TY - JOUR AU - Molnár, Zsolt AU - Pálföldi, Regina AU - László, Anna AU - Radács, Marianna AU - Sepp, Krisztián AU - Hausinger, Péter AU - Tiszlavicz, László AU - Valkusz, Zsuzsanna AU - Gálfi, Márta TI - Effects of chronic and subtoxic chlorobenzenes on adrenocorticotrophic hormone release JF - JOURNAL OF ENVIRONMENTAL SCIENCES J2 - J ENVIRON SCI (CHINA) VL - 34 PY - 2015 SP - 165 EP - 170 PG - 6 SN - 1001-0742 DO - 10.1016/j.jes.2015.03.014 UR - https://m2.mtmt.hu/api/publication/2934331 ID - 2934331 N1 - Institute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged, H-6725, Hungary Department of Pulmonology, Faculty of Medicine, University of Szeged, Deszk, H-6772, Hungary Department of Medical Physics and Informatics, Faculty of Medicine, University of Szeged, Szeged, H-6725, Hungary Invasive Cardiology Department, Second Department of Internal Medicine and Cardiology, Faculty of Medicine, University of Szeged, Szeged, H-6725, Hungary Endocrine Unit of First Department of Internal Medicine, Faculty of Medicine, University of Szeged, Szeged, H-6725, Hungary Department of Pathology, Faculty of Medicine, University of Szeged, Szeged, H-6725, Hungary Cited By :4 Export Date: 26 May 2022 Correspondence Address: Molnár, Z.; Institute of Applied Science, Hungary; email: molnar.zsolt@jgypk.u-szeged.hu Chemicals/CAS: chlorobenzene, 108-90-7; corticotropin, 11136-52-0, 9002-60-2, 9061-27-2; hexachlorobenzene, 118-74-1, 55600-34-5; 1,2,4-trichlorobenzene; Adrenocorticotropic Hormone; Chlorobenzenes; Environmental Pollutants; Hexachlorobenzene AB - Many environmental chemicals and pesticides have been found to alter neuroendocrine communication in exposed biological objects. The environmental loads have primary and secondary effects that can alter the homeostatic regulation potential. Since it is difficult to avoid human exposition, a potentially important area of research to develop in vivo and in vitro experimental models. In this context, the primary aim of this study was to demonstrate the effects of chlorobenzenes on adrenocorticotrophic hormone (ACTH) release. In our experimental study, male Wistar rats were exposed to 0.1, 1.0 and 10mug/b.w. (body weight)kg of 1,2,4- trichlorobenzene and hexachlorobenzene (ClB) mix via gastric tube for 30, 60 or 90days. At the endpoints of the experiment blood samples were taken and animals were decapitated. Primary, monolayer adenohypophysis cell cultures were prepared by enzymatic and mechanical digestion. The ACTH hormone content in serum and supernatant media was measured by immuno-chemiluminescence assay. The Mg(2+)-dependent ATPase activity was determined by modified method of Martin and Dotty. Significant differences were detected in the hormone release between the control and treated groups. The hormone release was enhanced characteristically in exposed groups depending upon the dose and duration of exposure. The Mg(2+)-ATPase activity enhanced after chronic and subtoxic ClB exposition. Light microscopy revealed that the adenohypophysis seemed to be more abundant. Results indicate that Wistar rats exposed to subtoxic ClB have direct and indirect effects on hypothalamus-hypophysis-adrenal axis. LA - English DB - MTMT ER - TY - JOUR AU - Molnár, Zsolt AU - Pálföldi, Regina AU - László, Anna AU - Radács, Marianna AU - Laszlo, M AU - Hausinger, Péter AU - Tiszlavicz, László AU - Rázga, Zsolt AU - Valkusz, Zsuzsanna AU - Gálfi, Márta TI - The Effects of Hypokalaemia on the Hormone Exocytosis in Adenohypophysis and Prolactinoma Cell Culture Model Systems JF - EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES J2 - EXP CLIN ENDOCR DIAB VL - 122 PY - 2014 IS - 10 SP - 575 EP - 581 PG - 7 SN - 0947-7349 DO - 10.1055/s-0034-1383580 UR - https://m2.mtmt.hu/api/publication/2720015 ID - 2720015 N1 - TÁMOP-4.2.4.A/2-11/1-2012-0001 Nemzeti Kiválóság Program AB - The extracellular ion milieu determines the exocytosis mechanism that is coupled to spontaneous electrical activity. The K+ ion plays crucial role in this mechanism: as the potassium current is associated with membrane hyperpolarization and hormone release through protein cascade activation. The primary aim of this study was to investigate the response mechanisms of normal adenohypophysis and adenohypophyseal prolactinoma cell populations at different extracellular K+ levels with an otherwise isoionic milieu of all other essential ions. We focused on prolactin (PRL) and adrenocorticotrophic hormone (ACTH) release.In our experimental study, female Wistar rats (n=20) were treated with estrone-acetate (150 mug/kg b.w./week) for 6 months to induce prolactinomas in the adenohypophysis. Primary, monolayer cell cultures were prepared by enzymatic and mechanical digestion. PRL and ACTH hormone presence was measured by radioimmunoassay or immuno-chemiluminescence assay. Immunocytochemistry was used to assess the apoptotic cells.Differences between the effects of hypokalaemia on normal adenohypophysis cultures and prolactinoma cell populations were investigated. Significant alteration (p<0.001, n=10) in hormone exocytosis was detected in K+ treated adenohypophyseal and prolactinoma cell cultures compared to untreated groups. Immunocyto-chemistry showed that Bcl-2 expression was reduced under hypokalaemic conditions.The decrease in hormone exocytosis was tightly correlated to the extracellular K+ in both cell types, leading to the conclusion that external K+ may be the major factor for the inhibition of hormone release. The significant increase in hormone content in supernatant media suggests that hypokalaemia may play important role in apoptosis. LA - English DB - MTMT ER - TY - JOUR AU - Wang, RS AU - Saadatpour, A AU - Albert, Réka TI - Boolean modeling in systems biology: an overview of methodology and applications JF - PHYSICAL BIOLOGY J2 - PHYS BIOL VL - 9 PY - 2012 IS - 5 SN - 1478-3967 DO - 10.1088/1478-3975/9/5/055001 UR - https://m2.mtmt.hu/api/publication/2943757 ID - 2943757 LA - English DB - MTMT ER - TY - JOUR AU - Valkusz, Zsuzsanna AU - Nagyéri, György AU - Radács, Marianna AU - Ocskó, Tímea AU - Hausinger, Péter AU - Laszlo, M AU - Laszlo, FA AU - Juhász, Anna AU - Julesz, János AU - Pálföldi, Regina AU - Gálfi, Márta TI - Further analysis of behavioral and endocrine consequences of chronic exposure of male Wistar rats to subtoxic doses of endocrine disruptor chlorobenzenes JF - PHYSIOLOGY & BEHAVIOR J2 - PHYSIOL BEHAV VL - 103 PY - 2011 IS - 5 SP - 421 EP - 430 PG - 10 SN - 0031-9384 DO - 10.1016/j.physbeh.2011.03.016 UR - https://m2.mtmt.hu/api/publication/1791837 ID - 1791837 N1 - Megjegyzés-21497465 WC: Psychology, Biological; Behavioral Sciences Megjegyzés-21808378 WC: Psychology, Biological; Behavioral Sciences Megjegyzés-21808412 WC: Psychology, Biological; Behavioral Sciences Megjegyzés-21953159 : FN Thomson Reuters Web of Knowledge WC: Psychology, Biological; Behavioral Sciences Megjegyzés-21914288 WC: Psychology, Biological; Behavioral Sciences AB - Many chemicals utilized by humans are present as environmental pollutants and may influence homeostasis from neurological, immunological, endocrinological and/or behavioral aspects. Such agents, acting alone or in ambient mixtures, may be biologically active even at extremely low doses, and it may be postulated that stable, bioaccumulative, reactive endorine disruptors may affect central and/or peripheral secretion of arginine-vasopressin (AVP) and oxytocin (OXT) and thereby related physiological and behavioral functions, potentially leading to disorders in exposed subjects. The primary aim of this study was to demonstrate effects of chronic exposure to a low dose of an orally administered chlorobenzene mixture on anxiety-related and aggressive behavior mediated largely by AVP and OXT. Chlorobenzenes were applied to model ambient mixtures of endocrine disruptors. Adult, male Wistar rats were exposed daily to 0.1 mu g/kg of 1,2,4-trichlorobenzene and hexachlorobenzene via a stomach tube for 30,60 or 90 days, after which anxiety-related and aggressive behavioral elements were examined in open-field, elevated plus maze and resident-intruder tests. The plasma levels of AVP, OXT and adrenocorticotrophic hormone at the endpoints were measured by radioimmunoassay or immunochemiluminescence assay. The levels of basal and serotonin- or norepinephrine-stimulated AVP and OXT secretion in pituicyte cultures prepared from the posterior lobe of the pituitaries were also measured. The hormone levels proved to be increased to extents depending on the duration of exposure to the chlorobenzenes. Several anxiety-related and aggressive behavioral elements were also enhanced following chlorobenzene exposure, while certain explorative and locomotive elements of the animals were decreased. As both physiological and behavioral elements were modulated by chronic, subtoxic doses of chlorobenzenes, it is concluded that doses of such environmental pollutants low enough to fall outside the range of legal regulation may pose potential risks of anxiogenic and/or aggressive consequences in exposed subjects, including humans. (C) 2011 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER -