TY  - JOUR
AU  - Dézsi, László
AU  - Mészáros, Tamás
AU  - Kozma, Gergely Tibor
AU  - H-Velkei, Mária
AU  - Oláh, Csaba Zsolt
AU  - Szabó, Miklós
AU  - Patkó, Zsófia Panna
AU  - Fülöp, Tamás
AU  - Hennies, Mark
AU  - Szebeni, Miklós
AU  - Barta, Bálint András
AU  - Merkely, Béla Péter
AU  - Radovits, Tamás
AU  - Szebeni, János
TI  - A naturally hypersensitive porcine model may help understand the mechanism of COVID-19 mRNA vaccine-induced rare (pseudo) allergic reactions: complement activation as a possible contributing factor
JF  - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE)
J2  - GEROSCIENCE
VL  - 44
PY  - 2022
IS  - 2
SP  - 597
EP  - 618
PG  - 22
SN  - 2509-2715
DO  - 10.1007/s11357-021-00495-y
UR  - https://m2.mtmt.hu/api/publication/32666145
ID  - 32666145
N1  - Tamás Radovits and János Szebeni contributed equally to the article.
AB  - A tiny fraction of people immunized with lipid nanoparticle (LNP)-enclosed mRNA (LNP-mRNA) vaccines develop allergic symptoms following their first or subsequent vaccinations, including anaphylaxis. These reactions resemble complement (C) activation-related pseudoallergy (CARPA) to i.v. administered liposomes, for which pigs provide a naturally oversensitive model. Using this model, we injected i.v. the human vaccination dose (HVD) of BNT162b2 (Comirnaty, CMT) or its 2-fold (2x) or 5-fold (5x) amounts and measured the hemodynamic changes and other parameters of CARPA. We observed in 6 of 14 pigs transient pulmonary hypertension along with thromboxane A2 release into the blood and other hemodynamic and blood cell changes, including hypertension, granulocytosis, lymphopenia, and thrombocytopenia. One pig injected with 5x CMT developed an anaphylactic shock requiring resuscitation, while a repeat dose failed to induce the reaction, implying tachyphylaxis. These typical CARPA symptoms could not be linked to animal age, sex, prior immune stimulation with zymosan, immunization of animals with Comirnaty i.v., or i.m. 2 weeks before the vaccine challenge, and anti-PEG IgM levels in Comirnaty-immunized pigs. Nevertheless, IgM binding to the whole vaccine, used as antigen in an ELISA, was significantly higher in reactive animals compared to non-reactive ones. Incubation of Comirnaty with pig serum in vitro showed significant elevations of C3a anaphylatoxin and sC5b-9, the C-terminal complex. These data raise the possibility that C activation plays a causal or contributing role in the rare HSRs to Comirnaty and other vaccines with similar side effects. Further studies are needed to uncover the factors controlling these vaccine reactions in pigs and to understand their translational value to humans.
LA  - English
DB  - MTMT
ER  -