TY - JOUR AU - Elhoseny, Manar M. M. AU - El-Didamony, Samia E. AU - Atwa, Wedad A. A. AU - Althoqapy, Azza A. AU - Gouda, Hend I. A. TI - New insights into changing honey bee (Apis mellifera) immunity molecules pattern and fatty acid esters, in responses to Ascosphaera apis infection JF - JOURNAL OF INVERTEBRATE PATHOLOGY J2 - J INVERTEBR PATHOL VL - 202 PY - 2024 PG - 8 SN - 0022-2011 DO - 10.1016/j.jip.2023.108028 UR - https://m2.mtmt.hu/api/publication/34597451 ID - 34597451 AB - Monitoring of metabolite changes could provide valuable insights into disturbances caused by an infection and furthermore, could be used to define the status of an organism as healthy or diseased and define what could be defensive elements against the infection. The present investigation conducted a gas chromatography-mass spectrometry (GC/MS) for haemolymph of larval honey bees (Apis mellifera L.) infected with the fungal pathogen Ascosphaera apis in comparison with control haemolymph non-infected insects. Results revealed that the path-ogen caused a general disturbance of metabolites detected in the haemolymph of the honey bee. The majority of metabolites identified before and after infection were fatty acid esters. The disease caused an elevation in levels of methyl oleate, methyl palmitate, and methyl stearate, respectively. Further, the disease drove to the disap-pearance of methyl palmitoleate, and methyl laurate. Conversely, methyl linolelaidate, and ethyl oleate were identified only in infected larvae. A high reduction in diisooctyl phthalate was recorded after the infection. Interestingly, antimicrobial activities were confirmed for haemolymph of infected honey bee larvae. In spite of the presence of some previously known bioactive compounds in healthy larvae there were no antimicrobial activities. LA - English DB - MTMT ER - TY - JOUR AU - Ewunkem, Akamu J. AU - Beard, A'lyiha F. AU - Justice, Brittany L. AU - Peoples, Sabrina L. AU - Meixner, Jeffery A. AU - Kemper, Watson AU - Iloghalu, Uchenna B. TI - Honeybee wings hold antibiofouling and antimicrobial clues for improved applications in health care and industries JF - AIMS MICROBIOLOGY J2 - AIMS MICROBIOLOGY VL - 9 PY - 2023 IS - 2 SP - 332 EP - 345 PG - 14 SN - 2471-1888 DO - 10.3934/microbiol.2023018 UR - https://m2.mtmt.hu/api/publication/33873118 ID - 33873118 AB - Natural surfaces with remarkable properties and functionality have become the focus of intense research. Heretofore, the natural antimicrobial properties of insect wings have inspired research into their applications. The wings of cicadas, butterflies, dragonflies, and damselflies have evolved phenomenal anti-biofouling and antimicrobial properties. These wings are covered by periodic topography ranging from highly ordered hexagonal arrays of nanopillars to intricate "Christmas-tree" like structures with the ability to kill microbes by physically rupturing the cell membrane. In contrast, the topography of honeybee wings has received less attention. The role topography plays in antibiofouling, and antimicrobial activity of honeybee wings has never been investigated. Here, through antimicrobial and electron microscopy studies, we showed that pristine honeybee wings displayed no microbes on the wing surface. Also, the wings displayed antimicrobial properties that disrupt microbial cells and inhibit their growth. The antimicrobial activities of the wings were extremely effective at inhibiting the growth of Gram-negative bacterial cells when compared to Gram-positive bacterial cells. The fore wing was effective at inhibiting the growth of Gram-negative bacteria compared to Gram-positive samples. Electron microscopy revealed that the wings were studded with an array of rough, sharp, and pointed pillars that were distributed on both the dorsal and ventral sides, which enhanced anti-biofouling and antimicrobial effects. Our findings demonstrate the potential benefits of incorporating honeybee wings nanopatterns into the design of antibacterial nanomaterials which can be translated into countless applications in healthcare and industry. LA - English DB - MTMT ER - TY - JOUR AU - Girgis, Michael M. AU - Christodoulides, Myron TI - Vertebrate and Invertebrate Animal and New In Vitro Models for Studying Neisseria Biology JF - PATHOGENS J2 - PATHOGENS VL - 12 PY - 2023 IS - 6 PG - 36 SN - 2076-0817 DO - 10.3390/pathogens12060782 UR - https://m2.mtmt.hu/api/publication/34261033 ID - 34261033 AB - The history of Neisseria research has involved the use of a wide variety of vertebrate and invertebrate animal models, from insects to humans. In this review, we itemise these models and describe how they have made significant contributions to understanding the pathophysiology of Neisseria infections and to the development and testing of vaccines and antimicrobials. We also look ahead, briefly, to their potential replacement by complex in vitro cellular models. LA - English DB - MTMT ER - TY - JOUR AU - Kaya, Serhat TI - Immunosuppressive effect of Plantago major on the innate immunity of Galleria mellonella JF - PEERJ J2 - PEERJ VL - 11 PY - 2023 PG - 23 SN - 2167-8359 DO - 10.7717/peerj.15982 UR - https://m2.mtmt.hu/api/publication/34261031 ID - 34261031 AB - Greater plantain (Plantago major), a medicinal plant species, is used in folk medicine for the treatment of various diseases in many countries of the world. Different studies have shown that the bioactive components contained in the plant have a dual effect. It was also reported that in vivo and in vitro studies showed different results. The aim of the study was to determine the effects of P. major extract on the hemocyte-mediated and humoral immune responses of the invertebrate model organism Galleria mellonella, which is widely used in immune studies. In the evaluation of these effects, total hemocyte count, encapsulation, melanization, phenoloxidase, superoxide dismutase, catalase, malondialdehyde and total protein parameters were evaluated. The results of the study showed that the total hemocyte count did not change, that the encapsulation responses decreased, that the melanization responses and phenoloxidase activity increased and that the superoxide dismutase activity decreased. As a result, it was determined that high doses of P. major had negative effects on cell-mediated immunity and antioxidant defence and positive effects on melanization. High doses and continuous use of P. major may have negative effects on living things. LA - English DB - MTMT ER - TY - JOUR AU - Li, Miaomiao AU - Zhang, Jihong AU - Qin, Qilian AU - Zhang, Huan AU - Li, Xuan AU - Wang, Hongtuo AU - Meng, Qian TI - Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection JF - MICROORGANISMS J2 - MICROORGANISMS VL - 11 PY - 2023 IS - 9 PG - 17 SN - 2076-2607 DO - 10.3390/microorganisms11092361 UR - https://m2.mtmt.hu/api/publication/34261032 ID - 34261032 AB - Ophiocordyceps sinensis exhibits more than 5 months of vegetative growth in Thitarodes xiaojinensis hemocoel. The peculiar development process of O. sinensis has been elucidated through morphological observation and omics technology; however, little information has been reported regarding the changes that occur in the host T. xiaojinensis. The RNA sequencing data showed that when O. sinensis blastospores were in the proliferative stage, the greatest change in the infected larval fat body was the selectively upregulated immune recognition and antimicrobial peptide genes. When O. sinensis blastospores were in the stationary stage, the immune pathways of T. xiaojinensis reverted to normal levels, which coincides with the successful settlement of O. sinensis. Pathway enrichment analysis showed a higher expression of genes involved in energy metabolism pathway in this stage. Metabolomic analyses revealed a reduction of amino acids and lipids in hemolymph, but an upregulation of lipids in the fat body of the host larvae after O. sinensis infection. We present the first transcriptome integrated with the metabolome study of T. xiaojinensis infected by O. sinensis. It will improve our understanding of the interaction mechanisms between the host and entomopathogenic fungi, and facilitate future functional studies of genes and pathways involved in these interactions. LA - English DB - MTMT ER - TY - JOUR AU - Alejandro, Alvarado-Delgado AU - Lilia, Juarez-Palma AU - Jesus, Maritinez-Bartneche AU - Mario Henry, Rodriguez TI - The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs JF - PARASITES AND VECTORS J2 - PARASITE VECT VL - 15 PY - 2022 IS - 1 PG - 13 SN - 1756-3305 DO - 10.1186/s13071-022-05363-y UR - https://m2.mtmt.hu/api/publication/33181293 ID - 33181293 AB - Background: Antimicrobial peptides (AMPs) participate in the humoral immune response of insects eliminating invasive microorganisms. The immune deficiency pathway (IMD) and Toll are the main pathways by which the synthesis of these molecules is regulated in response to Gram-negative (IMD pathway) or Gram-positive (Toll pathway) bacteria. Various pattern-recognition receptors (PRRs) participate in the recognition of microorganisms, such as pgrp-Ic and toll, which trigger signaling cascades and activate NF-kappa B family transcription factors, such as relish, that translocate to the cell nucleus, mainly in the fat body, inducing AMP gene transcription.Methods: T. pallidipennis inhibited in Tppgrp-lc,Tptoll, and Tprelish were challenged with E. coli and M. luteus to analyze the expression of AMPs transcripts in the fat body and to execute survival assays.Results: In this work we investigated the participation of the pgrp-lc and toll receptor genes and the relish transcription factor (designated as Tppgrp-lc, Tptoll, and Tprelish), in the transcriptional regulation of defensin B, prolixicin, and lysozyme B in Triatoma pallidipennis, one of the main vectors of Chagas disease. AMP transcript abundance was higher in the fat body of blood-fed than non-fed bugs. Challenge with Escherichia coli or Micrococcus luteus induced differential increases in AMP transcripts. Additionally, silencing of Tppgrp-lc, Tptoll, and Tprelish resulted in reduced AMP transcription and survival of bugs after a bacterial challenge.Conclusions: Our findings demonstrated that the IMD and Toll pathways in T. pallidipennis preferentially respond to Gram-negative and Gram-positive bacteria, respectively, by increasing the expression of AMP transcripts, but crossinduction also occurs. LA - English DB - MTMT ER - TY - JOUR AU - Chen, K. AU - Song, J. AU - Song, Q. AU - Dou, X. AU - Wang, Y. AU - Wei, Y. AU - Chen, J. AU - Wang, L. AU - Alradi, M.F. AU - Liu, X. AU - Han, Z. AU - Feng, C. TI - Transcriptomic analysis provides insights into the immune responses and nutrition in Ostrinia furnacalis larvae parasitized by Macrocentrus cingulum JF - ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY J2 - ARCH INSECT BIOCHEM VL - 109 PY - 2022 IS - 3 PG - 20 SN - 0739-4462 DO - 10.1002/arch.21863 UR - https://m2.mtmt.hu/api/publication/32654955 ID - 32654955 N1 - Department of Plant Protection, College of Horticulture and Plant Protection, Yangzhou University, Jiangsu, Yangzhou, China Division of Plant Sciences, University of Missouri, Columbia, MO, United States Department of Entomology, University of Georgia, Athens, GA, United States Department of Biotechnology, Yangzhou University, Jiangsu, Yangzhou, China Export Date: 8 February 2022 CODEN: AIBPE Correspondence Address: Feng, C.; Department of Plant Protection, Jiangsu, China; email: fengcj@yzu.edu.cn AB - Macrocentrus cingulum is a principal endoparasite of Ostrinia furnacalis larvae. M. cingulum larvae repress host immune responses for survival and ingest host nutrients for development until emerging. However, most investigations focused on the mechanisms of how wasps repress the host immunity, the triggered immune responses and nutrient status altered by wasps in host are neglected. In this study, we found that parasitized O. furnacalis larvae activated fast recognition responses and produced some effectors such as lysozyme and antimicrobial peptides, along with more consumption of trehalose, glucose, and even lipid to defend against the invading M. cingulum. However, the expression of peroxidase 6 and superoxide dismutase 2 (SOD 2) was upregulated, and the messenger RNA (mRNA) levels of cellular immunity-related genes such as thioester-containing protein 2 (TEP 2) and hemocytin were also reduced, suggesting that some immune responses were selectively shut down by wasp parasitization. Taken together, all the results indicated that parasitized O. furnacalis larvae selectively activate the immune recognition response, and upregulate effector genes, but suppress ROS reaction and cellular immunity, and invest more energy to fuel certain immune responses to defend against the wasp invading. This study provides useful information for further identifying key components of the nutrition and innate immune repertoire which may shape host-parasitoid coevolutionary dynamics. © 2021 Wiley Periodicals LLC LA - English DB - MTMT ER - TY - JOUR AU - Luz, G.F.D. AU - Santana, W.C. AU - Santos, C.G. AU - Medeiros, Santana L. AU - Serrão, J.E. TI - Cuticle melanization and the expression of immune-related genes in the honeybee Apis mellifera (Hymenoptera: Apidae) adult workers JF - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY J2 - COMP BIOCHEM PHYS B VL - 257 PY - 2022 SN - 1096-4959 DO - 10.1016/j.cbpb.2021.110679 UR - https://m2.mtmt.hu/api/publication/32654950 ID - 32654950 N1 - Departamento de Biologia Geral/BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-000, Brazil Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, 36570-000, Brazil Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Rio Paranaíba, 38810-000, Brazil Export Date: 8 February 2022 CODEN: CBPBB Correspondence Address: Serrão, J.E.; Departamento de Biologia Geral, Brazil; email: jeserrao@ufv.br AB - The global decline of bee populations has several factors, including pathogens, which need overcome the insect defenses such as the physical barriers, the body cuticle and peritrophic matrix (primary defenses), as well as the secondary defenses with antimicrobial peptides (AMPs) and the enzyme lysozyme. The regulation of immune defenses according to the infection risks raises questions about the immunity of social bees due to their exposition to different pathogens pressures during the adult lifespan and tasks performed. This study evaluated the primary (body cuticle melanization, peritrophic matrix and cpr14 expression) and secondary (AMPs and lysozyme expression) defenses of the honeybee Apis mellifera workers according to the age and tasks. The expression of malvolio was used to detect precocious forage tasks outside the colony. Forager workers have higher amount of cuticular melanization in the body cuticle than nurse, but not when the age effect is retired, indicating the gradual acquisition of this compound in the integument of adult bees. The relative value of chitin in the peritrophic matrix and cpr14 mRNA are similar in all bees evaluated, suggesting that these components of primary defenses do not change according to the task and age. Differential expression of genes for AMPs in workers performing different tasks, within the same age group, indicates that the behavior stimulates expression of genes related to secondary immune defense. The expression of malvolio gene, accelerating the change in workers behavior, and those related to immune defense suggest the investment in secondary defense mechanisms when the primary defense of the body cuticle is not yet completed. © 2021 Elsevier Inc. LA - English DB - MTMT ER - TY - JOUR AU - Petronio, Giulio Petronio AU - Pietrangelo, Laura AU - Cutuli, Marco Alfio AU - Magnifico, Irene AU - Venditti, Noemi AU - Guarnieri, Antonio AU - Abate, Getnet Atinafu AU - Yewhalaw, Delenasaw AU - Davinelli, Sergio AU - Di, Marco Roberto TI - Emerging Evidence on Tenebrio molitor Immunity: A Focus on Gene Expression Involved in Microbial Infection for Host-Pathogen Interaction Studies JF - MICROORGANISMS J2 - MICROORGANISMS VL - 10 PY - 2022 IS - 10 PG - 24 SN - 2076-2607 DO - 10.3390/microorganisms10101983 UR - https://m2.mtmt.hu/api/publication/33260649 ID - 33260649 N1 - Export Date: 13 January 2023 AB - In recent years, the scientific community's interest in T. molitor as an insect model to investigate immunity and host-pathogen interactions has considerably increased. The reasons for this growing interest could be explained by the peculiar features of this beetle, which offers various advantages compared to other invertebrates models commonly used in laboratory studies. Thus, this review aimed at providing a broad view of the T. molitor immune system in light of the new scientific evidence on the developmental/tissue-specific gene expression studies related to microbial infection. In addition to the well-known cellular component and humoral response process, several studies investigating the factors associated with T. molitor immune response or deepening of those already known have been reported. However, various aspects remain still less understood, namely the possible crosstalk between the immune deficiency protein and Toll pathways and the role exerted by T. molitor apolipoprotein III in the expression of the antimicrobial peptides. Therefore, further research is required for T. molitor to be recommended as an alternative insect model for pathogen-host interaction and immunity studies. LA - English DB - MTMT ER - TY - JOUR AU - Dijokaite, Aiste AU - Humbert, Maria Victoria AU - Borkowski, Emma AU - La Ragione, Roberto M. AU - Christodoulides, Myron TI - Establishing an invertebrate Galleria mellonella greater wax moth larval model of Neisseria gonorrhoeae infection JF - VIRULENCE J2 - VIRULENCE VL - 12 PY - 2021 IS - 1 SP - 1900 EP - 1920 PG - 21 SN - 2150-5594 DO - 10.1080/21505594.2021.1950269 UR - https://m2.mtmt.hu/api/publication/32424526 ID - 32424526 AB - Neisseria gonorrhoeae (gonococcus) causes the human sexually transmitted disease gonorrhea. Studying gonococcal pathogenesis and developing new vaccines and therapies to combat the increasing prevalence of multi-antibiotic resistant bacteria has made use of many ex vivo models based on human cells and tissues, and in vivo vertebrate models, for example, rodent, pig and human. The focus of the current study was to examine the utility of the invertebrate greater wax moth Galleria mellonella as an in vivo model of gonococcal infection. We observed that a threshold of similar to 10(6) similar to 10(7) gonococci/larva was required to kill >50% of larvae (P < 0.05), and increased toxicity correlated with reduced health index scores and pronounced histopathological changes such as increases in the total lesion grade, melanized nodules, hemocyte reaction, and multifocal adipose body degeneration. Larval death was independent of the expression of pilus or Opa protein or LOS sialylation within a single gonococcal species studied, but the model could demonstrate relative toxicity of different isolates. N. meningitidis, N. lacatamica and gonococci all killed larvae equally, but were significantly less toxic (P > 0.05) than Pseudomonas aeruginosa. Larvae primed with nontoxic doses of gonococci were more susceptible to subsequent challenge with homologous and heterologous bacteria, and larval survival was significantly reduced (P < 0.05) in infected larvae after depletion of their hemocytes with clodronate-liposomes. The model was used to test the anti-gonococcal properties of antibiotics and novel antimicrobials. Ceftriaxone (P < 0.05) protected larvae from infection with different gonococcal isolates, but not azithromycin or monocaprin or ligand-coated silver nanoclusters (P > 0.05). LA - English DB - MTMT ER - TY - JOUR AU - Kaya, S. AU - Akkuş, G. AU - Türkdoğan, S. AU - Gündüz, B. TI - Influence of Helichrysum arenarium on hemocyte-mediated immune responses and phenoloxidase enzyme activity of model organism Galleria mellonella (L.) JF - INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE J2 - INT J TROP INSECT SC VL - 41 PY - 2021 IS - 4 SP - 2521 EP - 2528 PG - 8 SN - 1742-7584 DO - 10.1007/s42690-021-00432-8 UR - https://m2.mtmt.hu/api/publication/31872771 ID - 31872771 AB - Helichrysum arenarium (L.) (Asterales:Asteraceae) Moench is a therapeutic plant which contains etheric oil, flavones and flavon glycosides, sterins, bitter substances and tannins having various coumarins. This plant is thought to have important characteristics such as diuretic effect, dropping stones and sand from the kidney, regulating digestive disorders, strengthening the immune system, and having antibiotic and antioxidant effects. Additionally, this plant is traditionally used in liver and biliary tract diseases and also shows anti-inflammatory and detoxifying properties. Model organism Galleria mellonella L. (Lepidoptera:Pyralidae) is an invertebrate species that is frequently used to study the effects of human pathogens and various pesticides, hormones, etc. on immune system. In our study, we examined the effect of various doses of H. arenarium on the hemocyte count and behavior of G. mellonella larvae. According to the findings obtained at the end of our study, H. arenarium caused an increase in hemocyte count with the injection of 0.25% and 0.5% doses compared to the untreated and DMSO groups. At the same time, the 0.25% and 0.5% doses showed a strong encapsulation-melanization response and an increase in phenoloxidase enzyme activity over 24 h compared to the other injected groups. Based on these results, H. arenarium extract has an anti-mitotic activity at high doses (above 0.5%). This effect may be due to the fact that the plant extract supports mitosis at a certain dose, while being toxic when exceeding it. © 2021, African Association of Insect Scientists. LA - English DB - MTMT ER - TY - JOUR AU - Lima, Leticia Ferreira AU - Torres, Andre Quintanilha AU - Jardim, Rodrigo AU - Mesquita, Rafael Dias AU - Schama, Renata TI - Evolution of Toll, Spatzle and MyD88 in insects: the problem of the Diptera bias JF - BMC GENOMICS J2 - BMC GENOMICS VL - 22 PY - 2021 IS - 1 PG - 21 SN - 1471-2164 DO - 10.1186/s12864-021-07886-7 UR - https://m2.mtmt.hu/api/publication/32424527 ID - 32424527 AB - Background Arthropoda, the most numerous and diverse metazoan phylum, has species in many habitats where they encounter various microorganisms and, as a result, mechanisms for pathogen recognition and elimination have evolved. The Toll pathway, involved in the innate immune system, was first described as part of the developmental pathway for dorsal-ventral differentiation in Drosophila. Its later discovery in vertebrates suggested that this system was extremely conserved. However, there is variation in presence/absence, copy number and sequence divergence in various genes along the pathway. As most studies have only focused on Diptera, for a comprehensive and accurate homology-based approach it is important to understand gene function in a number of different species and, in a group as diverse as insects, the use of species belonging to different taxonomic groups is essential. Results We evaluated the diversity of Toll pathway gene families in 39 Arthropod genomes, encompassing 13 different Insect Orders. Through computational methods, we shed some light into the evolution and functional annotation of protein families involved in the Toll pathway innate immune response. Our data indicates that: 1) intracellular proteins of the Toll pathway show mostly species-specific expansions; 2) the different Toll subfamilies seem to have distinct evolutionary backgrounds; 3) patterns of gene expansion observed in the Toll phylogenetic tree indicate that homology based methods of functional inference might not be accurate for some subfamilies; 4) Spatzle subfamilies are highly divergent and also pose a problem for homology based inference; 5) Spatzle subfamilies should not be analyzed together in the same phylogenetic framework; 6) network analyses seem to be a good first step in inferring functional groups in these cases. We specifically show that understanding Drosophila's Toll functions might not indicate the same function in other species. Conclusions Our results show the importance of using species representing the different orders to better understand insect gene content, origin and evolution. More specifically, in intracellular Toll pathway gene families the presence of orthologues has important implications for homology based functional inference. Also, the different evolutionary backgrounds of Toll gene subfamilies should be taken into consideration when functional studies are performed, especially for TOLL9, TOLL, TOLL2_7, and the new TOLL10 clade. The presence of Diptera specific clades or the ones lacking Diptera species show the importance of overcoming the Diptera bias when performing functional characterization of Toll pathways. LA - English DB - MTMT ER - TY - JOUR AU - Mil-Homens, D. AU - Martins, M. AU - Barbosa, J. AU - Serafim, G. AU - Sarmento, M.J. AU - Pires, R.F. AU - Rodrigues, V. AU - Bonifácio, V.D.B. AU - Pinto, S.N. TI - Carbapenem-resistant klebsiella pneumoniae clinical isolates: In vivo virulence assessment in galleria mellonella and potential therapeutics by polycationic oligoethyleneimine JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 10 PY - 2021 IS - 1 PG - 14 SN - 2079-6382 DO - 10.3390/antibiotics10010056 UR - https://m2.mtmt.hu/api/publication/31872772 ID - 31872772 N1 - iBB—Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, Lisboa, 1049-001, Portugal J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Dolejskova 3, Prague, 18223, Czech Republic Secção de Microbiologia, Laboratório SYNLAB-Lisboa, Grupo SYNLAB Portugal, Av. Columbano Bordalo Pinheiro, 75 A, 2◦Andar, Lisboa, 1070-061, Portugal Export Date: 15 February 2021 Correspondence Address: Bonifácio, V.D.B.; iBB—Institute for Bioengineering and Biosciences, Av. Rovisco Pais, Portugal; email: vasco.bonifacio@tecnico.ulisboa.pt Correspondence Address: Pinto, S.N.; iBB—Institute for Bioengineering and Biosciences, Av. Rovisco Pais, Portugal; email: sandrapinto@ist.utl.pt AB - Klebsiella pneumoniae, one of the most common pathogens found in hospital-acquired infections, is often resistant to multiple antibiotics. In fact, multidrug-resistant (MDR) K. pneumoniae producing KPC or OXA-48-like carbapenemases are recognized as a serious global health threat. In this sense, we evaluated the virulence of K. pneumoniae KPC(+) or OXA-48(+) aiming at potential antimicrobial therapeutics. K. pneumoniae carbapenemase (KPC) and the expanded-spectrum oxacillinase OXA-48 isolates were obtained from patients treated in medical care units in Lisbon, Portugal. The virulence potential of the K. pneumonia clinical isolates was tested using the Galleria mellonella model. For that, G. mellonella larvae were inoculated using patients KPC(+) and OXA-48(+) isolates. Using this in vivo model, the KPC(+) K. pneumoniae isolates showed to be, on average, more virulent than OXA-48(+). Virulence was found attenuated when a low bacterial inoculum (one magnitude lower) was tested. In addition, we also report the use of a synthetic polycationic oligomer (L-OEI-h) as a potential antimicrobial agent to fight infectious diseases caused by MDR bacteria. L-OEI-h has a broad-spectrum antibacterial activity and exerts a significantly bactericidal activity within the first 5-30 min treatment, causing lysis of the cytoplasmic membrane. Importantly, the polycationic oligomer showed low toxicity against in vitro models and no visible cytotoxicity (measured by survival and health index) was noted on the in vivo model (G. mellonella), thus L-OEI-h is foreseen as a promising polymer therapeutic for the treatment of MDR K. pneumoniae infections. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Straw, E.A. AU - Brown, M.J.F. TI - Co-formulant in a commercial fungicide product causes lethal and sub-lethal effects in bumble bees JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 11 PY - 2021 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-021-00919-x UR - https://m2.mtmt.hu/api/publication/32654954 ID - 32654954 N1 - Cited By :1 Export Date: 8 February 2022 Correspondence Address: Straw, E.A.; Centre for Ecology, United Kingdom; email: EdwardAStraw@gmail.com AB - Pollinators, particularly wild bees, are suffering declines across the globe, and pesticides are thought to be drivers of these declines. Research into, and regulation of pesticides has focused on the active ingredients, and their impact on bee health. In contrast, the additional components in pesticide formulations have been overlooked as potential threats. By testing an acute oral dose of the fungicide product Amistar, and equivalent doses of each individual co-formulant, we were able to measure the toxicity of the formulation and identify the ingredient responsible. We found that a co-formulant, alcohol ethoxylates, caused a range of damage to bumble bee health. Exposure to alcohol ethoxylates caused 30% mortality and a range of sublethal effects. Alcohol ethoxylates treated bees consumed half as much sucrose as negative control bees over the course of the experiment and lost weight. Alcohol ethoxylates treated bees had significant melanisation of their midguts, evidence of gut damage. We suggest that this gut damage explains the reduction in appetite, weight loss and mortality, with bees dying from energy depletion. Our results demonstrate that sublethal impacts of pesticide formulations need to be considered during regulatory consideration, and that co-formulants can be more toxic than active ingredients. © 2021, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Xu, Junhuan AU - Willman, Matthew AU - Todd, Jane AU - Kim, Kwang-Ho AU - Redinbaugh, Margaret G. AU - Stewart, Lucy R. TI - Transcriptome of the Maize Leafhopper (Dalbulus maidis) and Its Transcriptional Response to Maize Rayado Fino Virus (MRFV), Which It Transmits in a Persistent, Propagative Manner JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 9 PY - 2021 IS - 3 PG - 20 SN - 2165-0497 DO - 10.1128/Spectrum.00612-21 UR - https://m2.mtmt.hu/api/publication/32970995 ID - 32970995 N1 - Department of Plant Pathology, Ohio State University, Wooster, OH, United States USDA-ARS Corn, Soybean and Wheat Quality Research Unit, Wooster, OH, United States National Institute of Agricultural Sciences, Crop Protection Division 166, Wanju-gun, Jeollabuk-do, South Korea USDA-ARS, Foreign Disease-Weed Science Research Unit, Frederick, MD, United States Export Date: 8 February 2022 Correspondence Address: Stewart, L.R.; USDA-ARS, United States; email: lucy.stewart@usda.gov AB - The corn leafhopper (Dalbulus maidis) is an important vector of maize rayado fino virus (MRFV), a positive-strand RNA (+ssRNA) marafivirus which it transmits in a persistent propagative manner. The interaction of D. maidis with MRFV, including infection of the insect and subsequent transmission to new plants, is not well understood at the molecular level. To examine the leafhopper-virus interaction, a D. maidis transcriptome was assembled and differences in transcript abundance between virus-exposed and naive D. maidis were examined at two time points (4 h and 7 days) post exposure to MRFV. The D. maidis transcriptome contained 56,116 transcripts generated from 1,727,369,026 100-nt paired-end reads from whole adult insects. The transcriptome of D. maidis shared highest identity and most orthologs with the leafhopper Graminella nigrifrons (65% of transcripts had matches with E values of <10-5) versus planthoppers Sogatella furcifera (with 23% of transcript matches below the E value cutoff) and Peregrinus maidis (with 21% transcript matches below the E value cutoff), as expected based on taxonomy. D. maidis expressed genes in the Toll, Imd, and Jak/Stat insect immune signaling pathways, RNA interference (RNAi) pathway genes, prophenoloxidase-activating system pathways, and immune recognition protein-encoding genes such as peptidoglycan recognition proteins (PGRPs), antimicrobial peptides, and other effectors. Statistical analysis (performed by R package DESeq2) identified 72 transcripts at 4 h and 67 at 7 days that were significantly responsive to MRFV exposure. Genes expected to be favorable for virus propagation, such as protein synthesis-related genes and genes encoding superoxide dismutase, were significantly upregulated after MRFV exposure.IMPORTANCE The transcriptome of the corn leafhopper, D. maidis, revealed conserved biochemical pathways for immunity and discovered transcripts responsive to MRFV-infected plants at two time points, providing a basis for functional identification of genes that either limit or promote the virus-vector interaction. Compared to other hopper species and the propagative plant viruses they transmit, D. maidis shared 15 responsive transcripts with S. furcifera (to southern rice black-streaked dwarf virus [SRBSDV]), one with G. nigrifrons (to maize fine streak virus [MFSV]), and one with P. maidis (to maize mosaic virus [MMV]), but no virus-responsive transcripts identified were shared among all four hopper vector species. LA - English DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Rusvai, Miklós AU - Honti, Viktor AU - Kolics, Balázs AU - Török, Tibor AU - Williams, Michael J AU - Kurucz, Judit Éva AU - Andó, István TI - Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 109 PY - 2020 SP - 103701 PG - 5 SN - 0145-305X DO - 10.1016/j.dci.2020.103701 UR - https://m2.mtmt.hu/api/publication/31302837 ID - 31302837 N1 - The publication is supported by the EFOP-3.6.3-VEKOP-16-2017-00008 project. The project is co-financed by the European Union and the European Social Fund. Immunology Unit, Institute of Genetics, Biological Research Centre, P.O.Box 521, Szeged, H-6701, Hungary University of Veterinary Medicine, István u. 2., Budapest, 1078, Hungary Department of Plant Science and Biotechnology, University of Pannonia, Georgikon Faculty, Deák F. u. 16., Keszthely, 8360, Hungary Department of Genetics, University of Szeged, Közép Fasor 52, Szeged, 6726, Hungary Functional Pharmacology, Department of Neuroscience, Uppsala University, Husargatan 3, Box 593, Uppsala, 751 24, Sweden Export Date: 10 December 2020 CODEN: DCIMD Correspondence Address: Andó, I.; Immunology Unit, Institute of Genetics, Biological Research Centre, P.O.Box 521, Hungary; email: ando@brc.hu AB - Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes. LA - English DB - MTMT ER - TY - JOUR AU - Kaya, Serhat AU - Uckan, Fevzi AU - Er, Aylin TI - Influence of Indole-3-Acetic Acid on Cellular Immune Responses ofGalleria mellonellaL. (Lepidoptera: Pyralidae) andPimpla turionellaeL. (Hymenoptera: Ichneumonidae) in a host-parasitoid system JF - INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE J2 - INT J TROP INSECT SC PY - 2020 PG - 11 SN - 1742-7584 DO - 10.1007/s42690-020-00190-z UR - https://m2.mtmt.hu/api/publication/31467643 ID - 31467643 N1 - Cited By :1 Export Date: 10 May 2021 Correspondence Address: Kaya, S.; Faculty of Arts and Science, Turkey; email: serhatkaya@comu.edu.tr AB - The dietary effects of the well-known auxin member plant growth regulator, indole-3-acetic acid (IAA), on hemocyte counts and behavior of the model insectGalleria mellonellaL. (Lepidoptera: Pyralidae) and its parasitoidPimpla turionellaeL. (Hymenoptera: Ichneumonidae) were examined.In vivoandin vitroexperiments involved five treatments applied to larvae with doses of 50, 500, 1000, 5000, and 10000 ppm IAA and the controls. Total hemocyte counts increased at all applied IAA doses inG. mellonellawhereas no effect was observed inP. turionellae. According to acridine orange-ethidium bromide double staining, none of the IAA doses affected the ratio of apoptotic and mitotic cellsin vivohowever a decrease in the number of late apoptotic cells at 1000 and 5000 ppm was observed atin vitroexperiments inG. mellonella. An increase in the percentage of early apoptotic hemocytes at 1000 ppm and late apoptotic hemocytes at 500 ppm were determined inP. turionellaelarvae. IAA also increased the ratio of mitotic hemocytes in the circulation ofP. turionellaelarvae at 50, 1000, and 10000 ppm. Changes in the encapsulation rate of Sephadex A-25 beads were not significant however the increase at 5000 ppm at 4th hour in non-melanized hemocytes was recorded as compared to the control. A strong decrease in melanization at 50 ppm and an increase at 50 and 1000 ppm in non-melanized bead count at 24 h were also observed. It can be concluded that IAA is inappropriate to use in combination with microbial control agents in IPM programs due to elevated hemocyte counts of the pest. LA - English DB - MTMT ER - TY - JOUR AU - Kikuchi, David W. AU - Waldron, Samuel J. AU - Valkonen, Janne K. AU - Dobler, Susanne AU - Mappes, Johanna TI - Biased predation could promote convergence yet maintain diversity within Mullerian mimicry rings of Oreina leaf beetles JF - JOURNAL OF EVOLUTIONARY BIOLOGY J2 - J EVOLUTION BIOL VL - 33 PY - 2020 IS - 7 SP - 887 EP - 898 PG - 12 SN - 1010-061X DO - 10.1111/jeb.13620 UR - https://m2.mtmt.hu/api/publication/31467647 ID - 31467647 AB - Mullerian mimicry is a classic example of adaptation, yet Muller's original theory does not account for the diversity often observed in mimicry rings. Here, we aimed to assess how well classical Mullerian mimicry can account for the colour polymorphism found in chemically defended Oreina leaf beetles by using field data and laboratory assays of predator behaviour. We also evaluated the hypothesis that thermoregulation can explain diversity between Oreina mimicry rings. We found that frequencies of each colour morph were positively correlated among species, a critical prediction of Mullerian mimicry. Predators learned to associate colour with chemical defences. Learned avoidance of the green morph of one species protected green morphs of another species. Avoidance of blue morphs was completely generalized to green morphs, but surprisingly, avoidance of green morphs was less generalized to blue morphs. This asymmetrical generalization should favour green morphs: indeed, green morphs persist in blue communities, whereas blue morphs are entirely excluded from green communities. We did not find a correlation between elevation and coloration, rejecting thermoregulation as an explanation for diversity between mimicry rings. Biased predation could explain within-community diversity in warning coloration, providing a solution to a long-standing puzzle. We propose testable hypotheses for why asymmetric generalization occurs, and how predators maintain the predominance of blue morphs in a community, despite asymmetric generalization. LA - English DB - MTMT ER - TY - JOUR AU - Ni, W. AU - Bao, J. AU - Mo, B. AU - Liu, L. AU - Li, T. AU - Pan, G. AU - Chen, J. AU - Zhou, Z. TI - Hemocytin facilitates host immune responses against Nosema bombycis JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 103 PY - 2020 SN - 0145-305X DO - 10.1016/j.dci.2019.103495 UR - https://m2.mtmt.hu/api/publication/31149578 ID - 31149578 N1 - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China Chongqing Normal University, Chongqing, China Export Date: 31 January 2020 CODEN: DCIMD Correspondence Address: Zhou, Z.; State Key Laboratory Of Silkworm Genome Biology, Southwest UniversityChina; email: zyzhou@swu.edu.cn State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China Chongqing Normal University, Chongqing, China Cited By :2 Export Date: 10 December 2020 CODEN: DCIMD Correspondence Address: Zhou, Z.; State Key Laboratory Of Silkworm Genome Biology, Southwest UniversityChina; email: zyzhou@swu.edu.cn AB - Invertebrates lack an adaptive immune response and thus are reliant on their innate immune response for eliminating invading pathogens. The innate immune responses of silkworms against the pathogen Nosema bombycis include: hemocyte aggregation, melanization, antimicrobial peptides, etc. In our current study, we discovered that a silkworm hemostasis-related protein, hemocytin, is up-regulated after Nosema bombycis infection. This novel finding lead to our hypothesis that hemocytin participates in immune responses against N. bombycis. We investigated this hypothesis by analyzing the adhesive effects of hemocytin to invading N. bombycis, and the hemocytin-mediated hemocyte aggregation and hemolymph melanization. We showed that hemocytin can adhere to the surface of N. bombycis, which facilitates the agglutination of N. bombycis and hemocytes as well as the subsequent melanization. Moreover, when we utilize RNAi technology to decrease in vivo hemocytin expression, we found that the proliferation of N. bombycis within the host significantly increased. These results support our hypothesis that hemocytin exerts pro-inflammatory effects by facilitating pathogen agglutination, along with hemocyte aggregation and melanization, to combat N. bombycis. Our study is the first to determine a function of hemocytin in innate immunity against N. bombycis. Moreover, our findings are of great importance to provide potential targets for developing novel strategy against microsporidia infection. © 2019 Elsevier Ltd LA - English DB - MTMT ER - TY - JOUR AU - Sheehan, Gerard AU - Farrell, Gemma AU - Kavanagh, Kevin TI - Immune priming: the secret weapon of the insect world JF - VIRULENCE J2 - VIRULENCE VL - 11 PY - 2020 IS - 1 SP - 238 EP - 246 PG - 9 SN - 2150-5594 DO - 10.1080/21505594.2020.1731137 UR - https://m2.mtmt.hu/api/publication/31467640 ID - 31467640 AB - Insects are a highly successful group of animals that inhabit almost every habitat and environment on Earth. Part of their success is due to a rapid and highly effective immune response that identifies, inactivates, and eliminates pathogens. Insects possess an immune system that shows many similarities to the innate immune system of vertebrates, but they do not possess an equivalent system to the antibody-mediated adaptive immune response of vertebrates. However, some insect do display a process known as immune priming in which prior exposure to a sublethal dose of a pathogen, or pathogen-derived material, leads to an elevation in the immune response rendering the insect resistant to a subsequent lethal infection a short time later. This process is mediated by an increase in the density of circulating hemocytes and increased production of antimicrobial peptides. Immune priming is an important survival strategy for certain insects while other insects that do not show this response may have colony-level behaviors that may serve to limit the success of pathogens. Insects are now widely used as in vivo models for studying microbial pathogens of humans and for assessing the in vivo efficacy of antimicrobial agents. Knowledge of the process of immune priming in insects is essential in these applications as it may operate and augment the perceived in vivo antimicrobial activity of novel compounds. LA - English DB - MTMT ER - TY - JOUR AU - Wang, Hai-Zhen AU - Zhong, Xin AU - Zhang, Gu-Ren AU - Liu, Xin AU - Gu, Li TI - Transcriptome characterization and gene expression analysis related to immune response in Gynaephora qinghaiensis pupae JF - JOURNAL OF ASIA PACIFIC ENTOMOLOGY J2 - J ASIA PAC ENTOMOL VL - 23 PY - 2020 IS - 2 SP - 458 EP - 469 PG - 12 SN - 1226-8615 DO - 10.1016/j.aspen.2020.01.013 UR - https://m2.mtmt.hu/api/publication/31467645 ID - 31467645 AB - Gynaephora qinghaiensis is a pest on the Qinghai-Tibet Plateau (QTP) that has led to substantial destruction of grassland vegetation. Its pupae are also natural hosts of parasitic wasp. Sexual dimorphism in immune responses is prevalent in vertebrates and invertebrates. However, sexual dimorphism in immune responses of insects, particularly G. qinghaiensis, is poorly understood at the transcriptional level. Here, we performed transcriptome sequencing in male and female pupae of G. qinghaiensis. A total of 118,357,040 clean reads were obtained and assembled into 114,944 unigenes. To explore the difference of immune responses in pupae of both sexes (male and female) of G. qinghaiensis. based on transcriptional level, we characterized the expression profiles of candidate transcripts in the two sexes of G. qinghaiensis. A total of 3,469 unigenes (1,888 up-regulated and 1,581 down-regulated genes) were differentially expressed in pupae of both sexes. Among the differentially expressed genes (DEGs), 263 unigenes related to immune responses were found in the two sexes in G. qinghaiensis; of these, 202 were up-regulated and 61 were down-regulated in the female pupae compared to male pupae. This indicates there were some differences in the expression of immune-related genes between male and female G. qinghaiensis pupae. Therefore, we speculated that the differences in immune responses also likely exsited in pupae of both sexes. Our report provides a valuable genomic resource for further studies of Gynaephora and improves our understanding of the molecular mechanisms underlying immunological differences between male and female insects. LA - English DB - MTMT ER - TY - JOUR AU - Wojda, I. AU - Staniec, B. AU - Sułek, M. AU - Kordaczuk, J. TI - The greater wax moth Galleria mellonella: Biology and use in immune studies JF - PATHOGENS AND DISEASE J2 - PATHOG DIS VL - 78 PY - 2020 IS - 9 SN - 2049-632X DO - 10.1093/femspd/ftaa057 UR - https://m2.mtmt.hu/api/publication/32006486 ID - 32006486 N1 - Maria Curie Sklodowska University, Institute of Biological Sciences, Department of Immunobiology, Akademicka 19, Lublin, 20-033, Poland Maria Curie Sklodowska University, Institute of Biological Sciences, Department of Zoology and Nature Protection, Akademicka 19, Lublin, 20-033, Poland Cited By :2 Export Date: 10 May 2021 Correspondence Address: Wojda, I.; Maria Curie Sklodowska University, Akademicka 19, Poland; email: wojda@poczta.umcs.lublin.pl AB - The greater wax moth Galleria mellonella is an invertebrate that is increasingly being used in scientific research. Its ease of reproduction, numerous offspring, short development cycle, and finally, its known genome and immune-related transcriptome provide a convenient research model for investigation of insect immunity at biochemical and molecular levels. Galleria immunity, consisting of only innate mechanisms, shows adaptive plasticity, which has recently become the subject of intensive scientific research. This insect serves as a mini host in studies of the pathogenicity of microorganisms and in vivo tests of the effectiveness of single virulence factors as well as new antimicrobial compounds. Certainly, the Galleria mellonella species deserves our attention and appreciation for its contribution to the development of research on innate immune mechanisms. In this review article, we describe the biology of the greater wax moth, summarise the main advantages of using it as a model organism and present some of the main techniques facilitating work with this insect © 2020 The Author(s) 2020. Published by Oxford University Press on behalf of FEMS. LA - English DB - MTMT ER - TY - JOUR AU - Yelkovan, Sedat AU - Arikan, Huseyin AU - Cakici, Ozlem TI - Caste and age-related changes in circulatory hemocytes of honey bee, Apis mellifera anatolica (Hymenoptera: Apidae) JF - JOURNAL OF APICULTURAL RESEARCH J2 - J APICULT RES PY - 2020 PG - 10 SN - 0021-8839 DO - 10.1080/00218839.2020.1834768 UR - https://m2.mtmt.hu/api/publication/31685791 ID - 31685791 N1 - Science Faculty, Biology Department, Zoology Section, Ege University, Izmir, Turkey Pilot University of Central Coordination Unit, Bingöl University, Bingol, Turkey Export Date: 10 May 2021 CODEN: JACRA Correspondence Address: Çakıcı, Ö.; Science Faculty, Turkey AB - In recent years, there has been a great concern about increased honey bee losses and this phenomenon has turned into a global issue. Therefore, immune system research on honey bees gained importance. Hemocytes are an important part of the immune system. But there is a limited study about hemocyte types of honey bees. From this point of view, a detailed study was performed on hemocyte types of honey bee Apis mellifera anatolica was described in larval (3rd, 4th and 5th larval instars), prepupal, pupal, and adult stages of all castes including worker, drone, and queen. According to light microscopic examinations, prohemocyte, plasmatocyte, granulocyte, adipohemocyte and oenocytoid were determined in all castes. Prohemocytes are usually rounded cells with a large nucleus and small cytoplasmic area. Plasmatocytes are spherical or oval cells. Granulocytes are generally spherical cells with dense granules. Adipohemocytes are usually spherical cells having numerous lipid droplets within their cytoplasm. Oenocytoides are usually spherical cells with small and spherical nucleus centrally located. Also, we statistically compared the size of hemocyte types among these castes during metamorphosis. According to our measurements, the queen generally had the biggest hemocytes in comparison to the developmental stages in other castes. While statistically significant differences in the sizes of hemocyte types were mainly detected among castes of the pupal stage, there were no statistically significant differences in hemocyte sizes among castes of adult honey bees. In view of these findings, it may be stated that hemocyte profiles of A. m. anatolica vary based on the developmental stages of castes. LA - English DB - MTMT ER - TY - JOUR AU - Al-Saeedi, H.M. AU - Al-Jassany, R.F. TI - Isolation and diagnostic Bacillus thuringiensis tenebrionis pathogenesis for insects from date palm stemborerlarva(JebusaeahammersChmidt:Coleoptera:Cearmbyicidae) JF - PLANT ARCHIVES J2 - PLANT ARCH VL - 19 PY - 2019 IS - 2 SP - 3331 EP - 3337 PG - 7 SN - 0972-5210 UR - https://m2.mtmt.hu/api/publication/31872761 ID - 31872761 N1 - Export Date: 15 February 2021 Correspondence Address: Al-Jassany, R.F.; Department of Plant Protection, Iraq; email: Radhialjassany@yahoo.com AB - The aim of this study to isolate and diagnose pathogen bacteria that caused date palm stem borer larva.economic important for date palm in Iraq. The process of diagnostic depend on morphological properties and biochemical tests in addition it biophysical specialties and polymerase chain reaction (PCR) for Cry3 gene, the pictures when taken by transmission electron microscope (TEM). The results of different tests when fourel this bacteria responsible for larvae death Bacillus thuringiensis subsp. tenebrionis. through compatibility of nucleotide based sequences in 99% between molecular diagnostic results and Cry gene analysis Bacillus thuringiensis serovar morrisoni BBSC 4AA1 which NCBI bank genes. The isolated bacteria B. t. tenebrionis caused 100% mortality after 2 day in the first and second larvae instare of date palm stem borer Jebusaea hammerschmidt and 100% mortality in the last instar of the larvae after 4 days.This bacteria B. t. tenebrionis was recorded the first time on larvae of date palm stem borer. © 2019 Plant Archives. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Chakraborty, Mousumi AU - Mandal, Santi M. AU - Basak, Amit AU - Ghosh, Ananta K. TI - Identification of a novel humoral antifungal defense molecule in the hemolymph of tasar silkworm Antheraea mylitta JF - BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS J2 - BIOCHEM BIOPH RES CO VL - 519 PY - 2019 IS - 1 SP - 121 EP - 126 PG - 6 SN - 0006-291X DO - 10.1016/j.bbrc.2019.08.143 UR - https://m2.mtmt.hu/api/publication/31580431 ID - 31580431 AB - Humoral defenses are the major components of insect innate immune system that include the production of several soluble effector molecules from fat body and hemocytes, and released in to the hemolymph upon microbial infection. Hemolymph was collected from the fungal immunized fifth instar larvae of tasar silkworm, Antheraea mylitta, extracted with a mixture of solvent (methanol/glacial acetic acid/water) and fractionated through RP-HPLC. Several fractions were collected, lyophilized and their antifungal activity was tested against Candida albicans. Only the fraction showing strong antifungal activity was further purified via gel filtration chromatography and the purity of active compound was confirmed by thin layer chromatography which showed only single spot after staining with ninhydrin. The molecular mass of this purified compound was determined by high resolution mass spectrometry as 531 Da and analysis of H-1 and C-13 NMR spectral data along with mass fragmentation pattern indicated the probable structure of the isolated compound as symmetric bis-decanoate derivative. Scanning electron microscopic study revealed that the compound degraded fungal cell wall leading to its lysis and may be the major target for its antifungal activity. These results indicate that presence of this compound in the hemolymph of A. mylitta provides defense against fungal infection. (C) 2019 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Cotter, Sheena C. AU - Reavey, Catherine E. AU - Tummala, Yamini AU - Randall, Joanna L. AU - Holdbrook, Robert AU - Ponton, Fleur AU - Simpson, Stephen J. AU - Smith, Judith A. AU - Wilson, Kenneth TI - Diet modulates the relationship between immune gene expression and functional immune responses JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 109 PY - 2019 SP - 128 EP - 141 PG - 14 SN - 0965-1748 DO - 10.1016/j.ibmb.2019.04.009 UR - https://m2.mtmt.hu/api/publication/30906927 ID - 30906927 AB - Nutrition is vital to health and the availability of resources has long been acknowledged as a key factor in the ability to fight off parasites, as investing in the immune system is costly. Resources have typically been considered as something of a "black box", with the quantity of available food being used as a proxy for resource limitation. However, food is a complex mixture of macro- and micronutrients, the precise balance of which determines an animal's fitness. Here we use a state-space modelling approach, the Geometric Framework for Nutrition (GFN), to assess for the first time, how the balance and amount of nutrients affects an animal's ability to mount an immune response to a pathogenic infection.Spodoptera littoralis caterpillars were assigned to one of 20 diets that varied in the ratio of macronutrients (protein and carbohydrate) and their calorie content to cover a large region of nutrient space. Caterpillars were then handled or injected with either live or dead Xenorhabdus nematophila bacterial cells. The expression of nine genes (5 immune, 4 non-immune) was measured 20 h post immune challenge. For two of the immune genes (PPO and Lysozyme) we also measured the relevant functional immune response in the hemolymph. Gene expression and functional immune responses were then mapped against nutritional intake.The expression of all immune genes was up-regulated by injection with dead bacteria, but only those in the IMD pathway (Moricin and Relish) were substantially up-regulated by both dead and live bacterial challenge. Functional immune responses increased with the protein content of the diet but the expression of immune genes was much less predictable.Our results indicate that diet does play an important role in the ability of an animal to mount an adequate immune response, with the availability of protein being the most important predictor of the functional (physiological) immune response. Importantly, however, immune gene expression responds quite differently to functional immunity and we would caution against using gene expression as a proxy for immune investment, as it is unlikely to be reliable indicator of the immune response, except under specific dietary conditions. LA - English DB - MTMT ER - TY - JOUR AU - El Sheikha, Aly Farag AU - Menozzi, Philippe TI - Potential geo-tracing tool for migrant insects by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE JF - INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE J2 - INT J TROP INSECT SC VL - 39 PY - 2019 IS - 1 SP - 9 EP - 16 PG - 8 SN - 1742-7584 DO - 10.1007/s42690-019-00002-z UR - https://m2.mtmt.hu/api/publication/30906926 ID - 30906926 AB - Insect movement in the landscape remains often poorly known and in some cases does not make it possible to understand the role of the different cultivated and wild habitats in the dynamics of useful and pest insects. Insects are among the greatest pests of agriculture, horticulture and forestry worldwide, inflicting damage and economic costs both directly and by transmitting plant viruses. There is a need for tracking the migrant insects in agroecosystems through space and time to establish their migation. Furthermore, tracing the origin of pest insects allows to design more rapid, efficient and environment-friendly control systems (less use of insecticides). Tracking insects can also help us better understanding their biology (e.g. insect population dynamics, geo-traceability, feeding behavior and other ecological interactions). However. tracking insects presents a considerable challenge as they are often small, cryptic and highly mobile organisms. The most common methods of analysis currently proposed (microsatellite markers, stable isotopes) do not allow for the moment to determine their geographical origin. The ecological niche occupied by the insects highly influences the bacterial communities associated with the insects. Hence understanding the diversity of bacterial communities could be an option to trace the orgins of the pest. This paper presents two new protocols used to extract bacterial DNA from the Cotton Bollworm. Helicoverpa armigera and the analysis of this DNA by PCR-DGGE. This promising method is proposed as a new traceability tool which provides insects with a unique biological barcode and makes it possible to trace back the insects to their original location. LA - English DB - MTMT ER - TY - CHAP AU - Istkhar, . AU - Chaubey, A.K. AU - Garg, A.P. TI - Entomopathogenic nematodes in the biological control of insect pests with reference to insect immunity T2 - Plant Biotic Interactions: State of the Art PB - Springer Netherlands SN - 9783030266578 PY - 2019 SP - 181 EP - 209 PG - 29 DO - 10.1007/978-3-030-26657-8_11 UR - https://m2.mtmt.hu/api/publication/31872769 ID - 31872769 N1 - Export Date: 15 February 2021 Correspondence Address: Istkhar; Department of Bioscience and Biotechnology, India AB - Insects are the successfully living arthropods on earth and challenging humans for food and shelter from several decades. As agricultural pests, insects cause heavy losses to crops and reduce the quality and quantity of human food. Failure of chemical pesticides and development of resistance of insects have become the major problem for researchers and are challenging mankind to search out for better solutions. Biological control management has been found as an emerging key of success, and in this context, entomopathogenic nematodes are in concern as potential biological control agents. These nematodes parasitize and enter the body of insect host and kill them quickly without the development of any resistance and failure. Several species of the entomopathogenic nematode genera Steinernema and Heterorhabditis have been identified around the world, and few of them have been commercialized for the biomanagement of several insect pests. The killing capabilities of these nematodes were enhanced severalfold by their symbiont bacteria of the genera Xenorhabdus and Photorhabdus which reside in the alimentary canal of these rhabditid nematodes. The symbiont bacteria produce severe toxins which ultimately results in the death of insect hosts. For the successful establishment of a biological species as biocontrol agent of insects, it is necessary to study the immune responses of host and defensive mechanisms of the parasites. Insects comprise innate immune system where cellular and humoral components combat with the invading pathogens. When entomopathogenic nematodes enter in the haemocoel of insect’s body, both the immune components are triggered and try to evade pathogenicity of invading nematode-bacterium complexes. The mechanisms of nematode immunomodulation display the variation of modulatory approaches developed by different types of parasitic nematodes to cripple the host immune responses. Both the nematode and bacteria produce chemical factors which protect them from the insect’s immune system attack. The cellular and humoral responses are avoided and broken by toxins produced by nematode-bacterium complexes included different proteins producing immunodeficiency in insects. These nematodes were found to be safe to the environments and soil flora and fauna and also have exemption from one area to another by several countries. © Springer Nature Switzerland AG 2019. LA - English DB - MTMT ER - TY - JOUR AU - Kamal, F. AU - Peters, D.L. AU - McCutcheon, J.G. AU - Dunphy, G.B. AU - Dennis, J.J. TI - Use of Greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for Analysis of Bacterial Pathogenesis JF - METHODS IN MOLECULAR BIOLOGY J2 - METHODS MOL BIOL VL - 1898 PY - 2019 SP - 163 EP - 171 PG - 9 SN - 1064-3745 DO - 10.1007/978-1-4939-8940-9_13 UR - https://m2.mtmt.hu/api/publication/31872770 ID - 31872770 N1 - Faculty of Science, Biological Sciences, University of Alberta, Edmonton, AB, Canada Natural Resource Sciences, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, QC, Canada Cited By :2 Export Date: 15 February 2021 Correspondence Address: Dennis, J.J.; Faculty of Science, Canada; email: jon.dennis@ualberta.ca AB - Alternative infection models of bacterial pathogenesis are useful because they reproduce some of the disease characteristics observed in higher animals. Insect models are especially useful for modeling bacterial infections, as they are inexpensive, generally less labor-intensive, and more ethically acceptable than experimentation on higher organisms. Similar to animals, insects have been shown to possess innate immune systems that respond to pathogenic bacteria. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. LA - English DB - MTMT ER - TY - JOUR AU - Lecocq, Antoine AU - Jensen, Annette Bruun AU - Eilenberg, Jurgen TI - Diseases of insects in European production systems: Diagnosis, prevention and management JF - BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT J2 - BERL MUNCH TIERARZTL VL - 132 PY - 2019 IS - 5-6 SP - 245 EP - 250 PG - 6 SN - 0005-9366 DO - 10.2376/0005-9366-18061 UR - https://m2.mtmt.hu/api/publication/30906929 ID - 30906929 AB - The rapid growth of insect production for food and feed in Europe in the 21st century is accompanied by a need for better tools to assist producers in the diagnosis, prevention and management of insect diseases, which may threaten productivity. In this paper we introduce the main pathogens (viruses, bacteria, fungi) associated with insect production in European facilities as well as their diagnosis. Furthermore, we provide an introduction to insect immunity and we give guidelines for the prevention and management of diseases in order to minimize risk and losses to the producers. We describe our ongoing research on probiotics as a tool to enhance insect health. More European collaborations on diagnosis and management of insect diseases are needed, and our team welcomes insect producers, veterinarians, food microbiologists etc. to develop such collaboration. LA - English DB - MTMT ER - TY - JOUR AU - Trevijano-Contador, Nuria AU - Zaragoza, Oscar TI - Immune Response of Galleria mellonella against Human Fungal Pathogens JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 5 PY - 2019 IS - 1 PG - 13 SN - 2309-608X DO - 10.3390/jof5010003 UR - https://m2.mtmt.hu/api/publication/30906930 ID - 30906930 AB - In many aspects, the immune response against pathogens in insects is similar to the innate immunity in mammals. This has caused a strong interest in the scientific community for the use of this model in research of host-pathogen interactions. In recent years, the use of Galleria mellonella larvae, an insect belonging to the Lepidoptera order, has emerged as an excellent model to study the virulence of human pathogens. It is a model that offers many advantages; for example, it is easy to handle and establish in every laboratory, the larvae have a low cost, and they tolerate a wide range of temperatures, including human temperature 37 degrees C. The immune response of G. mellonella is innate and is divided into a cellular component (hemocytes) and humoral component (antimicrobial peptides, lytic enzymes, and peptides and melanin) that work together against different intruders. It has been shown that the immune response of this insect has a great specificity and has the ability to distinguish between different classes of microorganisms. In this review, we delve into the different components of the innate immune response of Galleria mellonella, and how these components manifest in the infection of fungal pathogens including Candida albicans, Aspergillus fumigatus, Cryptococcus neoformans, and Histoplasma capsulatum. LA - English DB - MTMT ER - TY - JOUR AU - Turgay-Izzetoglu, Gamze AU - Gulmez, Mehtap TI - Characterization of fat body cells at different developmental stages of Culex pipiens JF - ACTA HISTOCHEMICA J2 - ACTA HISTOCHEM VL - 121 PY - 2019 IS - 4 SP - 460 EP - 471 PG - 12 SN - 0065-1281 DO - 10.1016/j.acthis.2019.04.002 UR - https://m2.mtmt.hu/api/publication/30906928 ID - 30906928 AB - The fat body, originates from mesoderm, has many metabolic functions which changes as the embryonic development of the insect progresses. It plays an important role in the intermediate metabolism and in the metabolism of proteins, lipids and carbohydrates. It has roles in synthesis, absorption and storage of nutrients from hemolymph. It is also responsible for the production of immunological system components, antibacterial compounds and blood clotting proteins. The most common type of fat body cells are trophocytes (the basic cells of the fat body) and oenocytes are found associated with the fat body. In this study, it is aimed at determining the cell types contained in the fat body of Culex pipiens at different developmental stages as well as identifying the molecules such as carbohydrate, protein and lipid contained in each of these cells. Knowing the regional distribution of the fat body cells and the concentration of its content at each developmental stage is important in understanding the process related to its physiology and it may help in fighting against the pest C. pipiens, which is a vector species for many contagious diseases observed in humans and other species.To achieve our goal, we have employed different histochemical techniques (fixatives and staining methods) for staining C. pipiens preparates of different developmental stages and analyzed the structure of the fat body, its distribution, its cell types and the macromolecular contents of the cells. We only observed trophocytes and oenocytes as fat body components in C. pipiens. The trophocytes had all the three macromolecules (lipids, proteins, carbohydrates) in the cytoplasm varying in concentration between the different regions and different stages. The oenocytes were observed below the integument as well as between the muscles in the larvae of Culex pipiens. They were present either as single cells or in clusters and also varied in size. Their cytoplasm was stained strongly for proteins when bromophenol blue staining was applied, but it was rather heterogeneous due to the lipid inclusions. On the contrary, oenocytes were not observed among the adult C. pipiens preparations. LA - English DB - MTMT ER - TY - JOUR AU - Vertyporokh, Lidiia AU - Kordaczuk, Jakub AU - Mak, Pawel AU - Hulas-Stasiak, Monika AU - Wojda, Iwona TI - Host-pathogen interactions upon the first and subsequent infection of Galleria mellonella with Candida albicans JF - JOURNAL OF INSECT PHYSIOLOGY J2 - J INSECT PHYSIOL VL - 117 PY - 2019 PG - 14 SN - 0022-1910 DO - 10.1016/j.jinsphys.2019.103903 UR - https://m2.mtmt.hu/api/publication/30906925 ID - 30906925 AB - Insects are able to develop enhanced resistance in response to repeated infection. This phenomenon is called immune priming. In this work, so-called "primed" Galleria mellonella larvae were re-infected with a lethal dose of Candida albicans 48 h after injection of a non-lethal dose, while "non-primed" larvae were infected only with a lethal dose. The increased resistance of the primed larvae correlated with a slower rate of body colonisation by the fungus. Changes in the protein profiles were detected in the whole hemolymph of the primed insects. The analysis of low-molecular weight proteins and peptides obtained with the use of three different organic solvents and comparative quantitative HPLC analysis thereof showed that the primed larvae did not have higher amounts of any infection-inducible polypeptides than the non-primed larvae. Moreover, electrophoresis of low-molecular weight polypeptides revealed an even lower level of immune-induced peptides in the primed larvae than in the non-primed ones.Furthermore, the defence activity of larval hemolymph, i.e. the antifungal, antibacterial, and lysozyme-type activity, was up-regulated in the primed larvae at the time of re-infection and, consequently, at the early time points after the infection with the lethal dose. Twenty four hours after the infection, these parameters were equally high in the non-primed and primed larvae. Accordingly, at the time of the injection of the lethal dose, certain immune-inducible genes were up-regulated. However, 24 h after the infection with the lethal dose, their expression in both groups was incomparably higher than at the time of the infection and, in most cases, it was as high in the primed larvae as in the non-primed ones. We found that only anti yeast-like activity was enhanced 24 h after the re-infection. This correlated with results obtained by testing the priming effect in heterologous systems: the primed animals did not exhibit higher resistance to the other pathogens tested. LA - English DB - MTMT ER - TY - JOUR AU - Wu, Ping AU - Shang, Qi AU - Huang, Haoling AU - Zhang, Shaolun AU - Zhong, Jinbo AU - Hou, Qirui AU - Guo, Xijie TI - Quantitative proteomics analysis provides insight into the biological role of Hsp90 in BmNPV infection in Bombyx mori JF - JOURNAL OF PROTEOMICS J2 - J PROTEOMICS VL - 203 PY - 2019 PG - 8 SN - 1874-3919 DO - 10.1016/j.jprot.2019.103379 UR - https://m2.mtmt.hu/api/publication/30781647 ID - 30781647 AB - Heat shock protein 90, an essential chaperone responsible for the correct maturation of key proteins, has been confirmed to facilitate Bombyx mori nucleopolyhedrovirus (BmNPV) proliferation but the mechanism is not clear. In this study, we use quantitative proteomics analysis to investigate the mechanism of Hsp90 in BmNPV replication. In total, 195 differentially expressed proteins (DEPs) were identified with 136 up-regulated proteins and 59 down-regulated proteins. The protein expression level of small heat shock proteins, immune-related proteins, cellular DNA repair-related proteins and zinc finger proteins is significantly enhanced while that of protein kinases is declined. KEGG pathway analysis reveals that DEPs are involved in longevity regulating pathway, mTOR signaling pathway, FoxO signaling pathway and Toll and Imd signaling pathway. Based on the DEPs results, we speculate that inhibition of Hsp90 suppresses the BmNPV infection may because it could not only stimulate the host innate immune, induce small heat shock proteins expression to maintain the cellular proteostasis but activate host transcription factors to bind to virus DNA or protein and subsequently hinder virus replication. The results will help understand the roles of Hsp90 in BmNPV infection and shed light on new clue to illustrate the molecular mechanism of silkworm-virus interaction.Significance: This is the first report on Hsp90 roles in BmNPV infection based on proteomic analysis. Our findings may provide new clue and research orientation to illustrate the molecular mechanism of silkworm-virus interaction and a set of BmHsp90 candidate clients, which may involve in BmNPV infection in BmN cells. LA - English DB - MTMT ER - TY - BOOK AU - Berman, J.J. TI - Precision medicine and the reinvention of human disease PB - Elsevier CY - New York, New York PY - 2018 SP - 1 EP - 398 SP - 398 SN - 9780128143940 DO - 10.1016/C2017-0-01874-8 UR - https://m2.mtmt.hu/api/publication/31872766 ID - 31872766 N1 - Cited By :2 Export Date: 15 February 2021 Correspondence Address: Berman, J.J. AB - Despite what you may have read in the popular press and in social media, Precision Medicine is not devoted to finding unique treatments for individuals, based on analyzing their DNA. To the contrary, the goal of Precision Medicine is to find general treatments that are highly effective for large numbers of individuals who fall into precisely diagnosed groups. We now know that every disease develops over time, through a sequence of defined biological steps, and that these steps may differ among individuals, based on genetic and environmental conditions. We are currently developing rational therapies and preventive measures, based on our precise understanding of the steps leading to the clinical expression of diseases. Precision Medicine and the Reinvention of Human Disease explains the scientific breakthroughs that have changed the way that we understand diseases, and reveals how medical scientists are using this new knowledge to launch a medical revolution. © 2018 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Bogus, Mieczyslawa I. AU - Ligeza-Zuber, Marta AU - Polanska, Marta A. AU - Mosiewicz, Maciej AU - Wloka, Emilia AU - Sobocinska, Martyna TI - Fungal infection causes changes in the number, morphology and spreading ability of Galleria mellonella haemocytes JF - PHYSIOLOGICAL ENTOMOLOGY J2 - PHYSIOL ENTOMOL VL - 43 PY - 2018 IS - 3 SP - 214 EP - 226 PG - 13 SN - 0307-6962 DO - 10.1111/phen.12246 UR - https://m2.mtmt.hu/api/publication/30512089 ID - 30512089 AB - The most effective and important strategy in the insect immune response is based on cellular reactions incorporating haemocytes. The present study uses Galleria mellonella (Lepidoptera: Pyralidae) as a host to study the pathogenesis caused by the entomopthoralean fungus Conidiobolus coronatus (Entomophthorales). Five types of haemocytes with different morphologies and behaviour are observed in the haemolymph of G. mellonella: granulocytes (GRs), plasmatocytes (PLs), spherulocytes (SPs), oenocytes (OEs) and prohaemocytes (PRs). During in vitro cultivation, three morphological subtypes of PLs are distinguished: flattened PLs, sun-like PLs and oval PLs. In fresh smears of haemolymph observed under phase-contrast microscopy, only flattened PLs are identified. No morphological changes are observed between fresh smears and in vitro cultures for GR, OE, SP and PR. Haemocytes cultured in vitro form a cellular network composed of PLs and GRs. Changes in the numbers, morphology and behaviour of haemocytes induced by fungal infection are compared with those observed in normally-developing untreated larvae. Infection results in a significant drop in the number of haemocyte types. Fresh smears of haemocytes from mycosed larvae reveal malformed OEs, vacuolized PLs and GRs, as well as PLs with apoptotic blebs. Haemocytes from mycosed larvae incubated in vitro look similar, with degranulated GRs and vacuolized PLs forming microaggregations, as well as deformed OEs; only the SPs remain unharmed. Fungal infection impairs the ability of haemocytes to attach and spread on the culture dish. The actin cytoskeleton of haemocytes from mycosed larvae appear disorganized. LA - English DB - MTMT ER - TY - JOUR AU - Danihlik, Jiri AU - Skrabisova, Maria AU - Lenobel, Rene AU - Sebela, Marek AU - Omar, Eslam AU - Petrivalsky, Marek AU - Crailsheim, Karl AU - Brodschneider, Robert TI - Does the Pollen Diet Influence the Production and Expression of Antimicrobial Peptides in Individual Honey Bees? JF - INSECTS J2 - INSECTS VL - 9 PY - 2018 IS - 3 PG - 12 SN - 2075-4450 DO - 10.3390/insects9030079 UR - https://m2.mtmt.hu/api/publication/30512086 ID - 30512086 AB - We investigated the importance of protein nutrition for honey bee immunity. Different protein diets (monofloral pollen of Helianthus spp., Sinapis spp., Asparagus spp., Castanea spp., a mixture of the four different pollen and the pollen substitute Feedbee(TM)) were fed to honey bees in cages ad libitum. After 18 days of feeding, apidaecin 1 isoforms concentration in the thorax were measured using nanoflow liquid chromatography coupled with mass spectrometry. Expression levels of genes, coding for apidaecins and abaecin in the abdomen were determined using quantitative PCR. The results indicate that protein-containing nutrition in adult worker honey bees can trigger certain metabolic responses. Bees without dietary protein showed lower apidaecin 1 isoforms concentrations. The significantly lowest concentration of apidaecin 1 isoforms was found in the group that was fed no pollen diet when compared to Asparagus, Castanea, Helianthus, and Sinapis pollen or the pollen supplement FeedBee(TM). Expression levels of the respective genes were also affected by the protein diets and different expression levels of these two antimicrobial peptides were found. Positive correlation between concentration and gene expression of apidaecins was found. The significance of feeding bees with different protein diets, as well as the importance of pollen nutrition for honey bee immunity is demonstrated. LA - English DB - MTMT ER - TY - JOUR AU - de Souza, Patricia Canteri AU - Caloni, Carla Custodio AU - Wilson, Duncan AU - Almeida, Ricardo Sergio TI - An Invertebrate Host to Study Fungal Infections, Mycotoxins and Antifungal Drugs: Tenebrio molitor JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 4 PY - 2018 IS - 4 PG - 12 SN - 2309-608X DO - 10.3390/jof4040125 UR - https://m2.mtmt.hu/api/publication/30512084 ID - 30512084 AB - Faced with ethical conflict and social pressure, researchers have increasingly chosen to use alternative models over vertebrates in their research. Since the innate immune system is evolutionarily conserved in insects, the use of these animals in research is gaining ground. This review discusses Tenebrio molitor as a potential model host for the study of pathogenic fungi. Larvae of T. molitor are known as cereal pests and, in addition, are widely used as animal and human feed. A number of studies on mechanisms of the humoral system, especially in the synthesis of antimicrobial peptides, which have similar characteristics to vertebrates, have been performed. These studies demonstrate the potential of T. molitor larvae as a model host that can be used to study fungal virulence, mycotoxin effects, host immune responses to fungal infection, and the action of antifungal compounds. LA - English DB - MTMT ER - TY - JOUR AU - Dickel, Franziska AU - Munch, Daniel AU - Amdam, Gro Vang AU - Mappes, Johanna AU - Freitak, Dalial TI - Increased survival of honeybees in the laboratory after simultaneous exposure to low doses of pesticides and bacteria JF - PLOS ONE J2 - PLOS ONE VL - 13 PY - 2018 IS - 1 PG - 18 SN - 1932-6203 DO - 10.1371/journal.pone.0191256 UR - https://m2.mtmt.hu/api/publication/27324962 ID - 27324962 LA - English DB - MTMT ER - TY - JOUR AU - Donati, I. AU - Cellini, A. AU - Buriani, G. AU - Mauri, S. AU - Spinelli, F. TI - Insect-mediated vectoring of Pseudomonas syringae pv. actinidiae JF - ACTA HORTICULTURAE: TECHNICAL COMMUNICATIONS OF ISHS J2 - ACTA HORTICULTURAE VL - 1218 PY - 2018 SP - 269 EP - 273 PG - 5 SN - 0567-7572 DO - 10.17660/ActaHortic.2018.1218.36 UR - https://m2.mtmt.hu/api/publication/31872767 ID - 31872767 N1 - Export Date: 15 February 2021 AB - From 2008, kiwifruit production and the total cultivated area decreased considerably, due to the pandemic spread of the bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa). This bacterium is able to infect host plants via natural openings or wounds, such as those caused by sucking insects. These insects are also known to be able to transmit other bacterial pathogens in different species. The present work aimed to investigate the role of Metcalfa pruinosa Say, 1830, one of the most common sucking insects to affect kiwifruit vines, in plant-to-plant transmission of Psa. The percentage of contaminated insects collected from infected orchards was evaluated to confirm the ecological relevance of M. pruinosa in the spread of bacterial canker of kiwifruit. This study demonstrated the survival of Psa in the M. pruinosa digestive trait, and the insect’s ability to vector the pathogen to healthy plants. © International Society for Horticultural Science. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Ebrahimi, Laleh AU - Shiri, Mohammadreza AU - Dunphy, Gary Brian TI - Effect of entomopathogenic nematode, Steinernema feltiae, on survival and plasma phenoloxidase activity of Helicoverpa armigera (Hb) (Lepidoptera: Noctuidae) in laboratory conditions JF - EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL J2 - EGYPT J BIOL PEST CO VL - 28 PY - 2018 PG - 4 SN - 1110-1768 DO - 10.1186/s41938-017-0016-x UR - https://m2.mtmt.hu/api/publication/27524973 ID - 27524973 LA - English DB - MTMT ER - TY - JOUR AU - Kennedy, David A. AU - Dwyer, Greg TI - Effects of multiple sources of genetic drift on pathogen variation within hosts JF - PLOS BIOLOGY J2 - PLOS BIOL VL - 16 PY - 2018 IS - 3 PG - 17 SN - 1544-9173 DO - 10.1371/journal.pbio.2004444 UR - https://m2.mtmt.hu/api/publication/30512081 ID - 30512081 AB - Changes in pathogen genetic variation within hosts alter the severity and spread of infectious diseases, with important implications for clinical disease and public health. Genetic drift may play a strong role in shaping pathogen variation, but analyses of drift in pathogens have oversimplified pathogen population dynamics, either by considering dynamics only at a single scale-such as within hosts or between hosts-or by making drastic simplifying assumptions, for example, that host immune systems can be ignored or that transmission bottlenecks are complete. Moreover, previous studies have used genetic data to infer the strength of genetic drift, whereas we test whether the genetic drift imposed by pathogen population processes can be used to explain genetic data. We first constructed and parameterized a mathematical model of gypsy moth baculovirus dynamics that allows genetic drift to act within and between hosts. We then quantified the genome-wide diversity of baculovirus populations within each of 143 field-collected gypsy moth larvae using Illumina sequencing. Finally, we determined whether the genetic drift imposed by host-pathogen population dynamics in our model explains the levels of pathogen diversity in our data. We found that when the model allows drift to act at multiple scales-including within hosts, between hosts, and between years-it can accurately reproduce the data, but when the effects of drift are simplified by neglecting transmission bottlenecks and stochastic variation in virus replication within hosts, the model fails. A de novo mutation model and a purifying selection model similarly fail to explain the data. Our results show that genetic drift can play a strong role in determining pathogen variation and that mathematical models that account for pathogen population growth at multiple scales of biological organization can be used to explain this variation. LA - English DB - MTMT ER - TY - JOUR AU - Klein, Laura D. AU - Huang, Jincui AU - Quinn, Elizabeth A. AU - Martin, Melanie A. AU - Breakey, Alicia A. AU - Gurven, Michael AU - Kaplan, Hillard AU - Valeggia, Claudia AU - Jasienska, Grazyna AU - Scelza, Brooke AU - Lebrilla, Carlito B. AU - Hinde, Katie TI - Variation among populations in the immune protein composition of mother's milk reflects subsistence pattern JF - EVOLUTION MEDICINE AND PUBLIC HEALTH J2 - EVOL MED PUBLIC HLTH PY - 2018 IS - 1 SP - 230 EP - 245 PG - 16 SN - 2050-6201 DO - 10.1093/emph/eoy031 UR - https://m2.mtmt.hu/api/publication/30512083 ID - 30512083 AB - Lay Summary: Adaptive immune proteins in mothers' milk are more variable than innate immune proteins across populations and subsistence strategies. These results suggest that the immune defenses in milk are shaped by a mother's environment throughout her life. LA - English DB - MTMT ER - TY - JOUR AU - Nascimento, M T C AU - Silva, K P AU - Garcia, M C F AU - Medeiros, M N AU - Machado, E A AU - Nascimento, S B AU - Saraiva, E M TI - DNA extracellular traps are part of the immune repertoire of Periplaneta americana JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 84 PY - 2018 SP - 62 EP - 70 PG - 9 SN - 0145-305X DO - 10.1016/j.dci.2018.01.012 UR - https://m2.mtmt.hu/api/publication/27524971 ID - 27524971 LA - English DB - MTMT ER - TY - JOUR AU - Ozturk, Gurhan AU - Cakici, Ozlem AU - Arikan, Huseyin TI - Morphological characterization of hemocyte types in some species belonging to Tettigoniidae and Pamphagidae (Insecta: Orthoptera) JF - TURKISH JOURNAL OF ZOOLOGY J2 - TURK J ZOOL VL - 42 PY - 2018 IS - 3 SP - 340 EP - 345 PG - 6 SN - 1300-0179 DO - 10.3906/zoo-1711-11 UR - https://m2.mtmt.hu/api/publication/27524974 ID - 27524974 LA - English DB - MTMT ER - TY - JOUR AU - Qu, Shuang AU - Wang, Sibao TI - Interaction of entomopathogenic fungi with the host immune system JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 83 PY - 2018 SP - 96 EP - 103 PG - 8 SN - 0145-305X DO - 10.1016/j.dci.2018.01.010 UR - https://m2.mtmt.hu/api/publication/27524972 ID - 27524972 LA - English DB - MTMT ER - TY - JOUR AU - Sheehan, Gerard AU - Garvey, Amy AU - Croke, Michael AU - Kavanagh, Kevin TI - Innate humoral immune defences in mammals and insects: The same, with differences ? JF - VIRULENCE J2 - VIRULENCE VL - 9 PY - 2018 IS - 1 SP - 1625 EP - 1639 PG - 15 SN - 2150-5594 DO - 10.1080/21505594.2018.1526531 UR - https://m2.mtmt.hu/api/publication/30512088 ID - 30512088 AB - The insect immune response demonstrates many similarities to the innate immune response of mammals and a wide range of insects is now employed to assess the virulence of pathogens and produce results comparable to those obtained using mammals. Many of the humoral responses in insects and mammals are similar (e.g. insect transglutaminases and human clotting factor XIIIa) however a number show distinct differences. For example in mammals, melanization plays a role in protection from solar radiation and in skin and hair pigmentation. In contrast, insect melanization acts as a defence mechanism in which the proPO system is activated upon pathogen invasion. Human and insect antimicrobial peptides share distinct structural and functional similarities, insects produce the majority of their AMPs from the fat body while mammals rely on production locally at the site of infection by epithelial/mucosal cells. Understanding the structure and function of the insect immune system and the similarities with the innate immune response of mammals will increase the attractiveness of using insects as in vivo models for studying host - pathogen interactions. LA - English DB - MTMT ER - TY - JOUR AU - Amemiya, K. AU - Bozue, J.A. AU - Cote, C.K. AU - Deshazer, D. AU - Soffler, C. AU - Welkos, S.L. AU - Worsham, P.L. TI - Animal Models for Melioidosis JF - Current Tropical Medicine Reports J2 - Curr Trop Med Rep VL - 4 PY - 2017 IS - 4 SP - 208 EP - 222 PG - 15 SN - 2196-3045 DO - 10.1007/s40475-017-0131-5 UR - https://m2.mtmt.hu/api/publication/31872763 ID - 31872763 N1 - Cited By :1 Export Date: 15 February 2021 Correspondence Address: Worsham, P.L.; Bacteriology Division, 1425 Porter St, Fort Detrick, United States; email: Patricia.l.worsham.civ@mail.mil AB - Purpose of Review: Development, testing, and evaluation of medical countermeasures for melioidosis are hampered by a lack of well-characterized and standardized animal models. Recent work has both refined existing animal models for this disease and identified new ones. Recent Findings: Head-to-head comparisons of mouse strains with varying susceptibility to the organism and using different routes of infection highlighted and confirmed important similarities and differences between murine models and exposure routes. Diabetic mouse models provided insight into the disease process in humans having this major risk factor. Large animal models, both livestock and non-human primate, have been established. Alternative (non-mammalian) models have been useful in identification of virulence factors and screening of therapeutic candidates. They hold potential for large-scale screening that would not be appropriate or practical for mammalian species. Summary: Recent advances in animal and alternative modeling will enhance our understanding of the organism and the disease process, as well as accelerating the development of medical countermeasures. © 2017, US Government (outside the USA). LA - English DB - MTMT ER - TY - JOUR AU - Arteaga, Blanco Luis Andres AU - Crispim, Josicelli Souza AU - Fernandes, Kenner Morais AU - de Oliveira, Leandro Licursi AU - Pereira, Monalessa Fabia AU - Soares, Bazzolli Denise Mara AU - Martins, Gustavo Ferreira TI - Differential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniae JF - CELL AND TISSUE RESEARCH J2 - CELL TISSUE RES VL - 370 PY - 2017 IS - 1 SP - 153 EP - 168 PG - 16 SN - 0302-766X DO - 10.1007/s00441-017-2653-5 UR - https://m2.mtmt.hu/api/publication/26891847 ID - 26891847 LA - English DB - MTMT ER - TY - JOUR AU - Cooper, D AU - Eleftherianos, I TI - Memory and specificity in the insect immune system: Current perspectives and future challenges JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 8 PY - 2017 SN - 1664-3224 DO - 10.3389/fimmu.2017.00539 UR - https://m2.mtmt.hu/api/publication/26674007 ID - 26674007 N1 - N1 Funding details: 1R01AI110675, NIAID, National Institute of Allergy and Infectious Diseases N1 Funding details: 1R21AI109517, NIAID, National Institute of Allergy and Infectious Diseases N1 Funding details: 1R56AI110675-01, NIAID, National Institute of Allergy and Infectious Diseases N1 Funding details: NIH, National Institutes of Health N1 Funding text: We thank members of the Department of Biological Sciences George Washington University (GWU) for critical reading of the manuscript. Research in the Eleftherianos laboratory is funded by grants from the National Institutes of Health-National Institute of Allergy and Infectious Diseases (1R01AI110675, 1R56AI110675-01, and 1R21AI109517) and the Columbian College of Arts and Sciences at George Washington University. LA - English DB - MTMT ER - TY - JOUR AU - Donati, Irene AU - Mauri, Sofia AU - Buriani, Giampaolo AU - Cellini, Antonio AU - Spinelli, Francesco TI - Role of Metcalfa pruinosa as a Vector for Pseudomonas syringae pv. actinidiae JF - PLANT PATHOLOGY JOURNAL (KOREA) J2 - PLANT PATHOLOGY J VL - 33 PY - 2017 IS - 6 SP - 554 EP - 560 PG - 7 SN - 1598-2254 DO - 10.5423/PPJ.OA.04.2017.0074 UR - https://m2.mtmt.hu/api/publication/27049969 ID - 27049969 LA - English DB - MTMT ER - TY - JOUR AU - Elhag, O AU - Zhou, D AU - Song, Q AU - Soomro, AA AU - Cai, M AU - Zheng, L AU - Yu, Z AU - Zhang, J TI - Screening, expression, purification and functional characterization of novel antimicrobial peptide genes from hermetia illucens (L.) JF - PLOS ONE J2 - PLOS ONE VL - 12 PY - 2017 IS - 1 SN - 1932-6203 DO - 10.1371/journal.pone.0169582 UR - https://m2.mtmt.hu/api/publication/26674006 ID - 26674006 LA - English DB - MTMT ER - TY - JOUR AU - Ishihara, Teruhito AU - Maruyama, Yuki AU - Furukawa, Seiichi TI - Gene expression and molecular characterization of a novel C-type lectin, encapsulation promoting lectin (EPL), in the rice armyworm, Mythimna separata JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 89 PY - 2017 SP - 51 EP - 57 PG - 7 SN - 0965-1748 DO - 10.1016/j.ibmb.2017.08.008 UR - https://m2.mtmt.hu/api/publication/27049413 ID - 27049413 N1 - College of Biological Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan School of BioSciences, The University of Melbourne, Parkville, VIC 3010, Australia Cited By :5 Export Date: 15 February 2021 CODEN: IBMBE Correspondence Address: Furukawa, S.; College of Biological Sciences, Tennodai 1-1-1, Japan; email: furukawa.seiichi.ew@u.tsukuba.ac.jp LA - English DB - MTMT ER - TY - JOUR AU - Rossoni, RD AU - Fuchs, BB AU - De Barros, PP AU - Dos Santos, Velloso M AU - Jorge, AOC AU - Junqueira, JC AU - Mylonakis, E TI - Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection JF - PLOS ONE J2 - PLOS ONE VL - 12 PY - 2017 IS - 3 SN - 1932-6203 DO - 10.1371/journal.pone.0173332 UR - https://m2.mtmt.hu/api/publication/26674008 ID - 26674008 LA - English DB - MTMT ER - TY - JOUR AU - Shattuck-Heidorn, H. AU - Reiches, M.W. AU - Prentice, A.M. AU - Moore, S.E. AU - Ellison, P.T. TI - Energetics and the immune system: Trade-offs associated with non-acute levels of CRP in adolescent Gambian girls JF - EVOLUTION MEDICINE AND PUBLIC HEALTH J2 - EVOL MED PUBLIC HLTH VL - 2017 PY - 2017 IS - 1 SP - 27 EP - 38 PG - 12 SN - 2050-6201 DO - 10.1093/emph/eow034 UR - https://m2.mtmt.hu/api/publication/31872773 ID - 31872773 N1 - Human Evolutionary Biology Department, Harvard University, 11 Divinity Ave, Cambridge, MA 02139, United States Department of Anthropology, University of Mass Boston, 100 Morrissey Blvd, Boston, MA 02125, United States MRC Unit, The Gambia and MRC International Nutrition Group, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, United Kingdom MRC Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, United Kingdom Cited By :10 Export Date: 15 February 2021 Correspondence Address: Shattuck-Heidorn, H.; Human Evolutionary Biology Department, 11 Divinity Ave, United States; email: faegre@fas.harvard.edu AB - Background and objectives: The human immune system is an ever-changing composition of innumerable cells and proteins, continually ready to respond to pathogens or insults. The cost of maintaining this state of immunological readiness is rarely considered. In this paper we aim to discern a cost to nonacute immune function by investigating how low levels of C-reactive protein (CRP) relate to other energetic demands and resources in adolescent Gambian girls. Methodology: Data from a longitudinal study of 66 adolescent girls was used to test hypotheses around investment in immune function. Non-acute (under 2 mg/L) CRP was used as an index of immune function. Predictor variables include linear height velocity, adiposity, leptin, and measures of energy balance. Results: Non-acute log CRP was positively associated with adiposity (β = 0.16, P<0.001, R2=0.17) and levels of the adipokine leptin (β = 1.17, P = 0.006, R2=0.09). CRP was also negatively associated with increased investment in growth, as measured by height velocity (β =-0.58, P<0.001, R2=0.13) and lean mass deposition β =-0.42, P = 0.005, R2=0.08). Relationships between adiposity and growth explained some, but not all, of this association. We do not find that CRP was related to energy balance. Conclusions and implications: These data support a hypothesis that investment in non-acute immune function is facultative, and sensitive to energetic resources and demands. We also find support for an adaptive association between the immune system and adipose tissue. © The Author(s) 2017. LA - English DB - MTMT ER - TY - JOUR AU - Shokal, U AU - Eleftherianos, I TI - Evolution and function of thioester-containing proteins and the complement system in the innate immune response JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 8 PY - 2017 SN - 1664-3224 DO - 10.3389/fimmu.2017.00759 UR - https://m2.mtmt.hu/api/publication/26674009 ID - 26674009 LA - English DB - MTMT ER - TY - JOUR AU - Skaldina, O AU - Sorvari, J TI - Wood ant colouration as an ecological indicator for the level of disturbance in managed coniferous forests JF - ECOLOGICAL INDICATORS J2 - ECOL INDIC VL - 72 PY - 2017 SP - 444 EP - 451 PG - 8 SN - 1470-160X DO - 10.1016/j.ecolind.2016.08.039 UR - https://m2.mtmt.hu/api/publication/26674005 ID - 26674005 N1 - N1 Funding details: CIMO, Centre for International Mobility N1 Funding text: We are grateful to Marja-Katariina Haatanen for kindly taking photos of the F. aquilonia gynes and Nataliia Buzhenina for assistance in artwork for figures. We are also grateful to the late Harri Hakkarainen for his help and company during the collection of field data. We thank Alexandre Roulin and an anonymous reviewer for their useful comments, which helped to improve the manuscript. Daniel Blande provided comments on the structure and language of the manuscript. The study was financially supported by the grants from the Centre for International Mobility (CIMO) and Kuopio Naturalists Society (KLYY). LA - English DB - MTMT ER - TY - JOUR AU - Wojda, Iwona TI - Temperature stress and insect immunity JF - JOURNAL OF THERMAL BIOLOGY: ENVIRONMENT EVOLUTION AND MEDICINE J2 - J THERM BIOL: ENVIRON EVOL MEDICINE VL - 68 PY - 2017 SP - 96 EP - 103 PG - 8 SN - 0306-4565 DO - 10.1016/j.jtherbio.2016.12.002 UR - https://m2.mtmt.hu/api/publication/26891848 ID - 26891848 LA - English DB - MTMT ER - TY - JOUR AU - Yang, Donghang AU - Zha, Guodong AU - Li, Xia AU - Gao, Han AU - Yu, Hanshou TI - Immune responses in the haemolymph and antimicrobial peptide expression in the abdomen of Apis mellifera challenged with Spiroplasma melliferum CH-1 JF - MICROBIAL PATHOGENESIS J2 - MICROB PATHOGENESIS VL - 112 PY - 2017 SP - 279 EP - 287 PG - 9 SN - 0882-4010 DO - 10.1016/j.micpath.2017.10.006 UR - https://m2.mtmt.hu/api/publication/27090699 ID - 27090699 LA - English DB - MTMT ER - TY - JOUR AU - Butt, T M AU - Coates, C J AU - Dubovskiy, I M AU - Ratcliffe, N A ED - Lovett, B ED - StLeger, RJ TI - Entomopathogenic Fungi: New Insights into Host-Pathogen Interactions JF - ADVANCES IN GENETICS J2 - ADV GENET VL - 94 PY - 2016 SP - 307 EP - 364 PG - 58 SN - 0065-2660 DO - 10.1016/bs.adgen.2016.01.006 UR - https://m2.mtmt.hu/api/publication/26203256 ID - 26203256 LA - English DB - MTMT ER - TY - JOUR AU - Champion, Olivia L AU - Wagley, Sariqa AU - Titball, Richard W TI - Galleria mellonella as a model host for microbiological and toxin research JF - VIRULENCE J2 - VIRULENCE VL - 7 PY - 2016 IS - 7 SP - 840 EP - 845 PG - 6 SN - 2150-5594 DO - 10.1080/21505594.2016.1203486 UR - https://m2.mtmt.hu/api/publication/26203255 ID - 26203255 N1 - Cited By :34 Export Date: 16 July 2020 Correspondence Address: Champion, O.L.; University of Exeter, Biosciences, Geoffrey Pope Building, Stocker Road, United Kingdom; email: O.L.Champion@exeter.ac.uk Chemicals/CAS: cyclosporin, 79217-60-0; destruxin, 11113-60-3; gelatinase, 9040-48-6; proprotein convertase 9; serine proteinase, 37259-58-8; Anti-Bacterial Agents; Toxins, Biological LA - English DB - MTMT ER - TY - JOUR AU - Dickel, Franziska AU - Freitak, Dalial AU - Mappes, Johanna TI - Long-Term Prophylactic Antibiotic Treatment: Effects on Survival, Immunocompetence and Reproduction Success of Parasemia plantaginis (Lepidoptera: Erebidae) JF - JOURNAL OF INSECT SCIENCE J2 - J INSECT SCI VL - 16 PY - 2016 PG - 7 SN - 1536-2442 DO - 10.1093/jisesa/iew035 UR - https://m2.mtmt.hu/api/publication/26015798 ID - 26015798 LA - English DB - MTMT ER - TY - JOUR AU - Faruck, Mohammad Omer AU - Yusof, Faridah AU - Chowdhury, Silvia TI - An overview of antifungal peptides derived from insect JF - PEPTIDES J2 - PEPTIDES VL - 80 PY - 2016 IS - Bagan SP - 80 EP - 88 PG - 9 SN - 0196-9781 DO - 10.1016/j.peptides.2015.06.001 UR - https://m2.mtmt.hu/api/publication/26003334 ID - 26003334 N1 - Funding Agency and Grant Number: Ministry of Higher Education, MalaysiaMinistry of Education, Malaysia [FRGS 013-023-0264]; Ministry of Higher Education, MalaysiaMinistry of Education, Malaysia [FRGS 013-023-0264] Funding text: We gratefully acknowledge the support of the Ministry of Higher Education, Malaysia, through the fundamental research grant scheme (Grant No. FRGS 013-023-0264). Export Date: 1 December 2020 CODEN: PPTDD Export Date: 3 December 2020 CODEN: PPTDD LA - English DB - MTMT ER - TY - JOUR AU - Gherlenda, Andrew N AU - Haigh, Anthony M AU - Moore, Ben D AU - Johnson, Scott N AU - Riegler, Markus TI - Climate change, nutrition and immunity: Effects of elevated CO2 and temperature on the immune function of an insect herbivore JF - JOURNAL OF INSECT PHYSIOLOGY J2 - J INSECT PHYSIOL VL - 85 PY - 2016 SP - 57 EP - 64 PG - 8 SN - 0022-1910 DO - 10.1016/j.jinsphys.2015.12.002 UR - https://m2.mtmt.hu/api/publication/25770903 ID - 25770903 LA - English DB - MTMT ER - TY - JOUR AU - Kazlauskas, Nadia AU - Klappenbach, Martin AU - Depino, Amaicha M AU - Locatelli, Fernando F TI - Sickness Behavior in Honey Bees JF - FRONTIERS IN PHYSIOLOGY J2 - FRONT PHYSIOL VL - 7 PY - 2016 PG - 10 SN - 1664-042X DO - 10.3389/fphys.2016.00261 UR - https://m2.mtmt.hu/api/publication/26015797 ID - 26015797 LA - English DB - MTMT ER - TY - JOUR AU - Li, Shaosong AU - Zhong, Xin AU - Kan, Xutian AU - Gu, Li AU - Sun, Hongxia AU - Zhang, Guren AU - Liu, Xin TI - De novo transcriptome analysis of Thitarodes jiachaensis before and after infection by the caterpillar fungus, Ophiocordyceps sinensis JF - GENE J2 - GENE VL - 580 PY - 2016 IS - 2 SP - 96 EP - 103 PG - 8 SN - 0378-1119 DO - 10.1016/j.gene.2016.01.007 UR - https://m2.mtmt.hu/api/publication/25770901 ID - 25770901 LA - English DB - MTMT ER - TY - JOUR AU - Naaz, Naiyar AU - Choudhary, Jaipal S AU - Prabhakar, Chandra S AU - Maurya, Moanaro Sudarshan TI - Identification and evaluation of cultivable gut bacteria associated with peach fruit fly, Bactrocera zonata (Diptera: Tephritidae) JF - PHYTOPARASITICA J2 - PHYTOPARASITICA VL - 44 PY - 2016 IS - 2 SP - 165 EP - 176 PG - 12 SN - 0334-2123 DO - 10.1007/s12600-016-0518-1 UR - https://m2.mtmt.hu/api/publication/26015799 ID - 26015799 N1 - Funding Agency and Grant Number: Ministry of Agriculture, Government of India through the National Initiative on Climate Resilient Agriculture (NICRA) project under the Indian Council of Agricultural Research (ICAR) [ICAR-RCER/RC R/E.F./2011/29] Funding text: This work was supported by the Ministry of Agriculture, Government of India through the National Initiative on Climate Resilient Agriculture (NICRA) project under the Indian Council of Agricultural Research (ICAR) (ICAR-RCER/RC R/E.F./2011/29). We are grateful to Dr. B.P. Bhatt (Director of institute) for giving valuable suggestions and providing laboratory facilities. LA - English DB - MTMT ER - TY - JOUR AU - Shu, Min AU - Mang, Dingze AU - Fu, Gege Sun AU - Tanaka, Shiho AU - Endo, Haruka AU - Kikuta, Shingo AU - Sato, Ryoichi TI - Mechanisms of nodule-specific melanization in the hemocoel of the silkworm, Bombyx mori JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 70 PY - 2016 SP - 10 EP - 23 PG - 14 SN - 0965-1748 DO - 10.1016/j.ibmb.2015.12.005 UR - https://m2.mtmt.hu/api/publication/25770902 ID - 25770902 LA - English DB - MTMT ER - TY - JOUR AU - Singh, D AU - Son, SY AU - Lee, CH TI - Perplexing metabolomes in fungal-insect trophic interactions: A Terra incognita of mycobiocontrol mechanisms JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 7 PY - 2016 SN - 1664-302X DO - 10.3389/fmicb.2016.01678 UR - https://m2.mtmt.hu/api/publication/26674010 ID - 26674010 N1 - N1 Funding details: MAFRA, Ministry of Agriculture, Food and Rural Affairs N1 Funding text: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. NRF-2014R1A2A1A11050884) and by the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea [as part of the (multi ministerial) Genome Technology to Business Translation Program] (Grant number 916005-2). LA - English DB - MTMT ER - TY - JOUR AU - Trowbridge, AM AU - Bowers, MD AU - Monson, RK TI - Conifer Monoterpene Chemistry during an Outbreak Enhances Consumption and Immune Response of an Eruptive Folivore JF - JOURNAL OF CHEMICAL ECOLOGY J2 - J CHEM ECOL VL - 42 PY - 2016 IS - 12 SP - 1281 EP - 1292 PG - 12 SN - 0098-0331 DO - 10.1007/s10886-016-0797-5 UR - https://m2.mtmt.hu/api/publication/26674011 ID - 26674011 N1 - N1 Funding details: 0500560, NSF, National Science Foundation N1 Funding details: University of Colorado Boulder LA - English DB - MTMT ER - TY - JOUR AU - Tsai, CJ-Y AU - Loh, JMS AU - Proft, T TI - Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing JF - VIRULENCE J2 - VIRULENCE VL - 7 PY - 2016 IS - 3 SP - 214 EP - 229 PG - 16 SN - 2150-5594 DO - 10.1080/21505594.2015.1135289 UR - https://m2.mtmt.hu/api/publication/25801794 ID - 25801794 LA - English DB - MTMT ER - TY - CHAP AU - Zaila, K.E. AU - Cho, D. AU - Chang, W.-J. ED - Witzany, Guenther Nowacki Mariusz ED - Witzany, Guenther ED - Nowacki, Mariusz TI - Interactions between parasitic ciliates and their hosts: Ichthyophthirius multifiliis and cryptocaryon irritans as examples T2 - Biocommunication of Ciliates PB - Springer Netherlands SN - 9783319322117 PY - 2016 SP - 327 EP - 350 PG - 24 DO - 10.1007/978-3-319-32211-7_18 UR - https://m2.mtmt.hu/api/publication/31872774 ID - 31872774 N1 - Department of Biology, Hamilton College, Clinton, NY 13323, United States Institute of Molecular Biology, Academia Sinica, Taipei, 11529, Taiwan Cited By :2 Export Date: 15 February 2021 Correspondence Address: Chang, W.-J.; Department of Biology, United States; email: wchang@hamilton.edu AB - Among all known parasitic ciliates, Ichthyophthirius multifiliis and Cryptocaryon irritans are probably the two best studied "model parasites," which cause the white spot diseases in freshwater and marine fish, respectively. These two parasites are not phylogenetically closely related, but share very similar life cycle and pathological manifestations. Both parasites invade and feed in the epithelial layer of the skin and gill, and trigger both innate and adaptive immune responses in fish hosts, locally and systemically. The parasite-host interactions not only help add to our knowledge of fish immunity, but also shed light on research regarding other less well-studied ciliate parasites. Future research directions are also discussed in this chapter. © Springer International Publishing Switzerland 2016. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - de Oliveira, Haroldo C AU - da Silva, Julhiany de Fatima AU - Scorzoni, Liliana AU - Marcos, Caroline M AU - Rossi, Sueen A AU - de Paula, e Silva Ana C A AU - Assato, Patricia A AU - da Silva, Rosangela A M AU - Fusco-Almeida, Ana M AU - Mendes-Giannini, Maria J S TI - Importance of adhesins in virulence of Paracoccidioides spp. JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 6 PY - 2015 PG - 14 SN - 1664-302X DO - 10.3389/fmicb.7015.00303 UR - https://m2.mtmt.hu/api/publication/25283690 ID - 25283690 LA - English DB - MTMT ER - TY - JOUR AU - de Oliveira, HC AU - da Silva, JF AU - Scorzoni, L AU - Marcos, CM AU - Rossi, SA AU - de Paula, e Silva ACA AU - Assato, PA AU - da Silva, RAM AU - Fusco-Almeida, AM AU - Mendes-Giannini, MJS TI - Importance of adhesins in virulence of Paracoccidioides spp. JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 6 PY - 2015 SN - 1664-302X DO - 10.3389/fmicb.2015.00303 UR - https://m2.mtmt.hu/api/publication/24983669 ID - 24983669 LA - English DB - MTMT ER - TY - JOUR AU - Liang, JB AU - Wang, T AU - Xiang, ZH AU - He, NJ TI - Tweedle cuticular protein BmCPT1 is involved in innate immunity by participating in recognition of Escherichia coli JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 58 PY - 2015 SP - 76 EP - 88 PG - 13 SN - 0965-1748 DO - 10.1016/j.ibmb.2014.11.004 UR - https://m2.mtmt.hu/api/publication/24728915 ID - 24728915 LA - English DB - MTMT ER - TY - JOUR AU - Meng, Qian AU - Yu, Hai-Ying AU - Zhang, Huan AU - Zhu, Wei AU - Wang, Meng-Long AU - Zhang, Ji-Hong AU - Zhou, Gui-Ling AU - Li, Xuan AU - Qin, Qi-Lian AU - Hu, Song-Nian AU - Zou, Zhen TI - Transcriptomic insight into the immune defenses in the ghost moth, Hepialus xiaojinensis, during an Ophiocordyceps sinensis fungal infection JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 64 PY - 2015 SP - 1 EP - 15 PG - 15 SN - 0965-1748 DO - 10.1016/j.ibmb.2015.06.014 UR - https://m2.mtmt.hu/api/publication/25283689 ID - 25283689 LA - English DB - MTMT ER - TY - JOUR AU - Reavey, CE AU - Silva, FWS AU - Cotter, SC TI - Bacterial infection increases reproductive investment in burying beetles JF - INSECTS J2 - INSECTS VL - 6 PY - 2015 IS - 4 SP - 926 EP - 942 PG - 17 SN - 2075-4450 DO - 10.3390/insects6040926 UR - https://m2.mtmt.hu/api/publication/25801796 ID - 25801796 LA - English DB - MTMT ER - TY - JOUR AU - Wu, G AU - Li, M AU - Liu, Y AU - Ding, Y AU - Yi, Y TI - The specificity of immune priming in silkworm, Bombyx mori, is mediated by the phagocytic ability of granular cells JF - JOURNAL OF INSECT PHYSIOLOGY J2 - J INSECT PHYSIOL VL - 81 PY - 2015 SP - 60 EP - 68 PG - 9 SN - 0022-1910 DO - 10.1016/j.jinsphys.2015.07.004 UR - https://m2.mtmt.hu/api/publication/24983667 ID - 24983667 LA - English DB - MTMT ER - TY - JOUR AU - Yue, Y-J AU - Tang, X-D AU - Xu, L AU - Yan, W AU - Li, Q-L AU - Xiao, S-Y AU - Fu, X-L AU - Wang, W AU - Li, N AU - Shen, Z-Y TI - Early responses of silkworm midgut to microsporidium infection - A Digital Gene Expression analysis JF - JOURNAL OF INVERTEBRATE PATHOLOGY J2 - J INVERTEBR PATHOL VL - 124 PY - 2015 SP - 6 EP - 14 PG - 9 SN - 0022-2011 DO - 10.1016/j.jip.2014.10.003 UR - https://m2.mtmt.hu/api/publication/24983671 ID - 24983671 LA - English DB - MTMT ER - TY - JOUR AU - Azeez, OI AU - Meintjes, R AU - Chamunorwa, JP TI - Fat body, fat pad and adipose tissues in invertebrates and vertebrates: the nexus JF - LIPIDS IN HEALTH AND DISEASE J2 - LIPIDS HEALTH DIS VL - 13 PY - 2014 SN - 1476-511X DO - 10.1186/1476-511X-13-71 UR - https://m2.mtmt.hu/api/publication/24094417 ID - 24094417 LA - English DB - MTMT ER - TY - BOOK AU - Berman, JJ TI - Rare Diseases and Orphan Drugs: Keys to Understanding and Treating the Common Diseases PY - 2014 SN - 9780124199880 DO - 10.1016/C2013-0-09838-6 UR - https://m2.mtmt.hu/api/publication/27617323 ID - 27617323 N1 - Cited By :21 Export Date: 18 March 2024 Correspondence Address: Berman, J.J. LA - English DB - MTMT ER - TY - JOUR AU - Buckley, AA AU - Faustoferri, RC AU - Quivey, Jr RG TI - β-Phosphoglucomutase contributes to aciduricity in streptococcus mutans JF - MICROBIOLOGY-SGM J2 - MICROBIOL-SGM VL - 160 PY - 2014 IS - PART 4 SP - 818 EP - 827 PG - 10 SN - 1350-0872 DO - 10.1099/mic.0.075754-0 UR - https://m2.mtmt.hu/api/publication/24983673 ID - 24983673 LA - English DB - MTMT ER - TY - JOUR AU - Ebrahimi, L AU - Niknam, G AU - Dunphy, GB AU - Toorchi, M TI - Effect of an entomopathogenic nematode, Steinernema carpocapsae on haemocyte profile and phenoloxidase activity of the Colorado potato beetle, Leptinotarsa decemlineata JF - BIOCONTROL SCIENCE AND TECHNOLOGY J2 - BIOCONTROL SCI TECHN VL - 24 PY - 2014 IS - 12 SP - 1383 EP - 1393 PG - 11 SN - 0958-3157 DO - 10.1080/09583157.2014.942595 UR - https://m2.mtmt.hu/api/publication/24313037 ID - 24313037 LA - English DB - MTMT ER - TY - JOUR AU - Kennedy, DA AU - Dukic, V AU - Dwyer, G TI - Pathogen Growth in Insect Hosts: Inferring the Importance of Different Mechanisms Using Stochastic Models and Response-Time Data JF - AMERICAN NATURALIST J2 - AM NAT VL - 184 PY - 2014 IS - 3 SP - 407 EP - 423 PG - 17 SN - 0003-0147 DO - 10.1086/677308 UR - https://m2.mtmt.hu/api/publication/24313035 ID - 24313035 LA - English DB - MTMT ER - TY - JOUR AU - Lyupina, YV AU - Orlova, AS AU - Gornostayev, NG AU - Karpova, YD AU - Mikhailov, VS AU - Sharova, NP TI - Plasticity of nervous and immune systems in different species: The role of proteasomes JF - ZHURNAL OBSHCHEI BIOLOGII J2 - ZH OBSHCH BIOL VL - 75 PY - 2014 IS - 1 SP - 3 EP - 24 PG - 22 SN - 0044-4596 UR - https://m2.mtmt.hu/api/publication/25122863 ID - 25122863 LA - Russian DB - MTMT ER - TY - JOUR AU - Marringa, WJ AU - Krueger, MJ AU - Burritt, NL AU - Burritt, JB TI - Honey bee hemocyte profiling by flow cytometr JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 10 SN - 1932-6203 DO - 10.1371/journal.pone.0108486 UR - https://m2.mtmt.hu/api/publication/24983677 ID - 24983677 LA - English DB - MTMT ER - TY - JOUR AU - Ode, PJ AU - Johnson, SN AU - Moore, BD TI - Atmospheric change and induced plant secondary metabolites - Are we reshaping the building blocks of multi-trophic interactions? JF - CURRENT OPINION IN INSECT SCIENCE J2 - CURR OPIN INSECT SCI VL - 5 PY - 2014 IS - 1 SP - 57 EP - 65 PG - 9 SN - 2214-5745 DO - 10.1016/j.cois.2014.09.006 UR - https://m2.mtmt.hu/api/publication/24983679 ID - 24983679 N1 - N1 Funding Details: UWS, University of Western Sydney LA - English DB - MTMT ER - TY - JOUR AU - Polkki, M AU - Kangassalo, K AU - Rantala, MJ TI - Effects of Interaction between Temperature Conditions and Copper Exposure on Immune Defense and Other Life-History Traits of the Blow Fly Protophormia terraenovae JF - ENVIRONMENTAL SCIENCE & TECHNOLOGY J2 - ENVIRON SCI TECHNOL VL - 48 PY - 2014 IS - 15 SP - 8793 EP - 8799 PG - 7 SN - 0013-936X DO - 10.1021/es501880b UR - https://m2.mtmt.hu/api/publication/24313036 ID - 24313036 LA - English DB - MTMT ER - TY - JOUR AU - Smith, Alexis A AU - Pal, Utpal TI - Immunity-related genes in Ixodes scapularis-perspectives from genorne information JF - FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY J2 - FRONT CELL INFECT MI VL - 4 PY - 2014 PG - 12 SN - 2235-2988 DO - 10.3389/fcimb.2014.00116 UR - https://m2.mtmt.hu/api/publication/24863914 ID - 24863914 N1 - Cited By :47 Export Date: 9 August 2022 Correspondence Address: Pal, U.; Department of Veterinary Medicine, University of Maryland, Building 795, 8075 Greenmead Drive, College Park, MD 20742, United States; email: upal@umd.edu Molecular Sequence Numbers: GENBANK: AAV63544; Chemicals/CAS: proteinase, 9001-92-7; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antimicrobial Cationic Peptides; Reactive Oxygen Species Funding details: National Institute of Allergy and Infectious Diseases, NIAID, R56AI080615 LA - English DB - MTMT ER - TY - JOUR AU - Tokura, A AU - Fu, GS AU - Sakamoto, M AU - Endo, H AU - Tanaka, S AU - Kikuta, S AU - Tabunoki, H AU - Sato, R TI - Factors functioning in nodule melanization of insects and their mechanisms of accumulation in nodules JF - JOURNAL OF INSECT PHYSIOLOGY J2 - J INSECT PHYSIOL VL - 60 PY - 2014 SP - 40 EP - 49 PG - 10 SN - 0022-1910 DO - 10.1016/j.jinsphys.2013.11.003 UR - https://m2.mtmt.hu/api/publication/24094418 ID - 24094418 LA - English DB - MTMT ER - TY - JOUR AU - Ahmed, AM TI - Bacillus thuringiensis Induces Cellular Stress in the Mosquito Vector, Culex pipiens, Prior to Death JF - PAKISTAN JOURNAL OF ZOOLOGY J2 - PAK J ZOOL VL - 45 PY - 2013 IS - 1 SP - 129 EP - 139 PG - 11 SN - 0030-9923 UR - https://m2.mtmt.hu/api/publication/23355737 ID - 23355737 LA - English DB - MTMT ER - TY - JOUR AU - Arai, I AU - Ohta, M AU - Suzuki, A AU - Tanaka, S AU - Yoshizawa, Y AU - Sato, R TI - Immunohistochemical analysis of the role of hemocytin in nodule formation in the larvae of the silkworm, Bombyx mori JF - JOURNAL OF INSECT SCIENCE J2 - J INSECT SCI VL - 13 PY - 2013 SN - 1536-2442 UR - https://m2.mtmt.hu/api/publication/24094420 ID - 24094420 LA - English DB - MTMT ER - TY - JOUR AU - Browne, N AU - Heelan, M AU - Kavanagh, K TI - An analysis of the structural and functional similarities of insect hemocytes and mammalian phagocytes JF - VIRULENCE J2 - VIRULENCE VL - 4 PY - 2013 IS - 7 SP - 597 EP - 603 PG - 7 SN - 2150-5594 DO - 10.4161/viru.25906 UR - https://m2.mtmt.hu/api/publication/23469566 ID - 23469566 LA - English DB - MTMT ER - TY - JOUR AU - Fujiyuki, T. AU - Takeda, T. AU - Hamamoto, H. AU - Ishii, K. AU - Urai, M. AU - Kataoka, K. AU - Shibata, S. AU - Sekimizu, K. TI - Evaluation of Innate Immune Stimulating Activity of Polysaccharides Using a Silkworm (Bombyx Mori) Muscle Contraction Assay JF - DRUG DISCOVERIES & THERAPEUTICS J2 - DRUG DISCOV THER VL - 6 PY - 2013 IS - 2 SP - 88 EP - 93 PG - 6 SN - 1881-7831 DO - 10.5582/ddt.2012.v6.2.88 UR - https://m2.mtmt.hu/api/publication/31872768 ID - 31872768 N1 - Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan Genome Pharmaceuticals Institute Co., Ltd., Tokyo, Japan Department of Sports Health, Nagoya Gakuin University, Seto, Aichi, Japan Cited By :7 Export Date: 15 February 2021 Correspondence Address: Sekimizu, K.; Graduate School of Pharmaceutical Sciences, , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan AB - In silkworm larvae, the mature form of paralytic peptide (PP), an insect cytokine, is produced from pro-PP in association with activation of innate immune responses, resulting in slow muscle contraction. We utilized this reaction, muscle contraction in silkworms coupled with innate immunity stimulation, to quantitatively measure the innate immune stimulating activity of various natural polysaccharides. β-Glucan of Gyrophora esculenta (GE-3), fucoidan from sporophyll of Undaria pinnatifida, and curldan induced silkworm muscle contraction. We further demonstrated that GE-3 had therapeutic effects on silkworms infected by baculovirus. Based on these findings, we propose that the silkworm muscle contraction assay is useful for screening substances that stimulate innate immunity before evaluating therapeutic effectiveness in mammals. © 2012, International Research and Cooperation Association for Bio & Socio-Sciences Advancement. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Gunaratna, RT AU - Jiang, HB TI - A comprehensive analysis of the Manduca sexta immunotranscriptome JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 39 PY - 2013 IS - 4 SP - 388 EP - 398 PG - 11 SN - 0145-305X DO - 10.1016/j.dci.2012.10.004 UR - https://m2.mtmt.hu/api/publication/23355891 ID - 23355891 LA - English DB - MTMT ER - TY - JOUR AU - Kim, PS AU - Shin, NR AU - Kim, JY AU - Yun, JH AU - Hyun, DW AU - Bae, JW TI - Gibbsiella papilionis sp nova, isolated from the intestinal tract of the butterfly Mycalesis gotama, and emended description of the genus Gibbsiella JF - INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY J2 - INT J SYST EVOL MICR VL - 63 PY - 2013 SP - 2607 EP - 2611 PG - 5 SN - 1466-5026 DO - 10.1099/ijs.0.045880-0 UR - https://m2.mtmt.hu/api/publication/23355736 ID - 23355736 N1 - PN 7 LA - English DB - MTMT ER - TY - BOOK AU - Klowden, MJ TI - Physiological Systems in Insects: Third Edition PB - Elsevier Inc. CY - Amsterdam PY - 2013 SN - 9780124158191 DO - 10.1016/C2011-0-04120-0 UR - https://m2.mtmt.hu/api/publication/27009925 ID - 27009925 LA - English DB - MTMT ER - TY - JOUR AU - Martin, RR AU - Naftel, SJ AU - Doucet, SM AU - Hanley, D AU - Weisner, CG TI - Synchrotron radiation analysis in the study of pollution in the ring-billed gull (larus delawarensis): a novel application of the technique JF - X-RAY SPECTROMETRY J2 - X-RAY SPECTROM VL - 42 PY - 2013 IS - 6 SP - 437 EP - 441 PG - 5 SN - 0049-8246 DO - 10.1002/xrs.2500 UR - https://m2.mtmt.hu/api/publication/23466769 ID - 23466769 LA - English DB - MTMT ER - TY - JOUR AU - Mesa-Arango, AC AU - Forastiero, A AU - Bernal-Martinez, L AU - Cuenca-Estrella, M AU - Mellado, E AU - Zaragoza, O TI - The non-mammalian host Galleria mellonella can be used to study the virulence of the fungal pathogen Candida tropicalis and the efficacy of antifungal drugs during infection by this pathogenic yeast JF - MEDICAL MYCOLOGY J2 - MED MYCOL VL - 51 PY - 2013 IS - 5 SP - 461 EP - 472 PG - 12 SN - 1369-3786 DO - 10.3109/13693786.2012.737031 UR - https://m2.mtmt.hu/api/publication/23355732 ID - 23355732 LA - English DB - MTMT ER - TY - JOUR AU - Oh, SJ AU - Shin, N-R AU - Hyun, D-W AU - Kim, PS AU - Kim, JY AU - Kim, M-S AU - Yun, J-H AU - Bae, J-W TI - Weissella diestrammenae sp. nov., isolated from the gut of a camel cricket (Diestrammena coreana) JF - INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY J2 - INT J SYST EVOL MICR VL - 63 PY - 2013 IS - PART8 SP - 2951 EP - 2956 PG - 6 SN - 1466-5026 DO - 10.1099/ijs.0.047548-0 UR - https://m2.mtmt.hu/api/publication/23469565 ID - 23469565 LA - English DB - MTMT ER -