = {32362077}, issn = {1662-811X}, abstract = {Fibrotic lesions accompany several pathological conditions, including tumors. We show that expression of a dominant-active form of the Ras oncogene in Drosophila salivary glands (SGs) leads to redistribution of components of the basement membrane (BM) and fibrotic lesions. Similar to several types of mammalian fibrosis, the disturbed BM attracts clot components, including insect transglutaminase and phenoloxidase. SG epithelial cells show reduced apicobasal polarity accompanied by a loss of secretory activity. Both the fibrotic lesions and the reduced cell polarity are alleviated by ectopic expression of the antimicrobial peptide drosomycin (Drs), which also restores the secretory activity of the SGs. In addition to extracellular matrix components, both Drs and F-actin localize to fibrotic lesions.}, keywords = {innate immunity; Fibrosis; ANTIMICROBIAL PEPTIDES; extracellular matrix; insect immunity}, year = {2021}, eissn = {1662-8128}, pages = {376-390}, orcid-numbers = {Baumgartner, Stefan/0000-0001-5320-8321; Theopold, Ulrich/0000-0002-1009-8254} } @article{MTMT:32006156, title = {Macrophages and Their Organ Locations Shape Each Other in Development and Homeostasis – A Drosophila Perspective}, url = {https://m2.mtmt.hu/api/publication/32006156}, author = {Mase, A. and Augsburger, J. and Brückner, K.}, doi = {10.3389/fcell.2021.630272}, journal-iso = {FRONT CELL DEV BIOL}, journal = {FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY}, volume = {9}, unique-id = {32006156}, issn = {2296-634X}, abstract = {Across the animal kingdom, macrophages are known for their functions in innate immunity, but they also play key roles in development and homeostasis. Recent insights from single cell profiling and other approaches in the invertebrate model organism Drosophila melanogaster reveal substantial diversity among Drosophila macrophages (plasmatocytes). Together with vertebrate studies that show genuine expression signatures of macrophages based on their organ microenvironments, it is expected that Drosophila macrophage functional diversity is shaped by their anatomical locations and systemic conditions. In vivo evidence for diverse macrophage functions has already been well established by Drosophila genetics: Drosophila macrophages play key roles in various aspects of development and organogenesis, including embryogenesis and development of the nervous, digestive, and reproductive systems. Macrophages further maintain homeostasis in various organ systems and promote regeneration following organ damage and injury. The interdependence and interplay of tissues and their local macrophage populations in Drosophila have implications for understanding principles of organ development and homeostasis in a wide range of species. © Copyright © 2021 Mase, Augsburger and Brückner.}, keywords = {regeneration; MACROPHAGE; Development; Drosophila melanogaster; Homeostasis; Hemocyte; Plasmatocyte; organ microenvironment}, year = {2021}, eissn = {2296-634X} } @article{MTMT:32006124, title = {Reactive oxygen species: the root cause of nanoparticle-induced toxicity in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/32006124}, author = {Mishra, M. and Panda, M.}, doi = {10.1080/10715762.2021.1914335}, journal-iso = {FREE RADIC RES}, journal = {FREE RADICAL RESEARCH}, volume = {55}, unique-id = {32006124}, issn = {1071-5762}, abstract = {Nanotechnology is a rapidly developing technology in the twenty-first century. Nanomaterials are extensively used in numerous industries including cosmetics, food, medicines, industries, agriculture, etc. Along with its wide application toxicity is also reported from studies of various model organisms including Drosophila. The toxicity reflects cytotoxicity, genotoxicity, and teratogenicity. The current study correlates the toxicity as a consequence of reactive oxygen species (ROS) generated owing to the presence of nanoparticles with the living cell. ROS mainly includes hydroxyl ions, peroxide ions, superoxide anions, singlet oxygen, and hypochlorous acids. An elevated level of ROS can damage the cells by various means. To protect the body from excess ROS, living cells possess a set of antioxidant enzymes which includes peroxidase, glutathione peroxidase, and catalase. If the antioxidant enzymes cannot nullify the elevated ROS level than DNA damage, cell damage, cytotoxicity, apoptosis, and uncontrolled cell regulations occur resulting in abnormal physiological and genotoxic conditions. Herewith, we are reporting various morphological and physiological defects caused after nanoparticle treatment as a function of redox imbalance. © 2021 Informa UK Limited, trading as Taylor & Francis Group.}, keywords = {NANOPARTICLES; Reactive oxygen species; antioxidant enzymes; Redox imbalance; REDOX MECHANISM}, year = {2021}, eissn = {1029-2470}, pages = {671-687} } @article{MTMT:32361805, title = {Drosophila as a Model to Study Cellular Communication Between the Hematopoietic Niche and Blood Progenitors Under Homeostatic Conditions and in Response to an Immune Stress}, url = {https://m2.mtmt.hu/api/publication/32361805}, author = {Morin-Poulard, Ismael and Tian, Yushun and Vanzo, Nathalie and Crozatier, Michele}, doi = {10.3389/fimmu.2021.719349}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {12}, unique-id = {32361805}, issn = {1664-3224}, abstract = {In adult mammals, blood cells are formed from hematopoietic stem progenitor cells, which are controlled by a complex cellular microenvironment called "niche". Drosophila melanogaster is a powerful model organism to decipher the mechanisms controlling hematopoiesis, due both to its limited number of blood cell lineages and to the conservation of genes and signaling pathways throughout bilaterian evolution. Insect blood cells or hemocytes are similar to the mammalian myeloid lineage that ensures innate immunity functions. Like in vertebrates, two waves of hematopoiesis occur in Drosophila. The first wave takes place during embryogenesis. The second wave occurs at larval stages, where two distinct hematopoietic sites are identified: subcuticular hematopoietic pockets and a specialized hematopoietic organ called the lymph gland. In both sites, hematopoiesis is regulated by distinct niches. In hematopoietic pockets, sensory neurons of the peripheral nervous system provide a microenvironment that promotes embryonic hemocyte expansion and differentiation. In the lymph gland blood cells are produced from hematopoietic progenitors. A small cluster of cells called Posterior Signaling Centre (PSC) and the vascular system, along which the lymph gland develops, act collectively as a niche, under homeostatic conditions, to control the balance between maintenance and differentiation of lymph gland progenitors. In response to an immune stress such as wasp parasitism, lymph gland hematopoiesis is drastically modified and shifts towards emergency hematopoiesis, leading to increased progenitor proliferation and their differentiation into lamellocyte, a specific blood cell type which will neutralize the parasite. The PSC is essential to control this emergency response. In this review, we summarize Drosophila cellular and molecular mechanisms involved in the communication between the niche and hematopoietic progenitors, both under homeostatic and stress conditions. Finally, we discuss similarities between mechanisms by which niches regulate hematopoietic stem/progenitor cells in Drosophila and mammals.}, keywords = {DROSOPHILA; niche; Hematopoiesis; lymph gland; immune stress}, year = {2021}, eissn = {1664-3224} } @article{MTMT:34589425, title = {Extracellular matrix protein N-glycosylation mediates immune self-tolerance in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/34589425}, author = {Mortimer, Nathan T. and Fischer, Mary L. and Waring, Ashley L. and Pooja, K. R. and Kacsoh, Balint Z. and Brantley, Susanna E. and Keebaugh, Erin S. and Hill, Joshua and Lark, Chris and Martin, Julia and Bains, Pravleen and Lee, Jonathan and Vrailas-Mortimer, Alysia D. and Schlenke, Todd A.}, doi = {10.1073/pnas.2017460118|1of12}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {118}, unique-id = {34589425}, issn = {0027-8424}, abstract = {In order to respond to infection, hosts must distinguish pathogens from their own tissues. This allows for the precise targeting of immune responses against pathogens and also ensures self-tolerance, the ability of the host to protect self tissues from immune damage. One way to maintain self-tolerance is to evolve a self signal and suppress any immune response directed at tissues that carry this signal. Here, we characterize the Drosophila tuSz1 mutant strain, which mounts an aberrant immune response against its own fat body. We demonstrate that this autoimmunity is the result of two mutations: 1) a mutation in the GCS1 gene that disrupts N-glycosylation of extracellular matrix proteins covering the fat body, and 2) a mutation in the Drosophila Janus Kinase ortholog that causes precocious activation of hemocytes. Our data indicate that N-glycans attached to extracellular matrix proteins serve as a self signal and that activated hemocytes attack tissues lacking this signal. The simplicity of this invertebrate self-recognition system and the ubiquity of its constituent parts suggests it may have functional homologs across animals.}, keywords = {X-CHROMOSOME; innate immunity; innate immunity; Autoimmunity; BLOOD-CELLS; SELF-TOLERANCE; self-recognition; Hematopoietic progenitors; MISSING-SELF; HEMOCYTE LINEAGES; MELANOTIC TUMOR-FORMATION; TEMPERATURE-SENSITIVE MUTATIONS; ENCAPSULATION RESPONSE}, year = {2021}, eissn = {1091-6490}, orcid-numbers = {Mortimer, Nathan T./0000-0003-3787-9445; Waring, Ashley L./0000-0002-4763-2502; Kacsoh, Balint Z./0000-0001-9171-0611; Martin, Julia/0000-0001-9260-5784; Vrailas-Mortimer, Alysia D./0000-0001-5927-096X} } @article{MTMT:32876736, title = {Extracellular matrix protein N-glycosylation mediates immune self-tolerance in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/32876736}, author = {Mortimer, N.T. and Fischer, M.L. and Waring, A.L. and Pooja, K.R. and Kacsoh, B.Z. and Brantley, S.E. and Keebaugh, E.S. and Hill, J. and Lark, C. and Martin, J. and Bains, P. and Lee, J. and Vrailas-Mortimer, A.D. and Schlenke, T.A.}, doi = {10.1073/pnas.2017460118}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {118}, unique-id = {32876736}, issn = {0027-8424}, abstract = {In order to respond to infection, hosts must distinguish pathogens from their own tissues. This allows for the precise targeting of immune responses against pathogens and also ensures self-tolerance, the ability of the host to protect self tissues from immune damage. One way to maintain self-tolerance is to evolve a self signal and suppress any immune response directed at tissues that carry this signal. Here, we characterize the Drosophila tuSz1 mutant strain, which mounts an aberrant immune response against its own fat body. We demonstrate that this autoimmunity is the result of two mutations: 1) a mutation in the GCS1 gene that disrupts N-glycosylation of extracellular matrix proteins covering the fat body, and 2) a mutation in the Drosophila Janus Kinase ortholog that causes precocious activation of hemocytes. Our data indicate that N-glycans attached to extracellular matrix proteins serve as a self signal and that activated hemocytes attack tissues lacking this signal. The simplicity of this invertebrate self-recognition system and the ubiquity of its constituent parts suggests it may have functional homologs across animals. © 2021 National Academy of Sciences. All rights reserved.}, keywords = {Animals; Adult; Female; Male; GENE; MUTATION; MUTATION; metabolism; GENETICS; ARTICLE; HEMOCYTES; immunology; animal; growth, development and aging; innate immunity; gene mutation; controlled study; nonhuman; Drosophila melanogaster; Drosophila melanogaster; immunological tolerance; immunological tolerance; cell activation; immune response; blood cell; glycosylation; glycosylation; Autoimmunity; Autoimmunity; Extracellular Matrix Proteins; Immune Tolerance; scleroprotein; scleroprotein; Janus kinase; Janus kinase; Janus Kinases; protein glycosylation; Self Tolerance; Drosophila Proteins; Drosophila protein; FAT PAD; SELF-TOLERANCE; self-recognition; protein N-glycosylation; GCS1 gene}, year = {2021}, eissn = {1091-6490} } @article{MTMT:32601957, title = {Dietary cadmium (Cd) reduces hemocyte level by induction of apoptosis in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/32601957}, author = {Nanda, K.P. and Firdaus, H.}, doi = {10.1016/j.cbpc.2021.109188}, journal-iso = {COMP BIOCHEM PHYS C}, journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY AND PHARMACOLOGY}, volume = {250}, unique-id = {32601957}, issn = {1532-0456}, abstract = {Drosophila melanogaster larvae ensure continuous proliferation and differentiation of hemocytes to maintain a fixed range of different blood cell types during its various stages of development. Variation in this number is often an indicator of animal well-being, its genotype or an effect of environmental perturbation, including exposure to heavy metals. The present study investigates the effect of Cd on larval hemocytes. Embryos were allowed to grow in metal media till third instar larvae and finally circulating hemocyte were collected. The number of major hemocytes, plasmatocytes and crystal cells was determined to be lowered in Cd exposed animals. Our results also showed modulation of antioxidant biology of Cd exposed hemocytes by changing the major antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) activity, and decreasing reduced glutathione (GSH) levels in hemocytes suspended in the hemolymph. Acridine orange (AO) staining further revealed induction of apoptosis in hemocytes of metal treated larvae. Our results suggest a negative impact of Cd exposure on the hemocytes of the Drosophila larvae culminating in their lowered count by induction of apoptosis. © 2021 Elsevier Inc.}, keywords = {APOPTOSIS; APOPTOSIS; ARTICLE; DROSOPHILA; HEMOCYTES; controlled study; GLUTATHIONE; nonhuman; animal cell; enzyme activity; cadmium; cadmium; Drosophila melanogaster; embryo; hydrogen peroxide; Catalase; crystal structure; cellular immunity; blood cell; Superoxide dismutase; plasma cell; antioxidant enzymes; dietary intake; Acridine Orange; host pathogen interaction; mitochondrial membrane potential; Hemolymph; fluorescence intensity; Oxidative stress}, year = {2021}, eissn = {1878-1659} } @article{MTMT:32601905, title = {Intermediate progenitor cells provide a transition between hematopoietic progenitors and their differentiated descendants}, url = {https://m2.mtmt.hu/api/publication/32601905}, author = {Spratford, C.M. and Goins, L.M. and Chi, F. and Girard, J.R. and Macias, S.N. and Ho, V.W. and Banerjee, U.}, doi = {10.1242/dev.200216}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {148}, unique-id = {32601905}, issn = {0950-1991}, abstract = {Genetic and genomic analysis in Drosophila suggests that hematopoietic progenitors likely transition into terminal fates via intermediate progenitors (IPs) with some characteristics of either, but perhaps maintaining IP-specific markers. In the past, IPs have not been directly visualized and investigated owing to lack of appropriate genetic tools. Here, we report a Split GAL4 construct, CHIZ-GAL4, that identifies IPs as cells physically juxtaposed between true progenitors and differentiating hemocytes. IPs are a distinct cell type with a unique cell-cycle profile and they remain multipotent for all blood cell fates. In addition, through their dynamic control of the Notch ligand Serrate, IPs specify the fate of direct neighbors. The Ras pathway controls the number of IP cells and promotes their transition into differentiating cells. This study suggests that it would be useful to characterize such intermediate populations of cells in mammalian hematopoietic systems. © 2021. Published by The Company of Biologists Ltd}, keywords = {DROSOPHILA; Hematopoiesis; Blood cell development; crystal cells; Intermediate progenitor; Split GAL4}, year = {2021}, eissn = {1477-9129} } @article{MTMT:32362073, title = {Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing}, url = {https://m2.mtmt.hu/api/publication/32362073}, author = {Tattikota, Sudhir Gopal and Perrimon, Norbert}, doi = {10.21769/BioProtoc.4127}, journal-iso = {BIO-PROTOCOL}, journal = {BIO-PROTOCOL}, volume = {11}, unique-id = {32362073}, issn = {2331-8325}, abstract = {Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain high-quality single cells in suspension. Recently, we used this approach to characterize Drosophila blood cells (hemocytes). Here, we provide a detailed protocol for obtaining single hemocytes in suspension, which can be used for microfluidics-based scRNA-seq platforms. This protocol involves the simple bleeding of third instar larvae and the subsequent purification of the hemolymph using either Optiprep-based gradient centrifugation or traditional centrifugation methods to obtain single hemocytes of high quality for scRNA-seq. Importantly, this method for single-hemocyte preparation is straightforward and reproducible, with negligible issues associated with cell viability as the entire procedure involves no enzymatic dissociation.[GRAPHICS].}, keywords = {DROSOPHILA; HEMOCYTES; Blood cells; CIRCULATION; Hemolymph; ScRNA-seq; Sessile}, year = {2021}, orcid-numbers = {Tattikota, Sudhir Gopal/0000-0003-0318-5533} } @article{MTMT:31868552, title = {Immune cell production is targeted by parasitoid wasp virulence in a drosophila–parasitoid wasp interaction}, url = {https://m2.mtmt.hu/api/publication/31868552}, author = {Trainor, J.E. and Pooja, K.R. and Mortimer, N.T.}, doi = {10.3390/pathogens10010049}, journal-iso = {PATHOGENS}, journal = {PATHOGENS}, volume = {10}, unique-id = {31868552}, abstract = {The interactions between Drosophila melanogaster and the parasitoid wasps that infect Drosophila species provide an important model for understanding host–parasite relationships. Following parasitoid infection, D. melanogaster larvae mount a response in which immune cells (hemocytes) form a capsule around the wasp egg, which then melanizes, leading to death of the parasitoid. Previous studies have found that host hemocyte load; the number of hemocytes available for the encapsulation response; and the production of lamellocytes, an infection induced hemocyte type, are major determinants of host resistance. Parasitoids have evolved various virulence mechanisms to overcome the immune response of the D. melanogaster host, including both active immune suppression by venom proteins and passive immune evasive mechanisms. We identified a previously undescribed parasitoid species, Asobara sp. AsDen, which utilizes an active virulence mechanism to infect D. melanogaster hosts. Asobara sp. AsDen infection inhibits host hemocyte expression of msn, a member of the JNK signaling pathway, which plays a role in lamellocyte production. Asobara sp. AsDen infection restricts the production of lamellocytes as assayed by hemocyte cell morphology and altered msn expression. Our findings suggest that Asobara sp. AsDen infection alters host signaling to suppress immunity. © 2021 by the authors. Li-censee MDPI, Basel, Switzerland.}, keywords = {Female; ARTICLE; VENOM; signal transduction; PHYLOGENY; DROSOPHILA; DROSOPHILA; controlled study; nonhuman; sequence analysis; Drosophila melanogaster; immunocompetent cell; encapsulation; immune response; blood cell; principal component analysis; cell structure; immunosuppressive treatment; bacterial virulence; passive immunization; DNA extraction; Lamellocyte; Parasitoid; Sanger sequencing; Parasitoid wasp; Immune cell; Virulence strategy; hemolymphatic system}, year = {2021}, eissn = {2076-0817}, pages = {1-16} } @article{MTMT:32358968, title = {Proteomics of purified lamellocytes from Drosophila melanogaster HopTum-l identifies new membrane proteins and networks involved in their functions}, url = {https://m2.mtmt.hu/api/publication/32358968}, author = {Wan, Bin and Belghazi, Maya and Lemauf, Severine and Poirie, Marylene and Gatti, Jean-Luc}, doi = {10.1016/j.ibmb.2021.103584}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {134}, unique-id = {32358968}, issn = {0965-1748}, abstract = {In healthy Drosophila melanogaster larvae, plasmatocytes and crystal cells account for 95% and 5% of the hemocytes, respectively. A third type of hemocytes, lamellocytes, are rare, but their number increases after oviposition by parasitoid wasps. The lamellocytes form successive layers around the parasitoid egg, leading to its encapsulation and melanization, and finally the death of this intruder. However, the total number of lamellocytes per larva remains quite low even after parasitoid infestation, making direct biochemical studies difficult. Here, we used the HopTum-l mutant strain that constitutively produces large numbers of lamellocytes to set up a purification method and analyzed their major proteins by 2D gel electrophoresis and their plasma membrane surface proteins by 1D SDS-PAGE after affinity purification. Mass spectrometry identified 430 proteins from 2D spots and 344 affinity-purified proteins from 1D bands, for a total of 639 unique proteins. Known lamellocyte markers such as PPO3 and the myospheroid integrin were among the components identified with specific chaperone proteins. Affinity purification detected other integrins, as well as a wide range of integrin-associated proteins involved in the formation and function of cell-cell junctions. Overall, the newly identified proteins indicate that these cells are highly adapted to the encapsulation process (recognition, motility, adhesion, signaling), but may also have several other physiological functions (such as secretion and internalization of vesicles) under different signaling pathways. These results provide the basis for further in vivo and in vitro studies of lamellocytes, including the development of new markers to identify coexisting populations and their respective origins and functions in Drosophila immunity.}, keywords = {Drosophila melanogaster; protein purification; proteomics; lamellocytes; Hemocytes purification; Q-orbitrap spectrometry}, year = {2021}, eissn = {1879-0240}, orcid-numbers = {Belghazi, Maya/0000-0002-3600-4754} } @article{MTMT:31596046, title = {Cellular and humoral immune interactions between Drosophila and its parasitoids}, url = {https://m2.mtmt.hu/api/publication/31596046}, author = {Yang, L. and Qiu, L.-M. and Fang, Q. and Stanley, D.W. and Ye, G.-Y.}, doi = {10.1111/1744-7917.12863}, journal-iso = {INSECT SCI}, journal = {INSECT SCIENCE}, volume = {28}, unique-id = {31596046}, issn = {1672-9609}, abstract = {The immune interactions occurring between parasitoids and their host insects, especially in Drosophila–wasp models, have long been the research focus of insect immunology and parasitology. Parasitoid infestation in Drosophila is counteracted by its multiple natural immune defense systems, which include cellular and humoral immunity. Occurring in the hemocoel, cellular immune responses involve the proliferation, differentiation, migration and spreading of host hemocytes and parasitoid encapsulation by them. Contrastingly, humoral immune responses rely more heavily on melanization and on the Toll, Imd and Jak/Stat immune pathways associated with antimicrobial peptides along with stress factors. On the wasps’ side, successful development is achieved by introducing various virulence factors to counteract immune responses of Drosophila. Some or all of these factors manipulate the host's immunity for successful parasitism. Here we review current knowledge of the cellular and humoral immune interactions between Drosophila and its parasitoids, focusing on the defense mechanisms used by Drosophila and the strategies evolved by parasitic wasps to outwit it. © 2020 Institute of Zoology, Chinese Academy of Sciences}, keywords = {VENOM; DROSOPHILA; Immunity; Parasitoid; VIRUS-LIKE PARTICLES}, year = {2021}, eissn = {1744-7917}, pages = {1208-1227} } @article{MTMT:31468414, title = {The Leukemic Fly: Promises and Challenges}, url = {https://m2.mtmt.hu/api/publication/31468414}, author = {Al Outa, Amani and Abubaker, Dana and Madi, Joelle and Nasr, Rihab and Shirinian, Margret}, doi = {10.3390/cells9071737}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {9}, unique-id = {31468414}, abstract = {Leukemia involves different types of blood cancers, which lead to significant mortality and morbidity. Murine models of leukemia have been instrumental in understanding the biology of the disease and identifying therapeutics. However, such models are time consuming and expensive in high throughput genetic and drug screening.Drosophilamelanogasterhas emerged as an invaluable in vivo model for studying different diseases, including cancer. Fruit flies possess several hematopoietic processes and compartments that are in close resemblance to their mammalian counterparts. A number of studies succeeded in characterizing the fly's response upon the expression of human leukemogenic proteins in hematopoietic and non-hematopoietic tissues. Moreover, some of these studies showed that these models are amenable to genetic screening. However, none were reported to be tested for drug screening. In this review, we describe theDrosophilahematopoietic system, briefly focusing on leukemic diseases in which fruit flies have been used. We discuss myeloid and lymphoid leukemia fruit fly models and we further highlight their roles for future therapeutic screening. In conclusion, fruit fly leukemia models constitute an interesting area which could speed up the process of integrating new therapeutics when complemented with mammalian models.}, keywords = {LEUKEMIA; Drosophila melanogaster; drug screening; Fruit fly; Blood cancer}, year = {2020}, eissn = {2073-4409}, orcid-numbers = {Nasr, Rihab/0000-0003-1166-4999} } @article{MTMT:31468836, title = {Maternal Priming of Offspring Immune System in Drosophila}, url = {https://m2.mtmt.hu/api/publication/31468836}, author = {Bozler, Julianna and Kacsoh, Balint Z. and Bosco, Giovanni}, doi = {10.1534/g3.119.400852}, journal-iso = {G3-GENES GENOM GENET}, journal = {G3-GENES GENOMES GENETICS}, volume = {10}, unique-id = {31468836}, issn = {2160-1836}, abstract = {Immune priming occurs when a past infection experience leads to a more effective immune response upon a secondary exposure to the infection or pathogen. In some instances, parents are able to transmit immune priming to their offspring, creating a subsequent generation with a superior immune capability, through processes that are not yet fully understood. Using a parasitoid wasp, which infects larval stages of Drosophila melanogaster, we describe an example of an intergenerational inheritance of immune priming. This phenomenon is anticipatory in nature and does not rely on parental infection, but rather, when adult fruit flies are cohabitated with a parasitic wasp, they produce offspring that are more capable of mounting a successful immune response against a parasitic macro-infection. This increase in offspring survival correlates with a more rapid induction of lamellocytes, a specialized immune cell. RNA-sequencing of the female germline identifies several differentially expressed genes following wasp exposure, including the peptiodoglycan recognition protein-LB (PGRP-LB). We find that genetic manipulation of maternal PGRP-LB identifies this gene as a key element in this intergenerational phenotype.}, keywords = {Immunity; immune priming; intergenerational; transgenerational; leptopilina heterotoma; leptopilina victoriae; PGRP-LB}, year = {2020}, eissn = {2160-1836}, pages = {165-175}, orcid-numbers = {Bosco, Giovanni/0000-0002-8889-9895} } @article{MTMT:31460653, title = {Temporal specificity and heterogeneity ofDrosophilaimmune cells}, url = {https://m2.mtmt.hu/api/publication/31460653}, author = {Cattenoz, Pierre B. and Sakr, Rosy and Pavlidaki, Alexia and Delaporte, Claude and Riba, Andrea and Molina, Nacho and Hariharan, Nivedita and Mukherjee, Tina and Giangrande, Angela}, doi = {10.15252/embj.2020104486}, journal-iso = {EMBO J}, journal = {EMBO JOURNAL}, volume = {39}, unique-id = {31460653}, issn = {0261-4189}, abstract = {Immune cells provide defense against non-self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity ofDrosophilaimmune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single-cell RNA sequencing, we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct processes, e.g., proliferation, phagocytosis, metabolic homeostasis, and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation, which triggers the differentiation of a novel hemocyte type, the lamellocyte. This first molecular atlas of hemocytes provides insights and paves the way to study the biology of theDrosophilaimmune cells in physiological and pathological conditions.}, keywords = {Drosophila melanogaster; immune cells; Single-cell RNA-seq; wasp infestation}, year = {2020}, eissn = {1460-2075} } @article{MTMT:30819399, title = {Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid Zaprionus indianus}, url = {https://m2.mtmt.hu/api/publication/30819399}, author = {Cinege, Gyöngyi Ilona and Lerner, Zita and Magyar, Lilla Brigitta and Soós, Bálint and Tóth, Renáta and Kristó, Ildikó and Vilmos, Péter and Juhász, Gábor and Kovács, Attila Lajos and Hegedűs, Zoltán and Sensen, Christoph W. and Kurucz, Judit Éva and Andó, István}, doi = {10.1159/000502646}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {12}, unique-id = {30819399}, issn = {1662-811X}, year = {2020}, eissn = {1662-8128}, pages = {257-272}, orcid-numbers = {Juhász, Gábor/0000-0001-8548-8874; Andó, István/0000-0002-4648-9396} } @article{MTMT:31686151, title = {Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments}, url = {https://m2.mtmt.hu/api/publication/31686151}, author = {Csordás, Gábor and Grawe, Ferdinand and Uhlirova, Mirka}, doi = {10.7554/eLife.57297}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {9}, unique-id = {31686151}, issn = {2050-084X}, abstract = {Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation. The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood. Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments. We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment. We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association. These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila}, year = {2020}, eissn = {2050-084X}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839} } @article{MTMT:31468869, title = {A RhoGAP venom protein from Microplitis mediator suppresses the cellular response of its host Helicoverpa armigera}, url = {https://m2.mtmt.hu/api/publication/31468869}, author = {Du, Jie and Lin, Zhe and Volovych, Olga and Lu, Zhiqiang and Zou, Zhen}, doi = {10.1016/j.dci.2020.103675}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {108}, unique-id = {31468869}, issn = {0145-305X}, abstract = {Female parasitoid wasps normally inject virulence factors together with eggs into their host to counter host immunity defenses. A newly identified RhoGAP protein in the venom of Microplitis mediator compromises the cellular immunity of its host, Helicoverpa armigera. RhoGAP1 proteins entered H. armigera hemocytes, and the host cellular cytoskeleton was disrupted. Depletion of MmGAP1 by injection of dsRNA or antibody increased the wasp egg encapsulation rate. An immunoprecipitation assay of overexpressed MmGAP1 protein in a Helicoverpa cell line showed that MmGAP1 interacts with many cellular cytoskeleton associated proteins as well as Rho GTPases. A yeast two-hybrid and a pull-down assay demonstrated that MmGAP1 interacts with H. armigera RhoA and Cdc42. These results show that the RhoGAP protein in M. mediator can destroy the H. armigera hemocyte cellular cytoskeleton, restrain host cellular immune defense, and increase the probability of successful parasitism.}, keywords = {VENOM; Virulence; cellular immunity; Rho GTPase; RhoGAP}, year = {2020}, eissn = {1879-0089} } @article{MTMT:31470099, title = {Drosophilamelanogaster Responses against Entomopathogenic Nematodes: Focus on Hemolymph Clots}, url = {https://m2.mtmt.hu/api/publication/31470099}, author = {Dziedziech, Alexis and Shivankar, Sai and Theopold, Ulrich}, doi = {10.3390/insects11010062}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {11}, unique-id = {31470099}, abstract = {Several insect innate immune mechanisms are activated in response to infection by entomopathogenic nematodes (EPNs). In this review, we focus on the coagulation of hemolymph, which acts to stop bleeding after injury and prevent access of pathogens to the body cavity. After providing a general overview of invertebrate coagulation systems, we discuss recent findings in Drosophila melanogaster which demonstrate that clots protect against EPN infections. Detailed analysis at the cellular level provided insight into the kinetics of the secretion of Drosophila coagulation factors, including non-classical modes of secretion. Roughly, clot formation can be divided into a primary phase in which crosslinking of clot components depends on the activity of Drosophila transglutaminase and a secondary, phenoloxidase (PO)-dependent phase, characterized by further hardening and melanization of the clot matrix. These two phases appear to play distinct roles in two commonly used EPN infection models, namely Heterorhabditis bacteriophora and Steinernema carpocapsae. Finally, we discuss the implications of the coevolution between parasites such as EPNs and their hosts for the dynamics of coagulation factor evolution.}, keywords = {TRANSGLUTAMINASE; COAGULATION; SECRETION; HEMOCYTES; innate immunity; Nematodes; clotting; phenoloxidase; insect immunity}, year = {2020}, eissn = {2075-4450}, orcid-numbers = {Dziedziech, Alexis/0000-0001-7647-7639; Theopold, Ulrich/0000-0002-1009-8254} } @article{MTMT:31868548, title = {Drosophila metamorphosis involves hemocyte mediated macroendocytosis and efferocytosis}, url = {https://m2.mtmt.hu/api/publication/31868548}, author = {Ghosh, S. and Ghosh, S. and Mandal, L.}, doi = {10.1387/ijdb.190215lm}, journal-iso = {INT J DEV BIOL}, journal = {INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY}, volume = {64}, unique-id = {31868548}, issn = {0214-6282}, abstract = {Drosophila hemocytes are majorly associated with immune responses, but they also undertake several non-immune functions that are crucial during various stages of development. The activity and behaviour of hemocytes are least documented during the metamorphic phase of fly development. Here we describe the activity, form and behaviour of the most abundant type of hemocyte in Drosophila melanogaster, the “plasmatocyte,” throughout pupal development. Our study reveals different forms of plasmatocytes laden with varying degrees of histolyzing debris (muscle and fat) which extend beyond the size of the cell itself, highlighting the phagocytic capacity of these plasmatocytes. Interestingly, the engulfment of apoptotic debris by plasmatocytes is an actin-dependent process, and by the end of metamorphosis, clearance is achieved. The uptake of apoptotic debris consisting of muscles and lipids by the plasmatocytes provides us a model that can be employed to dissect out the relevant components of macroendocytosis and lipid-loaded phagocytosis.This understanding, by itself, is crucial for addressing the emerging role of phagocytes in physiology and pathophysiology. © 2020 UPV/EHU Press.}, keywords = {PHAGOCYTOSIS; MUSCLE; ARTICLE; ENDOCYTOSIS; DROSOPHILA; priority journal; controlled study; nonhuman; animal cell; ACTIN; MOLECULAR RECOGNITION; Drosophila melanogaster; Cell Size; lipid; enzyme degradation; blood cell; plasma cell; bioaccumulation; cell transport; cell activity; metamorphosis; lysozyme; Hemocyte; Pupa; Pupa; Plasmatocyte; Efferocytosis; Efferocytosis; macroendocytosis}, year = {2020}, eissn = {1696-3547}, pages = {309-319} } @article{MTMT:31149641, title = {The insect circulatory system: Structure, function, and evolution}, url = {https://m2.mtmt.hu/api/publication/31149641}, author = {Hillyer, J.F. and Pass, G.}, doi = {10.1146/annurev-ento-011019-025003}, journal-iso = {ANNU REV ENTOMOL}, journal = {ANNUAL REVIEW OF ENTOMOLOGY}, volume = {65}, unique-id = {31149641}, issn = {0066-4170}, abstract = {Although the insect circulatory system is involved in a multitude of vital physiological processes, it has gone grossly understudied. This review highlights this critical physiological system by detailing the structure and function of the circulatory organs, including the dorsal heart and the accessory pulsatile organs that supply hemolymph to the appendages. It also emphasizes how the circulatory system develops and ages and how, by means of reflex bleeding and functional integration with the immune system, it supports mechanisms for defense against predators and microbial invaders, respectively. Beyond that, this review details evolutionary trends and novelties associated with this system, as well as the ways in which this system also plays critical roles in thermoregulation and tracheal ventilation in high-performance fliers. Finally, this review highlights how novel discoveries could be harnessed for the control of vector-borne diseases and for translational medicine, and it details principal knowledge gaps that necessitate further investigation. Copyright © 2020 by Annual Reviews. All rights reserved.}, keywords = {PRESSURE; HEART; Defense; Hemolymph; dorsal vessel; hemocoel}, year = {2020}, eissn = {1545-4487}, pages = {121-143} } @article{MTMT:31470092, title = {The Role of Lozenge in Drosophila Hematopoiesis}, url = {https://m2.mtmt.hu/api/publication/31470092}, author = {Koranteng, Ferdinand and Cha, Nuri and Shin, Mingyu and Shim, Jiwon}, doi = {10.14348/molcells.2019.0249}, journal-iso = {MOL CELLS}, journal = {MOLECULES AND CELLS}, volume = {43}, unique-id = {31470092}, issn = {1016-8478}, abstract = {Drosophila hematopoiesis is comparable to mammalian differentiation of myeloid lineages, and therefore, has been a useful model organism in illustrating the molecular and genetic basis for hematopoiesis. Multiple novel regulators and signals have been uncovered using the tools of Drosophila genetics. A Runt domain protein, lozenge, is one of the first players recognized and closely studied in the hematopoietic lineage specification. Here, we explore the role of lozenge in determination of prohemocytes into a special class of hemocyte, namely the crystal cell, and discuss molecules and signals controlling the lozenge function and its implication in immunity and stress response. Given the highly conserved nature of Runt domain in both invertebrates and vertebrates, studies in Drosophila will enlighten our perspectives on Runx-mediated development and pathologies.}, keywords = {Drosophila melanogaster; Hematopoiesis; lozenge; melanization; crystal cells; lymph gland; prophenoloxidase; RUNX}, year = {2020}, eissn = {0219-1032}, pages = {114-120}, orcid-numbers = {Koranteng, Ferdinand/0000-0002-0545-2423; Shim, Jiwon/0000-0003-2409-1130} } @article{MTMT:31686813, title = {Regulation ofDrosophilaHematopoiesis in Lymph Gland: From a Developmental Signaling Point of View}, url = {https://m2.mtmt.hu/api/publication/31686813}, author = {Lan, Wenwen and Liu, Sumin and Zhao, Long and Su, Ying}, doi = {10.3390/ijms21155246}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {21}, unique-id = {31686813}, issn = {1661-6596}, abstract = {TheDrosophilahematopoietic system is becoming increasingly attractive for its simple blood cell lineage and its developmental and functional parallels with the vertebrate system. As the dedicated organ forDrosophilalarval hematopoiesis, the lymph gland harbors both multipotent stem-like progenitor cells and differentiated blood cells. The balance between progenitor maintenance and differentiation in the lymph gland must be precisely and tightly controlled. Multiple developmental signaling pathways, such as Notch, Hedgehog, and Wnt/Wingless, have been demonstrated to regulate the hematopoietic processes in the lymph gland. Focusing on blood cell maintenance and differentiation, this article summarizes the functions of several classic developmental signaling pathways for lymph gland growth and patterning, highlighting the important roles of developmental signaling during lymph gland development as well asDrosophilalarval hematopoiesis.}, keywords = {Drosophila melanogaster; Hematopoiesis; lymph gland; developmental signaling}, year = {2020}, eissn = {1422-0067}, orcid-numbers = {Su, Ying/0000-0002-8466-0043} } @article{MTMT:31470100, title = {Physiological evidence of integrin-antibody reactive proteins influencing the innate cellular immune responses of larval Galleria mellonella hemocytes}, url = {https://m2.mtmt.hu/api/publication/31470100}, author = {Lapointe, Jason F. and McCarthy, Connor D. and Dunphy, Gary B. and Mandato, Craig A.}, doi = {10.1111/1744-7917.12646}, journal-iso = {INSECT SCI}, journal = {INSECT SCIENCE}, volume = {27}, unique-id = {31470100}, issn = {1672-9609}, abstract = {Larval Galleria mellonella (L.) hemocytes form microaggregates in response to stimulation by Gram-positive bacteria. Hemocyte adhesion to foreign materials is mediated by the cAMP/ protein kinase A pathway and the beta-subunit of cholera toxin using a cAMP-independent mechanism. Cholera toxin-induced microaggregation was inhibited by the integrin inhibitory RGDS peptide, implying integrins may be part of the mechanism. Based on the types of mammalian integrin-antibody reactive proteins affecting hemocyte adhesion and bacterial-induced responses alpha(5), alpha(v), beta(1), and beta(3) subunits occurred on both granular cell and plasmatocyte hemocyte subtypes. A fluorescent band representing the binding of rabbit alpha(5)-integrin subunit antibodies occurred between adhering heterotypic hemocytes. The frequency of the bands was increased by cholera toxin. The alpha(5) and beta(1) rabbit integrin subunit antibodies inhibited removal of Bacillus subtilis (Cohn) from the hemolymph in vivo. A alpha(5) beta(1)-specific synthetic peptide blocker similarly diminished hemocyte function whereas the alpha(v) beta(3)-specific inhibitory peptide and the corresponding integrin subunit antibodies did not influence nonself hemocyte activities. Western blots revealed several proteins reacting with a given integrin-antibody subtype. Thus integrin-antibody reactive proteins (which may include integrins) with possible alpha(5) and beta(1) epitopes modulate immediate hemocyte function. Confocal microscopy established plasmatocyte adhesion to and rosetting over substrata followed by granular cell microaggregate adhesion to plasmatocytes during early stage nodulation.}, keywords = {Hemocyte response; integrin-antibody reactive protein}, year = {2020}, eissn = {1744-7917}, pages = {239-255} } @article{MTMT:31470094, title = {Vegetative development and host immune interaction of Ophiocordyceps sinensis within the hemocoel of the ghost moth larva, Thitarodes xiaojinensis}, url = {https://m2.mtmt.hu/api/publication/31470094}, author = {Li, Miaomiao and Meng, Qian and Zhang, Huan and Ni, Ruoyao and Zhou, Guiling and Zhao, Yanni and Wu, Peipei and Shu, Ruihao and Qin, Qilian and Zhang, Jihong}, doi = {10.1016/j.jip.2020.107331}, journal-iso = {J INVERTEBR PATHOL}, journal = {JOURNAL OF INVERTEBRATE PATHOLOGY}, volume = {170}, unique-id = {31470094}, issn = {0022-2011}, abstract = {Ophiocordyceps sinensis is an entomopathogenic fungus that infects ghost moth larva, forming the most valuable and rare traditional Chinese medicine, Chinese cordyceps. Our knowledge of the basic morphology and developmental biology of Chinese cordyceps is limited. In this study, morphological and ultrastructural observations of O. sinensis development in the hemocoel of Thitarodes xiaojinensis were obtained by multiple light and electron microscopy techniques, and the host immune reaction activities were determined. Our results indicated that fungal cells in the host hemocoel underwent morphotype transformations from blastospores to prehyphae to hyphae in sequence. The fusiform yeast-like blastospores were the initial cell type present in the host hemocoel and remained for 5 months or more; the encapsulation reaction and phenoloxidase activity of T. xiaojinensis hemolymph were inhibited during this period. When larvae entered the last instar, the blastospores switched to prehyphae and expanded throughout the host tissues, and then hyphae germinated from the prehyphae and mycelia formed, which finally led to host death. Considering the distinct differences between blastospores and hyphae, we identified prehyphae, which play important roles in fungal expansion, hyphae germination, and fusion formation among filaments. Notably, the elongation of prehyphae was strongly presumed to occur through fission but without separation of the two sister cells, in contrast to blastospore budding. During the morphotype transformation, the amount and composition of lipid droplets changed greatly, suggesting their important roles in these events. Overall, we provide a morphological and ultrastructural characterization of O. sinensis vegetative development within the hemocoel of T. xiaojinensis, identify and name the prehypha fungal cell type in entomopathogenic fungi for the first time, and conclude that O. sinensis infection causes sustained immunosuppression in T. xiaojinensis.}, keywords = {ultrastructure; encapsulation; Entomopathogenic fungus; Ophiocordyceps sinensis; phenoloxidase; Morphotype transformation}, year = {2020}, eissn = {1096-0805} } @article{MTMT:31868549, title = {Metabolic control of cellular immune-competency by odors in drosophila}, url = {https://m2.mtmt.hu/api/publication/31868549}, author = {Madhwal, S. and Shin, M. and Kapoor, A. and Goyal, M. and Joshi, M.K. and Rehman, P.M.U. and Gor, K. and Shim, J. and Mukherjee, T.}, doi = {10.7554/ELIFE.60376}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {9}, unique-id = {31868549}, issn = {2050-084X}, keywords = {metabolism; INFECTION; Immunity; olfaction; Hematopoiesis; SUCCINATE; GABA-shunt; HIFα}, year = {2020}, eissn = {2050-084X}, pages = {1-93} } @article{MTMT:31470095, title = {Adult Mosquitoes Infected with Bacteria Early in Life Have Stronger Antimicrobial Responses and More Hemocytes after Reinfection Later in Life}, url = {https://m2.mtmt.hu/api/publication/31470095}, author = {Powers, Joseph C. and Turangan, Raymar and Joosse, Bryan A. and Hillyer, Julian F.}, doi = {10.3390/insects11060331}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {11}, unique-id = {31470095}, abstract = {The immunological strategies employed by insects to overcome infection vary with the type of infection and may change with experience. We investigated how a bacterial infection in the hemocoel of the African malaria mosquito,Anopheles gambiae, prepares the immune system to face a subsequent bacterial infection. For this, adult female mosquitoes were separated into three groups-unmanipulated, injured, or infected withEscherichia coli-and five days later all the mosquitoes were infected with a different strain ofE. coli. We found that an injury or a bacterial infection early in life enhances the ability of mosquitoes to kill bacteria later in life. This protection results in higher mosquito survival and is associated with an increased hemocyte density, altered phagocytic activity by individual hemocytes, and the increased expression of nitric oxide synthase and perhaps prophenoloxidase 6. Protection from a second infection likely occurs because of heightened immune awareness due to an already existing infection instead of memory arising from an earlier, cured infection. This study highlights the dynamic nature of the mosquito immune response and how one infection prepares mosquitoes to survive a subsequent infection.}, keywords = {PHAGOCYTOSIS; SURVIVAL; INSECT; Immunity; nitric oxide synthase; Anopheles gambiae; prophenoloxidase}, year = {2020}, eissn = {2075-4450} } @article{MTMT:31468577, title = {Comparative RNA-Seq analyses ofDrosophilaplasmatocytes reveal gene specific signatures in response to clean injury and septic injury}, url = {https://m2.mtmt.hu/api/publication/31468577}, author = {Ramond, Elodie and Dudzic, Jan Paul and Lemaitre, Bruno}, doi = {10.1371/journal.pone.0235294}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {15}, unique-id = {31468577}, issn = {1932-6203}, abstract = {Drosophila melanogaster's blood cells (hemocytes) play essential roles in wound healing and are involved in clearing microbial infections. Here, we report the transcriptional changes of larval plasmatocytes after clean injury or infection with the Gram-negative bacteriumEscherichia colior the Gram-positive bacteriumStaphylococcus aureuscompared to hemocytes recovered from unchallenged larvae via RNA-Sequencing. This study reveals 676 differentially expressed genes (DEGs) in hemocytes from clean injury samples compared to unchallenged samples, and 235 and 184 DEGs inE.coliandS.aureussamples respectively compared to clean injury samples. The clean injury samples showed enriched DEGs for immunity, clotting, cytoskeleton, cell migration, hemocyte differentiation, and indicated a metabolic reprogramming to aerobic glycolysis, a well-defined metabolic adaptation observed in mammalian macrophages. Microbial infections trigger significant transcription of immune genes, with significant differences between theE.coliandS.aureussamples suggesting that hemocytes have the ability to engage various programs upon infection. Collectively, our data bring new insights onDrosophilahemocyte function and open the route to post-genomic functional analysis of the cellular immune response.}, year = {2020}, eissn = {1932-6203}, orcid-numbers = {Ramond, Elodie/0000-0003-4775-7070; Lemaitre, Bruno/0000-0001-7970-1667} } @book{MTMT:32661451, title = {The M2 Macrophage}, url = {https://m2.mtmt.hu/api/publication/32661451}, isbn = {9783030504793}, author = {Röszer, Tamás}, doi = {10.1007/978-3-030-50480-9}, publisher = {Springer Netherlands}, unique-id = {32661451}, abstract = {Macrophages are core components of the innate immune system. Once activated, they may have either pro- or anti-inflammatory effects that include pathogen killing, safe disposal of apoptotic cells or tissue renewal. The activation state of macrophages is conceptualized by the so-called M1/M2 model of polarization. M2 macrophages are not simply antagonists of M1 macrophages; rather, they represent a network of tissue resident macrophages with roles in tissue development and organ homeostasis. M2 macrophages govern functions at the interfaces of immunity, tissue development and turnover, metabolism, and endocrine signaling. Dysfunction in M2 macrophages can ruin the healthy interplay between the immune system and metabolic processes, and lead to diseases such as insulin resistance, metabolic syndrome, and type 1 and 2 diabetes mellitus. Furthermore, M2 macrophages are essential for healthy tissue development and immunological self-tolerance. Worryingly, these functions of M2 macrophages can also be disrupted, resulting in tumor growth and autoimmunity. This book comprehensively discusses the biology of M2 macrophages, summarizes the current state of knowledge, and highlights key questions that remain unanswered.}, keywords = {immunology; DISEASES; lipidology; human physiology; cell biology}, year = {2020}, pages = {400-624} } @article{MTMT:31467726, title = {Subpopulation of Macrophage-Like Plasmatocytes Attenuates Systemic Growth via JAK/STAT in the Drosophila Fat Body}, url = {https://m2.mtmt.hu/api/publication/31467726}, author = {Shin, Mingyu and Cha, Nuri and Koranteng, Ferdinand and Cho, Bumsik and Shim, Jiwon}, doi = {10.3389/fimmu.2020.00063}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {11}, unique-id = {31467726}, issn = {1664-3224}, abstract = {Drosophila hemocytes, like those of mammals, are given rise from two distinctive phases during both the embryonic and larval hematopoiesis. Embryonically derived hemocytes, mostly composed of macrophage-like plasmatocytes, are largely identified by genetic markers. However, the cellular diversity and distinct functions of possible subpopulations within plasmatocytes have not been explored in Drosophila larvae. Here, we show that larval plasmatocytes exhibit differential expressions of Hemolectin (Hml) and Peroxidasin (Pxn) during development. Moreover, removal of plasmatocytes by overexpressing pro-apoptotic genes, hid and reaper in Hml-positive plasmatocytes, feeding high sucrose diet, or wasp infestation results in increased circulating hemocytes that are Hml-negative. Interestingly these Hml-negative plasmatocytes retain Pxn expression, and animals expressing Hml-negative and Pxn-positive subtype largely attenuate growth and abrogate metabolism. Furthermore, elevated levels of a cytokine, unpaired 3, are detected when Hml-positive hemocytes are ablated, which in turn activates JAK/STAT activity in several tissues including the fat body. Finally, we observed that insulin signaling is inhibited in this background, which can be recovered by concurrent loss of upd3. Overall, this study highlights heterogeneity in Drosophila plasmatocytes and a functional plasticity of each subtype, which reaffirms extension of their role beyond immunity into metabolic regulation for cooperatively maintaining internal homeostatic balance.}, keywords = {Drosophila melanogaster; Peroxidasin; insulin signaling; plasmatocytes; JAK/STAT; upd3; Hemolectin}, year = {2020}, eissn = {1664-3224}, orcid-numbers = {Koranteng, Ferdinand/0000-0002-0545-2423; Shim, Jiwon/0000-0003-2409-1130} } @article{MTMT:31468416, title = {A single-cell survey of Drosophila blood}, url = {https://m2.mtmt.hu/api/publication/31468416}, author = {Tattikota, Sudhir Gopal and Cho, Bumsik and Liu, Yifang and Hu, Yanhui and Barrera, Victor and Steinbaugh, Michael J. and Yoon, Sang-Ho and Comjean, Aram and Li, Fangge and Dervis, Franz and Hung, Ruei-Jiun and Nam, Jin-Wu and Sui, Shannan Ho and Shim, Jiwon and Perrimon, Norbert}, doi = {10.7554/eLife.54818}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {9}, unique-id = {31468416}, issn = {2050-084X}, abstract = {Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions.}, year = {2020}, eissn = {2050-084X}, orcid-numbers = {Tattikota, Sudhir Gopal/0000-0003-0318-5533; Cho, Bumsik/0000-0003-1989-0624; Yoon, Sang-Ho/0000-0003-2611-5554} } @article{MTMT:31470097, title = {A novel site of haematopoiesis and appearance and dispersal of distinct haemocyte types in the Manduca sexta embryo (Insecta, Lepidoptera)}, url = {https://m2.mtmt.hu/api/publication/31470097}, author = {von Bredow, Yvette M. and von Bredow, Christoph-Ruediger and Trenczek, Tina E.}, doi = {10.1016/j.dci.2020.103722}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {111}, unique-id = {31470097}, issn = {0145-305X}, abstract = {With a set of haemocyte specific markers novel findings on haematopoiesis in the Manduca sexta embryo are presented. We identify a hitherto unknown paired haematopoietic cluster, the abdominal haemocyte cluster in abdominal segment 7 (A7-HCC). These clusters are localised at distinct positions and are established at around katatrepsis. Later in embryogenesis, the A7-HCCs disintegrate, thereby releasing numerous embryonic plasmatocytes which disperse both anteriorly and posteriorly. These cells follow stereotypic migration routes projecting anteriorly. The thoracic larval haematopoietic organs are established at around midembryogenesis. We identify embryonic oenocytoids in the M. sexta embryo for the first time. They appear in the head region roughly at the same time as the A7-HCCs occur and successively disperse in the body cavity during development. Localisation of the prophenoloxidase (proPO) mRNA and of the proPO protein are identical. Morphological, cytometric and antigenic traits show three independently generated haemocyte types during embryogenesis.}, keywords = {Embryonic insect haematopoiesis; Transient haematopoietic sites; Tobacco hornworm (Manduca sexta) embryonic hemocyte types; (Pro-)phenol oxidase containing embryonic oenocytoids; Embryonic plasmatocytes; Abdominal hemocyte cluster}, year = {2020}, eissn = {1879-0089} } @article{MTMT:30901165, title = {Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity}, url = {https://m2.mtmt.hu/api/publication/30901165}, author = {Bailetti, Alessandro A. and Negron-Pineiro, Lenny J. and Dhruva, Vishal and Harsh, Sneh and Lu, Sean and Bosula, Aisha and Bach, Erika A.}, doi = {10.1242/dmm.038679}, journal-iso = {DIS MODEL MECH}, journal = {DISEASE MODELS & MECHANISMS}, volume = {12}, unique-id = {30901165}, issn = {1754-8403}, abstract = {Myeloproliferative neoplasms (MPNs) are clonal hematopoietic disorders that cause excessive production of myeloid cells. Most MPN patients have a point mutation in JAK2 (JAK2(V617F)), which encodes a dominant-active kinase that constitutively triggers JAK/STAT signaling. In Drosophila, this pathway is simplified, with a singleJAK, Hopscotch (Hop), and a single STAT transcription factor, Stat92E. The hop(Tumorous-lethal) [hop(Tum)] allele encodes a dominant-active kinase that induces sustained Stat92E activation. Like MPN patients, hop(Tum) mutants have significantly more myeloid cells, which form invasive tumors. Through an unbiased genetic screen, we found that heterozygosity for Enhancer of Polycomb [E(Pc)], a component of the Tip60 lysine acetyltransferase complex (also known as KAT5 in humans), significantly increased tumor burden in hopTum animals. Hematopoietic depletion of E(Pc) or other Tip60 components in an otherwise wild-type background also induced blood cell tumors. The E(Pc) tumor phenotype was dependent on JAK/STAT activity, as concomitant depletion of hop or Stat92E inhibited tumor formation. Stat92E target genes were significantly upregulated in E(Pc)-mutant myeloid cells, indicating that loss of E(Pc) activates JAK STAT signaling. Neither the hop nor Stat92E gene was upregulated upon hematopoietic E(Pc) depletion, suggesting that the regulation of the JAK STAT pathway by E(Pc) is dependent on substrates other than histones. Indeed, E(Pc) depletion significantly increased expression of Hop protein in myeloid cells. This study indicates that E(Pc) works as a tumor suppressor by attenuating Hop protein expression and ultimately JAK STAT signaling. Since loss-of-function mutations in the human homologs of E(Pc) and Tip60 are frequently observed in cancer, our work could lead to new treatments for MPN patients.This article has an associated First Person interview with the first author of the paper.}, keywords = {DROSOPHILA; Tumor suppressor; MYELOPROLIFERATIVE NEOPLASMS; TIP60; JAK/STAT; E(Pc); Melanotic tumors; Lysine acetyltransferases}, year = {2019}, eissn = {1754-8411} } @article{MTMT:30510237, title = {Drosophila as a Genetic Model for Hematopoiesis}, url = {https://m2.mtmt.hu/api/publication/30510237}, author = {Banerjee, Utpal and Girard, Juliet R. and Goins, Lauren M. and Spratford, Carrie M.}, doi = {10.1534/genetics.118.300223}, journal-iso = {GENETICS}, journal = {GENETICS}, volume = {211}, unique-id = {30510237}, issn = {0016-6731}, abstract = {In this FlyBook chapter, we present a survey of the current literature on the development of the hematopoietic system in Drosophila. The Drosophila blood system consists entirely of cells that function in innate immunity, tissue integrity, wound healing, and various forms of stress response, and are therefore functionally similar to myeloid cells in mammals. The primary cell types are specialized for phagocytic, melanization, and encapsulation functions. As in mammalian systems, multiple sites of hematopoiesis are evident in Drosophila and the mechanisms involved in this process employ many of the same molecular strategies that exemplify blood development in humans. Drosophila blood progenitors respond to internal and external stress by coopting developmental pathways that involve both local and systemic signals. An important goal of these Drosophila studies is to develop the tools and mechanisms critical to further our understanding of human hematopoiesis during homeostasis and dysfunction.}, keywords = {DROSOPHILA; innate immunity; Hematopoiesis; stress response; Lamellocyte; Hemocyte; Plasmatocyte; lymph gland; FlyBook; crystal cell}, year = {2019}, eissn = {1943-2631}, pages = {367-417} } @article{MTMT:31072775, title = {Adult Drosophila Lack Hematopoiesis but Rely on a Blood Cell Reservoir at the Respiratory Epithelia to Relay Infection Signals to Surrounding Tissues}, url = {https://m2.mtmt.hu/api/publication/31072775}, author = {Bosch, Pablo Sanchez and Makhijani, Kalpana and Herboso, Leire and Gold, Katrina S. and Baginsky, Rowan and Woodcock, Katie J. and Alexander, Brandy and Kukar, Katelyn and Corcoran, Sean and Jacobs, Thea and Ouyang, Debra and Wong, Corinna and Ramond, Elodie J. V. and Rhiner, Christa and Moreno, Eduardo and Lemaitre, Bruno and Geissmann, Frederic and Bruckner, Katja}, doi = {10.1016/j.devcel.2019.10.017}, journal-iso = {DEV CELL}, journal = {DEVELOPMENTAL CELL}, volume = {51}, unique-id = {31072775}, issn = {1534-5807}, abstract = {The use of adult Drosophila melanogaster as a model for hematopoiesis or organismal immunity has been debated. Addressing this question, we identify an extensive reservoir of blood cells (hemocytes) at the respiratory epithelia (tracheal air sacs) of the thorax and head. Lineage tracing and functional analyses demonstrate that the majority of adult hemocytes are phagocytic macrophages (plasmatocytes) from the embryonic lineage that parallels vertebrate tissue macrophages. Surprisingly, we find no sign of adult hemocyte expansion. Instead, hemocytes play a role in relaying an innate immune response to the blood cell reservoir: through Mid signaling and the Jak/Stat pathway ligand Upd3, hemocytes act as sentinels of bacterial infection, inducing expression of the antimicrobial peptide Drosocin in respiratory epithelia and colocalizing fat body domains. Drosocin expression in turn promotes animal survival after infection. Our work identifies a multisignal relay of organismal humoral immunity, establishing adult Drosophila as model for inter-organ immunity.}, year = {2019}, eissn = {1878-1551}, pages = {787-+}, orcid-numbers = {Rhiner, Christa/0000-0001-7577-8042} } @article{MTMT:30907377, title = {Pericardin, a Drosophila collagen, facilitates accumulation of hemocytes at the heart}, url = {https://m2.mtmt.hu/api/publication/30907377}, author = {Cevik, Duygu and Acker, Meryl and Michalski, Camilla and Jacobs, J. Roger}, doi = {10.1016/j.ydbio.2019.06.006}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {454}, unique-id = {30907377}, issn = {0012-1606}, abstract = {Hematopoietic cell lineages support organismal needs by responding to positional and systemic signals that balance proliferative and differentiation events. Drosophila provides an excellent genetic model to dissect these signals, where the activity of cues in the hemolymph or substrate can be traced to determination and differentiation events of well characterized hemocyte types. Plasmatocytes in third instar larvae increase in number in response to infection and in anticipation of metamorphosis. Here we characterize hemocyte clustering, proliferation and transdifferentiation on the heart or dorsal vessel. Hemocytes accumulate on the inner foldings of the heart basement membrane, where they move with heart contraction, and are in proximity to the heart ostia and pericardial nephrocytes. The numbers of hemocytes vary, but increase transiently before pupariation, and decrease by 4 h before pupa formation. During their accumulation at the heart, plasmatocytes can proliferate and can transdifferentiate into crystal cells. Serrate expressing cells as well as lamellocyte-like, Atilla expressing ensheathing cells are associated with some, but not all hemocyte clusters. Hemocyte aggregation is enhanced by the presence of a heart specific Collagen, Pericardin, but not the associated pericardial cells. The varied and transient number of hemocytes in the pericardial compartment suggests that this is not a hematopoietic hub, but a niche supporting differentiation and rapid dispersal in response to systemic signals.}, keywords = {INFECTION; niche; extracellular matrix; Hematopoiesis; Lamellocyte; Plasmatocyte; dorsal vessel; klf15; Viking. lonely heart}, year = {2019}, eissn = {1095-564X}, pages = {52-65} } @article{MTMT:30907275, title = {Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process}, url = {https://m2.mtmt.hu/api/publication/30907275}, author = {Kim-Jo, Chami and Gatti, Jean-Luc and Poirie, Marylene}, doi = {10.3389/fphys.2019.00603}, journal-iso = {FRONT PHYSIOL}, journal = {FRONTIERS IN PHYSIOLOGY}, volume = {10}, unique-id = {30907275}, abstract = {Host-parasitoid interactions are among the most studied interactions between invertebrates because of their fundamental interest - the evolution of original traits in parasitoids - and applied, parasitoids being widely used in biological control. Immunity, and in particular cellular immunity, is central in these interactions, the host encapsulation response being specific for large foreign bodies such as parasitoid eggs. Although already well studied in this species, recent data on Drosophila melanogaster have unquestionably improved knowledge of invertebrate cellular immunity. At the same time, the venomics of parasitoids has expanded, notably those of Drosophila. Here, we summarize and discuss these advances, with a focus on an emerging "time-dependent" view of interactions outcome at the intra- and interspecific level. We also present issues still in debate and prospects for study. Data on the Drosophila-parasitoid model paves the way to new concepts in insect immunity as well as parasitoid wasp strategies to overcome it.}, keywords = {VENOM; DROSOPHILA; Immunity; encapsulation; Hematopoiesis; Parasitoid wasp; Leptopilina}, year = {2019}, eissn = {1664-042X} } @article{MTMT:30912668, title = {Directed differentiation of granular cells from crayfish hematopoietic tissue cells}, url = {https://m2.mtmt.hu/api/publication/30912668}, author = {Li, Fang and Xu, Limei and Hui, Xuan and Huang, Wanzhen and Yang, Feng}, doi = {10.1016/j.fsi.2019.02.054}, journal-iso = {FISH SHELLFISH IMMUN}, journal = {FISH AND SHELLFISH IMMUNOLOGY}, volume = {88}, unique-id = {30912668}, issn = {1050-4648}, abstract = {Hemocytes are the major immune cells of crustaceans. New hemocyte production is required throughout the life cycle of these animals to maintain a functional immune system. The mechanism of crustacean hematopoiesis has just begun to be understood and new methods are needed for the investigation of this process. Here we report the directed differentiation of granular cells (GCs) from the hematopoietic tissue (HPT) cells of Cherax quadricarinatus in vitro. We started by providing the cultured HPT cells with different additives to induce possible differentiation. We found that crayfish muscle extract greatly promoted the physical status of the cells and induced the formation of refractile cytoplasmic granules. The transcription of marker genes and the production of functional prophenoloxidase further confirmed the formation of mature GCs. In our experiments, young GCs usually started to develop in 2 weeks post induction and over 60% of the cells became mature within 3-4 weeks. This is the first time that the fully differentiation of crustacean hemocytes is accomplished in vitro. It provides a powerful tool for in-depth study of crustacean hematopoiesis.}, keywords = {DIFFERENTIATION; CRAYFISH; Hematopoiesis; granular cell; Hematopoietic tissue cell}, year = {2019}, eissn = {1095-9947}, pages = {28-35} } @article{MTMT:30641961, title = {Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/30641961}, author = {Melcarne, Claudia and Ramond, Elodie and Dudzic, Jan and Bretscher, Andrew and Kurucz, Judit Éva and Andó, István and Lemaitre, Bruno}, doi = {10.1111/febs.14857}, journal-iso = {FEBS J}, journal = {FEBS JOURNAL}, volume = {286}, unique-id = {30641961}, issn = {1742-464X}, year = {2019}, eissn = {1742-4658}, pages = {2670-2691}, orcid-numbers = {Andó, István/0000-0002-4648-9396} } @article{MTMT:31149702, title = {SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila}, url = {https://m2.mtmt.hu/api/publication/31149702}, author = {Paddibhatla, I. and Gautam, D.K. and Mishra, R.K.}, doi = {10.1016/j.ydbio.2019.08.008}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {456}, unique-id = {31149702}, issn = {0012-1606}, abstract = {Proper genetic and epigenetic regulation is necessary to maintain the identity and integrity of cells. Enzymes involved in post-transcriptional modifications of histones are key factors in epigenetic mechanisms. Such modifications are also gaining importance for their role in growth and development of cancer. SETDB1 catalyzes the epigenetic mark of lysine-9 methylation of histone-3. In this study, we explored the role of SETDB1 in Drosophila hematopoiesis. We show that SETDB1 controls the differentiation of matured blood cells in wandering third instar larvae. There are three matured blood cells in wild type Drosophila melanogaster: plasmatocytes, crystal cells and lamellocytes. We found that loss-of-function mutants of SETDB1 show hematopoietic defects; increased blood cell proliferation, decreased number of crystal cells, greater differentiation of blood cells into lamellocytes, dysplasia of the anterior lobes of lymph gland and presence of hematopoietic tumors. Cell type specific knockdown of SETDB1 provided similar phenotype i.e., decreased number of crystal cells and an increase in lamellocyte differentiation. In animals with loss of function of SETDB1, Notch pathway was downregulated. Further, over-expression of SETDB1 in blood cells resulted in an increase in the number of crystal cells. This increase is accompanied with an increase in the number of NotchICD expressing cells. We therefore performed genetic rescue using UAS-GAL4 system to rescue loss of function SETDB1 mutants. Our data show that the rescued larvae carrying a wild type copy of SETDB1 in mutant background are devoid of blood tumors. We have identified a novel dual function of SETDB1 methylatransferase as a critical regulator of two of the matured hemocytes, crystal cells and lamellocytes. We propose a novel role of SETDB1 in modulating the differentiation of crystal cells and lamellocytes from a common progenitor and underscore the importance of SETDB1 in Drosophila blood tumor suppression. © 2019 The Authors}, keywords = {ARTICLE; Cell Differentiation; priority journal; controlled study; nonhuman; cell proliferation; Drosophila melanogaster; unclassified drug; Blood cells; blood cell; plasma cell; hematologic malignancy; loss of function mutation; dysplasia; Hematopoiesis; Hematopoiesis; Notch signaling; Lamellocyte; histone methyltransferase; SETDB1; crystal cells; lamellocytes; lymph glands; crystal cell; Microtumors; SETDB1 protein}, year = {2019}, eissn = {1095-564X}, pages = {74-85} } @article{MTMT:30509856, title = {Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/30509856}, author = {Valanne, Susanna and Salminen, Tiina S. and Jarvela-Stolting, Mirva and Vesala, Laura and Ramet, Mika}, doi = {10.1371/journal.ppat.1007504}, journal-iso = {PLOS PATHOG}, journal = {PLOS PATHOGENS}, volume = {15}, unique-id = {30509856}, issn = {1553-7366}, abstract = {Non-coding RNAs have important roles in regulating physiology, including immunity. Here, we performed transcriptome profiling of immune-responsive genes in Drosophila melanogaster during a Gram-positive bacterial infection, concentrating on long non-coding RNA (lncRNA) genes. The gene most highly induced by a Micrococcus luteus infection was CR44404, named Induced by Infection (lincRNA-IBIN). lincRNA-IBIN is induced by both Gram-positive and Gram-negative bacteria in Drosophila adults and parasitoid wasp Leptopilina boulardi in Drosophila larvae, as well as by the activation of the Toll or the Imd pathway in unchallenged flies. We show that upon infection, lincRNA-IBIN is expressed in the fat body, in hemocytes and in the gut, and its expression is regulated by NF-B signaling and the chromatin modeling brahma complex. In the fat body, overexpression of lincRNA-IBIN affected the expression of Toll pathway -mediated genes. Notably, overexpression of lincRNA-IBIN in unchallenged flies elevated sugar levels in the hemolymph by enhancing the expression of genes important for glucose retrieval. These data show that lncRNA genes play a role in Drosophila immunity and indicate that lincRNA-IBIN acts as a link between innate immune responses and metabolism.}, year = {2019}, eissn = {1553-7374} } @article{MTMT:30585796, title = {Headcase is a Repressor of Lamellocyte Fate in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/30585796}, author = {Varga, Gergely István and Csordás, Gábor and Cinege, Gyöngyi Ilona and Jankovics, Ferenc and Sinka, Rita and Kurucz, Judit Éva and Andó, István and Honti, Viktor}, doi = {10.3390/genes10030173}, journal-iso = {GENES-BASEL}, journal = {GENES}, volume = {10}, unique-id = {30585796}, issn = {2073-4425}, abstract = {Due to the evolutionary conservation of the regulation of hematopoiesis, Drosophila provides an excellent model organism to study blood cell differentiation and hematopoietic stem cell (HSC) maintenance. The larvae of Drosophila melanogaster respond to immune induction with the production of special effector blood cells, the lamellocytes, which encapsulate and subsequently kill the invader. Lamellocytes differentiate as a result of a concerted action of all three hematopoietic compartments of the larva: the lymph gland, the circulating hemocytes, and the sessile tissue. Within the lymph gland, the communication of the functional zones, the maintenance of HSC fate, and the differentiation of effector blood cells are regulated by a complex network of signaling pathways. Applying gene conversion, mutational analysis, and a candidate based genetic interaction screen, we investigated the role of Headcase (Hdc), the homolog of the tumor suppressor HECA in the hematopoiesis of Drosophila. We found that naive loss-of-function hdc mutant larvae produce lamellocytes, showing that Hdc has a repressive role in effector blood cell differentiation. We demonstrate that hdc genetically interacts with the Hedgehog and the Decapentaplegic pathways in the hematopoietic niche of the lymph gland. By adding further details to the model of blood cell fate regulation in the lymph gland of the larva, our findings contribute to the better understanding of HSC maintenance.}, keywords = {DIFFERENTIATION; DROSOPHILA; innate immunity; blood cell; niche; Hematopoiesis; Hemocyte}, year = {2019}, eissn = {2073-4425}, orcid-numbers = {Varga, Gergely István/0000-0001-9073-5788; Csordás, Gábor/0000-0001-6871-6839; Sinka, Rita/0000-0003-4040-4184; Andó, István/0000-0002-4648-9396} } @article{MTMT:30907274, title = {Venom Atypical Extracellular Vesicles as Interspecies Vehicles of Virulence Factors Involved in Host Specificity: The Case of a Drosophila Parasitoid Wasp}, url = {https://m2.mtmt.hu/api/publication/30907274}, author = {Wan, Bin and Goguet, Emilie and Ravallec, Marc and Pierre, Olivier and Lemauf, Severine and Volkoff, Anne-Nathalie and Gatti, Jean-Luc and Poirie, Marylone}, doi = {10.3389/fimmu.2019.01688}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {10}, unique-id = {30907274}, issn = {1664-3224}, abstract = {Endoparasitoid wasps, which lay eggs inside the bodies of other insects, use various strategies to protect their offspring from the host immune response. The hymenopteran species of the genus Leptopilina, parasites of Drosophila, rely on the injection of a venom which contains proteins and peculiar vesicles (hereafter venosomes). We show here that the injection of purified L. boulardi venosomes is sufficient to impair the function of the Drosophila melanogaster lamellocytes, a hemocyte type specialized in the defense against wasp eggs, and thus the parasitic success of the wasp. These venosomes seem to have a unique extracellular biogenesis in the wasp venom apparatus where they acquire specific secreted proteins/virulence factors and act as a transport system to deliver these compounds into host lamellocytes. The level of venosomes entry into lamellocytes of different Drosophila species was correlated with the rate of parasitism success of the wasp, suggesting that this venosome-cell interaction may represent a new evolutionary level of host-parasitoid specificity.}, keywords = {DROSOPHILA; Virulence; Immunity; Lamellocyte; Parasitoid wasp; Leptopilina; venosomes}, year = {2019}, eissn = {1664-3224} } @article{MTMT:30510582, title = {Hedgehog signaling from the Posterior Signaling Center maintains U-shaped expression and a prohemocyte population in Drosophila}, url = {https://m2.mtmt.hu/api/publication/30510582}, author = {Baldeosingh, Rajkumar and Gao, Hongjuan and Wu, Xiaorong and Fossett, Nancy}, doi = {10.1016/j.ydbio.2018.06.020}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {441}, unique-id = {30510582}, issn = {0012-1606}, abstract = {Hematopoietic progenitor choice between multipotency and differentiation is tightly regulated by intrinsic factors and extrinsic signals from the surrounding microenvironment. The Drosophila melanogaster hematopoietic lymph gland has emerged as a powerful tool to investigate mechanisms that regulate hematopoietic progenitor choice in vivo. The lymph gland contains progenitor cells, which share key characteristics with mammalian hematopoietic progenitors such as quiescence, multipotency and niche dependence. The lymph gland is zonally arranged, with progenitors located in medullary zone, differentiating cells in the cortical zone, and the stem cell niche or Posterior Signaling Center (PSC) residing at the base of the medullary zone (MZ). This arrangement facilitates investigations into how signaling from the microenvironment controls progenitor choice. The Drosophila Friend of GATA transcriptional regulator, U-shaped, is a conserved hematopoietic regulator. To identify additional novel intrinsic and extrinsic regulators that interface with U-shaped to control hematopoiesis, we conducted an in vivo screen for factors that genetically interact with u-shaped. Smoothened, a downstream effector of Hedgehog signaling, was one of the factors identified in the screen. Here we report our studies that characterized the relationship between Smoothened and U-shaped. We showed that the PSC and Hedgehog signaling are required for U-shaped expression and that U-shaped is an important intrinsic progenitor regulator. These observations identify a potential link between the progenitor regulatory machinery and extrinsic signals from the PSC. Furthermore, we showed that both Hedgehog signaling and the PSC are required to maintain a subpopulation of progenitors. This led to a delineation of PSC-dependent versus PSC-independent progenitors and provided further evidence that the MZ progenitor population is heterogeneous. Overall, we have identified a connection between a conserved hematopoietic master regulator and a putative stem cell niche, which adds to our understanding of how signals from the microenvironment regulate progenitor multipotency.}, year = {2018}, eissn = {1095-564X}, pages = {132-145} } @article{MTMT:27602855, title = {Embryonic hematopoiesis modulates the inflammatory response and larval hematopoiesis in Drosophila}, url = {https://m2.mtmt.hu/api/publication/27602855}, author = {Bazzi, Wael and Cattenoz, Pierre B and Delaporte, Claude and Dasari, Vasanthi and Sakr, Rosy and Yuasa, Yoshihiro and Giangrande, Angela}, doi = {10.7554/eLife.34890.001}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {7}, unique-id = {27602855}, issn = {2050-084X}, abstract = {Recent lineage tracing analyses have significantly improved our understanding of immune system development and highlighted the importance of the different hematopoietic waves. The current challenge is to understand whether these waves interact and whether this affects the function of the immune system. Here we report a molecular pathway regulating the immune response and involving the communication between embryonic and larval hematopoietic waves in Drosophila. Down-regulating the transcription factor Gcm specific to embryonic hematopoiesis enhances the larval phenotypes induced by over-expressing the pro-inflammatory Jak/Stat pathway or by wasp infestation. Gcm works by modulating the transduction of the Upd cytokines to the site of larval hematopoiesis and hence the response to chronic Jak/Stat overexpression and acute wasp infestation immune challenges. Thus, homeostatic interactions control the function of the immune system in physiology and pathology. Our data also indicate that a transiently expressed developmental pathway has a long-lasting effect on the immune response. © Bazzi et al.}, year = {2018}, eissn = {2050-084X} } @article{MTMT:30510499, title = {From Drosophila Blood Cells to Human Leukemia}, url = {https://m2.mtmt.hu/api/publication/30510499}, author = {Boulet, Manon and Miller, Marion and Vandel, Laurence and Waltzer, Lucas}, doi = {10.1007/978-981-13-0529-0_11}, journal-iso = {ADV EXP MED BIOL}, journal = {ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY}, volume = {1076}, unique-id = {30510499}, issn = {0065-2598}, abstract = {The hematopoietic system plays a critical role in establishing the proper response against invading pathogens or in removing cancerous cells. Furthermore, deregulations of the hematopoietic differentiation program are at the origin of numerous diseases including leukemia. Importantly, many aspects of blood cell development have been conserved from human to Drosophila. Hence, Drosophila has emerged as a potent genetic model to study blood cell development and leukemia in vivo. In this chapter, we give a brief overview of the Drosophila hematopoietic system, and we provide a protocol for the dissection and the immunostaining of the larval lymph gland, the most studied hematopoietic organ in Drosophila. We then focus on the various paradigms that have been used in fly to investigate how conserved genes implicated in leukemogenesis control blood cell development. Specific examples of Drosophila models for leukemia are presented, with particular attention to the most translational ones. Finally, we discuss some limitations and potential improvements of Drosophila models for studying blood cell cancer.}, keywords = {LEUKEMIA; DROSOPHILA; SCREEN; Hematopoiesis}, year = {2018}, eissn = {2214-8019}, pages = {195-214} } @article{MTMT:30425005, title = {Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis}, url = {https://m2.mtmt.hu/api/publication/30425005}, author = {Hiroyasu, Aoi and DeWitt, David C. and Goodman, Alan G.}, doi = {10.3791/57077}, journal-iso = {JOVE-J VIS EXP}, journal = {JOVE-JOURNAL OF VISUALIZED EXPERIMENTS}, unique-id = {30425005}, issn = {1940-087X}, abstract = {During the pathogenic infection of Drosophila melanogaster, hemocytes play an important role in the immune response throughout the infection. Thus, the goal of this protocol is to develop a method to visualize the pathogen invasion in a specific immune compartment of flies, namely hemocytes. Using the method presented here, up to 3 x 10(6) live hemocytes can be obtained from 200 Drosophila 3rd instar larvae in 30 min for ex vivo infection. Alternatively, hemocytes can be infected in vivo through injection of 3rd instar larvae followed by hemocyte extraction up to 24 h post-infection. These infected primary cells were fixed, stained, and imaged using confocal microscopy. Then, 3D representations were generated from the images to definitively show pathogen invasion. Additionally, high-quality RNA for qRT-PCR can be obtained for the detection of pathogen mRNA following infection, and sufficient protein can be extracted from these cells for Western blot analysis. Taken together, we present a method for definite reconciliation of pathogen invasion and confirmation of infection using bacterial and viral pathogen types and an efficient method for hemocyte extraction to obtain enough live hemocytes from Drosophila larvae for ex vivo and in vivo infection experiments.}, keywords = {confocal microscopy; Listeria monocytogenes; Coxiella burnetii; Issue 135; Immunology and Infection; Pathogen invasion; hemocyte extraction; Invertebrate iridescent virus-6; IIV6}, year = {2018}, eissn = {1940-087X} } @article{MTMT:31149601, title = {Detecting proliferation of adult hemocytes in Drosophila by BrdU incorporation [version 1; referees: 2 approved]}, url = {https://m2.mtmt.hu/api/publication/31149601}, author = {Mandal, L. and Ghosh, S. and Mandal, S.}, doi = {10.12688/wellcomeopenres.14560.1}, journal-iso = {WELLCOME OPEN RESEARCH}, journal = {WELLCOME OPEN RESEARCH}, volume = {3}, unique-id = {31149601}, issn = {2398-502X}, abstract = {Drosophila and mammalian hematopoiesis share several similarities that ranges from phases to the battery of transcription factors and signaling molecules that execute this process. These resounding similarities along with the rich genetic tools available in fruitfly makes it a popular invertebrate model to study blood cell development both during normal and aberrant conditions. The larval system is the most extensively studied to date. Several studies have shown that these hemocytes just like mammalian counterpart proliferate and get routinely regenerated upon infection. However, employing the same protocol it was concluded that blood cell proliferation although abundant in larval stages is absent in adult fruitfly. The current protocol describes the strategies that can be employed to document the hemocyte proliferation in adulthood. The fact that a subset of blood cells tucked away in the hematopoietic hub are not locked in senescence, rather they still harbour the proliferative capacity to tide over challenges was successfully demonstrated by this method. Although we have adopted bacterial infection as a bait to evoke this proliferative capacity of the hemocytes, we envision that it can also efficiently characterize the proliferative responses of hemocytes in tumorigenic conditions as well as scenarios of environmental and metabolic stresses during adulthood. © 2018 Ghosh S et al.}, keywords = {INFECTION; PROLIFERATION; MACROPHAGE; Hematopoiesis; Adult Drosophila}, year = {2018} } @article{MTMT:27347640, title = {Mediation of inducible nitric oxide and immune-reactive lysozymes biosynthesis by eicosanoid and biogenic amines in flesh flies}, url = {https://m2.mtmt.hu/api/publication/27347640}, author = {Mohamed, Amr A and Ali, Mona M and Dorrah, Moataza A and Bassal, Taha T M}, doi = {10.1017/S1742758417000315}, journal-iso = {INT J TROP INSECT SC}, journal = {INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE}, volume = {38}, unique-id = {27347640}, issn = {1742-7584}, year = {2018}, eissn = {1742-7592}, pages = {93-104} } @article{MTMT:27602854, title = {Mechanical stress to Drosophila larvae stimulates a cellular immune response through the JAK/STAT signaling pathway}, url = {https://m2.mtmt.hu/api/publication/27602854}, author = {Tokusumi, Yumiko and Tokusumi, Tsuyoshi and Schulz, Robert A}, doi = {10.1016/j.bbrc.2018.05.192}, journal-iso = {BIOCHEM BIOPH RES CO}, journal = {BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS}, volume = {502}, unique-id = {27602854}, issn = {0006-291X}, year = {2018}, eissn = {1090-2104}, pages = {415-421}, orcid-numbers = {Tokusumi, Tsuyoshi/0000-0001-9494-1888} } @article{MTMT:26891561, title = {A Genetic Screen Reveals an Unexpected Role for Yorkie Signaling in JAK/STAT-Dependent Hematopoietic Malignancies in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/26891561}, author = {Anderson, Abigail M and Bailetti, Alessandro A and Rodkin, Elizabeth and De, Atish and Bach, Erika A}, doi = {10.1534/g3.117.044172}, journal-iso = {G3-GENES GENOM GENET}, journal = {G3-GENES GENOMES GENETICS}, volume = {7}, unique-id = {26891561}, issn = {2160-1836}, year = {2017}, eissn = {2160-1836}, pages = {2427-2438} } @{MTMT:26274581, title = {Molecular Control of Actin Dynamics In Vivo: Insights from Drosophila}, url = {https://m2.mtmt.hu/api/publication/26274581}, author = {Brüser, Lena and Bogdan, Sven}, booktitle = {The Actin Cytoskeleton}, doi = {10.1007/164_2016_33}, publisher = {Springer Netherlands}, unique-id = {26274581}, year = {2017}, pages = {285-310} } @article{MTMT:27055182, title = {dOCRL maintains immune cell quiescence by regulating endosomal traffic}, url = {https://m2.mtmt.hu/api/publication/27055182}, author = {Del Signore, Steven J and Biber, Sarah A and Lehmann, Katherine S and Heimler, Stephanie R and Rosenfeld, Benjamin H and Eskin, Tania L and Sweeney, Sean T and Rodal, Avital A}, doi = {10.1371/journal.pgen.1007052}, journal-iso = {PLOS GENET}, journal = {PLOS GENETICS}, volume = {13}, unique-id = {27055182}, issn = {1553-7390}, year = {2017}, eissn = {1553-7404} } @article{MTMT:26891459, title = {Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps}, url = {https://m2.mtmt.hu/api/publication/26891459}, author = {Dostalova, Anna and Rommelaere, Samuel and Poidevin, Mickael and Lemaitre, Bruno}, doi = {10.1186/s12915-017-0408-0}, journal-iso = {BMC BIOL}, journal = {BMC BIOLOGY}, volume = {15}, unique-id = {26891459}, issn = {1741-7007}, year = {2017}, eissn = {1741-7007}, orcid-numbers = {Lemaitre, Bruno/0000-0001-7970-1667} } @article{MTMT:26536389, title = {Advances in Myeloid-Like Cell Origins and Functions in the Model Organism Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/26536389}, author = {El, Chamy Laure and Matt, Nicolas and Reichhart, Jean-Marc}, doi = {10.1128/microbiolspec.MCHD-0038-2016}, journal-iso = {MICROBIOL SPEC}, journal = {MICROBIOLOGY SPECTRUM}, volume = {5}, unique-id = {26536389}, issn = {2165-0497}, year = {2017}, eissn = {2165-0497} } @article{MTMT:3249751, title = {Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes}, url = {https://m2.mtmt.hu/api/publication/3249751}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordás, Gábor and Török, Tibor and Medzihradszky F., Katalin and Darula, Zsuzsanna and Andó, István and Kurucz, Judit Éva}, doi = {10.1016/j.dci.2017.07.013}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {76}, unique-id = {3249751}, issn = {0145-305X}, year = {2017}, eissn = {1879-0089}, pages = {403-411}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Török, Tibor/0000-0002-2128-1126; Andó, István/0000-0002-4648-9396} } @article{MTMT:26674050, title = {RUNX in invertebrates}, url = {https://m2.mtmt.hu/api/publication/26674050}, author = {Hughes, S and Woollard, A}, doi = {10.1007/978-981-10-3233-2_1}, journal-iso = {ADV EXP MED BIOL}, journal = {ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY}, volume = {962}, unique-id = {26674050}, issn = {0065-2598}, year = {2017}, eissn = {2214-8019}, pages = {3-18} } @article{MTMT:26891460, title = {Modulation of occluding junctions alters the hematopoietic niche to trigger immune activation}, url = {https://m2.mtmt.hu/api/publication/26891460}, author = {Khadilkar, Rohan J and Vogl, Wayne and Goodwin, Katharine and Tanentzapf, Guy}, doi = {10.7554/eLife.28081}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {6}, unique-id = {26891460}, issn = {2050-084X}, year = {2017}, eissn = {2050-084X} } @article{MTMT:27260897, title = {Reactive oxygen species-dependent Toll/NF-kappa B activation in the Drosophila hematopoietic niche confers resistance to wasp parasitism}, url = {https://m2.mtmt.hu/api/publication/27260897}, author = {Louradour, Isabelle and Sharma, Anurag and Morin-Poulard, Ismael and Letourneau, Manon and Vincent, Alain and Crozatier, Michele and Vanzo, Nathalie}, doi = {10.7554/eLife.25496}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {6}, unique-id = {27260897}, issn = {2050-084X}, year = {2017}, eissn = {2050-084X} } @article{MTMT:26674052, title = {Life-history strategy determines constraints on immune function}, url = {https://m2.mtmt.hu/api/publication/26674052}, author = {Parker, BJ and Barribeau, SM and Laughton, AM and Griffin, LH and Gerardo, NM}, doi = {10.1111/1365-2656.12657}, journal-iso = {J ANIM ECOL}, journal = {JOURNAL OF ANIMAL ECOLOGY}, volume = {86}, unique-id = {26674052}, issn = {0021-8790}, year = {2017}, eissn = {1365-2656}, pages = {473-483} } @article{MTMT:26536387, title = {Screening and Analysis of Janelia FlyLight Project Enhancer-Gal4 Strains Identifies Multiple Gene Enhancers Active During Hematopoiesis in Normal and Wasp-Challenged Drosophila Larvae}, url = {https://m2.mtmt.hu/api/publication/26536387}, author = {Tokusumi, Tsuyoshi and Tokusumi, Yumiko and Brahier, Mark S and Lam, Victoria and Stoller-Conrad, Jessica R and Kroeger, Paul T and Schulz, Robert A}, doi = {10.1534/g3.116.034439}, journal-iso = {G3-GENES GENOM GENET}, journal = {G3-GENES GENOMES GENETICS}, volume = {7}, unique-id = {26536387}, issn = {2160-1836}, year = {2017}, eissn = {2160-1836}, pages = {437-448} } @article{MTMT:26714786, title = {C-type lectin interacting with beta-integrin enhances hemocytic encapsulation in the cotton bollworm, Helicoverpa armigera}, url = {https://m2.mtmt.hu/api/publication/26714786}, author = {Wang, Pan and Zhuo, Xiao-Rong and Tang, Lin and Liu, Xu-Sheng and Wang, Yu-Feng and Wang, Guo-Xiu and Yu, Xiao-Qiang and Wang, Jia-Lin}, doi = {10.1016/j.ibmb.2017.05.005}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {86}, unique-id = {26714786}, issn = {0965-1748}, year = {2017}, eissn = {1879-0240}, pages = {29-40} } @article{MTMT:26377205, title = {The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas}, url = {https://m2.mtmt.hu/api/publication/26377205}, author = {Wang, Weilin and Li, Meijia and Wang, Lingling and Chen, Hao and Liu, Zhaoqun and Jia, Zhihao and Qiu, Limei and Song, Linsheng}, doi = {10.1016/j.dci.2016.09.017}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {67}, unique-id = {26377205}, issn = {0145-305X}, year = {2017}, eissn = {1879-0089}, pages = {221-228} } @article{MTMT:26698809, title = {Macrophage Functions in Tissue Patterning and Disease: New Insights from the Fly}, url = {https://m2.mtmt.hu/api/publication/26698809}, author = {Wood, Will and Martin, Paul}, doi = {10.1016/j.devcel.2017.01.001}, journal-iso = {DEV CELL}, journal = {DEVELOPMENTAL CELL}, volume = {40}, unique-id = {26698809}, issn = {1534-5807}, year = {2017}, eissn = {1878-1551}, pages = {221-233}, orcid-numbers = {Martin, Paul/0000-0002-2665-5086} } @article{MTMT:26878576, title = {The matrix protein Tiggrin regulates plasmatocyte maturation in Drosophila larva}, url = {https://m2.mtmt.hu/api/publication/26878576}, author = {Zhang, Chen U and Cadigan, Ken M}, doi = {10.1242/dev.149641}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {144}, unique-id = {26878576}, issn = {0950-1991}, year = {2017}, eissn = {1477-9129}, pages = {2415-2427} } @article{MTMT:3096913, title = {Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection.}, url = {https://m2.mtmt.hu/api/publication/3096913}, author = {Anderl, I and Vesala, L and Ihalainen, TO and Vanha-Aho, LM and Andó, István and Ramet, M and Hultmark, D}, doi = {10.1371/journal.ppat.1005746}, journal-iso = {PLOS PATHOG}, journal = {PLOS PATHOGENS}, volume = {12}, unique-id = {3096913}, issn = {1553-7366}, abstract = {Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail.}, year = {2016}, eissn = {1553-7374}, pages = {e1005746}, orcid-numbers = {Andó, István/0000-0002-4648-9396} } @article{MTMT:26203670, title = {Revisiting the role of the Gcm transcription factor, from master regulator to Swiss army knife}, url = {https://m2.mtmt.hu/api/publication/26203670}, author = {Cattenoz, Pierre B and Giangrande, Angela}, doi = {10.1080/19336934.2016.1212793}, journal-iso = {FLY}, journal = {FLY}, volume = {10}, unique-id = {26203670}, issn = {1933-6934}, year = {2016}, eissn = {1933-6942}, pages = {210-218} } @article{MTMT:26377269, title = {Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila}, url = {https://m2.mtmt.hu/api/publication/26377269}, author = {Dey, Nidhi Sharma and Ramesh, Parvathy and Chugh, Mayank and Mandal, Sudip and Mandal, Lolitika}, doi = {10.7554/eLife.18295}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {5}, unique-id = {26377269}, issn = {2050-084X}, year = {2016}, eissn = {2050-084X} } @article{MTMT:26016211, title = {The Friend of GATA Transcriptional Co-Regulator, U-Shaped, Is a Downstream Antagonist of Dorsal-Driven Prohemocyte Differentiation in Drosophila}, url = {https://m2.mtmt.hu/api/publication/26016211}, author = {Gao, Hongjuan and Baldeosingh, Rajkumar and Wu, Xiaorong and Fossett, Nancy}, doi = {10.1371/journal.pone.0155372}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {11}, unique-id = {26016211}, issn = {1932-6203}, year = {2016}, eissn = {1932-6203} } @article{MTMT:26203994, title = {The human Smoothened inhibitor PF-04449913 induces exit from quiescence and loss of multipotent Drosophila hematopoietic progenitor cells}, url = {https://m2.mtmt.hu/api/publication/26203994}, author = {Giordani, Giorgia and Barraco, Marilena and Giangrande, Angela and Martinelli, Giovanni and Guadagnuolo, Viviana and Simonetti, Giorgia and Perini, Giovanni and Bernardoni, Roberto}, doi = {10.18632/oncotarget.10879}, journal-iso = {ONCOTARGET}, journal = {ONCOTARGET}, volume = {7}, unique-id = {26203994}, year = {2016}, eissn = {1949-2553}, pages = {55313-55327} } @article{MTMT:25771630, title = {Insect immunology and hematopoiesis}, url = {https://m2.mtmt.hu/api/publication/25771630}, author = {Hillyer, Julian F}, doi = {10.1016/j.dci.2015.12.006}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {58}, unique-id = {25771630}, issn = {0145-305X}, year = {2016}, eissn = {1879-0089}, pages = {102-118} } @article{MTMT:3045263, title = {The raspberry Gene Is Involved in the Regulation of the Cellular Immune Response in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/3045263}, author = {Kari, Beáta and Csordás, Gábor and Honti, Viktor and Cinege, Gyöngyi Ilona and Williams, MJ and Andó, István and Kurucz, Judit Éva}, doi = {10.1371/journal.pone.0150910}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {11}, unique-id = {3045263}, issn = {1932-6203}, abstract = {Drosophila is an extremely useful model organism for understanding how innate immune mechanisms defend against microbes and parasitoids. Large foreign objects trigger a potent cellular immune response in Drosophila larva. In the case of endoparasitoid wasp eggs, this response includes hemocyte proliferation, lamellocyte differentiation and eventual encapsulation of the egg. The encapsulation reaction involves the attachment and spreading of hemocytes around the egg, which requires cytoskeletal rearrangements, changes in adhesion properties and cell shape, as well as melanization of the capsule. Guanine nucleotide metabolism has an essential role in the regulation of pathways necessary for this encapsulation response. Here, we show that the Drosophila inosine 5'-monophosphate dehydrogenase (IMPDH), encoded by raspberry (ras), is centrally important for a proper cellular immune response against eggs from the parasitoid wasp Leptopilina boulardi. Notably, hemocyte attachment to the egg and subsequent melanization of the capsule are deficient in hypomorphic ras mutant larvae, which results in a compromised cellular immune response and increased survival of the parasitoid.}, keywords = {PHAGOCYTOSIS; INHIBITORS; ACTIVATION; SCREEN; RHO; Hematopoiesis; INOSINE MONOPHOSPHATE DEHYDROGENASE; Parasitoids; SMALL GTPASES; LEPTOPILINA-BOULARDI}, year = {2016}, eissn = {1932-6203}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396} } @article{MTMT:26203993, title = {Functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts}, url = {https://m2.mtmt.hu/api/publication/26203993}, author = {League, Garrett P and Hillyer, Julian F}, doi = {10.1186/s12915-016-0305-y}, journal-iso = {BMC BIOL}, journal = {BMC BIOLOGY}, volume = {14}, unique-id = {26203993}, issn = {1741-7007}, year = {2016}, eissn = {1741-7007} } @article{MTMT:26377268, title = {Drosophila hematopoiesis under normal conditions and in response to immune stress}, url = {https://m2.mtmt.hu/api/publication/26377268}, author = {Letourneau, Manon and Lapraz, Francois and Sharma, Anurag and Vanzo, Nathalie and Waltzer, Lucas and Crozatier, Michele}, doi = {10.1002/1873-3468.12327}, journal-iso = {FEBS LETT}, journal = {FEBS LETTERS}, volume = {590}, unique-id = {26377268}, issn = {0014-5793}, year = {2016}, eissn = {1873-3468}, pages = {4034-4051} } @article{MTMT:30901168, title = {MiniCORVET is a Vps8-containing early endosomal tether in Drosophila}, url = {https://m2.mtmt.hu/api/publication/30901168}, author = {Lőrincz, Péter and Lakatos, Zsolt and Varga, Ágnes and Maruzs, Tamás and Simon-Vecsei, Zsófia Judit and Darula, Zsuzsanna and Benkő, Péter and Csordás, Gábor and Lippai, Mónika and Andó, István and Hegedűs, Krisztina and Medzihradszky F., Katalin and Takáts, Szabolcs and Juhász, Gábor}, doi = {10.7554/eLife.14226}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {5}, unique-id = {30901168}, issn = {2050-084X}, abstract = {Yeast studies identified two heterohexameric tethering complexes, which consist of 4 shared (Vps11, Vps16, Vps18 and Vps33) and 2 specific subunits: Vps3 and Vps8 (CORVET) versus Vps39 and Vps41 (HOPS). CORVET is an early and HOPS is a late endosomal tether. The function of HOPS is well known in animal cells, while CORVET is poorly characterized. Here we show that Drosophila Vps8 is highly expressed in hemocytes and nephrocytes, and localizes to early endosomes despite the lack of a clear Vps3 homolog. We find that Vps8 forms a complex and acts together with Vps16A, Dor/Vps18 and Car/Vps33A, and loss of any of these proteins leads to fragmentation of endosomes. Surprisingly, Vps11 deletion causes enlargement of endosomes, similar to loss of the HOPS-specific subunits Vps39 and Lt/Nps41. We thus identify a 4 subunit-containing miniCORVET complex as an unconventional early endosomal tether in Drosophila.}, year = {2016}, eissn = {2050-084X}, orcid-numbers = {Lőrincz, Péter/0000-0001-7374-667X; Lakatos, Zsolt/0000-0003-1900-3167; Maruzs, Tamás/0000-0001-8142-3221; Simon-Vecsei, Zsófia Judit/0000-0001-7909-4895; Benkő, Péter/0000-0002-2050-7509; Csordás, Gábor/0000-0001-6871-6839; Lippai, Mónika/0000-0002-7307-4233; Andó, István/0000-0002-4648-9396; Takáts, Szabolcs/0000-0003-2139-7740; Juhász, Gábor/0000-0001-8548-8874} } @article{MTMT:26203667, title = {The Role of Lipid Competition for Endosymbiont-Mediated Protection against Parasitoid Wasps in Drosophila}, url = {https://m2.mtmt.hu/api/publication/26203667}, author = {Paredes, Juan C and Herren, Jeremy K and Schupfer, Fanny and Lemaitre, Bruno}, doi = {10.1128/mBio.01006-16}, journal-iso = {MBIO}, journal = {MBIO}, volume = {7}, unique-id = {26203667}, issn = {2161-2129}, year = {2016}, eissn = {2150-7511} } @article{MTMT:25771631, title = {Cellular immune defenses of Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/25771631}, author = {Parsons, Brendon and Foley, Edan}, doi = {10.1016/j.dci.2015.12.019}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {58}, unique-id = {25771631}, issn = {0145-305X}, year = {2016}, eissn = {1879-0089}, pages = {95-101} } @article{MTMT:26674053, title = {Methods to examine the lymph gland and hemocytes in Drosophila larvae}, url = {https://m2.mtmt.hu/api/publication/26674053}, author = {Reimels, TA and Pfleger, CM}, doi = {10.3791/54544}, journal-iso = {JOVE-J VIS EXP}, journal = {JOVE-JOURNAL OF VISUALIZED EXPERIMENTS}, volume = {2016}, unique-id = {26674053}, issn = {1940-087X}, year = {2016}, eissn = {1940-087X} } @article{MTMT:26237558, title = {Genetic Screen in Drosophila Larvae Links ird1 Function to Toll Signaling in the Fat Body and Hemocyte Motility}, url = {https://m2.mtmt.hu/api/publication/26237558}, author = {Schmid, Martin R and Anderl, Ines and Vo, Hoa T M and Valanne, Susanna and Yang, Hairu and Kronhamn, Jesper and Ramet, Mika and Rusten, Tor Erik and Hultmark, Dan}, doi = {10.1371/journal.pone.0159473}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {11}, unique-id = {26237558}, issn = {1932-6203}, year = {2016}, eissn = {1932-6203}, orcid-numbers = {Yang, Hairu/0000-0002-9420-878X} } @article{MTMT:25573127, title = {Mosquito hemocytes preferentially aggregate and phagocytose pathogens in the periostial regions of the heart that experience the most hemolymph flow}, url = {https://m2.mtmt.hu/api/publication/25573127}, author = {Sigle, LT and Hillyer, JF}, doi = {10.1016/j.dci.2015.10.018}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {55}, unique-id = {25573127}, issn = {0145-305X}, year = {2016}, eissn = {1879-0089}, pages = {90-101} } @article{MTMT:25771633, title = {Cytokines in Drosophila immunity}, url = {https://m2.mtmt.hu/api/publication/25771633}, author = {Vanha-aho, Leena-Maija and Valanne, Susanna and Ramet, Mika}, doi = {10.1016/j.imlet.2015.12.005}, journal-iso = {IMMUNOL LETT}, journal = {IMMUNOLOGY LETTERS}, volume = {170}, unique-id = {25771633}, issn = {0165-2478}, year = {2016}, eissn = {1879-0542}, pages = {42-51} } @article{MTMT:25573126, title = {The relative abundance of hemocyte types in a polyphagous moth larva depends on diet}, url = {https://m2.mtmt.hu/api/publication/25573126}, author = {Vogelweith, F and Moret, Y and Monceau, K and Thiéry, D and Moreau, J}, doi = {10.1016/j.jinsphys.2016.02.010}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {88}, unique-id = {25573126}, issn = {0022-1910}, year = {2016}, eissn = {1879-1611}, pages = {33-39} } @article{MTMT:24796091, title = {New ways to make a blood cell}, url = {https://m2.mtmt.hu/api/publication/24796091}, author = {Anderl, Ines and Hultmark, Dan}, doi = {10.7554/eLife.06877}, journal-iso = {ELIFE}, journal = {ELIFE}, volume = {4}, unique-id = {24796091}, issn = {2050-084X}, year = {2015}, eissn = {2050-084X} }