@article{MTMT:34597451, title = {New insights into changing honey bee (Apis mellifera) immunity molecules pattern and fatty acid esters, in responses to Ascosphaera apis infection}, url = {https://m2.mtmt.hu/api/publication/34597451}, author = {Elhoseny, Manar M. M. and El-Didamony, Samia E. and Atwa, Wedad A. A. and Althoqapy, Azza A. and Gouda, Hend I. A.}, doi = {10.1016/j.jip.2023.108028}, journal-iso = {J INVERTEBR PATHOL}, journal = {JOURNAL OF INVERTEBRATE PATHOLOGY}, volume = {202}, unique-id = {34597451}, issn = {0022-2011}, abstract = {Monitoring of metabolite changes could provide valuable insights into disturbances caused by an infection and furthermore, could be used to define the status of an organism as healthy or diseased and define what could be defensive elements against the infection. The present investigation conducted a gas chromatography-mass spectrometry (GC/MS) for haemolymph of larval honey bees (Apis mellifera L.) infected with the fungal pathogen Ascosphaera apis in comparison with control haemolymph non-infected insects. Results revealed that the path-ogen caused a general disturbance of metabolites detected in the haemolymph of the honey bee. The majority of metabolites identified before and after infection were fatty acid esters. The disease caused an elevation in levels of methyl oleate, methyl palmitate, and methyl stearate, respectively. Further, the disease drove to the disap-pearance of methyl palmitoleate, and methyl laurate. Conversely, methyl linolelaidate, and ethyl oleate were identified only in infected larvae. A high reduction in diisooctyl phthalate was recorded after the infection. Interestingly, antimicrobial activities were confirmed for haemolymph of infected honey bee larvae. In spite of the presence of some previously known bioactive compounds in healthy larvae there were no antimicrobial activities.}, keywords = {GC/MS; Honey bee; Ascosphaera apis; Immunity pattern; FAEs}, year = {2024}, eissn = {1096-0805} } @article{MTMT:33873118, title = {Honeybee wings hold antibiofouling and antimicrobial clues for improved applications in health care and industries}, url = {https://m2.mtmt.hu/api/publication/33873118}, author = {Ewunkem, Akamu J. and Beard, A'lyiha F. and Justice, Brittany L. and Peoples, Sabrina L. and Meixner, Jeffery A. and Kemper, Watson and Iloghalu, Uchenna B.}, doi = {10.3934/microbiol.2023018}, journal-iso = {AIMS MICROBIOLOGY}, journal = {AIMS MICROBIOLOGY}, volume = {9}, unique-id = {33873118}, issn = {2471-1888}, abstract = {Natural surfaces with remarkable properties and functionality have become the focus of intense research. Heretofore, the natural antimicrobial properties of insect wings have inspired research into their applications. The wings of cicadas, butterflies, dragonflies, and damselflies have evolved phenomenal anti-biofouling and antimicrobial properties. These wings are covered by periodic topography ranging from highly ordered hexagonal arrays of nanopillars to intricate "Christmas-tree" like structures with the ability to kill microbes by physically rupturing the cell membrane. In contrast, the topography of honeybee wings has received less attention. The role topography plays in antibiofouling, and antimicrobial activity of honeybee wings has never been investigated. Here, through antimicrobial and electron microscopy studies, we showed that pristine honeybee wings displayed no microbes on the wing surface. Also, the wings displayed antimicrobial properties that disrupt microbial cells and inhibit their growth. The antimicrobial activities of the wings were extremely effective at inhibiting the growth of Gram-negative bacterial cells when compared to Gram-positive bacterial cells. The fore wing was effective at inhibiting the growth of Gram-negative bacteria compared to Gram-positive samples. Electron microscopy revealed that the wings were studded with an array of rough, sharp, and pointed pillars that were distributed on both the dorsal and ventral sides, which enhanced anti-biofouling and antimicrobial effects. Our findings demonstrate the potential benefits of incorporating honeybee wings nanopatterns into the design of antibacterial nanomaterials which can be translated into countless applications in healthcare and industry.}, keywords = {BACTERIA; WORKERS; Antimicrobial; WINGS; honeybee; Anti-biofouling}, year = {2023}, pages = {332-345} } @article{MTMT:34261033, title = {Vertebrate and Invertebrate Animal and New In Vitro Models for Studying Neisseria Biology}, url = {https://m2.mtmt.hu/api/publication/34261033}, author = {Girgis, Michael M. and Christodoulides, Myron}, doi = {10.3390/pathogens12060782}, journal-iso = {PATHOGENS}, journal = {PATHOGENS}, volume = {12}, unique-id = {34261033}, abstract = {The history of Neisseria research has involved the use of a wide variety of vertebrate and invertebrate animal models, from insects to humans. In this review, we itemise these models and describe how they have made significant contributions to understanding the pathophysiology of Neisseria infections and to the development and testing of vaccines and antimicrobials. We also look ahead, briefly, to their potential replacement by complex in vitro cellular models.}, keywords = {INFECTION; immunology; VACCINE; animal model; invertebrate; vertebrate; Neisseria meningitidis; neisseria gonorrhoeae}, year = {2023}, eissn = {2076-0817}, orcid-numbers = {Christodoulides, Myron/0000-0002-9663-4731} } @article{MTMT:34261031, title = {Immunosuppressive effect of Plantago major on the innate immunity of Galleria mellonella}, url = {https://m2.mtmt.hu/api/publication/34261031}, author = {Kaya, Serhat}, doi = {10.7717/peerj.15982}, journal-iso = {PEERJ}, journal = {PEERJ}, volume = {11}, unique-id = {34261031}, issn = {2167-8359}, abstract = {Greater plantain (Plantago major), a medicinal plant species, is used in folk medicine for the treatment of various diseases in many countries of the world. Different studies have shown that the bioactive components contained in the plant have a dual effect. It was also reported that in vivo and in vitro studies showed different results. The aim of the study was to determine the effects of P. major extract on the hemocyte-mediated and humoral immune responses of the invertebrate model organism Galleria mellonella, which is widely used in immune studies. In the evaluation of these effects, total hemocyte count, encapsulation, melanization, phenoloxidase, superoxide dismutase, catalase, malondialdehyde and total protein parameters were evaluated. The results of the study showed that the total hemocyte count did not change, that the encapsulation responses decreased, that the melanization responses and phenoloxidase activity increased and that the superoxide dismutase activity decreased. As a result, it was determined that high doses of P. major had negative effects on cell-mediated immunity and antioxidant defence and positive effects on melanization. High doses and continuous use of P. major may have negative effects on living things.}, keywords = {Galleria mellonella; humoral immune responses; Plantago major; Total hemocyte count; Cell-mediated immune responses}, year = {2023}, eissn = {2167-8359} } @article{MTMT:34261032, title = {Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection}, url = {https://m2.mtmt.hu/api/publication/34261032}, author = {Li, Miaomiao and Zhang, Jihong and Qin, Qilian and Zhang, Huan and Li, Xuan and Wang, Hongtuo and Meng, Qian}, doi = {10.3390/microorganisms11092361}, journal-iso = {MICROORGANISMS}, journal = {MICROORGANISMS}, volume = {11}, unique-id = {34261032}, issn = {2076-2607}, abstract = {Ophiocordyceps sinensis exhibits more than 5 months of vegetative growth in Thitarodes xiaojinensis hemocoel. The peculiar development process of O. sinensis has been elucidated through morphological observation and omics technology; however, little information has been reported regarding the changes that occur in the host T. xiaojinensis. The RNA sequencing data showed that when O. sinensis blastospores were in the proliferative stage, the greatest change in the infected larval fat body was the selectively upregulated immune recognition and antimicrobial peptide genes. When O. sinensis blastospores were in the stationary stage, the immune pathways of T. xiaojinensis reverted to normal levels, which coincides with the successful settlement of O. sinensis. Pathway enrichment analysis showed a higher expression of genes involved in energy metabolism pathway in this stage. Metabolomic analyses revealed a reduction of amino acids and lipids in hemolymph, but an upregulation of lipids in the fat body of the host larvae after O. sinensis infection. We present the first transcriptome integrated with the metabolome study of T. xiaojinensis infected by O. sinensis. It will improve our understanding of the interaction mechanisms between the host and entomopathogenic fungi, and facilitate future functional studies of genes and pathways involved in these interactions.}, keywords = {lipid metabolism; amino acid; IMMUNE DEFENSE; Integrated omics analysis; fungus-host interaction}, year = {2023}, eissn = {2076-2607} } @article{MTMT:33181293, title = {The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs}, url = {https://m2.mtmt.hu/api/publication/33181293}, author = {Alejandro, Alvarado-Delgado and Lilia, Juarez-Palma and Jesus, Maritinez-Bartneche and Mario Henry, Rodriguez}, doi = {10.1186/s13071-022-05363-y}, journal-iso = {PARASITE VECT}, journal = {PARASITES AND VECTORS}, volume = {15}, unique-id = {33181293}, issn = {1756-3305}, abstract = {Background: Antimicrobial peptides (AMPs) participate in the humoral immune response of insects eliminating invasive microorganisms. The immune deficiency pathway (IMD) and Toll are the main pathways by which the synthesis of these molecules is regulated in response to Gram-negative (IMD pathway) or Gram-positive (Toll pathway) bacteria. Various pattern-recognition receptors (PRRs) participate in the recognition of microorganisms, such as pgrp-Ic and toll, which trigger signaling cascades and activate NF-kappa B family transcription factors, such as relish, that translocate to the cell nucleus, mainly in the fat body, inducing AMP gene transcription.Methods: T. pallidipennis inhibited in Tppgrp-lc,Tptoll, and Tprelish were challenged with E. coli and M. luteus to analyze the expression of AMPs transcripts in the fat body and to execute survival assays.Results: In this work we investigated the participation of the pgrp-lc and toll receptor genes and the relish transcription factor (designated as Tppgrp-lc, Tptoll, and Tprelish), in the transcriptional regulation of defensin B, prolixicin, and lysozyme B in Triatoma pallidipennis, one of the main vectors of Chagas disease. AMP transcript abundance was higher in the fat body of blood-fed than non-fed bugs. Challenge with Escherichia coli or Micrococcus luteus induced differential increases in AMP transcripts. Additionally, silencing of Tppgrp-lc, Tptoll, and Tprelish resulted in reduced AMP transcription and survival of bugs after a bacterial challenge.Conclusions: Our findings demonstrated that the IMD and Toll pathways in T. pallidipennis preferentially respond to Gram-negative and Gram-positive bacteria, respectively, by increasing the expression of AMP transcripts, but crossinduction also occurs.}, keywords = {IMD pathway; T. pallidipennis; Tppgrp-lc; Tptoll; Tprelish}, year = {2022}, eissn = {1756-3305} } @article{MTMT:32654955, title = {Transcriptomic analysis provides insights into the immune responses and nutrition in Ostrinia furnacalis larvae parasitized by Macrocentrus cingulum}, url = {https://m2.mtmt.hu/api/publication/32654955}, author = {Chen, K. and Song, J. and Song, Q. and Dou, X. and Wang, Y. and Wei, Y. and Chen, J. and Wang, L. and Alradi, M.F. and Liu, X. and Han, Z. and Feng, C.}, doi = {10.1002/arch.21863}, journal-iso = {ARCH INSECT BIOCHEM}, journal = {ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY}, volume = {109}, unique-id = {32654955}, issn = {0739-4462}, abstract = {Macrocentrus cingulum is a principal endoparasite of Ostrinia furnacalis larvae. M. cingulum larvae repress host immune responses for survival and ingest host nutrients for development until emerging. However, most investigations focused on the mechanisms of how wasps repress the host immunity, the triggered immune responses and nutrient status altered by wasps in host are neglected. In this study, we found that parasitized O. furnacalis larvae activated fast recognition responses and produced some effectors such as lysozyme and antimicrobial peptides, along with more consumption of trehalose, glucose, and even lipid to defend against the invading M. cingulum. However, the expression of peroxidase 6 and superoxide dismutase 2 (SOD 2) was upregulated, and the messenger RNA (mRNA) levels of cellular immunity-related genes such as thioester-containing protein 2 (TEP 2) and hemocytin were also reduced, suggesting that some immune responses were selectively shut down by wasp parasitization. Taken together, all the results indicated that parasitized O. furnacalis larvae selectively activate the immune recognition response, and upregulate effector genes, but suppress ROS reaction and cellular immunity, and invest more energy to fuel certain immune responses to defend against the wasp invading. This study provides useful information for further identifying key components of the nutrition and innate immune repertoire which may shape host-parasitoid coevolutionary dynamics. © 2021 Wiley Periodicals LLC}, year = {2022}, eissn = {1520-6327} } @article{MTMT:32654950, title = {Cuticle melanization and the expression of immune-related genes in the honeybee Apis mellifera (Hymenoptera: Apidae) adult workers}, url = {https://m2.mtmt.hu/api/publication/32654950}, author = {Luz, G.F.D. and Santana, W.C. and Santos, C.G. and Medeiros, Santana L. and Serrão, J.E.}, doi = {10.1016/j.cbpb.2021.110679}, journal-iso = {COMP BIOCHEM PHYS B}, journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, volume = {257}, unique-id = {32654950}, issn = {1096-4959}, abstract = {The global decline of bee populations has several factors, including pathogens, which need overcome the insect defenses such as the physical barriers, the body cuticle and peritrophic matrix (primary defenses), as well as the secondary defenses with antimicrobial peptides (AMPs) and the enzyme lysozyme. The regulation of immune defenses according to the infection risks raises questions about the immunity of social bees due to their exposition to different pathogens pressures during the adult lifespan and tasks performed. This study evaluated the primary (body cuticle melanization, peritrophic matrix and cpr14 expression) and secondary (AMPs and lysozyme expression) defenses of the honeybee Apis mellifera workers according to the age and tasks. The expression of malvolio was used to detect precocious forage tasks outside the colony. Forager workers have higher amount of cuticular melanization in the body cuticle than nurse, but not when the age effect is retired, indicating the gradual acquisition of this compound in the integument of adult bees. The relative value of chitin in the peritrophic matrix and cpr14 mRNA are similar in all bees evaluated, suggesting that these components of primary defenses do not change according to the task and age. Differential expression of genes for AMPs in workers performing different tasks, within the same age group, indicates that the behavior stimulates expression of genes related to secondary immune defense. The expression of malvolio gene, accelerating the change in workers behavior, and those related to immune defense suggest the investment in secondary defense mechanisms when the primary defense of the body cuticle is not yet completed. © 2021 Elsevier Inc.}, keywords = {Animals; Adult; GENETICS; ARTICLE; AGE; RNA; animal; controlled study; defensin; Cell Membrane; nonhuman; animal experiment; task performance; ACTIN; Gene Expression; Gene Expression Profiling; unclassified drug; protein expression; messenger rna; complementary dna; immune response; Hymenoptera; Hymenoptera; defense mechanism; ANTIMICROBIAL PEPTIDES; ANTIMICROBIAL PEPTIDES; clinical evaluation; rna directed dna polymerase; worker; RNA extraction; lysozyme; melanin; melanin; Chitin; Chitin; deoxyribonuclease; polypeptide antibiotic agent; Bees; cuticle; RNA 16S; Differential gene expression; integument; ribosome protein; Bee; Apis mellifera; forage; Taq polymerase; forager; immune-related gene; peritrophic matrix; abaecin; cpr14 protein; hymenoptaecin; malvolio; ribosomal protein l32; cuticle melanization}, year = {2022}, eissn = {1879-1107} } @article{MTMT:33260649, title = {Emerging Evidence on Tenebrio molitor Immunity: A Focus on Gene Expression Involved in Microbial Infection for Host-Pathogen Interaction Studies}, url = {https://m2.mtmt.hu/api/publication/33260649}, author = {Petronio, Giulio Petronio and Pietrangelo, Laura and Cutuli, Marco Alfio and Magnifico, Irene and Venditti, Noemi and Guarnieri, Antonio and Abate, Getnet Atinafu and Yewhalaw, Delenasaw and Davinelli, Sergio and Di, Marco Roberto}, doi = {10.3390/microorganisms10101983}, journal-iso = {MICROORGANISMS}, journal = {MICROORGANISMS}, volume = {10}, unique-id = {33260649}, issn = {2076-2607}, abstract = {In recent years, the scientific community's interest in T. molitor as an insect model to investigate immunity and host-pathogen interactions has considerably increased. The reasons for this growing interest could be explained by the peculiar features of this beetle, which offers various advantages compared to other invertebrates models commonly used in laboratory studies. Thus, this review aimed at providing a broad view of the T. molitor immune system in light of the new scientific evidence on the developmental/tissue-specific gene expression studies related to microbial infection. In addition to the well-known cellular component and humoral response process, several studies investigating the factors associated with T. molitor immune response or deepening of those already known have been reported. However, various aspects remain still less understood, namely the possible crosstalk between the immune deficiency protein and Toll pathways and the role exerted by T. molitor apolipoprotein III in the expression of the antimicrobial peptides. Therefore, further research is required for T. molitor to be recommended as an alternative insect model for pathogen-host interaction and immunity studies.}, keywords = {innate immunity; Immunity; ANTIMICROBIAL PEPTIDES; STAPHYLOCOCCUS-AUREUS; host-pathogen interaction; GALLERIA-MELLONELLA; Tenebrio molitor; in-vivo model; mealworm beetle; APOLIPOPHORIN-III; greater wax moth; PEPTIDOGLYCAN-RECOGNITION PROTEIN}, year = {2022}, eissn = {2076-2607}, orcid-numbers = {Pietrangelo, Laura/0000-0001-7636-5581} } @article{MTMT:32424526, title = {Establishing an invertebrate Galleria mellonella greater wax moth larval model of Neisseria gonorrhoeae infection}, url = {https://m2.mtmt.hu/api/publication/32424526}, author = {Dijokaite, Aiste and Humbert, Maria Victoria and Borkowski, Emma and La Ragione, Roberto M. and Christodoulides, Myron}, doi = {10.1080/21505594.2021.1950269}, journal-iso = {VIRULENCE}, journal = {VIRULENCE}, volume = {12}, unique-id = {32424526}, issn = {2150-5594}, abstract = {Neisseria gonorrhoeae (gonococcus) causes the human sexually transmitted disease gonorrhea. Studying gonococcal pathogenesis and developing new vaccines and therapies to combat the increasing prevalence of multi-antibiotic resistant bacteria has made use of many ex vivo models based on human cells and tissues, and in vivo vertebrate models, for example, rodent, pig and human. The focus of the current study was to examine the utility of the invertebrate greater wax moth Galleria mellonella as an in vivo model of gonococcal infection. We observed that a threshold of similar to 10(6) similar to 10(7) gonococci/larva was required to kill >50% of larvae (P < 0.05), and increased toxicity correlated with reduced health index scores and pronounced histopathological changes such as increases in the total lesion grade, melanized nodules, hemocyte reaction, and multifocal adipose body degeneration. Larval death was independent of the expression of pilus or Opa protein or LOS sialylation within a single gonococcal species studied, but the model could demonstrate relative toxicity of different isolates. N. meningitidis, N. lacatamica and gonococci all killed larvae equally, but were significantly less toxic (P > 0.05) than Pseudomonas aeruginosa. Larvae primed with nontoxic doses of gonococci were more susceptible to subsequent challenge with homologous and heterologous bacteria, and larval survival was significantly reduced (P < 0.05) in infected larvae after depletion of their hemocytes with clodronate-liposomes. The model was used to test the anti-gonococcal properties of antibiotics and novel antimicrobials. Ceftriaxone (P < 0.05) protected larvae from infection with different gonococcal isolates, but not azithromycin or monocaprin or ligand-coated silver nanoclusters (P > 0.05).}, keywords = {INFECTION; Histopathology; neisseria gonorrhoeae; Galleria mellonella}, year = {2021}, eissn = {2150-5608}, pages = {1900-1920}, orcid-numbers = {Dijokaite, Aiste/0000-0002-4850-9709; Christodoulides, Myron/0000-0002-9663-4731} } @article{MTMT:31872771, title = {Influence of Helichrysum arenarium on hemocyte-mediated immune responses and phenoloxidase enzyme activity of model organism Galleria mellonella (L.)}, url = {https://m2.mtmt.hu/api/publication/31872771}, author = {Kaya, S. and Akkuş, G. and Türkdoğan, S. and Gündüz, B.}, doi = {10.1007/s42690-021-00432-8}, journal-iso = {INT J TROP INSECT SC}, journal = {INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE}, volume = {41}, unique-id = {31872771}, issn = {1742-7584}, abstract = {Helichrysum arenarium (L.) (Asterales:Asteraceae) Moench is a therapeutic plant which contains etheric oil, flavones and flavon glycosides, sterins, bitter substances and tannins having various coumarins. This plant is thought to have important characteristics such as diuretic effect, dropping stones and sand from the kidney, regulating digestive disorders, strengthening the immune system, and having antibiotic and antioxidant effects. Additionally, this plant is traditionally used in liver and biliary tract diseases and also shows anti-inflammatory and detoxifying properties. Model organism Galleria mellonella L. (Lepidoptera:Pyralidae) is an invertebrate species that is frequently used to study the effects of human pathogens and various pesticides, hormones, etc. on immune system. In our study, we examined the effect of various doses of H. arenarium on the hemocyte count and behavior of G. mellonella larvae. According to the findings obtained at the end of our study, H. arenarium caused an increase in hemocyte count with the injection of 0.25% and 0.5% doses compared to the untreated and DMSO groups. At the same time, the 0.25% and 0.5% doses showed a strong encapsulation-melanization response and an increase in phenoloxidase enzyme activity over 24 h compared to the other injected groups. Based on these results, H. arenarium extract has an anti-mitotic activity at high doses (above 0.5%). This effect may be due to the fact that the plant extract supports mitosis at a certain dose, while being toxic when exceeding it. © 2021, African Association of Insect Scientists.}, keywords = {immune system; Galleria mellonella; phenoloxidase; Encapsulation-melanization responses; Helichrysum arenarium; Hemocyte counts}, year = {2021}, eissn = {1742-7592}, pages = {2521-2528} } @article{MTMT:32424527, title = {Evolution of Toll, Spatzle and MyD88 in insects: the problem of the Diptera bias}, url = {https://m2.mtmt.hu/api/publication/32424527}, author = {Lima, Leticia Ferreira and Torres, Andre Quintanilha and Jardim, Rodrigo and Mesquita, Rafael Dias and Schama, Renata}, doi = {10.1186/s12864-021-07886-7}, journal-iso = {BMC GENOMICS}, journal = {BMC GENOMICS}, volume = {22}, unique-id = {32424527}, issn = {1471-2164}, abstract = {Background Arthropoda, the most numerous and diverse metazoan phylum, has species in many habitats where they encounter various microorganisms and, as a result, mechanisms for pathogen recognition and elimination have evolved. The Toll pathway, involved in the innate immune system, was first described as part of the developmental pathway for dorsal-ventral differentiation in Drosophila. Its later discovery in vertebrates suggested that this system was extremely conserved. However, there is variation in presence/absence, copy number and sequence divergence in various genes along the pathway. As most studies have only focused on Diptera, for a comprehensive and accurate homology-based approach it is important to understand gene function in a number of different species and, in a group as diverse as insects, the use of species belonging to different taxonomic groups is essential. Results We evaluated the diversity of Toll pathway gene families in 39 Arthropod genomes, encompassing 13 different Insect Orders. Through computational methods, we shed some light into the evolution and functional annotation of protein families involved in the Toll pathway innate immune response. Our data indicates that: 1) intracellular proteins of the Toll pathway show mostly species-specific expansions; 2) the different Toll subfamilies seem to have distinct evolutionary backgrounds; 3) patterns of gene expansion observed in the Toll phylogenetic tree indicate that homology based methods of functional inference might not be accurate for some subfamilies; 4) Spatzle subfamilies are highly divergent and also pose a problem for homology based inference; 5) Spatzle subfamilies should not be analyzed together in the same phylogenetic framework; 6) network analyses seem to be a good first step in inferring functional groups in these cases. We specifically show that understanding Drosophila's Toll functions might not indicate the same function in other species. Conclusions Our results show the importance of using species representing the different orders to better understand insect gene content, origin and evolution. More specifically, in intracellular Toll pathway gene families the presence of orthologues has important implications for homology based functional inference. Also, the different evolutionary backgrounds of Toll gene subfamilies should be taken into consideration when functional studies are performed, especially for TOLL9, TOLL, TOLL2_7, and the new TOLL10 clade. The presence of Diptera specific clades or the ones lacking Diptera species show the importance of overcoming the Diptera bias when performing functional characterization of Toll pathways.}, keywords = {EVOLUTION; innate immunity; Hexapoda; Arthropoda; TUBE; Gene family; Toll pathway; SSN; Pellino; Pelle}, year = {2021}, eissn = {1471-2164}, orcid-numbers = {Schama, Renata/0000-0003-1978-3757} } @article{MTMT:31872772, title = {Carbapenem-resistant klebsiella pneumoniae clinical isolates: In vivo virulence assessment in galleria mellonella and potential therapeutics by polycationic oligoethyleneimine}, url = {https://m2.mtmt.hu/api/publication/31872772}, author = {Mil-Homens, D. and Martins, M. and Barbosa, J. and Serafim, G. and Sarmento, M.J. and Pires, R.F. and Rodrigues, V. and Bonifácio, V.D.B. and Pinto, S.N.}, doi = {10.3390/antibiotics10010056}, journal-iso = {ANTIBIOTICS-BASEL}, journal = {ANTIBIOTICS}, volume = {10}, unique-id = {31872772}, abstract = {Klebsiella pneumoniae, one of the most common pathogens found in hospital-acquired infections, is often resistant to multiple antibiotics. In fact, multidrug-resistant (MDR) K. pneumoniae producing KPC or OXA-48-like carbapenemases are recognized as a serious global health threat. In this sense, we evaluated the virulence of K. pneumoniae KPC(+) or OXA-48(+) aiming at potential antimicrobial therapeutics. K. pneumoniae carbapenemase (KPC) and the expanded-spectrum oxacillinase OXA-48 isolates were obtained from patients treated in medical care units in Lisbon, Portugal. The virulence potential of the K. pneumonia clinical isolates was tested using the Galleria mellonella model. For that, G. mellonella larvae were inoculated using patients KPC(+) and OXA-48(+) isolates. Using this in vivo model, the KPC(+) K. pneumoniae isolates showed to be, on average, more virulent than OXA-48(+). Virulence was found attenuated when a low bacterial inoculum (one magnitude lower) was tested. In addition, we also report the use of a synthetic polycationic oligomer (L-OEI-h) as a potential antimicrobial agent to fight infectious diseases caused by MDR bacteria. L-OEI-h has a broad-spectrum antibacterial activity and exerts a significantly bactericidal activity within the first 5-30 min treatment, causing lysis of the cytoplasmic membrane. Importantly, the polycationic oligomer showed low toxicity against in vitro models and no visible cytotoxicity (measured by survival and health index) was noted on the in vivo model (G. mellonella), thus L-OEI-h is foreseen as a promising polymer therapeutic for the treatment of MDR K. pneumoniae infections. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.}, keywords = {POLYMERIZATION; ARTICLE; MOUSE; CYTOTOXICITY; human; Survival Rate; Escherichia coli; controlled study; nonhuman; animal cell; larva; Glycerol; human cell; unclassified drug; phosphatidylcholine; minimum inhibitory concentration; polycarbonate; AMIKACIN; CEFTAZIDIME; meropenem; ciprofloxacin; amoxicillin plus clavulanic acid; gentamicin; cefepime; Staphylococcus aureus; ANTIMICROBIAL ACTIVITY; Pseudomonas aeruginosa; piperacillin plus tazobactam; cefuroxime axetil; penicillin derivative; bactericidal activity; bacterial growth; streptomycin; cotrimoxazole; cefuroxime; Antibiotic resistance; bacterial virulence; hydrochloric acid; oxazoline derivative; phosphate buffered saline; CHLOROFORM; 2 propanol; matrix assisted laser desorption ionization time of flight mass spectrometry; photon correlation spectroscopy; phosphatidylglycerol; MTT assay; methicillin resistant Staphylococcus aureus; Liquid nitrogen; klebsiella pneumoniae; colistin; fosfomycin; A-549 cell line; fetal bovine serum; Klebsiella pneumoniae infection; therapeutic potential; Galleria mellonella; minimum bactericidal concentration; broth dilution; Ertapenem; Cocoon; Caterpillar; alpha cyano 4 hydroxycinnamic acid; NCTC clone 929 cell line; timentin; Toxicity assay; carbapenem resistant Klebsiella pneumoniae; Galleria mellonella infection model; KPC and OXA-48-like carbapenemases; 2 ethyl(2 oligooxazoline); aziridine derivative; linear oligoethyleneimine hydrochloride; linear oligoethyleneimine hydrochloride; polycationic oligoethyleneimine}, year = {2021}, eissn = {2079-6382} } @article{MTMT:32654954, title = {Co-formulant in a commercial fungicide product causes lethal and sub-lethal effects in bumble bees}, url = {https://m2.mtmt.hu/api/publication/32654954}, author = {Straw, E.A. and Brown, M.J.F.}, doi = {10.1038/s41598-021-00919-x}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {11}, unique-id = {32654954}, issn = {2045-2322}, abstract = {Pollinators, particularly wild bees, are suffering declines across the globe, and pesticides are thought to be drivers of these declines. Research into, and regulation of pesticides has focused on the active ingredients, and their impact on bee health. In contrast, the additional components in pesticide formulations have been overlooked as potential threats. By testing an acute oral dose of the fungicide product Amistar, and equivalent doses of each individual co-formulant, we were able to measure the toxicity of the formulation and identify the ingredient responsible. We found that a co-formulant, alcohol ethoxylates, caused a range of damage to bumble bee health. Exposure to alcohol ethoxylates caused 30% mortality and a range of sublethal effects. Alcohol ethoxylates treated bees consumed half as much sucrose as negative control bees over the course of the experiment and lost weight. Alcohol ethoxylates treated bees had significant melanisation of their midguts, evidence of gut damage. We suggest that this gut damage explains the reduction in appetite, weight loss and mortality, with bees dying from energy depletion. Our results demonstrate that sublethal impacts of pesticide formulations need to be considered during regulatory consideration, and that co-formulants can be more toxic than active ingredients. © 2021, The Author(s).}, keywords = {Animals; Body Weight; Body Weight; animal; Feeding Behavior; Feeding Behavior; drug effect; sucrose; sucrose; fungicide; gastrointestinal tract; gastrointestinal tract; pyrimidines; pyrimidine derivative; Bees; Bee; Fungicides, Industrial; Azoxystrobin; strobilurin; Strobilurins}, year = {2021}, eissn = {2045-2322} } @article{MTMT:32970995, title = {Transcriptome of the Maize Leafhopper (Dalbulus maidis) and Its Transcriptional Response to Maize Rayado Fino Virus (MRFV), Which It Transmits in a Persistent, Propagative Manner}, url = {https://m2.mtmt.hu/api/publication/32970995}, author = {Xu, Junhuan and Willman, Matthew and Todd, Jane and Kim, Kwang-Ho and Redinbaugh, Margaret G. and Stewart, Lucy R.}, doi = {10.1128/Spectrum.00612-21}, journal-iso = {MICROBIOL SPEC}, journal = {MICROBIOLOGY SPECTRUM}, volume = {9}, unique-id = {32970995}, issn = {2165-0497}, abstract = {The corn leafhopper (Dalbulus maidis) is an important vector of maize rayado fino virus (MRFV), a positive-strand RNA (+ssRNA) marafivirus which it transmits in a persistent propagative manner. The interaction of D. maidis with MRFV, including infection of the insect and subsequent transmission to new plants, is not well understood at the molecular level. To examine the leafhopper-virus interaction, a D. maidis transcriptome was assembled and differences in transcript abundance between virus-exposed and naive D. maidis were examined at two time points (4 h and 7 days) post exposure to MRFV. The D. maidis transcriptome contained 56,116 transcripts generated from 1,727,369,026 100-nt paired-end reads from whole adult insects. The transcriptome of D. maidis shared highest identity and most orthologs with the leafhopper Graminella nigrifrons (65% of transcripts had matches with E values of <10-5) versus planthoppers Sogatella furcifera (with 23% of transcript matches below the E value cutoff) and Peregrinus maidis (with 21% transcript matches below the E value cutoff), as expected based on taxonomy. D. maidis expressed genes in the Toll, Imd, and Jak/Stat insect immune signaling pathways, RNA interference (RNAi) pathway genes, prophenoloxidase-activating system pathways, and immune recognition protein-encoding genes such as peptidoglycan recognition proteins (PGRPs), antimicrobial peptides, and other effectors. Statistical analysis (performed by R package DESeq2) identified 72 transcripts at 4 h and 67 at 7 days that were significantly responsive to MRFV exposure. Genes expected to be favorable for virus propagation, such as protein synthesis-related genes and genes encoding superoxide dismutase, were significantly upregulated after MRFV exposure.IMPORTANCE The transcriptome of the corn leafhopper, D. maidis, revealed conserved biochemical pathways for immunity and discovered transcripts responsive to MRFV-infected plants at two time points, providing a basis for functional identification of genes that either limit or promote the virus-vector interaction. Compared to other hopper species and the propagative plant viruses they transmit, D. maidis shared 15 responsive transcripts with S. furcifera (to southern rice black-streaked dwarf virus [SRBSDV]), one with G. nigrifrons (to maize fine streak virus [MFSV]), and one with P. maidis (to maize mosaic virus [MMV]), but no virus-responsive transcripts identified were shared among all four hopper vector species.}, keywords = {immune response; RNA interference (RNAi); Persistent transmission; Dalbulus maidis; Benign equilibrium; Immune response signaling pathways; Maize rayado fino virus (MRFV)}, year = {2021}, eissn = {2165-0497} } @article{MTMT:31302837, title = {Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes}, url = {https://m2.mtmt.hu/api/publication/31302837}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordás, Gábor and Rusvai, Miklós and Honti, Viktor and Kolics, Balázs and Török, Tibor and Williams, Michael J and Kurucz, Judit Éva and Andó, István}, doi = {10.1016/j.dci.2020.103701}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {109}, unique-id = {31302837}, issn = {0145-305X}, abstract = {Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes.}, keywords = {Immunity; monoclonal antibody; Hemocyte; Apis mellifera; Honey bee; insect immunity}, year = {2020}, eissn = {1879-0089}, pages = {103701-103706}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Török, Tibor/0000-0002-2128-1126; Andó, István/0000-0002-4648-9396} } @article{MTMT:31467643, title = {Influence of Indole-3-Acetic Acid on Cellular Immune Responses ofGalleria mellonellaL. (Lepidoptera: Pyralidae) andPimpla turionellaeL. (Hymenoptera: Ichneumonidae) in a host-parasitoid system}, url = {https://m2.mtmt.hu/api/publication/31467643}, author = {Kaya, Serhat and Uckan, Fevzi and Er, Aylin}, doi = {10.1007/s42690-020-00190-z}, journal-iso = {INT J TROP INSECT SC}, journal = {INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE}, unique-id = {31467643}, issn = {1742-7584}, abstract = {The dietary effects of the well-known auxin member plant growth regulator, indole-3-acetic acid (IAA), on hemocyte counts and behavior of the model insectGalleria mellonellaL. (Lepidoptera: Pyralidae) and its parasitoidPimpla turionellaeL. (Hymenoptera: Ichneumonidae) were examined.In vivoandin vitroexperiments involved five treatments applied to larvae with doses of 50, 500, 1000, 5000, and 10000 ppm IAA and the controls. Total hemocyte counts increased at all applied IAA doses inG. mellonellawhereas no effect was observed inP. turionellae. According to acridine orange-ethidium bromide double staining, none of the IAA doses affected the ratio of apoptotic and mitotic cellsin vivohowever a decrease in the number of late apoptotic cells at 1000 and 5000 ppm was observed atin vitroexperiments inG. mellonella. An increase in the percentage of early apoptotic hemocytes at 1000 ppm and late apoptotic hemocytes at 500 ppm were determined inP. turionellaelarvae. IAA also increased the ratio of mitotic hemocytes in the circulation ofP. turionellaelarvae at 50, 1000, and 10000 ppm. Changes in the encapsulation rate of Sephadex A-25 beads were not significant however the increase at 5000 ppm at 4th hour in non-melanized hemocytes was recorded as compared to the control. A strong decrease in melanization at 50 ppm and an increase at 50 and 1000 ppm in non-melanized bead count at 24 h were also observed. It can be concluded that IAA is inappropriate to use in combination with microbial control agents in IPM programs due to elevated hemocyte counts of the pest.}, keywords = {APOPTOSIS; In Vitro; encapsulation; In vivo; Hemocyte; melanization; Mitotic Indicies}, year = {2020}, eissn = {1742-7592} } @article{MTMT:31467647, title = {Biased predation could promote convergence yet maintain diversity within Mullerian mimicry rings of Oreina leaf beetles}, url = {https://m2.mtmt.hu/api/publication/31467647}, author = {Kikuchi, David W. and Waldron, Samuel J. and Valkonen, Janne K. and Dobler, Susanne and Mappes, Johanna}, doi = {10.1111/jeb.13620}, journal-iso = {J EVOLUTION BIOL}, journal = {JOURNAL OF EVOLUTIONARY BIOLOGY}, volume = {33}, unique-id = {31467647}, issn = {1010-061X}, abstract = {Mullerian mimicry is a classic example of adaptation, yet Muller's original theory does not account for the diversity often observed in mimicry rings. Here, we aimed to assess how well classical Mullerian mimicry can account for the colour polymorphism found in chemically defended Oreina leaf beetles by using field data and laboratory assays of predator behaviour. We also evaluated the hypothesis that thermoregulation can explain diversity between Oreina mimicry rings. We found that frequencies of each colour morph were positively correlated among species, a critical prediction of Mullerian mimicry. Predators learned to associate colour with chemical defences. Learned avoidance of the green morph of one species protected green morphs of another species. Avoidance of blue morphs was completely generalized to green morphs, but surprisingly, avoidance of green morphs was less generalized to blue morphs. This asymmetrical generalization should favour green morphs: indeed, green morphs persist in blue communities, whereas blue morphs are entirely excluded from green communities. We did not find a correlation between elevation and coloration, rejecting thermoregulation as an explanation for diversity between mimicry rings. Biased predation could explain within-community diversity in warning coloration, providing a solution to a long-standing puzzle. We propose testable hypotheses for why asymmetric generalization occurs, and how predators maintain the predominance of blue morphs in a community, despite asymmetric generalization.}, keywords = {CONVERGENT EVOLUTION; Colour polymorphism; Aposematism; warning signal}, year = {2020}, eissn = {1420-9101}, pages = {887-898}, orcid-numbers = {Dobler, Susanne/0000-0002-0635-7719; Mappes, Johanna/0000-0002-1117-5629} } @article{MTMT:31149578, title = {Hemocytin facilitates host immune responses against Nosema bombycis}, url = {https://m2.mtmt.hu/api/publication/31149578}, author = {Ni, W. and Bao, J. and Mo, B. and Liu, L. and Li, T. and Pan, G. and Chen, J. and Zhou, Z.}, doi = {10.1016/j.dci.2019.103495}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {103}, unique-id = {31149578}, issn = {0145-305X}, abstract = {Invertebrates lack an adaptive immune response and thus are reliant on their innate immune response for eliminating invading pathogens. The innate immune responses of silkworms against the pathogen Nosema bombycis include: hemocyte aggregation, melanization, antimicrobial peptides, etc. In our current study, we discovered that a silkworm hemostasis-related protein, hemocytin, is up-regulated after Nosema bombycis infection. This novel finding lead to our hypothesis that hemocytin participates in immune responses against N. bombycis. We investigated this hypothesis by analyzing the adhesive effects of hemocytin to invading N. bombycis, and the hemocytin-mediated hemocyte aggregation and hemolymph melanization. We showed that hemocytin can adhere to the surface of N. bombycis, which facilitates the agglutination of N. bombycis and hemocytes as well as the subsequent melanization. Moreover, when we utilize RNAi technology to decrease in vivo hemocytin expression, we found that the proliferation of N. bombycis within the host significantly increased. These results support our hypothesis that hemocytin exerts pro-inflammatory effects by facilitating pathogen agglutination, along with hemocyte aggregation and melanization, to combat N. bombycis. Our study is the first to determine a function of hemocytin in innate immunity against N. bombycis. Moreover, our findings are of great importance to provide potential targets for developing novel strategy against microsporidia infection. © 2019 Elsevier Ltd}, keywords = {AGGREGATION; ARTICLE; Molecular cloning; membrane protein; priority journal; innate immunity; innate immunity; controlled study; nonhuman; animal model; animal experiment; animal cell; regulatory mechanism; Western blotting; Mass spectrometry; RNA Interference; PROTEIN FUNCTION; unclassified drug; protein expression; host resistance; immune response; blood cell; protein targeting; upregulation; melanogenesis; protein aggregation; melanization; Bombyx mori; Nosema bombycis; Nosema bombycis; hemocytin; hemocytin; pebrine}, year = {2020}, eissn = {1879-0089} } @article{MTMT:31467640, title = {Immune priming: the secret weapon of the insect world}, url = {https://m2.mtmt.hu/api/publication/31467640}, author = {Sheehan, Gerard and Farrell, Gemma and Kavanagh, Kevin}, doi = {10.1080/21505594.2020.1731137}, journal-iso = {VIRULENCE}, journal = {VIRULENCE}, volume = {11}, unique-id = {31467640}, issn = {2150-5594}, abstract = {Insects are a highly successful group of animals that inhabit almost every habitat and environment on Earth. Part of their success is due to a rapid and highly effective immune response that identifies, inactivates, and eliminates pathogens. Insects possess an immune system that shows many similarities to the innate immune system of vertebrates, but they do not possess an equivalent system to the antibody-mediated adaptive immune response of vertebrates. However, some insect do display a process known as immune priming in which prior exposure to a sublethal dose of a pathogen, or pathogen-derived material, leads to an elevation in the immune response rendering the insect resistant to a subsequent lethal infection a short time later. This process is mediated by an increase in the density of circulating hemocytes and increased production of antimicrobial peptides. Immune priming is an important survival strategy for certain insects while other insects that do not show this response may have colony-level behaviors that may serve to limit the success of pathogens. Insects are now widely used as in vivo models for studying microbial pathogens of humans and for assessing the in vivo efficacy of antimicrobial agents. Knowledge of the process of immune priming in insects is essential in these applications as it may operate and augment the perceived in vivo antimicrobial activity of novel compounds.}, keywords = {INFECTION; Immunity; priming; In vivo model; Galleria larvae}, year = {2020}, eissn = {2150-5608}, pages = {238-246}, orcid-numbers = {Sheehan, Gerard/0000-0003-1922-4103} } @article{MTMT:31467645, title = {Transcriptome characterization and gene expression analysis related to immune response in Gynaephora qinghaiensis pupae}, url = {https://m2.mtmt.hu/api/publication/31467645}, author = {Wang, Hai-Zhen and Zhong, Xin and Zhang, Gu-Ren and Liu, Xin and Gu, Li}, doi = {10.1016/j.aspen.2020.01.013}, journal-iso = {J ASIA PAC ENTOMOL}, journal = {JOURNAL OF ASIA PACIFIC ENTOMOLOGY}, volume = {23}, unique-id = {31467645}, issn = {1226-8615}, abstract = {Gynaephora qinghaiensis is a pest on the Qinghai-Tibet Plateau (QTP) that has led to substantial destruction of grassland vegetation. Its pupae are also natural hosts of parasitic wasp. Sexual dimorphism in immune responses is prevalent in vertebrates and invertebrates. However, sexual dimorphism in immune responses of insects, particularly G. qinghaiensis, is poorly understood at the transcriptional level. Here, we performed transcriptome sequencing in male and female pupae of G. qinghaiensis. A total of 118,357,040 clean reads were obtained and assembled into 114,944 unigenes. To explore the difference of immune responses in pupae of both sexes (male and female) of G. qinghaiensis. based on transcriptional level, we characterized the expression profiles of candidate transcripts in the two sexes of G. qinghaiensis. A total of 3,469 unigenes (1,888 up-regulated and 1,581 down-regulated genes) were differentially expressed in pupae of both sexes. Among the differentially expressed genes (DEGs), 263 unigenes related to immune responses were found in the two sexes in G. qinghaiensis; of these, 202 were up-regulated and 61 were down-regulated in the female pupae compared to male pupae. This indicates there were some differences in the expression of immune-related genes between male and female G. qinghaiensis pupae. Therefore, we speculated that the differences in immune responses also likely exsited in pupae of both sexes. Our report provides a valuable genomic resource for further studies of Gynaephora and improves our understanding of the molecular mechanisms underlying immunological differences between male and female insects.}, keywords = {Transcriptome; RNA-Seq; immune responses; Gynaephora}, year = {2020}, eissn = {1876-7990}, pages = {458-469} } @article{MTMT:32006486, title = {The greater wax moth Galleria mellonella: Biology and use in immune studies}, url = {https://m2.mtmt.hu/api/publication/32006486}, author = {Wojda, I. and Staniec, B. and Sułek, M. and Kordaczuk, J.}, doi = {10.1093/femspd/ftaa057}, journal-iso = {PATHOG DIS}, journal = {PATHOGENS AND DISEASE}, volume = {78}, unique-id = {32006486}, issn = {2049-632X}, abstract = {The greater wax moth Galleria mellonella is an invertebrate that is increasingly being used in scientific research. Its ease of reproduction, numerous offspring, short development cycle, and finally, its known genome and immune-related transcriptome provide a convenient research model for investigation of insect immunity at biochemical and molecular levels. Galleria immunity, consisting of only innate mechanisms, shows adaptive plasticity, which has recently become the subject of intensive scientific research. This insect serves as a mini host in studies of the pathogenicity of microorganisms and in vivo tests of the effectiveness of single virulence factors as well as new antimicrobial compounds. Certainly, the Galleria mellonella species deserves our attention and appreciation for its contribution to the development of research on innate immune mechanisms. In this review article, we describe the biology of the greater wax moth, summarise the main advantages of using it as a model organism and present some of the main techniques facilitating work with this insect © 2020 The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.}, keywords = {host-pathogen interaction; Life history; Galleria mellonella; insect immunity; insect model organism}, year = {2020}, eissn = {2049-632X} } @article{MTMT:31685791, title = {Caste and age-related changes in circulatory hemocytes of honey bee, Apis mellifera anatolica (Hymenoptera: Apidae)}, url = {https://m2.mtmt.hu/api/publication/31685791}, author = {Yelkovan, Sedat and Arikan, Huseyin and Cakici, Ozlem}, doi = {10.1080/00218839.2020.1834768}, journal-iso = {J APICULT RES}, journal = {JOURNAL OF APICULTURAL RESEARCH}, unique-id = {31685791}, issn = {0021-8839}, abstract = {In recent years, there has been a great concern about increased honey bee losses and this phenomenon has turned into a global issue. Therefore, immune system research on honey bees gained importance. Hemocytes are an important part of the immune system. But there is a limited study about hemocyte types of honey bees. From this point of view, a detailed study was performed on hemocyte types of honey bee Apis mellifera anatolica was described in larval (3rd, 4th and 5th larval instars), prepupal, pupal, and adult stages of all castes including worker, drone, and queen. According to light microscopic examinations, prohemocyte, plasmatocyte, granulocyte, adipohemocyte and oenocytoid were determined in all castes. Prohemocytes are usually rounded cells with a large nucleus and small cytoplasmic area. Plasmatocytes are spherical or oval cells. Granulocytes are generally spherical cells with dense granules. Adipohemocytes are usually spherical cells having numerous lipid droplets within their cytoplasm. Oenocytoides are usually spherical cells with small and spherical nucleus centrally located. Also, we statistically compared the size of hemocyte types among these castes during metamorphosis. According to our measurements, the queen generally had the biggest hemocytes in comparison to the developmental stages in other castes. While statistically significant differences in the sizes of hemocyte types were mainly detected among castes of the pupal stage, there were no statistically significant differences in hemocyte sizes among castes of adult honey bees. In view of these findings, it may be stated that hemocyte profiles of A. m. anatolica vary based on the developmental stages of castes.}, keywords = {innate immunity; Honey bee; apiculture; Apis mellifera anatolica; hemocyte types}, year = {2020}, eissn = {2078-6913} } @article{MTMT:31872761, title = {Isolation and diagnostic Bacillus thuringiensis tenebrionis pathogenesis for insects from date palm stemborerlarva(JebusaeahammersChmidt:Coleoptera:Cearmbyicidae)}, url = {https://m2.mtmt.hu/api/publication/31872761}, author = {Al-Saeedi, H.M. and Al-Jassany, R.F.}, journal-iso = {PLANT ARCH}, journal = {PLANT ARCHIVES}, volume = {19}, unique-id = {31872761}, issn = {0972-5210}, abstract = {The aim of this study to isolate and diagnose pathogen bacteria that caused date palm stem borer larva.economic important for date palm in Iraq. The process of diagnostic depend on morphological properties and biochemical tests in addition it biophysical specialties and polymerase chain reaction (PCR) for Cry3 gene, the pictures when taken by transmission electron microscope (TEM). The results of different tests when fourel this bacteria responsible for larvae death Bacillus thuringiensis subsp. tenebrionis. through compatibility of nucleotide based sequences in 99% between molecular diagnostic results and Cry gene analysis Bacillus thuringiensis serovar morrisoni BBSC 4AA1 which NCBI bank genes. The isolated bacteria B. t. tenebrionis caused 100% mortality after 2 day in the first and second larvae instare of date palm stem borer Jebusaea hammerschmidt and 100% mortality in the last instar of the larvae after 4 days.This bacteria B. t. tenebrionis was recorded the first time on larvae of date palm stem borer. © 2019 Plant Archives. All rights reserved.}, keywords = {INSECTS; PALM; Bacillus thuringiensis tenebrionis}, year = {2019}, eissn = {2581-6063}, pages = {3331-3337} } @article{MTMT:31580431, title = {Identification of a novel humoral antifungal defense molecule in the hemolymph of tasar silkworm Antheraea mylitta}, url = {https://m2.mtmt.hu/api/publication/31580431}, author = {Chakraborty, Mousumi and Mandal, Santi M. and Basak, Amit and Ghosh, Ananta K.}, doi = {10.1016/j.bbrc.2019.08.143}, journal-iso = {BIOCHEM BIOPH RES CO}, journal = {BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS}, volume = {519}, unique-id = {31580431}, issn = {0006-291X}, abstract = {Humoral defenses are the major components of insect innate immune system that include the production of several soluble effector molecules from fat body and hemocytes, and released in to the hemolymph upon microbial infection. Hemolymph was collected from the fungal immunized fifth instar larvae of tasar silkworm, Antheraea mylitta, extracted with a mixture of solvent (methanol/glacial acetic acid/water) and fractionated through RP-HPLC. Several fractions were collected, lyophilized and their antifungal activity was tested against Candida albicans. Only the fraction showing strong antifungal activity was further purified via gel filtration chromatography and the purity of active compound was confirmed by thin layer chromatography which showed only single spot after staining with ninhydrin. The molecular mass of this purified compound was determined by high resolution mass spectrometry as 531 Da and analysis of H-1 and C-13 NMR spectral data along with mass fragmentation pattern indicated the probable structure of the isolated compound as symmetric bis-decanoate derivative. Scanning electron microscopic study revealed that the compound degraded fungal cell wall leading to its lysis and may be the major target for its antifungal activity. These results indicate that presence of this compound in the hemolymph of A. mylitta provides defense against fungal infection. (C) 2019 Elsevier Inc. All rights reserved.}, keywords = {Candida albicans; Hemolymph; antifungal compound; Antheraea mylitta; Bis-decanoic acid}, year = {2019}, eissn = {1090-2104}, pages = {121-126} } @article{MTMT:30906927, title = {Diet modulates the relationship between immune gene expression and functional immune responses}, url = {https://m2.mtmt.hu/api/publication/30906927}, author = {Cotter, Sheena C. and Reavey, Catherine E. and Tummala, Yamini and Randall, Joanna L. and Holdbrook, Robert and Ponton, Fleur and Simpson, Stephen J. and Smith, Judith A. and Wilson, Kenneth}, doi = {10.1016/j.ibmb.2019.04.009}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {109}, unique-id = {30906927}, issn = {0965-1748}, abstract = {Nutrition is vital to health and the availability of resources has long been acknowledged as a key factor in the ability to fight off parasites, as investing in the immune system is costly. Resources have typically been considered as something of a "black box", with the quantity of available food being used as a proxy for resource limitation. However, food is a complex mixture of macro- and micronutrients, the precise balance of which determines an animal's fitness. Here we use a state-space modelling approach, the Geometric Framework for Nutrition (GFN), to assess for the first time, how the balance and amount of nutrients affects an animal's ability to mount an immune response to a pathogenic infection.Spodoptera littoralis caterpillars were assigned to one of 20 diets that varied in the ratio of macronutrients (protein and carbohydrate) and their calorie content to cover a large region of nutrient space. Caterpillars were then handled or injected with either live or dead Xenorhabdus nematophila bacterial cells. The expression of nine genes (5 immune, 4 non-immune) was measured 20 h post immune challenge. For two of the immune genes (PPO and Lysozyme) we also measured the relevant functional immune response in the hemolymph. Gene expression and functional immune responses were then mapped against nutritional intake.The expression of all immune genes was up-regulated by injection with dead bacteria, but only those in the IMD pathway (Moricin and Relish) were substantially up-regulated by both dead and live bacterial challenge. Functional immune responses increased with the protein content of the diet but the expression of immune genes was much less predictable.Our results indicate that diet does play an important role in the ability of an animal to mount an adequate immune response, with the availability of protein being the most important predictor of the functional (physiological) immune response. Importantly, however, immune gene expression responds quite differently to functional immunity and we would caution against using gene expression as a proxy for immune investment, as it is unlikely to be reliable indicator of the immune response, except under specific dietary conditions.}, keywords = {RESISTANCE; TOLERANCE; BACTERIA; INSECT; Immunity; Spodoptera; DIET; host-pathogen interaction; XENORHABDUS; Geometric framework; Nutritional ecology}, year = {2019}, eissn = {1879-0240}, pages = {128-141}, orcid-numbers = {Ponton, Fleur/0000-0001-7156-3896; Wilson, Kenneth/0000-0001-5264-6522} } @article{MTMT:30906926, title = {Potential geo-tracing tool for migrant insects by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE}, url = {https://m2.mtmt.hu/api/publication/30906926}, author = {El Sheikha, Aly Farag and Menozzi, Philippe}, doi = {10.1007/s42690-019-00002-z}, journal-iso = {INT J TROP INSECT SC}, journal = {INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE}, volume = {39}, unique-id = {30906926}, issn = {1742-7584}, abstract = {Insect movement in the landscape remains often poorly known and in some cases does not make it possible to understand the role of the different cultivated and wild habitats in the dynamics of useful and pest insects. Insects are among the greatest pests of agriculture, horticulture and forestry worldwide, inflicting damage and economic costs both directly and by transmitting plant viruses. There is a need for tracking the migrant insects in agroecosystems through space and time to establish their migation. Furthermore, tracing the origin of pest insects allows to design more rapid, efficient and environment-friendly control systems (less use of insecticides). Tracking insects can also help us better understanding their biology (e.g. insect population dynamics, geo-traceability, feeding behavior and other ecological interactions). However. tracking insects presents a considerable challenge as they are often small, cryptic and highly mobile organisms. The most common methods of analysis currently proposed (microsatellite markers, stable isotopes) do not allow for the moment to determine their geographical origin. The ecological niche occupied by the insects highly influences the bacterial communities associated with the insects. Hence understanding the diversity of bacterial communities could be an option to trace the orgins of the pest. This paper presents two new protocols used to extract bacterial DNA from the Cotton Bollworm. Helicoverpa armigera and the analysis of this DNA by PCR-DGGE. This promising method is proposed as a new traceability tool which provides insects with a unique biological barcode and makes it possible to trace back the insects to their original location.}, keywords = {BACTERIAL COMMUNITY; PCR-DGGE; Helicoverpa armigera; insect pests; Geo-location fingerprinting; Migrant insects; Biological barcoding}, year = {2019}, eissn = {1742-7592}, pages = {9-16} } @{MTMT:31872769, title = {Entomopathogenic nematodes in the biological control of insect pests with reference to insect immunity}, url = {https://m2.mtmt.hu/api/publication/31872769}, author = {Istkhar, . and Chaubey, A.K. and Garg, A.P.}, booktitle = {Plant Biotic Interactions: State of the Art}, doi = {10.1007/978-3-030-26657-8_11}, unique-id = {31872769}, abstract = {Insects are the successfully living arthropods on earth and challenging humans for food and shelter from several decades. As agricultural pests, insects cause heavy losses to crops and reduce the quality and quantity of human food. Failure of chemical pesticides and development of resistance of insects have become the major problem for researchers and are challenging mankind to search out for better solutions. Biological control management has been found as an emerging key of success, and in this context, entomopathogenic nematodes are in concern as potential biological control agents. These nematodes parasitize and enter the body of insect host and kill them quickly without the development of any resistance and failure. Several species of the entomopathogenic nematode genera Steinernema and Heterorhabditis have been identified around the world, and few of them have been commercialized for the biomanagement of several insect pests. The killing capabilities of these nematodes were enhanced severalfold by their symbiont bacteria of the genera Xenorhabdus and Photorhabdus which reside in the alimentary canal of these rhabditid nematodes. The symbiont bacteria produce severe toxins which ultimately results in the death of insect hosts. For the successful establishment of a biological species as biocontrol agent of insects, it is necessary to study the immune responses of host and defensive mechanisms of the parasites. Insects comprise innate immune system where cellular and humoral components combat with the invading pathogens. When entomopathogenic nematodes enter in the haemocoel of insect’s body, both the immune components are triggered and try to evade pathogenicity of invading nematode-bacterium complexes. The mechanisms of nematode immunomodulation display the variation of modulatory approaches developed by different types of parasitic nematodes to cripple the host immune responses. Both the nematode and bacteria produce chemical factors which protect them from the insect’s immune system attack. The cellular and humoral responses are avoided and broken by toxins produced by nematode-bacterium complexes included different proteins producing immunodeficiency in insects. These nematodes were found to be safe to the environments and soil flora and fauna and also have exemption from one area to another by several countries. © Springer Nature Switzerland AG 2019.}, keywords = {IMMUNODEFICIENCY; HETERORHABDITIS; Steinernema; immune responses; Biological control management; Insect’s parasite}, year = {2019}, pages = {181-209} } @article{MTMT:31872770, title = {Use of Greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for Analysis of Bacterial Pathogenesis}, url = {https://m2.mtmt.hu/api/publication/31872770}, author = {Kamal, F. and Peters, D.L. and McCutcheon, J.G. and Dunphy, G.B. and Dennis, J.J.}, doi = {10.1007/978-1-4939-8940-9_13}, journal-iso = {METHODS MOL BIOL}, journal = {METHODS IN MOLECULAR BIOLOGY}, volume = {1898}, unique-id = {31872770}, issn = {1064-3745}, abstract = {Alternative infection models of bacterial pathogenesis are useful because they reproduce some of the disease characteristics observed in higher animals. Insect models are especially useful for modeling bacterial infections, as they are inexpensive, generally less labor-intensive, and more ethically acceptable than experimentation on higher organisms. Similar to animals, insects have been shown to possess innate immune systems that respond to pathogenic bacteria. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.}, keywords = {Animals; Humans; GENETICS; BACTERIA; INSECTS; Pathogenesis; Pathogenesis; human; animal; Virulence; Virulence; innate immunity; controlled study; nonhuman; animal model; animal experiment; larva; larva; larva; microbiology; pathogenicity; Disease Models, Animal; MOTH; MOTHS; disease model; disease model; bacterium; bacterial infection; bacterial infection; bacterial strain; virulence factor; Bacterial Infections; bacterial virulence; colony forming unit; bacterium mutant; bacterial cell; Animal Testing Alternatives; LARVAE; Bacterial Load; procedures; Galleria mellonella; Galleria mellonella; infection model; animal testing alternative; Wax worm}, year = {2019}, eissn = {1940-6029}, pages = {163-171} } @article{MTMT:30906929, title = {Diseases of insects in European production systems: Diagnosis, prevention and management}, url = {https://m2.mtmt.hu/api/publication/30906929}, author = {Lecocq, Antoine and Jensen, Annette Bruun and Eilenberg, Jurgen}, doi = {10.2376/0005-9366-18061}, journal-iso = {BERL MUNCH TIERARZTL}, journal = {BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT}, volume = {132}, unique-id = {30906929}, issn = {0005-9366}, abstract = {The rapid growth of insect production for food and feed in Europe in the 21st century is accompanied by a need for better tools to assist producers in the diagnosis, prevention and management of insect diseases, which may threaten productivity. In this paper we introduce the main pathogens (viruses, bacteria, fungi) associated with insect production in European facilities as well as their diagnosis. Furthermore, we provide an introduction to insect immunity and we give guidelines for the prevention and management of diseases in order to minimize risk and losses to the producers. We describe our ongoing research on probiotics as a tool to enhance insect health. More European collaborations on diagnosis and management of insect diseases are needed, and our team welcomes insect producers, veterinarians, food microbiologists etc. to develop such collaboration.}, keywords = {FOOD; PATHOGENS; FEED; LIVESTOCK; Animal health}, year = {2019}, eissn = {1439-0299}, pages = {245-250} } @article{MTMT:30906930, title = {Immune Response of Galleria mellonella against Human Fungal Pathogens}, url = {https://m2.mtmt.hu/api/publication/30906930}, author = {Trevijano-Contador, Nuria and Zaragoza, Oscar}, doi = {10.3390/jof5010003}, journal-iso = {J FUNGI}, journal = {JOURNAL OF FUNGI}, volume = {5}, unique-id = {30906930}, abstract = {In many aspects, the immune response against pathogens in insects is similar to the innate immunity in mammals. This has caused a strong interest in the scientific community for the use of this model in research of host-pathogen interactions. In recent years, the use of Galleria mellonella larvae, an insect belonging to the Lepidoptera order, has emerged as an excellent model to study the virulence of human pathogens. It is a model that offers many advantages; for example, it is easy to handle and establish in every laboratory, the larvae have a low cost, and they tolerate a wide range of temperatures, including human temperature 37 degrees C. The immune response of G. mellonella is innate and is divided into a cellular component (hemocytes) and humoral component (antimicrobial peptides, lytic enzymes, and peptides and melanin) that work together against different intruders. It has been shown that the immune response of this insect has a great specificity and has the ability to distinguish between different classes of microorganisms. In this review, we delve into the different components of the innate immune response of Galleria mellonella, and how these components manifest in the infection of fungal pathogens including Candida albicans, Aspergillus fumigatus, Cryptococcus neoformans, and Histoplasma capsulatum.}, keywords = {innate immunity; FUNGAL PATHOGENS; Cellular response; Galleria mellonella; humoral response}, year = {2019}, eissn = {2309-608X}, orcid-numbers = {Zaragoza, Oscar/0000-0002-1581-0845} } @article{MTMT:30906928, title = {Characterization of fat body cells at different developmental stages of Culex pipiens}, url = {https://m2.mtmt.hu/api/publication/30906928}, author = {Turgay-Izzetoglu, Gamze and Gulmez, Mehtap}, doi = {10.1016/j.acthis.2019.04.002}, journal-iso = {ACTA HISTOCHEM}, journal = {ACTA HISTOCHEMICA}, volume = {121}, unique-id = {30906928}, issn = {0065-1281}, abstract = {The fat body, originates from mesoderm, has many metabolic functions which changes as the embryonic development of the insect progresses. It plays an important role in the intermediate metabolism and in the metabolism of proteins, lipids and carbohydrates. It has roles in synthesis, absorption and storage of nutrients from hemolymph. It is also responsible for the production of immunological system components, antibacterial compounds and blood clotting proteins. The most common type of fat body cells are trophocytes (the basic cells of the fat body) and oenocytes are found associated with the fat body. In this study, it is aimed at determining the cell types contained in the fat body of Culex pipiens at different developmental stages as well as identifying the molecules such as carbohydrate, protein and lipid contained in each of these cells. Knowing the regional distribution of the fat body cells and the concentration of its content at each developmental stage is important in understanding the process related to its physiology and it may help in fighting against the pest C. pipiens, which is a vector species for many contagious diseases observed in humans and other species.To achieve our goal, we have employed different histochemical techniques (fixatives and staining methods) for staining C. pipiens preparates of different developmental stages and analyzed the structure of the fat body, its distribution, its cell types and the macromolecular contents of the cells. We only observed trophocytes and oenocytes as fat body components in C. pipiens. The trophocytes had all the three macromolecules (lipids, proteins, carbohydrates) in the cytoplasm varying in concentration between the different regions and different stages. The oenocytes were observed below the integument as well as between the muscles in the larvae of Culex pipiens. They were present either as single cells or in clusters and also varied in size. Their cytoplasm was stained strongly for proteins when bromophenol blue staining was applied, but it was rather heterogeneous due to the lipid inclusions. On the contrary, oenocytes were not observed among the adult C. pipiens preparations.}, keywords = {INSECT; Histochemistry; fat body; Culex pipiens; Trophocyte; Oenocyte}, year = {2019}, eissn = {1618-0372}, pages = {460-471} } @article{MTMT:30906925, title = {Host-pathogen interactions upon the first and subsequent infection of Galleria mellonella with Candida albicans}, url = {https://m2.mtmt.hu/api/publication/30906925}, author = {Vertyporokh, Lidiia and Kordaczuk, Jakub and Mak, Pawel and Hulas-Stasiak, Monika and Wojda, Iwona}, doi = {10.1016/j.jinsphys.2019.103903}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {117}, unique-id = {30906925}, issn = {0022-1910}, abstract = {Insects are able to develop enhanced resistance in response to repeated infection. This phenomenon is called immune priming. In this work, so-called "primed" Galleria mellonella larvae were re-infected with a lethal dose of Candida albicans 48 h after injection of a non-lethal dose, while "non-primed" larvae were infected only with a lethal dose. The increased resistance of the primed larvae correlated with a slower rate of body colonisation by the fungus. Changes in the protein profiles were detected in the whole hemolymph of the primed insects. The analysis of low-molecular weight proteins and peptides obtained with the use of three different organic solvents and comparative quantitative HPLC analysis thereof showed that the primed larvae did not have higher amounts of any infection-inducible polypeptides than the non-primed larvae. Moreover, electrophoresis of low-molecular weight polypeptides revealed an even lower level of immune-induced peptides in the primed larvae than in the non-primed ones.Furthermore, the defence activity of larval hemolymph, i.e. the antifungal, antibacterial, and lysozyme-type activity, was up-regulated in the primed larvae at the time of re-infection and, consequently, at the early time points after the infection with the lethal dose. Twenty four hours after the infection, these parameters were equally high in the non-primed and primed larvae. Accordingly, at the time of the injection of the lethal dose, certain immune-inducible genes were up-regulated. However, 24 h after the infection with the lethal dose, their expression in both groups was incomparably higher than at the time of the infection and, in most cases, it was as high in the primed larvae as in the non-primed ones. We found that only anti yeast-like activity was enhanced 24 h after the re-infection. This correlated with results obtained by testing the priming effect in heterologous systems: the primed animals did not exhibit higher resistance to the other pathogens tested.}, keywords = {Candida albicans; immune priming; Galleria mellonella; Insect immune response}, year = {2019}, eissn = {1879-1611} } @article{MTMT:30781647, title = {Quantitative proteomics analysis provides insight into the biological role of Hsp90 in BmNPV infection in Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/30781647}, author = {Wu, Ping and Shang, Qi and Huang, Haoling and Zhang, Shaolun and Zhong, Jinbo and Hou, Qirui and Guo, Xijie}, doi = {10.1016/j.jprot.2019.103379}, journal-iso = {J PROTEOMICS}, journal = {JOURNAL OF PROTEOMICS}, volume = {203}, unique-id = {30781647}, issn = {1874-3919}, abstract = {Heat shock protein 90, an essential chaperone responsible for the correct maturation of key proteins, has been confirmed to facilitate Bombyx mori nucleopolyhedrovirus (BmNPV) proliferation but the mechanism is not clear. In this study, we use quantitative proteomics analysis to investigate the mechanism of Hsp90 in BmNPV replication. In total, 195 differentially expressed proteins (DEPs) were identified with 136 up-regulated proteins and 59 down-regulated proteins. The protein expression level of small heat shock proteins, immune-related proteins, cellular DNA repair-related proteins and zinc finger proteins is significantly enhanced while that of protein kinases is declined. KEGG pathway analysis reveals that DEPs are involved in longevity regulating pathway, mTOR signaling pathway, FoxO signaling pathway and Toll and Imd signaling pathway. Based on the DEPs results, we speculate that inhibition of Hsp90 suppresses the BmNPV infection may because it could not only stimulate the host innate immune, induce small heat shock proteins expression to maintain the cellular proteostasis but activate host transcription factors to bind to virus DNA or protein and subsequently hinder virus replication. The results will help understand the roles of Hsp90 in BmNPV infection and shed light on new clue to illustrate the molecular mechanism of silkworm-virus interaction.Significance: This is the first report on Hsp90 roles in BmNPV infection based on proteomic analysis. Our findings may provide new clue and research orientation to illustrate the molecular mechanism of silkworm-virus interaction and a set of BmHsp90 candidate clients, which may involve in BmNPV infection in BmN cells.}, keywords = {host-pathogen interaction; Silkworm; differentially expressed proteins; BmNPV; Hsp90 inhibitior; Quantitative proteomics analysis}, year = {2019}, eissn = {1876-7737} } @book{MTMT:31872766, title = {Precision medicine and the reinvention of human disease}, url = {https://m2.mtmt.hu/api/publication/31872766}, isbn = {9780128143933}, author = {Berman, J.J.}, doi = {10.1016/C2017-0-01874-8}, publisher = {Elsevier B.V.}, unique-id = {31872766}, abstract = {Despite what you may have read in the popular press and in social media, Precision Medicine is not devoted to finding unique treatments for individuals, based on analyzing their DNA. To the contrary, the goal of Precision Medicine is to find general treatments that are highly effective for large numbers of individuals who fall into precisely diagnosed groups. We now know that every disease develops over time, through a sequence of defined biological steps, and that these steps may differ among individuals, based on genetic and environmental conditions. We are currently developing rational therapies and preventive measures, based on our precise understanding of the steps leading to the clinical expression of diseases. Precision Medicine and the Reinvention of Human Disease explains the scientific breakthroughs that have changed the way that we understand diseases, and reveals how medical scientists are using this new knowledge to launch a medical revolution. © 2018 Elsevier Inc. All rights reserved.}, year = {2018}, pages = {1-398} } @article{MTMT:30512089, title = {Fungal infection causes changes in the number, morphology and spreading ability of Galleria mellonella haemocytes}, url = {https://m2.mtmt.hu/api/publication/30512089}, author = {Bogus, Mieczyslawa I. and Ligeza-Zuber, Marta and Polanska, Marta A. and Mosiewicz, Maciej and Wloka, Emilia and Sobocinska, Martyna}, doi = {10.1111/phen.12246}, journal-iso = {PHYSIOL ENTOMOL}, journal = {PHYSIOLOGICAL ENTOMOLOGY}, volume = {43}, unique-id = {30512089}, issn = {0307-6962}, abstract = {The most effective and important strategy in the insect immune response is based on cellular reactions incorporating haemocytes. The present study uses Galleria mellonella (Lepidoptera: Pyralidae) as a host to study the pathogenesis caused by the entomopthoralean fungus Conidiobolus coronatus (Entomophthorales). Five types of haemocytes with different morphologies and behaviour are observed in the haemolymph of G. mellonella: granulocytes (GRs), plasmatocytes (PLs), spherulocytes (SPs), oenocytes (OEs) and prohaemocytes (PRs). During in vitro cultivation, three morphological subtypes of PLs are distinguished: flattened PLs, sun-like PLs and oval PLs. In fresh smears of haemolymph observed under phase-contrast microscopy, only flattened PLs are identified. No morphological changes are observed between fresh smears and in vitro cultures for GR, OE, SP and PR. Haemocytes cultured in vitro form a cellular network composed of PLs and GRs. Changes in the numbers, morphology and behaviour of haemocytes induced by fungal infection are compared with those observed in normally-developing untreated larvae. Infection results in a significant drop in the number of haemocyte types. Fresh smears of haemocytes from mycosed larvae reveal malformed OEs, vacuolized PLs and GRs, as well as PLs with apoptotic blebs. Haemocytes from mycosed larvae incubated in vitro look similar, with degranulated GRs and vacuolized PLs forming microaggregations, as well as deformed OEs; only the SPs remain unharmed. Fungal infection impairs the ability of haemocytes to attach and spread on the culture dish. The actin cytoskeleton of haemocytes from mycosed larvae appear disorganized.}, keywords = {mycosis; PHALLOIDIN; F-ACTIN; Galleria mellonella; haemocytes; Conidiobolus coronatus}, year = {2018}, eissn = {1365-3032}, pages = {214-226} } @article{MTMT:30512086, title = {Does the Pollen Diet Influence the Production and Expression of Antimicrobial Peptides in Individual Honey Bees?}, url = {https://m2.mtmt.hu/api/publication/30512086}, author = {Danihlik, Jiri and Skrabisova, Maria and Lenobel, Rene and Sebela, Marek and Omar, Eslam and Petrivalsky, Marek and Crailsheim, Karl and Brodschneider, Robert}, doi = {10.3390/insects9030079}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {9}, unique-id = {30512086}, abstract = {We investigated the importance of protein nutrition for honey bee immunity. Different protein diets (monofloral pollen of Helianthus spp., Sinapis spp., Asparagus spp., Castanea spp., a mixture of the four different pollen and the pollen substitute Feedbee(TM)) were fed to honey bees in cages ad libitum. After 18 days of feeding, apidaecin 1 isoforms concentration in the thorax were measured using nanoflow liquid chromatography coupled with mass spectrometry. Expression levels of genes, coding for apidaecins and abaecin in the abdomen were determined using quantitative PCR. The results indicate that protein-containing nutrition in adult worker honey bees can trigger certain metabolic responses. Bees without dietary protein showed lower apidaecin 1 isoforms concentrations. The significantly lowest concentration of apidaecin 1 isoforms was found in the group that was fed no pollen diet when compared to Asparagus, Castanea, Helianthus, and Sinapis pollen or the pollen supplement FeedBee(TM). Expression levels of the respective genes were also affected by the protein diets and different expression levels of these two antimicrobial peptides were found. Positive correlation between concentration and gene expression of apidaecins was found. The significance of feeding bees with different protein diets, as well as the importance of pollen nutrition for honey bee immunity is demonstrated.}, keywords = {POLLEN; Gene Expression; Dietary Proteins; APIDAECIN; Apis mellifera; abaecin}, year = {2018}, eissn = {2075-4450}, orcid-numbers = {Danihlik, Jiri/0000-0002-6936-1766; Lenobel, Rene/0000-0001-7387-0056; Sebela, Marek/0000-0001-9835-1375; Brodschneider, Robert/0000-0002-2535-0280} } @article{MTMT:30512084, title = {An Invertebrate Host to Study Fungal Infections, Mycotoxins and Antifungal Drugs: Tenebrio molitor}, url = {https://m2.mtmt.hu/api/publication/30512084}, author = {de Souza, Patricia Canteri and Caloni, Carla Custodio and Wilson, Duncan and Almeida, Ricardo Sergio}, doi = {10.3390/jof4040125}, journal-iso = {J FUNGI}, journal = {JOURNAL OF FUNGI}, volume = {4}, unique-id = {30512084}, abstract = {Faced with ethical conflict and social pressure, researchers have increasingly chosen to use alternative models over vertebrates in their research. Since the innate immune system is evolutionarily conserved in insects, the use of these animals in research is gaining ground. This review discusses Tenebrio molitor as a potential model host for the study of pathogenic fungi. Larvae of T. molitor are known as cereal pests and, in addition, are widely used as animal and human feed. A number of studies on mechanisms of the humoral system, especially in the synthesis of antimicrobial peptides, which have similar characteristics to vertebrates, have been performed. These studies demonstrate the potential of T. molitor larvae as a model host that can be used to study fungal virulence, mycotoxin effects, host immune responses to fungal infection, and the action of antifungal compounds.}, keywords = {HEMOCYTES; innate immunity; MYCOTOXINS; Fungal infection; alternative method of infection; Candida spp. Cryptococcus spp.; invertebrate host model; mealworm; tenecin}, year = {2018}, eissn = {2309-608X} } @article{MTMT:27324962, title = {Increased survival of honeybees in the laboratory after simultaneous exposure to low doses of pesticides and bacteria}, url = {https://m2.mtmt.hu/api/publication/27324962}, author = {Dickel, Franziska and Munch, Daniel and Amdam, Gro Vang and Mappes, Johanna and Freitak, Dalial}, doi = {10.1371/journal.pone.0191256}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {13}, unique-id = {27324962}, issn = {1932-6203}, year = {2018}, eissn = {1932-6203} } @article{MTMT:31872767, title = {Insect-mediated vectoring of Pseudomonas syringae pv. actinidiae}, url = {https://m2.mtmt.hu/api/publication/31872767}, author = {Donati, I. and Cellini, A. and Buriani, G. and Mauri, S. and Spinelli, F.}, doi = {10.17660/ActaHortic.2018.1218.36}, journal-iso = {ACTA HORTICULTURAE}, journal = {ACTA HORTICULTURAE: TECHNICAL COMMUNICATIONS OF ISHS}, volume = {1218}, unique-id = {31872767}, issn = {0567-7572}, abstract = {From 2008, kiwifruit production and the total cultivated area decreased considerably, due to the pandemic spread of the bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa). This bacterium is able to infect host plants via natural openings or wounds, such as those caused by sucking insects. These insects are also known to be able to transmit other bacterial pathogens in different species. The present work aimed to investigate the role of Metcalfa pruinosa Say, 1830, one of the most common sucking insects to affect kiwifruit vines, in plant-to-plant transmission of Psa. The percentage of contaminated insects collected from infected orchards was evaluated to confirm the ecological relevance of M. pruinosa in the spread of bacterial canker of kiwifruit. This study demonstrated the survival of Psa in the M. pruinosa digestive trait, and the insect’s ability to vector the pathogen to healthy plants. © International Society for Horticultural Science. All rights reserved.}, keywords = {PSA; HOST PLANTS; 1930; Bacterial canker of kiwifruit; Insect-mediated disease transmission; Metcalfa pruinosa Say; Microscope visualization}, year = {2018}, eissn = {2406-6168}, pages = {269-273} } @article{MTMT:27524973, title = {Effect of entomopathogenic nematode, Steinernema feltiae, on survival and plasma phenoloxidase activity of Helicoverpa armigera (Hb) (Lepidoptera: Noctuidae) in laboratory conditions}, url = {https://m2.mtmt.hu/api/publication/27524973}, author = {Ebrahimi, Laleh and Shiri, Mohammadreza and Dunphy, Gary Brian}, doi = {10.1186/s41938-017-0016-x}, journal-iso = {EGYPT J BIOL PEST CO}, journal = {EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL}, volume = {28}, unique-id = {27524973}, issn = {1110-1768}, year = {2018}, eissn = {2536-9342} } @article{MTMT:30512081, title = {Effects of multiple sources of genetic drift on pathogen variation within hosts}, url = {https://m2.mtmt.hu/api/publication/30512081}, author = {Kennedy, David A. and Dwyer, Greg}, doi = {10.1371/journal.pbio.2004444}, journal-iso = {PLOS BIOL}, journal = {PLOS BIOLOGY}, volume = {16}, unique-id = {30512081}, issn = {1544-9173}, abstract = {Changes in pathogen genetic variation within hosts alter the severity and spread of infectious diseases, with important implications for clinical disease and public health. Genetic drift may play a strong role in shaping pathogen variation, but analyses of drift in pathogens have oversimplified pathogen population dynamics, either by considering dynamics only at a single scale-such as within hosts or between hosts-or by making drastic simplifying assumptions, for example, that host immune systems can be ignored or that transmission bottlenecks are complete. Moreover, previous studies have used genetic data to infer the strength of genetic drift, whereas we test whether the genetic drift imposed by pathogen population processes can be used to explain genetic data. We first constructed and parameterized a mathematical model of gypsy moth baculovirus dynamics that allows genetic drift to act within and between hosts. We then quantified the genome-wide diversity of baculovirus populations within each of 143 field-collected gypsy moth larvae using Illumina sequencing. Finally, we determined whether the genetic drift imposed by host-pathogen population dynamics in our model explains the levels of pathogen diversity in our data. We found that when the model allows drift to act at multiple scales-including within hosts, between hosts, and between years-it can accurately reproduce the data, but when the effects of drift are simplified by neglecting transmission bottlenecks and stochastic variation in virus replication within hosts, the model fails. A de novo mutation model and a purifying selection model similarly fail to explain the data. Our results show that genetic drift can play a strong role in determining pathogen variation and that mathematical models that account for pathogen population growth at multiple scales of biological organization can be used to explain this variation.}, year = {2018}, eissn = {1545-7885} } @article{MTMT:30512083, title = {Variation among populations in the immune protein composition of mother's milk reflects subsistence pattern}, url = {https://m2.mtmt.hu/api/publication/30512083}, author = {Klein, Laura D. and Huang, Jincui and Quinn, Elizabeth A. and Martin, Melanie A. and Breakey, Alicia A. and Gurven, Michael and Kaplan, Hillard and Valeggia, Claudia and Jasienska, Grazyna and Scelza, Brooke and Lebrilla, Carlito B. and Hinde, Katie}, doi = {10.1093/emph/eoy031}, journal-iso = {EVOL MED PUBLIC HLTH}, journal = {EVOLUTION MEDICINE AND PUBLIC HEALTH}, unique-id = {30512083}, abstract = {Lay Summary: Adaptive immune proteins in mothers' milk are more variable than innate immune proteins across populations and subsistence strategies. These results suggest that the immune defenses in milk are shaped by a mother's environment throughout her life.}, keywords = {human milk; milk immunofactors; maternal ecology; SIgA, lactoferin}, year = {2018}, eissn = {2050-6201}, pages = {230-245} } @article{MTMT:27524971, title = {DNA extracellular traps are part of the immune repertoire of Periplaneta americana}, url = {https://m2.mtmt.hu/api/publication/27524971}, author = {Nascimento, M T C and Silva, K P and Garcia, M C F and Medeiros, M N and Machado, E A and Nascimento, S B and Saraiva, E M}, doi = {10.1016/j.dci.2018.01.012}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {84}, unique-id = {27524971}, issn = {0145-305X}, year = {2018}, eissn = {1879-0089}, pages = {62-70} } @article{MTMT:27524974, title = {Morphological characterization of hemocyte types in some species belonging to Tettigoniidae and Pamphagidae (Insecta: Orthoptera)}, url = {https://m2.mtmt.hu/api/publication/27524974}, author = {Ozturk, Gurhan and Cakici, Ozlem and Arikan, Huseyin}, doi = {10.3906/zoo-1711-11}, journal-iso = {TURK J ZOOL}, journal = {TURKISH JOURNAL OF ZOOLOGY}, volume = {42}, unique-id = {27524974}, issn = {1300-0179}, year = {2018}, eissn = {1303-6114}, pages = {340-345} } @article{MTMT:27524972, title = {Interaction of entomopathogenic fungi with the host immune system}, url = {https://m2.mtmt.hu/api/publication/27524972}, author = {Qu, Shuang and Wang, Sibao}, doi = {10.1016/j.dci.2018.01.010}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {83}, unique-id = {27524972}, issn = {0145-305X}, year = {2018}, eissn = {1879-0089}, pages = {96-103} } @article{MTMT:30512088, title = {Innate humoral immune defences in mammals and insects: The same, with differences ?}, url = {https://m2.mtmt.hu/api/publication/30512088}, author = {Sheehan, Gerard and Garvey, Amy and Croke, Michael and Kavanagh, Kevin}, doi = {10.1080/21505594.2018.1526531}, journal-iso = {VIRULENCE}, journal = {VIRULENCE}, volume = {9}, unique-id = {30512088}, issn = {2150-5594}, abstract = {The insect immune response demonstrates many similarities to the innate immune response of mammals and a wide range of insects is now employed to assess the virulence of pathogens and produce results comparable to those obtained using mammals. Many of the humoral responses in insects and mammals are similar (e.g. insect transglutaminases and human clotting factor XIIIa) however a number show distinct differences. For example in mammals, melanization plays a role in protection from solar radiation and in skin and hair pigmentation. In contrast, insect melanization acts as a defence mechanism in which the proPO system is activated upon pathogen invasion. Human and insect antimicrobial peptides share distinct structural and functional similarities, insects produce the majority of their AMPs from the fat body while mammals rely on production locally at the site of infection by epithelial/mucosal cells. Understanding the structure and function of the insect immune system and the similarities with the innate immune response of mammals will increase the attractiveness of using insects as in vivo models for studying host - pathogen interactions.}, keywords = {COAGULATION; TOLL; INSECT; mammal; humoral immunity; Antimicrobial peptide; melanization}, year = {2018}, eissn = {2150-5608}, pages = {1625-1639} } @article{MTMT:31872763, title = {Animal Models for Melioidosis}, url = {https://m2.mtmt.hu/api/publication/31872763}, author = {Amemiya, K. and Bozue, J.A. and Cote, C.K. and Deshazer, D. and Soffler, C. and Welkos, S.L. and Worsham, P.L.}, doi = {10.1007/s40475-017-0131-5}, journal-iso = {Curr Trop Med Rep}, journal = {Current Tropical Medicine Reports}, volume = {4}, unique-id = {31872763}, abstract = {Purpose of Review: Development, testing, and evaluation of medical countermeasures for melioidosis are hampered by a lack of well-characterized and standardized animal models. Recent work has both refined existing animal models for this disease and identified new ones. Recent Findings: Head-to-head comparisons of mouse strains with varying susceptibility to the organism and using different routes of infection highlighted and confirmed important similarities and differences between murine models and exposure routes. Diabetic mouse models provided insight into the disease process in humans having this major risk factor. Large animal models, both livestock and non-human primate, have been established. Alternative (non-mammalian) models have been useful in identification of virulence factors and screening of therapeutic candidates. They hold potential for large-scale screening that would not be appropriate or practical for mammalian species. Summary: Recent advances in animal and alternative modeling will enhance our understanding of the organism and the disease process, as well as accelerating the development of medical countermeasures. © 2017, US Government (outside the USA).}, keywords = {review; human; aerosol; Virulence; risk factor; priority journal; innate immunity; nonhuman; nematode; latent period; animal models; Screening; in vitro models; hemagglutination; melioidosis; melioidosis; Burkholderia pseudomallei; Galleria mellonella; organ distribution; Medical countermeasures; amebic infection}, year = {2017}, eissn = {2196-3045}, pages = {208-222} } @article{MTMT:26891847, title = {Differential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniae}, url = {https://m2.mtmt.hu/api/publication/26891847}, author = {Arteaga, Blanco Luis Andres and Crispim, Josicelli Souza and Fernandes, Kenner Morais and de Oliveira, Leandro Licursi and Pereira, Monalessa Fabia and Soares, Bazzolli Denise Mara and Martins, Gustavo Ferreira}, doi = {10.1007/s00441-017-2653-5}, journal-iso = {CELL TISSUE RES}, journal = {CELL AND TISSUE RESEARCH}, volume = {370}, unique-id = {26891847}, issn = {0302-766X}, year = {2017}, eissn = {1432-0878}, pages = {153-168} } @article{MTMT:26674007, title = {Memory and specificity in the insect immune system: Current perspectives and future challenges}, url = {https://m2.mtmt.hu/api/publication/26674007}, author = {Cooper, D and Eleftherianos, I}, doi = {10.3389/fimmu.2017.00539}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {8}, unique-id = {26674007}, issn = {1664-3224}, year = {2017}, eissn = {1664-3224} } @article{MTMT:27049969, title = {Role of Metcalfa pruinosa as a Vector for Pseudomonas syringae pv. actinidiae}, url = {https://m2.mtmt.hu/api/publication/27049969}, author = {Donati, Irene and Mauri, Sofia and Buriani, Giampaolo and Cellini, Antonio and Spinelli, Francesco}, doi = {10.5423/PPJ.OA.04.2017.0074}, journal-iso = {PLANT PATHOLOGY J}, journal = {PLANT PATHOLOGY JOURNAL (KOREA)}, volume = {33}, unique-id = {27049969}, issn = {1598-2254}, year = {2017}, eissn = {2093-9280}, pages = {554-560} } @article{MTMT:26674006, title = {Screening, expression, purification and functional characterization of novel antimicrobial peptide genes from hermetia illucens (L.)}, url = {https://m2.mtmt.hu/api/publication/26674006}, author = {Elhag, O and Zhou, D and Song, Q and Soomro, AA and Cai, M and Zheng, L and Yu, Z and Zhang, J}, doi = {10.1371/journal.pone.0169582}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {12}, unique-id = {26674006}, issn = {1932-6203}, year = {2017}, eissn = {1932-6203} } @article{MTMT:27049413, title = {Gene expression and molecular characterization of a novel C-type lectin, encapsulation promoting lectin (EPL), in the rice armyworm, Mythimna separata}, url = {https://m2.mtmt.hu/api/publication/27049413}, author = {Ishihara, Teruhito and Maruyama, Yuki and Furukawa, Seiichi}, doi = {10.1016/j.ibmb.2017.08.008}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {89}, unique-id = {27049413}, issn = {0965-1748}, year = {2017}, eissn = {1879-0240}, pages = {51-57} } @article{MTMT:26674008, title = {Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection}, url = {https://m2.mtmt.hu/api/publication/26674008}, author = {Rossoni, RD and Fuchs, BB and De Barros, PP and Dos Santos, Velloso M and Jorge, AOC and Junqueira, JC and Mylonakis, E}, doi = {10.1371/journal.pone.0173332}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {12}, unique-id = {26674008}, issn = {1932-6203}, year = {2017}, eissn = {1932-6203} } @article{MTMT:31872773, title = {Energetics and the immune system: Trade-offs associated with non-acute levels of CRP in adolescent Gambian girls}, url = {https://m2.mtmt.hu/api/publication/31872773}, author = {Shattuck-Heidorn, H. and Reiches, M.W. and Prentice, A.M. and Moore, S.E. and Ellison, P.T.}, doi = {10.1093/emph/eow034}, journal-iso = {EVOL MED PUBLIC HLTH}, journal = {EVOLUTION MEDICINE AND PUBLIC HEALTH}, volume = {2017}, unique-id = {31872773}, abstract = {Background and objectives: The human immune system is an ever-changing composition of innumerable cells and proteins, continually ready to respond to pathogens or insults. The cost of maintaining this state of immunological readiness is rarely considered. In this paper we aim to discern a cost to nonacute immune function by investigating how low levels of C-reactive protein (CRP) relate to other energetic demands and resources in adolescent Gambian girls. Methodology: Data from a longitudinal study of 66 adolescent girls was used to test hypotheses around investment in immune function. Non-acute (under 2 mg/L) CRP was used as an index of immune function. Predictor variables include linear height velocity, adiposity, leptin, and measures of energy balance. Results: Non-acute log CRP was positively associated with adiposity (β = 0.16, P<0.001, R2=0.17) and levels of the adipokine leptin (β = 1.17, P = 0.006, R2=0.09). CRP was also negatively associated with increased investment in growth, as measured by height velocity (β =-0.58, P<0.001, R2=0.13) and lean mass deposition β =-0.42, P = 0.005, R2=0.08). Relationships between adiposity and growth explained some, but not all, of this association. We do not find that CRP was related to energy balance. Conclusions and implications: These data support a hypothesis that investment in non-acute immune function is facultative, and sensitive to energetic resources and demands. We also find support for an adaptive association between the immune system and adipose tissue. © The Author(s) 2017.}, keywords = {Body Weight; Adult; Adolescent; Female; C-REACTIVE PROTEIN; GROWTH; ARTICLE; PREDICTION; human; controlled study; OBESITY; INSULIN; immune system; Body Height; body composition; leptin; energy balance; puberty; C peptide; immune response; C reactive protein; longitudinal study; Energy transfer; Skinfold Thickness; Adipose Tissue; Adipose Tissue; Immune function; Life history theory; Height velocity}, year = {2017}, eissn = {2050-6201}, pages = {27-38} } @article{MTMT:26674009, title = {Evolution and function of thioester-containing proteins and the complement system in the innate immune response}, url = {https://m2.mtmt.hu/api/publication/26674009}, author = {Shokal, U and Eleftherianos, I}, doi = {10.3389/fimmu.2017.00759}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {8}, unique-id = {26674009}, issn = {1664-3224}, year = {2017}, eissn = {1664-3224} } @article{MTMT:26674005, title = {Wood ant colouration as an ecological indicator for the level of disturbance in managed coniferous forests}, url = {https://m2.mtmt.hu/api/publication/26674005}, author = {Skaldina, O and Sorvari, J}, doi = {10.1016/j.ecolind.2016.08.039}, journal-iso = {ECOL INDIC}, journal = {ECOLOGICAL INDICATORS}, volume = {72}, unique-id = {26674005}, issn = {1470-160X}, year = {2017}, eissn = {1872-7034}, pages = {444-451} } @article{MTMT:26891848, title = {Temperature stress and insect immunity}, url = {https://m2.mtmt.hu/api/publication/26891848}, author = {Wojda, Iwona}, doi = {10.1016/j.jtherbio.2016.12.002}, journal-iso = {J THERM BIOL: ENVIRON EVOL MEDICINE}, journal = {JOURNAL OF THERMAL BIOLOGY: ENVIRONMENT EVOLUTION AND MEDICINE}, volume = {68}, unique-id = {26891848}, issn = {0306-4565}, year = {2017}, eissn = {1879-0992}, pages = {96-103} } @article{MTMT:27090699, title = {Immune responses in the haemolymph and antimicrobial peptide expression in the abdomen of Apis mellifera challenged with Spiroplasma melliferum CH-1}, url = {https://m2.mtmt.hu/api/publication/27090699}, author = {Yang, Donghang and Zha, Guodong and Li, Xia and Gao, Han and Yu, Hanshou}, doi = {10.1016/j.micpath.2017.10.006}, journal-iso = {MICROB PATHOGENESIS}, journal = {MICROBIAL PATHOGENESIS}, volume = {112}, unique-id = {27090699}, issn = {0882-4010}, year = {2017}, eissn = {1096-1208}, pages = {279-287} } @article{MTMT:26203256, title = {Entomopathogenic Fungi: New Insights into Host-Pathogen Interactions}, url = {https://m2.mtmt.hu/api/publication/26203256}, author = {Butt, T M and Coates, C J and Dubovskiy, I M and Ratcliffe, N A}, doi = {10.1016/bs.adgen.2016.01.006}, editor = {Lovett, B and StLeger, RJ}, journal-iso = {ADV GENET}, journal = {ADVANCES IN GENETICS}, volume = {94}, unique-id = {26203256}, issn = {0065-2660}, year = {2016}, pages = {307-364} } @article{MTMT:26203255, title = {Galleria mellonella as a model host for microbiological and toxin research}, url = {https://m2.mtmt.hu/api/publication/26203255}, author = {Champion, Olivia L and Wagley, Sariqa and Titball, Richard W}, doi = {10.1080/21505594.2016.1203486}, journal-iso = {VIRULENCE}, journal = {VIRULENCE}, volume = {7}, unique-id = {26203255}, issn = {2150-5594}, year = {2016}, eissn = {2150-5608}, pages = {840-845} } @article{MTMT:26015798, title = {Long-Term Prophylactic Antibiotic Treatment: Effects on Survival, Immunocompetence and Reproduction Success of Parasemia plantaginis (Lepidoptera: Erebidae)}, url = {https://m2.mtmt.hu/api/publication/26015798}, author = {Dickel, Franziska and Freitak, Dalial and Mappes, Johanna}, doi = {10.1093/jisesa/iew035}, journal-iso = {J INSECT SCI}, journal = {JOURNAL OF INSECT SCIENCE}, volume = {16}, unique-id = {26015798}, issn = {1536-2442}, year = {2016}, eissn = {1536-2442} } @article{MTMT:26003334, title = {An overview of antifungal peptides derived from insect}, url = {https://m2.mtmt.hu/api/publication/26003334}, author = {Faruck, Mohammad Omer and Yusof, Faridah and Chowdhury, Silvia}, doi = {10.1016/j.peptides.2015.06.001}, journal-iso = {PEPTIDES}, journal = {PEPTIDES}, volume = {80}, unique-id = {26003334}, issn = {0196-9781}, year = {2016}, eissn = {1873-5169}, pages = {80-88} } @article{MTMT:25770903, title = {Climate change, nutrition and immunity: Effects of elevated CO2 and temperature on the immune function of an insect herbivore}, url = {https://m2.mtmt.hu/api/publication/25770903}, author = {Gherlenda, Andrew N and Haigh, Anthony M and Moore, Ben D and Johnson, Scott N and Riegler, Markus}, doi = {10.1016/j.jinsphys.2015.12.002}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {85}, unique-id = {25770903}, issn = {0022-1910}, year = {2016}, eissn = {1879-1611}, pages = {57-64} } @article{MTMT:26015797, title = {Sickness Behavior in Honey Bees}, url = {https://m2.mtmt.hu/api/publication/26015797}, author = {Kazlauskas, Nadia and Klappenbach, Martin and Depino, Amaicha M and Locatelli, Fernando F}, doi = {10.3389/fphys.2016.00261}, journal-iso = {FRONT PHYSIOL}, journal = {FRONTIERS IN PHYSIOLOGY}, volume = {7}, unique-id = {26015797}, year = {2016}, eissn = {1664-042X} } @article{MTMT:25770901, title = {De novo transcriptome analysis of Thitarodes jiachaensis before and after infection by the caterpillar fungus, Ophiocordyceps sinensis}, url = {https://m2.mtmt.hu/api/publication/25770901}, author = {Li, Shaosong and Zhong, Xin and Kan, Xutian and Gu, Li and Sun, Hongxia and Zhang, Guren and Liu, Xin}, doi = {10.1016/j.gene.2016.01.007}, journal-iso = {GENE}, journal = {GENE}, volume = {580}, unique-id = {25770901}, issn = {0378-1119}, year = {2016}, eissn = {1879-0038}, pages = {96-103} } @article{MTMT:26015799, title = {Identification and evaluation of cultivable gut bacteria associated with peach fruit fly, Bactrocera zonata (Diptera: Tephritidae)}, url = {https://m2.mtmt.hu/api/publication/26015799}, author = {Naaz, Naiyar and Choudhary, Jaipal S and Prabhakar, Chandra S and Maurya, Moanaro Sudarshan}, doi = {10.1007/s12600-016-0518-1}, journal-iso = {PHYTOPARASITICA}, journal = {PHYTOPARASITICA}, volume = {44}, unique-id = {26015799}, issn = {0334-2123}, year = {2016}, eissn = {1876-7184}, pages = {165-176} } @article{MTMT:25770902, title = {Mechanisms of nodule-specific melanization in the hemocoel of the silkworm, Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/25770902}, author = {Shu, Min and Mang, Dingze and Fu, Gege Sun and Tanaka, Shiho and Endo, Haruka and Kikuta, Shingo and Sato, Ryoichi}, doi = {10.1016/j.ibmb.2015.12.005}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {70}, unique-id = {25770902}, issn = {0965-1748}, year = {2016}, eissn = {1879-0240}, pages = {10-23}, orcid-numbers = {Kikuta, Shingo/0000-0002-3925-576X} } @article{MTMT:26674010, title = {Perplexing metabolomes in fungal-insect trophic interactions: A Terra incognita of mycobiocontrol mechanisms}, url = {https://m2.mtmt.hu/api/publication/26674010}, author = {Singh, D and Son, SY and Lee, CH}, doi = {10.3389/fmicb.2016.01678}, journal-iso = {FRONT MICROBIOL}, journal = {FRONTIERS IN MICROBIOLOGY}, volume = {7}, unique-id = {26674010}, issn = {1664-302X}, year = {2016}, eissn = {1664-302X} } @article{MTMT:26674011, title = {Conifer Monoterpene Chemistry during an Outbreak Enhances Consumption and Immune Response of an Eruptive Folivore}, url = {https://m2.mtmt.hu/api/publication/26674011}, author = {Trowbridge, AM and Bowers, MD and Monson, RK}, doi = {10.1007/s10886-016-0797-5}, journal-iso = {J CHEM ECOL}, journal = {JOURNAL OF CHEMICAL ECOLOGY}, volume = {42}, unique-id = {26674011}, issn = {0098-0331}, year = {2016}, eissn = {1573-1561}, pages = {1281-1292} } @article{MTMT:25801794, title = {Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing}, url = {https://m2.mtmt.hu/api/publication/25801794}, author = {Tsai, CJ-Y and Loh, JMS and Proft, T}, doi = {10.1080/21505594.2015.1135289}, journal-iso = {VIRULENCE}, journal = {VIRULENCE}, volume = {7}, unique-id = {25801794}, issn = {2150-5594}, year = {2016}, eissn = {2150-5608}, pages = {214-229} } @{MTMT:31872774, title = {Interactions between parasitic ciliates and their hosts: Ichthyophthirius multifiliis and cryptocaryon irritans as examples}, url = {https://m2.mtmt.hu/api/publication/31872774}, author = {Zaila, K.E. and Cho, D. and Chang, W.-J.}, booktitle = {Biocommunication of Ciliates}, doi = {10.1007/978-3-319-32211-7_18}, unique-id = {31872774}, abstract = {Among all known parasitic ciliates, Ichthyophthirius multifiliis and Cryptocaryon irritans are probably the two best studied "model parasites," which cause the white spot diseases in freshwater and marine fish, respectively. These two parasites are not phylogenetically closely related, but share very similar life cycle and pathological manifestations. Both parasites invade and feed in the epithelial layer of the skin and gill, and trigger both innate and adaptive immune responses in fish hosts, locally and systemically. The parasite-host interactions not only help add to our knowledge of fish immunity, but also shed light on research regarding other less well-studied ciliate parasites. Future research directions are also discussed in this chapter. © Springer International Publishing Switzerland 2016. All rights reserved.}, year = {2016}, pages = {327-350} } @article{MTMT:25283690, title = {Importance of adhesins in virulence of Paracoccidioides spp.}, url = {https://m2.mtmt.hu/api/publication/25283690}, author = {de Oliveira, Haroldo C and da Silva, Julhiany de Fatima and Scorzoni, Liliana and Marcos, Caroline M and Rossi, Sueen A and de Paula, e Silva Ana C A and Assato, Patricia A and da Silva, Rosangela A M and Fusco-Almeida, Ana M and Mendes-Giannini, Maria J S}, doi = {10.3389/fmicb.7015.00303}, journal-iso = {FRONT MICROBIOL}, journal = {FRONTIERS IN MICROBIOLOGY}, volume = {6}, unique-id = {25283690}, issn = {1664-302X}, year = {2015}, eissn = {1664-302X} } @article{MTMT:24983669, title = {Importance of adhesins in virulence of Paracoccidioides spp.}, url = {https://m2.mtmt.hu/api/publication/24983669}, author = {de Oliveira, HC and da Silva, JF and Scorzoni, L and Marcos, CM and Rossi, SA and de Paula, e Silva ACA and Assato, PA and da Silva, RAM and Fusco-Almeida, AM and Mendes-Giannini, MJS}, doi = {10.3389/fmicb.2015.00303}, journal-iso = {FRONT MICROBIOL}, journal = {FRONTIERS IN MICROBIOLOGY}, volume = {6}, unique-id = {24983669}, issn = {1664-302X}, year = {2015}, eissn = {1664-302X} } @article{MTMT:24728915, title = {Tweedle cuticular protein BmCPT1 is involved in innate immunity by participating in recognition of Escherichia coli}, url = {https://m2.mtmt.hu/api/publication/24728915}, author = {Liang, JB and Wang, T and Xiang, ZH and He, NJ}, doi = {10.1016/j.ibmb.2014.11.004}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {58}, unique-id = {24728915}, issn = {0965-1748}, year = {2015}, eissn = {1879-0240}, pages = {76-88} } @article{MTMT:25283689, title = {Transcriptomic insight into the immune defenses in the ghost moth, Hepialus xiaojinensis, during an Ophiocordyceps sinensis fungal infection}, url = {https://m2.mtmt.hu/api/publication/25283689}, author = {Meng, Qian and Yu, Hai-Ying and Zhang, Huan and Zhu, Wei and Wang, Meng-Long and Zhang, Ji-Hong and Zhou, Gui-Ling and Li, Xuan and Qin, Qi-Lian and Hu, Song-Nian and Zou, Zhen}, doi = {10.1016/j.ibmb.2015.06.014}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {64}, unique-id = {25283689}, issn = {0965-1748}, year = {2015}, eissn = {1879-0240}, pages = {1-15} } @article{MTMT:25801796, title = {Bacterial infection increases reproductive investment in burying beetles}, url = {https://m2.mtmt.hu/api/publication/25801796}, author = {Reavey, CE and Silva, FWS and Cotter, SC}, doi = {10.3390/insects6040926}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {6}, unique-id = {25801796}, year = {2015}, eissn = {2075-4450}, pages = {926-942} } @article{MTMT:24983667, title = {The specificity of immune priming in silkworm, Bombyx mori, is mediated by the phagocytic ability of granular cells}, url = {https://m2.mtmt.hu/api/publication/24983667}, author = {Wu, G and Li, M and Liu, Y and Ding, Y and Yi, Y}, doi = {10.1016/j.jinsphys.2015.07.004}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {81}, unique-id = {24983667}, issn = {0022-1910}, year = {2015}, eissn = {1879-1611}, pages = {60-68} } @article{MTMT:24983671, title = {Early responses of silkworm midgut to microsporidium infection - A Digital Gene Expression analysis}, url = {https://m2.mtmt.hu/api/publication/24983671}, author = {Yue, Y-J and Tang, X-D and Xu, L and Yan, W and Li, Q-L and Xiao, S-Y and Fu, X-L and Wang, W and Li, N and Shen, Z-Y}, doi = {10.1016/j.jip.2014.10.003}, journal-iso = {J INVERTEBR PATHOL}, journal = {JOURNAL OF INVERTEBRATE PATHOLOGY}, volume = {124}, unique-id = {24983671}, issn = {0022-2011}, year = {2015}, eissn = {1096-0805}, pages = {6-14} } @article{MTMT:24094417, title = {Fat body, fat pad and adipose tissues in invertebrates and vertebrates: the nexus}, url = {https://m2.mtmt.hu/api/publication/24094417}, author = {Azeez, OI and Meintjes, R and Chamunorwa, JP}, doi = {10.1186/1476-511X-13-71}, journal-iso = {LIPIDS HEALTH DIS}, journal = {LIPIDS IN HEALTH AND DISEASE}, volume = {13}, unique-id = {24094417}, issn = {1476-511X}, year = {2014}, eissn = {1476-511X} } @book{MTMT:27617323, title = {Rare Diseases and Orphan Drugs: Keys to Understanding and Treating the Common Diseases}, url = {https://m2.mtmt.hu/api/publication/27617323}, isbn = {9780124200098}, author = {Berman, JJ}, doi = {10.1016/C2013-0-09838-6}, unique-id = {27617323}, year = {2014} } @article{MTMT:24983673, title = {β-Phosphoglucomutase contributes to aciduricity in streptococcus mutans}, url = {https://m2.mtmt.hu/api/publication/24983673}, author = {Buckley, AA and Faustoferri, RC and Quivey, Jr RG}, doi = {10.1099/mic.0.075754-0}, journal-iso = {MICROBIOL-SGM}, journal = {MICROBIOLOGY-SGM}, volume = {160}, unique-id = {24983673}, issn = {1350-0872}, year = {2014}, eissn = {1465-2080}, pages = {818-827} } @article{MTMT:24313037, title = {Effect of an entomopathogenic nematode, Steinernema carpocapsae on haemocyte profile and phenoloxidase activity of the Colorado potato beetle, Leptinotarsa decemlineata}, url = {https://m2.mtmt.hu/api/publication/24313037}, author = {Ebrahimi, L and Niknam, G and Dunphy, GB and Toorchi, M}, doi = {10.1080/09583157.2014.942595}, journal-iso = {BIOCONTROL SCI TECHN}, journal = {BIOCONTROL SCIENCE AND TECHNOLOGY}, volume = {24}, unique-id = {24313037}, issn = {0958-3157}, year = {2014}, eissn = {1360-0478}, pages = {1383-1393} } @article{MTMT:24313035, title = {Pathogen Growth in Insect Hosts: Inferring the Importance of Different Mechanisms Using Stochastic Models and Response-Time Data}, url = {https://m2.mtmt.hu/api/publication/24313035}, author = {Kennedy, DA and Dukic, V and Dwyer, G}, doi = {10.1086/677308}, journal-iso = {AM NAT}, journal = {AMERICAN NATURALIST}, volume = {184}, unique-id = {24313035}, issn = {0003-0147}, year = {2014}, eissn = {1537-5323}, pages = {407-423} } @article{MTMT:25122863, title = {Plasticity of nervous and immune systems in different species: The role of proteasomes}, url = {https://m2.mtmt.hu/api/publication/25122863}, author = {Lyupina, YV and Orlova, AS and Gornostayev, NG and Karpova, YD and Mikhailov, VS and Sharova, NP}, journal-iso = {ZH OBSHCH BIOL}, journal = {ZHURNAL OBSHCHEI BIOLOGII}, volume = {75}, unique-id = {25122863}, issn = {0044-4596}, year = {2014}, pages = {3-24} } @article{MTMT:24983677, title = {Honey bee hemocyte profiling by flow cytometr}, url = {https://m2.mtmt.hu/api/publication/24983677}, author = {Marringa, WJ and Krueger, MJ and Burritt, NL and Burritt, JB}, doi = {10.1371/journal.pone.0108486}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {9}, unique-id = {24983677}, issn = {1932-6203}, year = {2014}, eissn = {1932-6203} } @article{MTMT:24983679, title = {Atmospheric change and induced plant secondary metabolites - Are we reshaping the building blocks of multi-trophic interactions?}, url = {https://m2.mtmt.hu/api/publication/24983679}, author = {Ode, PJ and Johnson, SN and Moore, BD}, doi = {10.1016/j.cois.2014.09.006}, journal-iso = {CURR OPIN INSECT SCI}, journal = {CURRENT OPINION IN INSECT SCIENCE}, volume = {5}, unique-id = {24983679}, issn = {2214-5745}, year = {2014}, eissn = {2214-5753}, pages = {57-65} } @article{MTMT:24313036, title = {Effects of Interaction between Temperature Conditions and Copper Exposure on Immune Defense and Other Life-History Traits of the Blow Fly Protophormia terraenovae}, url = {https://m2.mtmt.hu/api/publication/24313036}, author = {Polkki, M and Kangassalo, K and Rantala, MJ}, doi = {10.1021/es501880b}, journal-iso = {ENVIRON SCI TECHNOL}, journal = {ENVIRONMENTAL SCIENCE & TECHNOLOGY}, volume = {48}, unique-id = {24313036}, issn = {0013-936X}, year = {2014}, eissn = {1520-5851}, pages = {8793-8799} } @article{MTMT:24863914, title = {Immunity-related genes in Ixodes scapularis-perspectives from genorne information}, url = {https://m2.mtmt.hu/api/publication/24863914}, author = {Smith, Alexis A and Pal, Utpal}, doi = {10.3389/fcimb.2014.00116}, journal-iso = {FRONT CELL INFECT MI}, journal = {FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY}, volume = {4}, unique-id = {24863914}, issn = {2235-2988}, year = {2014}, eissn = {2235-2988} } @article{MTMT:24094418, title = {Factors functioning in nodule melanization of insects and their mechanisms of accumulation in nodules}, url = {https://m2.mtmt.hu/api/publication/24094418}, author = {Tokura, A and Fu, GS and Sakamoto, M and Endo, H and Tanaka, S and Kikuta, S and Tabunoki, H and Sato, R}, doi = {10.1016/j.jinsphys.2013.11.003}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {60}, unique-id = {24094418}, issn = {0022-1910}, year = {2014}, eissn = {1879-1611}, pages = {40-49} } @article{MTMT:23355737, title = {Bacillus thuringiensis Induces Cellular Stress in the Mosquito Vector, Culex pipiens, Prior to Death}, url = {https://m2.mtmt.hu/api/publication/23355737}, author = {Ahmed, AM}, journal-iso = {PAK J ZOOL}, journal = {PAKISTAN JOURNAL OF ZOOLOGY}, volume = {45}, unique-id = {23355737}, issn = {0030-9923}, year = {2013}, eissn = {0030-9923}, pages = {129-139} } @article{MTMT:24094420, title = {Immunohistochemical analysis of the role of hemocytin in nodule formation in the larvae of the silkworm, Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/24094420}, author = {Arai, I and Ohta, M and Suzuki, A and Tanaka, S and Yoshizawa, Y and Sato, R}, journal-iso = {J INSECT SCI}, journal = {JOURNAL OF INSECT SCIENCE}, volume = {13}, unique-id = {24094420}, issn = {1536-2442}, year = {2013}, eissn = {1536-2442} } @article{MTMT:23469566, title = {An analysis of the structural and functional similarities of insect hemocytes and mammalian phagocytes}, url = {https://m2.mtmt.hu/api/publication/23469566}, author = {Browne, N and Heelan, M and Kavanagh, K}, doi = {10.4161/viru.25906}, journal-iso = {VIRULENCE}, journal = {VIRULENCE}, volume = {4}, unique-id = {23469566}, issn = {2150-5594}, year = {2013}, eissn = {2150-5608}, pages = {597-603} } @article{MTMT:31872768, title = {Evaluation of Innate Immune Stimulating Activity of Polysaccharides Using a Silkworm (Bombyx Mori) Muscle Contraction Assay}, url = {https://m2.mtmt.hu/api/publication/31872768}, author = {Fujiyuki, T. and Takeda, T. and Hamamoto, H. and Ishii, K. and Urai, M. and Kataoka, K. and Shibata, S. and Sekimizu, K.}, doi = {10.5582/ddt.2012.v6.2.88}, journal-iso = {DRUG DISCOV THER}, journal = {DRUG DISCOVERIES & THERAPEUTICS}, volume = {6}, unique-id = {31872768}, issn = {1881-7831}, abstract = {In silkworm larvae, the mature form of paralytic peptide (PP), an insect cytokine, is produced from pro-PP in association with activation of innate immune responses, resulting in slow muscle contraction. We utilized this reaction, muscle contraction in silkworms coupled with innate immunity stimulation, to quantitatively measure the innate immune stimulating activity of various natural polysaccharides. β-Glucan of Gyrophora esculenta (GE-3), fucoidan from sporophyll of Undaria pinnatifida, and curldan induced silkworm muscle contraction. We further demonstrated that GE-3 had therapeutic effects on silkworms infected by baculovirus. Based on these findings, we propose that the silkworm muscle contraction assay is useful for screening substances that stimulate innate immunity before evaluating therapeutic effectiveness in mammals. © 2012, International Research and Cooperation Association for Bio & Socio-Sciences Advancement. All rights reserved.}, keywords = {innate immunity; polysaccharide; Silkworm}, year = {2013}, pages = {88-93} } @article{MTMT:23355891, title = {A comprehensive analysis of the Manduca sexta immunotranscriptome}, url = {https://m2.mtmt.hu/api/publication/23355891}, author = {Gunaratna, RT and Jiang, HB}, doi = {10.1016/j.dci.2012.10.004}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {39}, unique-id = {23355891}, issn = {0145-305X}, year = {2013}, eissn = {1879-0089}, pages = {388-398} } @article{MTMT:23355736, title = {Gibbsiella papilionis sp nova, isolated from the intestinal tract of the butterfly Mycalesis gotama, and emended description of the genus Gibbsiella}, url = {https://m2.mtmt.hu/api/publication/23355736}, author = {Kim, PS and Shin, NR and Kim, JY and Yun, JH and Hyun, DW and Bae, JW}, doi = {10.1099/ijs.0.045880-0}, journal-iso = {INT J SYST EVOL MICR}, journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}, volume = {63}, unique-id = {23355736}, issn = {1466-5026}, year = {2013}, eissn = {1466-5034}, pages = {2607-2611} } @book{MTMT:27009925, title = {Physiological Systems in Insects: Third Edition}, url = {https://m2.mtmt.hu/api/publication/27009925}, isbn = {9780124158191}, author = {Klowden, MJ}, doi = {10.1016/C2011-0-04120-0}, publisher = {Elsevier Inc.}, unique-id = {27009925}, year = {2013} } @article{MTMT:23466769, title = {Synchrotron radiation analysis in the study of pollution in the ring-billed gull (larus delawarensis): a novel application of the technique}, url = {https://m2.mtmt.hu/api/publication/23466769}, author = {Martin, RR and Naftel, SJ and Doucet, SM and Hanley, D and Weisner, CG}, doi = {10.1002/xrs.2500}, journal-iso = {X-RAY SPECTROM}, journal = {X-RAY SPECTROMETRY}, volume = {42}, unique-id = {23466769}, issn = {0049-8246}, year = {2013}, eissn = {1097-4539}, pages = {437-441} } @article{MTMT:23355732, title = {The non-mammalian host Galleria mellonella can be used to study the virulence of the fungal pathogen Candida tropicalis and the efficacy of antifungal drugs during infection by this pathogenic yeast}, url = {https://m2.mtmt.hu/api/publication/23355732}, author = {Mesa-Arango, AC and Forastiero, A and Bernal-Martinez, L and Cuenca-Estrella, M and Mellado, E and Zaragoza, O}, doi = {10.3109/13693786.2012.737031}, journal-iso = {MED MYCOL}, journal = {MEDICAL MYCOLOGY}, volume = {51}, unique-id = {23355732}, issn = {1369-3786}, year = {2013}, eissn = {1460-2709}, pages = {461-472} } @article{MTMT:23469565, title = {Weissella diestrammenae sp. nov., isolated from the gut of a camel cricket (Diestrammena coreana)}, url = {https://m2.mtmt.hu/api/publication/23469565}, author = {Oh, SJ and Shin, N-R and Hyun, D-W and Kim, PS and Kim, JY and Kim, M-S and Yun, J-H and Bae, J-W}, doi = {10.1099/ijs.0.047548-0}, journal-iso = {INT J SYST EVOL MICR}, journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}, volume = {63}, unique-id = {23469565}, issn = {1466-5026}, year = {2013}, eissn = {1466-5034}, pages = {2951-2956} }