TY  - JOUR
AU  - Pardi, Norbert
AU  - Vámos, Edith
AU  - Újfaludi, Zsuzsanna
AU  - Komonyi, Orbán
AU  - Bodai, László
AU  - Boros, Imre Miklós
TI  - In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53
JF  - ACTA BIOLOGICA HUNGARICA (1983-2018)
J2  - ACTA BIOL HUNG
VL  - 62
PY  - 2011
IS  - 4
SP  - 397
EP  - 412
PG  - 16
SN  - 0236-5383
DO  - 10.1556/ABiol.62.2011.4.6
UR  - https://m2.mtmt.hu/api/publication/1772853
ID  - 1772853
N1  - Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary            
            Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary            
            Cited By :2            
            Export Date: 31 August 2019            
            CODEN: ABHUE            
            Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu            
            Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53
Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary            
            Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary            
            Cited By :2            
            Export Date: 2 September 2019            
            CODEN: ABHUE            
            Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu            
            Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53
Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary            
            Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary            
            Cited By :2            
            Export Date: 12 February 2020            
            CODEN: ABHUE            
            Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu            
            Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53
Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary            
            Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary            
            Cited By :2            
            Export Date: 9 September 2020            
            CODEN: ABHUE            
            Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu            
            Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53
Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary            
            Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary            
            Cited By :2            
            Export Date: 17 January 2021            
            CODEN: ABHUE            
            Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu            
            Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Lukacsovich, T
AU  - Asztalos, Zoltán Imre
AU  - Awano, W
AU  - Baba, K
AU  - Kondo, S
AU  - Niwa, S
AU  - Yamamoto, D
TI  - Dual-tagging gene trap of novel genes in Drosophila melanogaster
JF  - GENETICS
J2  - GENETICS
VL  - 157
PY  - 2001
IS  - 2
SP  - 727
EP  - 742
PG  - 16
SN  - 0016-6731
UR  - https://m2.mtmt.hu/api/publication/1921710
ID  - 1921710
AB  - A gene-trap system is established for Drosophila. Unlike the conventional enhancer-trap system, the gene-trap system allows the recovery only of fly lines whose genes are inactivated by a P-element insertion, i.e., mutants. In the gene-trap system, the reporter gene expression reflects precisely the spatial and temporal expression pattern of the trapped gene. Flies in which gene trap occurred are identified by a two-step screening process using two independent markers, mini-w and Gal4, each indicating the integration of the vector downstream of the promoter of a gene (dual tagging), mini-w has its own promoter but lacks a polyadenylation signal. Therefore, mini-w mRNA is transcribed from its own promoter regardless of the vector integration site in the genome. However, the eyes of flies are not orange or red unless the vector is incorporated into a gene enabling mini-w to be spliced to a downstream exon of the host gene and polyadenylated at the 3' end. The promoter-less Gal4 reporter is expressed as a fusion mRNA only when it is integrated downstream of the promoter of a host gene. The exons of trapped genes can be readily cloned by vectorette RT-PCR, followed by RACE and PCR using cDNA libraries. Thus, the dual-tagging gene-trap system provides a means for (i) efficient mutagenesis, (ii) unequivocal identification of genes responsible for mutant phenotypes, (iii) precise detection of expression patterns of trapped genes, and (iv) rapid cloning of trapped genes.
LA  - English
DB  - MTMT
ER  -