TY - JOUR AU - Augis, Luc AU - Nguyễn, Cảnh Hưng AU - Ciseran, Cécile AU - Wacha, András Ferenc AU - Mercier-Nomé, Françoise AU - Domenichini, Séverine AU - Sizun, Christina AU - Fourmentin, Sophie AU - Legrand, François-Xavier TI - Hydrophobic binary mixtures containing amphotericin B as lipophilic solutions for the treatment of cutaneous leishmaniasis JF - INTERNATIONAL JOURNAL OF PHARMACEUTICS J2 - INT J PHARM VL - 662 PY - 2024 PG - 16 SN - 0378-5173 DO - 10.1016/j.ijpharm.2024.124486 UR - https://m2.mtmt.hu/api/publication/35154279 ID - 35154279 AB - Cutaneous leishmaniasis, caused by Leishmania parasites, requires treatments with fewer side effects than those currently available. The development of a topical solution based on amphotericin B (AmB) was pursued. The considerable interest in deep eutectic solvents (DESs) and their remarkable advantages inspired the search for a suitable hydrophobic excipient. Various mixtures based on commonly used hydrogen bond donors (HBDs) and acceptors (HBAs) for DES preparations were explored. Initial physical and in-vitro screenings showed the potential of quaternary phosphonium salt-based mixtures. Through thermal analysis, it was determined that most of these mixtures did not exhibit eutectic behavior. X-ray scattering studies revealed a sponge-like nanoscale structure. The most promising formulation, based on a combination of trihexyl(tetradecyl)phosphonium chloride and 1-oleoyl-rac-glycerol, showed no deleterious effects through histological evaluation. AmB was fully solubilized at concentrations between 0.5 and 0.8 mg·mL−1, depending on the formulation. The monomeric state of AmB was observed by circular dichroism. In-vitro irritation tests demonstrated acceptable viability for AmB-based formulations up to 0.5 mg·mL−1. Additionally, an ex-vivo penetration study on pig ear skin revealed no transcutaneous passage, confirming AmB retention in healthy, unaffected skin. LA - English DB - MTMT ER - TY - JOUR AU - Hussein, Khabat Noori AU - Friedrich, Laszlo AU - Dalmadi, István AU - Kiskó, Gabriella TI - Evaluation of In Vitro Antimicrobial Activity of Bioactive Compounds and the Effect of Allyl-Isothiocyanate on Chicken Meat Quality under Refrigerated Conditions JF - APPLIED SCIENCES-BASEL J2 - APPL SCI-BASEL VL - 13 PY - 2023 IS - 19 PG - 24 SN - 2076-3417 DO - 10.3390/app131910953 UR - https://m2.mtmt.hu/api/publication/34263680 ID - 34263680 N1 - Department of Food Science and Technology, College of Agricultural Engineering Sciences, University of Duhok, Zakho Street 38, Kurdistan Region, Duhok City, 42001, Iraq Department of Livestock Product and Food Preservation Technology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Ménesi út 43-45, Budapest, 1118, Hungary Department of Food Microbiology, Hygiene, and Safety, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út 14-16, Budapest, 1118, Hungary Export Date: 03 April 2025; Cited By: 6; Correspondence Address: I. Dalmadi; Department of Livestock Product and Food Preservation Technology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Budapest, Ménesi út 43-45, 1118, Hungary; email: dalmadi.istvan@uni-mate.hu AB - Natural antimicrobials are effective against both food-borne pathogens and spoilage bacteria. The current study aimed to evaluate the in vitro antimicrobial activity of eight natural bioactive compounds (BACs) and one synthetic compound, butylated hydroxytoluene (BHT), and to evaluate the effect of one selected BAC (AITC) on the safety and quality of minced chicken meat. Additionally, physicochemical (pH, color, water-holding capacity (WHC), chemical forms of myoglobin (deoxymyoglobin-DeoMb, oxymyoglobin-OxyMb, and metmyoglobin-MetMb), thiobarbituric-acid-reactive substances (TBARs)), and microbiological properties (in vitro antimicrobial activity and determination of minimum inhibitory concentration (MIC)) were also evaluated through electronic-nose odor detection. Allyl-isothiocyanate (AITC), thymol, eugenol, and geraniol showed the broadest spectrum of in vitro antibacterial activity against one major meat spoilage bacterium and five pathogenic bacteria that were tested. Subsequently, AITC was selected to be applied to fresh minced chicken meat at different concentrations (at concentrations of the MIC (MIC-1), two times the MIC (MIC-2), and four times the MIC (MIC-4)). The chicken meat was then vacuum-packaged and kept for up to 14 days at 4 degrees C, and its quality properties were checked during storage. In this study, the addition of low concentrations of AITC (MIC-1) maintained the lightness (L*) and increased the WHC of the meat. High concentrations (MIC-2 and MIC-4) caused a significant increase in lightness (L*) and folded yellowness (b*) value, and they significantly reduced the redness (a*) and TBARS values compared to the control meat. The amounts of MetMb and DeoMb were reduced and the quantity of OxyMb was increased as a result of the addition of AITC to the chicken breast. Throughout storage, particularly at MIC-4, AITC showed the lowest numbers of aerobic mesophilic cells, as well as a reduction in Listeria monocytogenes cell numbers and a decrease in Salmonella Typhimurium counts. In addition, the meat containing MIC-4 did not exhibit growth of Pseudomonas lundensis after 10 days. During the storage period, an electronic-nose assay demonstrated a distinction in the odor buildup of AITC across the various meat groups, and meat treated with MIC-4 showed a trend that was clearly opposite to that of untreated meat. These encouraging results demonstrate the potential of AITC to improve the safety and shelf life of meat and meat products. LA - English DB - MTMT ER - TY - JOUR AU - Plaszkó, Tamás AU - Szűcs, Zsolt AU - Vasas, Gábor AU - Gonda, Sándor TI - Effects of Glucosinolate-Derived Isothiocyanates on Fungi: A Comprehensive Review on Direct Effects, Mechanisms, Structure-Activity Relationship Data and Possible Agricultural Applications JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 7 PY - 2021 IS - 7 SP - 539 SN - 2309-608X DO - 10.3390/jof7070539 UR - https://m2.mtmt.hu/api/publication/32102464 ID - 32102464 N1 - Funding Agency and Grant Number: Hungarian Scientific Research Fund (OTKA)Orszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [128021]; European UnionEuropean Commission; European Social FundEuropean Social Fund (ESF) [EFOP-3.6.1-16-2016-00022] Funding text: The study was supported by the Hungarian Scientific Research Fund (OTKA) 128021 grant which are greatly acknowledged, as well as the European Union and the European Social Fund through projects EFOP-3.6.1-16-2016-00022 "Debrecen Venture Catapult Program". LA - English DB - MTMT ER -