TY  - JOUR
AU  - Szabó, Enikő
AU  - Faragó, Anna
AU  - Bodor, Gergely
AU  - Gémes, Nikolett
AU  - Puskás, László
AU  - Kovács, László
AU  - Szebeni, Gábor
TI  - Identification of immune subsets with distinct lectin binding signatures using multi-parameter flow cytometry: correlations with disease activity in systemic lupus erythematosus
JF  - FRONTIERS IN IMMUNOLOGY
J2  - FRONT IMMUNOL
VL  - 15
PY  - 2024
PG  - 22
SN  - 1664-3224
DO  - 10.3389/fimmu.2024.1380481
UR  - https://m2.mtmt.hu/api/publication/34840255
ID  - 34840255
N1  - Funding Agency and Grant Number: Nemzeti Kutatsi Fejlesztsi s Innovcis Hivatal10.13039/501100011019
 Funding text: No Statement Available
A közlemény a Bolyai János Kutatási Ösztöndíj (BO/00582/22/8) segítségével jött létre.
AB  - Cell surface glycosylation can influence protein-protein interactions with particular relevance to changes in core fucosylation and terminal sialylation. Glycans are ligands for immune regulatory lectin families like galectins (Gals) or sialic acid immunoglobulin-like lectins (Siglecs). This study delves into the glycan alterations within immune subsets of systemic lupus erythematosus (SLE).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Evaluation of binding affinities of Galectin-1, Galectin-3, Siglec-1, <jats:italic>Aleuria aurantia</jats:italic> lectin (AAL, recognizing core fucosylation), and <jats:italic>Sambucus nigra</jats:italic> agglutinin (SNA, specific for α-2,6-sialylation) was conducted on various immune subsets in peripheral blood mononuclear cells (PBMCs) from control and SLE subjects. Lectin binding was measured by multi-parameter flow cytometry in 18 manually gated subsets of T-cells, NK-cells, NKT-cells, B-cells, and monocytes in unstimulated resting state and also after 3-day activation. Stimulated pre-gated populations were subsequently clustered by FlowSOM algorithm based on lectin binding and activation markers, CD25 or HLA-DR.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Elevated AAL, SNA and CD25<jats:sup>+</jats:sup>/CD25<jats:sup>-</jats:sup> SNA binding ratio in certain stimulated SLE T-cell subsets correlated with SLE Disease Activity Index 2000 (SLEDAI-2K) scores. The significantly increased frequencies of activated AAL<jats:sup>low</jats:sup> Siglec-1<jats:sup>low</jats:sup> NK metaclusters in SLE also correlated with SLEDAI-2K indices. In SLE, activated double negative NKTs displayed significantly lower core fucosylation and CD25<jats:sup>+</jats:sup>/CD25<jats:sup>-</jats:sup> Siglec-1 binding ratio, negatively correlating with disease activity. The significantly enhanced AAL binding in resting SLE plasmablasts positively correlated with SLEDAI-2K scores.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Alterations in the glycosylation of immune cells in SLE correlate with disease severity, which might represent potential implications in the pathogenesis of SLE.
LA  - English
DB  - MTMT
ER  -