TY - JOUR AU - Pál, Ildikó AU - Nyitrai, Gabriella AU - Kardos, Julianna AU - Héja, László TI - Neuronal and Astroglial Correlates Underlying Spatiotemporal Intrinsic Optical Signal in the Rat Hippocampal Slice JF - PLOS ONE J2 - PLOS ONE VL - 8 PY - 2013 IS - 3 SN - 1932-6203 DO - 10.1371/journal.pone.0057694 UR - https://m2.mtmt.hu/api/publication/2222611 ID - 2222611 N1 - Published: March 01, 2013 Funding Agency and Grant Number: [ERA-Chemistry OTKA 102166]; [TECH-09-AI-2009-0117 NKFP NANOSEN9] Funding text: This work was supported by grants ERA-Chemistry OTKA 102166 (http://www.otka.hu/) and TECH-09-AI-2009-0117 NKFP NANOSEN9 (http://www.nih.gov.hu/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Cited By :22 Export Date: 6 April 2021 Correspondence Address: Pál, I.; Department of Functional Pharmacology, , Budapest, Hungary; email: pal.ildiko@ttk.mta.hu LA - English DB - MTMT ER - TY - JOUR AU - Devor, A AU - Ulbert, István AU - Dunn, AK AU - Narayanan, SN AU - Jones, SR AU - Andermann, ML AU - Boas, DA AU - Dale, AM TI - Coupling of the cortical hemodynamic response to cortical and thalamic neuronal activity. JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 102 PY - 2005 IS - 10 SP - 3822 EP - 3827 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.0407789102 UR - https://m2.mtmt.hu/api/publication/1121150 ID - 1121150 N1 - Funding Agency and Grant Number: NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR014075] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB000790] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS044623] Funding Source: NIH RePORTER; NCRR NIH HHS [P41 RR 14075, P41 RR014075] Funding Source: Medline; NIBIB NIH HHS [R01 EB 00790, R01 EB000790] Funding Source: Medline; NINDS NIH HHS [NS 44623, R01 NS044623] Funding Source: Medline AB - Accurate interpretation of functional MRI (fMRI) signals requires knowledge of the relationship between the hemodynamic response and the neuronal activity that underlies it. Here we address the question of coupling between pre- and postsynaptic neuronal activity and the hemodynamic response in rodent somatosensory (Barrel) cortex in response to single-whisker deflection. Using full-field multiwavelength optical imaging of hemoglobin oxygenation and electrophysiological recordings of spiking activity and local field potentials, we demonstrate that a point hemodynamic measure is influenced by neuronal activity across multiple cortical columns. We demonstrate that the hemodynamic response is a spatiotemporal convolution of the neuronal activation. Therefore, positive hemodynamic response in one cortical column might be explained by neuronal activity not only in that column but also in the neighboring columns. Thus, attempts at characterizing the neurovascular relationship based on point measurements of electrophysiology and hemodynamics may yield inconsistent results, depending on the spatial extent of neuronal activation. The finding that the hemodynamic signal observed at a given location is a function of electrophysiological activity over a broad spatial region helps explain a previously observed increase of local vascular response beyond the saturation of local neuronal activity. We also demonstrate that the oxy- and total-hemoglobin hemodynamic responses can be well approximated by space-time separable functions with an antagonistic center-surround spatial pattern extending over several millimeters. The surround "negative" hemodynamic activity did not correspond to observable changes in neuronal activity. The complex spatial integration of the hemodynamic response should be considered when interpreting fMRI data. LA - English DB - MTMT ER - TY - JOUR AU - Devor, A AU - Dunn, AK AU - Andermann, ML AU - Ulbert, István AU - Boas, DA AU - Dale, AM TI - Coupling of total hemoglobin concentration, oxygenation, and neural activity in rat somatosensory cortex. JF - NEURON J2 - NEURON VL - 39 PY - 2003 IS - 2 SP - 353 EP - 359 PG - 7 SN - 0896-6273 DO - 10.1016/S0896-6273(03)00403-3 UR - https://m2.mtmt.hu/api/publication/1121144 ID - 1121144 N1 - Funding Agency and Grant Number: NATIONAL CENTER FOR RESEARCH RESOURCES [R01RR013609, P41RR014075] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB000790] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS044623] Funding Source: NIH RePORTER; NCRR NIH HHS [R01 RR13609, P41 RR14075] Funding Source: Medline; NIBIB NIH HHS [R01 EB00790-01A2, R01 EB000790] Funding Source: Medline; NINDS NIH HHS [R01 NS044623] Funding Source: Medline AB - Recent advances in brain imaging techniques, including functional magnetic resonance imaging (fMRI), offer great promise for noninvasive mapping of brain function. However, the indirect nature of the imaging signals to the underlying neural activity limits the interpretation of the resulting maps. The present report represents the first systematic study with sufficient statistical power to quantitatively characterize the relationship between changes in blood oxygen content and the neural spiking and synaptic activity. Using two-dimensional optical measurements of hemodynamic signals, simultaneous recordings of neural activity, and an event-related stimulus paradigm, we demonstrate that (1) there is a strongly nonlinear relationship between electrophysiological measures of neuronal activity and the hemodynamic response, (2) the hemodynamic response continues to grow beyond the saturation of electrical activity, and (3) the initial increase in deoxyhemoglobin that precedes an increase in blood volume is counterbalanced by an equal initial decrease in oxyhemoglobin. LA - English DB - MTMT ER -