TY  - JOUR
AU  - Birtalan, Ede
AU  - Dános, Kornél
AU  - Gurbi, Bianka
AU  - Brauswetter, Diána
AU  - Halász, Judit
AU  - Kalocsáné, Piurko V
AU  - Ács, Balázs
AU  - Antal, B
AU  - Mihályi, R
AU  - Pató, Anna Terézia
AU  - Fent, Zoltán
AU  - Polony, Gábor
AU  - Tímár, József
AU  - Tamás, László
TI  - Expression of PD-L1 on Immune Cells Shows Better Prognosis in Laryngeal, Oropharygeal, and Hypopharyngeal Cancer
JF  - APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
J2  - APPL IMMUNOHISTO M M
VL  - 26
PY  - 2018
IS  - 7
SP  - e79
EP  - e85
PG  - 7
SN  - 1541-2016
DO  - 10.1097/PAI.0000000000000590
UR  - https://m2.mtmt.hu/api/publication/3310542
ID  - 3310542
N1  - Cited By :35            
            Export Date: 24 November 2024            
            CODEN: AIMMF
AB  - Despite great enthusiasm towards immunotherapy, reliable biomarkers are still lacking. The importance of subsets based on human papillomavirus (HPV) status is supported by a growing body of evidence. However, role of other possible subgroups such as anatomic localization of primary tumor remains controversial. Our objective was to investigate immune cell infiltrate and checkpoint inhibitor proteins in above-mentioned head and neck cancer subsets. Archival tumor samples of 106 laryngeal, oropharyngeal, and hypopharyngeal cancer patients were stained with PD-L1, PD-L2, PD-1, and CTLA-4 antibodies. Proportion of tumor-infiltrating lymphocytes was assessed as well. In HPV-negative tumors, PD-L1 immune cell positivity was associated with better disease-specific survival. PD-L1 expression on immune cells correlated with improved disease-specific survival in laryngeal tumors. Furthermore, PD-L1 immune cell positivity correlated with CTLA-4 expression on immune cells and it was accompanied by high tumor-infiltrating lymphocyte rate. PD-L1 expression on tumor cells and PD-1 status showed strong correlation in all patients and in oropharyngeal and laryngeal localization, but not in hypopharynx. HPV-negative oropharyngeal cancers showed negative PD-L1 status on tumor cells. CTLA-4 positivity was observed in 49.5% and 20.6% on immune cells and on tumor cells, respectively. We concluded that PD-L1 expression on immune cells indicates better prognosis in laryngeal squamous cell carcinoma and in HPV-negative head and neck squamous cell carcinoma. We have not found any essential differences between anatomic subgroups. A possibly distinct role of hypopharyngeal localization regarding immune activity requires further clarification.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Szentkuti, G
AU  - Dános, Kornél
AU  - Brauswetter, Diána
AU  - Kiszner, Gergő
AU  - Krenács, Tibor
AU  - Csákó, László
AU  - Répássy, Gábor
AU  - Tamás, László
TI  - Correlations Between Prognosis and Regional Biomarker Profiles in Head and Neck Squamous Cell Carcinomas
JF  - PATHOLOGY AND ONCOLOGY RESEARCH
J2  - PATHOL ONCOL RES
VL  - 21
PY  - 2015
IS  - 3
SP  - 643
EP  - 650
PG  - 8
SN  - 1219-4956
DO  - 10.1007/s12253-014-9869-4
UR  - https://m2.mtmt.hu/api/publication/2851040
ID  - 2851040
N1  - Department of Oto-Rhino-Laryngology, Jahn Ferenc South-Pest Hospital, 1st Köves Street, Budapest, 1204, Hungary            
            Department of Oto-Rhino-Laryngology, Head-Neck Surgery, Semmelweis University, Budapest, Hungary            
            MTA-SE Pathobiochemistry Research Group, Budapest, Hungary            
            1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary            
            MTA-SE Tumor Progression Research Group, Budapest, Hungary            
            Cited By :23            
            Export Date: 2 June 2021            
            CODEN: POREF            
            Correspondence Address: Szentkúti, G.; Department of Oto-Rhino-Laryngology, 1st Köves Street, Hungary            
            Chemicals/CAS: epidermal growth factor receptor, 79079-06-4; Autoantigens; Biomarkers, Tumor; collagen type XVII; Cyclin-Dependent Kinase Inhibitor p16; EGFR protein, human; Ki-67 Antigen; Non-Fibrillar Collagens; Receptor, Epidermal Growth Factor; TP53 protein, human; Tumor Suppressor Protein p53
AB  - Head and neck squamous cell carcinomas (HNSCC) show diverse clinicopathological features and are mostly linked with poor outcome. In this study, we tested if the expression of tumor growth, cell cycle and basement membrane anchorage related biomarkers allow prognostic and clinicopathological stratification of HNSCC. Archived HNSCC samples from 226 patients included into tissue microarrays (TMA) were tested using immunohistochemistry. Histopathological evaluation and the analysis of immunostaining for EGFR, Ki67, p53, p16ink4 and Collagen XVII proteins were carried out in digital whole slides. Statistical evaluation was carried out using Pearson's Chi-square test and Kaplan-Meier survival analysis. In the tested cohort, hypopharyngeal cancers had the least favorable, and glottic cancers had the most favorable prognosis. High Ki67 positive tumor cell fractions were associated with significantly worse prognosis and elevated rate of lymph node metastasis. Both Ki67 and EGFR expression correlated significantly with the tumor localization. Ki67 index was the highest in the hypopharyngeal region and it proved to be the lowest in the glottic region. EGFR expression was the highest in the oral cavity and the lowest in the glottic region. The survival rate of patients with p16ink4-negative cancer was significantly lower than of those with p16ink4-positive disease. A significant inverse correlation was found between histological grade and the prognosis of HNSCC. Our data support that elevated Ki67 positive proliferating cell fractions contribute to the unfavorable prognosis of hypopharyngeal cancers, while glottic cancers have the most favorable prognosis because of the lowest Ki67 expression rate.
LA  - English
DB  - MTMT
ER  -