TY - JOUR AU - Nagyné Naszályi, Lívia AU - Dhaene, E AU - Szigyártó, Imola Csilla AU - Mihály, Judith AU - May, Zoltán AU - Varga, Zoltán AU - Van Driessche, I AU - Martins, JC. AU - Fehér, Krisztina TI - An unsought and expensive way to make gold nanoparticles on the way to the development of SiO2@ZrO2 nanocarriers for cancer vaccination JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 311 PY - 2020 PG - 5 SN - 0167-7322 DO - 10.1016/j.molliq.2020.113307 UR - https://m2.mtmt.hu/api/publication/31312924 ID - 31312924 N1 - Funding Agency and Grant Number: European UnionEuropean Union (EU) [703374]; FWO VlaanderenFWO; Ghent UniversityGhent University; FFEU-ZWAP initiative of the Flemish Government; Hercules grant [AUGE15/011]; Marie Curie Career Integration GrantEuropean Union (EU) [303917]; Research Foundation FlandersFWO [1508414N, 1525517N]; Hungarian Academy of SciencesHungarian Academy of Sciences [BO/004333/18/7]; New National Excellence Program of Debrecen University [UNKP-19-4]; National Research, Development and Innovation Office of Hungary [NKFI/OTKA NN 128368] Funding text: This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703374, FWO Vlaanderen, and Ghent University. The 700 MHz equipment used in this study was funded by the FFEU-ZWAP initiative of the Flemish Government and a Hercules grant (AUGE15/011). K.F. acknowledges the support of the Marie Curie Career Integration Grant (303917 PGN-INNATE) and the Research Grant from the Research Foundation Flanders (1508414N and 1525517N). Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/004333/18/7) and the New National Excellence Program of Debrecen University (UNKP-19-4 Bolyai+). This research was supported by the National Research, Development and Innovation Office of Hungary (grant NKFI/OTKA NN 128368). NMR and Structure Analysis Research Group, Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281 S4, Ghent, B-9000, Belgium Sol-gel Centre for Research on Inorganic Powders and Thin Films Synthesis, Department of Chemistry, Ghent University, Krijgslaan 281 S3, Ghent, B-9000, Belgium Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar Tudósok krt. 2., Budapest, H-1117, Hungary Heidelberg Institute for Theoretical Studies, Schloss-Wolfsbrunnenweg 35, Heidelberg, 69118, Germany Molecular Recognition and Interaction Research Group of the Hungarian Academy of Sciences, University of Debrecen, Egyetem tér 1, Debrecen, H-4032, Hungary Export Date: 2 February 2021 CODEN: JMLID Correspondence Address: Nagy, L.N.; NMR and Structure Analysis Research Group, Krijgslaan 281 S4, Belgium; email: lnaszalyi@gmail.com Funding details: 703374 Funding details: Vlaamse regering, AUGE15/011 Funding details: Marie Curie, 303917 PGN-INNATE Funding details: Universiteit Gent Funding details: National Research, Development and Innovation Office, NKFI/OTKA NN 128368 Funding details: Fonds Wetenschappelijk Onderzoek, FWO Funding details: Fonds Wetenschappelijk Onderzoek, FWO, 1525517N, 1508414N Funding details: Magyar Tudományos Akadémia, MTA, BO/004333/18/7 Funding text 1: This project has received funding from the European Union 's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703374 , FWO Vlaanderen , and Ghent University . The 700 MHz equipment used in this study was funded by the FFEU-ZWAP initiative of the Flemish Government and a Hercules grant ( AUGE15/011 ). K.F. acknowledges the support of the Marie Curie Career Integration Grant ( 303917 PGN-INNATE ) and the Research Grant from the Research Foundation Flanders ( 1508414N and 1525517N ). János Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/004333/18/7) and the New National Excellence Program of Debrecen University (ÚNKP-19-4 Bolyai+). This research was supported by the National Research, Development and Innovation Office of Hungary (grant NKFI/OTKA NN 128368 ). AB - Silica@zirconia core@shell oxide nanoparticles of approx. 50 nm diameter were developed in view of an application as a novel carrier with adjuvant activities for cancer vaccines. To this end, the aim was to cover the surface of the nanocarrier by potent single stranded DNA (ssDNA) type immune stimulators used in cancer vaccination. Prior to characterizing the binding of ssDNA to the nanocarrier surface, the adsorption of deoxynucleoside monophosphate building blocks (dNMPs) was studied. After optimization of conditions for synthesis, solvent exchange and surface modification, the effects and possible interference of several buffers, such as HEPES, PIPES, MOPS and MOPSO, were also investigated. Formation of a new compound was revealed by UV–VIS spectroscopy during the recording of zeta potential vs. pH titration curves in the presence of HEPES and PIPES buffer in the neutral-acidic pH region. We identified this new species as gold ions etched from the electrode of the zeta cuvette, stabilized by the buffers via chelation, which is then followed by their conversion into gold nanoparticles. LA - English DB - MTMT ER - TY - JOUR AU - Pardi, Norbert AU - Hogan, Michael J. AU - Porter, Frederick W. AU - Weissman, Drew TI - mRNA vaccines - a new era in vaccinology JF - NATURE REVIEWS DRUG DISCOVERY J2 - NAT REV DRUG DISCOV VL - 17 PY - 2018 IS - 4 SP - 261 EP - 279 PG - 19 SN - 1474-1776 DO - 10.1038/nrd.2017.243 UR - https://m2.mtmt.hu/api/publication/32045027 ID - 32045027 N1 - Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review AB - mRNA vaccines represent a promising alternative to conventional vaccine approaches because of their high potency, capacity for rapid development and potential for low-cost manufacture and safe administration. However, their application has until recently been restricted by the instability and inefficient in vivo delivery of mRNA. Recent technological advances have now largely overcome these issues, and multiple mRNA vaccine platforms against infectious diseases and several types of cancer have demonstrated encouraging results in both animal models and humans. This Review provides a detailed overview of mRNA vaccines and considers future directions and challenges in advancing this promising vaccine platform to widespread therapeutic use. LA - English DB - MTMT ER - TY - JOUR AU - Nagyné Naszályi, Lívia AU - Polyák, András AU - Mihály, Judith AU - Szécsényi, Á AU - Szigyártó, Imola Csilla AU - Czégény, Zsuzsanna AU - Pekkerné Jakab, Emma AU - Németh, Péter AU - Magda, Balázs AU - Szabó, Pál Tamás AU - Veres, Zsuzsa AU - Jemnitz, Katalin AU - Bertóti, Imre AU - Jóba, Róbert Péter AU - Trencsényi, György AU - Balogh, L AU - Bóta, Attila TI - Silica@zirconia@poly(malic acid) nanoparticle: a promising nanocarrier for theranostic applications JF - JOURNAL OF MATERIALS CHEMISTRY B J2 - J MATER CHEM B VL - 4 ET - 0 PY - 2016 IS - 25 SP - 4420 EP - 4429 PG - 10 SN - 2050-750X DO - 10.1039/c6tb01102k UR - https://m2.mtmt.hu/api/publication/3071427 ID - 3071427 LA - English DB - MTMT ER - TY - JOUR AU - Nagyné Naszályi, Lívia AU - Mihály, Judith AU - Polyák, András AU - Debreczeni, B AU - Csaszar, B AU - Szigyártó, Imola Csilla AU - Wacha, András Ferenc AU - Czégény, Zsuzsanna AU - Pekkerné Jakab, Emma AU - Klébert, Szilvia AU - Drotár, Eszter AU - Dabasi, Gabriella AU - Bóta, Attila AU - Balogh, Lajos AU - Kiss, Éva TI - Inherently fluorescent and porous zirconia colloids: preparation, characterization and drug adsorption studies JF - JOURNAL OF MATERIALS CHEMISTRY B J2 - J MATER CHEM B VL - 3 PY - 2015 IS - 38 SP - 7529 EP - 7537 PG - 9 SN - 2050-750X DO - 10.1039/C5TB00832H UR - https://m2.mtmt.hu/api/publication/2946826 ID - 2946826 AB - Porous, fluorescent zirconia particles of nearly 380 nm diameter were prepared without template molecules or labeling dyes. The porous structure is the result of aggregation-induced particle formation. The inherent fluorescence is assigned to coordinatively unsaturated Zr4+ ions at the sol-gel derived ZrO2 surface. After physico-chemical characterization of the native zirconia particles carboxyl and/or amine bearing drug molecules (d,l-[small alpha]-difluoromethylornithine - DFMO, ursolic acid - UA and doxorubicin - DOX) were adsorbed onto their surface, and the products were analyzed with Fourier-transform infrared spectroscopy (FTIR), thermogravimetry (TG), small-angle X-ray scattering (SAXS), fluorimetry and zeta potential vs. pH measurements. We have found that DOX complexes coordinatively unsaturated Zr4+ ions without dislocating them, while carboxyl-bearing drugs interact with basic surface Zr-OH sites eliminating some of the carbonate species. The adsorption of UA at the zirconia surface shifts considerably the isoelectric point of the surface and thus provides kinetic stability to the particles at physiological pH. An in vivo biodistribution study in two healthy dogs performed by SPECT/CT detection after 99mTc labeling of the nanocarriers has shown the possibility of drug delivery application. LA - English DB - MTMT ER -