TY  - JOUR
AU  - Balajthy, András
AU  - Somodi, Sándor
AU  - Pethő, Z
AU  - Péter, Mária
AU  - Varga, Zoltán
AU  - P. Szabó, Gabriella
AU  - Paragh, György
AU  - Vigh, László
AU  - Panyi, György
AU  - Hajdu, Péter Béla
TI  - 7DHC-induced changes of Kv1.3 operation contributes to modified T cell function in Smith-Lemli-Opitz syndrome
JF  - PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
J2  - PFLUG ARCH EUR J PHY
VL  - 468
PY  - 2016
IS  - 8
SP  - 1403
EP  - 1418
PG  - 16
SN  - 0031-6768
DO  - 10.1007/s00424-016-1851-4
UR  - https://m2.mtmt.hu/api/publication/3099238
ID  - 3099238
AB  - In vitro manipulation of membrane sterol level affects the regulation of ion channels and consequently certain cellular functions; however, a comprehensive study that confirms the pathophysiological significance of these results is missing. The malfunction of 7-dehydrocholesterol (7DHC) reductase in Smith-Lemli-Opitz syndrome (SLOS) leads to the elevation of the 7-dehydrocholesterol level in the plasma membrane. T lymphocytes were isolated from SLOS patients to assess the effect of the in vivo altered membrane sterol composition on the operation of the voltage-gated Kv1.3 channel and the ion channel-dependent mitogenic responses. We found that the kinetic and equilibrium parameters of Kv1.3 activation changed in SLOS cells. Identical changes in Kv1.3 operation were observed when control/healthy T cells were loaded with 7DHC. Removal of the putative sterol binding sites on Kv1.3 resulted in a phenotype that was not influenced by the elevation in membrane sterol level. Functional assays exhibited impaired activation and proliferation rate of T cells probably partially due to the modified Kv1.3 operation. We concluded that the altered membrane sterol composition hindered the operation of Kv1.3 as well as the ion channel-controlled T cell functions. © 2016, Springer-Verlag Berlin Heidelberg.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Somodi, Sándor
AU  - Balajthy, András
AU  - Szilágyi, Orsolya
AU  - Pethő, Zoltán Dénes
AU  - Harangi, Mariann
AU  - Paragh, György
AU  - Panyi, György
AU  - Hajdu, Péter Béla
TI  - Analysis of the K+ current in human CD4+ T lymphocytes in hypercholesterolemic state
JF  - CELLULAR IMMUNOLOGY
J2  - CELL IMMUNOL
VL  - 281
PY  - 2013
IS  - 1
SP  - 20
EP  - 26
PG  - 7
SN  - 0008-8749
DO  - 10.1016/j.cellimm.2013.01.004
UR  - https://m2.mtmt.hu/api/publication/2239256
ID  - 2239256
N1  - 162058
AB  - Atherosclerosis involves immune mechanisms: T lymphocytes are found in atherosclerotic plaques, suggesting their activation during atherogenesis. The predominant voltage-gated potassium channel of T cells, Kv1.3 is a key regulator of the Ca2+-dependent activation pathway. In the present experiments we studied the proliferation capacity and functional changes of Kv1.3 channels in T cells from healthy and hypercholestaeremic patients.By means of CFSE-assay (carboxyfluorescein succinimidyl ester) we showed that spontaneous activation rate of lymphocytes in hypercholesterolemia was elevated and the antiCD3/antiCD28 co-stimulation was less effective as compared to the healthy group. Using whole-cell patch-clamping we obtained that the activation and deactivation kinetics of Kv1.3 channels were faster in hypercholesterolemic state but no change in other parameters of Kv1.3 were found (inactivation kinetics, steady-state activation, expression level). We suppose that incorporation of oxLDL species via its raft-rupturing effect can modify proliferative rate of T cells as well as the gating of Kv1.3 channels. © 2013 Elsevier Inc.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Szilágyi, Orsolya
AU  - Boratkó, Anita
AU  - Panyi, György
AU  - Hajdu, Péter Béla
TI  - The role of PSD-95 in the rearrangement of Kv1.3 channels to the immunological synapse
JF  - PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
J2  - PFLUG ARCH EUR J PHY
VL  - 465
PY  - 2013
IS  - 9
SP  - 1341
EP  - 1353
PG  - 13
SN  - 0031-6768
DO  - 10.1007/s00424-013-1256-6
UR  - https://m2.mtmt.hu/api/publication/2273474
ID  - 2273474
N1  - WoS:hiba:000323436300010 2019-03-08 21:58 első szerző nem egyezik
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Tóth, Ágnes
AU  - Szilágyi, Orsolya
AU  - Krasznai, Zoltán
AU  - Panyi, György
AU  - Hajdu, Péter Béla
TI  - Functional consequences of Kv1.3 ion channel rearrangement into the immunological synapse.
JF  - IMMUNOLOGY LETTERS
J2  - IMMUNOL LETT
VL  - 125
PY  - 2009
IS  - 1
SP  - 15
EP  - 21
PG  - 7
SN  - 0165-2478
DO  - 10.1016/j.imlet.2009.05.004
UR  - https://m2.mtmt.hu/api/publication/1321720
ID  - 1321720
N1  - CIN: Immunol Lett. 2009 Aug 15;125(2):156-7. PMID: 19595706
AB  - Formation of immunological synapse (IS), the interface between T cells and antigen presenting cells, is a crucial step in T cell activation. This conjugation formation results in the rearrangement and segregation of a set of membrane bound and cytosolic proteins, including that of the T cell receptor, into membrane domains. It was showed earlier that Kv1.3, the dominant voltage-gated potassium channel of T cells redistributes into the IS on interaction with its specific APC. In the present experiments we investigated the functional consequences of the translocation of Kv1.3 channels into the IS formed between mouse helper T (T(h)2) and B cells. Biophysical characteristics of whole-cell Kv1.3 current in standalone cells (c) or ones in IS (IS) were determined using voltage-clamp configuration of standard whole-cell patch-clamp technique. Patch-clamp recordings showed that the activation of Kv1.3 current slowed (tau(a,IS)=2.36+/-0.13 ms (n=7); tau(a,c)=1.36+/-0.06 ms (n=18)) whereas the inactivation rate increased (tau(i,IS)=263+/-29 ms (n=7); tau(i,c)=365+/-27 ms (n=17)) in cells being in IS compared to the standalone cells. The equilibrium distribution between the open and the closed states of Kv1.3 (voltage-dependence of steady-state activation) was shifted toward the depolarizing potentials in T cells engaged into IS (V(1/2,IS)=-20.9+/-2 mV (n=7), V(1/2,c)=-26.4+/-1.5 mV (n=12)). Thus, segregation of Kv1.3 channels into the IS modifies the gating properties of the channels. Application of protein kinase (PK) inhibitors (PKC: GF109203X, PKA: H89, p56Lck: damnacanthal) demonstrated that increase in the inactivation rate can be explained by the dephosphorylation of the channel protein. However, the slower activation kinetics of Kv1.3 in IS is likely to be the consequence of the redistribution of the channels into distinct membrane domains.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Zsiros, E
AU  - Kis-Tóth, Katalin
AU  - Hajdu, Péter Béla
AU  - Gáspár, Rezső
AU  - Bielanska, J
AU  - Felipe, A
AU  - Rajnavölgyi, Éva
AU  - Panyi, György
TI  - Developmental switch of the expression of ion channels in human dendritic cells.
JF  - JOURNAL OF IMMUNOLOGY
J2  - J IMMUNOL
VL  - 183
PY  - 2009
IS  - 7
SP  - 4483
EP  - 4492
PG  - 10
SN  - 0022-1767
DO  - 10.4049/jimmunol.0803003
UR  - https://m2.mtmt.hu/api/publication/1265715
ID  - 1265715
AB  - Modulation of the expression and activity of plasma membrane ion 
channels is one of the mechanisms by which immune cells can 
regulate their intracellular Ca(2+) signaling pathways required 
for proliferation and/or differentiation. Voltage-gated K+ 
channels, inwardly rectifying K+ channels, and Ca(2+)-activated 
K+ channels have been described to play a major role in 
controlling the membrane potential in lymphocytes and 
professional APCs, such as monocytes, macrophages, and dendritic 
cells (DCs). Our study aimed at the characterization and 
identification of ion channels expressed in the course of human 
DC differentiation from monocytes. We report in this study for 
the first time that immature monocyte-derived DCs express 
voltage-gated Na+ channels in their plasma membrane. The 
analysis of the biophysical and pharmacological properties of 
the current and PCR-based cloning revealed the presence of 
Nav1.7 channels in immature DCs. Transition from the immature to 
a mature differentiation state, however, was accompanied by the 
down-regulation of Nav1.7 expression concomitant with the up-
regulation of voltage-gated Kv1.3 K+ channel expression. The 
presence of Kv1.3 channels seems to be common for immune cells; 
hence, selective Kv1.3 blockers may emerge as candidates for 
inhibiting various functions of mature DCs that involve their 
migratory, cytokine-secreting, and T cell-activating potential.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Panyi, György
AU  - Varga, Zoltán
AU  - Gaspar, R
TI  - Ion channels and lymphocyte activation
JF  - IMMUNOLOGY LETTERS
J2  - IMMUNOL LETT
VL  - 92
PY  - 2004
SP  - 55
EP  - 66
PG  - 12
SN  - 0165-2478
DO  - 10.1016/j.imlet.2003.11.020
UR  - https://m2.mtmt.hu/api/publication/105638
ID  - 105638
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Panyi, György
AU  - Vámosi, György
AU  - Bacsó, Zsolt
AU  - Bagdány, M
AU  - Bodnár, Andrea
AU  - Varga, Zoltán
AU  - Gáspár, Rezső
AU  - Mátyus, László
AU  - Damjanovich, Sándor
TI  - Kv1.3 potassium channels are localized in the immunological synapse formed between cytotoxic and target cells
JF  - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
J2  - P NATL ACAD SCI USA
VL  - 101
PY  - 2004
IS  - 5
SP  - 1285
EP  - 1290
PG  - 6
SN  - 0027-8424
DO  - 10.1073/pnas.0307421100
UR  - https://m2.mtmt.hu/api/publication/1075641
ID  - 1075641
N1  - Panyi G, Vamosi G, Bacso Z are equal first authors
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Hajdu, Péter Béla
AU  - Varga, Zoltán
AU  - Pieri, C
AU  - Panyi, György
AU  - Gáspár, Rezső
TI  - Cholesterol modifies the gating of Kv1.3 in human T lymphocytes
JF  - PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
J2  - PFLUG ARCH EUR J PHY
VL  - 445
PY  - 2003
IS  - 6
SP  - 674
EP  - 682
PG  - 9
SN  - 0031-6768
DO  - 10.1007/s00424-002-0974-y
UR  - https://m2.mtmt.hu/api/publication/105647
ID  - 105647
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Panyi, György
AU  - Bagdány, Miklós
AU  - Bodnár, Andrea
AU  - Vámosi, György
AU  - Szentesi, Gergely
AU  - Jenei, Attila
AU  - Mátyus, László
AU  - Varga, S
AU  - Waldmann, TA
AU  - Gáspár, Rezső
AU  - Damjanovich, Sándor
TI  - Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes
JF  - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
J2  - P NATL ACAD SCI USA
VL  - 100
PY  - 2003
SP  - 2592
EP  - 2597
PG  - 6
SN  - 0027-8424
DO  - 10.1073/pnas.0438057100
UR  - https://m2.mtmt.hu/api/publication/105648
ID  - 105648
AB  - Distribution and lateral organization of Kv1.3 potassium channels and CD3 molecules were studied by using electron microscopy, confocal laser scanning microscopy, and fluorescence resonance energy transfer. Immunogold labeling and electron microscopy showed that the distribution of FLAG epitope-tagged Kv1.3 channels (Kv1.3/FLAG) significantly differs from the stochastic Poisson distribution in the plasma membrane of human T lymphoma cells. Confocal laser scanning microscopy images showed that Kv1.3/FLAG channels and CD3 molecules accumulated in largely overlapping membrane areas. The numerical analysis of crosscorrelation of the spatial intensity distributions yielded a high correlation coefficient (C = 0.64). A different hierarchical level of molecular proximity between Kv1.3/FLAG and CD3 proteins was reported by a high fluorescence resonance energy transfer efficiency (E = 51%). These findings implicate that reciprocal regulation of ion-channel activity, membrane potential, and the function of receptor complexes may contribute to the proper functioning of the immunological synapse.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Seres, Ildikó
AU  - Freyss-Beguin, M
AU  - Mohácsi, Attila
AU  - Kozlovsky, B
AU  - Simon, J
AU  - Devynck, MA
AU  - Fulop, T Jr
TI  - Alteration of lymphocyte membrane phospholipids and intracellular free calcium concentrations in hyperlipidemic subjects.
JF  - ATHEROSCLEROSIS
J2  - ATHEROSCLEROSIS
VL  - 121
PY  - 1996
IS  - 2
SP  - 175
EP  - 183
PG  - 9
SN  - 0021-9150
DO  - 10.1016/0021-9150(95)05714-5
UR  - https://m2.mtmt.hu/api/publication/1863338
ID  - 1863338
N1  - Megjegyzés-26671259
Megjegyzés-22053828
Z9: 8
Megjegyzés-22043980
Z9: 8
AB  - Hypercholesterolemia has been proposed to influence cell functions via changes in membrane composition. The aim of the present study was to determine whether the membrane phospholipid composition of human lymphocytes is modified in hypercholesterolemia and whether these changes are accompanied by functional modifications. The phospholipid fatty acid contents and intracellular free calcium concentrations were determined in peripheral blood lymphocytes from 13 subjects with serum total cholesterol levels ranging from 4.6 to 8.8 mmol/l. The spontaneous basal rate of thymidine incorporation in lymphocyte of hypercholesterolemic individuals increased, while its relative stimulation by ConA was less effective. Important changes in membrane lipid composition, consisting mainly of decrease of the mass of phospholipids, and of associated polyunsaturated fatty acids were observed in hypercholesterolemia. In contrast, the cell cholesterol content was significantly increased. The intracellular free calcium concentration was enhanced and strongly associated with circulating cholesterol levels, cell cholesterol content and phospholipid fatty acids. These results indicate that hypercholesterolemia is accompanied by profound changes in lymphocyte membrane lipid composition and Ca(2+) handling.
LA  - English
DB  - MTMT
ER  -