@article{MTMT:32716490, title = {The bursal secretory dendritic cell (BSDC) and the enigmatic chB6+ macrophage-like cell (Mal)}, url = {https://m2.mtmt.hu/api/publication/32716490}, author = {Oláh, Imre and Felföldi, Balázs and Benyeda, Zsófia and Kovács, Tamás and Nagy, Nándor and Magyar, Attila}, doi = {10.1016/j.psj.2022.101727}, journal-iso = {POULTRY SCI}, journal = {POULTRY SCIENCE}, volume = {101}, unique-id = {32716490}, issn = {0032-5791}, year = {2022}, eissn = {1525-3171}, orcid-numbers = {Kovács, Tamás/0000-0003-4127-4545; Nagy, Nándor/0000-0002-6223-5214} } @article{MTMT:31921236, title = {Infection of bursal disease virus abrogates the extracellular glycoprotein in the follicular medulla}, url = {https://m2.mtmt.hu/api/publication/31921236}, author = {Felföldi, B. and Bódi, Ildikó and Herberth-Minkó, Krisztina and Benyeda, Z. and Nagy, Nándor and Magyar, Attila and Oláh, Imre}, doi = {10.1016/j.psj.2021.01.023}, journal-iso = {POULTRY SCI}, journal = {POULTRY SCIENCE}, volume = {100}, unique-id = {31921236}, issn = {0032-5791}, abstract = {In the medulla of bursal follicle, only the secretory dendritic cell (BSDC) is furnished with secretory machinery. The granular discharge of BSDC appears in membrane-bound and solubilized forms. Movat pentachrome staining proves that the solubilized form is a glycoprotein, which fills up the extracellular space of follicular medulla. The glycoprotein contributes to bursal microenvironment and may be attached to the surface of medullary lymphocytes. The secretory granules of BSDC may be fused, resulting in large, irregular dense bodies, which are the first sign of BSDC transformation to macrophage-like cells (Mal). To determine the effect of infectious bursal disease virus (IBDV) infection on the extracellular glycoprotein and BSDC, SPF chickens were experimentally infected with IBDV. On the surface of BSDC, the secretory substance is in high concentration, which may contribute to primary binding of IBDV to BSDC. The early distribution of IBDV infected cells is in consent with that BSDC. The IBDV infected BSDC rapidly transforms to Mal in which the glycoprotein staining appears. In the dense bodies, the packed virus particles inhibit the virus particles preventing the granular discharge, which may represent the first, early phase of virus replication cycle. The absence of extracellular glycoprotein results in alteration in the medullary microenvironment and subsequently B cell apoptosis. On the surface of medullary B cells, the solubilized secretory substance can be in much lower concentration, which results in secondary binding of IBDV to B cells. In secondary, late phase of virus replication cycle, the virus particles are not packed in electron dense substance which results in cytolytic lymphocytes and presence of virus in extracellular space. The Mal emigrates into the cortex, where induces inflammation, recruiting heterophil granulocyte and monocyte. © 2021}, keywords = {Bursa of Fabricius; bursal secretory dendritic cell transformation; extracellular glycoprotein; infectious bursal disease virus infection; Movat pentachrome staining}, year = {2021}, eissn = {1525-3171}, orcid-numbers = {Nagy, Nándor/0000-0002-6223-5214} } @article{MTMT:31306819, title = {Occurrence and spread of a reassortant very virulent genotype of Infectious Bursal Disease Virus with altered VP2 amino acid profile and pathogenicity in some European countries}, url = {https://m2.mtmt.hu/api/publication/31306819}, author = {Mató, Tamás and Tatár-Kis, Tímea and Felföldi, Balázs and Jansson, Desirée S. and Homonnay, Zalán Gábor and Bányai, Krisztián and Palya, Vilmos}, doi = {10.1016/j.vetmic.2020.108663}, journal-iso = {VET MICROBIOL}, journal = {VETERINARY MICROBIOLOGY}, volume = {245}, unique-id = {31306819}, issn = {0378-1135}, year = {2020}, eissn = {1873-2542}, orcid-numbers = {Homonnay, Zalán Gábor/0000-0003-2473-1775} } @article{MTMT:1482607, title = {Impact of heterophil granulocyte depletion caused by 5-fluorouracil on infectious bursal disease virus infection in specific pathogen free chickens}, url = {https://m2.mtmt.hu/api/publication/1482607}, author = {Kabell, S and Igyarto, BZ and Magyar, Attila and Hajdu, Z and Biro, E and Bisgaard, M and Oláh, Imre}, doi = {10.1080/03079450600821141}, journal-iso = {AVIAN PATHOL}, journal = {AVIAN PATHOLOGY}, volume = {35}, unique-id = {1482607}, issn = {0307-9457}, abstract = {The purpose of this study was to investigate the influence of the cytostatic drug, 5-fluorouracil (5-FU), which causes depletion of heterophil granulocytes, on clinical symptoms and histological lesions during the progress of infectious bursal disease virus (IBDV) infection in chickens. The aim was to disclose the mechanism behind the clinical disease symptoms. Three groups of specific pathogen free chickens were used for the experiment. Chickens in groups 1 and 3 were pretreated with 5-FU, while chickens in group 2 were treated with a placebo. After 5 days, the chickens in groups 2 and 3 were inoculated with the classical IBDV strain F52/70. Bursae of Fabricius were sampled at fixed intervals, and the progress of the infection was monitored by various histological techniques and reverse transcriptase-polymerase chain reaction (RT-PCR). We found correlation between histological observations and RT-PCR results. In the 5-FU pretreated chickens, IBDV caused only mild clinical symptoms, even though histological alterations similar to alterations caused by IBDV were still observed. The 5-FU pretreatment resulted in severe heterophil granulocyte depletion by days 2 and 3 after infection (post inoculation) and increased numbers of bursal secretory dendritic cells in the medulla of the follicles. IBDV infection seemed to induce fusion of secretory dendritic cells, resulting in formation of multinucleated giant cells, loaded with apoptotic B cells and virus particles associated with granules of bursal secretory dendritic cells. Our results indicate that the heterophil granulocytes together with the bursal secretory dendritic cells contribute to the outbreak and/or progress of clinical symptoms.}, keywords = {Animals; Fluorouracil/*pharmacology; Specific Pathogen-Free Organisms; Poultry Diseases/*immunology; *Infectious bursal disease virus; Granulocytes/*drug effects; Birnaviridae Infections/*immunology; Chickens/*immunology}, year = {2006}, eissn = {1465-3338}, pages = {341-348} } @article{MTMT:1482624, title = {Dendritic cells in the bursal follicles and germinal centers of the chicken's caecal tonsil express vimentin but not desmin.}, url = {https://m2.mtmt.hu/api/publication/1482624}, author = {Oláh, Imre and Glick, B}, doi = {10.1002/ar.1092430313}, journal-iso = {ANAT REC (1906-2002)}, journal = {ANATOMICAL RECORD (1906-2002)}, volume = {243}, unique-id = {1482624}, issn = {0003-276X}, abstract = {BACKGROUND: Immunohistochemical studies with anti-vimentin and anti-desmin monoclonal antibodies were designed to determine the origin of bursal secretory dendritic cells (SDC) and follicular dendritic cells. METHODS: The binding sites of anti-vimentin, anti-desmin, and anti-chicken-IgG specific monoclonal antibodies were visualized with a biotinylated anti-mouse-IgG, ABC Elite kit, and 4-chloronaphthol. Cells were double stained (anti-vimentin and rabbit anti-chicken-IgG Fc) to determine if the vimentin positive cells possessed surface IgG. RESULTS: Vimentin positive cells were observed in the cortex and medulla of the bursa and germinal center and lymphoepithelial compartment of the caecal tonsil. The mesenchymal reticular cell, the basic supporting cell of the germinal center, was stained prominently by anti-vimentin and anti-desmin. Both antibodies stained the bursal cortex but only anti-vimentin bound the bursal secretory dendritic cell of the medulla. In addition to being vimentin positive and desmin negative, the bursal secretory dendritic cell possessed and the follicular dendritic cell appeared to possess IgG on their surfaces. In all the observations, B-cells were vimentin negative. CONCLUSION: These studies suggest that follicular dendritic cells and mesenchymal reticular cells in the caecal tonsil's germinal centers may be functionally different cell populations while the bursal secretory dendritic cell and follicular dendritic cell of the caecal tonsil may have a common origin.}, keywords = {Animals; Staining and Labeling/methods; Chickens; Fluorescent Antibody Technique; CYTOSKELETON; cecum; Immunoglobulin G/analysis; Vimentin/*analysis; Lymphoid Tissue/*chemistry/cytology; Germinal Center/*chemistry; Desmin/*analysis; Dendritic Cells/*chemistry; Bursa of Fabricius/anatomy & histology/chemistry/*cytology}, year = {1995}, eissn = {1097-0185}, pages = {384-389} } @article{MTMT:1483502, title = {Differentiation of bursal secretory-dendritic cells studied with anti-vimentin monoclonal antibody.}, url = {https://m2.mtmt.hu/api/publication/1483502}, author = {Oláh, Imre and Kendall, C and Glick, B}, doi = {10.1002/ar.1092330115}, journal-iso = {ANAT REC (1906-2002)}, journal = {ANATOMICAL RECORD (1906-2002)}, volume = {233}, unique-id = {1483502}, issn = {0003-276X}, abstract = {Embryonic and posthatched differentiation of bursal secretory dendritic cells, which express vimentin intermediate filaments, were studied with anti-vimentin (clone 3B4) and anti-cytokeratin (clone Lu5) monoclonal antibodies. Anti-cytokeratin staining revealed that medullary reticular epithelial cells formed a continuous network at every age, whereas the vimentin positive cells were single and showed dendritic appearance. On the basis of location, number, shape, polarized appearance, and Ia staining, the vimentin-positive cells and secretory dendritic cells appeared to be the same cell. Secretory dendritic cell precursors entered the bursal epithelium between 11 and 13 days of embryogenesis. The first vimentin positive cell appeared in the bud of 14-day embryos. Bud formation preceded the appearance of vimentin-positive cells. These observations suggested that the secretory dendritic cell precursor did not express vimentin when it entered the epithelium. Between 15 days of embryogenesis and 2 weeks of posthatch development, the changes in vimentin staining pattern revealed a cytological differentiation of the vimentin-positive cell. During rapid bursal growth, the number of secretory dendritic cells (vimentin-positive cells) increased about 18 times possibly by proliferation of vimentin-negative precursors in the epithelial arches of the corticomedullary border.}, keywords = {Animals; immunohistochemistry; Chickens; Epithelial Cells; Cell Differentiation/physiology; Vimentin/analysis/*immunology; Keratins/analysis/immunology; Intermediate Filaments/chemistry/ultrastructure; Epithelium/physiology/ultrastructure; Dendritic Cells/chemistry/*cytology/physiology; Bursa of Fabricius/chemistry/*cytology/physiology; Antibodies, Monoclonal/*analysis/immunology}, year = {1992}, eissn = {1097-0185}, pages = {111-120} } @article{MTMT:1483500, title = {Follicle-associated epithelium and medullary epithelial tissue of the bursa of fabricius are two different compartments.}, url = {https://m2.mtmt.hu/api/publication/1483500}, author = {Oláh, Imre and Glick, B}, journal-iso = {ANAT REC (1906-2002)}, journal = {ANATOMICAL RECORD (1906-2002)}, volume = {233}, unique-id = {1483500}, issn = {0003-276X}, abstract = {The bursae of Fabricius from the chicken and turkey were studied by light and electron microscopy and immunohistochemical methods. The study focused on the relationship of follicle-associated epithelium to the medulla. The follicle-associated epithelium was supported by 3 to 5 layers of stratified epithelial cells which were a continuation of the corticomedullary epithelial cells. The follicle-associated epithelium consisted of M cells and scattered secretory dendritic cells. The network of the reticular epithelial cells of the medulla was filled with secretory dendritic cells, B cells, and a few T cells and macrophages. The cellular content of the follicle-associated epithelium and the medulla suggested that they were different cellular compartments. Communication between the follicle associated epithelium and medullary epithelial compartment occurred through the supporting cells of the follicle-associated epithelium. When the supporting layers of the follicle-associated epithelium infolded into the medulla, they formed lamellated epithelial bodies similar to the thymic Hassall bodies. The lamellated bodies enclosed secretory dendritic cells but not lymphocytes. The infolding of supporting cells varied from follicle to follicle. The asynchronization of infolding contributed to heterogeneity of follicle composition. Follicle heterogeneity was demonstrated by differences in reactivity with a battery of monoclonal antibodies.}, keywords = {Animals; Microscopy, Electron; Chickens; Epithelial Cells; Antibodies, Monoclonal; Turkeys; T-Lymphocytes/cytology/immunology; B-Lymphocytes/cytology/immunology; Bursa of Fabricius/*anatomy & histology/cytology/immunology}, year = {1992}, eissn = {1097-0185}, pages = {577-587} } @article{MTMT:1483512, title = {Bursal secretory cells: an electron microscope study.}, url = {https://m2.mtmt.hu/api/publication/1483512}, author = {Oláh, Imre and Glick, B}, doi = {10.1002/ar.1092190307}, journal-iso = {ANAT REC}, journal = {ANATOMICAL RECORD}, volume = {219}, unique-id = {1483512}, issn = {1932-8486}, abstract = {In addition to lymphocytes, macrophages, and epithelial cells, the bursa medulla possesses a cell we have named the secretory cell. The secretory cell, which makes up approximately 0.5% of the bursal cell population, exhibits an eccentric nucleus with a chromatin pattern similar to that of a small lymphocyte and an elongated cytoplasm with one or more cell processes. The electron-dense cytoplasmic granules of the immature secretory cell are localized around the cytocentrum, while in the mature secretory cell these granules are situated beneath the cell membrane of one process. The granular location endows a polarized appearance to the secretory cell. The surface of the membrane is covered with a finely spotted flocculated substance, which may originate from a granular discharge. The round, ovoid, or irregular-shaped granules reveal a homogeneous or distinctive internal pattern. The cortico-medullary border may be the germinal layer of the bursal medulla. The bursal secretory cell is a modified dendritic cell with possible endocrine functions that may be important in B-cell induction.}, keywords = {Animals; Microscopy, Electron; Cytoplasmic Granules/ultrastructure; Cytoplasm/ultrastructure; Cell Membrane/ultrastructure; Cell Nucleus/ultrastructure; Chickens/*anatomy & histology; Bursa of Fabricius/*ultrastructure}, year = {1987}, eissn = {1932-8494}, pages = {268-274} }