TY - JOUR AU - Hegedűs, L. AU - Zámbó, Boglárka AU - Pászty, Katalin AU - Padányi, Rita AU - Varga, Karolina AU - Penniston, J.T. AU - Enyedi, Ágnes TI - Molecular Diversity of Plasma Membrane Ca2+ Transporting ATPases: Their Function Under Normal and Pathological Conditions JF - ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY J2 - ADV EXP MED BIOL VL - 1131 PY - 2020 SP - 93 EP - 129 PG - 37 SN - 0065-2598 DO - 10.1007/978-3-030-12457-1_5 UR - https://m2.mtmt.hu/api/publication/30924863 ID - 30924863 AB - Plasma membrane Ca2+ transport ATPases (PMCA1-4, ATP2B1-4) are responsible for removing excess Ca2+ from the cell in order to keep the cytosolic Ca2+ ion concentration at the low level essential for normal cell function. While these pumps take care of cellular Ca2+ homeostasis they also change the duration and amplitude of the Ca2+ signal and can create Ca2+ gradients across the cell. This is accomplished by generating more than twenty PMCA variants each having the character – fast or slow response, long or short memory, distinct interaction partners and localization signals – that meets the specific needs of the particular cell-type in which they are expressed. It has become apparent that these pumps are essential to normal tissue development and their malfunctioning can be linked to different pathological conditions such as certain types of neurodegenerative and heart diseases, hearing loss and cancer. In this chapter we summarize the complexity of PMCA regulation and function under normal and pathological conditions with particular attention to recent developments of the field. © Springer Nature Switzerland AG 2020. LA - English DB - MTMT ER - TY - JOUR AU - Zámbó, Boglárka AU - Várady, György AU - Padányi, Rita AU - Szabó, Edit Zsuzsanna AU - Németh, Adrienn AU - Langó, Tamás AU - Enyedi, Ágnes AU - Sarkadi, Balázs TI - Decreased calcium pump expression in human erythrocytes is connected to a minor haplotype in the ATP2B4 gene JF - CELL CALCIUM J2 - CELL CALCIUM VL - 65 PY - 2017 SP - 73 EP - 79 PG - 7 SN - 0143-4160 DO - 10.1016/j.ceca.2017.02.001 UR - https://m2.mtmt.hu/api/publication/3190054 ID - 3190054 N1 - Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudosok krt. 2, Budapest, 1117, Hungary 2nd Department of Pathology, Faculty of Medicine, Semmelweis University, Ulloi ut 26., Budapest, 1085, Hungary Molecular Oncology Research Group of the Hungarian Academy of Sciences and Semmelweis University, Ulloi ut 26., Budapest, 1085, Hungary MTA-SE Molecular Biophysics Research Group, Department of Biophysics and Radiation Biology, Semmelweis University, Tuzolto u. 37-43., Budapest, 1094, Hungary Cited By :22 Export Date: 10 February 2024 CODEN: CECAD Correspondence Address: Sarkadi, B.; Institute of Enzymology, Magyar Tudosok krt. 2, Hungary; email: sarkadi@biomembrane.hu Chemicals/CAS: adenosine triphosphatase (calcium); hemoglobin, 9008-02-0; ATP2B4 protein, human; Hemoglobins; Plasma Membrane Calcium-Transporting ATPases AB - Plasma membrane Ca2+-ATPases are key calcium exporter proteins in most tissues, and PMCA4b is the main calcium transporter in the human red blood cells (RBCs). In order to assess the expression level of PMCA4b, we have developed a flow cytometry and specific antibody binding method to quantitatively detect this protein in the erythrocyte membrane. Interestingly, we found several healthy volunteers showing significantly reduced expression of RBC-PMCA4b. Western blot analysis of isolated RBC membranes confirmed this observation, and indicated that there are no compensatory alterations in other PMCA isoforms. In addition, reduced PMCA4b levels correlated with a lower calcium extrusion capacity in these erythrocytes. When exploring the potential genetic background of the reduced PMCA4b levels, we found no missense mutations in the ATP2B4 coding regions, while a formerly unrecognized minor haplotype in the predicted second promoter region closely correlated with lower erythrocyte PMCA4b protein levels. In recent GWA studies, SNPs in this ATP2B4 haplotype have been linked to reduced mean corpuscular hemoglobin concentrations (MCHC), and to protection against malaria infection. Our data suggest that an altered regulation of gene expression is responsible for the reduced RBC-PMCA4b levels that is probably linked to the development of human disease-related phenotypes. LA - English DB - MTMT ER - TY - JOUR AU - Padányi, Rita AU - Pászty, Katalin AU - Hegedűs, Luca AU - Varga, Karolina AU - Béla, Papp AU - John, T Penniston AU - Enyedi, Ágnes TI - Multifaceted plasma membrane Ca2+ pumps: From structure to intracellular Ca2+ handling and cancer JF - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH J2 - BBA-MOL CELL RES VL - 1863 PY - 2016 IS - 6 SP - 1351 EP - 1363 PG - 13 SN - 0167-4889 DO - 10.1016/j.bbamcr.2015.12.011 UR - https://m2.mtmt.hu/api/publication/3015469 ID - 3015469 N1 - The first two authors contributed equally to this work. (Megosztott elsőszerzők) LA - English DB - MTMT ER -