@article{MTMT:33181149, title = {Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/33181149}, author = {Chen, Lijuan and Zhang, Zhongjie and Chen, Kai and Yu, Ye and Hu, Bo and Song, Hongsheng and Liu, Xiaojing}, doi = {10.3390/biology11091313}, journal-iso = {BIOLOGY-BASEL}, journal = {BIOLOGY-BASEL}, volume = {11}, unique-id = {33181149}, abstract = {Simple Summary Most insects species enter a diapause period to ensure their survival under unsuitable environments. In the silkworm, Bombyx mori, diapause occurs at the embryonic stage and is regulated by the diapause hormone (DH). Here, we directly injected DH into the female pupae and investigated the DH receptor expression pattern. Using RNA sequencing, we analyzed the gene expression profile in the developing ovaries after the injection of DH. Diapause is a form of dormancy that organisms use to adapt to extreme environments by exhibiting developmental arrest. In the silkworm, Bombyx mori, diapause is thought to be elicited by diapause hormone (DH) signaling, which consists of interactions between DH and the DH receptor (DHR). However, the steps downstream of the DH signaling pathway are largely unknown. In the present study, we directly injected synthesized DH into the female pupae of a multivoltine, non-diapausing strain at 36 h after pupation. We found that the mRNA level of DHR declined at 4 h and recovered at 12 h after the injection of DH. Thus, we sequenced the transcriptome of the ovaries at 4 h and 12 h after the injection of DH. We identified 60 and 221 differentially expressed genes at 4 h and 12 h after the injection, respectively. All DEGs were identified, relating to 20E-related genes, JH-related genes, cellular detoxification, ribosomal proteins, lipid metabolism, and epigenetic modifications. Eleven genes were selected from the above categories to verify the transcriptome data. The qRT-PCR and RNA-Seq expression patterns of the genes were consistent, which indicated the authenticity and reliability of the transcriptome data. This study dramatically expands upon our knowledge of gene expression variation at the early phase of DH release.}, keywords = {RNA sequencing; Silkworm; diapause; Diapause hormone}, year = {2022}, eissn = {2079-7737} } @article{MTMT:32970497, title = {Broad complex and wing development in cockroaches}, url = {https://m2.mtmt.hu/api/publication/32970497}, author = {Fernandez-Nicolas, Ana and Ventos-Alfonso, Alba and Kamsoi, Orathai and Clark-Hachtel, Courtney and Tomoyasu, Yoshinori and Belles, Xavier}, doi = {10.1016/j.ibmb.2022.103798}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {147}, unique-id = {32970497}, issn = {0965-1748}, abstract = {In hemimetabolan insects, the transcription factor Broad complex (Br-C) promotes wing growth and development during the nymphal period. We wondered whether Br-C could trigger the initiation of wing development, using the cockroach Blattella germanica as a model. We show that first instar nymphs have their unique identity of these three thoracic segments specified. During embryogenesis, the expression of Br-C and some wing-related genes show two matching waves. The first takes place before the formation of the germ band, which might be involved in the establishment of various developmental fields including a potential "wing field", and the second wave around organogenesis, possibly involved in the initiation of wing development. However, the expression of Br-C in early embryogenesis concentrates in the developing central nervous system, thus not co-localizing with the expression of the typical wing-related gene vestigial, which is expressed at the edge of the thoracic and abdominal segments. This suggests that Br-C is not specifically involved in the establishment of a potential "wing field" in early embryogenesis. Moreover, maternal RNAi for Br-C depletes the first wave of Br-C expression but does not affect the early expression of wing-related genes. As maternal Br-C RNAi did not deplete the second expression wave of Br-C, we could not evaluate if Br-C is involved in the initiation of wing development. Alternatively, using nymphal RNAi of Br-C and Sex combs reduced (Scr), we show that Br-C contributes to the formation of ectopic wing structures that develop in the prothorax when Scr is depleted. The gene most clearly influenced by Br-C RNAi is nubbin (nub), which, in nymphs is crucial for wing growth. Together, these results suggest that Br-C does not specifically contribute to the establishment of the "wing field", but it does seem important later, in the initiation of wing development, enhancing the expression of wing-related genes, especially nub. This supports the hypothesis previously proposed by the authors, whereby Br-C might have facilitated the evolution of holometaboly. However, there is no doubt that other factors have also contributed to this evolution.}, keywords = {nubbin; Broad Complex; Wing-related genes; Initiation of wing development; Blattella}, year = {2022}, eissn = {1879-0240}, orcid-numbers = {Belles, Xavier/0000-0002-1566-303X} } @article{MTMT:32970498, title = {Ecdysone exerts biphasic control of regenerative signaling, coordinating the completion of regeneration with developmental progression}, url = {https://m2.mtmt.hu/api/publication/32970498}, author = {Karanja, Faith and Sahu, Subhshri and Weintraub, Sara and Bhandari, Rajan and Jaszczak, Rebecca and Sitt, Jason and Halme, Adrian}, doi = {10.1073/pnas.2115017119}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {119}, unique-id = {32970498}, issn = {0027-8424}, abstract = {In Drosophila melanogaster, loss of regenerative capacity in wing imaginal discs coincides with an increase in systemic levels of the steroid hormone ecdysone, a key coordinator of their developmental progression. Regenerating discs release the relaxin hormone Dilp8 (Drosophila insulin-like peptide 8) to limit ecdysone synthesis and extend the regenerative period. Here, we describe how regenerating tissues produce a biphasic response to ecdysone levels: lower concentrations of ecdysone promote local and systemic regenerative signaling, whereas higher concentrations suppress regeneration through the expression of broad splice isoforms. Ecdysone also promotes the expression of wingless during both regeneration and normal development through a distinct regulatory pathway. This dual role for ecdysone explains how regeneration can still be completed successfully in dilp8(-)mutant larvae: higher ecdysone levels increase the regenerative activity of tissues, allowing regeneration to reach completion in a shorter time. From these observations, we propose that ecdysone hormone signaling functions to coordinate regeneration with developmental progression.}, keywords = {regeneration; ecdysone; BROAD; wingless; Dilp8}, year = {2022}, eissn = {1091-6490}, orcid-numbers = {Bhandari, Rajan/0000-0001-9336-955X; Jaszczak, Rebecca/0000-0003-2588-8790} } @article{MTMT:32934648, title = {Roles of Kruppel Homolog 1 and Broad-Complex in the Development of Dendroctonus armandi (Coleoptera: Scolytinae)}, url = {https://m2.mtmt.hu/api/publication/32934648}, author = {Sun, Ya-Ya and Fu, Dan-Yang and Liu, Bin and Wang, Lin-Jun and Chen, Hui}, doi = {10.3389/fphys.2022.865442}, journal-iso = {FRONT PHYSIOL}, journal = {FRONTIERS IN PHYSIOLOGY}, volume = {13}, unique-id = {32934648}, abstract = {In insects, metamorphosis is controlled by juvenile hormone (JH) and 20-hydroxyecdysone (20E). Kruppel homolog 1 (Kr-h1), a key JH-early inducible gene, is responsible for the suppression of metamorphosis and the regulation of the Broad-Complex (Br-C) gene, which is induced by 20E and functions as a "pupal specifier". In this study, we identified and characterized the expression patterns and tissue distribution of DaKr-h1 and DaBr-C at various developmental stages of Dendroctonus armandi. The expression of the two genes was induced by JH analog (JHA) methoprene and 20E, and their functions were investigated by RNA interference. DaKr-h1 and DaBr-C were predominantly expressed in the heads of larvae and were significantly downregulated during the molting stage. In contrast, the DaKr-h1 transcript level was highest in the adult anterior midgut. DaBr-C was mainly expressed in female adults, with the highest transcript levels in the ovaries. In the larval and pupal stages, both JHA and 20E significantly induced DaKr-h1, but only 20E significantly induced DaBr-C, indicating the importance of hormones in metamorphosis. DaKr-h1 knockdown in larvae upregulated DaBr-C expression, resulting in precocious metamorphosis from larvae to pupae and the formation of miniature pupae. DaKr-h1 knockdown in pupae suppressed DaBr-C expression, increased emergence, caused abnormal morphology, and caused the formation of small-winged adults. These results suggest that DaKr-h1 is required for the metamorphosis of D. armandi. Our findings provide insight into the roles of DaKr-h1 and DaBr-C in JH-induced transcriptional repression and highlight DaKr-h1 as a potential target for metamorphosis suppression in D. armandi.}, keywords = {RNA Interference; 20-HYDROXYECDYSONE; Juvenile hormone; Broad-Complex; Kruppel homolog 1; Dendroctonus armandi}, year = {2022}, eissn = {1664-042X} } @article{MTMT:32423816, title = {How stage identity is established in insects: the role of the Metamorphic Gene Network}, url = {https://m2.mtmt.hu/api/publication/32423816}, author = {Martin, David and Chafino, Silvia and Franch-Marro, Xavier}, doi = {10.1016/j.cois.2020.10.002}, journal-iso = {CURR OPIN INSECT SCI}, journal = {CURRENT OPINION IN INSECT SCIENCE}, volume = {43}, unique-id = {32423816}, issn = {2214-5745}, abstract = {Proper formation of adult insects requires the integration of spatial and temporal regulatory axes. Whereas spatial information confers identity to each tissue, organ and appendage, temporal information specifies at which stage of development the animal is. Regardless of the type of post embryonic development, either hemimetabolous or holometabolous, temporal specificity is achieved through interactions between the temporal identity genes Kr-h1, E93 and Br-C, whose sequential expression is controlled by the two major developmental hormones, 20-hydroxyecdysone and Juvenile hormone. Given the intimate regulatory connection between these three factors to specify life stage identity, we dubbed the regulatory axis that comprises these genes as the Metamorphic Gene Network (MGN). In this review, we survey the molecular mechanisms underlying the control by the MGN of stage identity and progression in hemimetabolous and holometabolous insects.}, year = {2021}, eissn = {2214-5753}, pages = {29-38} } @article{MTMT:32312190, title = {From genes to shape during metamorphosis: a history}, url = {https://m2.mtmt.hu/api/publication/32312190}, author = {Thompson, Barry J.}, doi = {10.1016/j.cois.2020.08.008}, journal-iso = {CURR OPIN INSECT SCI}, journal = {CURRENT OPINION IN INSECT SCIENCE}, volume = {43}, unique-id = {32312190}, issn = {2214-5745}, abstract = {Metamorphosis (Greek for a state of transcending-form or change-in-shape) refers to a dramatic transformation of an animal's body structure that occurs after development of the embryo or larva in many species. The development of a fly (or butterfly) from a crawling larva (or caterpillar) that forms a pupa (or chrysalis) before eclosing as a flying adult is a classic example of metamorphosis that captures the imagination and has been immortalized in children's books. Powerful genetic experiments in the fruit fly Drosophila melanogaster have revealed how genes can instruct the behaviour of individual cells to control patterns of tissue growth, mechanical force, cell-cell adhesion and cell matrix adhesion drive morphogenetic change in epithelial tissues. Together, the distribution of mass, force and resistance determines cell shape changes, cell-cell rearrangements, and/or the orientation of cell divisions to generate the final form of the tissue. In organising tissue shape, genes harness the power of self-organisation to determine the collective behaviour of molecules and cells, which can often be reproduced in computer simulations of cell polarity and/or tissue mechanics. This review highlights fundamental discoveries in epithelial morphogenesis made by pioneers who were fascinated by metamorphosis, including D'Arcy Thompson, Conrad Waddington, Dianne Fristrom and Antonio Garcia-Bellido.}, year = {2021}, eissn = {2214-5753}, pages = {1-10} } @article{MTMT:31685453, title = {Transcriptional regulation of cuticular genes during insect metamorphosis}, url = {https://m2.mtmt.hu/api/publication/31685453}, author = {Ali, Md Saheb and Takaki, Keiko}, doi = {10.2741/4796}, journal-iso = {FRONT BIOSCI-LANDMARK}, journal = {FRONTIERS IN BIOSCIENCE-LANDMARK}, volume = {25}, unique-id = {31685453}, issn = {2768-6701}, abstract = {In some adult holometabolous insects, specific epithelial tissues form imaginal discs, small sac-like clusters of cells that form in the larval body. During metamorphosis, in response to hormones, the discs undergo dramatic changes including cell proliferation and differentiation. In Bombyx mori insects, the cuticular protein (CP) genes, which are expressed at prepupal stage in the wing discs, are divided into six groups according to their developmental expression profile and responsiveness to steroid hormones. In this article, we discuss the expression of CP genes in the wing disc, and examine the molecular mechanisms by which metamorphosis and cell transformations are regulated by hormones in insects.}, keywords = {review; ecdysone; imaginal disc; Bombyx mori; Cuticle proteins}, year = {2020}, eissn = {2768-6698}, pages = {106-117} } @article{MTMT:31466713, title = {Silencing Br-C impairs larval development and chitin synthesis in Lymantria dispar larvae}, url = {https://m2.mtmt.hu/api/publication/31466713}, author = {Ding, Nan and Wang, Zhiying and Geng, Nannan and Zou, Hang and Zhang, Guocai and Cao, Chuanwang and Li, Xingpeng and Zou, Chuanshan}, doi = {10.1016/j.jinsphys.2020.104041}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {122}, unique-id = {31466713}, issn = {0022-1910}, abstract = {In insects, 20-hydroxyecdysone (20E) mediates developmental transitions and regulates molting processes through activation of a series of transcription factors. Broad-Complex (Br-C), a vital gene in the 20E signalling pathway, plays crucial roles during insect growth processes. However, whether Br-C affects chitin synthesis in insects remains unclear. In the present study, the Br-C gene from Lymantria dispar, a notorious defoliator of forestry, was identified based on transcriptome data, and subjected to bioinformatic analysis. The regulatory functions of LdBr-C in chitin synthesis and metabolism in L. dispar larvae were analysed by RNA interference (RNAi). The full-length LdBr-C gene (1431 bp) encodes a 477 amino acid (aa) polypeptide containing a common BRcore region (391 aa) at the N-terminus and a C-terminal Zinc finger domain (56 aa) harbouring two characteristic C2H2 motifs (CXXC and HXXXXH). Phylogenetic analyses showed that LdBr-C shares highest homology and identity with Br-C isoform 7 (83.12%) of Helicoverpa armigera. Expression profiles indicate that LdBr-C was expressed throughout larval and pupal stages, and highly expressed in prepupal and pupal stages. Furthermore, LdBr-C expression was strongly induced by exogenous 20E, and suppressed dramatically after application of dsLdBr-C. Bioassay results showed that knockdown of LdBr-C caused larval developmental deformity, significant weight loss, and a mortality rate of 67.18%. Knockdown of LdBr-C significantly down-regulated transcription levels of eight critical genes (LdTre1, LdTre2, LdG6PI, LdUAP, LdCHS1, LdCHS2, LdTPS and LdCHT) related to chitin synthesis and metabolism, thereby lowering the chitin content in the midgut and epidermis. Our findings demonstrate that Br-C knockdown impairs larval development and chitin synthesis in L. dispar.}, keywords = {Chitin synthesis; Lymantria dispar; Broad Complex; 20E signalling pathway; RANi}, year = {2020}, eissn = {1879-1611} } @article{MTMT:31466711, title = {let-7-Complex MicroRNAs Regulate Broad-Z3, Which Together with Chinmo Maintains Adult Lineage Neurons in an Immature State}, url = {https://m2.mtmt.hu/api/publication/31466711}, author = {Wu, Yen-Chi and Chawla, Geetanjali and Sokol, Nicholas}, doi = {10.1534/g3.120.401042}, journal-iso = {G3-GENES GENOM GENET}, journal = {G3-GENES GENOMES GENETICS}, volume = {10}, unique-id = {31466711}, issn = {2160-1836}, abstract = {During Drosophila melanogaster metamorphosis, arrested immature neurons born during larval development differentiate into their functional adult form. This differentiation coincides with the downregulation of two zinc-finger transcription factors, Chronologically Inappropriate Morphogenesis (Chinmo) and the Z3 isoform of Broad (Br-Z3). Here, we show that br-Z3 is regulated by two microRNAs, let-7 and miR-125, that are activated at the larval-to-pupal transition and are known to also regulate . The br-Z3 3 ' UTR contains functional binding sites for both let-7 and miR-125 that confers sensitivity to both of these microRNAs, as determined by deletion analysis in reporter assays. Forced expression of let-7 and miR-125 miRNAs leads to early silencing of Br-Z3 and Chinmo and is associated with inappropriate neuronal sprouting and outgrowth. Similar phenotypes were observed by the combined but not separate depletion of br-Z3 and . Because persistent Br-Z3 was not detected in let-7-C mutants, this work suggests a model in which let-7 and miR-125 activation at the onset of metamorphosis may act as a failsafe mechanism that ensures the coordinated silencing of both br-Z3 and needed for the timely outgrowth of neurons arrested during larval development. The let-7 and miR-125 binding site sequences are conserved across Drosophila species and possibly other insects as well, suggesting that this functional relationship is evolutionarily conserved.}, keywords = {let-7; miR-125; heterochronic; temporal identity; Chinmo; Br-Z3}, year = {2020}, eissn = {2160-1836}, pages = {1393-1401}, orcid-numbers = {Chawla, Geetanjali/0000-0003-0354-3716} } @article{MTMT:30905886, title = {Kruppel homolog 1 and E93: The doorkeeper and the key to insect metamorphosis}, url = {https://m2.mtmt.hu/api/publication/30905886}, author = {Belles, Xavier}, doi = {10.1002/arch.21609}, journal-iso = {ARCH INSECT BIOCHEM}, journal = {ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY}, unique-id = {30905886}, issn = {0739-4462}, abstract = {Insect metamorphosis is regulated by two main hormones: ecdysone (20E), which promotes molting, and juvenile hormone (JH), which inhibits adult morphogenesis. The transduction mechanisms for the respective hormonal signals include the transcription factors Kruppel homolog 1 (Kr-h1) and E93, which are JH- and 20E-dependent, respectively. Kr-h1 is the main effector of the antimetamorphic action of JH, while E93 is a key promoter of metamorphosis. The ancestral regulatory axis of metamorphosis, which operates in insects with hemimetabolan (gradual) metamorphosis and is known as the MEKRE93 pathway, is based on Kr-h1 repression of E93. In the last juvenile stage, when the production of JH dramatically decreases, Kr-h1 expression is almost completely interrupted, E93 becomes upregulated and metamorphosis proceeds. The holometabolan (complete) metamorphosis mode of development includes the peculiar pupal stage, a sort of intermediate between the final larval instar and the adult stage. In holometabolan species, Broad-Complex (BR-C) transcription factors determine the pupal stage and E93 stimulates the expression of BR-C in the prepupa. The MEKRE93 pathway is conserved in holometabolan insects, which have added the E93/BR-C interaction loop to the ancestral (hemimetabolan) pathway during the evolution from hemimetaboly to holometaboly.}, keywords = {ecdysone; metamorphosis; Juvenile hormone; E93; Broad-Complex; Kruppel homolog 1}, year = {2019}, eissn = {1520-6327}, orcid-numbers = {Belles, Xavier/0000-0002-1566-303X} } @article{MTMT:30905894, title = {Upregulation of E93 Gene Expression Acts as the Trigger for Metamorphosis Independently of the Threshold Size in the Beetle Tribolium castaneum}, url = {https://m2.mtmt.hu/api/publication/30905894}, author = {Chafino, Silvia and Urena, Enric and Casanova, Jordi and Casacuberta, Elena and Franch-Marro, Xavier and Martin, David}, doi = {10.1016/j.celrep.2019.03.094}, journal-iso = {CELL REP}, journal = {CELL REPORTS}, volume = {27}, unique-id = {30905894}, issn = {2211-1247}, abstract = {Body size in holometabolous insects is determined by the size at which the juvenile larva undergoes metamorphosis to the pupal stage. To undergo larva-pupa transition, larva must reach a critical developmental checkpoint, the threshold size (TS); however, the molecular mechanisms through which the TS cues this transition remain to be fully characterized. Here, we use the flour beetle Tribolium castaneum to characterize the molecular mechanisms underlying entry into metamorphosis. We found that T. castaneum reaches a TS at the beginning of the last larval instar, which is associated with the downregulation of TcKr-h1 and the upregulation of TcE93 and TcBr-C. Unexpectedly, we found that while there is an association between TS and TcE93 upregulation, it is the latter that constitutes the molecular trigger for metamorphosis initiation. In light of our results, we evaluate the interactions that control the larva-pupa transition and suggest alternative models.}, year = {2019}, eissn = {2211-1247}, pages = {1039-+}, orcid-numbers = {Casanova, Jordi/0000-0001-6121-8589} } @article{MTMT:30905888, title = {Molecular cloning, expression profiling, and functional analysis of a broad-complex isoform 2/3 (Br-Z2/Z3) transcription factor in the diamondback moth, Plutella xylostella (L.)}, url = {https://m2.mtmt.hu/api/publication/30905888}, author = {Huang, Jingfei and Fang, Ling and Wang, Shuai and Liu, Xiang and Chen, Yong and Chen, Yixin and Tian, Houjun and Lin, Shuo and Tian, Sufen and Wei, Hui and Gu, Xiaojun}, doi = {10.1002/arch.21549}, journal-iso = {ARCH INSECT BIOCHEM}, journal = {ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY}, volume = {101}, unique-id = {30905888}, issn = {0739-4462}, abstract = {The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a widespread and destructive pest of cruciferous crops. New strategies for controlling it are needed because it is rapidly developing resistance to conventional pesticides. In insects, transcription factors (TFs) including broad-complex (Br-C) are thought to be useful for insecticide development because they are able to regulate the transcription of functional genes involved in responses to external stimuli including insecticides. In the present study, we cloned and sequenced the open reading frames (ORFs) of three BTB-ZF encoding genes from the diamondback moth deposited in the National Center for Biotechnology Information (NCBI) database under accessions MG753773, MG288674, and MG753772. The lengths of these ORFs were 1,680, 1,428, and 1,647 bp, respectively. The phylogenetic analysis based on the predicted amino acid sequences of ZF domains showed that MG753773 and MG288674 belonged to Z2/Z3 and Z7 of Br-C while MG753772 belonged to Ttk types. In the agreement, the highest expression level of MG753773 occurred during the prepupal stage, MG288674 and MG753772 were expressed during all stages and peaked in the adult and egg stages, respectively. RNA interference silencing of MG753773 in the late third instar larvae significantly decreased survival and pupation of the insects. With precocene II, transcription of MG753773 increased (4x) in the fourth instar larva 24 hr later; 48 hr later the rate of prepupation and pupation was significantly higher. These findings will contribute to the development of new regulators of the growth and development for diamondback moth control.}, keywords = {RNA Interference; metamorphosis; open reading frame; Expression profiling; Plutella xylostella; broad-complex isoform 2/3; precocene II}, year = {2019}, eissn = {1520-6327} } @article{MTMT:30509203, title = {The crustacean ecdysone cassette: A gatekeeper for molt and metamorphosis}, url = {https://m2.mtmt.hu/api/publication/30509203}, author = {Hyde, Cameron J. and Elizur, Abigail and Ventura, Tomer}, doi = {10.1016/j.jsbmb.2018.08.012}, journal-iso = {J STEROID BIOCHEM MOL BIOL}, journal = {JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {185}, unique-id = {30509203}, issn = {0960-0760}, abstract = {Arthropods have long been utilized as models to explore molecular function, and the findings derived from them can be applied throughout metazoa, including as a basis for medical research. This has led to the adoption of many representative insect models beyond Drosophila, as each lends its own unique perspective to questions in endocrinology and genetics. However, non-insect arthropods are yet to be realised for the potential insight they may provide in such studies. The Crustacea are among the most ancient arthropods from which insects descended, comprising a huge variety of life histories and ecological roles. Of the events in a typical crustacean development, metamorphosis is perhaps the most ubiquitous, challenging and highly studied. Despite this, our knowledge of the endocrinology which underpins metamorphosis is rudimentary at best; although several key molecules have been identified and studied in depth, the link between them is quite nebulous and leans heavily on well-explored insect models, which diverged from the Pancrustacea over 450 million years ago. As omics technologies become increasingly accessible, they bring the prospect of explorative molecular research which will allow us to uncover components and pathways unique to crustaceans. This review reconciles known components of crustacean metamorphosis and reflects on our findings in insects to outline a future search space, with focus given to the ecdysone cascade. To expand our knowledge of this ubiquitous endocrine system not only aids in our understanding of crustacean metamorphosis, but also provides a deeper insight into the adaptive capacity of arthropods throughout evolution.}, keywords = {ENDOCRINE; ecdysone; arthropod; molt; Nuclear receptor; Methyl farnesoate}, year = {2019}, eissn = {1879-1220}, pages = {172-183}, orcid-numbers = {Elizur, Abigail/0000-0002-2960-302X; Ventura, Tomer/0000-0002-0777-2367} } @article{MTMT:30905883, title = {Where did the pupa come from? The timing of juvenile hormone signalling supports homology between stages of hemimetabolous and holometabolous insects}, url = {https://m2.mtmt.hu/api/publication/30905883}, author = {Jindra, Marek}, doi = {10.1098/rstb.2019.0064}, journal-iso = {PHILOS T ROY SOC B}, journal = {PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B - BIOLOGICAL SCIENCES}, volume = {374}, unique-id = {30905883}, issn = {0962-8436}, abstract = {Insect metamorphosis boasts spectacular cases of postembryonic development when juveniles undergo massive morphogenesis before attaining the adult form and function; in moths or flies the larvae do not even remotely resemble their adult parents. A selective advantage of complete metamorphosis (holometaboly) is that within one species the two forms with different lifestyles can exploit diverse habitats. It was the environmental adaptation and specialization of larvae, primarily the delay and internalization of wing development, that eventually required an intermediate stage that we call a pupa. It is a long-held and parsimonious hypothesis that the holometabolous pupa evolved through modification of a final juvenile stage of an ancestor developing through incomplete metamorphosis (hemimetaboly). Alternative hypotheses see the pupa as an equivalent of all hemimetabolous moulting cycles (instars) collapsed into one, and consider any preceding holometabolous larval instars free-living embryos stalled in development. Discoveries on juvenile hormone signalling that controls metamorphosis grant new support to the former hypothesis deriving the pupa from a final pre-adult stage. The timing of expression of genes that repress and promote adult development downstream of hormonal signals supports homology between postembryonic stages of hemimetabolous and holometabolous insects.This article is part of the theme issue 'The evolution of complete metamorphosis'.}, keywords = {TRANSCRIPTION FACTOR; EVOLUTION; signal transduction; metamorphosis; hormone receptor; Juvenile hormone}, year = {2019}, eissn = {1471-2970}, orcid-numbers = {Jindra, Marek/0000-0002-2196-9924} } @article{MTMT:30905881, title = {The evolution of insect metamorphosis: a developmental and endocrine view}, url = {https://m2.mtmt.hu/api/publication/30905881}, author = {Truman, James W. and Riddiford, Lynn M.}, doi = {10.1098/rstb.2019.0070}, journal-iso = {PHILOS T ROY SOC B}, journal = {PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B - BIOLOGICAL SCIENCES}, volume = {374}, unique-id = {30905881}, issn = {0962-8436}, abstract = {Developmental, genetic and endocrine data from diverse taxa provide insight into the evolution of insect metamorphosis. We equate the larva-pupa-adult of the Holometabola to the pronymph-nymph-adult of hemimetabolous insects. The hemimetabolous pronymph is a cryptic embryonic stage with unique endocrinology and behavioural modifications that probably served as preadaptations for the larva. It develops in the absence of juvenile hormone (JH) as embryonic primordia undergo patterning and morphogenesis, the processes that were arrested for the evolution of the larva. Embryonic JH then drives tissue differentiation and nymph formation. Experimental treatment of pronymphs with JH terminates patterning and induces differentiation, mimicking the processes that occurred during the evolution of the larva. Unpatterned portions of primordia persist in the larva, becoming imaginal discs that form pupal and adult structures. Key transcription factors are associated with the holometabolous life stages: Kruppel-homolog 1 (Kr-h1) in the larva, broad in the pupa and E93 in the adult. Kr-h1 mediates JH action and is found whenever JH acts, while the other two genes direct the formation of their corresponding stages. In hemimetabolous forms, the pronymph has low Broad expression, followed by Broad expression through the nymphal moults, then a switch to E93 to form the adult.This article is part of the theme issue 'The evolution of complete metamorphosis'.}, keywords = {BROAD; Juvenile hormone; E93; Kruppel-homolog 1; pronymph}, year = {2019}, eissn = {1471-2970} } @article{MTMT:31403688, title = {The evolution of insect metamorphosis}, url = {https://m2.mtmt.hu/api/publication/31403688}, author = {Truman, JW}, doi = {10.1016/j.cub.2019.10.009}, journal-iso = {CURR BIOL}, journal = {CURRENT BIOLOGY}, volume = {29}, unique-id = {31403688}, issn = {0960-9822}, year = {2019}, eissn = {1879-0445}, pages = {R1252-R1268} } @article{MTMT:30905891, title = {Direct and widespread role for the nuclear receptor EcR in mediating the response to ecdysone in Drosophila}, url = {https://m2.mtmt.hu/api/publication/30905891}, author = {Uyehara, Christopher M. and McKay, Daniel J.}, doi = {10.1073/pnas.1900343116}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {116}, unique-id = {30905891}, issn = {0027-8424}, abstract = {The ecdysone pathway was among the first experimental systems employed to study the impact of steroid hormones on the genome. In Drosophila and other insects, ecdysone coordinates developmental transitions, including wholesale transformation of the larva into the adult during metamorphosis. Like other hormones, ecdysone controls gene expression through a nuclear receptor, which functions as a ligand-dependent transcription factor. Although it is clear that ecdysone elicits distinct transcriptional responses within its different target tissues, the role of its receptor, EcR, in regulating target gene expression is incompletely understood. In particular, EcR initiates a cascade of transcription factor expression in response to ecdysone, making it unclear which ecdysone-responsive genes are direct EcR targets. Here, we use the larval-to-prepupal transition of developing wings to examine the role of EcR in gene regulation. Genome-wide DNA binding profiles reveal that EcR exhibits widespread binding across the genome, including at many canonical ecdysone response genes. However, the majority of its binding sites reside at genes with wing-specific functions. We also find that EcR binding is temporally dynamic, with thousands of binding sites changing over time. RNA-seq reveals that EcR acts as both a temporal gate to block precocious entry to the next developmental stage as well as a temporal trigger to promote the subsequent program. Finally, transgenic reporter analysis indicates that EcR regulates not only temporal changes in target enhancer activity but also spatial patterns. Together, these studies define EcR as a multipurpose, direct regulator of gene expression, greatly expanding its role in coordinating developmental transitions.}, keywords = {HORMONE; TRANSCRIPTION FACTOR; ecdysone; CUT&RUN; temporal gene regulation}, year = {2019}, eissn = {1091-6490}, pages = {9893-9902} } @article{MTMT:30509201, title = {E93 expression and links to the juvenile hormone in hemipteran mealybugs with insights on female neoteny}, url = {https://m2.mtmt.hu/api/publication/30509201}, author = {Vea, Isabelle Mifom and Tanaka, Sayumi and Tsuji, Tomohiro and Shiotsuki, Takahiro and Jouraku, Akiya and Minakuchi, Chieka}, doi = {10.1016/j.ibmb.2018.11.008}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {104}, unique-id = {30509201}, issn = {0965-1748}, abstract = {Insect metamorphosis produces reproductive adults and is commonly accompanied with the direct or indirect development of wings. In some winged insects, the imago is altered by life history changes. For instance, in scale insects and mealybugs, reproductive females retain juvenile features and are wingless. The transcription factor E93 triggers metamorphosis and plays in concert with the juvenile hormone pathway to guarantee the successful transition from juvenile to adult. We previously provided evidence of an atypical down-regulation of the juvenile hormone pathway during female development in the Japanese mealybug. Here, we further investigate how E93 is involved in the production of neotenic wingless females, by identifying its isoforms, assessing their expression patterns and evaluating the effect of exogenous juvenile hormone mimic treatment on E93. This study identifies three E93 isoforms on the 5' end, based on Japanese mealybug cDNA and shows that female development occurs with the near absence of E93 transcripts, as opposed to male metamorphosis. Additionally, while male development is typically affected by exogenous juvenile hormone mimic treatments, females seem to remain insensitive to the treatment, and up-regulation of the juvenile hormone signaling is not observed. Furthermore, juvenile hormone mimic treatment on female nymphs did not have an obvious effect on E93 transcription, while treatment on male prepupae resulted in depleted E93 transcripts. In this study, we emphasize the importance in examining atypical cases of metamorphosis as complementary systems to provide a better understanding on the molecular mechanisms underlying insect metamorphosis. For instance, the factors regulating the expression of E93 are largely unclear. Investigating the regulatory mechanism of E93 transcription could provide clues towards identifying the factors that induce or suppress E93 transcription, in turn triggering male adult development or female neoteny.}, keywords = {Juvenile hormone; E93; Insect metamorphosis; Neoteny; Hemimetaboly; Mealybugs}, year = {2019}, eissn = {1879-0240}, pages = {65-72} } @article{MTMT:30902363, title = {Impairment of pupation by RNA interference-aided knockdown of Broad-Complex gene in Leptinotarsa decemlineata (Say)}, url = {https://m2.mtmt.hu/api/publication/30902363}, author = {Xu, Q-Y and Meng, Q-W and Deng, P. and Fu, K-Y and Guo, W-C and Li, G-Q}, doi = {10.1017/S0007485318001050}, journal-iso = {B ENTOMOL RES}, journal = {BULLETIN OF ENTOMOLOGICAL RESEARCH}, volume = {109}, unique-id = {30902363}, issn = {0007-4853}, abstract = {Dietary delivery of bacterially expressed double-stranded RNA (dsRNA) has a great potential for management of Leptinotarsa decemlineata. An important first step is to discover possible RNA-interference (RNAi)-target genes effective against larvae, especially the old larvae. In the present paper, five putative Broad-Complex (BrC) cDNAs (Z1-Z4, and Z6) were identified in L. decemlineata. The expression of the five LdBrC isoforms was suppressed by juvenile hormone signaling, whereas the transcription was upregulated by 20-hydroxyecdysone signaling at the fourth (final) instar larval stage. Feeding of bacterially expressed dsBrC (derived from a common fragment of the five LdBrC variants) in the third- and fourth-instar larvae successfully knocked down the target mRNAs. For the fourth-instar LdBrC RNAi hypomorphs, they had a higher larval mortality compared with the controls. Moreover, most dsBrC-fed beetles did not pupate normally. After removal of the apolysed larval cuticle, a miniature adult was found. The adult head, compound eyes, prothorax, mesothorax, metathorax were found on the dorsal view. Distinct adult cuticle pigmentation was seen on the prothorax. The mouthparts, forelegs, midlegs, and hindlegs could be observed on the ventral view of the miniature adults. For the third-instar LdBrC RNAi specimens, around 20% moribund beetles remained as prepupae and finally died. Therefore, LdBrC is among the most attractive candidate genes for RNAi to control the fourth-instar larvae in L. decemlineata.}, keywords = {RNA Interference; metamorphosis; Leptinotarsa decemlineata; Broad Complex; pupation}, year = {2019}, eissn = {1475-2670}, pages = {659-668} } @article{MTMT:30902364, title = {Isoform specific roles of Broad-Complex in larval development in Leptinotarsa decemlineata}, url = {https://m2.mtmt.hu/api/publication/30902364}, author = {Xu, Q. -Y. and Meng, Q. -W. and Deng, P. and Fu, K. -Y. and Guo, W. -C. and Li, G. -Q.}, doi = {10.1111/imb.12563}, journal-iso = {INSECT MOL BIOL}, journal = {INSECT MOLECULAR BIOLOGY}, volume = {28}, unique-id = {30902364}, issn = {0962-1075}, abstract = {Broad-Complex (BrC) is a downstream target of both 20-hydroxyecdysone and juvenile hormone signalling. BrC regulates morphogenetic changes between nymphal instars in hemimetabolans, whereas it controls pupal commitment, pupal morphogenesis and inhibits adult differentiation in holometabolans. Among five BrC cDNAs (Z1-Z4 and Z6) identified in the Colorado potato beetle, we found in this work that Z1, Z2 and Z6 were mainly expressed at the last (fourth) instar and prepupal stages, whereas the levels of Z3 and Z4 increased during the penultimate (third) instar stage, peaked at the last instar larval phase and gradually decreased at the prepupal and pupal periods. When knocking down all BrC isoforms by RNA interference (RNAi) at the penultimate instar stage, around 20% of the resultant larvae remained as moribund beetles. These moribund BrC RNAi larvae were completely or partially wrapped in old cuticle. Likewise, a portion of larvae treated for a single double-stranded RNA of Z3, Z4 or Z6 displayed a degree of similar aberrancies, increasing in the order of isoforms Z6 BrC isoforms at the last instar period, most of the RNAi larvae did not normally pupate or emerge as adults. Separately silencing each of the five zinc finger domains revealed that approximately 70% of the Z1 RNAi larvae remained as prepupae, around 60% of the Z6 RNAi specimens formed aberrant prepupae or pupae and about 60% of the Z2 RNAi beetles became deformed pupae. After removal of the old exuviae, these deformed larvae in which either Z1, Z2 or Z6 was depleted possessed adult prothorax and mesothorax, developing antenna, mouthparts and wing discs. Moreover, less than 50% of the resultant pupae finally emerged as adults when either of Z1, Z2 or Z6 was knocked down. Therefore, our findings reveal, for the first time, that the two roles of BrC in insect groups (ie directing morphogenetic changes during juvenile development and regulating larval-pupal-adult metamorphosis) are played by different BrC isoforms in Leptinotarsa decemlineata.}, keywords = {metamorphosis; Leptinotarsa decemlineata; pupation; Broad-Complex}, year = {2019}, eissn = {1365-2583}, pages = {420-430} } @article{MTMT:27525173, title = {Apical and Basal Matrix Remodeling Control Epithelial Morphogenesis}, url = {https://m2.mtmt.hu/api/publication/27525173}, author = {Diaz-de-la-Loza, Maria-del-Carmen and Ray, Robert P and Ganguly, Poulami S and Alt, Silvanus and Davis, John R and Hoppe, Andreas and Tapon, Nic and Salbreux, Guillaume and Thompson, Barry J}, doi = {10.1016/j.devcel.2018.06.006}, journal-iso = {DEV CELL}, journal = {DEVELOPMENTAL CELL}, volume = {46}, unique-id = {27525173}, issn = {1534-5807}, year = {2018}, eissn = {1878-1551}, pages = {23-+} } @article{MTMT:30714105, title = {Physiological functions and Ca2+ signal transduction pathway exploring during molting of silkworm, Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/30714105}, author = {Li, Wenchu and Xu, Yaling and Zhong, Yangjin and Chen, Liujuan}, doi = {10.1016/j.aspen.2018.10.021}, journal-iso = {J ASIA PAC ENTOMOL}, journal = {JOURNAL OF ASIA PACIFIC ENTOMOLOGY}, volume = {21}, unique-id = {30714105}, issn = {1226-8615}, abstract = {Calcium ions are an important second messagers in the life of organisms, such as in silkworm growth and their developmental processes; these ions can trigger various effects, including the muscle excitation-contraction coupling-mechanism, secretion of endocrine and exocrine glands, neuronal activities, midgut nutrients metabolisms, sensory system, enzymatic systems, and acceleration of metabolism activities. This paper reviewed the ecdysis process and mechanisms, where calcium plays an important role in larvae-to-larvae ecdysis and larvae-to-pupae and pupae-to-moth metamorphosis of silkworms; the paper focuses on the advanced achievements in the field of calcium actions during larvae-to-larvae and larvae-to-pupae changes. Calcium ion channels are shown to be the action mechanism of phospholipase C in the G protein cascade of the calcium signal transduction pathway, and the activators and inhibitors of these calcium channels are further discussed.}, keywords = {Calcium ion; Ecdysis; signal transduction pathway; Bombyx mori; Physiological function}, year = {2018}, eissn = {1876-7990}, pages = {1457-1465} } @article{MTMT:30465803, title = {Ecdysteroid and juvenile hormone biosynthesis, receptors and their signaling in the freshwater microcrustacean Daphnia}, url = {https://m2.mtmt.hu/api/publication/30465803}, author = {Miyakawa, Hitoshi and Sato, Tomomi and Song, You and Tollefsen, Knut Erik and Iguchi, Taisen}, doi = {10.1016/j.jsbmb.2017.12.006}, journal-iso = {J STEROID BIOCHEM MOL BIOL}, journal = {JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {184}, unique-id = {30465803}, issn = {0960-0760}, abstract = {The two essential insect hormones, ecdysteroids and juvenile hormones, are possessed not only by insects, but also widely by arthropods, and regulate various developmental and physiological processes. In contrast to the abundant information about molecular endocrine mechanisms in insects, the knowledge of non-insect arthropod endocrinology is still limited. In this review, we summarize recent reports about the molecular basis of these to major insect hormones in the freshwater microcrustacean Daphnia, a keystone taxon in limnetic ecology and bioindicator in environmental studies. Comprehensive comparisons of endocrine signaling pathways between insects and daphnids may shed light on the regulatory mechanisms of various biological phenomena an, moreover, evolutionary processes of arthropod species.}, keywords = {ecdysteroid; Daphnia magna; Juvenile hormone; Daphnia pulex; Hormonal crosstalk}, year = {2018}, eissn = {1879-1220}, pages = {62-68} } @article{MTMT:27050162, title = {The Role of MicroRNAs in Drosophila Regulation of Insulin-Like Peptides and Ecdysteroid Signalling: Where Are We Now?}, url = {https://m2.mtmt.hu/api/publication/27050162}, author = {Cao, Jian Q and Tong, Wai S and Yu, Hiu Y and Tobe, Stephen S and Bendena, William G and Hui, Jerome H L}, doi = {10.1016/bs.aiip.2017.02.002}, editor = {Verlinden, H}, journal-iso = {ADV INSECT PHYSIOL}, journal = {ADVANCES IN INSECT PHYSIOLOGY}, volume = {53}, unique-id = {27050162}, issn = {0065-2806}, year = {2017}, eissn = {2213-6800}, pages = {55-85} } @article{MTMT:26537160, title = {Molecular mechanism underlying juvenile hormone-mediated repression of precocious larval-adult metamorphosis}, url = {https://m2.mtmt.hu/api/publication/26537160}, author = {Kayukawa, Takumi and Jouraku, Akiya and Ito, Yuka and Shinoda, Tetsuro}, doi = {10.1073/pnas.1615423114}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {114}, unique-id = {26537160}, issn = {0027-8424}, year = {2017}, eissn = {1091-6490}, pages = {1057-1062} } @article{MTMT:27050161, title = {Dusky works upstream of Four-jointed and Forked in wing morphogenesis in Tribolium castaneum}, url = {https://m2.mtmt.hu/api/publication/27050161}, author = {Li, C and Li, B and Ma, S and Lue, P and Chen, K}, doi = {10.1111/imb.12327}, journal-iso = {INSECT MOL BIOL}, journal = {INSECT MOLECULAR BIOLOGY}, volume = {26}, unique-id = {27050161}, issn = {0962-1075}, year = {2017}, eissn = {1365-2583}, pages = {677-686} } @article{MTMT:26715470, title = {Ecdysone Receptor Agonism Leading to Lethal Molting Disruption in Arthropods: Review and Adverse Outcome Pathway Development}, url = {https://m2.mtmt.hu/api/publication/26715470}, author = {Song, You and Villeneuve, Daniel L and Toyota, Kenji and Iguchi, Taisen and Tollefsen, Knut Erik}, doi = {10.1021/acs.est.7b00480}, journal-iso = {ENVIRON SCI TECHNOL}, journal = {ENVIRONMENTAL SCIENCE & TECHNOLOGY}, volume = {51}, unique-id = {26715470}, issn = {0013-936X}, year = {2017}, eissn = {1520-5851}, pages = {4142-4157} } @article{MTMT:26715469, title = {Hormone-dependent control of developmental timing through regulation of chromatin accessibility}, url = {https://m2.mtmt.hu/api/publication/26715469}, author = {Uyehara, Christopher M and Nystrom, Spencer L and Niederhuber, Matthew J and Leatham-Jensen, Mary and Ma, Yiqin and Buttitta, Laura A and McKay, Daniel J}, doi = {10.1101/gad.298182.117}, journal-iso = {GENE DEV}, journal = {GENES & DEVELOPMENT}, volume = {31}, unique-id = {26715469}, issn = {0890-9369}, year = {2017}, eissn = {1549-5477}, pages = {862-875} } @article{MTMT:27273302, title = {Differentiation of lepidoptera scale cells from epidermal stem cells followed by ecdysone-regulated DNA duplication and scale secreting}, url = {https://m2.mtmt.hu/api/publication/27273302}, author = {Yuan, Shenglei and Huang, Wuren and Geng, Lei and Beerntsen, Brenda T and Song, Hongsheng and Ling, Erjun}, doi = {10.1080/15384101.2017.1376148}, journal-iso = {CELL CYCLE}, journal = {CELL CYCLE}, volume = {16}, unique-id = {27273302}, issn = {1538-4101}, year = {2017}, eissn = {1551-4005}, pages = {2156-2167} } @article{MTMT:26537103, title = {20-hydroxyecdysone enhances the expression of the chitinase 5 via Broad-Complex Zinc-Finger 4 during metamorphosis in silkworm, Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/26537103}, author = {Zhang, X and Zheng, S}, doi = {10.1111/imb.12288}, journal-iso = {INSECT MOL BIOL}, journal = {INSECT MOLECULAR BIOLOGY}, volume = {26}, unique-id = {26537103}, issn = {0962-1075}, year = {2017}, eissn = {1365-2583}, pages = {243-253} } @article{MTMT:26183633, title = {Kruppel Homolog 1 Inhibits Insect Metamorphosis via Direct Transcriptional Repression of Broad-Complex, a Pupal Specifier Gene}, url = {https://m2.mtmt.hu/api/publication/26183633}, author = {Kayukawa, Takumi and Nagamine, Keisuke and Ito, Yuka and Nishita, Yoshinori and Ishikawa, Yukio and Shinoda, Tetsuro}, doi = {10.1074/jbc.M115.686121}, journal-iso = {J BIOL CHEM}, journal = {JOURNAL OF BIOLOGICAL CHEMISTRY}, volume = {291}, unique-id = {26183633}, issn = {0021-9258}, year = {2016}, eissn = {1083-351X}, pages = {1751-1762} } @article{MTMT:26336417, title = {Digitor/dASCIZ has multiple roles in Drosophila development}, url = {https://m2.mtmt.hu/api/publication/26336417}, author = {Sengupta, S and Rath, U and Yao, C and Zavortink, M and Wang, C and Girton, J and Johansen, KM and Johansen, J}, doi = {10.1371/journal.pone.0166829}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {11}, unique-id = {26336417}, issn = {1932-6203}, year = {2016}, eissn = {1932-6203} } @article{MTMT:26051186, title = {The Occurrence of the Holometabolous Pupal Stage Requires the Interaction between E93, Kruppel-Homolog 1 and Broad-Complex}, url = {https://m2.mtmt.hu/api/publication/26051186}, author = {Urena, Enric and Chafino, Silvia and Manjon, Cristina and Franch-Marro, Xavier and Martin, David}, doi = {10.1371/journal.pgen.1006020}, journal-iso = {PLOS GENET}, journal = {PLOS GENETICS}, volume = {12}, unique-id = {26051186}, issn = {1553-7390}, year = {2016}, eissn = {1553-7404} } @article{MTMT:26183632, title = {Differential Juvenile Hormone Variations in Scale Insect Extreme Sexual Dimorphism}, url = {https://m2.mtmt.hu/api/publication/26183632}, author = {Vea, Isabelle Mifom and Tanaka, Sayumi and Shiotsuki, Takahiro and Jouraku, Akiya and Tanaka, Toshiharu and Minakuchi, Chieka}, doi = {10.1371/journal.pone.0149459}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {11}, unique-id = {26183632}, issn = {1932-6203}, year = {2016}, eissn = {1932-6203} } @article{MTMT:26051190, title = {Juvenile Hormone and Broad-Complex Gene Expression on Programmed Cell Death in the Anterior Silk Glands of the Bamboo Borer (Omphisa fuscidentalis Hampson)}, url = {https://m2.mtmt.hu/api/publication/26051190}, author = {Bunkuna, Suparin and Singtripop, Tippawan and Manaboon, Manaporn}, journal-iso = {CHIANG MAI J SCI}, journal = {CHIANG MAI JOURNAL OF SCIENCE}, volume = {42}, unique-id = {26051190}, issn = {0125-2526}, year = {2015}, eissn = {0125-2526}, pages = {126-135} } @article{MTMT:26051189, title = {Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/26051189}, author = {Sap, Karen A and Bezstarosti, Karel and Dekkers, Dick H W and van den Hout, Mirjam and van Ijcken, Wilfred and Rijkers, Erikjan and Demmers, Jeroen A A}, doi = {10.1002/pmic.201400308}, journal-iso = {PROTEOMICS}, journal = {PROTEOMICS}, volume = {15}, unique-id = {26051189}, issn = {1615-9853}, year = {2015}, eissn = {1615-9861}, pages = {725-738} } @article{MTMT:24280604, title = {Juvenile Hormone Prevents 20-Hydroxyecdysone-induced Metamorphosis by Regulating the Phosphorylation of a Newly Identified Broad Protein}, url = {https://m2.mtmt.hu/api/publication/24280604}, author = {Cai, MJ and Liu, W and Pei, XY and Li, XR and He, HJ and Wang, JX and Zhao, XF}, doi = {10.1074/jbc.M114.581876}, journal-iso = {J BIOL CHEM}, journal = {JOURNAL OF BIOLOGICAL CHEMISTRY}, volume = {289}, unique-id = {24280604}, issn = {0021-9258}, year = {2014}, eissn = {1083-351X}, pages = {26630-26641} } @article{MTMT:24722126, title = {The COP9 Signalosome Converts Temporal Hormone Signaling to Spatial Restriction on Neural Competence}, url = {https://m2.mtmt.hu/api/publication/24722126}, author = {Huang, YC and Lu, YN and Wu, JT and Chien, CT and Pi, HW}, doi = {10.1371/journal.pgen.1004760}, journal-iso = {PLOS GENET}, journal = {PLOS GENETICS}, volume = {10}, unique-id = {24722126}, issn = {1553-7390}, year = {2014}, eissn = {1553-7404} } @article{MTMT:24092799, title = {Ecdysone response elements in the distal promoter of the Bombyx Broad-Complex gene, BmBR-C}, url = {https://m2.mtmt.hu/api/publication/24092799}, author = {Nishita, Y}, doi = {10.1111/imb.12085}, journal-iso = {INSECT MOL BIOL}, journal = {INSECT MOLECULAR BIOLOGY}, volume = {23}, unique-id = {24092799}, issn = {0962-1075}, year = {2014}, eissn = {1365-2583}, pages = {341-356} } @article{MTMT:24440760, title = {The Broad Complex isoform 2 (BrC-Z2) transcriptional factor plays a critical role in vitellogenin transcription in the silkworm Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/24440760}, author = {Yang, CW and Lin, Y and Liu, HL and Shen, GW and Luo, J and Zhang, HY and Peng, ZX and Chen, EX and Xing, RM and Han, CS and Xia, QY}, doi = {10.1016/j.bbagen.2014.05.013}, journal-iso = {BBA-GEN SUBJECTS}, journal = {BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS}, volume = {1840}, unique-id = {24440760}, issn = {0304-4165}, year = {2014}, eissn = {1872-8006}, pages = {2674-2684} } @article{MTMT:23406490, title = {Broad-complex functions in postembryonic development of the cockroach Blattella germanica shed new light on the evolution of insect metamorphosis}, url = {https://m2.mtmt.hu/api/publication/23406490}, author = {Huang, JH and Lozano, J and Belles, X}, doi = {10.1016/j.bbagen.2012.09.025}, journal-iso = {BBA-GEN SUBJECTS}, journal = {BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS}, volume = {1830}, unique-id = {23406490}, issn = {0304-4165}, year = {2013}, eissn = {1872-8006}, pages = {2178-2187} } @article{MTMT:24092801, title = {Dynamic feedback circuits function as a switch for shaping a maturation-inducing steroid pulse in Drosophila}, url = {https://m2.mtmt.hu/api/publication/24092801}, author = {Moeller, ME and Danielsen, ET and Herder, R and O'Connor, MB and Rewitz, KF}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {140}, unique-id = {24092801}, issn = {0950-1991}, year = {2013}, eissn = {1477-9129}, pages = {4730-+} } @article{MTMT:23684202, title = {What Goes Up Must Come Down: Transcription Factors Have Their Say in Making Ecdysone Pulses}, url = {https://m2.mtmt.hu/api/publication/23684202}, author = {Ou, QX and King-Jones, K}, doi = {10.1016/B978-0-12-385979-2.00002-2}, journal-iso = {CURR TOP DEV BIOL}, journal = {CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY}, volume = {103}, unique-id = {23684202}, issn = {0070-2153}, year = {2013}, eissn = {1557-8933}, pages = {35-71} } @article{MTMT:23406489, title = {Developmental Checkpoints and Feedback Circuits Time Insect Maturation}, url = {https://m2.mtmt.hu/api/publication/23406489}, author = {Rewitz, KF and Yamanaka, N and O'Connor, MB}, doi = {10.1016/B978-0-12-385979-2.00001-0}, journal-iso = {CURR TOP DEV BIOL}, journal = {CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY}, volume = {103}, unique-id = {23406489}, issn = {0070-2153}, year = {2013}, eissn = {1557-8933}, pages = {1-33} } @article{MTMT:24092800, title = {Subtle roles of microRNAs let-7, miR-100 and miR-125 on wing morphogenesis in hemimetabolan metamorphosis}, url = {https://m2.mtmt.hu/api/publication/24092800}, author = {Rubio, M and Belles, X}, doi = {10.1016/j.jinsphys.2013.09.003}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {59}, unique-id = {24092800}, issn = {0022-1910}, year = {2013}, eissn = {1879-1611}, pages = {1089-1094} } @article{MTMT:23406491, title = {Tissue Damage Disrupts Developmental Progression and Ecdysteroid Biosynthesis in Drosophila}, url = {https://m2.mtmt.hu/api/publication/23406491}, author = {Hackney, JF and Zolali-Meybodi, O and Cherbas, P}, doi = {10.1371/journal.pone.0049105}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {7}, unique-id = {23406491}, issn = {1932-6203}, year = {2012}, eissn = {1932-6203} } @article{MTMT:23406492, title = {The Effect of Diazinon on Puffing Activity in Polytene Chromosomes of Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/23406492}, author = {Karatas, A and Bahceci, Z and Baspinar, E}, doi = {10.5053/ekoloji.2012.8511}, journal-iso = {EKOLOJI}, journal = {EKOLOJI}, volume = {21}, unique-id = {23406492}, issn = {1300-1361}, year = {2012}, pages = {98-106} } @article{MTMT:23406493, title = {Broad relays hormone signals to regulate stem cell differentiation in Drosophila midgut during metamorphosis}, url = {https://m2.mtmt.hu/api/publication/23406493}, author = {Zeng, XK and Hou, SX}, doi = {10.1242/dev.083030}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {139}, unique-id = {23406493}, issn = {0950-1991}, year = {2012}, eissn = {1477-9129}, pages = {3917-3925} } @article{MTMT:22104027, title = {Drosophila Met and Gce are partially redundant in transducing juvenile hormone action}, url = {https://m2.mtmt.hu/api/publication/22104027}, author = {Abdou, MA and He, QY and Wen, D and Zyaan, O and Wang, J and Xu, JJ and Baumann, AA and Joseph, J and Wilson, TG and Li, S and Wang, J}, doi = {10.1016/j.ibmb.2011.09.003}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {41}, unique-id = {22104027}, issn = {0965-1748}, year = {2011}, eissn = {1879-0240}, pages = {938-945} } @article{MTMT:23406494, title = {Common and Distinct Roles of Juvenile Hormone Signaling Genes in Metamorphosis of Holometabolous and Hemimetabolous Insects}, url = {https://m2.mtmt.hu/api/publication/23406494}, author = {Konopova, B and Smykal, V and Jindra, M}, doi = {10.1371/journal.pone.0028728}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {6}, unique-id = {23406494}, issn = {1932-6203}, year = {2011}, eissn = {1932-6203} } @article{MTMT:22189138, title = {Role of Elongator Subunit Elp3 in Drosophila melanogaster Larval Development and Immunity}, url = {https://m2.mtmt.hu/api/publication/22189138}, author = {Walker, J and Kwon, SY and Badenhorst, P and East, P and McNeill, H and Svejstrup, JQ}, doi = {10.1534/genetics.110.123893}, journal-iso = {GENETICS}, journal = {GENETICS}, volume = {187}, unique-id = {22189138}, issn = {0016-6731}, year = {2011}, eissn = {1943-2631}, pages = {1067-1075} } @article{MTMT:22104029, title = {Tissue-autonomous EcR functions are required for concurrent organ morphogenesis in the Drosophila embryo}, url = {https://m2.mtmt.hu/api/publication/22104029}, author = {Chavoshi, TM and Moussian, B and Uv, A}, doi = {10.1016/j.mod.2010.01.003}, journal-iso = {MECH DEVELOP}, journal = {MECHANISMS OF DEVELOPMENT}, volume = {127}, unique-id = {22104029}, issn = {0925-4773}, year = {2010}, eissn = {1872-6356}, pages = {308-319} } @article{MTMT:22104030, title = {The role of Drosophila Lamin C in muscle function and gene expression}, url = {https://m2.mtmt.hu/api/publication/22104030}, author = {Dialynas, G and Speese, S and Budnik, V and Geyer, PK and Wallrath, LL}, doi = {10.1242/dev.048231}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {137}, unique-id = {22104030}, issn = {0950-1991}, year = {2010}, eissn = {1477-9129}, pages = {3067-3077} } @article{MTMT:22104032, title = {Developmental expression of mRNAs for epidermal and fat body proteins and hormonally regulated transcription factors in the tobacco hornworm, Manduca sexta}, url = {https://m2.mtmt.hu/api/publication/22104032}, author = {Hiruma, K and Riddiford, LM}, doi = {10.1016/j.jinsphys.2010.03.029}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {56}, unique-id = {22104032}, issn = {0022-1910}, year = {2010}, eissn = {1879-1611}, pages = {1390-1395} } @article{MTMT:23422790, title = {Cyclin Y Is a Novel Conserved Cyclin Essential for Development in Drosophila}, url = {https://m2.mtmt.hu/api/publication/23422790}, author = {Liu, DM and Finley, RL}, doi = {10.1534/genetics.110.114017}, journal-iso = {GENETICS}, journal = {GENETICS}, volume = {184}, unique-id = {23422790}, issn = {0016-6731}, year = {2010}, eissn = {1943-2631}, pages = {1025-U232} } @article{MTMT:22193535, title = {Why cyclin Y? A highly conserved cyclin with essential functions}, url = {https://m2.mtmt.hu/api/publication/22193535}, author = {Liu, DM and Guest, S and Finley, RL}, doi = {10.4161/fly.4.4.12881}, journal-iso = {FLY}, journal = {FLY}, volume = {4}, unique-id = {22193535}, issn = {1933-6934}, year = {2010}, eissn = {1933-6942}, pages = {278-282} } @article{MTMT:22104031, title = {Key roles of the Broad-Complex gene in insect embryogenesis}, url = {https://m2.mtmt.hu/api/publication/22104031}, author = {Piulachs, MD and Pagone, V and Belles, X}, doi = {10.1016/j.ibmb.2010.04.006}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {40}, unique-id = {22104031}, issn = {0965-1748}, year = {2010}, eissn = {1879-0240}, pages = {468-475} } @article{MTMT:22104035, title = {Ecr-b1 and usp nuclear hormone receptors regulate expression of the vm32e eggshell gene during drosophila oogenesis}, url = {https://m2.mtmt.hu/api/publication/22104035}, author = {Bernardi, F and Romani, P and Tzertzinis, G and Gargiulo, G and Cavaliere, V}, doi = {10.1016/j.ydbio.2009.01.013}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {328}, unique-id = {22104035}, issn = {0012-1606}, year = {2009}, eissn = {1095-564X}, pages = {541-551} } @article{MTMT:22104034, title = {Elucidation of the regulation of an adult cuticle gene acp65a by the transcription factor broad}, url = {https://m2.mtmt.hu/api/publication/22104034}, author = {Cui, HY and Lestradet, M and Bruey-Sedano, N and Charles, JP and Riddiford, LM}, doi = {10.1111/j.1365-2583.2009.00889.x}, journal-iso = {INSECT MOL BIOL}, journal = {INSECT MOLECULAR BIOLOGY}, volume = {18}, unique-id = {22104034}, issn = {0962-1075}, year = {2009}, eissn = {1365-2583}, pages = {421-429} } @article{MTMT:22104036, title = {Broad complex isoforms have unique distributions during central nervous system metamorphosis in drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/22104036}, author = {Spokony, RF and Restifo, LL}, doi = {10.1002/cne.22119}, journal-iso = {J COMP NEUROL}, journal = {JOURNAL OF COMPARATIVE NEUROLOGY}, volume = {517}, unique-id = {22104036}, issn = {0021-9967}, year = {2009}, eissn = {1096-9861}, pages = {15-36} } @article{MTMT:22232168, title = {Activation of BMWCP10 promoter and regulation by BR-C Z2 in wing disc of Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/22232168}, author = {Wang, HB and Iwanaga, M and Kawasaki, H}, doi = {10.1016/j.ibmb.2009.06.008}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {39}, unique-id = {22232168}, issn = {0965-1748}, year = {2009}, eissn = {1879-0240}, pages = {615-623} } @article{MTMT:22104043, title = {Broad-complex acts downstream of Met in juvenile hormone signaling to coordinate primitive Holometabolan metamorphosis}, url = {https://m2.mtmt.hu/api/publication/22104043}, author = {Konopova, B and Jindra, M}, doi = {10.1242/dev.016097}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {135}, unique-id = {22104043}, issn = {0950-1991}, year = {2008}, eissn = {1477-9129}, pages = {559-568} } @article{MTMT:22104041, title = {Kruppel homolog 1 (Kr-h1) mediates juvenile hormone action during metamorphosis of Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/22104041}, author = {Minakuchi, C and Zhou, X and Riddiford, LM}, doi = {10.1016/j.mod.2007.10.002}, journal-iso = {MECH DEVELOP}, journal = {MECHANISMS OF DEVELOPMENT}, volume = {125}, unique-id = {22104041}, issn = {0925-4773}, year = {2008}, eissn = {1872-6356}, pages = {91-105} } @article{MTMT:22104040, title = {The role of 20-hydroxyecdysone and juvenile hormone in pupal commitment of the epidermis of the silkworm, Bombyx mori}, url = {https://m2.mtmt.hu/api/publication/22104040}, author = {Muramatsu, D and Kinjoh, T and Shinoda, T and Hiruma, K}, doi = {10.1016/j.mod.2008.02.001}, journal-iso = {MECH DEVELOP}, journal = {MECHANISMS OF DEVELOPMENT}, volume = {125}, unique-id = {22104040}, issn = {0925-4773}, year = {2008}, eissn = {1872-6356}, pages = {411-420} } @article{MTMT:24519438, title = {Transcription factor broad suppresses precocious development of adult structures during larval-pupal metamorphosis in the red flour beetle, Tribolium castaneum}, url = {https://m2.mtmt.hu/api/publication/24519438}, author = {Parthasarathy, R and Tan, A and Bai, H and Palli, SR}, doi = {10.1016/j.mod.2007.11.001}, journal-iso = {MECH DEVELOP}, journal = {MECHANISMS OF DEVELOPMENT}, volume = {125}, unique-id = {24519438}, issn = {0925-4773}, year = {2008}, eissn = {1872-6356}, pages = {299-313} } @article{MTMT:22104039, title = {The role of broad in the development of Tribolium castaneum: implications for the evolution of the holometabolous insect pupa}, url = {https://m2.mtmt.hu/api/publication/22104039}, author = {Suzuki, Y and Truman, JW and Riddiford, LM}, doi = {10.1242/dev.015263}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {135}, unique-id = {22104039}, issn = {0950-1991}, year = {2008}, eissn = {1477-9129}, pages = {569-577} } @article{MTMT:22104042, title = {Cad74A is regulated by BR and is required for robust dorsal appendage formation in Drosophila oogenesis}, url = {https://m2.mtmt.hu/api/publication/22104042}, author = {Zartman, JJ and Yakoby, N and Bristow, CA and Zhou, X and Schlichting, K and Dahmann, C and Shvartsman, SY}, doi = {10.1016/j.ydbio.2008.07.027}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {322}, unique-id = {22104042}, issn = {0012-1606}, year = {2008}, eissn = {1095-564X}, pages = {289-301} } @article{MTMT:22104046, title = {Anciently duplicated Broad Complex exons have distinct temporal functions during tissue morphogenesis}, url = {https://m2.mtmt.hu/api/publication/22104046}, author = {Spokony, RF and Restifo, LL}, doi = {10.1007/s00427-007-0159-y}, journal-iso = {DEV GENES EVOL}, journal = {DEVELOPMENT GENES AND EVOLUTION}, volume = {217}, unique-id = {22104046}, issn = {0949-944X}, year = {2007}, eissn = {1432-041X}, pages = {499-513} } @article{MTMT:22104044, title = {Experimental selection for drosophila survival in extremely low o-2 environment}, url = {https://m2.mtmt.hu/api/publication/22104044}, author = {Zhou, D and Xue, J and Chen, JM and Morcillo, P and Lambert, JD and White, KP and Haddad, GG}, doi = {10.1371/journal.pone.0000490}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {2}, unique-id = {22104044}, issn = {1932-6203}, year = {2007}, eissn = {1932-6203}, pages = {e490} } @article{MTMT:22104045, title = {Distinct Roles of Broad Isoforms in Regulation of The 20-hydroxyecdysone Effector Gene, Vitellogenin, in The Mosquito Aedes Aegypti}, url = {https://m2.mtmt.hu/api/publication/22104045}, author = {Zhu, JS and Chen, L and Raikhel, AS}, doi = {10.1016/j.mce.2007.01.006}, journal-iso = {MOL CELL ENDOCRINOL}, journal = {MOLECULAR AND CELLULAR ENDOCRINOLOGY}, volume = {267}, unique-id = {22104045}, issn = {0303-7207}, year = {2007}, eissn = {1872-8057}, pages = {97-105} } @article{MTMT:23389046, title = {Broad-Complex, E74, and E75 early genes control DNA puff BhC4-1 expression in prepupal salivary glands}, url = {https://m2.mtmt.hu/api/publication/23389046}, author = {Basso, LR and Neves, MD and Monesi, N and Paco-Larson, ML}, doi = {10.1002/dvg.20239}, journal-iso = {GENESIS}, journal = {GENESIS: THE JOURNAL OF GENETICS AND DEVELOPMENT}, volume = {44}, unique-id = {23389046}, issn = {1526-954X}, year = {2006}, eissn = {1526-968X}, pages = {505-514} } @article{MTMT:22104048, title = {how functions in leg development during Drosophila metamorphosis}, url = {https://m2.mtmt.hu/api/publication/22104048}, author = {Fortier, T M and Chatterjee, R and Klinedinst, S and Baehrecke, E H and Woodard, C T}, doi = {10.1002/dvdy.20852}, journal-iso = {DEV DYNAM}, journal = {DEVELOPMENTAL DYNAMICS}, volume = {235}, unique-id = {22104048}, issn = {1058-8388}, year = {2006}, eissn = {1097-0177}, pages = {2248-2259} } @article{MTMT:22104049, title = {Functional role of aspartic proteinase cathepsin D in insect metamorphosis}, url = {https://m2.mtmt.hu/api/publication/22104049}, author = {Gui, Z Z and Lee, K S and Kim, B Y and Choi, Y S and Wei, Y D and Choo, Y M and Kang, P D and Yoon, H J and Kim, I and Je, Y H and Seo, S J and Lee, S M and Guo, X J and Sohn, H D and Jin, B R}, doi = {10.1186/1471-213X/6/49}, journal-iso = {BMC DEV BIOL}, journal = {BMC DEVELOPMENTAL BIOLOGY}, volume = {6}, unique-id = {22104049}, issn = {1471-213X}, year = {2006} } @article{MTMT:22104050, title = {Dynamic regulation of Drosophila nuclear receptor activity in vivo}, url = {https://m2.mtmt.hu/api/publication/22104050}, author = {Palanker, L and Necakov, A S and Sampson, H M and Ni, R and Hu, C and Thummel, C S and Krause, H M}, doi = {10.1242/dev.02512}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {133}, unique-id = {22104050}, issn = {0950-1991}, year = {2006}, eissn = {1477-9129}, pages = {3549-3562} } @article{MTMT:22192528, title = {Interaction between hormonal signaling pathways in Drosophila melanogaster as revealed by genetic interaction between Methoprene-tolerant and Broad-Complex}, url = {https://m2.mtmt.hu/api/publication/22192528}, author = {Wilson, TG and Yerushalmi, Y and Donnell, DM and Restifo, L}, doi = {10.1534/genetics.105.046631}, journal-iso = {GENETICS}, journal = {GENETICS}, volume = {172}, unique-id = {22192528}, issn = {0016-6731}, year = {2006}, eissn = {1943-2631}, pages = {253-264} } @article{MTMT:22104053, title = {The genomic response to 20-hydroxyecdysone at the onset of Drosophila metamorphosis}, url = {https://m2.mtmt.hu/api/publication/22104053}, author = {Beckstead, RB and Lam, G and Thummel, CS}, doi = {10.1186/gb-2005-6-12-r99}, journal-iso = {GENOME BIOL}, journal = {GENOME BIOLOGY}, volume = {6}, unique-id = {22104053}, issn = {1474-7596}, year = {2005}, eissn = {1474-760X} } @article{MTMT:23438552, title = {Juvenile hormone molecular actions and interactions during development of Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/23438552}, author = {Berger, EM and Dubrovsky, EB}, doi = {10.1016/S0083-6729(05)73006-5}, journal-iso = {VITAM HORM}, journal = {VITAMINS AND HORMONES-ADVANCES IN RESEARCH AND APPLICATIONS}, volume = {73}, unique-id = {23438552}, issn = {0083-6729}, year = {2005}, pages = {175-215} } @article{MTMT:23208875, title = {The steroid hormone-regulated gene Broad Complex is required for dendritic growth of motoneurons during metamorphosis of Drosophila}, url = {https://m2.mtmt.hu/api/publication/23208875}, author = {Consoulas, C and Levine, RB and Restifo, LL}, doi = {10.1002/cne.20499}, journal-iso = {J COMP NEUROL}, journal = {JOURNAL OF COMPARATIVE NEUROLOGY}, volume = {485}, unique-id = {23208875}, issn = {0021-9967}, year = {2005}, eissn = {1096-9861}, pages = {321-337} } @article{MTMT:22104055, title = {Phenotypic analysis of EcR-A mutants suggests that EcR isoforms have unique functions during Drosophila development}, url = {https://m2.mtmt.hu/api/publication/22104055}, author = {Davis, MB and Carney, GE and Robertson, AE and Bender, M}, doi = {10.1016/j.ydbio.2005.03.019}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {282}, unique-id = {22104055}, issn = {0012-1606}, year = {2005}, eissn = {1095-564X}, pages = {385-396} } @article{MTMT:21071323, title = {The ecdysone-induced DHR4 orphan nuclear receptor coordinates growth and maturation in Drosophila}, url = {https://m2.mtmt.hu/api/publication/21071323}, author = {King-Jones, K and Charles, J P and Lam, G and Thummel, C S}, doi = {10.1016/j.cell.2005.03.030}, journal-iso = {CELL}, journal = {CELL}, volume = {121}, unique-id = {21071323}, issn = {0092-8674}, year = {2005}, eissn = {1097-4172}, pages = {773-784} } @article{MTMT:22104058, title = {Ecdysteroid control of metamorphosis in the differentiating adult leg structures of Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/22104058}, author = {Mirth, C}, doi = {10.1016/j.ydbio.2004.10.026}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {278}, unique-id = {22104058}, issn = {0012-1606}, year = {2005}, eissn = {1095-564X}, pages = {163-174} } @article{MTMT:22104056, title = {Ligand-dependent de-repression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing}, url = {https://m2.mtmt.hu/api/publication/22104056}, author = {Schubiger, M and Carre, C and Antoniewski, C and Truman, JW}, doi = {10.1242/dev.02093}, journal-iso = {DEVELOPMENT}, journal = {DEVELOPMENT}, volume = {132}, unique-id = {22104056}, issn = {0950-1991}, year = {2005}, eissn = {1477-9129}, pages = {5239-5248} } @article{MTMT:22104060, title = {The early gene Broad is involved in the ecdysteroid hierarchy governing vitellogenesis of the mosquito Aedes aegypti}, url = {https://m2.mtmt.hu/api/publication/22104060}, author = {Chen, L and Zhu, J and Sun, G and Raikhel, AS}, doi = {10.1677/jme.1.01531}, journal-iso = {J MOL ENDOCRINOL}, journal = {JOURNAL OF MOLECULAR ENDOCRINOLOGY}, volume = {33}, unique-id = {22104060}, issn = {0952-5041}, year = {2004}, eissn = {1479-6813}, pages = {743-761} } @article{MTMT:21291967, title = {Insect basic leucine zipper proteins and their role in cyclic AMP-dependent regulation of gene expression}, url = {https://m2.mtmt.hu/api/publication/21291967}, author = {Poels, J and Vanden, Broeck J}, doi = {10.1016/S0074-7696(04)41005-5}, journal-iso = {INT REV CYTOL}, journal = {INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY}, volume = {241}, unique-id = {21291967}, issn = {0074-7696}, year = {2004}, pages = {277-309} } @article{MTMT:22104063, title = {Hormonal control of a metamorphosis-specific transcriptional factor Broad-Complex in silkworm}, url = {https://m2.mtmt.hu/api/publication/22104063}, author = {Reza, Ams and Kanamori, Y and Shinoda, T and Shimura, S and Mita, K and Nakahara, Y and Kiuchi, M and Kamimura, M}, doi = {10.1016/j.cbpc.2004.09.009}, journal-iso = {COMP BIOCHEM PHYS B}, journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, volume = {139}, unique-id = {22104063}, issn = {1096-4959}, year = {2004}, eissn = {1879-1107}, pages = {753-761} } @article{MTMT:22104059, title = {The molecular site of action of juvenile hormone and juvenile hormone insecticides during metamorphosis: how these compounds kill insects}, url = {https://m2.mtmt.hu/api/publication/22104059}, author = {Wilson, TG}, doi = {10.1016/j.jinsphys.2003.12.004}, journal-iso = {J INSECT PHYSIOL}, journal = {JOURNAL OF INSECT PHYSIOLOGY}, volume = {50}, unique-id = {22104059}, issn = {0022-1910}, year = {2004}, eissn = {1879-1611}, pages = {111-121} } @article{MTMT:22104061, title = {Overexpression of broad: a new insight into its role in the Drosophila prothoracic gland cells}, url = {https://m2.mtmt.hu/api/publication/22104061}, author = {Zhou, XF and Zhou, BH and Truman, JW and Riddiford, LM}, doi = {10.1242/jeb.00855}, journal-iso = {J EXP BIOL}, journal = {JOURNAL OF EXPERIMENTAL BIOLOGY}, volume = {207}, unique-id = {22104061}, issn = {0022-0949}, year = {2004}, eissn = {1477-9145}, pages = {1151-1161} } @article{MTMT:22104070, title = {Evolution of the Drosophila broad locus: the Manduca sexta broad Z4 isoform has biological activity in Drosophila}, url = {https://m2.mtmt.hu/api/publication/22104070}, author = {Bayer, C and Zhou, XF and Zhou, BH and Riddiford, LM and von Kalm, L}, doi = {10.1007/s00427-003-0354-4}, journal-iso = {DEV GENES EVOL}, journal = {DEVELOPMENT GENES AND EVOLUTION}, volume = {213}, unique-id = {22104070}, issn = {0949-944X}, year = {2003}, eissn = {1432-041X}, pages = {471-476} } @article{MTMT:22104069, title = {Genetic interactions between the RhoA and stubble-stubbloid loci suggest a role for a type II transmembrane serine protease in intracellular signaling during Drosophila imaginal disc morphogenesis}, url = {https://m2.mtmt.hu/api/publication/22104069}, author = {Bayer, CA and Halsell, SR and Fristrom, JW and Kiehart, DP and von Kalm, L}, journal-iso = {GENETICS}, journal = {GENETICS}, volume = {165}, unique-id = {22104069}, issn = {0016-6731}, year = {2003}, eissn = {1943-2631}, pages = {1417-1432} } @article{MTMT:22104066, title = {Orphan nuclear receptor beta FTZ-F1 is required for muscle-driven morphogenetic events at the prepupal-pupal transition in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/22104066}, author = {Fortier, TA and Vasa, PP and Woodard, CT}, doi = {10.1016/S0012-1606(03)00036-8}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {257}, unique-id = {22104066}, issn = {0012-1606}, year = {2003}, eissn = {1095-564X}, pages = {153-165} } @article{MTMT:22104065, title = {Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and Broad-Complex gene activity}, url = {https://m2.mtmt.hu/api/publication/22104065}, author = {Sernpere, LF and Sokol, NS and Dubrovsky, EB and Berger, EM and Ambros, V}, doi = {10.1016/S0012-1606(03)00208-2}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {259}, unique-id = {22104065}, issn = {0012-1606}, year = {2003}, eissn = {1095-564X}, pages = {9-18} } @article{MTMT:22104071, title = {E74 exhibits stage-specific hormonal regulation in the epidermis of the tobacco hornworm, Manduca sexta}, url = {https://m2.mtmt.hu/api/publication/22104071}, author = {Stilwell, GE and Nelson, CA and Weller, J and Cui, HY and Hiruma, K and Truman, JW and Riddiford, LM}, doi = {10.1016/S0012-1606(03)00105-2}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {258}, unique-id = {22104071}, issn = {0012-1606}, year = {2003}, eissn = {1095-564X}, pages = {76-90} } @article{MTMT:22104064, title = {Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis}, url = {https://m2.mtmt.hu/api/publication/22104064}, author = {Uhlirova, M and Foy, BD and Beaty, BJ and Olson, KE and Riddiford, LM and Jindra, M}, doi = {10.1073/pnas.2136837100}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {100}, unique-id = {22104064}, issn = {0027-8424}, year = {2003}, eissn = {1091-6490}, pages = {15607-15612} } @article{MTMT:22131160, title = {Genetic modifier screens in Drosophila demonstrate a role for Rho1 signaling in ecdysone-triggered imaginal disc morphogenesis}, url = {https://m2.mtmt.hu/api/publication/22131160}, author = {Ward, RE and Evans, J and Thummel, CS}, journal-iso = {GENETICS}, journal = {GENETICS}, volume = {165}, unique-id = {22131160}, issn = {0016-6731}, year = {2003}, eissn = {1943-2631}, pages = {1397-1415} } @article{MTMT:22104067, title = {GFP in living animals reveals dynamic developmental responses to ecdysone during Drosophila metamorphosis}, url = {https://m2.mtmt.hu/api/publication/22104067}, author = {Ward, RE and Reid, P and Bashirullah, A and D, Avino PP and Thummel, CS}, doi = {10.1016/S0012-1606(02)00100-8}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {256}, unique-id = {22104067}, issn = {0012-1606}, year = {2003}, eissn = {1095-564X}, pages = {389-402} } @article{MTMT:22104073, title = {Loss of the ecdysteroid-inducible E75A orphan nuclear receptor uncouples molting from metamorphosis in Drosophila}, url = {https://m2.mtmt.hu/api/publication/22104073}, author = {Bialecki, M and Shilton, A and Fichtenberg, C and Segraves, WA and Thummel, CS}, doi = {10.1016/S1534-5807(02)00204-6}, journal-iso = {DEV CELL}, journal = {DEVELOPMENTAL CELL}, volume = {3}, unique-id = {22104073}, issn = {1534-5807}, year = {2002}, eissn = {1878-1551}, pages = {209-220} } @article{MTMT:22104079, title = {Control of Dopa decarboxylase gene expression by the Broad- Complex during metamorphosis in Drosophila}, url = {https://m2.mtmt.hu/api/publication/22104079}, author = {Chen, L and Keefe, SL and Hodgetts, RB}, doi = {10.1016/S0925-4773(02)00346-5}, journal-iso = {MECH DEVELOP}, journal = {MECHANISMS OF DEVELOPMENT}, volume = {119}, unique-id = {22104079}, issn = {0925-4773}, year = {2002}, eissn = {1872-6356}, pages = {145-156} } @article{MTMT:22104075, title = {Induction of the early-late Ddc gene during Drosophila metamorphosis by the ecdysone receptor}, url = {https://m2.mtmt.hu/api/publication/22104075}, author = {Chen, L and Reece, C and Keefe, SL and Hawryluk, Gwl and Engstrom, MM and Hodgetts, RB}, doi = {10.1016/S0925-4773(02)00064-3}, journal-iso = {MECH DEVELOP}, journal = {MECHANISMS OF DEVELOPMENT}, volume = {114}, unique-id = {22104075}, issn = {0925-4773}, year = {2002}, eissn = {1872-6356}, pages = {95-107} } @article{MTMT:22104077, title = {Ecdysone triggers the expression of Golgi genes in Drosophila imaginal discs via Broad-complex}, url = {https://m2.mtmt.hu/api/publication/22104077}, author = {Dunne, JC and Kondylis, V and Rabouille, C}, doi = {10.1006/dbio.2002.0632}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {245}, unique-id = {22104077}, issn = {0012-1606}, year = {2002}, eissn = {1095-564X}, pages = {172-186} } @article{MTMT:23406495, title = {Isolation and characterization of novel mutations of the Broad-Complex, a key regulatory gene of ecdysone induction in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/23406495}, author = {Gonzy, G and Pokholkova, GV and Peronnet, F and Mugat, B and Demakova, OV and Kotlikova, IV and Lepesant, JA and Zhimulev, IF}, doi = {10.1016/S0965-1748(01)00097-2}, journal-iso = {INSECT BIOCHEM MOLEC}, journal = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}, volume = {32}, unique-id = {23406495}, issn = {0965-1748}, year = {2002}, eissn = {1879-0240}, pages = {121-132} }