TY - JOUR AU - Hollandi, Réka AU - Diósdi, Ákos AU - Hollandi, Gábor AU - Moshkov, Nikita AU - Horváth, Péter TI - AnnotatorJ: an ImageJ plugin to ease hand annotation of cellular compartments JF - MOLECULAR BIOLOGY OF THE CELL J2 - MOL BIOL CELL VL - 31 PY - 2020 IS - 20 SP - 2179 EP - 2186 PG - 8 SN - 1059-1524 DO - 10.1091/mbc.E20-02-0156 UR - https://m2.mtmt.hu/api/publication/31612924 ID - 31612924 N1 - Cited By :13 Export Date: 12 July 2022 CODEN: MBCEE Correspondence Address: Horváth, P.; Synthetic and Systems Biology Unit, Hungary; email: horvath.peter@brc.hu AB - AnnotatorJ combines single-cell identification with deep learning (DL) and manual annotation. Cellular analysis quality depends on accurate and reliable detection and segmentation of cells so that the subsequent steps of analyses, for example, expression measurements, may be carried out precisely and without bias. DL has recently become a popular way of segmenting cells, performing unimaginably better than conventional methods. However, such DL applications may be trained on a large amount of annotated data to be able to match the highest expectations. High-quality annotations are unfortunately expensive as they require field experts to create them, and often cannot be shared outside the lab due to medical regulations. We propose AnnotatorJ, an ImageJ plugin for the semiautomatic annotation of cells (or generally, objects of interest) on (not only) microscopy images in 2D that helps find the true contour of individual objects by applying U-Net-based presegmentation. The manual labor of hand annotating cells can be significantly accelerated by using our tool. Thus, it enables users to create such datasets that could potentially increase the accuracy of state-of-the-art solutions, DL or otherwise, when used as training data. LA - English DB - MTMT ER - TY - JOUR AU - Dukay, Brigitta AU - Csoboz, Bálint AU - Tóth, Erzsébet Melinda TI - Heat-shock proteins in neuroinflammation JF - FRONTIERS IN PHARMACOLOGY J2 - FRONT PHARMACOL VL - 10 PY - 2019 SN - 1663-9812 DO - 10.3389/fphar.2019.00920 UR - https://m2.mtmt.hu/api/publication/30784321 ID - 30784321 N1 - Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Export Date: 24 September 2019 Correspondence Address: Tóth, M.E.; Institute of Biochemistry, Biological Research Centre, Hungarian Academy of SciencesHungary; email: toth.erzsebetmelinda@brc.mta.hu Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Export Date: 26 September 2019 Correspondence Address: Tóth, M.E.; Institute of Biochemistry, Biological Research Centre, Hungarian Academy of SciencesHungary; email: toth.erzsebetmelinda@brc.mta.hu Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Export Date: 27 September 2019 Correspondence Address: Tóth, M.E.; Institute of Biochemistry, Biological Research Centre, Hungarian Academy of SciencesHungary; email: toth.erzsebetmelinda@brc.mta.hu Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Cited By :1 Export Date: 14 February 2020 Correspondence Address: Tóth, M.E.; Institute of Biochemistry, Biological Research Centre, Hungarian Academy of SciencesHungary; email: toth.erzsebetmelinda@brc.mta.hu LA - English DB - MTMT ER - TY - JOUR AU - Harazin, András AU - Bocsik, Alexandra AU - Barna, Lilla AU - Kincses, András AU - Váradi, Judit AU - Fenyvesi, Ferenc AU - Tubak, Vilmos AU - Deli, Mária Anna AU - Vecsernyés, Miklós TI - Protection of cultured brain endothelial cells from cytokine-induced damage by α-melanocyte stimulating hormone JF - PEERJ J2 - PEERJ VL - 6 PY - 2018 PG - 22 SN - 2167-8359 DO - 10.7717/peerj.4774 UR - https://m2.mtmt.hu/api/publication/3371756 ID - 3371756 N1 - These authors have contributed equally to this work: András Harazin​, Alexandra Bocsik​. LA - English DB - MTMT ER - TY - JOUR AU - Penke, Botond AU - Bogár, Ferenc AU - Crul, Tim AU - Sántha, Miklós AU - Tóth, Erzsébet Melinda AU - Vigh, László TI - Heat shock proteins and autophagy pathways in neuroprotection: From molecular bases to pharmacological interventions JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 19 PY - 2018 IS - 1 SN - 1661-6596 DO - 10.3390/ijms19010325 UR - https://m2.mtmt.hu/api/publication/3326952 ID - 3326952 N1 - N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27148595 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27148656 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186329 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186332 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186337 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186339 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186346 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186352 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186360 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186363 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186365 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186366 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186368 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186391 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186392 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186393 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186398 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186407 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186408 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186410 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186411 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186413 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186414 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186415 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186416 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186418 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186420 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186423 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186428 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186431 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186442 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186443 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27186445 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27201759 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27201904 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27202296 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27210921 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. Megjegyzés-27211166 N1 Funding details: GINOP-2.2.1-15-2016-00007, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding details: GINOP-2.3.2-15-2016-00034, STRIDE Center, Southeastern Transportation Research, Innovation, Development and Education Center N1 Funding text: Acknowledgments: This work was supported by the Hungarian National Research, Development and Innovation Office NKFIH research grants GINOP-2.3.2-15-2016-00034 and GINOP-2.2.1-15-2016-00007. AB - Neurodegenerative diseases (NDDs) such as Alzheimer’s disease, Parkinson’s disease and Huntington’s disease (HD), amyotrophic lateral sclerosis, and prion diseases are all characterized by the accumulation of protein aggregates (amyloids) into inclusions and/or plaques. The ubiquitous presence of amyloids in NDDs suggests the involvement of disturbed protein homeostasis (proteostasis) in the underlying pathomechanisms. This review summarizes specific mechanisms that maintain proteostasis, including molecular chaperons, the ubiquitin-proteasome system (UPS), endoplasmic reticulum associated degradation (ERAD), and different autophagic pathways (chaperon mediated-, micro-, and macro-autophagy). The role of heat shock proteins (Hsps) in cellular quality control and degradation of pathogenic proteins is reviewed. Finally, putative therapeutic strategies for efficient removal of cytotoxic proteins from neurons and design of new therapeutic targets against the progression of NDDs are discussed. © 2018 by the authors. LA - English DB - MTMT ER - TY - JOUR AU - Szalay, Gergely AU - Martinecz, Bernadett AU - Lénárt, Nikolett AU - Környei, Zsuzsanna AU - Orsolits, Barbara AU - Judák, Linda AU - Császár, Eszter AU - Fekete, Rebeka AU - West, BL AU - Katona, Gergely AU - Rózsa J., Balázs AU - Dénes, Ádám TI - Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 7 PY - 2016 PG - 13 SN - 2041-1723 DO - 10.1038/ncomms11499 UR - https://m2.mtmt.hu/api/publication/3060238 ID - 3060238 N1 - These authors contributed equally to this work: Gergely Szalay, Bernadett Martinecz, Nikolett Lenart These authors jointly supervised this work: Balazs Rozsa, Adam Denes Funding details: KTIA-13-NAP-A-I/2 Funding details: Horizon 2020 Framework Programme, H2020 Funding details: Seventh Framework Programme, FP7, 323945 Funding details: European Research Council, ERC, 682426, KTIA-NAP-13-2-2015-0006, SH/7/2/8 Funding details: Hungarian Scientific Research Fund, OTKA, K109743 Funding details: Magyar Tudományos Akadémia, MTA, FP7-ICT-2011-C 323945, K105997, T?T-10-1-2011-0389, VKSZ-14-1-2015-0155 Funding text 1: Funding was provided by OTKA K109743 (A.D.) and the Hungarian Brain Research Program KTIA-13-NAP-A-I/2 (A.D.). A.D. is supported by the Bolyai Janos Research Scholarship from the Hungarian Academy of Sciences. The authors thank the Hungarian-French grant (T?T-10-1-2011-0389), OTKA (K105997), FP7-ICT-2011-C 323945 (3 ? 3Dimaging), VKSZ-14-1-2015-0155, ERC 682426, KTIA-NAP-13-2-2015-0006 and Swiss-Hungarian grant (SH/7/2/8) for additional support. We thank the Cell Biology Center (Flow Cytometry Core Facility) at the Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest, Hungary for their assistance and Csaba D?vid for his help in the development of macros used for analysis of microglia process coverage. We thank L?szl? Barna, Vivien Micz?n and the NMC Imaging Center at the Institute of Experimental Medicine for kindly providing microscopy support. Funding details: KTIA-13-NAP-A-I/2 Funding details: Horizon 2020 Framework Programme, H2020 Funding details: Seventh Framework Programme, FP7, 323945 Funding details: European Research Council, ERC, 682426, KTIA-NAP-13-2-2015-0006, SH/7/2/8 Funding details: Hungarian Scientific Research Fund, OTKA, K109743 Funding details: Magyar Tudományos Akadémia, MTA, FP7-ICT-2011-C 323945, K105997, T?T-10-1-2011-0389, VKSZ-14-1-2015-0155 Funding text 1: Funding was provided by OTKA K109743 (A.D.) and the Hungarian Brain Research Program KTIA-13-NAP-A-I/2 (A.D.). A.D. is supported by the Bolyai Janos Research Scholarship from the Hungarian Academy of Sciences. The authors thank the Hungarian-French grant (T?T-10-1-2011-0389), OTKA (K105997), FP7-ICT-2011-C 323945 (3 ? 3Dimaging), VKSZ-14-1-2015-0155, ERC 682426, KTIA-NAP-13-2-2015-0006 and Swiss-Hungarian grant (SH/7/2/8) for additional support. We thank the Cell Biology Center (Flow Cytometry Core Facility) at the Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest, Hungary for their assistance and Csaba D?vid for his help in the development of macros used for analysis of microglia process coverage. We thank L?szl? Barna, Vivien Micz?n and the NMC Imaging Center at the Institute of Experimental Medicine for kindly providing microscopy support. Funding details: KTIA-13-NAP-A-I/2 Funding details: Horizon 2020 Framework Programme, H2020 Funding details: Seventh Framework Programme, FP7, 323945 Funding details: European Research Council, ERC, 682426, KTIA-NAP-13-2-2015-0006, SH/7/2/8 Funding details: Hungarian Scientific Research Fund, OTKA, K109743 Funding details: Magyar Tudományos Akadémia, MTA, FP7-ICT-2011-C 323945, K105997, T?T-10-1-2011-0389, VKSZ-14-1-2015-0155 Funding text 1: Funding was provided by OTKA K109743 (A.D.) and the Hungarian Brain Research Program KTIA-13-NAP-A-I/2 (A.D.). A.D. is supported by the Bolyai Janos Research Scholarship from the Hungarian Academy of Sciences. The authors thank the Hungarian-French grant (T?T-10-1-2011-0389), OTKA (K105997), FP7-ICT-2011-C 323945 (3 ? 3Dimaging), VKSZ-14-1-2015-0155, ERC 682426, KTIA-NAP-13-2-2015-0006 and Swiss-Hungarian grant (SH/7/2/8) for additional support. We thank the Cell Biology Center (Flow Cytometry Core Facility) at the Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest, Hungary for their assistance and Csaba D?vid for his help in the development of macros used for analysis of microglia process coverage. We thank L?szl? Barna, Vivien Micz?n and the NMC Imaging Center at the Institute of Experimental Medicine for kindly providing microscopy support. Funding details: KTIA-13-NAP-A-I/2 Funding details: Horizon 2020 Framework Programme, H2020 Funding details: Seventh Framework Programme, FP7, 323945 Funding details: European Research Council, ERC, 682426, KTIA-NAP-13-2-2015-0006, SH/7/2/8 Funding details: Hungarian Scientific Research Fund, OTKA, K109743 Funding details: Magyar Tudományos Akadémia, MTA, FP7-ICT-2011-C 323945, K105997, T?T-10-1-2011-0389, VKSZ-14-1-2015-0155 Funding text 1: Funding was provided by OTKA K109743 (A.D.) and the Hungarian Brain Research Program KTIA-13-NAP-A-I/2 (A.D.). A.D. is supported by the Bolyai Janos Research Scholarship from the Hungarian Academy of Sciences. The authors thank the Hungarian-French grant (T?T-10-1-2011-0389), OTKA (K105997), FP7-ICT-2011-C 323945 (3 ? 3Dimaging), VKSZ-14-1-2015-0155, ERC 682426, KTIA-NAP-13-2-2015-0006 and Swiss-Hungarian grant (SH/7/2/8) for additional support. We thank the Cell Biology Center (Flow Cytometry Core Facility) at the Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest, Hungary for their assistance and Csaba D?vid for his help in the development of macros used for analysis of microglia process coverage. We thank L?szl? Barna, Vivien Micz?n and the NMC Imaging Center at the Institute of Experimental Medicine for kindly providing microscopy support. Funding details: KTIA-13-NAP-A-I/2 Funding details: Horizon 2020 Framework Programme, H2020 Funding details: Seventh Framework Programme, FP7, 323945 Funding details: European Research Council, ERC, 682426, KTIA-NAP-13-2-2015-0006, SH/7/2/8 Funding details: Hungarian Scientific Research Fund, OTKA, K109743 Funding details: Magyar Tudományos Akadémia, MTA, FP7-ICT-2011-C 323945, K105997, T?T-10-1-2011-0389, VKSZ-14-1-2015-0155 Funding text 1: Funding was provided by OTKA K109743 (A.D.) and the Hungarian Brain Research Program KTIA-13-NAP-A-I/2 (A.D.). A.D. is supported by the Bolyai Janos Research Scholarship from the Hungarian Academy of Sciences. The authors thank the Hungarian-French grant (T?T-10-1-2011-0389), OTKA (K105997), FP7-ICT-2011-C 323945 (3 ? 3Dimaging), VKSZ-14-1-2015-0155, ERC 682426, KTIA-NAP-13-2-2015-0006 and Swiss-Hungarian grant (SH/7/2/8) for additional support. AB - Microglia are the main immune cells of the brain and contribute to common brain diseases. However, it is unclear how microglia influence neuronal activity and survival in the injured brain in vivo. Here we develop a precisely controlled model of brain injury induced by cerebral ischaemia combined with fast in vivo two-photon calcium imaging and selective microglial manipulation. We show that selective elimination of microglia leads to a striking, 60% increase in infarct size, which is reversed by microglial repopulation. Microglia-mediated protection includes reduction of excitotoxic injury, since an absence of microglia leads to dysregulated neuronal calcium responses, calcium overload and increased neuronal death. Furthermore, the incidence of spreading depolarization (SD) is markedly reduced in the absence of microglia. Thus, microglia are involved in changes in neuronal network activity and SD after brain injury in vivo that could have important implications for common brain diseases. LA - English DB - MTMT ER - TY - JOUR AU - Lénárt, Nikolett AU - Walter, Fruzsina AU - Bocsik, Alexandra AU - Sántha, Petra AU - Tóth, Erzsébet Melinda AU - Harazin, András AU - Tóth, Andrea AU - Vizler, Csaba AU - Török, Zsolt AU - Pilbat, Ana Maria AU - Vigh, László AU - Puskás, László AU - Sántha, Miklós AU - Deli, Mária Anna TI - Cultured cells of the blood-brain barrier from apolipoprotein B-100 transgenic mice: effects of oxidized low-density lipoprotein treatment JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 12 PY - 2015 PG - 16 SN - 2045-8118 DO - 10.1186/s12987-015-0013-y UR - https://m2.mtmt.hu/api/publication/2920970 ID - 2920970 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Erzsébet Melinda AU - Szegedi, Viktor AU - Varga, Edina AU - Juhász, Gábor AU - Horváth, János AU - Borbély, Emőke AU - Csibrány, Balázs AU - Alföldi, Róbert AU - Lénárt, Nikolett AU - Penke, Botond AU - Sántha, Miklós TI - Overexpression of Hsp27 ameliorates symptoms of Alzheimer's disease in APP/PS1 mice JF - CELL STRESS & CHAPERONES J2 - CELL STRESS CHAPERON VL - 18 PY - 2013 IS - 6 SP - 759 EP - 771 PG - 13 SN - 1355-8145 DO - 10.1007/s12192-013-0428-9 UR - https://m2.mtmt.hu/api/publication/2365063 ID - 2365063 AB - Hsp27 belongs to the small heat shock protein family, which are ATP-independent chaperones. The most important function of Hsp27 is based on its ability to bind non-native proteins and inhibit the aggregation of incorrectly folded proteins maintaining them in a refolding-competent state. Additionally, it has anti-apoptotic and antioxidant activities. To study the effect of Hsp27 on memory and synaptic functions, amyloid-β (Aβ) accumulation, and neurodegeneration, we generated transgenic mice overexpressing human Hsp27 protein and crossed with APPswe/PS1dE9 mouse strain, a mouse model of Alzheimer's disease (AD). Using different behavioral tests, we found that spatial learning was impaired in AD model mice and was rescued by Hsp27 overexpression. Electrophysiological recordings have revealed that excitability of neurons was significantly increased, and long-term potentiation (LTP) was impaired in AD model mice, whereas they were normalized in Hsp27 overexpressing AD model mice. Using anti-amyloid antibody, we counted significantly less amyloid plaques in the brain of APPswe/PS1dE9/Hsp27 animals compared to AD model mice. These results suggest that overexpression of Hsp27 protein might ameliorate certain symptoms of AD. © 2013 Cell Stress Society International. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Erzsébet Melinda AU - Gonda, Szilvia AU - Vigh, László AU - Sántha, Miklós TI - Neuroprotective effect of small heat shock protein, Hsp27, after acute and chronic alcohol administration. JF - CELL STRESS & CHAPERONES J2 - CELL STRESS CHAPERON VL - 15 PY - 2010 IS - 6 SP - 807 EP - 817 PG - 11 SN - 1355-8145 DO - 10.1007/s12192-010-0188-8 UR - https://m2.mtmt.hu/api/publication/1921491 ID - 1921491 LA - English DB - MTMT ER - TY - JOUR AU - Nakamoto, H AU - Vigh, László TI - The Small Heat Shock Proteins And Their Clients JF - CELLULAR AND MOLECULAR LIFE SCIENCES J2 - CELL MOL LIFE SCI VL - 64 PY - 2007 IS - 3 SP - 294 EP - 306 PG - 13 SN - 1420-682X DO - 10.1007/s00018-006-6321-2 UR - https://m2.mtmt.hu/api/publication/1915070 ID - 1915070 N1 - Funding Agency and Grant Number: FOGARTY INTERNATIONAL CENTERUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH Fogarty International Center (FIC) [R03TW006154] Funding Source: NIH RePORTER; FIC NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH Fogarty International Center (FIC) [1 R03 TW006154-01A1] Funding Source: Medline LA - English DB - MTMT ER -