@article{MTMT:2934331, title = {Effects of chronic and subtoxic chlorobenzenes on adrenocorticotrophic hormone release}, url = {https://m2.mtmt.hu/api/publication/2934331}, author = {Molnár, Zsolt and Pálföldi, Regina and László, Anna and Radács, Marianna and Sepp, Krisztián and Hausinger, Péter and Tiszlavicz, László and Valkusz, Zsuzsanna and Gálfi, Márta}, doi = {10.1016/j.jes.2015.03.014}, journal-iso = {J ENVIRON SCI (CHINA)}, journal = {JOURNAL OF ENVIRONMENTAL SCIENCES}, volume = {34}, unique-id = {2934331}, issn = {1001-0742}, abstract = {Many environmental chemicals and pesticides have been found to alter neuroendocrine communication in exposed biological objects. The environmental loads have primary and secondary effects that can alter the homeostatic regulation potential. Since it is difficult to avoid human exposition, a potentially important area of research to develop in vivo and in vitro experimental models. In this context, the primary aim of this study was to demonstrate the effects of chlorobenzenes on adrenocorticotrophic hormone (ACTH) release. In our experimental study, male Wistar rats were exposed to 0.1, 1.0 and 10mug/b.w. (body weight)kg of 1,2,4- trichlorobenzene and hexachlorobenzene (ClB) mix via gastric tube for 30, 60 or 90days. At the endpoints of the experiment blood samples were taken and animals were decapitated. Primary, monolayer adenohypophysis cell cultures were prepared by enzymatic and mechanical digestion. The ACTH hormone content in serum and supernatant media was measured by immuno-chemiluminescence assay. The Mg(2+)-dependent ATPase activity was determined by modified method of Martin and Dotty. Significant differences were detected in the hormone release between the control and treated groups. The hormone release was enhanced characteristically in exposed groups depending upon the dose and duration of exposure. The Mg(2+)-ATPase activity enhanced after chronic and subtoxic ClB exposition. Light microscopy revealed that the adenohypophysis seemed to be more abundant. Results indicate that Wistar rats exposed to subtoxic ClB have direct and indirect effects on hypothalamus-hypophysis-adrenal axis.}, year = {2015}, eissn = {1878-7320}, pages = {165-170}, orcid-numbers = {Molnár, Zsolt/0000-0001-5176-149X; László, Anna/0000-0001-9041-4458; Radács, Marianna/0000-0003-0381-9276; Sepp, Krisztián/0000-0001-9400-5213; Tiszlavicz, László/0000-0003-1134-6587; Valkusz, Zsuzsanna/0000-0003-1928-6160; Gálfi, Márta/0000-0002-7143-133X} } @article{MTMT:2720015, title = {The Effects of Hypokalaemia on the Hormone Exocytosis in Adenohypophysis and Prolactinoma Cell Culture Model Systems}, url = {https://m2.mtmt.hu/api/publication/2720015}, author = {Molnár, Zsolt and Pálföldi, Regina and László, Anna and Radács, Marianna and Laszlo, M and Hausinger, Péter and Tiszlavicz, László and Rázga, Zsolt and Valkusz, Zsuzsanna and Gálfi, Márta}, doi = {10.1055/s-0034-1383580}, journal-iso = {EXP CLIN ENDOCR DIAB}, journal = {EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES}, volume = {122}, unique-id = {2720015}, issn = {0947-7349}, abstract = {The extracellular ion milieu determines the exocytosis mechanism that is coupled to spontaneous electrical activity. The K+ ion plays crucial role in this mechanism: as the potassium current is associated with membrane hyperpolarization and hormone release through protein cascade activation. The primary aim of this study was to investigate the response mechanisms of normal adenohypophysis and adenohypophyseal prolactinoma cell populations at different extracellular K+ levels with an otherwise isoionic milieu of all other essential ions. We focused on prolactin (PRL) and adrenocorticotrophic hormone (ACTH) release.In our experimental study, female Wistar rats (n=20) were treated with estrone-acetate (150 mug/kg b.w./week) for 6 months to induce prolactinomas in the adenohypophysis. Primary, monolayer cell cultures were prepared by enzymatic and mechanical digestion. PRL and ACTH hormone presence was measured by radioimmunoassay or immuno-chemiluminescence assay. Immunocytochemistry was used to assess the apoptotic cells.Differences between the effects of hypokalaemia on normal adenohypophysis cultures and prolactinoma cell populations were investigated. Significant alteration (p<0.001, n=10) in hormone exocytosis was detected in K+ treated adenohypophyseal and prolactinoma cell cultures compared to untreated groups. Immunocyto-chemistry showed that Bcl-2 expression was reduced under hypokalaemic conditions.The decrease in hormone exocytosis was tightly correlated to the extracellular K+ in both cell types, leading to the conclusion that external K+ may be the major factor for the inhibition of hormone release. The significant increase in hormone content in supernatant media suggests that hypokalaemia may play important role in apoptosis.}, year = {2014}, eissn = {1439-3646}, pages = {575-581}, orcid-numbers = {Molnár, Zsolt/0000-0001-5176-149X; László, Anna/0000-0001-9041-4458; Radács, Marianna/0000-0003-0381-9276; Tiszlavicz, László/0000-0003-1134-6587; Rázga, Zsolt/0000-0003-4717-8482; Valkusz, Zsuzsanna/0000-0003-1928-6160; Gálfi, Márta/0000-0002-7143-133X} } @article{MTMT:2943757, title = {Boolean modeling in systems biology: an overview of methodology and applications}, url = {https://m2.mtmt.hu/api/publication/2943757}, author = {Wang, RS and Saadatpour, A and Albert, Réka}, doi = {10.1088/1478-3975/9/5/055001}, journal-iso = {PHYS BIOL}, journal = {PHYSICAL BIOLOGY}, volume = {9}, unique-id = {2943757}, issn = {1478-3967}, year = {2012}, eissn = {1478-3975} } @article{MTMT:1791837, title = {Further analysis of behavioral and endocrine consequences of chronic exposure of male Wistar rats to subtoxic doses of endocrine disruptor chlorobenzenes}, url = {https://m2.mtmt.hu/api/publication/1791837}, author = {Valkusz, Zsuzsanna and Nagyéri, György and Radács, Marianna and Ocskó, Tímea and Hausinger, Péter and Laszlo, M and Laszlo, FA and Juhász, Anna and Julesz, János and Pálföldi, Regina and Gálfi, Márta}, doi = {10.1016/j.physbeh.2011.03.016}, journal-iso = {PHYSIOL BEHAV}, journal = {PHYSIOLOGY & BEHAVIOR}, volume = {103}, unique-id = {1791837}, issn = {0031-9384}, abstract = {Many chemicals utilized by humans are present as environmental pollutants and may influence homeostasis from neurological, immunological, endocrinological and/or behavioral aspects. Such agents, acting alone or in ambient mixtures, may be biologically active even at extremely low doses, and it may be postulated that stable, bioaccumulative, reactive endorine disruptors may affect central and/or peripheral secretion of arginine-vasopressin (AVP) and oxytocin (OXT) and thereby related physiological and behavioral functions, potentially leading to disorders in exposed subjects. The primary aim of this study was to demonstrate effects of chronic exposure to a low dose of an orally administered chlorobenzene mixture on anxiety-related and aggressive behavior mediated largely by AVP and OXT. Chlorobenzenes were applied to model ambient mixtures of endocrine disruptors. Adult, male Wistar rats were exposed daily to 0.1 mu g/kg of 1,2,4-trichlorobenzene and hexachlorobenzene via a stomach tube for 30,60 or 90 days, after which anxiety-related and aggressive behavioral elements were examined in open-field, elevated plus maze and resident-intruder tests. The plasma levels of AVP, OXT and adrenocorticotrophic hormone at the endpoints were measured by radioimmunoassay or immunochemiluminescence assay. The levels of basal and serotonin- or norepinephrine-stimulated AVP and OXT secretion in pituicyte cultures prepared from the posterior lobe of the pituitaries were also measured. The hormone levels proved to be increased to extents depending on the duration of exposure to the chlorobenzenes. Several anxiety-related and aggressive behavioral elements were also enhanced following chlorobenzene exposure, while certain explorative and locomotive elements of the animals were decreased. As both physiological and behavioral elements were modulated by chronic, subtoxic doses of chlorobenzenes, it is concluded that doses of such environmental pollutants low enough to fall outside the range of legal regulation may pose potential risks of anxiogenic and/or aggressive consequences in exposed subjects, including humans. (C) 2011 Elsevier Inc. All rights reserved.}, keywords = {IN-VITRO; MESSENGER-RNA; OXYTOCIN; VASOPRESSIN RELEASE; SUPRAOPTIC NUCLEI; VASOPRESSIN; MONOAMINES; anxiety; NEURAL LOBE; Aggression; RISK-ASSESSMENT; chlorobenzenes; hexachlorobenzene; Oxytocin secretion; HYPOTHALAMIC VASOPRESSIN; NEUROHYPOPHYSEAL TISSUE-CULTURES}, year = {2011}, eissn = {1873-507X}, pages = {421-430}, orcid-numbers = {Valkusz, Zsuzsanna/0000-0003-1928-6160; Radács, Marianna/0000-0003-0381-9276; Gálfi, Márta/0000-0002-7143-133X} }