TY - JOUR AU - Oláh, Imre AU - Felföldi, Balázs AU - Benyeda, Zsófia AU - Kovács, Tamás AU - Nagy, Nándor AU - Magyar, Attila TI - The bursal secretory dendritic cell (BSDC) and the enigmatic chB6+ macrophage-like cell (Mal) JF - POULTRY SCIENCE J2 - POULTRY SCI VL - 101 PY - 2022 IS - 4 PG - 13 SN - 0032-5791 DO - 10.1016/j.psj.2022.101727 UR - https://m2.mtmt.hu/api/publication/32716490 ID - 32716490 N1 - Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, 1094, Hungary Ceva-Phylaxia Ltd, Budapest, 1107, Hungary Biovo Animal Health Ltd., Mohács, 7700, Hungary Export Date: 3 May 2022 Correspondence Address: Oláh, I.; Department of Anatomy, Hungary; email: olah.imre@med.semmelweis-univ.hu Chemicals/CAS: Glycoproteins LA - English DB - MTMT ER - TY - JOUR AU - Felföldi, B. AU - Bódi, Ildikó AU - Herberth-Minkó, Krisztina AU - Benyeda, Z. AU - Nagy, Nándor AU - Magyar, Attila AU - Oláh, Imre TI - Infection of bursal disease virus abrogates the extracellular glycoprotein in the follicular medulla JF - POULTRY SCIENCE J2 - POULTRY SCI VL - 100 PY - 2021 IS - 4 PG - 8 SN - 0032-5791 DO - 10.1016/j.psj.2021.01.023 UR - https://m2.mtmt.hu/api/publication/31921236 ID - 31921236 N1 - Scientific Support and Investigation Unit, Ceva-Phylaxia Co. Ltd., Ceva Animal Health, 1107 Budapest, Hungary, Hungary Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University, Budapest, 1094, Hungary Prophyl Ltd., Hungary, 7700, United States Funding text 1: The authors are grateful for excellent technical assistance to Zsuzsa Vidra and Mária Bakó, and for typing and editing to Melinda Piri. AB - In the medulla of bursal follicle, only the secretory dendritic cell (BSDC) is furnished with secretory machinery. The granular discharge of BSDC appears in membrane-bound and solubilized forms. Movat pentachrome staining proves that the solubilized form is a glycoprotein, which fills up the extracellular space of follicular medulla. The glycoprotein contributes to bursal microenvironment and may be attached to the surface of medullary lymphocytes. The secretory granules of BSDC may be fused, resulting in large, irregular dense bodies, which are the first sign of BSDC transformation to macrophage-like cells (Mal). To determine the effect of infectious bursal disease virus (IBDV) infection on the extracellular glycoprotein and BSDC, SPF chickens were experimentally infected with IBDV. On the surface of BSDC, the secretory substance is in high concentration, which may contribute to primary binding of IBDV to BSDC. The early distribution of IBDV infected cells is in consent with that BSDC. The IBDV infected BSDC rapidly transforms to Mal in which the glycoprotein staining appears. In the dense bodies, the packed virus particles inhibit the virus particles preventing the granular discharge, which may represent the first, early phase of virus replication cycle. The absence of extracellular glycoprotein results in alteration in the medullary microenvironment and subsequently B cell apoptosis. On the surface of medullary B cells, the solubilized secretory substance can be in much lower concentration, which results in secondary binding of IBDV to B cells. In secondary, late phase of virus replication cycle, the virus particles are not packed in electron dense substance which results in cytolytic lymphocytes and presence of virus in extracellular space. The Mal emigrates into the cortex, where induces inflammation, recruiting heterophil granulocyte and monocyte. © 2021 LA - English DB - MTMT ER - TY - JOUR AU - Mató, Tamás AU - Tatár-Kis, Tímea AU - Felföldi, Balázs AU - Jansson, Desirée S. AU - Homonnay, Zalán Gábor AU - Bányai, Krisztián AU - Palya, Vilmos TI - Occurrence and spread of a reassortant very virulent genotype of Infectious Bursal Disease Virus with altered VP2 amino acid profile and pathogenicity in some European countries JF - VETERINARY MICROBIOLOGY J2 - VET MICROBIOL VL - 245 PY - 2020 IS - 6 SN - 0378-1135 DO - 10.1016/j.vetmic.2020.108663 UR - https://m2.mtmt.hu/api/publication/31306819 ID - 31306819 LA - English DB - MTMT ER - TY - JOUR AU - Kabell, S AU - Igyarto, BZ AU - Magyar, Attila AU - Hajdu, Z AU - Biro, E AU - Bisgaard, M AU - Oláh, Imre TI - Impact of heterophil granulocyte depletion caused by 5-fluorouracil on infectious bursal disease virus infection in specific pathogen free chickens JF - AVIAN PATHOLOGY J2 - AVIAN PATHOL VL - 35 PY - 2006 IS - 4 SP - 341 EP - 348 PG - 8 SN - 0307-9457 DO - 10.1080/03079450600821141 UR - https://m2.mtmt.hu/api/publication/1482607 ID - 1482607 N1 - Megjegyzés-21984369 : FN Thomson Reuters Web of Knowledge Z9: 1 Megjegyzés-21984223 Z9: 1 AB - The purpose of this study was to investigate the influence of the cytostatic drug, 5-fluorouracil (5-FU), which causes depletion of heterophil granulocytes, on clinical symptoms and histological lesions during the progress of infectious bursal disease virus (IBDV) infection in chickens. The aim was to disclose the mechanism behind the clinical disease symptoms. Three groups of specific pathogen free chickens were used for the experiment. Chickens in groups 1 and 3 were pretreated with 5-FU, while chickens in group 2 were treated with a placebo. After 5 days, the chickens in groups 2 and 3 were inoculated with the classical IBDV strain F52/70. Bursae of Fabricius were sampled at fixed intervals, and the progress of the infection was monitored by various histological techniques and reverse transcriptase-polymerase chain reaction (RT-PCR). We found correlation between histological observations and RT-PCR results. In the 5-FU pretreated chickens, IBDV caused only mild clinical symptoms, even though histological alterations similar to alterations caused by IBDV were still observed. The 5-FU pretreatment resulted in severe heterophil granulocyte depletion by days 2 and 3 after infection (post inoculation) and increased numbers of bursal secretory dendritic cells in the medulla of the follicles. IBDV infection seemed to induce fusion of secretory dendritic cells, resulting in formation of multinucleated giant cells, loaded with apoptotic B cells and virus particles associated with granules of bursal secretory dendritic cells. Our results indicate that the heterophil granulocytes together with the bursal secretory dendritic cells contribute to the outbreak and/or progress of clinical symptoms. LA - English DB - MTMT ER - TY - JOUR AU - Oláh, Imre AU - Glick, B TI - Dendritic cells in the bursal follicles and germinal centers of the chicken's caecal tonsil express vimentin but not desmin. JF - ANATOMICAL RECORD (1906-2002) J2 - ANAT REC (1906-2002) VL - 243 PY - 1995 IS - 3 SP - 384 EP - 389 PG - 6 SN - 0003-276X DO - 10.1002/ar.1092430313 UR - https://m2.mtmt.hu/api/publication/1482624 ID - 1482624 AB - BACKGROUND: Immunohistochemical studies with anti-vimentin and anti-desmin monoclonal antibodies were designed to determine the origin of bursal secretory dendritic cells (SDC) and follicular dendritic cells. METHODS: The binding sites of anti-vimentin, anti-desmin, and anti-chicken-IgG specific monoclonal antibodies were visualized with a biotinylated anti-mouse-IgG, ABC Elite kit, and 4-chloronaphthol. Cells were double stained (anti-vimentin and rabbit anti-chicken-IgG Fc) to determine if the vimentin positive cells possessed surface IgG. RESULTS: Vimentin positive cells were observed in the cortex and medulla of the bursa and germinal center and lymphoepithelial compartment of the caecal tonsil. The mesenchymal reticular cell, the basic supporting cell of the germinal center, was stained prominently by anti-vimentin and anti-desmin. Both antibodies stained the bursal cortex but only anti-vimentin bound the bursal secretory dendritic cell of the medulla. In addition to being vimentin positive and desmin negative, the bursal secretory dendritic cell possessed and the follicular dendritic cell appeared to possess IgG on their surfaces. In all the observations, B-cells were vimentin negative. CONCLUSION: These studies suggest that follicular dendritic cells and mesenchymal reticular cells in the caecal tonsil's germinal centers may be functionally different cell populations while the bursal secretory dendritic cell and follicular dendritic cell of the caecal tonsil may have a common origin. LA - English DB - MTMT ER - TY - JOUR AU - Oláh, Imre AU - Kendall, C AU - Glick, B TI - Differentiation of bursal secretory-dendritic cells studied with anti-vimentin monoclonal antibody. JF - ANATOMICAL RECORD (1906-2002) J2 - ANAT REC (1906-2002) VL - 233 PY - 1992 IS - 1 SP - 111 EP - 120 PG - 10 SN - 0003-276X DO - 10.1002/ar.1092330115 UR - https://m2.mtmt.hu/api/publication/1483502 ID - 1483502 AB - Embryonic and posthatched differentiation of bursal secretory dendritic cells, which express vimentin intermediate filaments, were studied with anti-vimentin (clone 3B4) and anti-cytokeratin (clone Lu5) monoclonal antibodies. Anti-cytokeratin staining revealed that medullary reticular epithelial cells formed a continuous network at every age, whereas the vimentin positive cells were single and showed dendritic appearance. On the basis of location, number, shape, polarized appearance, and Ia staining, the vimentin-positive cells and secretory dendritic cells appeared to be the same cell. Secretory dendritic cell precursors entered the bursal epithelium between 11 and 13 days of embryogenesis. The first vimentin positive cell appeared in the bud of 14-day embryos. Bud formation preceded the appearance of vimentin-positive cells. These observations suggested that the secretory dendritic cell precursor did not express vimentin when it entered the epithelium. Between 15 days of embryogenesis and 2 weeks of posthatch development, the changes in vimentin staining pattern revealed a cytological differentiation of the vimentin-positive cell. During rapid bursal growth, the number of secretory dendritic cells (vimentin-positive cells) increased about 18 times possibly by proliferation of vimentin-negative precursors in the epithelial arches of the corticomedullary border. LA - English DB - MTMT ER - TY - JOUR AU - Oláh, Imre AU - Glick, B TI - Follicle-associated epithelium and medullary epithelial tissue of the bursa of fabricius are two different compartments. JF - ANATOMICAL RECORD (1906-2002) J2 - ANAT REC (1906-2002) VL - 233 PY - 1992 IS - 4 SP - 577 EP - 587 PG - 11 SN - 0003-276X UR - https://m2.mtmt.hu/api/publication/1483500 ID - 1483500 AB - The bursae of Fabricius from the chicken and turkey were studied by light and electron microscopy and immunohistochemical methods. The study focused on the relationship of follicle-associated epithelium to the medulla. The follicle-associated epithelium was supported by 3 to 5 layers of stratified epithelial cells which were a continuation of the corticomedullary epithelial cells. The follicle-associated epithelium consisted of M cells and scattered secretory dendritic cells. The network of the reticular epithelial cells of the medulla was filled with secretory dendritic cells, B cells, and a few T cells and macrophages. The cellular content of the follicle-associated epithelium and the medulla suggested that they were different cellular compartments. Communication between the follicle associated epithelium and medullary epithelial compartment occurred through the supporting cells of the follicle-associated epithelium. When the supporting layers of the follicle-associated epithelium infolded into the medulla, they formed lamellated epithelial bodies similar to the thymic Hassall bodies. The lamellated bodies enclosed secretory dendritic cells but not lymphocytes. The infolding of supporting cells varied from follicle to follicle. The asynchronization of infolding contributed to heterogeneity of follicle composition. Follicle heterogeneity was demonstrated by differences in reactivity with a battery of monoclonal antibodies. LA - English DB - MTMT ER - TY - JOUR AU - Oláh, Imre AU - Glick, B TI - Bursal secretory cells: an electron microscope study. JF - ANATOMICAL RECORD J2 - ANAT REC VL - 219 PY - 1987 IS - 3 SP - 268 EP - 274 PG - 7 SN - 1932-8486 DO - 10.1002/ar.1092190307 UR - https://m2.mtmt.hu/api/publication/1483512 ID - 1483512 N1 - Megjegyzés-23794826 Megjegyzés-21984459 Z9: 17 Megjegyzés-21993704 : FN Thomson Reuters Web of Knowledge Z9: 17 AB - In addition to lymphocytes, macrophages, and epithelial cells, the bursa medulla possesses a cell we have named the secretory cell. The secretory cell, which makes up approximately 0.5% of the bursal cell population, exhibits an eccentric nucleus with a chromatin pattern similar to that of a small lymphocyte and an elongated cytoplasm with one or more cell processes. The electron-dense cytoplasmic granules of the immature secretory cell are localized around the cytocentrum, while in the mature secretory cell these granules are situated beneath the cell membrane of one process. The granular location endows a polarized appearance to the secretory cell. The surface of the membrane is covered with a finely spotted flocculated substance, which may originate from a granular discharge. The round, ovoid, or irregular-shaped granules reveal a homogeneous or distinctive internal pattern. The cortico-medullary border may be the germinal layer of the bursal medulla. The bursal secretory cell is a modified dendritic cell with possible endocrine functions that may be important in B-cell induction. LA - English DB - MTMT ER -