@article{MTMT:3310542,
	title = {Expression of PD-L1 on Immune Cells Shows Better Prognosis in Laryngeal, Oropharygeal, and Hypopharyngeal Cancer},
	url = {https://m2.mtmt.hu/api/publication/3310542},
	author = {Birtalan, Ede and Dános, Kornél and Gurbi, Bianka and Brauswetter, Diána and Halász, Judit and Kalocsáné, Piurko V and Ács, Balázs and Antal, B and Mihályi, R and Pató, Anna Terézia and Fent, Zoltán and Polony, Gábor and Tímár, József and Tamás, László},
	doi = {10.1097/PAI.0000000000000590},
	journal-iso = {APPL IMMUNOHISTO M M},
	journal = {APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY},
	volume = {26},
	unique-id = {3310542},
	issn = {1541-2016},
	abstract = {Despite great enthusiasm towards immunotherapy, reliable biomarkers are still lacking. The importance of subsets based on human papillomavirus (HPV) status is supported by a growing body of evidence. However, role of other possible subgroups such as anatomic localization of primary tumor remains controversial. Our objective was to investigate immune cell infiltrate and checkpoint inhibitor proteins in above-mentioned head and neck cancer subsets. Archival tumor samples of 106 laryngeal, oropharyngeal, and hypopharyngeal cancer patients were stained with PD-L1, PD-L2, PD-1, and CTLA-4 antibodies. Proportion of tumor-infiltrating lymphocytes was assessed as well. In HPV-negative tumors, PD-L1 immune cell positivity was associated with better disease-specific survival. PD-L1 expression on immune cells correlated with improved disease-specific survival in laryngeal tumors. Furthermore, PD-L1 immune cell positivity correlated with CTLA-4 expression on immune cells and it was accompanied by high tumor-infiltrating lymphocyte rate. PD-L1 expression on tumor cells and PD-1 status showed strong correlation in all patients and in oropharyngeal and laryngeal localization, but not in hypopharynx. HPV-negative oropharyngeal cancers showed negative PD-L1 status on tumor cells. CTLA-4 positivity was observed in 49.5% and 20.6% on immune cells and on tumor cells, respectively. We concluded that PD-L1 expression on immune cells indicates better prognosis in laryngeal squamous cell carcinoma and in HPV-negative head and neck squamous cell carcinoma. We have not found any essential differences between anatomic subgroups. A possibly distinct role of hypopharyngeal localization regarding immune activity requires further clarification.},
	year = {2018},
	eissn = {1533-4058},
	pages = {e79-e85},
	orcid-numbers = {Birtalan, Ede/0000-0002-5699-3545; Dános, Kornél/0000-0002-3829-8266; Gurbi, Bianka/0000-0002-5635-6255; Halász, Judit/0000-0001-9834-8619; Ács, Balázs/0000-0002-0972-4633; Pató, Anna Terézia/0000-0003-1238-3662; Fent, Zoltán/0000-0002-3559-0886; Polony, Gábor/0000-0001-5236-8139; Tímár, József/0000-0001-9183-0859; Tamás, László/0000-0003-3723-9149}
}

@article{MTMT:2851040,
	title = {Correlations Between Prognosis and Regional Biomarker Profiles in Head and Neck Squamous Cell Carcinomas},
	url = {https://m2.mtmt.hu/api/publication/2851040},
	author = {Szentkuti, G and Dános, Kornél and Brauswetter, Diána and Kiszner, Gergő and Krenács, Tibor and Csákó, László and Répássy, Gábor and Tamás, László},
	doi = {10.1007/s12253-014-9869-4},
	journal-iso = {PATHOL ONCOL RES},
	journal = {PATHOLOGY AND ONCOLOGY RESEARCH},
	volume = {21},
	unique-id = {2851040},
	issn = {1219-4956},
	abstract = {Head and neck squamous cell carcinomas (HNSCC) show diverse clinicopathological features and are mostly linked with poor outcome. In this study, we tested if the expression of tumor growth, cell cycle and basement membrane anchorage related biomarkers allow prognostic and clinicopathological stratification of HNSCC. Archived HNSCC samples from 226 patients included into tissue microarrays (TMA) were tested using immunohistochemistry. Histopathological evaluation and the analysis of immunostaining for EGFR, Ki67, p53, p16ink4 and Collagen XVII proteins were carried out in digital whole slides. Statistical evaluation was carried out using Pearson's Chi-square test and Kaplan-Meier survival analysis. In the tested cohort, hypopharyngeal cancers had the least favorable, and glottic cancers had the most favorable prognosis. High Ki67 positive tumor cell fractions were associated with significantly worse prognosis and elevated rate of lymph node metastasis. Both Ki67 and EGFR expression correlated significantly with the tumor localization. Ki67 index was the highest in the hypopharyngeal region and it proved to be the lowest in the glottic region. EGFR expression was the highest in the oral cavity and the lowest in the glottic region. The survival rate of patients with p16ink4-negative cancer was significantly lower than of those with p16ink4-positive disease. A significant inverse correlation was found between histological grade and the prognosis of HNSCC. Our data support that elevated Ki67 positive proliferating cell fractions contribute to the unfavorable prognosis of hypopharyngeal cancers, while glottic cancers have the most favorable prognosis because of the lowest Ki67 expression rate.},
	year = {2015},
	eissn = {1532-2807},
	pages = {643-650},
	orcid-numbers = {Dános, Kornél/0000-0002-3829-8266; Kiszner, Gergő/0000-0002-3016-2823; Krenács, Tibor/0000-0001-9164-065X; Répássy, Gábor/0000-0002-9986-7901; Tamás, László/0000-0003-3723-9149}
}